Starting /dee2/code/volunteer_pipeline.sh ERR5262782 current disk space = 1551292276736 free memory = 1594915264 ERR5262782 SRAfilesize dfb1082ff31db3ad388dcc876f682cab ERR5262782.sra ERR5262782.sra file validated ERR5262782 is paired end ERR5262782 is conventional basespace ERR5262782 read1 length is 81-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262782_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 81-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6615 37.0 37.0 37.0 37.0 37.0 2 36.4375 37.0 37.0 37.0 37.0 37.0 3 36.6025 37.0 37.0 37.0 37.0 37.0 4 36.6335 37.0 37.0 37.0 37.0 37.0 5 36.6785 37.0 37.0 37.0 37.0 37.0 6 36.7055 37.0 37.0 37.0 37.0 37.0 7 36.6465 37.0 37.0 37.0 37.0 37.0 8 36.6595 37.0 37.0 37.0 37.0 37.0 9 36.628 37.0 37.0 37.0 37.0 37.0 10-14 36.62 37.0 37.0 37.0 37.0 37.0 15-19 36.511900000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.49309999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.5208 37.0 37.0 37.0 37.0 37.0 30-34 36.4731 37.0 37.0 37.0 37.0 37.0 35-39 36.392900000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.3892 37.0 37.0 37.0 37.0 37.0 45-49 36.3633 37.0 37.0 37.0 37.0 37.0 50-54 36.3634 37.0 37.0 37.0 37.0 37.0 55-59 36.320100000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.1893 37.0 37.0 37.0 37.0 37.0 65-69 36.1279 37.0 37.0 37.0 37.0 37.0 70-74 36.0824 37.0 37.0 37.0 37.0 37.0 75-79 36.030899999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.99535101275319 37.0 37.0 37.0 37.0 37.0 85-89 35.90633988395223 37.0 37.0 37.0 37.0 37.0 90-94 35.92165206508135 37.0 37.0 37.0 37.0 37.0 95-99 35.855220596986136 37.0 37.0 37.0 37.0 37.0 100-104 35.81516213050516 37.0 37.0 37.0 37.0 37.0 105-109 35.870775092130074 37.0 37.0 37.0 37.0 37.0 110-114 35.763395163678936 37.0 37.0 37.0 37.0 37.0 115-119 35.80001962880779 37.0 37.0 37.0 37.0 37.0 120-124 35.82272045133715 37.0 37.0 37.0 37.0 37.0 125-129 35.835554250760325 37.0 37.0 37.0 37.0 37.0 130-134 35.75320257606469 37.0 37.0 37.0 37.0 37.0 135-139 35.630851724407464 37.0 37.0 37.0 37.0 37.0 140-144 35.66652421732009 37.0 37.0 37.0 37.0 37.0 145-149 35.40684537095683 37.0 37.0 37.0 37.0 37.0 150 35.508250825082506 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 2.0 23 2.0 24 2.0 25 2.0 26 6.0 27 7.0 28 10.0 29 22.0 30 24.0 31 34.0 32 51.0 33 102.0 34 202.0 35 398.0 36 2827.0 37 305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.675 10.8 7.825 36.7 2 18.884442221110557 11.480740370185092 30.71535767883942 38.91945972986493 3 13.425 18.925 31.025000000000002 36.625 4 20.95 20.825 27.425 30.8 5 21.175 31.3 21.925 25.6 6 19.35 35.75 24.4 20.5 7 14.725 29.299999999999997 39.375 16.6 8 12.425 26.25 29.349999999999998 31.974999999999998 9 16.875 22.825 36.75 23.549999999999997 10-14 19.73 27.395000000000003 27.060000000000002 25.814999999999998 15-19 17.945 26.985 26.345000000000002 28.725 20-24 17.29 24.905 29.775000000000002 28.03 25-29 19.55 28.535 28.535 23.380000000000003 30-34 20.025000000000002 25.8 27.82 26.355 35-39 19.08 26.495 27.77 26.655 40-44 18.32 30.43 26.889999999999997 24.36 45-49 16.825000000000003 30.570000000000004 28.645 23.96 50-54 18.61 29.24 28.634999999999998 23.515 55-59 18.77 31.185000000000002 26.590000000000003 23.455000000000002 60-64 17.68 31.735000000000003 26.915 23.669999999999998 65-69 18.64 31.28 29.185 20.895 70-74 20.674999999999997 31.105 27.900000000000002 20.32 75-79 21.634999999999998 33.095 24.740000000000002 20.53 80-84 21.39820973145972 31.464719707956196 26.904035605340802 20.233034955243287 85-89 22.28614307153577 32.341170585292645 25.65782891445723 19.714857428714357 90-94 24.055068836045056 32.12015018773467 24.891113892365457 18.933667083854818 95-99 21.503330161750714 31.709149181230906 27.662877460063097 19.12464319695528 100-104 20.787164702933065 32.40912509400852 27.676109300576584 19.127600902481827 105-109 23.862207694241892 30.093034950968068 24.707065627357306 21.33769172743274 110-114 22.30501988822315 29.797089773928803 25.633150395246968 22.26473994260108 115-119 21.39248181083266 30.370856911883585 27.152384801940176 21.08427647534357 120-124 21.47391944740718 32.10422063080908 23.982934633551732 22.43892528823201 125-129 19.185242121445043 31.53471688444786 25.58544709198053 23.69459390212657 130-134 21.9751166407465 33.21410057024365 24.59305339554173 20.21772939346812 135-139 24.405605416469847 28.693644045557132 26.6887104393009 20.212040098672123 140-144 25.01068832834545 28.64471996579735 24.593843522873023 21.750748182984182 145-149 21.127909506199696 27.670219708505545 27.8333695888623 23.368501196432454 150 25.687568756875688 24.669966996699667 27.282728272827285 22.35973597359736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.5 14 0.5 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 0.0 22 3.0 23 4.5 24 7.5 25 9.0 26 3.5 27 1.5 28 6.0 29 9.5 30 13.0 31 27.0 32 43.0 33 50.5 34 78.0 35 114.0 36 131.0 37 182.5 38 219.5 39 196.0 40 245.0 41 278.5 42 295.0 43 294.0 44 286.0 45 285.5 46 180.0 47 131.0 48 108.5 49 52.0 50 28.5 51 21.5 52 26.0 53 30.0 54 59.0 55 96.0 56 88.5 57 65.5 58 100.0 59 100.5 60 57.5 61 32.5 62 4.5 63 4.5 64 4.0 65 4.5 66 9.0 67 5.5 68 1.5 69 1.5 70 0.0 71 0.5 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 80-81 1.0 82-83 0.0 84-85 0.0 86-87 2.0 88-89 2.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 0.0 98-99 3.0 100-101 1.0 102-103 5.0 104-105 10.0 106-107 0.0 108-109 1.0 110-111 1.0 112-113 4.0 114-115 9.0 116-117 3.0 118-119 10.0 120-121 9.0 122-123 12.0 124-125 16.0 126-127 16.0 128-129 18.0 130-131 18.0 132-133 23.0 134-135 14.0 136-137 16.0 138-139 39.0 140-141 21.0 142-143 27.0 144-145 38.0 146-147 8.0 148-149 36.0 150-151 3636.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.15 #Duplication Level Percentage of deduplicated Percentage of total 1 46.181172291296626 13.0 2 17.939609236234457 10.100000000000001 3 10.74600355239787 9.075 4 6.394316163410302 7.199999999999999 5 4.7957371225577266 6.75 6 3.463587921847247 5.8500000000000005 7 1.5985790408525755 3.15 8 1.4209591474245116 3.2 9 0.6216696269982238 1.575 >10 6.483126110124333 32.65 >50 0.3552397868561279 7.449999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 100 2.5 No Hit GCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGG 84 2.1 No Hit GTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 57 1.425 No Hit CCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 57 1.425 No Hit GGCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCC 50 1.25 No Hit CTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATG 40 1.0 No Hit CGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTC 39 0.975 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 39 0.975 No Hit CCAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGC 37 0.9249999999999999 No Hit GCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTACTGGGTCTCAA 36 0.8999999999999999 No Hit GTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAACATGCACG 31 0.775 No Hit GTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAA 31 0.775 No Hit GGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAAT 28 0.7000000000000001 No Hit GGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGG 27 0.675 No Hit GCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATG 26 0.65 No Hit GCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGT 25 0.625 No Hit GCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCT 25 0.625 No Hit CCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACAC 25 0.625 No Hit TAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAG 24 0.6 No Hit AGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 23 0.575 No Hit ACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATA 22 0.5499999999999999 No Hit CCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACA 21 0.525 No Hit AGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTT 20 0.5 No Hit GGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAA 20 0.5 No Hit GTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCA 19 0.475 No Hit CACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTG 18 0.44999999999999996 No Hit CCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGA 18 0.44999999999999996 No Hit CTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTT 18 0.44999999999999996 No Hit GTCAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAAT 17 0.42500000000000004 No Hit CCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCC 17 0.42500000000000004 No Hit CCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACAT 17 0.42500000000000004 No Hit GCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAAC 17 0.42500000000000004 No Hit CCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAAC 16 0.4 No Hit CAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCAC 16 0.4 No Hit GCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACAC 16 0.4 No Hit GTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGC 16 0.4 No Hit GCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATA 16 0.4 No Hit GCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATAC 16 0.4 No Hit CACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCT 15 0.375 No Hit GTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCA 15 0.375 No Hit GTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCG 15 0.375 No Hit CCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 15 0.375 No Hit AAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATT 15 0.375 No Hit CCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 15 0.375 No Hit CTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGA 14 0.35000000000000003 No Hit CTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCG 14 0.35000000000000003 No Hit CTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCA 14 0.35000000000000003 No Hit GTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCT 14 0.35000000000000003 No Hit CCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACAT 14 0.35000000000000003 No Hit CTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 14 0.35000000000000003 No Hit CACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCAT 14 0.35000000000000003 No Hit GCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGA 14 0.35000000000000003 No Hit GGAATGTTATTGCCATCAGTAGATTCGCCAAAAAAGTGCTCCCGGTTCAG 13 0.325 No Hit CTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAA 13 0.325 No Hit CTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGAT 12 0.3 No Hit CTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAG 12 0.3 No Hit CTTCAGAATCCAGTGTTGTCCTTGGCAGTTGCCTACGGAGCAAAACGATT 12 0.3 No Hit CTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCCT 12 0.3 No Hit GGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGT 12 0.3 No Hit GCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATAT 12 0.3 No Hit GTCCCCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCTGACAAATC 12 0.3 No Hit GTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAG 11 0.27499999999999997 No Hit GCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCA 11 0.27499999999999997 No Hit CCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTT 11 0.27499999999999997 No Hit GTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCA 11 0.27499999999999997 No Hit GACCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCACAGAG 11 0.27499999999999997 No Hit GCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGT 11 0.27499999999999997 No Hit GGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGA 11 0.27499999999999997 No Hit CTCCAATCAGGTACAATTTTTTTCTCCCACTCAAAGCTCGACCTCAAACC 11 0.27499999999999997 No Hit CAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGAT 10 0.25 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 10 0.25 No Hit GGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCA 10 0.25 No Hit CCTCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 10 0.25 No Hit GCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAA 10 0.25 No Hit GCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCC 10 0.25 No Hit TGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCT 10 0.25 No Hit GTTCAGTTCACATTGCTACCCTAGTTAGAAAATTCCACCAAAATACGCAA 10 0.25 No Hit GGCAGTTTTACTAAAGTGAGAAGTGGCACTACTGACTGCATAAACTGTGC 9 0.22499999999999998 No Hit CGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAAATA 9 0.22499999999999998 No Hit TGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCAT 9 0.22499999999999998 No Hit GGCTGCATCAGTAGCTGCGCCTACAGAAAATTATCGATTTGTTTGAGATC 9 0.22499999999999998 No Hit GTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCA 9 0.22499999999999998 No Hit CGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATC 9 0.22499999999999998 No Hit CATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAAC 9 0.22499999999999998 No Hit GTCCGCAGTTGGCCAAACGCATTCAGACAAATGCATATCGGTCCCCTTGC 8 0.2 No Hit CCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATA 8 0.2 No Hit AGTATTTTCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACT 8 0.2 No Hit CAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCC 8 0.2 No Hit GGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGC 8 0.2 No Hit GTGGCACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACC 8 0.2 No Hit GGACCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCACAGA 8 0.2 No Hit GTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCACAGAGGGGAT 8 0.2 No Hit CAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAATTC 8 0.2 No Hit GCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAG 8 0.2 No Hit GGAAAATTGTCAGTATTTCCAAATATAAATATAGAAGAAAATAATGGTAA 8 0.2 No Hit GTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTG 8 0.2 No Hit GGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATA 8 0.2 No Hit CCAGGATTTGTTGTTTTGTGCGATCCACGAGGGATCCAGCTTGCTTGTCC 8 0.2 No Hit ACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGT 8 0.2 No Hit CCTCACAGAAACAACAGTCCCAAGAAAAATTCGTCCACTTCAAGGCTTTG 8 0.2 No Hit GCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGCCAAA 7 0.17500000000000002 No Hit TTTTTTTTTTGGATCAACGAACCATGTATATTACTAATCTTTACAACAGC 7 0.17500000000000002 No Hit ATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAG 7 0.17500000000000002 No Hit CTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAA 7 0.17500000000000002 No Hit CCTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTT 7 0.17500000000000002 No Hit GCCCTTAAAATGTTTTAGAACATGAACACTTGTAGGAGATGATTGGATGG 7 0.17500000000000002 No Hit CCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGA 7 0.17500000000000002 No Hit GTCTGAAACATTGCATGTCAAGAATCTAGGCTAATAGAATTAGGAGCCCT 7 0.17500000000000002 No Hit GGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATC 7 0.17500000000000002 No Hit CCCTGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 7 0.17500000000000002 No Hit CTTCTTTTCATGCTAATTTTTGGCAAACCGTTGGTCTCATCTTTTTGGGT 7 0.17500000000000002 No Hit CGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGT 7 0.17500000000000002 No Hit CTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCTTGTGC 7 0.17500000000000002 No Hit AGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTC 7 0.17500000000000002 No Hit CCTTAAAATGTTTTAGAACATGAACACTTGTAGGAGATGATTGGATGGAA 7 0.17500000000000002 No Hit CTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTT 7 0.17500000000000002 No Hit ATCAGGTACAATTTTTTTCTCCCACTCAAAGCTCGACCTCAAACCTCCAC 7 0.17500000000000002 No Hit CTTGGATATGGAGGAACTGTTAATCATGATTAGATTTTTTTTCAAAAAGG 7 0.17500000000000002 No Hit CCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTT 6 0.15 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTT 6 0.15 No Hit ATCAGGTCAACCTAACAGTCTGAAACATTGCATGTCAAGAATCTAGGCTA 6 0.15 No Hit CCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCC 6 0.15 No Hit GTCCGGAAGCCGCACCATTGCCACTGCCATTGGCGCCAGTGCTTCCCAAA 6 0.15 No Hit AAGCTTGCACACTCGACGTCCTTCTTTTCATGCTAATTTTTGGCAAACCG 6 0.15 No Hit CTCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 6 0.15 No Hit CACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATAA 6 0.15 No Hit GCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAACACGGCGTCT 6 0.15 No Hit CGGCACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCT 6 0.15 No Hit CACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACG 6 0.15 No Hit GGTCAACCTAACAGTCTGAAACATTGCATGTCAAGAATCTAGGCTAATAG 6 0.15 No Hit CTGTGGACCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCA 6 0.15 No Hit TCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGC 6 0.15 No Hit GTATTTTCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTT 6 0.15 No Hit GCTCAAGTTAGTGAAGCTTTCTCATACAACTCTAGCAAGAAATTGCCTAG 6 0.15 No Hit ACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCG 6 0.15 No Hit GGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGC 6 0.15 No Hit ATCCAAGCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGG 6 0.15 No Hit CCCTTAAAATGTTTTAGAACATGAACACTTGTAGGAGATGATTGGATGGA 6 0.15 No Hit CAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCA 6 0.15 No Hit GCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAACACTGCGTCT 6 0.15 No Hit GCTTGAGATAATTGAGGTGTTCAGTTCACATTGCTACCCTAGTTAGAAAA 6 0.15 No Hit CTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTT 6 0.15 No Hit GTTCCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGC 6 0.15 No Hit GGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACC 6 0.15 No Hit TTTTTTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTC 6 0.15 No Hit CCCACCGCAAAAGCATGTTAAATAGCAAGGCACATTGCCAAAAAAAAATA 6 0.15 No Hit GGCCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTC 6 0.15 No Hit GCCTTTTTTCTTTTTTTGCTTCAGACGTCTTCGTCCATGCCGTCCTCCTC 6 0.15 No Hit AACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTG 6 0.15 No Hit CATGTCAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATA 6 0.15 No Hit GTTGCAAATAATGGACGCCCATAATTGGGCAAAGGAAAAGAAAAGGCTCT 6 0.15 No Hit CTACAGAAAATTATCGATTTGTTTGAGATCTACAAGATTAGAACTTTGTT 6 0.15 No Hit CCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGC 6 0.15 No Hit GGTTCTACTCTGAAATTGCCAACCTTTTCTCTAGTGCCATCGACTACTGC 6 0.15 No Hit GCACACTCGACGTCCTTCTTTTCATGCTAATTTTTGGCAAACCGTTGGTC 6 0.15 No Hit GTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAA 6 0.15 No Hit GCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCG 6 0.15 No Hit CTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTA 5 0.125 No Hit CCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCTGACAAATCTTTC 5 0.125 No Hit CCCCTCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 5 0.125 No Hit CTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACA 5 0.125 No Hit CACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCTTGT 5 0.125 No Hit ATGTTATTGAATCTTCAATAGCATTTTTTATAGTGTCCTCGAGAGCATCT 5 0.125 No Hit ACAGAAAATTATCGATTTGTTTGAGATCTACAAGATTAGAACTTTGTTCT 5 0.125 No Hit CTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGA 5 0.125 No Hit TCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAACATGCACGTC 5 0.125 No Hit GAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCA 5 0.125 No Hit GCCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTT 5 0.125 No Hit GGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAAT 5 0.125 No Hit TTTTTTTTTTGAAAGGCGAAGGCATCACCAAGATATACTATTTCTGTCAG 5 0.125 No Hit CCCCTGCAACTTGCAAGTTTGCTCATTCTCTGTAGTGCACATGCATTGCG 5 0.125 No Hit GGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTA 5 0.125 No Hit GCCATTTTAGAGTTTTATTATTCTATCTATATAATACTTGTTTATGTTTG 5 0.125 No Hit AGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAAT 5 0.125 No Hit CCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAA 5 0.125 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTT 5 0.125 No Hit CAGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACT 5 0.125 No Hit CATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGT 5 0.125 No Hit CCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTTTTCATGCCGCC 5 0.125 No Hit GGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATA 5 0.125 No Hit CACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACATTCCC 5 0.125 No Hit CCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCA 5 0.125 No Hit GATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAGG 5 0.125 No Hit GGACGGAATGGCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCC 5 0.125 No Hit CAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCCCATCCTTTCTAT 5 0.125 No Hit GCCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCG 5 0.125 No Hit GCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 5 0.125 No Hit ACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTT 5 0.125 No Hit AACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCC 5 0.125 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 5 0.125 No Hit CCGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGT 5 0.125 No Hit CCTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCG 5 0.125 No Hit CACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAA 5 0.125 No Hit CCCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACA 5 0.125 No Hit GCCATTTTTCTGGCCTCACTGGAAATCACGCATCGACACGACAGGGCACG 5 0.125 No Hit CTACTGCTTACATCTAAATCTGAATATGCCTCCTTTTTGGTTAAAAATGG 5 0.125 No Hit TGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAA 5 0.125 No Hit GCCCACATGAACTTCTCCTCCTGTTTCAATTTCTCTTCCCGCAATGCTTT 5 0.125 No Hit GGGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTGGCGCGATGACAA 5 0.125 No Hit TTCTTCCCGCAATGCTTTCTTCTCCTCCGATGTCATCTGTTTTTTTCTTC 5 0.125 No Hit GGACTTGATAAGACACATTCCCAACTTCAAGAGTTCTCTTAATAAGCTCC 5 0.125 No Hit TGAGAAGTGGCACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTT 5 0.125 No Hit GGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTC 5 0.125 No Hit CCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGACC 5 0.125 No Hit GGCAAGGTAACTCTATTACTCAAGAACGTACACGAATACTGAGTGAAAAA 5 0.125 No Hit CTCCATTTTTCAAGACCACTTCCCTTGGAAGACTTAGGTTTACCTAGAAG 5 0.125 No Hit CGGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTG 5 0.125 No Hit GGAACCTCGTTTTCGAAGAAAAAATGGTTGAGATGGTTGGGGATCTCTGT 5 0.125 No Hit GCAAGGAAAATTGTCAGTATTTCCAAATATAAATATAGAAGAAAATAATG 5 0.125 No Hit GATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATC 5 0.125 No Hit CCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTAAA 10 0.0071878894 142.55 1 TACCCCC 20 0.00645042 28.51 10-14 >>END_MODULE ERR5262782 read2 length is 81-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262782_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 81-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.095 37.0 37.0 37.0 37.0 37.0 2 35.907 37.0 37.0 37.0 37.0 37.0 3 35.87 37.0 37.0 37.0 37.0 37.0 4 35.991 37.0 37.0 37.0 37.0 37.0 5 36.1355 37.0 37.0 37.0 37.0 37.0 6 36.136 37.0 37.0 37.0 37.0 37.0 7 36.1305 37.0 37.0 37.0 37.0 37.0 8 36.0965 37.0 37.0 37.0 37.0 37.0 9 36.126 37.0 37.0 37.0 37.0 37.0 10-14 36.16969999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.147400000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.135400000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.065599999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.001099999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.0279 37.0 37.0 37.0 37.0 37.0 40-44 36.0087 37.0 37.0 37.0 37.0 37.0 45-49 35.9976 37.0 37.0 37.0 37.0 37.0 50-54 35.9913 37.0 37.0 37.0 37.0 37.0 55-59 35.9042 37.0 37.0 37.0 37.0 37.0 60-64 35.865300000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.943200000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.82719999999999 37.0 37.0 37.0 37.0 37.0 75-79 35.8186 37.0 37.0 37.0 37.0 37.0 80-84 35.74200897724431 37.0 37.0 37.0 37.0 37.0 85-89 35.71766379678109 37.0 37.0 37.0 37.0 37.0 90-94 35.69697121401752 37.0 37.0 37.0 37.0 37.0 95-99 35.606646609032005 37.0 37.0 37.0 37.0 37.0 100-104 35.64506687622016 37.0 37.0 37.0 37.0 37.0 105-109 35.44038843777373 37.0 37.0 37.0 34.6 37.0 110-114 35.48307667773442 37.0 37.0 37.0 37.0 37.0 115-119 35.49272267338611 37.0 37.0 37.0 37.0 37.0 120-124 35.45947229486956 37.0 37.0 37.0 37.0 37.0 125-129 35.44532790032264 37.0 37.0 37.0 34.6 37.0 130-134 35.35368312847446 37.0 37.0 37.0 34.6 37.0 135-139 35.27471357247813 37.0 37.0 37.0 29.8 37.0 140-144 35.20950121043743 37.0 37.0 37.0 27.4 37.0 145-149 35.191236478370776 37.0 37.0 37.0 27.4 37.0 150 35.04359823399559 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 1.0 13 2.0 14 0.0 15 0.0 16 1.0 17 1.0 18 2.0 19 1.0 20 2.0 21 6.0 22 5.0 23 4.0 24 5.0 25 9.0 26 10.0 27 9.0 28 20.0 29 11.0 30 32.0 31 46.0 32 70.0 33 115.0 34 254.0 35 696.0 36 2486.0 37 210.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.75 21.85 10.65 25.75 2 32.125 24.0 27.625 16.25 3 21.625 25.75 32.775 19.85 4 26.6 27.450000000000003 23.175 22.775000000000002 5 26.700000000000003 33.0 21.2 19.1 6 23.799999999999997 34.8 21.45 19.950000000000003 7 22.075 19.35 37.824999999999996 20.75 8 19.375 22.525000000000002 28.875 29.225 9 22.1 21.0 31.275 25.624999999999996 10-14 25.235000000000003 26.115 25.569999999999997 23.080000000000002 15-19 24.425 25.035 26.575 23.965 20-24 25.590000000000003 25.305 26.165 22.939999999999998 25-29 24.335 25.47 27.139999999999997 23.055 30-34 25.515 26.090000000000003 26.255 22.14 35-39 24.27 26.340000000000003 27.060000000000002 22.33 40-44 25.695 25.035 26.334999999999997 22.935 45-49 24.89 24.77 27.725 22.615 50-54 24.095 25.085 27.495000000000005 23.325000000000003 55-59 23.865 26.72 26.075 23.34 60-64 24.19 25.35 27.439999999999998 23.02 65-69 24.595 25.095 27.229999999999997 23.080000000000002 70-74 23.645 25.509999999999998 27.61 23.235 75-79 24.0 24.79 28.015 23.195 80-84 24.058608791318697 26.628994349152375 27.434115117267588 21.878281742261336 85-89 23.036518259129565 26.558279139569784 28.33416708354177 22.07103551775888 90-94 23.819774718397998 27.46433041301627 27.219023779724655 21.496871088861077 95-99 24.613150383093796 25.54960188291852 27.843156893184435 21.994090840803246 100-104 25.525194284281778 24.8784156430183 27.08448232639759 22.51190774630233 105-109 24.27457882826251 26.301232084485793 27.97586120191099 21.44832788534071 110-114 23.83565782186194 26.297769498011174 28.72967121494386 21.136901465183023 115-119 23.82828282828283 26.626262626262626 28.075757575757578 21.46969696969697 120-124 25.427860443857597 24.31567721293992 28.454623939871006 21.801838403331473 125-129 25.2112892485786 26.123034369717768 27.55724017825129 21.10843620345234 130-134 24.662933001451982 25.30595312175897 28.142501555693837 21.88861232109521 135-139 24.548224416894303 26.744063878966166 26.71779785669258 21.989913847446942 140-144 23.366389707853124 27.397480568212274 27.5797373358349 21.656392388099704 145-149 24.656263640331733 26.46769969445657 27.673505019642075 21.202531645569618 150 24.530905077262695 24.420529801324502 30.518763796909493 20.52980132450331 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.5 20 0.5 21 0.5 22 0.5 23 1.0 24 2.0 25 1.0 26 2.0 27 4.0 28 3.5 29 2.5 30 2.5 31 7.0 32 11.0 33 17.0 34 30.5 35 41.5 36 82.0 37 100.0 38 123.0 39 173.0 40 163.5 41 167.0 42 173.0 43 188.0 44 293.0 45 287.0 46 199.0 47 192.5 48 196.5 49 165.0 50 125.0 51 119.0 52 108.5 53 95.5 54 79.5 55 65.5 56 92.0 57 101.0 58 90.5 59 90.0 60 60.5 61 40.0 62 42.5 63 38.0 64 24.5 65 18.5 66 17.0 67 17.5 68 32.0 69 34.5 70 19.5 71 10.5 72 9.5 73 9.0 74 7.5 75 7.0 76 4.5 77 3.5 78 2.5 79 1.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 80-81 1.0 82-83 0.0 84-85 0.0 86-87 2.0 88-89 2.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 0.0 98-99 3.0 100-101 1.0 102-103 5.0 104-105 10.0 106-107 0.0 108-109 1.0 110-111 1.0 112-113 4.0 114-115 7.0 116-117 3.0 118-119 10.0 120-121 11.0 122-123 12.0 124-125 14.0 126-127 16.0 128-129 18.0 130-131 22.0 132-133 23.0 134-135 14.0 136-137 18.0 138-139 45.0 140-141 22.0 142-143 29.0 144-145 37.0 146-147 8.0 148-149 36.0 150-151 3624.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.5 #Duplication Level Percentage of deduplicated Percentage of total 1 51.61290322580645 24.0 2 22.849462365591396 21.25 3 10.21505376344086 14.249999999999998 4 6.989247311827956 13.0 5 3.118279569892473 7.249999999999999 6 1.6666666666666667 4.65 7 1.021505376344086 3.325 8 0.6451612903225806 2.4 9 0.5376344086021506 2.25 >10 1.3440860215053763 7.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCG 17 0.42500000000000004 No Hit GCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAG 16 0.4 No Hit AACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGA 15 0.375 No Hit GGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGC 15 0.375 No Hit GGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAAC 15 0.375 No Hit GTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCC 15 0.375 No Hit GGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGG 13 0.325 No Hit GTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTT 13 0.325 No Hit AGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAG 13 0.325 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 12 0.3 No Hit CATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGC 12 0.3 No Hit GATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAAACCCTTTGA 12 0.3 No Hit GGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAG 12 0.3 No Hit CTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAAG 11 0.27499999999999997 No Hit AGGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCG 11 0.27499999999999997 No Hit GCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGT 11 0.27499999999999997 No Hit GTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGA 11 0.27499999999999997 No Hit GACAAACTAGAGCACCCAAGGTTATTGAAGTGTTGCGGGGGAGACGGTCT 11 0.27499999999999997 No Hit GGGGAACGGAAGCGCCGACCAGAACCGAGGGCGAGCGGCGGTAGTTCTCC 10 0.25 No Hit GAAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAA 10 0.25 No Hit GTGAATTTGTGCAGCTCTTCATCAAGTTTACACAGAATCTCGCACTCTTC 10 0.25 No Hit GTCGTCGATCTCGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCC 10 0.25 No Hit CGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGAGGGGT 10 0.25 No Hit GAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTATTGAA 10 0.25 No Hit CTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCCTTTCCAGATGCAT 10 0.25 No Hit GAGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCG 9 0.22499999999999998 No Hit GGGTGCTCTCGAAAAAACAAAGGTTATTTGAAGGTTGGCATGCCCTTTGG 9 0.22499999999999998 No Hit ATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTGCTC 9 0.22499999999999998 No Hit GATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAG 9 0.22499999999999998 No Hit GGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTG 9 0.22499999999999998 No Hit CTGATGAGGACGGGGATGTTGTCATGCTGGATGATGACGAAGACTTGCAT 9 0.22499999999999998 No Hit GTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCG 9 0.22499999999999998 No Hit GGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTC 9 0.22499999999999998 No Hit GTTTCTTCTACCCAGATGTATTTGCAACCCTGAGACTGAATGCAATGGAA 9 0.22499999999999998 No Hit GACGAAGACTTGCATGATGCAGCTATCAAGCAGGAGCTGAATCCTCTGAG 9 0.22499999999999998 No Hit CTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTT 8 0.2 No Hit TGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTT 8 0.2 No Hit GGACTGCTGTGTAACACATCTTGTCGTTTCGAACGGGAAGTAGATCGAAA 8 0.2 No Hit CAATAAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTC 8 0.2 No Hit TACAAATCAAGAGAAAAAGGGGGCCAGAAAAAACTTTCGTCTCTTGTCTA 8 0.2 No Hit GTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTAT 8 0.2 No Hit GCCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGA 8 0.2 No Hit GCACAAGCTTCAACAACAGTACTGCAATTCAGCGAGGCCTTATGGCTCTC 8 0.2 No Hit CCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAA 8 0.2 No Hit CTTGCCAAAGATTATTCCATTTTACCCGGGAACCAAAGATGACAAGAACG 8 0.2 No Hit GGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTT 8 0.2 No Hit AGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATCC 8 0.2 No Hit CATAGACTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAACAACATG 7 0.17500000000000002 No Hit GGAGAAGAGTGAATTTGTGCAGCTCTTCATCAAGTTTACACAGAATCTCG 7 0.17500000000000002 No Hit AGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCA 7 0.17500000000000002 No Hit TATAGCGAGGATGGGGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCA 7 0.17500000000000002 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 7 0.17500000000000002 No Hit GTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATT 7 0.17500000000000002 No Hit ATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTGC 7 0.17500000000000002 No Hit TGGATGGACAGAGATTCTGCAGGAACAGCTGGAAGGCAAAAAAACTGAAA 7 0.17500000000000002 No Hit GGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCAT 7 0.17500000000000002 No Hit ACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGCCGTGGCAT 7 0.17500000000000002 No Hit CGGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTA 7 0.17500000000000002 No Hit TGATTAATACGGCGGCCGCGTCGTCGATCTCGCTGCAAGCCTGCAAGCTA 7 0.17500000000000002 No Hit CTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACT 7 0.17500000000000002 No Hit AGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTA 7 0.17500000000000002 No Hit GTAATTTTGAGTGGGTGCGCCATTTGGAAGATGGCTCCGTAAAATTCATA 7 0.17500000000000002 No Hit GATCAAGGCCGACACGCCGTGGCATTATAGCGAGGATGGGGTGGATCTTG 7 0.17500000000000002 No Hit CTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGC 7 0.17500000000000002 No Hit AGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCT 7 0.17500000000000002 No Hit GATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTG 7 0.17500000000000002 No Hit ACCTGTTGAGCTAACTCCAGCACAGGAGGAGGTGGCAACCATGTTTGCTG 6 0.15 No Hit CATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAG 6 0.15 No Hit AGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTCC 6 0.15 No Hit CGGGGATGTTGTCATGCTGGATGATGACGAAGACTTGCATGATGCAGCTA 6 0.15 No Hit GCTTCATGAACTATATAAGGTAAATGTGTGCCATTCAAGCTAGTATTCCA 6 0.15 No Hit ATCGGGCGTGCGAGCGCGGGACCTTGTCCTTATCTTGTACGTGCCCCGGC 6 0.15 No Hit GCTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAA 6 0.15 No Hit GTTTATCTTCAGCAGTTCATTCGGTGCTTATCTCCTGGCGCTCTACCTTG 6 0.15 No Hit CGCTTCGTGATCGATAGGGAGGAGTGCTTCTCGCATAACGTGGAGTACGA 6 0.15 No Hit TGCAGCTATCAAGCAGGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAA 6 0.15 No Hit CATCAAGCAGTGATACTGCAGTTATTATGTATACAAGTGGAAGTACTGGT 6 0.15 No Hit GTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGC 6 0.15 No Hit GGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTT 6 0.15 No Hit CTGTGGTCTATGACTTCTACAACTACGACATGGAGAAGAGTGAATTTGTG 6 0.15 No Hit AGGGTCTGGGAGGTCTCCTGTTTATCTTCAGCAGTTCATTCGGTGCTTAT 6 0.15 No Hit CGAGAAGAGGGTGGTGACGCAGCTGTCCAAGATGCTGATCCAGGAGGAGA 6 0.15 No Hit ATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAA 6 0.15 No Hit CAACCAGATAGTCGAGCTGCTATCTTTTCTGATACAAGAGCTGAGTGGAT 6 0.15 No Hit CTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTGCCC 6 0.15 No Hit ATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCA 6 0.15 No Hit CATGCCCTTTGGCCTGTTGCCATCGGTGTGTATATCACTAGTTTTTGTTT 6 0.15 No Hit GAACGGCGTGCCTCGTGGTCCTCTGTGTGCTGCCCTTCTTGCGCCCGGCT 6 0.15 No Hit CTATCAAGCAGGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGAAC 6 0.15 No Hit CAACTCTTGCTGACTTGGGTGTGACGCTGAGCAATGCAGCTTACAAGCTT 6 0.15 No Hit ATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAA 6 0.15 No Hit CAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATTGT 6 0.15 No Hit CGACTATTCTACTCCACTCTACTCCTGCACGCATGCTCGCTCGTTGATGG 6 0.15 No Hit AACACATTTCACTCTGAATTTATCTGCTCTTCGGTCCAAAATTGCAAGTG 6 0.15 No Hit GGATTGACTATTACTTGGAGTATGAACCTCATCCATCATCAACTAAAAAC 6 0.15 No Hit GGGAATCCTATGGGTTTTGCCAGGAATGTTGGGTTTGGTTTAAAAGACTT 6 0.15 No Hit CGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTT 6 0.15 No Hit GTGGAGTACGAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGC 5 0.125 No Hit GAAGCTTTAAAACCCACACCAAACCCTTTGAGACCCAGTACAATTGAAAA 5 0.125 No Hit ATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCC 5 0.125 No Hit ATGAAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCC 5 0.125 No Hit TAGGGCCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTG 5 0.125 No Hit AGATGGTTATGCCCGGTGACAACGTAACTGCGATTTTTGAATTGATATCA 5 0.125 No Hit GAATTAATGGATGGCTAGTCCTTGATTTATTTTCTTCCTTGGTAAAGCTA 5 0.125 No Hit GGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTC 5 0.125 No Hit AGCTGGCAAGAGCTTGCTGAATATGACCTTATGGCCATGTTAAGCTATGT 5 0.125 No Hit GCTGAACTTTCGGAGTGATTTGGGGGTTCAAATATTAGATTCTCTGTGTG 5 0.125 No Hit CAAAAATCGTTCTGTAAATCAACCCAACCCGATTCCTAATCTGATTTCGA 5 0.125 No Hit GTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTT 5 0.125 No Hit CCATGATGATGCTCAGCTTGTTGTAACAGATGAAGAGCATAAAATGCCTC 5 0.125 No Hit ACTAATTGCTCCATTTTCCGTTTCTGTGACTTTGTTTGTTGATGTAAGAT 5 0.125 No Hit AGATCGAGAAGGAGAAGAGTCATGGTCACCTGCATCAAAAGGATGACCAA 5 0.125 No Hit AGGGGCTGGGAGAGAGCCTCGGGGGAAGGATCAAGAGAGGCTCGCCCGCG 5 0.125 No Hit TGCATCTTTTCTTATGCATGAGGGTGCTGAGCTATGGACTGTTCGCGTTA 5 0.125 No Hit ATTAATGTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCA 5 0.125 No Hit GAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACC 5 0.125 No Hit CATGAATCCTGGTAGGCGATTTGTCTCTCTTTACTGCTCTGCACTGTTAT 5 0.125 No Hit CGATCTCGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCCTTTCC 5 0.125 No Hit GATTACCATCAAGCAGTGATACCGCAGTTATTATGTATACAAGTGGAAGT 5 0.125 No Hit GATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACT 5 0.125 No Hit GCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCAT 5 0.125 No Hit CGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACT 5 0.125 No Hit AGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAAG 5 0.125 No Hit ACGGTATGCCAAGGATTATCCCATTTTACCCGGAAACCAAAGATGACAAA 5 0.125 No Hit GCCGACACGCCGTGGCATTATAGCGAGGATGGGGTGGATCTTGTTGTTAA 5 0.125 No Hit CTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTCGTGACAA 5 0.125 No Hit CAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGC 5 0.125 No Hit GGAAACCAAGGAACCATCATCCGCCAAGTCACCAACCAGGAGCAATATTG 5 0.125 No Hit ATTGCAAGTGCTTTTAAGTTCGCCCTGGATGCTGACTTCATTCTGACTTA 5 0.125 No Hit TGTCAAGATGTCGCCCTCGGGTGCTTTGGAAAGATCGCCAAGCTTGTCTA 5 0.125 No Hit TGTTTATCTTCAGCAGTTCATTCGGTGCTTATCTCCTGGCGCTCTACCTT 5 0.125 No Hit CTCTGGCTAACGTTTCAGATTCTAGAATGCCGAAAAAGCATTGCTTTCAT 5 0.125 No Hit GTCATCGCTAAAGTTATGAGCGAATAATCTGTTACGAACTTTTTTTTGGC 5 0.125 No Hit TGTTAGTGCAACTTTTGTTCTCAGAGCAGTCGGCATCCATCTTGACCAGA 5 0.125 No Hit TTTTCATATTTCGAACAACATGCCAAAGATGAACATTTTGCACCTCTGTT 5 0.125 No Hit CAAGGCCGACACGCCGTGGCATTATAGCGAGGATGGGGTGGATCTTGTTG 5 0.125 No Hit GCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAAC 5 0.125 No Hit TCGCAAATGGATGGCACAATGGAGACCACCCTGCTTCACCCTGGATGATG 5 0.125 No Hit CAATAGATGGATTCCAGGAGTCTTCAGTGACTTCTGCCATGCCAAAAAAA 5 0.125 No Hit TAGCGAGGATGGGGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAG 5 0.125 No Hit GTTATTGATGAAGCTTTAAAACCCACACCAAACCCTTTGAGACCCAGTAC 5 0.125 No Hit AAACCCTTTGAGACCCAGTACAATTGAAAAAGATCCACTGGCTCATTTGA 5 0.125 No Hit GTTGAGCTAACTCCAGCACAGGAGGAGGTGGCAACCATGTTTGCTGTGAT 5 0.125 No Hit ATCTGCCCATCTGGGAGTTGTGGTGCCCCATGTTGAGTTGAAGCATGTGA 5 0.125 No Hit AGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGT 5 0.125 No Hit AAGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAA 5 0.125 No Hit TGAATGTTAGGGGGCTCAGCATTACTCATGTGAAAAGCCATTTGCAGGTA 5 0.125 No Hit TGGGGGTTCAAATATTAGATTCTCTGTGTGATGATGGACATCTGGACTGC 5 0.125 No Hit TGGGATGCCAATCCAAGTATCATATTGGATGTAAAAACTAGACTTGGGTT 5 0.125 No Hit GTCGGGTTGCAGGGGTCAAATCTCCAGAGGGTCATCATGAATTTCCCTGG 5 0.125 No Hit CCCAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTG 5 0.125 No Hit CTATGACTTCTACAACTACGACATGGAGAAGAGTGAATTTGTGCAGCTCT 5 0.125 No Hit GATCAATGGAGTGCCAATTCGCCGCGTCAACCAGACTTATGTTATTGCCA 5 0.125 No Hit GAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGCC 5 0.125 No Hit CTCGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGAGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0125 62-63 0.0 0.0 0.0 0.0 0.025 64-65 0.0 0.0 0.0 0.0 0.025 66-67 0.0 0.0 0.0 0.0 0.025 68-69 0.0 0.0 0.0 0.0 0.025 70-71 0.0 0.0 0.0 0.0 0.025 72-73 0.0 0.0 0.0 0.0 0.025 74-75 0.0 0.0 0.0 0.0 0.025 76-77 0.0 0.0 0.0 0.0 0.025 78-79 0.0 0.0 0.0 0.0 0.025 80-81 0.0125 0.0 0.0 0.0 0.025 82-83 0.025 0.0 0.0 0.0 0.025 84-85 0.025 0.0 0.0 0.0 0.025 86-87 0.025 0.0 0.0 0.0 0.025 88-89 0.025 0.0 0.0 0.0 0.025 90-91 0.025 0.0 0.0 0.0 0.025 92-93 0.025 0.0 0.0 0.0 0.025 94-95 0.025 0.0 0.0 0.0 0.025 96-97 0.025 0.0 0.0 0.0 0.025 98-99 0.025 0.0 0.0 0.0 0.025 100-101 0.025 0.0 0.0 0.0 0.025 102-103 0.025 0.0 0.0 0.0 0.025 104-105 0.025 0.0 0.0 0.0 0.025 106-107 0.025 0.0 0.0 0.0 0.025 108-109 0.025 0.0 0.0 0.0 0.025 110-111 0.025 0.0 0.0 0.0 0.025 112-113 0.025 0.0 0.0 0.0 0.025 114-115 0.025 0.0 0.0 0.0 0.025 116-117 0.025 0.0 0.0 0.0 0.025 118-119 0.025 0.0 0.0 0.0 0.025 120-121 0.025 0.0 0.0 0.0 0.025 122-123 0.025 0.0 0.0 0.0 0.025 124-125 0.025 0.0 0.0 0.0 0.025 126-127 0.025 0.0 0.0 0.0 0.025 128-129 0.025 0.0 0.0 0.0 0.025 130-131 0.025 0.0 0.0 0.0 0.025 132-133 0.025 0.0 0.0 0.0 0.025 134-135 0.025 0.0 0.0 0.0 0.025 136-137 0.025 0.0 0.0 0.0 0.025 138 0.025 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCTC 10 0.0071973195 142.4875 2 CGCTCCG 10 0.0071973195 142.4875 4 CGCCAAC 10 0.0071973195 142.4875 9 TCGCTCC 10 0.0071973195 142.4875 3 GCTCCGC 10 0.0071973195 142.4875 5 GTTCGCT 10 0.0071973195 142.4875 1 >>END_MODULE Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832985 spots for ERR5262782.sra Written 1832985 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra Read 1832978 spots for ERR5262782.sra Written 1832978 spots for ERR5262782.sra SRR ids: ['ERR5262782.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_v1wqfy8c ERR5262782.sra spots: 36659567 blocks: [[1, 1832978], [1832979, 3665956], [3665957, 5498934], [5498935, 7331912], [7331913, 9164890], [9164891, 10997868], [10997869, 12830846], [12830847, 14663824], [14663825, 16496802], [16496803, 18329780], [18329781, 20162758], [20162759, 21995736], [21995737, 23828714], [23828715, 25661692], [25661693, 27494670], [27494671, 29327648], [29327649, 31160626], [31160627, 32993604], [32993605, 34826582], [34826583, 36659567]] ERR5262782 file size 12069772 ERR5262782 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262782 ERR5262782_1.fastq ERR5262782_2.fastq Input file: ERR5262782_1.fastq Paired file: ERR5262782_2.fastq trimmed: ERR5262782-trimmed-pair1.fastq, ERR5262782-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:19:07 2024 >> started Fri Dec 6 11:19:52 2024 >> done (44.233s) 36659567 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 36659566 (100.00%) read pairs available; of these: 9779 ( 0.03%) trimmed read pairs available after processing 36649787 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 22 1 0.00% 23 1 0.00% 24 2 0.00% 25 2 0.00% 26 1 0.00% 27 0 0.00% 28 2 0.00% 29 7 0.00% 30 1 0.00% 31 6 0.00% 32 6 0.00% 33 4 0.00% 34 4 0.00% 35 2 0.00% 36 4 0.00% 37 8 0.00% 38 4 0.00% 39 9 0.00% 40 5 0.00% 41 4 0.00% 42 3 0.00% 43 4 0.00% 44 3 0.00% 45 5 0.00% 46 6 0.00% 47 2 0.00% 48 5 0.00% 49 340 0.00% 50 361 0.00% 51 404 0.00% 52 493 0.00% 53 451 0.00% 54 516 0.00% 55 608 0.00% 56 618 0.00% 57 638 0.00% 58 757 0.00% 59 886 0.00% 60 1036 0.00% 61 1162 0.00% 62 1301 0.00% 63 1437 0.00% 64 1547 0.00% 65 1751 0.00% 66 1859 0.01% 67 2111 0.01% 68 2338 0.01% 69 2771 0.01% 70 3301 0.01% 71 3838 0.01% 72 4034 0.01% 73 4745 0.01% 74 4982 0.01% 75 5855 0.02% 76 6274 0.02% 77 6832 0.02% 78 7641 0.02% 79 8699 0.02% 80 9569 0.03% 81 10613 0.03% 82 12065 0.03% 83 13553 0.04% 84 14780 0.04% 85 15958 0.04% 86 17036 0.05% 87 18568 0.05% 88 19570 0.05% 89 21392 0.06% 90 22665 0.06% 91 25040 0.07% 92 27132 0.07% 93 29382 0.08% 94 31325 0.09% 95 33284 0.09% 96 35020 0.10% 97 37114 0.10% 98 38107 0.10% 99 39845 0.11% 100 41613 0.11% 101 43607 0.12% 102 46295 0.13% 103 48871 0.13% 104 52546 0.14% 105 54586 0.15% 106 57061 0.16% 107 57798 0.16% 108 59753 0.16% 109 61668 0.17% 110 63066 0.17% 111 65484 0.18% 112 68558 0.19% 113 71059 0.19% 114 74563 0.20% 115 76702 0.21% 116 78256 0.21% 117 81401 0.22% 118 82628 0.23% 119 82615 0.23% 120 84865 0.23% 121 87502 0.24% 122 89221 0.24% 123 91609 0.25% 124 95429 0.26% 125 96763 0.26% 126 99657 0.27% 127 101897 0.28% 128 103507 0.28% 129 105200 0.29% 130 105509 0.29% 131 106983 0.29% 132 109020 0.30% 133 110979 0.30% 134 113489 0.31% 135 117160 0.32% 136 119712 0.33% 137 119895 0.33% 138 122356 0.33% 139 124223 0.34% 140 126410 0.34% 141 131858 0.36% 142 134160 0.37% 143 136309 0.37% 144 138192 0.38% 145 136852 0.37% 146 142089 0.39% 147 289288 0.79% 148 132144 0.36% 149 131977 0.36% 150 31235476 85.20% 36659566 reads passed initial QC criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=2.14 fanout-score-rank=44 prefix-density=0.17 prefix-fanout=2.1 sequence=GATATCCACATC criterion=fanout-score sequence-density=0.04 sequence-density-rank=18 fanout-score=119.79 fanout-score-rank=1 prefix-density=0.63 prefix-fanout=7.7 sequence=CCTTCTTCTTCGTCTCCGGCGACGTCGTCTTGTCGGCGGCCTTGAGCTTGCGTGTGAGTGTGCGCCAGTAGTTCTTGATCTCGTTGTCGGTGCGGCCGGGGAGCCTCCGTGCGATGCGTGACCATCGGCTGCCCCACTGGGAGTGGAGCTGCAGGATGAGGCGCTCCTCGTCGGGCGTGATCCGGCCGCGCTTCAGCCCTGGGTGCAGGTAGTTCACCCACCGGAGACGGCAGCT criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.66 fanout-score-rank=34 prefix-density=0.37 prefix-fanout=2.4 sequence=ACCTTGTCAAGA criterion=fanout-score sequence-density=0.11 sequence-density-rank=14 fanout-score=111.89 fanout-score-rank=1 prefix-density=0.77 prefix-fanout=15.9 sequence=TGCTGCTGCTGCCGCGTCTGGTGCTGCAGCAGATTTAGTAAGGGACAGGGAACAGCTTCCTGACCGTGGATACCGTA ERR5262782 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:20:41 Started mapping on | Dec 06 11:20:41 Finished on | Dec 06 11:23:42 Mapping speed, Million of reads per hour | 729.14 Number of input reads | 36659566 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 35466092 Uniquely mapped reads % | 96.74% Average mapped length | 291.81 Number of splices: Total | 33667579 Number of splices: Annotated (sjdb) | 31303319 Number of splices: GT/AG | 33193426 Number of splices: GC/AG | 398843 Number of splices: AT/AC | 20684 Number of splices: Non-canonical | 54626 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.18 Insertion rate per base | 0.01% Insertion average length | 2.48 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 430799 % of reads mapped to multiple loci | 1.18% Number of reads mapped to too many loci | 831 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.07% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 762675 762675 762675 N_multimapping 430799 430799 430799 N_noFeature 1343313 34530593 1667253 N_ambiguous 721822 4684 110738 UnstrandedReadsAssigned:33400957 PositiveStrandReadsAssigned:930815 NegativeStrandReadsAssigned:33688101 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262782 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262782-trimmed-pair1.fastq ERR5262782-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 36,659,566 reads, 34,178,543 reads pseudoaligned [quant] estimated average fragment length: 274.546 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,199 rounds 52973 ERR5262782.ke.tsv 35125 ERR5262782.se.tsv 88098 total ==> ERR5262782.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 663.16 0 0 PNS24247 1044 770.454 150.846 8.00379 PNS24249 1928 1654.45 408.253 10.0875 PNS24246 1044 770.454 150.846 8.00379 PNS24248 1044 770.454 150.846 8.00379 PNS24244 1471 1197.45 226.209 7.72255 PNS24243 293 99.613 1 0.410386 KQK14069 1603 1329.45 67534.5 2076.64 KQK14071 474 233.967 388.532 67.8863 ==> ERR5262782.se.tsv <== BRADI_1g14170v3 68871 BRADI_1g53295v3 373 BRADI_1g59795v3 1009 BRADI_1g07683v3 0 BRADI_1g00485v3 44 BRADI_1g20270v3 1201 BRADI_1g74790v3 2845 BRADI_1g09890v3 0 BRADI_1g77505v3 512 BRADI_1g48960v3 0 ERR5262782 completed mapping pipeline successfully