Starting /dee2/code/volunteer_pipeline.sh ERR5262783 current disk space = 1551281147904 free memory = 1599265808 ERR5262783 SRAfilesize 440fb834704df62af3789b9a216492b0 ERR5262783.sra ERR5262783.sra file validated ERR5262783 is paired end ERR5262783 is conventional basespace ERR5262783 read1 length is 60-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262783_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 60-150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.557 37.0 37.0 37.0 37.0 37.0 2 36.389 37.0 37.0 37.0 37.0 37.0 3 36.661 37.0 37.0 37.0 37.0 37.0 4 36.68 37.0 37.0 37.0 37.0 37.0 5 36.7265 37.0 37.0 37.0 37.0 37.0 6 36.6275 37.0 37.0 37.0 37.0 37.0 7 36.6895 37.0 37.0 37.0 37.0 37.0 8 36.683 37.0 37.0 37.0 37.0 37.0 9 36.679 37.0 37.0 37.0 37.0 37.0 10-14 36.6964 37.0 37.0 37.0 37.0 37.0 15-19 36.6236 37.0 37.0 37.0 37.0 37.0 20-24 36.5689 37.0 37.0 37.0 37.0 37.0 25-29 36.52810000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.467600000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.4661 37.0 37.0 37.0 37.0 37.0 40-44 36.4058 37.0 37.0 37.0 37.0 37.0 45-49 36.3359 37.0 37.0 37.0 37.0 37.0 50-54 36.3462 37.0 37.0 37.0 37.0 37.0 55-59 36.3121 37.0 37.0 37.0 37.0 37.0 60-64 36.20263875968992 37.0 37.0 37.0 37.0 37.0 65-69 36.21750437609403 37.0 37.0 37.0 37.0 37.0 70-74 36.17039259814954 37.0 37.0 37.0 37.0 37.0 75-79 36.13198299574894 37.0 37.0 37.0 37.0 37.0 80-84 36.07864826031289 37.0 37.0 37.0 37.0 37.0 85-89 36.057668628872264 37.0 37.0 37.0 37.0 37.0 90-94 36.06088699574041 37.0 37.0 37.0 37.0 37.0 95-99 35.98724646974196 37.0 37.0 37.0 37.0 37.0 100-104 36.05509076316268 37.0 37.0 37.0 37.0 37.0 105-109 36.071488634425044 37.0 37.0 37.0 37.0 37.0 110-114 36.08317001136865 37.0 37.0 37.0 37.0 37.0 115-119 36.008275714610775 37.0 37.0 37.0 37.0 37.0 120-124 35.94834638682316 37.0 37.0 37.0 37.0 37.0 125-129 35.99081171066715 37.0 37.0 37.0 37.0 37.0 130-134 35.883519180382066 37.0 37.0 37.0 37.0 37.0 135-139 35.7830306149693 37.0 37.0 37.0 37.0 37.0 140-144 35.7519986615355 37.0 37.0 37.0 37.0 37.0 145-149 35.78148233991251 37.0 37.0 37.0 37.0 37.0 150 35.72418591706095 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 3.0 22 2.0 23 3.0 24 1.0 25 3.0 26 5.0 27 5.0 28 18.0 29 16.0 30 29.0 31 37.0 32 38.0 33 67.0 34 105.0 35 322.0 36 3028.0 37 316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.175000000000004 11.5 4.25 42.075 2 17.058529264632316 7.7788894447223615 40.8704352176088 34.29214607303652 3 17.349999999999998 13.5 26.450000000000003 42.699999999999996 4 18.099999999999998 23.925 25.275 32.7 5 21.3 28.675 25.0 25.025 6 17.65 37.65 21.85 22.85 7 12.675 28.975 38.7 19.650000000000002 8 12.775 28.15 27.700000000000003 31.374999999999996 9 14.025000000000002 24.15 33.4 28.425 10-14 19.27 26.655 24.404999999999998 29.67 15-19 19.355 28.76 22.36 29.525000000000002 20-24 19.41 25.505 25.215 29.87 25-29 20.025000000000002 27.71 26.68 25.585 30-34 19.24 26.27 25.874999999999996 28.615000000000002 35-39 17.630000000000003 27.08 27.950000000000003 27.339999999999996 40-44 17.9 29.92 25.924999999999997 26.255 45-49 17.76 31.34 26.625 24.275 50-54 19.285 28.335 29.325000000000003 23.055 55-59 20.119999999999997 32.73 26.66 20.49 60-64 17.358471694338867 34.961992398479694 25.60512102420484 22.074414882976594 65-69 16.65416354088522 33.00325081270318 30.17754438609652 20.16504126031508 70-74 19.56989247311828 32.713178294573645 24.391097774443608 23.325831457864467 75-79 20.94523630907727 32.18304576144036 23.845961490372595 23.025756439109777 80-84 20.646679012963613 30.487011361930026 27.268632063666846 21.597677561439514 85-89 21.339875826156618 31.053474864810738 24.579411175645905 23.02723813338674 90-94 23.061889250814332 31.821598596842897 24.164369832122276 20.952142320220496 95-99 18.557528337847327 30.303942220884743 29.466345671581905 21.672183769686026 100-104 18.860816716058064 30.604249334471845 28.836204731528454 21.698729217941633 105-109 22.193079794209623 29.55210329869868 24.836073842429133 23.418743064662564 110-114 19.915898267301653 27.88529739588611 28.00689026243794 24.191914074374303 115-119 21.54301459332585 27.85998571282784 26.237371160322482 24.35962853352383 120-124 22.636866926389175 28.003895837605086 24.871847447201148 24.48738978880459 125-129 20.415027944524944 28.286069136824675 28.182570896294763 23.11633202235562 130-134 19.95511013675749 29.481156696941223 27.534189372585864 23.02954379371542 135-139 18.21966902076526 31.33234953093707 27.81701275429535 22.63096869400232 140-144 23.031758740325596 26.410461702695493 28.55084067253803 22.006938884440885 145-149 20.111946441310433 28.030510892827742 25.374526697031225 26.483015968830596 150 19.343167269691065 29.501809073197883 28.054550514890064 23.100473142220984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 1.0 12 1.0 13 0.0 14 0.5 15 0.5 16 1.0 17 1.0 18 0.5 19 0.5 20 0.5 21 2.0 22 1.5 23 0.0 24 0.5 25 1.5 26 1.5 27 3.5 28 5.5 29 3.0 30 10.5 31 16.0 32 25.0 33 43.0 34 52.5 35 48.5 36 66.0 37 141.5 38 177.5 39 129.0 40 158.0 41 210.0 42 226.0 43 222.5 44 367.0 45 463.0 46 316.0 47 285.5 48 251.5 49 140.0 50 89.5 51 50.0 52 13.5 53 5.0 54 27.5 55 115.0 56 111.0 57 49.5 58 69.0 59 48.0 60 7.0 61 1.0 62 0.5 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 1.5 69 2.5 70 2.0 71 3.5 72 7.0 73 5.0 74 4.0 75 5.5 76 3.0 77 2.0 78 1.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 60-61 1.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 4.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 5.0 98-99 0.0 100-101 4.0 102-103 2.0 104-105 11.0 106-107 9.0 108-109 2.0 110-111 13.0 112-113 4.0 114-115 18.0 116-117 8.0 118-119 3.0 120-121 9.0 122-123 12.0 124-125 20.0 126-127 11.0 128-129 13.0 130-131 15.0 132-133 14.0 134-135 14.0 136-137 17.0 138-139 14.0 140-141 24.0 142-143 34.0 144-145 53.0 146-147 43.0 148-149 25.0 150-151 3593.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.775 #Duplication Level Percentage of deduplicated Percentage of total 1 38.346727898966705 8.35 2 15.269804822043628 6.65 3 11.940298507462686 7.8 4 7.577497129735936 6.6000000000000005 5 6.084959816303099 6.625 6 3.788748564867968 4.95 7 3.214695752009185 4.9 8 2.066590126291619 3.5999999999999996 9 1.2629161882893225 2.475 >10 9.644087256027554 36.025 >50 0.8036739380022963 12.025 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGG 95 2.375 No Hit GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 86 2.15 No Hit CCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 71 1.775 No Hit GTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAG 71 1.775 No Hit GTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 54 1.35 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 53 1.325 No Hit GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 51 1.275 No Hit GTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAACATGCACG 48 1.2 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 43 1.075 No Hit GCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATAC 33 0.8250000000000001 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 32 0.8 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 32 0.8 No Hit CTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 32 0.8 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 31 0.775 No Hit GTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAA 28 0.7000000000000001 No Hit CCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGG 25 0.625 No Hit GTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCG 25 0.625 No Hit CTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTT 25 0.625 No Hit CCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACAC 25 0.625 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 24 0.6 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 24 0.6 No Hit GCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAA 23 0.575 No Hit CCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACAT 23 0.575 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 22 0.5499999999999999 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTT 22 0.5499999999999999 No Hit CGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCT 22 0.5499999999999999 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 22 0.5499999999999999 No Hit GTCGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCC 22 0.5499999999999999 No Hit ATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAA 22 0.5499999999999999 No Hit GTGGCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTG 20 0.5 No Hit CTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCG 20 0.5 No Hit TGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTG 20 0.5 No Hit GTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCA 20 0.5 No Hit CTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGA 18 0.44999999999999996 No Hit CCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGA 18 0.44999999999999996 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 18 0.44999999999999996 No Hit CCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTT 17 0.42500000000000004 No Hit CTCTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATT 17 0.42500000000000004 No Hit CACATGAGCTACCATTGTAGAAATCCTCAAACAGGAGGACGGAATGGCTC 17 0.42500000000000004 No Hit GCCAGACATGGTGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTG 17 0.42500000000000004 No Hit ATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCC 16 0.4 No Hit GGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAG 16 0.4 No Hit GTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCT 16 0.4 No Hit CCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACAT 16 0.4 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 15 0.375 No Hit GTTACAGACGCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCA 15 0.375 No Hit CTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATG 15 0.375 No Hit CTTCTTTTCATGCTAATTTTTGGCAAACCGTTGGTCTCATCTTTTTGGGT 15 0.375 No Hit ATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCT 15 0.375 No Hit CCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCC 15 0.375 No Hit CTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCA 14 0.35000000000000003 No Hit CCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATA 14 0.35000000000000003 No Hit GCTAATTTTTGGCAAACCGTTGGTCTCATCTTTTTGGGTTCCAGCTGGGT 14 0.35000000000000003 No Hit GCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACA 14 0.35000000000000003 No Hit CTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAA 14 0.35000000000000003 No Hit GAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTAC 14 0.35000000000000003 No Hit CCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAAC 13 0.325 No Hit CTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGA 13 0.325 No Hit GTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTC 13 0.325 No Hit CTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCATCTTGTTCCATT 13 0.325 No Hit AGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 13 0.325 No Hit CTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTG 13 0.325 No Hit CGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGA 13 0.325 No Hit CTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACA 12 0.3 No Hit CCCTCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 12 0.3 No Hit ATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCT 12 0.3 No Hit GCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAACACGGCGTCT 12 0.3 No Hit GTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTG 12 0.3 No Hit ATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCTTTGG 12 0.3 No Hit GTCCTTCTTTTCATGCTAATTTTTGGCAAACCGTTGGTCTCATCTTTTTG 12 0.3 No Hit GCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCG 12 0.3 No Hit CTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATT 11 0.27499999999999997 No Hit CTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGAT 11 0.27499999999999997 No Hit CATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACG 11 0.27499999999999997 No Hit GGACGGAATGGCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCC 11 0.27499999999999997 No Hit GGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGC 11 0.27499999999999997 No Hit ATGGGATGTGGTTTGCCAGCACGGCTCAGAATTGCTCGGCAATCACCAGC 11 0.27499999999999997 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 11 0.27499999999999997 No Hit CTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTTTTCATGCCG 11 0.27499999999999997 No Hit CTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCT 11 0.27499999999999997 No Hit ATACAATCTAGTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACC 10 0.25 No Hit GCTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAG 10 0.25 No Hit CTCAGAATTGCTCGGCAATCACCAGCATTTGCTACAAAAAGCTTGTTTCT 10 0.25 No Hit CTCACAGCATTGGACAAAACTTCTTTTCCGCCAGCCGCTGCCTGTTTTGC 10 0.25 No Hit ATCTTTTTTAATCTTTTTCCTACCAAAGATTGTTTTCATTGATCTCTTTC 10 0.25 No Hit GGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCA 10 0.25 No Hit CGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCAT 10 0.25 No Hit AAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGG 10 0.25 No Hit GGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGT 10 0.25 No Hit CTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCT 10 0.25 No Hit GTGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCT 10 0.25 No Hit CCCCTCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 9 0.22499999999999998 No Hit CCTGTTATGAAAAAGAAAGCCAGTGCAAGCGCCTGCCATTGATGTTCCAA 9 0.22499999999999998 No Hit CTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAG 9 0.22499999999999998 No Hit CAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGC 9 0.22499999999999998 No Hit ATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCAACGTAC 9 0.22499999999999998 No Hit CCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAA 9 0.22499999999999998 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 9 0.22499999999999998 No Hit CGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGC 9 0.22499999999999998 No Hit CACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCG 9 0.22499999999999998 No Hit CTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAAAAAGAGAAA 9 0.22499999999999998 No Hit CGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTA 9 0.22499999999999998 No Hit GTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCTTCACGCA 8 0.2 No Hit GTTCACATTCTTCCTTTTTCATGAAAAAAAAGGGTTCATCTTAGTTTTTC 8 0.2 No Hit CTGCTGTCCAATTATATATTGCGCTAGCACACAACTGTCCAAAAGACAAT 8 0.2 No Hit GTATAAAACAGGCGTCAGTTAAAGTTACCAAAATCCAGTGAGACATCATA 8 0.2 No Hit ATGTTGAAAACCAACGGGCATCCTACAGTCTACGGAATAATCACAAATAT 8 0.2 No Hit GGCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCCC 8 0.2 No Hit CTTACTTATTCCATCAGTTTGCCAATGTCTTCTGTCCAGTTATCTGAAGT 8 0.2 No Hit GTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTT 8 0.2 No Hit TTCTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTG 8 0.2 No Hit CTCCAGTTCGCCCATCCTTTCTATGATTGCCTTTTTGCTTGTCTCCTGCA 8 0.2 No Hit AGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGC 8 0.2 No Hit GCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCAGGGGCACAA 8 0.2 No Hit CCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGACC 8 0.2 No Hit GCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCCCA 8 0.2 No Hit CCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTG 8 0.2 No Hit CGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCTTCACGC 8 0.2 No Hit GTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAA 8 0.2 No Hit GTCCAATTATATATTGCGCTAGCACACAACTGTCCAAAAGACAATCAGAA 8 0.2 No Hit GAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCCCATCCTTTCTATGA 7 0.17500000000000002 No Hit CTAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCC 7 0.17500000000000002 No Hit CTCCAAGCGCAAAAGAACCAACTCGACCCATAACTATTGAGGCCCTATAC 7 0.17500000000000002 No Hit CGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAAATA 7 0.17500000000000002 No Hit CATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATTC 7 0.17500000000000002 No Hit GGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTC 7 0.17500000000000002 No Hit GCCTGTTTTGCTGAATTTGCCGAACTTTTTTGGTTCACATATGTCTCTAA 7 0.17500000000000002 No Hit CACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATAA 7 0.17500000000000002 No Hit CGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAG 7 0.17500000000000002 No Hit ATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGG 7 0.17500000000000002 No Hit CTCTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTG 7 0.17500000000000002 No Hit AGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACA 7 0.17500000000000002 No Hit CCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTC 7 0.17500000000000002 No Hit CTCAACTATTTCTCTGACAACTTATATCTAGCAAACTCCAAACGAAAAAT 7 0.17500000000000002 No Hit AGCGAATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGT 7 0.17500000000000002 No Hit CTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGACCT 7 0.17500000000000002 No Hit ACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATA 7 0.17500000000000002 No Hit ATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCA 7 0.17500000000000002 No Hit GCTCAGAATTGCTCGGCAATCACCAGCATTTGCTACAAAAAGCTTGTTTC 7 0.17500000000000002 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATACGAAACA 7 0.17500000000000002 No Hit CCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 7 0.17500000000000002 No Hit GTTGGACTTGATAAGACACATTCCCAACTTCAAGAGTTCTCTTAATAAGC 7 0.17500000000000002 No Hit GCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCC 7 0.17500000000000002 No Hit CCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 7 0.17500000000000002 No Hit CACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCAT 7 0.17500000000000002 No Hit AGTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAA 7 0.17500000000000002 No Hit CTCTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAAAAAGAGA 7 0.17500000000000002 No Hit CAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAAAT 7 0.17500000000000002 No Hit GGTTTGCCAGCACGGCTCAGAATTGCTCGGCAATCACCAGCATTTGCTAC 6 0.15 No Hit GTTCTGCTGTCCAATTATATATTGCGCTAGCACACAACTGTCCAAAAGAC 6 0.15 No Hit CCGTATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTG 6 0.15 No Hit GCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCC 6 0.15 No Hit GGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTT 6 0.15 No Hit CTTTTCATGCTAATTTTTGGCAAACCGTTGGTCTCATCTTTTTGGGTTCC 6 0.15 No Hit CTTGCATTATTTTTTCCATGTTCTGTTGCCTGATCTAATCCTGGCAAACA 6 0.15 No Hit ATCTCCCACTTTCCTCACAGCATTGGACAAAACTTCTTTTCCGCCAGCCG 6 0.15 No Hit CCCTAGTCATGGGATGTGGTTTGCCAGCACGGCTCAGAATTGCTCGGCAA 6 0.15 No Hit CCCCCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACA 6 0.15 No Hit AGCAAGTGCTCCTCCAAGCGCAAAAGAACCAACTCGACCCATAACTATTG 6 0.15 No Hit TTTTTTTTGACCAAGCGGGAAAAGACAATCAATCCAAAGCAGTACTCAAA 6 0.15 No Hit CGATTAAGTATGGTATAAAACAGGCGTCAGTTAAAGTTACCAAAATCCAG 6 0.15 No Hit CGGCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCC 6 0.15 No Hit CCTGGCTAACATAAAATAAAAGGCACAACAGGTTGACCACAAAAAGTTAA 6 0.15 No Hit CTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCT 6 0.15 No Hit CGGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCC 6 0.15 No Hit CCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATAC 6 0.15 No Hit CTCTAAAGCTAATATGCGTGCATGAGCACACATATTTCTCCGATAATCAA 6 0.15 No Hit CTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTT 6 0.15 No Hit CCTTATAGGCAGTTTTACTAAAGTGAGAAGTGGCACTACTGACTGCATAA 6 0.15 No Hit GTGTTATTTTAGAGAAAATACAAGCTACAATATGACCTGGAAAAAGAGAC 6 0.15 No Hit CTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGT 6 0.15 No Hit GCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTT 6 0.15 No Hit TTTTTTAATCTTTTTCCTACCAAAGATTGTTTTCATTGATCTCTTTCCAA 6 0.15 No Hit CTCCCACTTTCCTCACAGCATTGGACAAAACTTCTTTTCCGCCAGCCGCT 6 0.15 No Hit GTCCTAGTGTTCATGGACCTTCCATCTCCCACTTTCCTCACAGCATTGGA 6 0.15 No Hit CCATTGTAGAAATCCTCAAACAGGAGGACGGAATGGCTCTCTCTGCAGAG 6 0.15 No Hit TCTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTC 6 0.15 No Hit CCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACAC 6 0.15 No Hit GTTAAAGTTACCAAAATCCAGTGAGACATCATAACATTTGTAGTCCAAAA 6 0.15 No Hit GCCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCA 6 0.15 No Hit GTTACAAGTCACCGACAAACGATCGTGGATACAAAAATAAGCCAGGTAAA 6 0.15 No Hit GTTCCCACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACA 5 0.125 No Hit CATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGC 5 0.125 No Hit GTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACATTCCCAACTT 5 0.125 No Hit AGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACA 5 0.125 No Hit CCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTAC 5 0.125 No Hit ACCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGG 5 0.125 No Hit CTCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACAC 5 0.125 No Hit GGCGCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCAGGGGCA 5 0.125 No Hit GGCAGAGATAGCAGTTCACATTCTTCCTTTTTCATGAAAAAAAAGGGTTC 5 0.125 No Hit CGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 5 0.125 No Hit GCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCA 5 0.125 No Hit CCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATA 5 0.125 No Hit CTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTC 5 0.125 No Hit GAGGAACTGTTAATCATGATTAGATTTTTTTTCAAAAAGGAGGGAAGCCC 5 0.125 No Hit ATCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTC 5 0.125 No Hit CCAATGTCTTCTGTCCAGTTATCTGAAGTTTTAAACCTATTATATGGTAA 5 0.125 No Hit CTAGCGAATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATGA 5 0.125 No Hit ATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGA 5 0.125 No Hit ACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTC 5 0.125 No Hit TACTGCTTACATCTAAATCTGAATATGCCTCCTTTTTGGTTAAAAATGGG 5 0.125 No Hit GCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGG 5 0.125 No Hit GCTAGCGAATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATG 5 0.125 No Hit GGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTA 5 0.125 No Hit GCCAATGTCTTCTGTCCAGTTATCTGAAGTTTTAAACCTATTATATGGTA 5 0.125 No Hit CGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCAT 5 0.125 No Hit ACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCAA 5 0.125 No Hit CACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACATTCCC 5 0.125 No Hit CGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATA 5 0.125 No Hit CATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAAC 5 0.125 No Hit CTCGTCTGGAGGTGGCGGCACGCTATTGTATGACGACGGCACGGGGGCGC 5 0.125 No Hit CTAGTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAA 5 0.125 No Hit CCCTGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 5 0.125 No Hit CCTTCTTTTCATGCTAATTTTTGGCAAACCGTTGGTCTCATCTTTTTGGG 5 0.125 No Hit GTCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTC 5 0.125 No Hit GGTCAATATCTTCAAAATCAGCACCGCCTCAAAAAAATTCCACACAGAAA 5 0.125 No Hit CGCTGGACACATAAAATAAAATAGACGCATACTGCCACCAGCAGTACAAA 5 0.125 No Hit GCCAGTGCAAGCGCCTGCCATTGATGTTCCAAAAACACTCAGTTCTGAGC 5 0.125 No Hit CACGCTCTTATAAAATCAAGCGATTTTGAGTAGACTCATATCACAATGGG 5 0.125 No Hit AGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCATCTT 5 0.125 No Hit ACACAGGTAGTTGGACTTGATAAGACACATTCCCAACTTCAAGAGTTCTC 5 0.125 No Hit CTTCAGAGCCTAAAAATCGGGAATTATCATAAACTGGAGCAGAAATCTTC 5 0.125 No Hit GCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAAT 5 0.125 No Hit GGCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAA 5 0.125 No Hit GCATGAACTCACGCTGGACACATAAAATAAAATAGACGCATACTGCCACC 5 0.125 No Hit GGTTGGACTTGATAAGACACATTCCCAACTTCAAGAGTTCTCTTAATAAG 5 0.125 No Hit GCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAAC 5 0.125 No Hit GTTCGGACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 5 0.125 No Hit GCCAGCACGGCTCAGAATTGCTCGGCAATCACCAGCATTTGCTACAAAAA 5 0.125 No Hit GCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGT 5 0.125 No Hit AAACTTGTAACAACGCTCAGTTGTACACTTGTATGCCAGCGGTTTCATCA 5 0.125 No Hit TCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGG 5 0.125 No Hit CTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCAGA 5 0.125 No Hit ACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAAAA 75 0.0014254256 13.282499 85-89 >>END_MODULE ERR5262783 read2 length is 60-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262783_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 60-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.103 37.0 37.0 37.0 37.0 37.0 2 35.9955 37.0 37.0 37.0 37.0 37.0 3 36.074 37.0 37.0 37.0 37.0 37.0 4 36.141 37.0 37.0 37.0 37.0 37.0 5 36.2745 37.0 37.0 37.0 37.0 37.0 6 36.1805 37.0 37.0 37.0 37.0 37.0 7 36.159 37.0 37.0 37.0 37.0 37.0 8 36.379 37.0 37.0 37.0 37.0 37.0 9 36.2455 37.0 37.0 37.0 37.0 37.0 10-14 36.269999999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.2618 37.0 37.0 37.0 37.0 37.0 20-24 36.216300000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.178399999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.160700000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.1269 37.0 37.0 37.0 37.0 37.0 40-44 36.067 37.0 37.0 37.0 37.0 37.0 45-49 36.0277 37.0 37.0 37.0 37.0 37.0 50-54 36.035700000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.0272 37.0 37.0 37.0 37.0 37.0 60-64 35.99189549887471 37.0 37.0 37.0 37.0 37.0 65-69 35.92893223305827 37.0 37.0 37.0 37.0 37.0 70-74 35.858914728682166 37.0 37.0 37.0 37.0 37.0 75-79 35.88862215553888 37.0 37.0 37.0 37.0 37.0 80-84 35.79231685268 37.0 37.0 37.0 37.0 37.0 85-89 35.793313610951564 37.0 37.0 37.0 37.0 37.0 90-94 35.678226008519175 37.0 37.0 37.0 37.0 37.0 95-99 35.646090560117884 37.0 37.0 37.0 37.0 37.0 100-104 35.64411136137526 37.0 37.0 37.0 37.0 37.0 105-109 35.59234761917189 37.0 37.0 37.0 37.0 37.0 110-114 35.49298160436612 37.0 37.0 37.0 37.0 37.0 115-119 35.46622834652557 37.0 37.0 37.0 37.0 37.0 120-124 35.40322228882464 37.0 37.0 37.0 34.6 37.0 125-129 35.4071346893626 37.0 37.0 37.0 34.6 37.0 130-134 35.30461113914508 37.0 37.0 37.0 32.2 37.0 135-139 35.25935753153267 37.0 37.0 37.0 29.8 37.0 140-144 35.21059970472429 37.0 37.0 37.0 27.4 37.0 145-149 35.21180394031566 37.0 37.0 37.0 27.4 37.0 150 35.321448467966576 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 2.0 23 6.0 24 2.0 25 8.0 26 14.0 27 13.0 28 17.0 29 21.0 30 26.0 31 43.0 32 70.0 33 135.0 34 266.0 35 650.0 36 2470.0 37 253.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.625 25.15 7.9750000000000005 29.25 2 29.299999999999997 23.5 31.75 15.45 3 18.575 26.1 33.550000000000004 21.775 4 24.474999999999998 29.049999999999997 24.7 21.775 5 29.799999999999997 32.65 19.525000000000002 18.025 6 22.325 37.025000000000006 22.15 18.5 7 24.7 18.6 36.7 20.0 8 19.85 23.200000000000003 28.575 28.375 9 23.275000000000002 20.25 30.025000000000002 26.450000000000003 10-14 25.105 26.784999999999997 25.509999999999998 22.6 15-19 24.645 26.174999999999997 26.625 22.555 20-24 25.235000000000003 27.105 26.025 21.634999999999998 25-29 24.884999999999998 26.035000000000004 26.825 22.255 30-34 25.224999999999998 26.875 25.855 22.045 35-39 24.355 25.555 28.265 21.825 40-44 24.42 25.264999999999997 26.985 23.330000000000002 45-49 25.419999999999998 25.82 27.08 21.68 50-54 23.369999999999997 26.77 27.625 22.235 55-59 23.69 27.465 27.884999999999998 20.96 60-64 24.3498699739948 25.495099019803963 28.350670134026807 21.804360872174435 65-69 25.1262815703926 26.291572893223307 26.70667666916729 21.875468867216803 70-74 24.53613403350838 24.836209052263065 28.327081770442607 22.30057514378595 75-79 23.335833958489623 25.68642160540135 28.64216054013503 22.335583895973993 80-84 24.4606837179038 26.407728114520246 28.354772511136694 20.77681565643926 85-89 24.193871419987982 25.710995393550974 28.63008211496095 21.4650510715001 90-94 24.570283137058382 25.246805311951892 28.288649461287896 21.89426208970183 95-99 24.61631056274451 25.754840004012436 28.4180960979035 21.210753335339554 100-104 25.280024109699127 26.289617760811694 27.615651213019238 20.814706916469937 105-109 25.663270452940584 25.638051044083525 28.518107535559366 20.180570967416525 110-114 23.974060188468943 26.694700577566117 28.00689026243794 21.324348971527005 115-119 24.522813106052872 25.798713892007758 27.605389404919872 22.073083597019497 120-124 25.426769877479877 25.43189624237453 28.574357922796946 20.566975957348642 125-129 25.13454771268888 26.335127302835854 28.234320016559717 20.296004967915547 130-134 26.046560183735256 26.020461426036125 27.972648501931307 19.960329888297316 135-139 24.91167009439435 25.89780098085746 28.302483784211358 20.888045140536835 140-144 24.51237107892909 25.549083524822315 28.546999412173356 21.39154598407524 145-149 26.101285290563553 25.06316598923432 28.930023069317805 19.90552565088432 150 23.81615598885794 24.37325905292479 31.16991643454039 20.64066852367688 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 0.5 24 0.5 25 1.0 26 1.5 27 3.0 28 2.5 29 4.5 30 8.5 31 9.5 32 9.5 33 18.5 34 20.5 35 30.5 36 66.5 37 94.5 38 129.5 39 141.5 40 133.5 41 181.0 42 251.0 43 310.0 44 402.0 45 362.5 46 210.5 47 163.5 48 155.0 49 129.0 50 113.5 51 111.5 52 111.0 53 83.5 54 50.5 55 65.5 56 84.0 57 74.5 58 67.5 59 51.5 60 34.0 61 44.0 62 45.5 63 30.0 64 17.5 65 12.0 66 18.5 67 19.0 68 26.0 69 24.5 70 10.5 71 13.0 72 13.5 73 8.0 74 6.0 75 5.5 76 6.5 77 6.0 78 3.0 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 60-61 1.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 4.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 5.0 98-99 0.0 100-101 4.0 102-103 2.0 104-105 11.0 106-107 9.0 108-109 2.0 110-111 13.0 112-113 4.0 114-115 19.0 116-117 8.0 118-119 3.0 120-121 8.0 122-123 12.0 124-125 20.0 126-127 11.0 128-129 13.0 130-131 15.0 132-133 14.0 134-135 14.0 136-137 21.0 138-139 15.0 140-141 25.0 142-143 31.0 144-145 54.0 146-147 42.0 148-149 25.0 150-151 3590.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 44.487932159165034 17.05 2 20.221787345075015 15.5 3 12.720156555772993 14.625 4 8.545335942596216 13.100000000000001 5 4.174820613176777 8.0 6 4.174820613176777 9.6 7 1.1089367253750815 2.9749999999999996 8 1.5003261578604044 4.6 9 0.5870841487279843 2.025 >10 2.4787997390737115 12.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 24 0.6 No Hit GGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTC 19 0.475 No Hit CACGTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTA 18 0.44999999999999996 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 18 0.44999999999999996 No Hit CACGAAACCATAGACTTTGACGTGCATGTTGGTCATTTTTCATATTTCGA 17 0.42500000000000004 No Hit GGATGATGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTT 16 0.4 No Hit ATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTG 16 0.4 No Hit GATAGATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTT 16 0.4 No Hit GTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGA 15 0.375 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 15 0.375 No Hit AACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGA 15 0.375 No Hit ATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCA 14 0.35000000000000003 No Hit GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 14 0.35000000000000003 No Hit ATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAAGGG 14 0.35000000000000003 No Hit GTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCA 14 0.35000000000000003 No Hit GAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAG 13 0.325 No Hit CTCAAGTTCAGCCTTTAACCGAGAGTTATTGGGAAGAGAAGTGGAGAAAT 13 0.325 No Hit GCTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAA 13 0.325 No Hit AGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTT 13 0.325 No Hit CTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCAATAAAT 13 0.325 No Hit CCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTATTGAAGTGTTGCGGG 12 0.3 No Hit GTACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCC 12 0.3 No Hit GATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGT 12 0.3 No Hit GGAAAATTCAGCGGTTCCTTAGCCAGCCTTTTCATGTTGCTGAAGTGTTC 11 0.27499999999999997 No Hit AGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGA 11 0.27499999999999997 No Hit AAATCTTTGAGACAGAAGCTTGGTTTGTCTCGGACAGAGTCGGATCCTTC 11 0.27499999999999997 No Hit AGTACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGC 11 0.27499999999999997 No Hit GTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCC 11 0.27499999999999997 No Hit CTCCCTTCTTTTGCTCTCTCTTTCCCTTTGTGCAGGCAGGCCAGTCTTGT 10 0.25 No Hit GGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAA 10 0.25 No Hit CAATGGTGTTCTCCAGGACCTGAAATCAATGTCTGCCATGTGCGCAAAAA 10 0.25 No Hit GGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTT 10 0.25 No Hit AGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATCC 10 0.25 No Hit CTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGC 10 0.25 No Hit CATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCATTGG 10 0.25 No Hit GCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGT 10 0.25 No Hit CTCCAAGTGCCACTTCTAGATCTTCTACTGAAGCATCAGGTCCTGGTACA 10 0.25 No Hit AAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGA 10 0.25 No Hit ACAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCATGAAGA 9 0.22499999999999998 No Hit GCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAAAAGG 9 0.22499999999999998 No Hit GGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGAC 9 0.22499999999999998 No Hit ATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAA 9 0.22499999999999998 No Hit GGACACTTTACTAGCCATTGAAGTCCAACCCCTAGATGATAGGAAAAGAA 9 0.22499999999999998 No Hit GTAGAATATCTGCTAAATCTCTGCTGACCAGTTCTGCTGTACCTCAACCA 9 0.22499999999999998 No Hit CGCACAACTCTCTTAGCCGCCTCCGTCCTTGCCCTAGCCCCCCGGCGCCG 9 0.22499999999999998 No Hit GTTTCGTTCTGTATACCTGGAGCTTCGAGTGAAGGGCAGGGATGCAATGC 9 0.22499999999999998 No Hit GTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAAAAAGCTGTTTGATG 9 0.22499999999999998 No Hit GTGCTGTTCAATAAATATCTATCACAGGCTTCTTGGTATGTTATAGCTAA 8 0.2 No Hit GGTGTATGGAATGTCATATGTCATGTGTACTTGAGCCTGGGGCTGTGCTG 8 0.2 No Hit GTCGGTGTGAAAAGTGATGCTCGAAATTCAGTAAGACGGTGCCTTCTGAA 8 0.2 No Hit TAAAAACCAACTGCCACCACAAATCAGCCAAACTTCGCCTATTGTGGAGG 8 0.2 No Hit ATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACT 8 0.2 No Hit GGGGTAACATTGGTTGATGGTCTTGATGGCTTGGCCGGTGGTGTTGCTGC 8 0.2 No Hit GCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACA 8 0.2 No Hit TGATATTTCAAACCTTACCATGGATCGCAAACGTAAAGTTCCTCAGTGGA 8 0.2 No Hit AAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTG 8 0.2 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCGGCTCTGACTATGACTG 8 0.2 No Hit CCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTT 8 0.2 No Hit GGCTCATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTG 8 0.2 No Hit GGTGGCCACAACTGCTGCAGTCAGGACAATCATTCCTAAACTTGGCACGG 8 0.2 No Hit CTACGCTTCAACAGATCTGATTGTTAACCTACTAAGTAGCATGCAACAGA 8 0.2 No Hit GGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAAT 8 0.2 No Hit CATCAAGTTTACACAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCTTCC 8 0.2 No Hit ATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAA 8 0.2 No Hit GGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAG 8 0.2 No Hit CATATCACGAAACCATAGACTTTGACGTGCATGTTGGTCATTTTTCATAT 8 0.2 No Hit GGATCCTTCTCCAGTGAAGGCAGTCGGTGTGAAAAGTGATGCTCGAAATT 8 0.2 No Hit GTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCAATAAATCCCCATA 8 0.2 No Hit GTGTTAGGCATGTTATCTATATTGAGGATGAACCTGTTGACGCTGACACA 8 0.2 No Hit GTCAGGACAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATA 8 0.2 No Hit GCTCCATGCCCGCAACACGTACACTGGAACGGGGATGATGTAGAGATGCT 7 0.17500000000000002 No Hit TGACGATGATGCAGCAGCAGCAGCCGATGAACAACTCATTCTACGCCTCT 7 0.17500000000000002 No Hit GCTATAGAGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGA 7 0.17500000000000002 No Hit CTTCTTGATGCCTATTACACCAATATGCGCTTTAGAAGCCTCAACGAAAA 7 0.17500000000000002 No Hit CACAATTTGAAAAAACAACATTGAAATACTTTTTTCTTAGCATGTGATAG 7 0.17500000000000002 No Hit ATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGA 7 0.17500000000000002 No Hit AGAGGGGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAAC 7 0.17500000000000002 No Hit CTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAA 7 0.17500000000000002 No Hit CCGGGACCCCAAGTTCACCAACTTTGTCGAGTTTCGCACCCACAAAGTTA 7 0.17500000000000002 No Hit GGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAG 7 0.17500000000000002 No Hit GGCCAAGAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTT 7 0.17500000000000002 No Hit GACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTC 7 0.17500000000000002 No Hit ATGGAGGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGAC 7 0.17500000000000002 No Hit GCCAAAGACAAACTAGAGCACCCAAGGTTATTGAAGTGTTGCGGGGGAGA 7 0.17500000000000002 No Hit CTCCCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGAGACTGTCATGT 7 0.17500000000000002 No Hit CGACAGGCCAAGAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCA 7 0.17500000000000002 No Hit GGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCAT 7 0.17500000000000002 No Hit CTTTGAGACAGAAGCTTGGTTTGTCTCGGACAGAGTCGGATCCTTCTCCA 6 0.15 No Hit TATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGC 6 0.15 No Hit TCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAA 6 0.15 No Hit GTTAACCAAGAAAGAAAACAAATTAGATTCTACAGTGAATCCTTGGTCTG 6 0.15 No Hit AACAGTCTGTTCATCATCAGGCCTCTTCCAATCCTTTTGCCGGCTCGTAC 6 0.15 No Hit AGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTGATA 6 0.15 No Hit ATCTATCACAGGCTTCTTGGTATGTTATAGCTAATCATTTTTTATCCAGT 6 0.15 No Hit GTGAATTTGTGCAGCTCTTCATCAAGTTTACACAGAATCTCGCACTCTTC 6 0.15 No Hit AGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCG 6 0.15 No Hit GGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTC 6 0.15 No Hit TAGTTTCCGGATCTTGTGTTCAGCTAGAGGGGCATTCATTGCAGCCATGG 6 0.15 No Hit CCTGGTTGACAGAATGCGTGCTGCTTTAGGAATGGATGCGGATAGGTACG 6 0.15 No Hit GCTCGAGGATTCCGAGAAGCACAAGGTCGAGGGCGAGGTGCACCGGCTGG 6 0.15 No Hit CATGCCCTTTGGCCTGTTGCCATCGGTGTGTATATCACTAGTTTTTGTTT 6 0.15 No Hit TCTCCAGAATTACAAGAATCTTCAAGATATTATTGCCATTTTGGGAATGG 6 0.15 No Hit CAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCA 6 0.15 No Hit GTCAGCTCCATGCCCGCAACACGTACACTGGAACGGGGATGATGTAGAGA 6 0.15 No Hit CGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGC 6 0.15 No Hit GGCTTTTCGAGAAGAATTGATTCTTCACCAGAAGTCAAAAAGGATAACCC 6 0.15 No Hit CTATATTCCATCCAACAGTGAAAAAGCTTTCATCCAGTTCTCTAAAAGGG 6 0.15 No Hit CCTGAACAGGGTGCTCTCGAAAAAACAAAGGTTATTTGAAGGTTGGCATG 6 0.15 No Hit ATCCGGTTCATCCTGCTGCAGAACCGGCAGGGGAAGACGCGCCTGGCCAA 6 0.15 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 6 0.15 No Hit GCACAAGGAAGACTTTCACCATGCCAATGCTAGAGCTGCCACCAGGTCAG 6 0.15 No Hit AGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTA 6 0.15 No Hit CATGATGGAACAACTCCTAAGGAGATTGAGAAGGGCCATTCTTTCAGCAG 6 0.15 No Hit GGATAACTGAACCAACAGTCGAATAGCATGCGTCAACTGTCCTATGCACA 6 0.15 No Hit CAATAAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTC 6 0.15 No Hit TCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTG 6 0.15 No Hit AACTAGAGCACCCAAGGTTATTGAAGTGTTGCGGGGGAGACGGTCTGATT 6 0.15 No Hit AATGGAGGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGA 6 0.15 No Hit GCTGAAGTGTTCACAGGTGCACCTGGAAAGTATGTTGAACTAAAGGAGAG 6 0.15 No Hit AGTGCCACTTCTAGATCTTCTACTGAAGCATCAGGTCCTGGTACAGGTCA 6 0.15 No Hit GAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTT 6 0.15 No Hit AATACCGCCAAGGTGCCATTGATACTCCAGAGTATCTTTCCTATGTTGAG 6 0.15 No Hit TCAGCGCCTCAGAGATCTGCAAGTTCTGCTGCTCTTTTGGTTGGAGATCC 6 0.15 No Hit GATTAATACGGCGGCCGCGTCGTCGATCTCGCTGCAAGCCTGCAAGCTAT 6 0.15 No Hit AGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTATTGAAGT 6 0.15 No Hit CTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAAAGACAA 6 0.15 No Hit TATAAGTACAGCGTCTGCACCGAATCACCGGGAATGATTAAAGCCCCGGC 6 0.15 No Hit GTGGACACTTTACTAGCCATTGAAGTCCAACCCCTAGATGATAGGAAAAG 6 0.15 No Hit GGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGA 6 0.15 No Hit CGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCG 6 0.15 No Hit ATCACTGACAATGAGTTGGCATACTTGGAATGCATCCACTTGTTTGTTGA 6 0.15 No Hit AGATGAGAAAAACGGCATGCCAAAGATTATTCCGTTTTACCCGGGAAGCA 6 0.15 No Hit AATGATTACGCATGGCAACATGGTGGCCACAACTGCTGCAGTCAGGACAA 6 0.15 No Hit AGTGACATGGCTTTTCGAGAAGAATTGATTCTTCACCAGAAGTCAAAAAG 6 0.15 No Hit AGAAAACAAATTAGATTCTACAGTGAATCCTTGGTCTGCTTATTTTGGCC 6 0.15 No Hit GAGAGAGCCTCGGGGGAAGGATCAAGAGAGGCTCGCCCGCGCGCGTGATG 6 0.15 No Hit TGAAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCA 6 0.15 No Hit CAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACT 6 0.15 No Hit CAGAGTCCTGTTGAGCTATTAAATGGTATCGCACAAGGTTCAAAAAATCT 6 0.15 No Hit GTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATT 6 0.15 No Hit CATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTC 6 0.15 No Hit CTTTGTCAGATAGTGTGGGGAGTTCTAGTGAAGTTGCTGGAAAAATGGAA 6 0.15 No Hit AAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAAAA 6 0.15 No Hit ATTCCTCGTTCCTCTACCTCCTCCTTTTGGTCTTGCACTCATGGGAAAAC 6 0.15 No Hit AGCAAGGTACATCGCTCGCCTGCTACTACGACTATTCTACTCCACTCTAC 6 0.15 No Hit TGTGCCAGCTCTGATGCTGAAGCTGATTTTGGAAATGGCAAAAAATGCAT 6 0.15 No Hit ATGAGATGCTGAGGCTGGATAACTGAACCAACAGTCGAATAGCATGCGTC 6 0.15 No Hit CTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTGATACATCA 6 0.15 No Hit GGATAGGTACGCTGCATTTAGAGAAATTGCTGGCGAATACCGCCAAGGTG 6 0.15 No Hit CACACCTGTGGTCTATGACTTCTACAACTACGACATGGAGAAGAGTGAAT 6 0.15 No Hit CTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAACAACATGCCAAAG 6 0.15 No Hit CTGGAAAGTATGTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTA 5 0.125 No Hit TTTTGAGTGGGTGCGCCATTTGGAAGATGGCTCCGTAAAATTCATAGAAG 5 0.125 No Hit GCCTCCTGCTCCTCGACGCCGCCGCCGCCGGTCGACATGGGGTTCGTGTC 5 0.125 No Hit ACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGCCGTGGCAT 5 0.125 No Hit AATAAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTCA 5 0.125 No Hit AGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTC 5 0.125 No Hit AATGCATCACCAGCACTCCAGGAAGCAAGTTCAGCTCCGGGGAGACTACC 5 0.125 No Hit GTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATCCT 5 0.125 No Hit GTCGGATCCTTCTCCAGTGAAGGCAGTCGGTGTGAAAAGTGATGCTCGAA 5 0.125 No Hit CTTTGAGCCAACTTCAAAAAAGTTCCTTATGTCTACTGAACCACCTGTTT 5 0.125 No Hit CATTGATGGAACCACAAGTTGTAATTTTGAGTGGGTGCGCCATTTGGAAG 5 0.125 No Hit GCCAAGAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTA 5 0.125 No Hit CTATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATA 5 0.125 No Hit CGCGGCTCCCCCATCCCCCCACAACCCGTGCCGCCCGGTCGCCTCGCCTC 5 0.125 No Hit GGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCCACAAC 5 0.125 No Hit GTTCTGTAAATCAACCCAACCCGATTCCTAATCTGATTTCGATATGATCC 5 0.125 No Hit GGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCA 5 0.125 No Hit TTTTTTGCAATTTGTGTGGATATCACTGACAATGAGTTGGCATACTTGGA 5 0.125 No Hit ACCAATAAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGG 5 0.125 No Hit AAGGGGTTGTTTCTTGGGAGACCTCCACAGTCCAGACACAGTTACAGAAC 5 0.125 No Hit CCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTA 5 0.125 No Hit CTGTTGGCTACTGAGGTTGACGTCTCTTGTCTCCAGAGATTATTGAATTA 5 0.125 No Hit CATCAAGCAGTGATACCGCAGTTATTATGTATACAAGTGGAAGTACAGGT 5 0.125 No Hit CTTCCGTTCTGGTCAAGCTGGCGGAGATTGCTTAGAACTAAACTTCCACA 5 0.125 No Hit GGCTAGGTTGTTACCTGATCCTTGGAAGCAGAAGGAACTTCTTGATGCCT 5 0.125 No Hit GCGAGGATGGGGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTC 5 0.125 No Hit GTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAATTTTGGA 5 0.125 No Hit CTTAGCCAGCCTTTTCATGTTGCTGAAGTGTTCACAGGTGCACCTGGAAA 5 0.125 No Hit CGTCAAAAGGAGTCTTAGTGCTAGTGTTACACAAACATATTAGTAAAGAA 5 0.125 No Hit GTTCATTCGGTGCTTATCTCCTGGCGCTCTACCTTGCTTTTATCACACCT 5 0.125 No Hit CGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGTGGTC 5 0.125 No Hit GTTGTTACCTGATCCTTGGAAGCAGAAGGAACTTCTTGATGCCTATTACA 5 0.125 No Hit CACCAAGTTTCTTGTCACTTATTCAGCGGTGCTGGGACCCTGACCCTGAA 5 0.125 No Hit CGAAAAAACAAAGGTTATTTGAAGGTTGGCATGCCCTTTGGCCTGTTGCC 5 0.125 No Hit CCCTACTAGAAGCGATGTATTTGATGGTTCTGTTGGTGCTGAAGCCCAAC 5 0.125 No Hit CAAGAATCTCATAGCACTGGGCATCAGCACCAACAAGTTCGTCGGCCCAC 5 0.125 No Hit TATTTATCCTGCTGTCGATCCTTTGGACTCCACATCCAGAATGTTGTCTC 5 0.125 No Hit AGGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCG 5 0.125 No Hit AGGAGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTA 5 0.125 No Hit GTCATCCCCATCTTTCCTATTAAGGAGGGATATCTGCGAGTATGATAGAG 5 0.125 No Hit GCAAGGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCC 5 0.125 No Hit CTTTCCTATGTTGAGCAATTTGGTATATCACATCTTGTCCCTGAAATGGC 5 0.125 No Hit GTCTATTGCTATTGACGCGAACGCGTCATCCCCATCTTTCCTATTAAGGA 5 0.125 No Hit GATCCTTGGAAGCAGAAGGAACTTCTTGATGCCTATTACACCAATATGCG 5 0.125 No Hit CGATCCTTTGGACTCCACATCCAGAATGTTGTCTCCCCACGTTCTTGGTG 5 0.125 No Hit GTTTTTGAACTAGCAGCAGAGACGGTGATGTTAGCTTCTGGTGTTGCTAT 5 0.125 No Hit GTTTACACAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCA 5 0.125 No Hit CTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAAGCGCCGACCA 5 0.125 No Hit AGTATGTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGAT 5 0.125 No Hit TGATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTAC 5 0.125 No Hit GGGACTTATGGATATTGGATGCAACCGGCTGGAGACGGTATCTCGGGATA 5 0.125 No Hit CTTTACTAGCCATTGAAGTCCAACCCCTAGATGATAGGAAAAGAAAGGGG 5 0.125 No Hit AGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTT 5 0.125 No Hit GTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGA 5 0.125 No Hit TTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCAA 5 0.125 No Hit AGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTA 5 0.125 No Hit GTTTATTCTTACTTTCTCTTCTAGGTTTACCTGATATTAGATGAGTTTAT 5 0.125 No Hit CTTCTTTTCTTCCTGGGCATGAAGAACTCCATTCCCAAGCGACAGGCCAA 5 0.125 No Hit ACAAGCCCGATGCAATCAAAGGAGATATGGACTCTATAAGACCAGAAGTG 5 0.125 No Hit AAGGCCGACACGCCGTGGCATTATAGCGAGGATGGGGTGGATCTTGTTGT 5 0.125 No Hit CAGATGTCGAGGCAGCATCTTCCCTTTCTCTCATTTCATCCTACCTGATG 5 0.125 No Hit GCGCCATTTGGAAGATGGCTCCGTAAAATTCATAGAAGGAGCAAGGCAAC 5 0.125 No Hit CGGTAACCTCAAGAATCTCATAGCACTGGGCATCAGCACCAACAAGTTCG 5 0.125 No Hit CATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra Read 1987293 spots for ERR5262783.sra Written 1987293 spots for ERR5262783.sra Read 1987290 spots for ERR5262783.sra Written 1987290 spots for ERR5262783.sra SRR ids: ['ERR5262783.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ku8u_o8c ERR5262783.sra spots: 39745803 blocks: [[1, 1987290], [1987291, 3974580], [3974581, 5961870], [5961871, 7949160], [7949161, 9936450], [9936451, 11923740], [11923741, 13911030], [13911031, 15898320], [15898321, 17885610], [17885611, 19872900], [19872901, 21860190], [21860191, 23847480], [23847481, 25834770], [25834771, 27822060], [27822061, 29809350], [29809351, 31796640], [31796641, 33783930], [33783931, 35771220], [35771221, 37758510], [37758511, 39745803]] ERR5262783 file size 13100366 ERR5262783 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262783 ERR5262783_1.fastq ERR5262783_2.fastq Input file: ERR5262783_1.fastq Paired file: ERR5262783_2.fastq trimmed: ERR5262783-trimmed-pair1.fastq, ERR5262783-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:19:36 2024 >> started Fri Dec 6 11:20:18 2024 >> done (41.957s) 39745803 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 39745803 (100.00%) read pairs available; of these: 9229 ( 0.02%) trimmed read pairs available after processing 39736574 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 0 0.00% 22 1 0.00% 23 1 0.00% 24 2 0.00% 25 2 0.00% 26 0 0.00% 27 2 0.00% 28 0 0.00% 29 4 0.00% 30 0 0.00% 31 5 0.00% 32 2 0.00% 33 6 0.00% 34 6 0.00% 35 5 0.00% 36 2 0.00% 37 3 0.00% 38 1 0.00% 39 6 0.00% 40 1 0.00% 41 0 0.00% 42 3 0.00% 43 4 0.00% 44 0 0.00% 45 5 0.00% 46 1 0.00% 47 5 0.00% 48 6 0.00% 49 367 0.00% 50 379 0.00% 51 456 0.00% 52 458 0.00% 53 475 0.00% 54 467 0.00% 55 516 0.00% 56 569 0.00% 57 635 0.00% 58 765 0.00% 59 882 0.00% 60 949 0.00% 61 1067 0.00% 62 1212 0.00% 63 1365 0.00% 64 1411 0.00% 65 1564 0.00% 66 1625 0.00% 67 1950 0.00% 68 2153 0.01% 69 2472 0.01% 70 2811 0.01% 71 3106 0.01% 72 3718 0.01% 73 4182 0.01% 74 4700 0.01% 75 4982 0.01% 76 5756 0.01% 77 6386 0.02% 78 6888 0.02% 79 7572 0.02% 80 8593 0.02% 81 9781 0.02% 82 11188 0.03% 83 12521 0.03% 84 13965 0.04% 85 15021 0.04% 86 16273 0.04% 87 17785 0.04% 88 19244 0.05% 89 20313 0.05% 90 22462 0.06% 91 24221 0.06% 92 26293 0.07% 93 28465 0.07% 94 30821 0.08% 95 33138 0.08% 96 34468 0.09% 97 36205 0.09% 98 38741 0.10% 99 39961 0.10% 100 42300 0.11% 101 44122 0.11% 102 47232 0.12% 103 50373 0.13% 104 52622 0.13% 105 54833 0.14% 106 57754 0.15% 107 60080 0.15% 108 61734 0.16% 109 64500 0.16% 110 65942 0.17% 111 68200 0.17% 112 71932 0.18% 113 74659 0.19% 114 78341 0.20% 115 79706 0.20% 116 83374 0.21% 117 85430 0.21% 118 87486 0.22% 119 88438 0.22% 120 89940 0.23% 121 94030 0.24% 122 95745 0.24% 123 98083 0.25% 124 102599 0.26% 125 104058 0.26% 126 107713 0.27% 127 108380 0.27% 128 112351 0.28% 129 112215 0.28% 130 114783 0.29% 131 116159 0.29% 132 118994 0.30% 133 121261 0.31% 134 124588 0.31% 135 128207 0.32% 136 130809 0.33% 137 132950 0.33% 138 133599 0.34% 139 135303 0.34% 140 135555 0.34% 141 138736 0.35% 142 142621 0.36% 143 143225 0.36% 144 146094 0.37% 145 147417 0.37% 146 151810 0.38% 147 299466 0.75% 148 146157 0.37% 149 146280 0.37% 150 34016246 85.58% 39745803 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=39 prefix-density=0.27 prefix-fanout=2.0 sequence=AGGCTCGGGTTG criterion=fanout-score sequence-density=0.04 sequence-density-rank=17 fanout-score=131.86 fanout-score-rank=1 prefix-density=0.59 prefix-fanout=9.3 sequence=CCTTCTTCTTCGTCTCCGGCGACGTCGTCTTGTCGGCGGCCTTGAGCTTGCGTGTGAGTGTGCGCCAGTAGTTCTTGATCTCGTTGTCGGTGCGGCCGGGGAGCCTCCGTGCGATGCGTGACCATCGGCTGCCCCACTGGGAGTGGAGCTGCAGGATGAGGCGCTCCTCGTCGGGCGTGATCCGGCCGCGCTTCAGCCCTGGGTGCAGGTAGTTCACCCACCGGAGACGGCA criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=11.34 fanout-score-rank=17 prefix-density=2.41 prefix-fanout=1.7 sequence=TCTTCTTCTTCCTCCTG criterion=fanout-score sequence-density=0.11 sequence-density-rank=12 fanout-score=121.96 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=16.6 sequence=CGCCGCCGCCGTC ERR5262783 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:21:00 Started mapping on | Dec 06 11:21:01 Finished on | Dec 06 11:24:50 Mapping speed, Million of reads per hour | 624.82 Number of input reads | 39745803 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 38684440 Uniquely mapped reads % | 97.33% Average mapped length | 292.23 Number of splices: Total | 38170685 Number of splices: Annotated (sjdb) | 35649255 Number of splices: GT/AG | 37642500 Number of splices: GC/AG | 446651 Number of splices: AT/AC | 24814 Number of splices: Non-canonical | 56720 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.39 Insertion rate per base | 0.01% Insertion average length | 2.48 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 412640 % of reads mapped to multiple loci | 1.04% Number of reads mapped to too many loci | 732 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.62% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 648723 648723 648723 N_multimapping 412640 412640 412640 N_noFeature 1320971 37668764 1657187 N_ambiguous 792569 5493 114111 UnstrandedReadsAssigned:36570900 PositiveStrandReadsAssigned:1010183 NegativeStrandReadsAssigned:36913142 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262783 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262783-trimmed-pair1.fastq ERR5262783-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 39,745,803 reads, 37,216,217 reads pseudoaligned [quant] estimated average fragment length: 274.522 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,257 rounds 52973 ERR5262783.ke.tsv 35125 ERR5262783.se.tsv 88098 total ==> ERR5262783.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 663.256 0 0 PNS24247 1044 770.478 150.676 7.31064 PNS24249 1928 1654.48 490.635 11.0859 PNS24246 1044 770.478 150.676 7.31064 PNS24248 1044 770.478 150.676 7.31064 PNS24244 1471 1197.48 220.338 6.8785 PNS24243 293 99.2186 0 0 KQK14069 1603 1329.48 81209.5 2283.48 KQK14071 474 234.555 382.941 61.0323 ==> ERR5262783.se.tsv <== BRADI_1g14170v3 82594 BRADI_1g53295v3 384 BRADI_1g59795v3 890 BRADI_1g07683v3 0 BRADI_1g00485v3 53 BRADI_1g20270v3 1200 BRADI_1g74790v3 3535 BRADI_1g09890v3 0 BRADI_1g77505v3 517 BRADI_1g48960v3 0 ERR5262783 completed mapping pipeline successfully