Starting /dee2/code/volunteer_pipeline.sh ERR5262781 current disk space = 1551566372864 free memory = 1596021444 ERR5262781 SRAfilesize 1c43dc2c0652ccefbb2f642015c096f3 ERR5262781.sra ERR5262781.sra file validated ERR5262781 is paired end ERR5262781 is conventional basespace ERR5262781 read1 length is 67-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262781_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 67-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6155 37.0 37.0 37.0 37.0 37.0 2 36.45325 37.0 37.0 37.0 37.0 37.0 3 36.6155 37.0 37.0 37.0 37.0 37.0 4 36.714 37.0 37.0 37.0 37.0 37.0 5 36.6845 37.0 37.0 37.0 37.0 37.0 6 36.6585 37.0 37.0 37.0 37.0 37.0 7 36.6345 37.0 37.0 37.0 37.0 37.0 8 36.7185 37.0 37.0 37.0 37.0 37.0 9 36.6635 37.0 37.0 37.0 37.0 37.0 10-14 36.675 37.0 37.0 37.0 37.0 37.0 15-19 36.58 37.0 37.0 37.0 37.0 37.0 20-24 36.53789999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.556200000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.541000000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.5184 37.0 37.0 37.0 37.0 37.0 40-44 36.4446 37.0 37.0 37.0 37.0 37.0 45-49 36.4478 37.0 37.0 37.0 37.0 37.0 50-54 36.406600000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.3612 37.0 37.0 37.0 37.0 37.0 60-64 36.343900000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.294023005751434 37.0 37.0 37.0 37.0 37.0 70-74 36.27720982176073 37.0 37.0 37.0 37.0 37.0 75-79 36.237647058823526 37.0 37.0 37.0 37.0 37.0 80-84 36.309037718272954 37.0 37.0 37.0 37.0 37.0 85-89 36.2075632158818 37.0 37.0 37.0 37.0 37.0 90-94 36.08915947863068 37.0 37.0 37.0 37.0 37.0 95-99 36.13321273475944 37.0 37.0 37.0 37.0 37.0 100-104 36.12838649665613 37.0 37.0 37.0 37.0 37.0 105-109 36.135419176362554 37.0 37.0 37.0 37.0 37.0 110-114 36.104574294427245 37.0 37.0 37.0 37.0 37.0 115-119 36.13215868870725 37.0 37.0 37.0 37.0 37.0 120-124 36.056145906791635 37.0 37.0 37.0 37.0 37.0 125-129 36.04164609879053 37.0 37.0 37.0 37.0 37.0 130-134 35.9503558789342 37.0 37.0 37.0 37.0 37.0 135-139 35.982286646744996 37.0 37.0 37.0 37.0 37.0 140-144 35.96191791309283 37.0 37.0 37.0 37.0 37.0 145-149 35.84976158971237 37.0 37.0 37.0 37.0 37.0 150 35.928255093002655 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 1.0 23 2.0 24 1.0 25 2.0 26 7.0 27 6.0 28 9.0 29 9.0 30 10.0 31 36.0 32 41.0 33 62.0 34 124.0 35 310.0 36 2986.0 37 390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.375 12.125 9.1 31.4 2 23.58089522380595 11.10277569392348 36.25906476619154 29.057264316079017 3 17.7 19.5 31.85 30.95 4 19.925 23.275000000000002 29.099999999999998 27.700000000000003 5 19.125 33.35 27.900000000000002 19.625 6 21.025 37.35 26.724999999999998 14.899999999999999 7 14.899999999999999 24.15 41.8 19.15 8 12.975 24.224999999999998 33.550000000000004 29.25 9 14.35 21.8 38.3 25.55 10-14 18.145 28.22 28.084999999999997 25.55 15-19 18.96 28.965000000000003 26.275 25.8 20-24 20.585 29.89 24.83 24.695 25-29 19.18 31.25 25.974999999999998 23.595 30-34 19.895 27.525 26.064999999999998 26.515 35-39 22.040000000000003 31.269999999999996 24.075 22.615 40-44 19.205 33.79 24.87 22.134999999999998 45-49 17.805 31.14 26.06 24.995 50-54 23.465 29.959999999999997 26.035000000000004 20.54 55-59 21.275 33.910000000000004 23.73 21.085 60-64 18.345 35.85 23.169999999999998 22.634999999999998 65-69 18.146814681468147 32.408240824082405 27.27272727272727 22.172217221722175 70-74 21.295230468945498 34.097392522896754 24.378159251288725 20.229217756869026 75-79 20.811013767209012 35.77972465581977 21.987484355444305 21.42177722152691 80-84 18.773504487790202 29.102943388657675 28.0750137892995 24.04853833425262 85-89 20.80216856583505 33.39691782541037 23.723708649164198 22.077204959590382 90-94 20.952333065164925 33.4473049074819 22.9635961383749 22.636765888978278 95-99 19.170697299476018 33.60036275695284 26.73317210802096 20.495767835550183 100-104 19.47086199919061 31.900040469445567 28.11614730878187 20.51295022258195 105-109 21.4263835001021 32.78537880334899 24.127016540739227 21.661221155809677 110-114 20.103092783505154 32.92783505154639 29.09278350515464 17.876288659793815 115-119 20.625391522238463 29.139695134683652 27.82940070996033 22.40551263311756 120-124 21.202848852545504 29.227116855710893 25.713532049591137 23.856502242152466 125-129 22.33974358974359 29.364316239316242 27.92735042735043 20.368589743589745 130-134 21.483126591192242 30.778397703266346 27.842478738963223 19.895996966578192 135-139 21.42185858809221 28.56984907955111 27.265188788766654 22.743103543590028 140-144 20.263591433278417 26.387547577117537 27.091972959154692 26.256888030449353 145-149 21.43565222461951 27.768382996093067 28.15324508717709 22.642719692110326 150 19.66341895482728 27.989371124889285 27.723649247121347 24.62356067316209 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 0.0 20 0.5 21 1.0 22 1.0 23 0.5 24 0.0 25 1.0 26 4.0 27 6.5 28 5.0 29 4.0 30 13.0 31 29.0 32 55.5 33 75.0 34 102.0 35 103.0 36 80.5 37 168.0 38 299.5 39 241.0 40 188.0 41 222.0 42 214.5 43 231.0 44 248.0 45 250.0 46 237.0 47 183.5 48 135.0 49 146.0 50 150.0 51 88.0 52 34.5 53 41.0 54 68.5 55 182.0 56 155.0 57 19.0 58 3.0 59 4.5 60 3.0 61 1.5 62 0.0 63 0.0 64 0.5 65 3.0 66 3.5 67 1.5 68 0.5 69 0.0 70 1.5 71 1.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 0.0 70-71 3.0 72-73 1.0 74-75 0.0 76-77 0.0 78-79 3.0 80-81 3.0 82-83 3.0 84-85 1.0 86-87 2.0 88-89 1.0 90-91 3.0 92-93 6.0 94-95 2.0 96-97 2.0 98-99 10.0 100-101 5.0 102-103 7.0 104-105 21.0 106-107 15.0 108-109 17.0 110-111 10.0 112-113 21.0 114-115 22.0 116-117 20.0 118-119 13.0 120-121 15.0 122-123 23.0 124-125 15.0 126-127 21.0 128-129 18.0 130-131 24.0 132-133 26.0 134-135 20.0 136-137 41.0 138-139 55.0 140-141 29.0 142-143 33.0 144-145 50.0 146-147 26.0 148-149 25.0 150-151 3387.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.025 #Duplication Level Percentage of deduplicated Percentage of total 1 45.235892691951896 12.225 2 17.668825161887142 9.55 3 11.100832562442182 9.0 4 6.475485661424607 7.000000000000001 5 4.25531914893617 5.75 6 3.0527289546716005 4.95 7 2.127659574468085 4.025 8 1.6651248843663276 3.5999999999999996 9 1.572617946345976 3.8249999999999997 >10 6.567992599444958 33.85 >50 0.18501387604070307 3.55 >100 0.09250693802035154 2.675 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAG 107 2.675 No Hit GGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAG 71 1.775 No Hit GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 71 1.775 No Hit GTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAACATGCACG 44 1.0999999999999999 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 44 1.0999999999999999 No Hit GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 43 1.075 No Hit GTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 41 1.0250000000000001 No Hit GCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATG 38 0.95 No Hit TAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAG 36 0.8999999999999999 No Hit CTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGA 34 0.8500000000000001 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 33 0.8250000000000001 No Hit GGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAG 33 0.8250000000000001 No Hit GCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACA 33 0.8250000000000001 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 31 0.775 No Hit AGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 31 0.775 No Hit AGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCA 30 0.75 No Hit CCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAAC 29 0.7250000000000001 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 28 0.7000000000000001 No Hit ATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTAT 28 0.7000000000000001 No Hit CTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCAT 27 0.675 No Hit GGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAAT 26 0.65 No Hit CCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCC 24 0.6 No Hit GTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAG 20 0.5 No Hit CCAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGC 19 0.475 No Hit GGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTCAG 19 0.475 No Hit TTTTTTTTGAAAATAACGATGCCGCCGCACCACAGAACAACATTCCACAT 19 0.475 No Hit GACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCC 19 0.475 No Hit GTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCA 19 0.475 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 18 0.44999999999999996 No Hit GTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATA 18 0.44999999999999996 No Hit GGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATC 18 0.44999999999999996 No Hit CACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCA 18 0.44999999999999996 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 18 0.44999999999999996 No Hit CATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGC 18 0.44999999999999996 No Hit GTAAGACTTTTTGAATTCCAGAAAGAGCCATACGGCTAATTGATATCCAT 17 0.42500000000000004 No Hit TGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTG 17 0.42500000000000004 No Hit CTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACC 17 0.42500000000000004 No Hit CTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGC 16 0.4 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 16 0.4 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 15 0.375 No Hit GGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGG 15 0.375 No Hit GTCCAGCTTCTCCAGTTCGCCCATCCTTTCTATGATTGCCTTTTTGCTTG 15 0.375 No Hit GTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCAT 14 0.35000000000000003 No Hit GCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGA 14 0.35000000000000003 No Hit CTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATG 14 0.35000000000000003 No Hit GGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAA 14 0.35000000000000003 No Hit TTTTTTTTTTTGAAAATAACGATGCCGCCGCACCACAGAACAACATTCCA 14 0.35000000000000003 No Hit CGGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTG 14 0.35000000000000003 No Hit GGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGC 13 0.325 No Hit GTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCA 13 0.325 No Hit CTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACA 12 0.3 No Hit GGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCA 12 0.3 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 12 0.3 No Hit CCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGG 12 0.3 No Hit GCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATAT 12 0.3 No Hit GCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAGA 11 0.27499999999999997 No Hit GGTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCA 11 0.27499999999999997 No Hit TGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCACTTAACTAC 11 0.27499999999999997 No Hit ACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAG 11 0.27499999999999997 No Hit TTTTTTTTTGAAAATAACGATGCCGCCGCACCACAGAACAACATTCCACA 11 0.27499999999999997 No Hit GTTGAATAAAAAAAACTACTGGAAGAGACGTAAATTCTGCCAGCTCAAAA 11 0.27499999999999997 No Hit CTCCTCTTCTAGTATGAACTGCGGAAGCTGTGTTGTTTTACCACAACCTG 11 0.27499999999999997 No Hit CTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTT 11 0.27499999999999997 No Hit CCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 11 0.27499999999999997 No Hit CCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATAC 11 0.27499999999999997 No Hit CCGGAATGCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGC 10 0.25 No Hit CGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCT 10 0.25 No Hit GGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTATCCGGTGAT 10 0.25 No Hit CAGTGACTGACTCCTTACATAAATTAACTTAATTGAAAAATGACAAAGTA 10 0.25 No Hit CCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCA 10 0.25 No Hit GCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGA 10 0.25 No Hit CTGTAAGACTTTTTGAATTCCAGAAAGAGCCATACGGCTAATTGATATCC 10 0.25 No Hit CAGGAAATTCATCCTTCAGCAAAGAAAGAAAAATAGGAAGAAGTTGTTCA 10 0.25 No Hit CCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAA 10 0.25 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTT 9 0.22499999999999998 No Hit CTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAG 9 0.22499999999999998 No Hit CAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGA 9 0.22499999999999998 No Hit ATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCT 9 0.22499999999999998 No Hit CTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAA 9 0.22499999999999998 No Hit CTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCG 9 0.22499999999999998 No Hit ATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGG 9 0.22499999999999998 No Hit CATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACG 9 0.22499999999999998 No Hit GCTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCT 9 0.22499999999999998 No Hit GTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACAAC 9 0.22499999999999998 No Hit GCATGATGTTGAAAACCAACGGGCATCCTACAGTCTACGGAATAATCACA 9 0.22499999999999998 No Hit GCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAG 9 0.22499999999999998 No Hit GTCGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCC 9 0.22499999999999998 No Hit CCAGAAAGTAATCCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGG 9 0.22499999999999998 No Hit GTGCATGCTTTCAAGAAATTAGGATTACATGTTGAATAAAAAAAACTACT 9 0.22499999999999998 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 9 0.22499999999999998 No Hit GTGACTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTTAATT 9 0.22499999999999998 No Hit AGTATTTTCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACT 8 0.2 No Hit ATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCC 8 0.2 No Hit GTACGCGGCGAGTGTGAGTTCTGCTTACCATTGGCAGGGGAAGGGAGAGA 8 0.2 No Hit CTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCACTTAACTA 8 0.2 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACA 8 0.2 No Hit GTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCA 8 0.2 No Hit CTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACT 8 0.2 No Hit ACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATA 8 0.2 No Hit GGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACC 8 0.2 No Hit GTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAA 8 0.2 No Hit GACACATCTGGTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATG 8 0.2 No Hit CCCTGATGTGTTGAGTCAACAACTCCTCCTTGAGCCACCTCATCTAACAG 8 0.2 No Hit GGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTC 8 0.2 No Hit GCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATA 8 0.2 No Hit TGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAG 8 0.2 No Hit AGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 8 0.2 No Hit TTTTTTTTTTTTGAAAATAACGATGCCGCCGCACCACAGAACAACATTCC 8 0.2 No Hit CACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCAT 8 0.2 No Hit CACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCT 7 0.17500000000000002 No Hit GGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTT 7 0.17500000000000002 No Hit GGTGACTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTTAAT 7 0.17500000000000002 No Hit GCTCCTAGGAGGTCACACAAGTTAGATCGGATCGTCTCCTTGAATGGGCT 7 0.17500000000000002 No Hit AGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCAT 7 0.17500000000000002 No Hit AGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAG 7 0.17500000000000002 No Hit GCTTTCAAGAAATTAGGATTACATGTTGAATAAAAAAAACTACTGGAAGA 7 0.17500000000000002 No Hit AGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAAT 7 0.17500000000000002 No Hit GTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGGGTAA 7 0.17500000000000002 No Hit CCTAGACGTAATTTCCAAATGAGGGCTCCTGCTGGTCCCGTCTCCCCCAC 7 0.17500000000000002 No Hit CATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTA 7 0.17500000000000002 No Hit CGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGT 7 0.17500000000000002 No Hit GAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCA 7 0.17500000000000002 No Hit GCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGT 7 0.17500000000000002 No Hit CACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCG 7 0.17500000000000002 No Hit TGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCA 7 0.17500000000000002 No Hit CTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 7 0.17500000000000002 No Hit ATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGCTT 7 0.17500000000000002 No Hit CTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACAACTAT 7 0.17500000000000002 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 7 0.17500000000000002 No Hit CACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAA 7 0.17500000000000002 No Hit CCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGG 7 0.17500000000000002 No Hit CCACAACTATCAATATACCTGGAATAAAAAAAAGAGAAATGGTTATGCTC 7 0.17500000000000002 No Hit CAACGGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATAT 6 0.15 No Hit ATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGG 6 0.15 No Hit GCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTC 6 0.15 No Hit GGGGTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTCCA 6 0.15 No Hit CCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCACTTAAC 6 0.15 No Hit GGCTGTCCTGTCCCCCCAGCCACACATGAGGTCATAGTACGAACACGGAG 6 0.15 No Hit GGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAAT 6 0.15 No Hit CTGTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATT 6 0.15 No Hit CCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTT 6 0.15 No Hit GGCGAGTGTGAGTTCTGCTTACCATTGGCAGGGGAAGGGAGAGAATGGCG 6 0.15 No Hit CAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTC 6 0.15 No Hit GTTTATGTTTGGTGGGCATAAATGCATGATCATTTCTTGGTTCTTGCAGA 6 0.15 No Hit CTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACC 6 0.15 No Hit AATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGA 6 0.15 No Hit GTTCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAG 6 0.15 No Hit CAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCA 6 0.15 No Hit GTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCT 6 0.15 No Hit AATGTAATCAGGTATCTTCCCAGTAAACTCGTTATCTGATGCCCATAGTA 6 0.15 No Hit CTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCCT 6 0.15 No Hit GTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGC 6 0.15 No Hit CTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAGAAGTAC 6 0.15 No Hit GCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTT 6 0.15 No Hit CCGGGGTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTC 6 0.15 No Hit ATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGA 6 0.15 No Hit CCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACAC 6 0.15 No Hit GCCAAACAAATAGGTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGG 6 0.15 No Hit GCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAAC 6 0.15 No Hit GCATGCTTTCAAGAAATTAGGATTACATGTTGAATAAAAAAAACTACTGG 6 0.15 No Hit GGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAA 6 0.15 No Hit GTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACA 6 0.15 No Hit GATTTTTTGAGAACGATGCTGCCCTCATCAACCCTGTCATAAAACCCTTC 6 0.15 No Hit CTGTAAAACGATGAATGAAAAGGCTGGGATCTATGAGCTTCTGAACAATT 6 0.15 No Hit ATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGC 6 0.15 No Hit GCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTT 5 0.125 No Hit GGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACAA 5 0.125 No Hit CGGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGA 5 0.125 No Hit CCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTAC 5 0.125 No Hit CAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCC 5 0.125 No Hit AGGAGCTTCCATTGAAATATGCAAAAGAACATGTGCATGCTTTCAAGAAA 5 0.125 No Hit GCCACACATGAGATCATAGTACGAACACGGAGAGAGGGTAAAACGAAGGG 5 0.125 No Hit CTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGA 5 0.125 No Hit ACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAAC 5 0.125 No Hit GGTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTCCACA 5 0.125 No Hit TGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTATCCGGTGA 5 0.125 No Hit GCAGTGACTTCAATGATTATTTCTATCCTAGTTGTGGAGTTGAGTATTCA 5 0.125 No Hit GCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAG 5 0.125 No Hit GCCTATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 5 0.125 No Hit TTTTTTTTTTGAAAATAACGATGCCGCCGCACCACAGAACAACATTCCAC 5 0.125 No Hit CCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAG 5 0.125 No Hit GTAGAGAAAACGACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCT 5 0.125 No Hit CCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGA 5 0.125 No Hit TCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTAC 5 0.125 No Hit TACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTCCACAAA 5 0.125 No Hit CAGTATTTTCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCAC 5 0.125 No Hit GGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTG 5 0.125 No Hit GGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATA 5 0.125 No Hit GTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGC 5 0.125 No Hit CTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACA 5 0.125 No Hit CGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACA 5 0.125 No Hit CTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTT 5 0.125 No Hit CGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTC 5 0.125 No Hit CTGCACTTCTGACTCTTTATCCTGTATTTTTCTGAGGATCTTATCTTCAA 5 0.125 No Hit CGGCGCTTTTTCTGCTCCATGATCTTTCAGAAAGCCTTCAGCTCACAGGT 5 0.125 No Hit CCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCAC 5 0.125 No Hit GTGAGTTCTGCTTACCATTGGCAGGGGAAGGGAGAGAATGGCGACGTGTG 5 0.125 No Hit CAGGGGATCCGGGTCGCTCTAGGAGAGATTGGGAATGGAGGGGCGGCGGA 5 0.125 No Hit ATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCTTTGG 5 0.125 No Hit GCATTATATTCGAAAGAACTTTTTTCATTGGCATCATTTTGTATGAACTA 5 0.125 No Hit GGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGT 5 0.125 No Hit GGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATA 5 0.125 No Hit CCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGGGTAAAACGA 5 0.125 No Hit AATTTTTTGAACCAAATGCGCAGAGTTCTCGTGTTCAGAAGCTGCAGTAT 5 0.125 No Hit CCTCCGTATCCTCCCAGCTGCTCCTTCTGGAATTGCAAAGGGGTCCTCAG 5 0.125 No Hit CTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAG 5 0.125 No Hit GCCAGCTCAAAATTTTTTCTTCCAGATATTCTGTAGAAGTTTGAAATGAC 5 0.125 No Hit CTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCCCAT 5 0.125 No Hit CCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAG 5 0.125 No Hit TTTTTTTTTTTTTGAAAATAACGATGCCGCCGCACCACAGAACAACATTC 5 0.125 No Hit GTAGATCTTTGACTTCGAGCTCATCACCCAGCCATTTTGGACAAGAAGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGTCC 35 0.0037465966 60.241074 3 CCCCCCA 35 0.0037465966 60.241074 8 CCCCCAG 35 0.0037465966 60.241074 9 CTGTCCC 35 0.0037465966 60.241074 4 TCCCCCC 35 0.0037465966 60.241074 7 GTCCCCC 40 0.006357104 52.710938 6 >>END_MODULE ERR5262781 read2 length is 67-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262781_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 67-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.055 37.0 37.0 37.0 37.0 37.0 2 35.977 37.0 37.0 37.0 37.0 37.0 3 35.977 37.0 37.0 37.0 37.0 37.0 4 36.107 37.0 37.0 37.0 37.0 37.0 5 36.1895 37.0 37.0 37.0 37.0 37.0 6 36.13 37.0 37.0 37.0 37.0 37.0 7 36.034 37.0 37.0 37.0 37.0 37.0 8 36.077 37.0 37.0 37.0 37.0 37.0 9 36.1265 37.0 37.0 37.0 37.0 37.0 10-14 36.2033 37.0 37.0 37.0 37.0 37.0 15-19 36.1632 37.0 37.0 37.0 37.0 37.0 20-24 36.1298 37.0 37.0 37.0 37.0 37.0 25-29 36.1557 37.0 37.0 37.0 37.0 37.0 30-34 36.1015 37.0 37.0 37.0 37.0 37.0 35-39 36.0895 37.0 37.0 37.0 37.0 37.0 40-44 35.99400000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.0004 37.0 37.0 37.0 37.0 37.0 50-54 35.975 37.0 37.0 37.0 37.0 37.0 55-59 35.935500000000005 37.0 37.0 37.0 37.0 37.0 60-64 35.858799999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.83337591897974 37.0 37.0 37.0 37.0 37.0 70-74 35.877944681083584 37.0 37.0 37.0 37.0 37.0 75-79 35.850062578222776 37.0 37.0 37.0 37.0 37.0 80-84 35.752020530466964 37.0 37.0 37.0 37.0 37.0 85-89 35.72405320819109 37.0 37.0 37.0 37.0 37.0 90-94 35.67434480055593 37.0 37.0 37.0 37.0 37.0 95-99 35.671177168819234 37.0 37.0 37.0 37.0 37.0 100-104 35.70734108409058 37.0 37.0 37.0 37.0 37.0 105-109 35.61078864393885 37.0 37.0 37.0 37.0 37.0 110-114 35.61439201682455 37.0 37.0 37.0 37.0 37.0 115-119 35.56187623440759 37.0 37.0 37.0 37.0 37.0 120-124 35.543465966074805 37.0 37.0 37.0 37.0 37.0 125-129 35.426055675615984 37.0 37.0 37.0 37.0 37.0 130-134 35.416272067610755 37.0 37.0 37.0 37.0 37.0 135-139 35.374636939901244 37.0 37.0 37.0 34.6 37.0 140-144 35.25383035345877 37.0 37.0 37.0 29.8 37.0 145-149 35.21102176752764 37.0 37.0 37.0 29.8 37.0 150 35.111924992675064 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 2.0 13 1.0 14 1.0 15 1.0 16 1.0 17 0.0 18 1.0 19 0.0 20 4.0 21 6.0 22 2.0 23 5.0 24 10.0 25 4.0 26 6.0 27 10.0 28 15.0 29 20.0 30 26.0 31 35.0 32 82.0 33 104.0 34 245.0 35 622.0 36 2571.0 37 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.375 24.025 7.875 22.725 2 30.2 24.95 27.525 17.325 3 22.2 26.025 32.85 18.925 4 25.074999999999996 30.45 24.0 20.474999999999998 5 27.750000000000004 32.85 20.599999999999998 18.8 6 24.175 37.225 18.35 20.25 7 22.900000000000002 21.475 33.300000000000004 22.325 8 21.099999999999998 25.275 26.55 27.075 9 21.9 24.25 29.225 24.625 10-14 25.39 26.8 25.290000000000003 22.52 15-19 25.224999999999998 26.865 25.790000000000003 22.12 20-24 24.72 26.515 26.490000000000002 22.275 25-29 25.295 27.01 25.974999999999998 21.72 30-34 25.03 27.029999999999998 25.64 22.3 35-39 23.525 27.74 27.139999999999997 21.595 40-44 23.93 26.25 28.865000000000002 20.955 45-49 25.28 26.66 26.965 21.095 50-54 24.0 26.950000000000003 27.36 21.69 55-59 23.849999999999998 27.805000000000003 26.355 21.990000000000002 60-64 23.77 25.790000000000003 28.804999999999996 21.634999999999998 65-69 24.70747074707471 26.3976397639764 28.16781678167817 20.72707270727073 70-74 24.303087933536858 24.87863470296782 28.767328962514387 22.050948400980932 75-79 24.455569461827285 25.351689612015022 28.715894868585735 21.476846057571965 80-84 24.108709822995536 25.61299704156847 29.579301007872438 20.698992127563557 85-89 24.01987852015461 25.78685808945334 28.24155413884845 21.951709251543598 90-94 23.732904263877717 27.081657280772326 27.695092518101365 21.49034593724859 95-99 23.770656993147924 24.853889560661024 28.622531237404274 22.75292220878678 100-104 24.01578787572108 25.189758121647603 28.10950308673211 22.6849509158992 105-109 24.541694326711944 26.875351069805443 28.024306796711436 20.55864780677118 110-114 24.035485867546935 26.098617701671134 28.46606148132866 21.39983494945327 115-119 22.631661360196254 26.770708283313326 27.85635993527846 22.74127042121196 120-124 23.79595927625679 26.60231049216648 28.20066466213008 21.401065569446644 125-129 24.608393477679765 26.228281208233096 28.201015771184174 20.962309542902968 130-134 25.318100601007092 26.13568682657426 28.17694515133467 20.36926742108398 135-139 23.12962758315836 26.18521383578296 28.378826389656314 22.306332191402365 140-144 23.03147369617089 26.286785592546302 28.451312350869216 22.23042836041359 145-149 23.008337193144975 27.599583140342755 28.132237146827233 21.25984251968504 150 26.662760035159682 26.018165836507475 27.219455024904775 20.09961910342807 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 0.5 25 0.0 26 0.0 27 0.5 28 1.0 29 4.0 30 11.5 31 17.5 32 30.5 33 32.5 34 35.0 35 48.0 36 77.5 37 111.0 38 148.0 39 191.0 40 180.0 41 202.0 42 229.0 43 223.0 44 277.0 45 284.5 46 217.5 47 196.0 48 160.0 49 120.0 50 115.0 51 101.0 52 96.0 53 77.0 54 69.5 55 72.5 56 78.0 57 85.5 58 90.0 59 77.0 60 46.0 61 46.5 62 53.0 63 48.0 64 31.0 65 16.5 66 15.0 67 12.5 68 16.5 69 15.5 70 10.5 71 7.5 72 5.0 73 7.0 74 5.0 75 1.5 76 2.5 77 3.5 78 2.0 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 0.0 70-71 3.0 72-73 1.0 74-75 0.0 76-77 0.0 78-79 3.0 80-81 3.0 82-83 3.0 84-85 1.0 86-87 2.0 88-89 1.0 90-91 3.0 92-93 6.0 94-95 2.0 96-97 2.0 98-99 11.0 100-101 5.0 102-103 7.0 104-105 21.0 106-107 15.0 108-109 17.0 110-111 12.0 112-113 21.0 114-115 20.0 116-117 18.0 118-119 13.0 120-121 16.0 122-123 23.0 124-125 18.0 126-127 21.0 128-129 15.0 130-131 22.0 132-133 26.0 134-135 23.0 136-137 38.0 138-139 56.0 140-141 35.0 142-143 20.0 144-145 31.0 146-147 26.0 148-149 26.0 150-151 3413.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.400000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 52.42456896551724 24.325 2 20.474137931034484 19.0 3 11.691810344827585 16.275000000000002 4 6.627155172413793 12.3 5 3.2866379310344827 7.625 6 1.8318965517241377 5.1 7 1.4547413793103448 4.725 8 0.7004310344827587 2.6 9 0.3771551724137931 1.575 >10 1.1314655172413792 6.4750000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTT 19 0.475 No Hit GTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGA 17 0.42500000000000004 No Hit GTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTTG 14 0.35000000000000003 No Hit TGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTT 14 0.35000000000000003 No Hit GGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAAC 14 0.35000000000000003 No Hit AGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTT 14 0.35000000000000003 No Hit GTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGC 13 0.325 No Hit GTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTT 13 0.325 No Hit GCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAG 13 0.325 No Hit GAAGGAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGA 12 0.3 No Hit GTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGA 12 0.3 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 11 0.27499999999999997 No Hit GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 11 0.27499999999999997 No Hit GCTCAAGAAGGTGCCTCCAACACGTGCGAAATCACCAAAGTTTACAAGGA 11 0.27499999999999997 No Hit ACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTG 11 0.27499999999999997 No Hit GGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTC 10 0.25 No Hit GGATGATGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTT 10 0.25 No Hit GCTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAA 10 0.25 No Hit GGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGA 10 0.25 No Hit CGGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGT 10 0.25 No Hit GGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAG 10 0.25 No Hit GCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACA 9 0.22499999999999998 No Hit GGCCTCTTCCAATCCTTTTGCCGGCTCGTACATGCCCAGCAGCGGCCACC 9 0.22499999999999998 No Hit GGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTT 9 0.22499999999999998 No Hit GAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTT 9 0.22499999999999998 No Hit GTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTACAT 9 0.22499999999999998 No Hit ATTGATTAATACGGCGGCCGCGTCGTCGATCTCGCTGCAAGCCTGCAAGC 9 0.22499999999999998 No Hit CGGTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAG 9 0.22499999999999998 No Hit GCAATGTGTTCAAGAAAATGCTTGTGAAGAAATATGAACGTGGTTGTGTC 8 0.2 No Hit AAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTA 8 0.2 No Hit ATTGACTATTACTTGGAGTATGAACCTCATCCATCATCAACTAAAAACTT 8 0.2 No Hit GGGAGAGAGCCTCGGGGGAAGGATCAAGAGAGGCTCGCCCGCGCGCGTGA 8 0.2 No Hit GTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTGCTCAGATA 8 0.2 No Hit GGATGAGCTCGGGTTTGACATCGTGCAGGGGCAAGCAATGTGTTCAAGAA 8 0.2 No Hit GATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGTG 8 0.2 No Hit GTTAGATCAAATAAATGAGGCATGCAAATTCTTGGATGAAAGTTGGTCCC 8 0.2 No Hit GCTCCATGCCCGCAACACGTACACTGGAACGGGGATGATGTAGAGATGCT 8 0.2 No Hit AGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGA 8 0.2 No Hit GAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCA 8 0.2 No Hit GGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAAT 8 0.2 No Hit GATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGT 8 0.2 No Hit AGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGG 7 0.17500000000000002 No Hit CTTTAAAACAACTAAGGAAGTCCTTGGTCATTAGAGCTAAACCCATGCCA 7 0.17500000000000002 No Hit AGAAGGATGACCAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAG 7 0.17500000000000002 No Hit GGGAGATGTTTGCTGACTCCTGCCGTAGGCTCAGGATCATGAAAGGTTCA 7 0.17500000000000002 No Hit AGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGAC 7 0.17500000000000002 No Hit GAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTT 7 0.17500000000000002 No Hit CATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGC 7 0.17500000000000002 No Hit CAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATT 7 0.17500000000000002 No Hit GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 7 0.17500000000000002 No Hit AACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGA 7 0.17500000000000002 No Hit GCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGC 7 0.17500000000000002 No Hit CGGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTA 7 0.17500000000000002 No Hit CAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATTGACGCTG 7 0.17500000000000002 No Hit CATGCCCTTTGGCCTGTTGCCATCGGTGTGTATATCACTAGTTTTTGTTT 7 0.17500000000000002 No Hit GAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCAT 7 0.17500000000000002 No Hit AGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTA 7 0.17500000000000002 No Hit GGTGGCCACAACTGCTGCAGTCAGGACAATCATTCCTAAACTTGGCACGG 7 0.17500000000000002 No Hit GATTAATACGGCGGCCGCGTCGTCGATCTCGCTGCAAGCCTGCAAGCTAT 7 0.17500000000000002 No Hit GCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAA 7 0.17500000000000002 No Hit GGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTT 7 0.17500000000000002 No Hit AGGGAACCCCTCTGCGAAGCATCGAGATTGGCACTTCGCTATCCCATGAA 7 0.17500000000000002 No Hit GTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCG 7 0.17500000000000002 No Hit CTTGAAATTGTGCAGGATGAGCTCGGGTTTGACATCGTGCAGGGGCAAGC 7 0.17500000000000002 No Hit AGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAAGGAGACTACCAAGAAT 7 0.17500000000000002 No Hit AAGCAGTGATACCGCAGTTATTATGTATACAAGTGGAAGTACAGGTCTGC 7 0.17500000000000002 No Hit CCCAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTG 7 0.17500000000000002 No Hit GTTCAATTTTGCTAACAGAGTGAAGGAGAGATTTCAAGGCCTTTTTGATG 7 0.17500000000000002 No Hit GTGGAGTACGAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGC 6 0.15 No Hit CAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTGAC 6 0.15 No Hit TGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTTTATGGG 6 0.15 No Hit ATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCC 6 0.15 No Hit CCGCAACACGTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTT 6 0.15 No Hit AAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTAGCCAGCC 6 0.15 No Hit ATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTG 6 0.15 No Hit CTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAG 6 0.15 No Hit ATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGACCTTAAAAA 6 0.15 No Hit GCTGAAGGCTTTCTGAAAGATCATGGAGCAGAAAAAGCGCCGCGAATGGC 6 0.15 No Hit GAGAAGACGAAGAGAGAAAGAAAAAAAATCGAACAATCCGGGAGGAAAAG 6 0.15 No Hit GCTCAGGATCATGAAAGGTTCAGATGCAATTGGACTCGCTCCAAGGGCAG 6 0.15 No Hit GCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGT 6 0.15 No Hit GGATGATTGGACCCCTGTTTTGGAGCAAGGCAGGCCAGTCTTGTCTCAGT 6 0.15 No Hit GCCAACAAAGCGCAATGCTCGCCAAAGAATGGTGTAGCTGCAAAGACCAG 6 0.15 No Hit GAGACGCCGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAAAAAG 6 0.15 No Hit TTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCAA 6 0.15 No Hit GCAAGCTATAGAGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGA 6 0.15 No Hit AGGATGAGCTCGGGTTTGACATCGTGCAGGGGCAAGCAATGTGTTCAAGA 6 0.15 No Hit GATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAAACCCTTTGA 6 0.15 No Hit GCTTTTTGAGACAGAGAAGGAGACTACCAAGAATCTGCCTGACTTCAAGA 6 0.15 No Hit GGGGAAAATTGTTGCTTCAATGCATCACGGATTGACTATTACTTGGAGTA 6 0.15 No Hit GGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCAT 6 0.15 No Hit CCTACTCTTATGATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGC 6 0.15 No Hit GGCCGACACGCCGTGGCATTATAGCGAGGATGGGGTGGATCTTGTTGTTA 6 0.15 No Hit GGACATCCATTTAAAGCATTACAGAAACTTACAGACGAGAGGAATTTAGT 6 0.15 No Hit AAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTGACAAGCCACACG 6 0.15 No Hit CGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCCTTTCCAGATGC 6 0.15 No Hit ATTAGATTCTCTGTGTGATGATGGACATCTGGACTGCAATAATATGCTGT 6 0.15 No Hit ATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATTGACGCTGAGTTG 6 0.15 No Hit GCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGA 6 0.15 No Hit CATCGTCACAGCATTGGGAACCCTAAAGATGCCAACAAAGCGCAATGCTC 6 0.15 No Hit AGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCT 6 0.15 No Hit GTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTAT 6 0.15 No Hit GGCTGCAACAAAATCTGCAAAGGCGAAACTGCGAGGTCAGATTTCACCTA 5 0.125 No Hit GAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGAAGTCTTT 5 0.125 No Hit CAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCT 5 0.125 No Hit CTGTCTTGAGACGATCTTTGCCTAGCTCATGCTCGTCAGTAGTACCTGAA 5 0.125 No Hit GATTAGTTCTGCTGCACTTCTTTGTCCAATTTCTTACCTTGATCATGTTA 5 0.125 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 5 0.125 No Hit AGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGAAGTCTTTT 5 0.125 No Hit ATTACATCTGTGCAAGCTATTTATGTGCCTGCTGATGACTTGACAGATCC 5 0.125 No Hit AGATTCTCTGTGTGATGATGGACATCTGGACTGCAATAATATGCTGTCTT 5 0.125 No Hit ACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTGCCCTT 5 0.125 No Hit CGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGAGGGGT 5 0.125 No Hit CTCTGTGTGCTGCCCTTCTTGCGCCCGGCTGCGGGTATCCGCTTCGTGAT 5 0.125 No Hit GATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAA 5 0.125 No Hit GGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAAGGAGACTACCA 5 0.125 No Hit GCGGCCACCCTTATGGTCAGCTCCATGCCCGCAACACGTACACTGGAACG 5 0.125 No Hit GCAACACGTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGC 5 0.125 No Hit GGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAG 5 0.125 No Hit GCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGC 5 0.125 No Hit CTTTTGCCGGCTCGTACATGCCCAGCAGCGGCCACCCTTATGGTCAGCTC 5 0.125 No Hit GGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGG 5 0.125 No Hit GAGAGGCTCGCCCGCGCGCGTGATGAATTGATTAATACGGCGGCCGCGTC 5 0.125 No Hit GAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTT 5 0.125 No Hit CAAGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAGAGA 5 0.125 No Hit GATGAGCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAAT 5 0.125 No Hit GTCTAATGGAAGTATGGAACTTGAAATTGTGCAGGATGAGCTCGGGTTTG 5 0.125 No Hit ATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGA 5 0.125 No Hit GGAAAGTATGTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATT 5 0.125 No Hit CATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTC 5 0.125 No Hit CGATAACAGGGGAAAATTGTTGCTTCAATGCATCACGGATTGACTATTAC 5 0.125 No Hit GATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGC 5 0.125 No Hit TTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGAT 5 0.125 No Hit GAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCT 5 0.125 No Hit TGATAAGATAAAATCAAAACCTTGAAACTGCACCTAGTAACATATCTCTG 5 0.125 No Hit ATTCAGCGGTTCCTTAGCCAGCCTTTTCATGTTGCTGAAGTGTTCACAGG 5 0.125 No Hit TGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAG 5 0.125 No Hit CATACAGATCCAAGATTACCATCAAGCAGTGATACCGCAGTTATTATGTA 5 0.125 No Hit CTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCGGGGGAAGGATCA 5 0.125 No Hit AGCTCAGGTCGACCATCACGCCCGGTACGGTGCTGATCCTGCTCGCCGGT 5 0.125 No Hit GGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCGG 5 0.125 No Hit CCCTCCAAAGGCTGAGCTCAAGAAGGTGCCTCCAACACGTGCGAAATCAC 5 0.125 No Hit GTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAATTTTGGA 5 0.125 No Hit GATCGGGCGTGCGAGCGCGGGACCTTGTCCTTATCTTGTACGTGCCCCGG 5 0.125 No Hit AGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAAC 5 0.125 No Hit GTTCGCCCTGGATGCTGACTTCATTCTGACTTACACTGATGAGGACGGGG 5 0.125 No Hit GTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGA 5 0.125 No Hit CGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCG 5 0.125 No Hit GGGAGCTTTTTGAGACAGAGAAGGAGACTACCAAGAATCTGCCTGACTTC 5 0.125 No Hit CAGCTGAACTTTCGGAGTGATTTGGGGGTTCAAATATTAGATTCTCTGTG 5 0.125 No Hit CTATACCGAACTCCTCACATTAATTGGCTGTTAAAAATCTTAACTCCAAA 5 0.125 No Hit GCGTAGAAATCTCCTCACATTTTAACTTTTTGTGGTCAACCTGTTGTGCC 5 0.125 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 5 0.125 No Hit GACAAAAGGAGACGTTTCTGTACTGAAACCCACTCTTATGATTTCAGTTC 5 0.125 No Hit TGATAAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTAGCC 5 0.125 No Hit GGAGCTCGCCGCCGTTCGACGGCCTCGCGCCGGGACCCAACTCCGAATGG 5 0.125 No Hit CTTTGCTAGGGACAAGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTTT 5 0.125 No Hit GTCTTCGATAACAGGGGAAAATTGTTGCTTCAATGCATCACGGATTGACT 5 0.125 No Hit ATCTCCTCACATTTTAACTTTTTGTGGTCAACCTGTTGTGCCTTTTATTT 5 0.125 No Hit GCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCG 5 0.125 No Hit TGATTTGCTGGGCAGAAATAAAAAAACATGTGTTAATACGATCTCACATG 5 0.125 No Hit CAGCCACCACCAGCGCAGCATCTCATCGGTCACATCGTCACAGCATTGGG 5 0.125 No Hit GTCAGGACAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.025 52-53 0.0 0.0 0.0 0.0 0.025 54-55 0.0 0.0 0.0 0.0 0.025 56-57 0.0 0.0 0.0 0.0 0.025 58-59 0.0 0.0 0.0 0.0 0.025 60-61 0.0 0.0 0.0 0.0 0.025 62-63 0.0 0.0 0.0 0.0 0.025 64-65 0.0 0.0 0.0 0.0 0.025 66-67 0.0 0.0 0.0 0.0 0.025 68-69 0.0 0.0 0.0 0.0 0.025 70-71 0.0 0.0 0.0 0.0 0.025 72-73 0.0 0.0 0.0 0.0 0.025 74-75 0.0 0.0 0.0 0.0 0.025 76-77 0.0 0.0 0.0 0.0 0.025 78-79 0.0 0.0 0.0 0.0 0.025 80-81 0.0 0.0 0.0 0.0 0.025 82-83 0.0 0.0 0.0 0.0 0.025 84-85 0.0 0.0 0.0 0.0 0.025 86-87 0.0 0.0 0.0 0.0 0.025 88-89 0.0 0.0 0.0 0.0 0.025 90-91 0.0 0.0 0.0 0.0 0.025 92-93 0.0 0.0 0.0 0.0 0.025 94-95 0.0 0.0 0.0 0.0 0.025 96-97 0.0 0.0 0.0 0.0 0.025 98-99 0.0 0.0 0.0 0.0 0.025 100-101 0.0 0.0 0.0 0.0 0.025 102-103 0.0 0.0 0.0 0.0 0.025 104-105 0.0 0.0 0.0 0.0 0.025 106-107 0.0 0.0 0.0 0.0 0.025 108-109 0.0 0.0 0.0 0.0 0.025 110-111 0.0 0.0 0.0 0.0 0.025 112-113 0.0 0.0 0.0 0.0 0.025 114-115 0.0 0.0 0.0 0.0 0.025 116-117 0.0 0.0 0.0 0.0 0.025 118-119 0.0 0.0 0.0 0.0 0.025 120-121 0.0 0.0 0.0 0.0 0.025 122-123 0.0 0.0 0.0 0.0 0.025 124-125 0.0 0.0 0.0 0.0 0.025 126-127 0.0 0.0 0.0 0.0 0.025 128-129 0.0 0.0 0.0 0.0 0.025 130-131 0.0 0.0 0.0 0.0 0.025 132-133 0.0 0.0 0.0 0.0 0.025 134-135 0.0 0.0 0.0 0.0 0.025 136-137 0.0 0.0 0.0 0.0 0.05 138 0.0 0.0 0.0 0.0 0.05 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1685620 spots for ERR5262781.sra Written 1685620 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra Read 1685601 spots for ERR5262781.sra Written 1685601 spots for ERR5262781.sra SRR ids: ['ERR5262781.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_am__6qvn ERR5262781.sra spots: 33712039 blocks: [[1, 1685601], [1685602, 3371202], [3371203, 5056803], [5056804, 6742404], [6742405, 8428005], [8428006, 10113606], [10113607, 11799207], [11799208, 13484808], [13484809, 15170409], [15170410, 16856010], [16856011, 18541611], [18541612, 20227212], [20227213, 21912813], [21912814, 23598414], [23598415, 25284015], [25284016, 26969616], [26969617, 28655217], [28655218, 30340818], [30340819, 32026419], [32026420, 33712039]] ERR5262781 file size 11037277 ERR5262781 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262781 ERR5262781_1.fastq ERR5262781_2.fastq Input file: ERR5262781_1.fastq Paired file: ERR5262781_2.fastq trimmed: ERR5262781-trimmed-pair1.fastq, ERR5262781-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:17:42 2024 >> started Fri Dec 6 11:18:58 2024 >> done (76.320s) 33712039 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 1 ( 0.00%) empty read pairs filtered out after trimming by size control 33712038 (100.00%) read pairs available; of these: 10219 ( 0.03%) trimmed read pairs available after processing 33701819 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 22 1 0.00% 23 0 0.00% 24 0 0.00% 25 2 0.00% 26 0 0.00% 27 1 0.00% 28 4 0.00% 29 0 0.00% 30 0 0.00% 31 4 0.00% 32 1 0.00% 33 2 0.00% 34 1 0.00% 35 1 0.00% 36 4 0.00% 37 3 0.00% 38 6 0.00% 39 6 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 1 0.00% 44 1 0.00% 45 3 0.00% 46 0 0.00% 47 2 0.00% 48 3 0.00% 49 321 0.00% 50 367 0.00% 51 445 0.00% 52 439 0.00% 53 484 0.00% 54 571 0.00% 55 620 0.00% 56 707 0.00% 57 716 0.00% 58 869 0.00% 59 1027 0.00% 60 1097 0.00% 61 1378 0.00% 62 1511 0.00% 63 1725 0.01% 64 1810 0.01% 65 1930 0.01% 66 2162 0.01% 67 2376 0.01% 68 2764 0.01% 69 3294 0.01% 70 3650 0.01% 71 4254 0.01% 72 5053 0.01% 73 5559 0.02% 74 5974 0.02% 75 6601 0.02% 76 7218 0.02% 77 7876 0.02% 78 8774 0.03% 79 9828 0.03% 80 11318 0.03% 81 12654 0.04% 82 14293 0.04% 83 15986 0.05% 84 17332 0.05% 85 19519 0.06% 86 20353 0.06% 87 21748 0.06% 88 22965 0.07% 89 24796 0.07% 90 27023 0.08% 91 29467 0.09% 92 32098 0.10% 93 34654 0.10% 94 37645 0.11% 95 40041 0.12% 96 41785 0.12% 97 43549 0.13% 98 44919 0.13% 99 46735 0.14% 100 48904 0.15% 101 51522 0.15% 102 55150 0.16% 103 59041 0.18% 104 61510 0.18% 105 65148 0.19% 106 66867 0.20% 107 67847 0.20% 108 68868 0.20% 109 71243 0.21% 110 73098 0.22% 111 76542 0.23% 112 79266 0.24% 113 81677 0.24% 114 87037 0.26% 115 89263 0.26% 116 91629 0.27% 117 93067 0.28% 118 94410 0.28% 119 95568 0.28% 120 96122 0.29% 121 98843 0.29% 122 100963 0.30% 123 104043 0.31% 124 109880 0.33% 125 111316 0.33% 126 113221 0.34% 127 115356 0.34% 128 116017 0.34% 129 117524 0.35% 130 117763 0.35% 131 119109 0.35% 132 121972 0.36% 133 124667 0.37% 134 128186 0.38% 135 131138 0.39% 136 132178 0.39% 137 133060 0.39% 138 133548 0.40% 139 136727 0.41% 140 137422 0.41% 141 139316 0.41% 142 142253 0.42% 143 144309 0.43% 144 147306 0.44% 145 149029 0.44% 146 150522 0.45% 147 285938 0.85% 148 141638 0.42% 149 142344 0.42% 150 27642345 82.00% 33712038 reads passed initial QC criterion=sequence-density sequence-density=0.15 sequence-density-rank=1 fanout-score=5.12 fanout-score-rank=34 prefix-density=0.25 prefix-fanout=3.1 sequence=GATGCCCACGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=45 fanout-score=110.21 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=6.0 sequence=CTTGGCACCACCCTTCAAGTGAGCTGCGGCCTTGTCCTTGTCAGTGAAGACACCAGTGGACTCCACGACATAATCGGCACCAGCCTCAGCCCATGGGATCTCCTCAGGGTTCCTGACGCCGAAGACGGTGACGGACTTCTCGCCGAAGAGGAGGGTCTTGTCGTTCTTGAGCTTGATGTCGCTGTGCTTCCAGTGGCCGTGGACGGTGTCGTACTTGAACATGTAGGTCATGTACTCGGTGGTGATGAAGGGGTCGTTG criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=6.23 fanout-score-rank=26 prefix-density=0.67 prefix-fanout=3.7 sequence=TCTTCCTCCTGGCCGCCGGCGTCCTCTTCGCCGCGGCCTCCACCTCCAGCTCCAGGGA criterion=fanout-score sequence-density=0.12 sequence-density-rank=9 fanout-score=97.24 fanout-score-rank=1 prefix-density=0.70 prefix-fanout=16.8 sequence=CGCCGCCGCCGC ERR5262781 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:19:34 Started mapping on | Dec 06 11:19:34 Finished on | Dec 06 11:22:03 Mapping speed, Million of reads per hour | 814.52 Number of input reads | 33712038 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 32582339 Uniquely mapped reads % | 96.65% Average mapped length | 289.95 Number of splices: Total | 31111923 Number of splices: Annotated (sjdb) | 28911776 Number of splices: GT/AG | 30652545 Number of splices: GC/AG | 381391 Number of splices: AT/AC | 19210 Number of splices: Non-canonical | 58777 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.17 Insertion rate per base | 0.01% Insertion average length | 2.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 382241 % of reads mapped to multiple loci | 1.13% Number of reads mapped to too many loci | 870 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.21% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 747458 747458 747458 N_multimapping 382241 382241 382241 N_noFeature 1412705 31695007 1717066 N_ambiguous 685408 4222 103150 UnstrandedReadsAssigned:30484226 PositiveStrandReadsAssigned:883110 NegativeStrandReadsAssigned:30762123 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262781 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262781-trimmed-pair1.fastq ERR5262781-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 33,712,038 reads, 31,153,777 reads pseudoaligned [quant] estimated average fragment length: 253.548 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,296 rounds 52973 ERR5262781.ke.tsv 35125 ERR5262781.se.tsv 88098 total ==> ERR5262781.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 683.859 0 0 PNS24247 1044 791.452 163.738 9.46106 PNS24249 1928 1675.45 429.321 11.7183 PNS24246 1044 791.452 163.738 9.46106 PNS24248 1044 791.452 163.738 9.46106 PNS24244 1471 1218.45 234.465 8.80003 PNS24243 293 105.413 0 0 KQK14069 1603 1350.45 63868 2162.81 KQK14071 474 246.876 1174.97 217.652 ==> ERR5262781.se.tsv <== BRADI_1g14170v3 68438 BRADI_1g53295v3 306 BRADI_1g59795v3 1133 BRADI_1g07683v3 0 BRADI_1g00485v3 32 BRADI_1g20270v3 1200 BRADI_1g74790v3 3022 BRADI_1g09890v3 0 BRADI_1g77505v3 528 BRADI_1g48960v3 0 ERR5262781 completed mapping pipeline successfully