Starting /dee2/code/volunteer_pipeline.sh ERR5262780 current disk space = 1551602253824 free memory = 1607235620 ERR5262780 SRAfilesize 8d437a4520e184e54a4e710ef3b0cb82 ERR5262780.sra ERR5262780.sra file validated ERR5262780 is paired end ERR5262780 is conventional basespace ERR5262780 read1 length is 77-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262780_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 77-150 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5595 37.0 37.0 37.0 37.0 37.0 2 36.409 37.0 37.0 37.0 37.0 37.0 3 36.5745 37.0 37.0 37.0 37.0 37.0 4 36.7395 37.0 37.0 37.0 37.0 37.0 5 36.658 37.0 37.0 37.0 37.0 37.0 6 36.63 37.0 37.0 37.0 37.0 37.0 7 36.7075 37.0 37.0 37.0 37.0 37.0 8 36.6735 37.0 37.0 37.0 37.0 37.0 9 36.7075 37.0 37.0 37.0 37.0 37.0 10-14 36.6684 37.0 37.0 37.0 37.0 37.0 15-19 36.62 37.0 37.0 37.0 37.0 37.0 20-24 36.617000000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.577600000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.5708 37.0 37.0 37.0 37.0 37.0 35-39 36.55030000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.6184 37.0 37.0 37.0 37.0 37.0 45-49 36.5641 37.0 37.0 37.0 37.0 37.0 50-54 36.5982 37.0 37.0 37.0 37.0 37.0 55-59 36.57950000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.5773 37.0 37.0 37.0 37.0 37.0 65-69 36.5945 37.0 37.0 37.0 37.0 37.0 70-74 36.5837 37.0 37.0 37.0 37.0 37.0 75-79 36.55075723930983 37.0 37.0 37.0 37.0 37.0 80-84 36.561819576735566 37.0 37.0 37.0 37.0 37.0 85-89 36.57848386289717 37.0 37.0 37.0 37.0 37.0 90-94 36.49584716064576 37.0 37.0 37.0 37.0 37.0 95-99 36.499350163680575 37.0 37.0 37.0 37.0 37.0 100-104 36.513265818627815 37.0 37.0 37.0 37.0 37.0 105-109 36.5441413051039 37.0 37.0 37.0 37.0 37.0 110-114 36.49360483506915 37.0 37.0 37.0 37.0 37.0 115-119 36.461237439985226 37.0 37.0 37.0 37.0 37.0 120-124 36.44325934661156 37.0 37.0 37.0 37.0 37.0 125-129 36.48419881671547 37.0 37.0 37.0 37.0 37.0 130-134 36.415184839400304 37.0 37.0 37.0 37.0 37.0 135-139 36.37833837455783 37.0 37.0 37.0 37.0 37.0 140-144 36.30499986437644 37.0 37.0 37.0 37.0 37.0 145-149 36.27557016650545 37.0 37.0 37.0 37.0 37.0 150 36.26039260969977 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 0.0 24 1.0 25 1.0 26 0.0 27 1.0 28 3.0 29 2.0 30 12.0 31 13.0 32 39.0 33 47.0 34 79.0 35 204.0 36 2785.0 37 811.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 48.55 16.025 5.6000000000000005 29.825000000000003 2 30.955955955955954 18.96896896896897 26.526526526526528 23.54854854854855 3 22.55 21.925 24.6 30.925000000000004 4 29.45 23.9 16.1 30.55 5 26.525 33.425 14.924999999999999 25.124999999999996 6 21.825 37.5 20.175 20.5 7 22.35 28.7 30.325000000000003 18.625 8 19.475 34.150000000000006 24.775 21.6 9 22.425 18.475 27.075 32.025 10-14 23.974999999999998 29.255 21.38 25.39 15-19 21.535 26.950000000000003 21.32 30.195 20-24 24.125 29.445 20.155 26.275 25-29 27.134999999999998 30.895 16.035 25.935000000000002 30-34 22.509999999999998 29.15 20.72 27.62 35-39 20.265 32.025 20.06 27.650000000000002 40-44 19.6 34.150000000000006 19.285 26.965 45-49 20.185 32.17 19.139999999999997 28.505000000000003 50-54 22.93 34.185 20.135 22.75 55-59 18.01 32.87 20.715 28.405 60-64 21.615000000000002 28.89 23.625 25.869999999999997 65-69 23.505000000000003 34.64 17.125 24.73 70-74 21.654999999999998 31.619999999999997 17.115 29.609999999999996 75-79 23.347334733473346 27.87778777877788 24.087408740874086 24.68746874687469 80-84 22.67473858007705 25.966878471006154 23.975584129684293 27.3827988192325 85-89 23.412559419564673 25.339004253189895 24.38829121841381 26.860145108831624 90-94 19.43749374436993 25.512961665498953 28.931037934140726 26.11850665599039 95-99 19.551506657323056 24.13655020522575 27.930723796175794 28.381219341275404 100-104 21.322719535396015 25.823570641834387 25.413036948032442 27.44067287473716 105-109 17.724947336743906 22.32922058380981 28.964790851640082 30.981041227806198 110-114 21.169140367762523 21.645306722050556 27.64297654627425 29.54257636391267 115-119 26.910401647785786 20.020597322348095 24.598352214212152 28.470648815653966 120-124 25.709071949947866 24.124087591240876 22.30448383733055 27.86235662148071 125-129 18.93093410955279 28.5260650965864 23.12251918496957 29.42048160889124 130-134 18.900768280234246 27.464675226991886 21.447375490248753 32.187181002525115 135-139 22.1041678076565 21.342899392080618 25.757160852182487 30.795771948080397 140-144 26.39919647341108 23.83237542547849 20.0044640366051 29.763964064505327 145-149 28.59499431171786 25.119453924914676 18.623435722411834 27.66211604095563 150 24.047344110854503 29.070438799076214 22.08429561200924 24.797921478060044 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 1.0 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.5 34 1.0 35 3.0 36 5.5 37 12.0 38 29.5 39 154.0 40 282.5 41 350.0 42 363.5 43 377.5 44 544.0 45 477.5 46 251.0 47 132.0 48 33.0 49 4.5 50 7.0 51 6.5 52 0.5 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 3.5 63 32.5 64 60.5 65 56.5 66 26.0 67 7.0 68 44.0 69 72.5 70 74.0 71 63.0 72 63.0 73 139.0 74 188.0 75 110.0 76 21.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 76-77 1.0 78-79 0.0 80-81 2.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.0 92-93 0.0 94-95 0.0 96-97 1.0 98-99 0.0 100-101 0.0 102-103 1.0 104-105 2.0 106-107 9.0 108-109 14.0 110-111 23.0 112-113 14.0 114-115 44.0 116-117 13.0 118-119 21.0 120-121 19.0 122-123 14.0 124-125 30.0 126-127 25.0 128-129 22.0 130-131 17.0 132-133 34.0 134-135 26.0 136-137 31.0 138-139 24.0 140-141 28.0 142-143 27.0 144-145 28.0 146-147 23.0 148-149 42.0 150-151 3464.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.85 #Duplication Level Percentage of deduplicated Percentage of total 1 33.26848249027237 4.275 2 18.482490272373543 4.75 3 7.976653696498054 3.075 4 4.280155642023346 2.1999999999999997 5 4.863813229571985 3.125 6 5.447470817120623 4.2 7 2.529182879377432 2.275 8 2.529182879377432 2.6 9 2.140077821011673 2.475 >10 15.369649805447471 42.95 >50 2.9182879377431905 24.525 >100 0.19455252918287938 3.55 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGA 142 3.55 No Hit GTCGGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGAACACGTCGT 93 2.325 No Hit GGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAGT 85 2.125 No Hit GTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAGTTGGCCA 79 1.975 No Hit GTTGCAGACAATGGCATTGTTCTTCATCTTCCTCATGTGGTCAACCATGA 75 1.875 No Hit GAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAA 72 1.7999999999999998 No Hit CGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAA 69 1.725 No Hit AGTGAATACAACAGCTGCCATTGAAGATGTTAAAACCACCCTTTTCAAAG 65 1.625 No Hit CCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACA 59 1.4749999999999999 No Hit CCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAGTTG 58 1.4500000000000002 No Hit GACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAA 58 1.4500000000000002 No Hit GCTCGGTTCAACATGTGATCATAAAAACATCTGCCAGGTAATAAGCAGCA 56 1.4000000000000001 No Hit GATACATATCAACAACGACGAGAAGATGATGACAATACACTGGCCGGCCT 56 1.4000000000000001 No Hit GCTTGATGGTGATGCGCTTGACACCAGGGTAAGTCTCAAGGCCGTTCATG 53 1.325 No Hit GTCACGCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCG 52 1.3 No Hit GTCAATCTCATTGTCAAAGTGACCAATGTTGCAGACAATGGCATTGTTCT 51 1.275 No Hit GTGAATACAACAGCTGCCATTGAAGATGTTAAAACCACCCTTTTCAAAGA 49 1.225 No Hit GCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCCTCGTCTTC 44 1.0999999999999999 No Hit GTTCATGTCAATCTCATTGTCAAAGTGACCAATGTTGCAGACAATGGCAT 44 1.0999999999999999 No Hit AGGAGATAATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTA 43 1.075 No Hit TGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGG 42 1.05 No Hit CCGTCACGCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGC 41 1.0250000000000001 No Hit GATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAA 41 1.0250000000000001 No Hit GGCAGATCAGAGCAAACGGCTCGGTTCAACATGTGATCATAAAAACATCT 40 1.0 No Hit GTCGGACTGCCCACGGACGAGCTTCTCCGCCTCCTGGGCCCGTTCCCCGA 38 0.95 No Hit GATACATGTAATATTTCGGGACGGAGGGGTACTAAATACTTGCCCAAAAG 38 0.95 No Hit GCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAGTT 37 0.9249999999999999 No Hit AGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAG 35 0.8750000000000001 No Hit ATACATATCAACAACGACGAGAAGATGATGACAATACACTGGCCGGCCTT 33 0.8250000000000001 No Hit GGAGATAATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTAA 33 0.8250000000000001 No Hit CGGCAGATCAGAGCAAACGGCTCGGTTCAACATGTGATCATAAAAACATC 32 0.8 No Hit CTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCCTCGT 32 0.8 No Hit ACTAGATACATGTAATATTTCGGGACGGAGGGGTACTAAATACTTGCCCA 32 0.8 No Hit GACCAATGTTGCAGACAATGGCATTGTTCTTCATCTTCCTCATGTGGTCA 31 0.775 No Hit CTAGATACATGTAATATTTCGGGACGGAGGGGTACTAAATACTTGCCCAA 31 0.775 No Hit GTCGTCGGACTGCCCACGGACGAGCTTCTCCGCCTCCTGGGCCCGTTCCC 31 0.775 No Hit CAGGAGATAATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTT 31 0.775 No Hit CACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAA 29 0.7250000000000001 No Hit CGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAG 26 0.65 No Hit CCCGTCACGCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACG 26 0.65 No Hit AGATAATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTAAAA 25 0.625 No Hit CGTCACGCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCC 25 0.625 No Hit GACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGA 24 0.6 No Hit GGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACA 23 0.575 No Hit GAATACAACAGCTGCCATTGAAGATGTTAAAACCACCCTTTTCAAAGAAG 23 0.575 No Hit CTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGGCTCGGTTCAA 23 0.575 No Hit ACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAG 22 0.5499999999999999 No Hit GGCTTGATGGTGATGCGCTTGACACCAGGGTAAGTCTCAAGGCCGTTCAT 21 0.525 No Hit TGTCAATCTCATTGTCAAAGTGACCAATGTTGCAGACAATGGCATTGTTC 20 0.5 No Hit CCACGGACGAGCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTT 20 0.5 No Hit GCAGACAATGGCATTGTTCTTCATCTTCCTCATGTGGTCAACCATGATAA 20 0.5 No Hit GTAATATTTCGGGACGGAGGGGTACTAAATACTTGCCCAAAAGAATTAAA 20 0.5 No Hit ACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAAC 20 0.5 No Hit GGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTAAAACCACCCT 19 0.475 No Hit GCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGGCTCGGTTCAACA 18 0.44999999999999996 No Hit GCGCTGGCGCGGGCCACGGACGAACACGTCGTCGGACTGCCCACGGACGA 17 0.42500000000000004 No Hit CTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAGTTGG 16 0.4 No Hit ATGTAATATTTCGGGACGGAGGGGTACTAAATACTTGCCCAAAAGAATTA 16 0.4 No Hit ATCAACAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCA 16 0.4 No Hit AACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCC 16 0.4 No Hit GCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCTGGCGCTGGC 16 0.4 No Hit CGTCGGACTGCCCACGGACGAGCTTCTCCGCCTCCTGGGCCCGTTCCCCG 16 0.4 No Hit ATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAG 16 0.4 No Hit GCATTATAGAACTCGGCAGATCAGAGCAAACGGCTCGGTTCAACATGTGA 16 0.4 No Hit GGGACGGAGGGGTACTAAATACTTGCCCAAAAGAATTAAACTTGCAGAAT 15 0.375 No Hit AATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGA 15 0.375 No Hit CACGCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCT 15 0.375 No Hit ACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCA 15 0.375 No Hit TCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCCTCGTC 15 0.375 No Hit GGACTGCCCACGGACGAGCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACA 15 0.375 No Hit CTCACATGTCGGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGAAC 15 0.375 No Hit CAGACAATGGCATTGTTCTTCATCTTCCTCATGTGGTCAACCATGATAAT 14 0.35000000000000003 No Hit GTGACCAATGTTGCAGACAATGGCATTGTTCTTCATCTTCCTCATGTGGT 14 0.35000000000000003 No Hit CAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAG 13 0.325 No Hit CGGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGAACACGTCGTCG 13 0.325 No Hit GGCTCGGTTCAACATGTGATCATAAAAACATCTGCCAGGTAATAAGCAGC 13 0.325 No Hit GGCGCTGGCGCGGGCCACGGACGAACACGTCGTCGGACTGCCCACGGACG 13 0.325 No Hit AGACAATGGCATTGTTCTTCATCTTCCTCATGTGGTCAACCATGATAATG 13 0.325 No Hit TGGTGATGCGCTTGACACCAGGGTAAGTCTCAAGGCCGTTCATGTCAATC 13 0.325 No Hit ATGTCAATCTCATTGTCAAAGTGACCAATGTTGCAGACAATGGCATTGTT 13 0.325 No Hit GAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGG 12 0.3 No Hit CTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACA 12 0.3 No Hit CCACGGACGAACACGTCGTCGGACTGCCCACGGACGAGCTTCTCCGCCTC 12 0.3 No Hit GATGGTGATGCGCTTGACACCAGGGTAAGTCTCAAGGCCGTTCATGTCAA 12 0.3 No Hit ACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAA 12 0.3 No Hit CGACTTCTTATCCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAAC 12 0.3 No Hit GCCGCTCTGGCGCTGGCGCGGGCCACGGACGAACACGTCGTCGGACTGCC 11 0.27499999999999997 No Hit CACATGTCGGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGAACAC 11 0.27499999999999997 No Hit GGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACAC 11 0.27499999999999997 No Hit CAACAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAA 11 0.27499999999999997 No Hit GCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCC 11 0.27499999999999997 No Hit GAGATAATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTAAA 10 0.25 No Hit GTCAAAGTGACCAATGTTGCAGACAATGGCATTGTTCTTCATCTTCCTCA 10 0.25 No Hit GTCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACAC 10 0.25 No Hit ACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAA 10 0.25 No Hit ACACAGTTGGCCACATGATCCATATACGTCAGATCTGGATCGATGTGGTC 9 0.22499999999999998 No Hit GTTCAAAACGACTTCTTATCCTGCAACGCATTATAGAACTCGGCAGATCA 9 0.22499999999999998 No Hit CGGGACGGAGGGGTACTAAATACTTGCCCAAAAGAATTAAACTTGCAGAA 9 0.22499999999999998 No Hit CATATCAACAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTT 9 0.22499999999999998 No Hit TTCTTATCCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGGCT 9 0.22499999999999998 No Hit CAATCTCATTGTCAAAGTGACCAATGTTGCAGACAATGGCATTGTTCTTC 9 0.22499999999999998 No Hit CCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAGTTGGCCACAT 9 0.22499999999999998 No Hit ATACATGTAATATTTCGGGACGGAGGGGTACTAAATACTTGCCCAAAAGA 9 0.22499999999999998 No Hit GGACGAGCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTG 9 0.22499999999999998 No Hit AATACAACAGCTGCCATTGAAGATGTTAAAACCACCCTTTTCAAAGAAGA 9 0.22499999999999998 No Hit CCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGGCTCGGTTCA 9 0.22499999999999998 No Hit CGGCTCGGTTCAACATGTGATCATAAAAACATCTGCCAGGTAATAAGCAG 8 0.2 No Hit GACTTCTTATCCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACG 8 0.2 No Hit GAGCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCG 8 0.2 No Hit AGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAA 8 0.2 No Hit CGCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCT 8 0.2 No Hit CGTCGTCGGACTGCCCACGGACGAGCTTCTCCGCCTCCTGGGCCCGTTCC 8 0.2 No Hit TGATGGTGATGCGCTTGACACCAGGGTAAGTCTCAAGGCCGTTCATGTCA 8 0.2 No Hit AGCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGC 8 0.2 No Hit AGGGGTACTAAATACTTGCCCAAAAGAATTAAACTTGCAGAATCAGGTAG 8 0.2 No Hit CGGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACAC 8 0.2 No Hit GTTCGGGACGGAGGGGTACTAAATACTTGCCCAAAAGAATTAAACTTGCA 8 0.2 No Hit TCAACAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAA 8 0.2 No Hit TCAGTGAATACAACAGCTGCCATTGAAGATGTTAAAACCACCCTTTTCAA 8 0.2 No Hit ATCAGAGCAAACGGCTCGGTTCAACATGTGATCATAAAAACATCTGCCAG 7 0.17500000000000002 No Hit CCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCCTCGTCT 7 0.17500000000000002 No Hit AGATACATGTAATATTTCGGGACGGAGGGGTACTAAATACTTGCCCAAAA 7 0.17500000000000002 No Hit CTGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACA 7 0.17500000000000002 No Hit ACGACTTCTTATCCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAA 7 0.17500000000000002 No Hit ATATCAACAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTC 7 0.17500000000000002 No Hit GCCACGGACGAACACGTCGTCGGACTGCCCACGGACGAGCTTCTCCGCCT 7 0.17500000000000002 No Hit GGGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAA 7 0.17500000000000002 No Hit ACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCTGGC 7 0.17500000000000002 No Hit CATGTCGGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGAACACGT 7 0.17500000000000002 No Hit AACAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAAT 7 0.17500000000000002 No Hit ACTTCTTATCCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGG 7 0.17500000000000002 No Hit GCGCGGGCCACGGACGAACACGTCGTCGGACTGCCCACGGACGAGCTTCT 7 0.17500000000000002 No Hit GTGGCATTGTTCTTCATCTTCCTCATGTGGTCAACCATGATAATGTCCTT 6 0.15 No Hit ATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTAAAACCACC 6 0.15 No Hit CACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCTGG 6 0.15 No Hit CTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCCT 6 0.15 No Hit GGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGAACACGTCGTCGG 6 0.15 No Hit CGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCTGGCGCTGGCGCG 6 0.15 No Hit TGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTAAAACCACCC 6 0.15 No Hit GTCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCTGGCGCTGGC 6 0.15 No Hit CGGCGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAA 6 0.15 No Hit GCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCTG 6 0.15 No Hit CAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTC 6 0.15 No Hit GTCCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGGCTCGGTT 6 0.15 No Hit TGAATACAACAGCTGCCATTGAAGATGTTAAAACCACCCTTTTCAAAGAA 6 0.15 No Hit CACGGACGAACACGTCGTCGGACTGCCCACGGACGAGCTTCTCCGCCTCC 6 0.15 No Hit GCTCACATGTCGGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGAA 6 0.15 No Hit GCAGATCAGAGCAAACGGCTCGGTTCAACATGTGATCATAAAAACATCTG 6 0.15 No Hit CAAGAATAGAACTAGTATCCACACGTGCTGCCCGATACTGTACCGAAAGA 6 0.15 No Hit ACGCACCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTC 6 0.15 No Hit CGGCATTGTTCTTCATCTTCCTCATGTGGTCAACCATGATAATGTCCTTG 6 0.15 No Hit GACGAGCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGG 6 0.15 No Hit CATTAATGTTCAAAACGACTTCTTATCCTGCAACGCATTATAGAACTCGG 6 0.15 No Hit GCGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAG 6 0.15 No Hit CACGGACGAGCTTCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTC 6 0.15 No Hit AAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCAAAGAAGGA 6 0.15 No Hit CGGCGCTGGCGCGGGCCACGGACGAACACGTCGTCGGACTGCCCACGGAC 6 0.15 No Hit CATTGTCAAAGTGACCAATGTTGCAGACAATGGCATTGTTCTTCATCTTC 6 0.15 No Hit CAGTGAATACAACAGCTGCCATTGAAGATGTTAAAACCACCCTTTTCAAA 6 0.15 No Hit GGGGATAATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGATGTTAA 6 0.15 No Hit TCTCCGCCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCCTCG 5 0.125 No Hit GGGCATTGTTCTTCATCTTCCTCATGTGGTCAACCATGATAATGTCCTTG 5 0.125 No Hit CTCGGCAGATCAGAGCAAACGGCTCGGTTCAACATGTGATCATAAAAACA 5 0.125 No Hit GGCCACGGACGAACACGTCGTCGGACTGCCCACGGACGAGCTTCTCCGCC 5 0.125 No Hit GTCTCCTGGGCCCGTTCCCCGAACACGAGCTTCCTGGCCGCCTCGTCTTC 5 0.125 No Hit CTTGATGGTGATGCGCTTGACACCAGGGTAAGTCTCAAGGCCGTTCATGT 5 0.125 No Hit GCGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATTCCA 5 0.125 No Hit CGGGCCACGGACGAACACGTCGTCGGACTGCCCACGGACGAGCTTCTCCG 5 0.125 No Hit GCAACGACGAGAAGATGATGACAATACACTGGCCGGCCTTGTTCCAAATT 5 0.125 No Hit CTCTGGCGCTGGCGCGGGCCACGGACGAACACGTCGTCGGACTGCCCACG 5 0.125 No Hit CTTCAGGAGATAATGGATTTTCAGTGAATACAACAGCTGCCATTGAAGAT 5 0.125 No Hit GCTGGCGCGGGCCACGGACGAACACGTCGTCGGACTGCCCACGGACGAGC 5 0.125 No Hit GATCAGAGCAAACGGCTCGGTTCAACATGTGATCATAAAAACATCTGCCA 5 0.125 No Hit GCCAATGTTGCAGACAATGGCATTGTTCTTCATCTTCCTCATGTGGTCAA 5 0.125 No Hit CGCTCACATGTCGGCAACGCCGCTCTGGCGCTGGCGCGGGCCACGGACGA 5 0.125 No Hit GTCGATGATACCAGCTGAAGAATAGTTGAAAATGTCCTATGGTGCGACAT 5 0.125 No Hit CTTCTTATCCTGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGGC 5 0.125 No Hit GTGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACAC 5 0.125 No Hit GCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACA 5 0.125 No Hit AGCCGGCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACA 5 0.125 No Hit TGCAACGCATTATAGAACTCGGCAGATCAGAGCAAACGGCTCGGTTCAAC 5 0.125 No Hit GTACTAAATACTTGCCCAAAAGAATTAAACTTGCAGAATCAGGTAGTCCA 5 0.125 No Hit GGACGAACACGTCGTCGGACTGCCCACGGACGAGCTTCTCCGCCTCCTGG 5 0.125 No Hit GGGGTACTAAATACTTGCCCAAAAGAATTAAACTTGCAGAATCAGGTAGT 5 0.125 No Hit CTCCAGGCCCGCCGCTCCGACCGCTCACATGTCGGCAACGCCGCTCTGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATGGT 10 0.0072561596 142.1 4 ATGGTGA 10 0.0072561596 142.1 6 TTGATGG 10 0.0072561596 142.1 3 GCTTGAT 10 0.0072561596 142.1 1 CTTGATG 10 0.0072561596 142.1 2 GATGGTG 10 0.0072561596 142.1 5 CCATCCA 30 8.330955E-4 26.535948 140-144 TACGGCC 35 0.0024050474 22.125341 135-139 ACGGCCA 50 8.506419E-4 18.585285 135-139 ATGACAA 75 8.24524E-5 15.157332 10-14 AATACAC 85 2.3913351E-4 13.374118 15-19 GATGACA 75 0.0014415825 13.262667 10-14 TGACAAT 80 0.0023432767 12.43375 10-14 ACTGGCC 95 6.118078E-4 11.966316 20-24 CAATACA 85 0.0036915939 11.7023535 15-19 ATACACT 85 0.0036915939 11.7023535 15-19 CACTGGC 90 0.0056567397 11.052222 20-24 CCGGCCT 110 0.0020869481 10.334545 25-29 TCCAGGG 125 0.0023252969 10.189804 140-144 CCTGGTC 130 0.0040568826 9.530916 135-139 >>END_MODULE ERR5262780 read2 length is 77-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262780_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 77-150 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0895 37.0 37.0 37.0 37.0 37.0 2 36.0335 37.0 37.0 37.0 37.0 37.0 3 35.9745 37.0 37.0 37.0 37.0 37.0 4 36.1645 37.0 37.0 37.0 37.0 37.0 5 36.2235 37.0 37.0 37.0 37.0 37.0 6 36.2515 37.0 37.0 37.0 37.0 37.0 7 36.156 37.0 37.0 37.0 37.0 37.0 8 36.298 37.0 37.0 37.0 37.0 37.0 9 36.2845 37.0 37.0 37.0 37.0 37.0 10-14 36.28000000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.36130000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.26200000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.2861 37.0 37.0 37.0 37.0 37.0 30-34 36.1957 37.0 37.0 37.0 37.0 37.0 35-39 36.165800000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.1761 37.0 37.0 37.0 37.0 37.0 45-49 36.2667 37.0 37.0 37.0 37.0 37.0 50-54 36.129400000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.04259999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.0549 37.0 37.0 37.0 37.0 37.0 65-69 36.0818 37.0 37.0 37.0 37.0 37.0 70-74 36.0184 37.0 37.0 37.0 37.0 37.0 75-79 36.09400205051263 37.0 37.0 37.0 37.0 37.0 80-84 35.99834438219262 37.0 37.0 37.0 37.0 37.0 85-89 35.962922191643734 37.0 37.0 37.0 37.0 37.0 90-94 35.962763839646506 37.0 37.0 37.0 37.0 37.0 95-99 35.94123906133918 37.0 37.0 37.0 37.0 37.0 100-104 35.90437638936503 37.0 37.0 37.0 37.0 37.0 105-109 35.85423779064614 37.0 37.0 37.0 37.0 37.0 110-114 35.83767097196592 37.0 37.0 37.0 37.0 37.0 115-119 35.81675595046741 37.0 37.0 37.0 37.0 37.0 120-124 35.76212469184755 37.0 37.0 37.0 37.0 37.0 125-129 35.79860151362297 37.0 37.0 37.0 37.0 37.0 130-134 35.73338749179259 37.0 37.0 37.0 37.0 37.0 135-139 35.65905907922414 37.0 37.0 37.0 37.0 37.0 140-144 35.71930542013092 37.0 37.0 37.0 37.0 37.0 145-149 35.60649064053151 37.0 37.0 37.0 37.0 37.0 150 35.558772437753326 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 1.0 13 0.0 14 0.0 15 1.0 16 1.0 17 2.0 18 2.0 19 1.0 20 6.0 21 2.0 22 5.0 23 6.0 24 8.0 25 6.0 26 6.0 27 5.0 28 6.0 29 12.0 30 18.0 31 35.0 32 42.0 33 63.0 34 167.0 35 521.0 36 2822.0 37 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.425 19.625 5.949999999999999 21.0 2 34.55 19.5 27.375 18.575 3 24.525 25.174999999999997 32.725 17.575 4 32.074999999999996 27.900000000000002 20.200000000000003 19.825 5 34.075 27.125 21.375 17.424999999999997 6 33.175 30.099999999999998 16.85 19.875 7 24.975 17.4 34.849999999999994 22.775000000000002 8 29.525000000000002 20.05 25.95 24.474999999999998 9 28.925 19.025 29.2 22.85 10-14 31.28 22.58 23.52 22.62 15-19 30.964999999999996 20.89 25.035 23.11 20-24 30.104999999999997 21.555 22.93 25.41 25-29 31.47 21.83 22.175 24.525 30-34 31.365 22.994999999999997 21.615000000000002 24.025 35-39 31.605 22.314999999999998 23.24 22.84 40-44 31.075000000000003 22.305 26.02 20.599999999999998 45-49 33.575 21.044999999999998 23.95 21.43 50-54 32.09 21.065 23.31 23.535 55-59 31.879999999999995 21.985 24.795 21.34 60-64 30.55 22.755 24.279999999999998 22.415 65-69 31.055 22.21 24.91 21.825 70-74 30.43 21.935 26.32 21.315 75-79 30.663066306630665 21.027102710271027 26.527652765276528 21.782178217821784 80-84 30.169610246660326 21.533997098113776 26.352128883774455 21.944263771451446 85-89 30.28771578684013 21.015761821366024 24.628471353515135 24.06805103827871 90-94 29.82684415974377 22.089880892803524 25.938344510059054 22.144930437393654 95-99 31.374511963159474 22.274501952147364 25.362899189108017 20.98808689558514 100-104 29.878842495243816 23.059977971362773 24.957444678081504 22.103734855311906 105-109 28.317785133915134 23.402547898485302 25.66957568462233 22.61009128297723 110-114 28.149536497644494 22.308900258345577 28.07355250493896 21.46801073907097 115-119 28.60453141091658 21.98249227600412 28.352214212152422 21.06076210092688 120-124 28.34723670490094 25.599582898852972 24.191866527632953 21.861313868613138 125-129 28.039163799947076 23.995766075681395 26.308547234718176 21.65652288965335 130-134 28.29205394079407 23.53731263095686 25.460699511094397 22.709933917154675 135-139 30.047128452433142 21.448925909688732 24.33691363437089 24.167032003507234 140-144 30.695336498184865 20.68137391790003 26.003909522479756 22.619380061435354 145-149 30.116909039064726 22.4009124607927 25.68577131451383 21.79640718562874 150 28.807180081065432 19.137232194557036 31.32599884192241 20.729588882455126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 0.0 25 0.5 26 0.5 27 0.0 28 1.5 29 1.5 30 2.5 31 2.5 32 1.0 33 3.0 34 7.0 35 11.0 36 27.0 37 41.0 38 74.5 39 97.5 40 84.5 41 130.5 42 152.0 43 100.5 44 76.5 45 109.5 46 150.0 47 275.0 48 325.0 49 179.0 50 102.5 51 132.5 52 115.0 53 85.5 54 112.5 55 120.5 56 125.5 57 91.5 58 44.0 59 36.5 60 40.0 61 33.5 62 18.0 63 34.0 64 58.5 65 67.5 66 57.0 67 67.5 68 99.5 69 82.0 70 76.5 71 76.5 72 52.0 73 46.5 74 118.0 75 161.0 76 75.0 77 10.0 78 1.0 79 0.5 80 1.0 81 1.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 76-77 1.0 78-79 0.0 80-81 2.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.0 92-93 0.0 94-95 0.0 96-97 1.0 98-99 0.0 100-101 0.0 102-103 1.0 104-105 2.0 106-107 9.0 108-109 14.0 110-111 23.0 112-113 14.0 114-115 44.0 116-117 13.0 118-119 21.0 120-121 19.0 122-123 14.0 124-125 30.0 126-127 25.0 128-129 22.0 130-131 17.0 132-133 34.0 134-135 28.0 136-137 32.0 138-139 24.0 140-141 28.0 142-143 28.0 144-145 34.0 146-147 23.0 148-149 42.0 150-151 3454.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.375 #Duplication Level Percentage of deduplicated Percentage of total 1 46.38297872340426 13.625000000000002 2 16.851063829787233 9.9 3 10.72340425531915 9.45 4 6.6382978723404245 7.8 5 4.25531914893617 6.25 6 3.0638297872340425 5.4 7 2.382978723404255 4.9 8 1.9574468085106382 4.6 9 1.1063829787234043 2.9250000000000003 >10 6.297872340425532 28.549999999999997 >50 0.3404255319148936 6.6000000000000005 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGC 74 1.8499999999999999 No Hit GTCGGAAATAAAAGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTT 72 1.7999999999999998 No Hit GGAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGG 62 1.55 No Hit GTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGGTAATAAGAGACC 56 1.4000000000000001 No Hit CAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGC 31 0.775 No Hit GCATCGACTGCCATGGATTGGATGGATGGATATGCTGTGGGACAGTCGGA 28 0.7000000000000001 No Hit AGTGGATATGCATCGACTGCCATGGATTGGATGGATGGATATGCTGTGGG 25 0.625 No Hit GTGAAGCCGAGCATGTGATCCATGAGAGTACAGTGGATATGCATCGACTG 25 0.625 No Hit GGAGGATGGTAATAAGAGACCACATCGATCCAGATCTGACGTATATGGAT 25 0.625 No Hit AGAACAGCCCGCTGAGGCAGCTGGTGGAGGAAGACGAGGCGGCCAGGAAG 24 0.6 No Hit GGAAGCTCGTGTTCGGGGAACGGGCCCAGGAGGCGGAGAAGCTCGTCCGT 24 0.6 No Hit CGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGGTAATAAGAGAC 23 0.575 No Hit ATTGGATGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAA 22 0.5499999999999999 No Hit GGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGG 21 0.525 No Hit GTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGATGGGAA 20 0.5 No Hit CGGAAATAAAAGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCT 20 0.5 No Hit CATCGATCCAGATCTGACGTATATGGATCATGTGGCCAACTGTGTGTTTG 20 0.5 No Hit GCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGGTAATAAGAGACCACAT 20 0.5 No Hit GGAAGACGAGGCGGCCAGGAAGCTCGTGTTCGGGGAACGGGCCCAGGAGG 20 0.5 No Hit GCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGATGGGAACC 19 0.475 No Hit AGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGACCA 19 0.475 No Hit GTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGA 19 0.475 No Hit GTCGTGTGGGATGAAGGTTGCGAACCGGCTCAACGTGAAGCCGAGCATGT 18 0.44999999999999996 No Hit CTTCTCCCTGGACCAGGAGGATGGTAATAAGAGACCACATCGATCCAGAT 18 0.44999999999999996 No Hit GCCATGGATTGGATGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGT 17 0.42500000000000004 No Hit GTGATGTTGGCAAGGGCTGTGCTTCTGCGCTCAAGCAGGCTGGTGCCCGT 17 0.42500000000000004 No Hit GTTGACGGTTGTGAATGCGTATTTCATACAGCATCTCCTTTCTACAATAA 17 0.42500000000000004 No Hit GTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGATGGCCGTAAGCTTT 17 0.42500000000000004 No Hit AATGCAGGTTGCTGTTATTGTCCTACGGATTATATTTTTTCTGCGTCTGC 16 0.4 No Hit GTTGTGAATGCGTATTTCATACAGCATCTCCTTTCTACAATAATCCGAAA 16 0.4 No Hit GCGCACCCGGCGACGTTCGTGGCGGGCGGCGATGGGAACCTGGCCGTGCT 16 0.4 No Hit CATGAGCTCCGCGCTATCTGGGTCAACTTTCTCTCCCAGTGTGATAAGTC 15 0.375 No Hit GAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGC 15 0.375 No Hit CGGCGACGTTCGTGGCGGGCGGCGATGGGAACCTGGCCGTGCTGTGCTTC 15 0.375 No Hit GTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGG 15 0.375 No Hit GGTGATGTTGGCAAGGGCTGTGCTTCTGCGCTCAAGCAGGCTGGTGCCCG 15 0.375 No Hit GCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGGT 14 0.35000000000000003 No Hit GAACAGCCCGCTGAGGCAGCTGGTGGAGGAAGACGAGGCGGCCAGGAAGC 14 0.35000000000000003 No Hit CTTCAGGCCTTGATGGAGGGTCTCCAGATCCTCACCTTGGAGGATGTTGT 14 0.35000000000000003 No Hit CGTGAAGCCGAGCATGTGATCCATGAGAGTACAGTGGATATGCATCGACT 14 0.35000000000000003 No Hit CACCCGGCGACGTTCGTGGCGGGCGGCGATGGGAACCTGGCCGTGCTGTG 14 0.35000000000000003 No Hit GGATGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCT 14 0.35000000000000003 No Hit GGATTGGATGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTT 14 0.35000000000000003 No Hit GATGAAGGTTGCGAACCGGCTCAACGTGAAGCCGAGCATGTGATCCATGA 14 0.35000000000000003 No Hit TGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGAT 13 0.325 No Hit CCCATCTGTGCCCTTCAGGCCTTGATGGAGGGTCTCCAGATCCTCACCTT 13 0.325 No Hit GGTTGCGAACCGGCTCAACGTGAAGCCGAGCATGTGATCCATGAGAGTAC 13 0.325 No Hit GTTCAAACCAGAAAGGAAGGCAATCTTTCGTGAAAGCGCTGGAGCCTTTT 13 0.325 No Hit AAGGAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGT 13 0.325 No Hit CATCGACTGCCATGGATTGGATGGATGGATATGCTGTGGGACAGTCGGAA 13 0.325 No Hit GTGATCCATGAGAGTACAGTGGATATGCATCGACTGCCATGGATTGGATG 13 0.325 No Hit GGCATCCCAAGGTTAGCCATGAGCTCCGCGCTATCTGGGTCAACTTTCTC 13 0.325 No Hit GGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGAT 13 0.325 No Hit GGAAGAATAAATTGTGCTACCGAAGATCCCTTGATGTTGCTTTCAGTATT 13 0.325 No Hit ATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGATGGCCGTAA 13 0.325 No Hit GGAACCTGGCCGTGCTGTGCTTCGGCGTCGGCGCGGAGCGCGACGAGAAG 13 0.325 No Hit GACAGTCGGAAATAAAAGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGC 12 0.3 No Hit GGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGATGGCC 12 0.3 No Hit GGAATCCGGCACCCTCCTCTTCCCTGCCATCAACGTCAACGACTCCGTCA 12 0.3 No Hit ATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGATGG 12 0.3 No Hit GTTGCGAACCGGCTCAACGTGAAGCCGAGCATGTGATCCATGAGAGTACA 12 0.3 No Hit GATAAGTCGTGTGGGATGAAGGTTGCGAACCGGCTCAACGTGAAGCCGAG 11 0.27499999999999997 No Hit GGAGGGTCTCCAGATCCTCACCTTGGAGGATGTTGTCTCAGAGGCTGACA 11 0.27499999999999997 No Hit GCCCGCTGAGGCAGCTGGTGGAGGAAGACGAGGCGGCCAGGAAGCTCGTG 11 0.27499999999999997 No Hit AAAGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGAC 11 0.27499999999999997 No Hit GCTTCAGCCCAGCCCTTCTTCTCAAAACTGAAAAGAAACACGCATGGAGA 11 0.27499999999999997 No Hit GTTCGTGGCGGGCGGCGATGGGAACCTGGCCGTGCTGTGCTTCGGCGTCG 11 0.27499999999999997 No Hit GCGACGTTCGTGGCGGGCGGCGATGGGAACCTGGCCGTGCTGTGCTTCGG 11 0.27499999999999997 No Hit GGTGTTTTTAACGGGGAAGAACAGCCCGCTGAGGCAGCTGGTGGAGGAAG 11 0.27499999999999997 No Hit ACTTTCTCTCCCAGTGTGATAAGTCGTGTGGGATGAAGGTTGCGAACCGG 11 0.27499999999999997 No Hit GAAGAGGTTCGCGGATGCTTTGGGGCATCCCAAGGTTAGCCATGAGCTCC 11 0.27499999999999997 No Hit GTTATGGTGATGTTGGCAAGGGCTGTGCTTCTGCGCTCAAGCAGGCTGGT 11 0.27499999999999997 No Hit GTACTTGCAAATGCAGGTTGCTGTTATTGTCCTACGGATTATATTTTTTC 11 0.27499999999999997 No Hit CTTCAGCCCAGCCCTTCTTCTCAAAACTGAAAAGAAACACGCATGGAGAT 10 0.25 No Hit GCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGATGGCCGTAAGC 10 0.25 No Hit CCAGATCCTCACCTTGGAGGATGTTGTCTCAGAGGCTGACATCTTTGTGA 10 0.25 No Hit GTGTCATTGTTACTGAGATTGATCCCATCTGTGCCCTTCAGGCCTTGATG 10 0.25 No Hit GATCCATGAGAGTACAGTGGATATGCATCGACTGCCATGGATTGGATGGA 10 0.25 No Hit GTTATTGTCCTACGGATTATATTTTTTCTGCGTCTGCGATATTGTTTACT 9 0.22499999999999998 No Hit GTGTTTTTAACGGGGAAGAACAGCCCGCTGAGGCAGCTGGTGGAGGAAGA 9 0.22499999999999998 No Hit GACGAGGCGGCCAGGAAGCTCGTGTTCGGGGAACGGGCCCAGGAGGCGGA 9 0.22499999999999998 No Hit ATTTCATACAGCATCTCCTTTCTACAATAATCCGAAAGATCCTCAGATTG 9 0.22499999999999998 No Hit GATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGATG 9 0.22499999999999998 No Hit CAAGGAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCG 9 0.22499999999999998 No Hit AGATACCATACTTCTTTGGTTCCGTAAGGGTTCAAACCAGAAAGGAAGGC 9 0.22499999999999998 No Hit AGCTGGTGGAGGAAGACGAGGCGGCCAGGAAGCTCGTGTTCGGGGAACGG 9 0.22499999999999998 No Hit GTTGCTGTTATTGTCCTACGGATTATATTTTTTCTGCGTCTGCGATATTG 9 0.22499999999999998 No Hit GTTGCCGTGGTCTGTGGTTATGGTGATGTTGGCAAGGGCTGTGCTTCTGC 9 0.22499999999999998 No Hit GGCAATCTTTCGTGAAAGCGCTGGAGCCTTTTGTCAAATGGCTTGAAGAG 9 0.22499999999999998 No Hit GATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGATG 9 0.22499999999999998 No Hit GGCGACGTTCGTGGCGGGCGGCGATGGGAACCTGGCCGTGCTGTGCTTCG 9 0.22499999999999998 No Hit GCTTCTGCGCTCAAGCAGGCTGGTGCCCGTGTCATTGTTACTGAGATTGA 8 0.2 No Hit GGATCCTTTGATGCTGTAGTTGACGGTTGTGAATGCGTATTTCATACAGC 8 0.2 No Hit CAGGAGGATGGTAATAAGAGACCACATCGATCCAGATCTGACGTATATGG 8 0.2 No Hit GTCATTGTTACTGAGATTGATCCCATCTGTGCCCTTCAGGCCTTGATGGA 8 0.2 No Hit GCCGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGGTAATAAGAG 8 0.2 No Hit GCTCTTCTCCCTGGACCAGGAGGATGGTAATAAGAGACCACATCGATCCA 8 0.2 No Hit GCCGGCGCCGGCGTGCTTCCCGGTGCCGGTGAGGACCGTGGTTGGGAAGA 8 0.2 No Hit CCGGCGACGTTCGTGGCGGGCGGCGATGGGAACCTGGCCGTGCTGTGCTT 8 0.2 No Hit GCTGTGCTTCGGCGTCGGCGCGGAGCGCGACGAGAAGGTGTTTTTAACGG 8 0.2 No Hit AGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGG 8 0.2 No Hit AATAAAAGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCT 8 0.2 No Hit TTGCAAATGCAGGTTGCTGTTATTGTCCTACGGATTATATTTTTTCTGCG 8 0.2 No Hit CGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGATGGGA 8 0.2 No Hit CATCTGTGCCCTTCAGGCCTTGATGGAGGGTCTCCAGATCCTCACCTTGG 8 0.2 No Hit CCTGGCCGTGCTGTGCTTCGGCGTCGGCGCGGAGCGCGACGAGAAGGTGT 8 0.2 No Hit GTAATAAGAGACCACATCGATCCAGATCTGACGTATATGGATCATGTGGC 8 0.2 No Hit AATAAATTGTGCTACCGAAGATCCCTTGATGTTGCTTTCAGTATTTCCCT 8 0.2 No Hit GGATGGTAATAAGAGACCACATCGATCCAGATCTGACGTATATGGATCAT 8 0.2 No Hit AGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCG 8 0.2 No Hit CGAAGATCCCTTGATGTTGCTTTCAGTATTTCCCTATATCCCATGTCTGT 8 0.2 No Hit GTTGTCTCAGAGGCTGACATCTTTGTGACCACCACTGGAAACAAGGACAT 8 0.2 No Hit ATGAGAGTACAGTGGATATGCATCGACTGCCATGGATTGGATGGATGGAT 8 0.2 No Hit GCGATGGGAACCTGGCCGTGCTGTGCTTCGGCGTCGGCGCGGAGCGCGAC 8 0.2 No Hit GCAACGCGCACTTCGACGGCGCCATGAACTTCATGCACCGGGATGAGGAG 7 0.17500000000000002 No Hit GAGACCACATCGATCCAGATCTGACGTATATGGATCATGTGGCCAACTGT 7 0.17500000000000002 No Hit CCGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGGTAATAAGAGA 7 0.17500000000000002 No Hit GGTCAACTTTCTCTCCCAGTGTGATAAGTCGTGTGGGATGAAGGTTGCGA 7 0.17500000000000002 No Hit GATGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTG 7 0.17500000000000002 No Hit ACAAGGTTCAGACACAGTGCTACGAGGATGCTAAGCTGATGAAGCTGTTC 7 0.17500000000000002 No Hit GAAGCCGAGCATGTGATCCATGAGAGTACAGTGGATATGCATCGACTGCC 7 0.17500000000000002 No Hit GCCGTGCTGTGCTTCGGCGTCGGCGCGGAGCGCGACGAGAAGGTGTTTTT 7 0.17500000000000002 No Hit GCTGTGCTTCTGCGCTCAAGCAGGCTGGTGCCCGTGTCATTGTTACTGAG 7 0.17500000000000002 No Hit TGCGAACCGGCTCAACGTGAAGCCGAGCATGTGATCCATGAGAGTACAGT 7 0.17500000000000002 No Hit AGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTGGCG 7 0.17500000000000002 No Hit GCCGATTTATTAGAAGAAGGATCCTTTGATGCTGTAGTTGACGGTTGTGA 7 0.17500000000000002 No Hit GCCACACCGTGCGCGGCACTCTCCGCGACCCCGCTGATCCGAAGAAGATA 7 0.17500000000000002 No Hit GTGCTTCTGCGCTCAAGCAGGCTGGTGCCCGTGTCATTGTTACTGAGATT 7 0.17500000000000002 No Hit AGGAAGAATAAATTGTGCTACCGAAGATCCCTTGATGTTGCTTTCAGTAT 7 0.17500000000000002 No Hit AGGAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGTTCGTG 7 0.17500000000000002 No Hit CTCCTTTCTACAATAATCCGAAAGATCCTCAGATTGAGTTGATTGACCCA 7 0.17500000000000002 No Hit AGAACATTTACGTGCTCTAGATGGATCTGCCGATAGGCTGCATCTATTTA 7 0.17500000000000002 No Hit GTACAGTGGATATGCATCGACTGCCATGGATTGGATGGATGGATATGCTG 7 0.17500000000000002 No Hit CTCAGATTGAGTTGATTGACCCAGCAGTAAAAGGAACACTCAATGTTCTG 7 0.17500000000000002 No Hit CATCAAGGAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACGT 7 0.17500000000000002 No Hit GAAGACGAGGCGGCCAGGAAGCTCGTGTTCGGGGAACGGGCCCAGGAGGC 7 0.17500000000000002 No Hit GCTGCATCTATTTAAAGCCGATTTATTAGAAGAAGGATCCTTTGATGCTG 7 0.17500000000000002 No Hit GGATGAAGGTTGCGAACCGGCTCAACGTGAAGCCGAGCATGTGATCCATG 7 0.17500000000000002 No Hit GGTAATAAGAGACCACATCGATCCAGATCTGACGTATATGGATCATGTGG 7 0.17500000000000002 No Hit TGTCATTGTTACTGAGATTGATCCCATCTGTGCCCTTCAGGCCTTGATGG 7 0.17500000000000002 No Hit CTTGCAAATGCAGGTTGCTGTTATTGTCCTACGGATTATATTTTTTCTGC 7 0.17500000000000002 No Hit GCGGCGCACCCGGCGACGTTCGTGGCGGGCGGCGATGGGAACCTGGCCGT 7 0.17500000000000002 No Hit ACTTGCAAATGCAGGTTGCTGTTATTGTCCTACGGATTATATTTTTTCTG 6 0.15 No Hit AACAGCCCGCTGAGGCAGCTGGTGGAGGAAGACGAGGCGGCCAGGAAGCT 6 0.15 No Hit AGATCACAACCGTAGACTGGATATCTGAATGGATGACAGGGGAAATCAAT 6 0.15 No Hit AGGAGGATGGTAATAAGAGACCACATCGATCCAGATCTGACGTATATGGA 6 0.15 No Hit GGCGATGGGAACCTGGCCGTGCTGTGCTTCGGCGTCGGCGCGGAGCGCGA 6 0.15 No Hit GACACAGTGCTACGAGGATGCTAAGCTGATGAAGCTGTTCCCTGAAATTG 6 0.15 No Hit AGTTGTACTTGCAAATGCAGGTTGCTGTTATTGTCCTACGGATTATATTT 6 0.15 No Hit GATGGCCTCGGCCCCGAAGCAAACTATATTACTTTTTGAGTATAAATCCT 6 0.15 No Hit GGATGTTGTCTCAGAGGCTGACATCTTTGTGACCACCACTGGAAACAAGG 6 0.15 No Hit GGAGAGGTACCGGAGCTGGGCGCCGGATCGGCAGGACCGGTTTGTGAAGA 6 0.15 No Hit GATGGATCTGCCGATAGGCTGCATCTATTTAAAGCCGATTTATTAGAAGA 6 0.15 No Hit CCTTGATGTTGCTTTCAGTATTTCCCTATATCCCATGTCTGTTGAGGAAG 6 0.15 No Hit AGAAGGAGAACGATTTTCAGCAGTCTGGGGAGAGGTACCGGAGCTGGGCG 6 0.15 No Hit TGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGATGGC 6 0.15 No Hit GGTTGCCGTGGTCTGTGGTTATGGTGATGTTGGCAAGGGCTGTGCTTCTG 6 0.15 No Hit CTGGGTCAACTTTCTCTCCCAGTGTGATAAGTCGTGTGGGATGAAGGTTG 6 0.15 No Hit GGCGAACATCAAGGAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGG 6 0.15 No Hit GGTTCGCGGATGCTTTGGGGCATCCCAAGGTTAGCCATGAGCTCCGCGCT 6 0.15 No Hit CCCAGCCCTTCTTCTCAAAACTGAAAAGAAACACGCATGGAGATGGAGAA 6 0.15 No Hit CTAGAACTGGAACTTATATACAAGGTTCAGACACAGTGCTACGAGGATGC 6 0.15 No Hit CCTTGATGGAGGGTCTCCAGATCCTCACCTTGGAGGATGTTGTCTCAGAG 6 0.15 No Hit GTGGATATGCATCGACTGCCATGGATTGGATGGATGGATATGCTGTGGGA 6 0.15 No Hit TATGCATCGACTGCCATGGATTGGATGGATGGATATGCTGTGGGACAGTC 6 0.15 No Hit ACATCAAGGAGGGTTCAGTGATCGTGCTCCCCGCGGCGCACCCGGCGACG 6 0.15 No Hit CTTTCTCTCCCAGTGTGATAAGTCGTGTGGGATGAAGGTTGCGAACCGGC 6 0.15 No Hit GTGCTTCGGCGTCGGCGCGGAGCGCGACGAGAAGGTGTTTTTAACGGGGA 6 0.15 No Hit CTTTGGTTCCGTAAGGGTTCAAACCAGAAAGGAAGGCAATCTTTCGTGAA 6 0.15 No Hit GGCACTCTCCGCGACCCCGCTGATCCGAAGAAGATAGAACATTTACGTGC 6 0.15 No Hit AAGTTGTACTTGCAAATGCAGGTTGCTGTTATTGTCCTACGGATTATATT 6 0.15 No Hit GGATATGCATCGACTGCCATGGATTGGATGGATGGATATGCTGTGGGACA 6 0.15 No Hit AGCATGTGATCCATGAGAGTACAGTGGATATGCATCGACTGCCATGGATT 6 0.15 No Hit CTGTGGTTATGGTGATGTTGGCAAGGGCTGTGCTTCTGCGCTCAAGCAGG 6 0.15 No Hit CAGGCCTTGATGGAGGGTCTCCAGATCCTCACCTTGGAGGATGTTGTCTC 6 0.15 No Hit TGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGA 6 0.15 No Hit CCTTTCTACAATAATCCGAAAGATCCTCAGATTGAGTTGATTGACCCAGC 6 0.15 No Hit TGGATGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGC 6 0.15 No Hit CAACGTCAACGACTCCGTCACCAAGAGCAAGTTCGACAATCTGTATGGTT 5 0.125 No Hit TGGAGGAAGACGAGGCGGCCAGGAAGCTCGTGTTCGGGGAACGGGCCCAG 5 0.125 No Hit CAAAACTGAAAAGAAACACGCATGGAGATGGAGAAGACGGAGGAGAAGAA 5 0.125 No Hit CGATGGGAACCTGGCCGTGCTGTGCTTCGGCGTCGGCGCGGAGCGCGACG 5 0.125 No Hit GTGGGATGAAGGTTGCGAACCGGCTCAACGTGAAGCCGAGCATGTGATCC 5 0.125 No Hit CTTGAAGAGGCTGAGGAGGAAGAATAAATTGTGCTACCGAAGATCCCTTG 5 0.125 No Hit CAGTGTGATAAGTCGTGTGGGATGAAGGTTGCGAACCGGCTCAACGTGAA 5 0.125 No Hit GAACGATTTTCAGCAGTCTGGGGAGAGGTACCGGAGCTGGGCGCCGGATC 5 0.125 No Hit GTGGCGGGCGGCGATGGGAACCTGGCCGTGCTGTGCTTCGGCGTCGGCGC 5 0.125 No Hit CATGAGAGTACAGTGGATATGCATCGACTGCCATGGATTGGATGGATGGA 5 0.125 No Hit GGGCATCCCAAGGTTAGCCATGAGCTCCGCGCTATCTGGGTCAACTTTCT 5 0.125 No Hit GCTAGAACTGGAACTTATATACAAGGTTCAGACACAGTGCTACGAGGATG 5 0.125 No Hit ATGGATTGGATGGATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATG 5 0.125 No Hit CGCGACGAGAAGGTGTTTTTAACGGGGAAGAACAGCCCGCTGAGGCAGCT 5 0.125 No Hit AGGAAATATCGCGAAGAAGGCGCTCCGTGCCACTTGCGAAGGTTCTGGCC 5 0.125 No Hit TGAGAGTACAGTGGATATGCATCGACTGCCATGGATTGGATGGATGGATA 5 0.125 No Hit GGCAGGTGGGGGCGAACATCAAGGAGGGTTCAGTGATCGTGCTCCCCGCG 5 0.125 No Hit GGGAACCTGGCCGTGCTGTGCTTCGGCGTCGGCGCGGAGCGCGACGAGAA 5 0.125 No Hit AAAAGAAACACGCATGGAGATGGAGAAGACGGAGGAGAAGAAGAAGGTGG 5 0.125 No Hit TGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAG 5 0.125 No Hit GTAAGGGTTCAAACCAGAAAGGAAGGCAATCTTTCGTGAAAGCGCTGGAG 5 0.125 No Hit GGCAGCTGGTGGAGGAAGACGAGGCGGCCAGGAAGCTCGTGTTCGGGGAA 5 0.125 No Hit GGAAGAACAGCCCGCTGAGGCAGCTGGTGGAGGAAGACGAGGCGGCCAGG 5 0.125 No Hit GACCGGTTTGTGAAGAGGTTCGCGGATGCTTTGGGGCATCCCAAGGTTAG 5 0.125 No Hit GTTGGCAAGGGCTGTGCTTCTGCGCTCAAGCAGGCTGGTGCCCGTGTCAT 5 0.125 No Hit GAATAAATTGTGCTACCGAAGATCCCTTGATGTTGCTTTCAGTATTTCCC 5 0.125 No Hit GGAAATATCGCGAAGAAGGCGCTCCGTGCCACTTGCGAAGGTTCTGGCCG 5 0.125 No Hit CCTGGACCAGGAGGATGGTAATAAGAGACCACATCGATCCAGATCTGACG 5 0.125 No Hit AGGATCCTTTGATGCTGTAGTTGACGGTTGTGAATGCGTATTTCATACAG 5 0.125 No Hit ATCGACTGCCATGGATTGGATGGATGGATATGCTGTGGGACAGTCGGAAA 5 0.125 No Hit ATCAAGAAGGAGAACGATTTTCAGCAGTCTGGGGAGAGGTACCGGAGCTG 5 0.125 No Hit GGAGGAAGAATAAATTGTGCTACCGAAGATCCCTTGATGTTGCTTTCAGT 5 0.125 No Hit GCGCTATCTGGGTCAACTTTCTCTCCCAGTGTGATAAGTCGTGTGGGATG 5 0.125 No Hit GATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGACCAGGAGGATGGTAATA 5 0.125 No Hit CGACAATCTGTATGGTTGCCGCCACTCTCTCCCTGATGGTCTTATGAGGG 5 0.125 No Hit GGTTCAAACCAGAAAGGAAGGCAATCTTTCGTGAAAGCGCTGGAGCCTTT 5 0.125 No Hit AAGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTGGACC 5 0.125 No Hit ACCATACTTCTTTGGTTCCGTAAGGGTTCAAACCAGAAAGGAAGGCAATC 5 0.125 No Hit GGTTTGTGAAGAGGTTCGCGGATGCTTTGGGGCATCCCAAGGTTAGCCAT 5 0.125 No Hit GCAGGCTGGTGCCCGTGTCATTGTTACTGAGATTGATCCCATCTGTGCCC 5 0.125 No Hit AGGAGGAAGAATAAATTGTGCTACCGAAGATCCCTTGATGTTGCTTTCAG 5 0.125 No Hit ATGGATATGCTGTGGGACAGTCGGAAATAAAAGTATGTTAAGCTGGATGG 5 0.125 No Hit CCTCCTCTTCCCTGCCATCAACGTCAACGACTCCGTCACCAAGAGCAAGT 5 0.125 No Hit GCCTTTTGTCAAATGGCTTGAAGAGGCTGAGGAGGAAGAATAAATTGTGC 5 0.125 No Hit ATAAAAGTATGTTAAGCTGGATGGCCGTAAGCTTTGTGCTCTTCTCCCTG 5 0.125 No Hit AAGAGGCTCTACCAGATGCAGGAATCCGGCACCCTCCTCTTCCCTGCCAT 5 0.125 No Hit GGTTATGGTGATGTTGGCAAGGGCTGTGCTTCTGCGCTCAAGCAGGCTGG 5 0.125 No Hit AGGAGTTCGAGAAGTCCGGCAAGATCCCCGACCCGGAGTCCACCGACAAC 5 0.125 No Hit AAATTGTGCTACCGAAGATCCCTTGATGTTGCTTTCAGTATTTCCCTATA 5 0.125 No Hit ATGTTGGCAAGGGCTGTGCTTCTGCGCTCAAGCAGGCTGGTGCCCGTGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAACAA 35 0.0029932235 21.312336 125-129 >>END_MODULE Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327252 spots for ERR5262780.sra Written 1327252 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra Read 1327240 spots for ERR5262780.sra Written 1327240 spots for ERR5262780.sra SRR ids: ['ERR5262780.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wlxtqo41 ERR5262780.sra spots: 26544812 blocks: [[1, 1327240], [1327241, 2654480], [2654481, 3981720], [3981721, 5308960], [5308961, 6636200], [6636201, 7963440], [7963441, 9290680], [9290681, 10617920], [10617921, 11945160], [11945161, 13272400], [13272401, 14599640], [14599641, 15926880], [15926881, 17254120], [17254121, 18581360], [18581361, 19908600], [19908601, 21235840], [21235841, 22563080], [22563081, 23890320], [23890321, 25217560], [25217561, 26544812]] ERR5262780 file size 8682336 ERR5262780 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262780 ERR5262780_1.fastq ERR5262780_2.fastq Input file: ERR5262780_1.fastq Paired file: ERR5262780_2.fastq trimmed: ERR5262780-trimmed-pair1.fastq, ERR5262780-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:13:34 2024 >> started Fri Dec 6 11:14:03 2024 >> done (28.455s) 26544812 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 26544812 (100.00%) read pairs available; of these: 8147 ( 0.03%) trimmed read pairs available after processing 26536665 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 26 4 0.00% 27 0 0.00% 28 1 0.00% 29 3 0.00% 30 3 0.00% 31 0 0.00% 32 0 0.00% 33 2 0.00% 34 1 0.00% 35 0 0.00% 36 2 0.00% 37 2 0.00% 38 4 0.00% 39 2 0.00% 40 0 0.00% 41 2 0.00% 42 1 0.00% 43 0 0.00% 44 1 0.00% 45 3 0.00% 46 1 0.00% 47 4 0.00% 48 1 0.00% 49 332 0.00% 50 355 0.00% 51 416 0.00% 52 430 0.00% 53 424 0.00% 54 468 0.00% 55 491 0.00% 56 548 0.00% 57 710 0.00% 58 810 0.00% 59 909 0.00% 60 1006 0.00% 61 1112 0.00% 62 1207 0.00% 63 1265 0.00% 64 1483 0.01% 65 1678 0.01% 66 1836 0.01% 67 2058 0.01% 68 2334 0.01% 69 2699 0.01% 70 3019 0.01% 71 3585 0.01% 72 3979 0.01% 73 4645 0.02% 74 4866 0.02% 75 5341 0.02% 76 5989 0.02% 77 6591 0.02% 78 7380 0.03% 79 8254 0.03% 80 9220 0.03% 81 10306 0.04% 82 11476 0.04% 83 12958 0.05% 84 14332 0.05% 85 15890 0.06% 86 16268 0.06% 87 18115 0.07% 88 19449 0.07% 89 20032 0.08% 90 22347 0.08% 91 24441 0.09% 92 25705 0.10% 93 28448 0.11% 94 30602 0.12% 95 32003 0.12% 96 33778 0.13% 97 35250 0.13% 98 36627 0.14% 99 38070 0.14% 100 40818 0.15% 101 42190 0.16% 102 44889 0.17% 103 47546 0.18% 104 48906 0.18% 105 52002 0.20% 106 53897 0.20% 107 54510 0.21% 108 56484 0.21% 109 57993 0.22% 110 59482 0.22% 111 61191 0.23% 112 64604 0.24% 113 66031 0.25% 114 69135 0.26% 115 71101 0.27% 116 71865 0.27% 117 74533 0.28% 118 75724 0.29% 119 76356 0.29% 120 76313 0.29% 121 79337 0.30% 122 80354 0.30% 123 83028 0.31% 124 86745 0.33% 125 86989 0.33% 126 88786 0.33% 127 89645 0.34% 128 89980 0.34% 129 92282 0.35% 130 93122 0.35% 131 93148 0.35% 132 94969 0.36% 133 97978 0.37% 134 98330 0.37% 135 100045 0.38% 136 101822 0.38% 137 101310 0.38% 138 102935 0.39% 139 104672 0.39% 140 105274 0.40% 141 108202 0.41% 142 110257 0.42% 143 111572 0.42% 144 114420 0.43% 145 113807 0.43% 146 116022 0.44% 147 228484 0.86% 148 107442 0.40% 149 107428 0.40% 150 21759283 81.97% 26544812 reads passed initial QC criterion=sequence-density sequence-density=1.22 sequence-density-rank=1 fanout-score=5.71 fanout-score-rank=19 prefix-density=6.98 prefix-fanout=1.0 sequence=GTCTCCCTGTCCTGGATGATCTTGGCGTCGAGGATCTCGCCGA criterion=fanout-score sequence-density=0.04 sequence-density-rank=22 fanout-score=118.09 fanout-score-rank=1 prefix-density=0.90 prefix-fanout=5.0 sequence=CCGCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=8.12 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=37 prefix-density=8.09 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.35 sequence-density-rank=11 fanout-score=51.47 fanout-score-rank=1 prefix-density=8.43 prefix-fanout=2.2 sequence=GTTCCGGTTCGCGGCTAGCAGTAGTTGTTGTAGTAGCAGCTAGGGTTTCCGGTAGGGTTCCGTCGAGATCGCCATGGATGAGTACCGCTGCTTCGTGGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTCTCCCTGTCCTGGATGATCTTGGCGTCGAGGATCTCGCCGA -y CGGTTCCGGTTC -o ERR5262780 ERR5262780_1.fastq ERR5262780_2.fastq Input file: ERR5262780_1.fastq Paired file: ERR5262780_2.fastq trimmed: ERR5262780-trimmed-pair1.fastq, ERR5262780-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTCTCCCTGTCCTGGATGATCTTGGCGTCGAGGATCTCGCCGA -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:18:34 2024 >> started Fri Dec 6 11:18:53 2024 >> done (19.018s) 17696541 read pairs processed; of these: 58 ( 0.00%) short read pairs filtered out after trimming by size control 268 ( 0.00%) empty read pairs filtered out after trimming by size control 17696215 (100.00%) read pairs available; of these: 137 ( 0.00%) trimmed read pairs available after processing 17696078 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 26 1 0.00% 27 0 0.00% 28 1 0.00% 29 2 0.00% 30 3 0.00% 31 0 0.00% 32 0 0.00% 33 2 0.00% 34 1 0.00% 35 0 0.00% 36 1 0.00% 37 1 0.00% 38 2 0.00% 39 1 0.00% 40 0 0.00% 41 1 0.00% 42 0 0.00% 43 0 0.00% 44 0 0.00% 45 2 0.00% 46 1 0.00% 47 2 0.00% 48 1 0.00% 49 150 0.00% 50 153 0.00% 51 189 0.00% 52 192 0.00% 53 206 0.00% 54 348 0.00% 55 426 0.00% 56 401 0.00% 57 514 0.00% 58 612 0.00% 59 639 0.00% 60 736 0.00% 61 875 0.00% 62 742 0.00% 63 214 0.00% 64 641 0.00% 65 1273 0.01% 66 1475 0.01% 67 1015 0.01% 68 868 0.00% 69 2232 0.01% 70 2480 0.01% 71 1430 0.01% 72 3311 0.02% 73 2227 0.01% 74 3335 0.02% 75 3754 0.02% 76 4018 0.02% 77 4595 0.03% 78 4803 0.03% 79 4945 0.03% 80 6528 0.04% 81 8199 0.05% 82 6391 0.04% 83 10884 0.06% 84 7915 0.04% 85 11054 0.06% 86 10515 0.06% 87 12752 0.07% 88 12543 0.07% 89 13988 0.08% 90 14382 0.08% 91 15059 0.09% 92 16803 0.09% 93 20559 0.12% 94 20148 0.11% 95 20917 0.12% 96 22739 0.13% 97 22408 0.13% 98 23610 0.13% 99 26487 0.15% 100 26741 0.15% 101 28589 0.16% 102 30268 0.17% 103 31480 0.18% 104 32268 0.18% 105 34683 0.20% 106 34818 0.20% 107 35474 0.20% 108 37836 0.21% 109 37314 0.21% 110 39606 0.22% 111 40743 0.23% 112 43762 0.25% 113 44138 0.25% 114 45849 0.26% 115 48098 0.27% 116 47635 0.27% 117 49349 0.28% 118 50656 0.29% 119 50565 0.29% 120 50560 0.29% 121 52951 0.30% 122 53162 0.30% 123 55118 0.31% 124 57956 0.33% 125 58029 0.33% 126 58940 0.33% 127 59773 0.34% 128 59845 0.34% 129 61634 0.35% 130 62190 0.35% 131 62247 0.35% 132 63181 0.36% 133 64891 0.37% 134 65222 0.37% 135 66635 0.38% 136 67616 0.38% 137 66867 0.38% 138 68197 0.39% 139 69737 0.39% 140 69853 0.39% 141 71952 0.41% 142 73222 0.41% 143 73989 0.42% 144 76247 0.43% 145 75558 0.43% 146 77469 0.44% 147 150346 0.85% 148 71779 0.41% 149 71759 0.41% 150 14516716 82.03% criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=5.59 fanout-score-rank=30 prefix-density=0.40 prefix-fanout=4.0 sequence=TGCAGTTGTCGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=40 fanout-score=409.91 fanout-score-rank=1 prefix-density=0.62 prefix-fanout=21.6 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCT criterion=sequence-density sequence-density=0.89 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=36 prefix-density=0.92 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=21 fanout-score=163.77 fanout-score-rank=1 prefix-density=0.94 prefix-fanout=19.6 sequence=CGCCGCCGCCGG ERR5262780 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:20:01 Started mapping on | Dec 06 11:20:01 Finished on | Dec 06 11:22:21 Mapping speed, Million of reads per hour | 682.57 Number of input reads | 26544486 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 25562504 Uniquely mapped reads % | 96.30% Average mapped length | 289.76 Number of splices: Total | 24324967 Number of splices: Annotated (sjdb) | 22604957 Number of splices: GT/AG | 23963607 Number of splices: GC/AG | 302233 Number of splices: AT/AC | 15003 Number of splices: Non-canonical | 44124 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.21 Insertion rate per base | 0.01% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 303963 % of reads mapped to multiple loci | 1.15% Number of reads mapped to too many loci | 775 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.54% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 678019 678019 678019 N_multimapping 303963 303963 303963 N_noFeature 1086096 24875553 1321047 N_ambiguous 535436 3304 83933 UnstrandedReadsAssigned:23940972 PositiveStrandReadsAssigned:683647 NegativeStrandReadsAssigned:24157524 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262780 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262780-trimmed-pair1.fastq ERR5262780-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,544,486 reads, 24,487,695 reads pseudoaligned [quant] estimated average fragment length: 256.562 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,171 rounds 52973 ERR5262780.ke.tsv 35125 ERR5262780.se.tsv 88098 total ==> ERR5262780.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 680.966 0 0 PNS24247 1044 788.438 126.669 9.41568 PNS24249 1928 1672.44 366.532 12.8443 PNS24246 1044 788.438 126.669 9.41568 PNS24248 1044 788.438 126.669 9.41568 PNS24244 1471 1215.44 143.462 6.91757 PNS24243 293 107.866 1 0.543332 KQK14069 1603 1347.44 53515.3 2327.66 KQK14071 474 246.123 1008.83 240.224 ==> ERR5262780.se.tsv <== BRADI_1g14170v3 57526 BRADI_1g53295v3 307 BRADI_1g59795v3 922 BRADI_1g07683v3 0 BRADI_1g00485v3 21 BRADI_1g20270v3 1050 BRADI_1g74790v3 2041 BRADI_1g09890v3 0 BRADI_1g77505v3 339 BRADI_1g48960v3 0 ERR5262780 completed mapping pipeline successfully