Starting /dee2/code/volunteer_pipeline.sh ERR5262784 current disk space = 1551223590912 free memory = 1595527840 ERR5262784 SRAfilesize 5a38ad2e2119ccdd262b6a5212a3b1b5 ERR5262784.sra ERR5262784.sra file validated ERR5262784 is paired end ERR5262784 is conventional basespace ERR5262784 read1 length is 91-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262784_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 91-150 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5245 37.0 37.0 37.0 37.0 37.0 2 36.58 37.0 37.0 37.0 37.0 37.0 3 36.7265 37.0 37.0 37.0 37.0 37.0 4 36.6055 37.0 37.0 37.0 37.0 37.0 5 36.589 37.0 37.0 37.0 37.0 37.0 6 36.6485 37.0 37.0 37.0 37.0 37.0 7 36.5215 37.0 37.0 37.0 37.0 37.0 8 36.6885 37.0 37.0 37.0 37.0 37.0 9 36.6875 37.0 37.0 37.0 37.0 37.0 10-14 36.6229 37.0 37.0 37.0 37.0 37.0 15-19 36.581999999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.43769999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.2449 37.0 37.0 37.0 37.0 37.0 30-34 36.488099999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.326899999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.316300000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.2873 37.0 37.0 37.0 37.0 37.0 50-54 36.4437 37.0 37.0 37.0 37.0 37.0 55-59 36.399100000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.2485 37.0 37.0 37.0 37.0 37.0 65-69 36.2873 37.0 37.0 37.0 37.0 37.0 70-74 36.306400000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.2796 37.0 37.0 37.0 37.0 37.0 80-84 36.2954 37.0 37.0 37.0 37.0 37.0 85-89 36.3051 37.0 37.0 37.0 37.0 37.0 90-94 36.28345314601536 37.0 37.0 37.0 37.0 37.0 95-99 36.16496742931825 37.0 37.0 37.0 37.0 37.0 100-104 35.9903756034452 37.0 37.0 37.0 37.0 37.0 105-109 36.00802765890232 37.0 37.0 37.0 37.0 37.0 110-114 36.10906199535834 37.0 37.0 37.0 37.0 37.0 115-119 35.97825162730404 37.0 37.0 37.0 37.0 37.0 120-124 35.3215080191578 37.0 37.0 37.0 32.2 37.0 125-129 35.68664929117624 37.0 37.0 37.0 37.0 37.0 130-134 35.95062308731549 37.0 37.0 37.0 37.0 37.0 135-139 35.83721130378417 37.0 37.0 37.0 37.0 37.0 140-144 35.36376480563381 37.0 37.0 37.0 32.2 37.0 145-149 35.270910722322284 37.0 37.0 37.0 34.6 37.0 150 35.62603878116344 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 5.0 27 7.0 28 16.0 29 20.0 30 27.0 31 52.0 32 73.0 33 92.0 34 164.0 35 326.0 36 2745.0 37 473.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.625 14.274999999999999 19.175 32.925 2 9.00900900900901 17.29229229229229 32.58258258258258 41.11611611611611 3 25.525 18.65 31.324999999999996 24.5 4 13.325000000000001 28.275 41.349999999999994 17.05 5 13.900000000000002 45.675 27.725 12.7 6 13.05 51.075 26.025 9.85 7 7.675 35.75 49.175000000000004 7.3999999999999995 8 10.95 30.525000000000002 28.725 29.799999999999997 9 12.2 48.275 31.025000000000002 8.5 10-14 11.65 39.434999999999995 35.925000000000004 12.989999999999998 15-19 15.86 40.365 22.564999999999998 21.21 20-24 18.310000000000002 34.32 23.990000000000002 23.380000000000003 25-29 13.900000000000002 43.3 27.134999999999998 15.665000000000001 30-34 17.845 32.92 32.295 16.939999999999998 35-39 23.355 34.42 18.055 24.169999999999998 40-44 19.655 32.68 22.869999999999997 24.795 45-49 18.215 41.07 24.16 16.555 50-54 14.124999999999998 37.325 21.745 26.805 55-59 19.28 33.06 16.475 31.185000000000002 60-64 20.405 29.23 22.009999999999998 28.355000000000004 65-69 16.31 36.57 21.12 26.0 70-74 21.615000000000002 27.625 24.38 26.38 75-79 19.939999999999998 30.65 18.38 31.03 80-84 18.61 25.485000000000003 23.515 32.39 85-89 18.695 27.975 23.544999999999998 29.785 90-94 15.773154630926184 35.167033406681334 19.18883776755351 29.87097419483897 95-99 15.149847400810525 26.016910992144894 29.494171211287334 29.339070395757243 100-104 11.647812593853239 25.958554409850837 32.786064671138256 29.607568325157672 105-109 11.750539360794741 26.054889368320705 28.40299031659224 33.791580954292314 110-114 14.97628418609345 33.80764961146433 32.72782319103845 18.488243011403775 115-119 13.835776233706953 26.484759344727898 38.99173302226505 20.687731399300098 120-124 11.649342004198884 33.688360899175585 25.920426033079007 28.74187106354652 125-129 9.781709083385062 27.01220773846472 28.905441754603768 34.30064142354645 130-134 14.032889584964762 25.330200991908118 36.15244061602715 24.484468807099972 135-139 16.474508144700657 27.993441929342076 33.68944362174741 21.84260630420986 140-144 13.326879638593095 29.681617726148218 30.138754436915132 26.85274819834355 145-149 13.057760744593486 22.961949082945523 37.70599507254311 26.27429509991788 150 28.254847645429365 19.2797783933518 34.76454293628809 17.700831024930746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 0.5 20 0.0 21 0.0 22 2.5 23 5.0 24 7.5 25 7.5 26 7.5 27 13.0 28 28.5 29 44.5 30 69.5 31 122.5 32 120.5 33 97.5 34 85.0 35 84.5 36 137.0 37 139.0 38 186.0 39 293.0 40 186.5 41 114.0 42 127.5 43 52.5 44 90.5 45 147.5 46 117.0 47 180.0 48 384.5 49 680.5 50 437.0 51 8.0 52 3.0 53 4.0 54 8.5 55 5.5 56 0.0 57 0.0 58 0.5 59 0.5 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 90-91 1.0 92-93 1.0 94-95 0.0 96-97 1.0 98-99 1.0 100-101 0.0 102-103 2.0 104-105 4.0 106-107 6.0 108-109 13.0 110-111 8.0 112-113 9.0 114-115 2.0 116-117 13.0 118-119 20.0 120-121 8.0 122-123 20.0 124-125 10.0 126-127 27.0 128-129 10.0 130-131 10.0 132-133 24.0 134-135 19.0 136-137 18.0 138-139 23.0 140-141 30.0 142-143 34.0 144-145 21.0 146-147 27.0 148-149 28.0 150-151 3610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.9 #Duplication Level Percentage of deduplicated Percentage of total 1 43.43434343434344 4.3 2 14.646464646464647 2.9000000000000004 3 8.333333333333332 2.475 4 5.05050505050505 2.0 5 3.787878787878788 1.875 6 2.525252525252525 1.5 7 2.272727272727273 1.575 8 0.7575757575757576 0.6 9 1.2626262626262625 1.125 >10 13.131313131313133 29.15 >50 3.2828282828282833 20.95 >100 1.2626262626262625 15.825 >500 0.25252525252525254 15.725 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGTAATCAAATTACAACAATCGCCAAATTATCTGGCCACGTACATGAAC 629 15.725 No Hit CTCTTTATTAATGATAAGCGTAATCAAATTACAACAATCGCCAAATTATC 148 3.6999999999999997 No Hit CCTGAATCCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCT 143 3.5749999999999997 No Hit TCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAGAT 140 3.5000000000000004 No Hit CAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAGATGA 101 2.5250000000000004 No Hit GCCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGGC 101 2.5250000000000004 No Hit TTTTTTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAA 75 1.875 No Hit GTAATCAAATTACAACAATCGCCAAATTATCTGGCCACGTACATGAACAA 75 1.875 No Hit AATCAAATTACAACAATCGCCAAATTATCTGGCCACGTACATGAACAAAC 73 1.825 No Hit CGTAATCAAATTACAACAATCGCCAAATTATCTGGCCACGTACATGAACA 73 1.825 No Hit CCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAGATG 68 1.7000000000000002 No Hit GCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAACTGATT 67 1.675 No Hit GTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAATTACAACAATCG 66 1.6500000000000001 No Hit CAACAGACAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAATTT 62 1.55 No Hit CTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAGA 62 1.55 No Hit GCTCTTTATTAATGATAAGCGTAATCAAATTACAACAATCGCCAAATTAT 57 1.425 No Hit AGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAGATGAT 55 1.375 No Hit CCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGGCT 53 1.325 No Hit CAGACAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAATTTAGC 52 1.3 No Hit CCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAACTG 50 1.25 No Hit TTTTTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAAT 49 1.225 No Hit TTTTTTTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAA 47 1.175 No Hit ATCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAATCC 45 1.125 No Hit CAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGGCTC 43 1.075 No Hit CAATCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAAT 41 1.0250000000000001 No Hit ATCAAATTACAACAATCGCCAAATTATCTGGCCACGTACATGAACAAACC 36 0.8999999999999999 No Hit CTTTATTTATAAACTTCAACAGACAACAATTTTATTCAGATACAAGAGAA 35 0.8750000000000001 No Hit CAACAATCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGC 34 0.8500000000000001 No Hit GCCTTTATTTATAAACTTCAACAGACAACAATTTTATTCAGATACAAGAG 32 0.8 No Hit CATATATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGATA 30 0.75 No Hit TTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAATTACA 29 0.7250000000000001 No Hit GTCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAATCC 29 0.7250000000000001 No Hit GCTGCCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCAT 28 0.7000000000000001 No Hit ATATATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGATAC 27 0.675 No Hit GGCCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGG 26 0.65 No Hit ATTACATATATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTG 26 0.65 No Hit CTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAACTGA 26 0.65 No Hit TTTTTTTTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCA 24 0.6 No Hit ATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTG 22 0.5499999999999999 No Hit CCCTGAATCCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGC 21 0.525 No Hit CTTCAACAGACAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAA 20 0.5 No Hit CAGAAATTGGAATTACATTACATATATATAAACTCCAGATAAAGATCTGA 20 0.5 No Hit CAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAATTTAGCTATA 19 0.475 No Hit CCTTTATTTATAAACTTCAACAGACAACAATTTTATTCAGATACAAGAGA 19 0.475 No Hit GCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAATCCA 19 0.475 No Hit AGCTAGAACTGATTGTAATCGGATGAATCATGGCTCCACTAGATTCGCGG 18 0.44999999999999996 No Hit AACAGACAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAATTTA 18 0.44999999999999996 No Hit TTTTTTTTGGAATTACATTACATATATATAAACTCCAGATAAAGATCTGA 18 0.44999999999999996 No Hit TTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAATTAC 18 0.44999999999999996 No Hit TAATCAAATTACAACAATCGCCAAATTATCTGGCCACGTACATGAACAAA 17 0.42500000000000004 No Hit ATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGAT 17 0.42500000000000004 No Hit TTTTTTTTTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATC 17 0.42500000000000004 No Hit ACTCTTTATTAATGATAAGCGTAATCAAATTACAACAATCGCCAAATTAT 16 0.4 No Hit GCTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAG 15 0.375 No Hit TGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAACTGAT 15 0.375 No Hit CAGGGAATTAATCCAGGTTCTGCTAATAGCTAGCTGCAGAGACTGTTGCA 15 0.375 No Hit CCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAATCCA 15 0.375 No Hit AATCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAATC 15 0.375 No Hit TGCCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGG 14 0.35000000000000003 No Hit GACAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAATTTAGCTA 14 0.35000000000000003 No Hit TTTTTTTTTTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAAT 14 0.35000000000000003 No Hit CAAATTACAACAATCGCCAAATTATCTGGCCACGTACATGAACAAACCGC 13 0.325 No Hit GCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGGCTCCACTAG 12 0.3 No Hit TTTTTTTTTGGAATTACATTACATATATATAAACTCCAGATAAAGATCTG 12 0.3 No Hit AGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGGCTCCACTA 12 0.3 No Hit ATTACATTACATATATATAAACTCCAGATAAAGATCTGACACAGATAGAT 11 0.27499999999999997 No Hit AGACAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAATTTAGCT 11 0.27499999999999997 No Hit TTTATTTATAAACTTCAACAGACAACAATTTTATTCAGATACAAGAGAAC 11 0.27499999999999997 No Hit AGGGAATTAATCCAGGTTCTGCTAATAGCTAGCTGCAGAGACTGTTGCAG 11 0.27499999999999997 No Hit ACAACAATCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTG 10 0.25 No Hit TCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAATCCA 10 0.25 No Hit AGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGGCTCC 9 0.22499999999999998 No Hit ATTACAACAATCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACC 9 0.22499999999999998 No Hit TGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAATTACAACAATC 9 0.22499999999999998 No Hit GGAATTACATTACATATATATAAACTCCAGATAAAGATCTGACACAGATA 9 0.22499999999999998 No Hit GAATTACATTACATATATATAAACTCCAGATAAAGATCTGACACAGATAG 9 0.22499999999999998 No Hit CTGAATCCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTA 8 0.2 No Hit TTTTTTTTTTTTTTTTTGTAAAATTTACTCTTTATTAATGATAAGCGTAA 8 0.2 No Hit CCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAGAT 8 0.2 No Hit GATTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAATTACAAC 7 0.17500000000000002 No Hit ATATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTT 7 0.17500000000000002 No Hit ATCCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAAC 7 0.17500000000000002 No Hit TTTTGGAATTACATTACATATATATAAACTCCAGATAAAGATCTGACACA 7 0.17500000000000002 No Hit TTACTCTTTATTAATGATAAGCGTAATCAAATTACAACAATCGCCAAATT 7 0.17500000000000002 No Hit TTTTTTTGGAATTACATTACATATATATAAACTCCAGATAAAGATCTGAC 7 0.17500000000000002 No Hit GGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAACTGAT 7 0.17500000000000002 No Hit CTGCCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATG 7 0.17500000000000002 No Hit CCCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAACT 7 0.17500000000000002 No Hit AGTAGCTAGAACTGATTGTAATCGGATGAATCATGGCTCCACTAGATTCG 6 0.15 No Hit CCCAAAAAAAAAAAACGAGACAGAAATTGGAATTACATTACATATATATA 6 0.15 No Hit TATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTT 6 0.15 No Hit CCTCTTTATTAATGATAAGCGTAATCAAATTACAACAATCGCCAAATTAT 6 0.15 No Hit TTTTTTTTACATTACATATATATAAACTCCAGATAAAGATCTGACACAGA 6 0.15 No Hit CCAGGTTCTGCTAATAGCTAGCTGCAGAGACTGTTGCAGAGCTGGCTGAT 6 0.15 No Hit GTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACTTTGATCCAGA 6 0.15 No Hit ATCAGGGAATTAATCCAGGTTCTGCTAATAGCTAGCTGCAGAGACTGTTG 6 0.15 No Hit ACAGACAACAATTTTATTCAGATACAAGAGAACAAGACAAGCTAATTTAG 6 0.15 No Hit AAAATTTACTCTTTATTAATGATAAGCGTAATCAAATTACAACAATCGCC 6 0.15 No Hit TTTGTAAAATTTACTCTTTATTAATGATAAGCGTAATCAAATTACAACAA 5 0.125 No Hit TTTGGAATTACATTACATATATATAAACTCCAGATAAAGATCTGACACAG 5 0.125 No Hit ACATATATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGAT 5 0.125 No Hit GAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGGCTCCACT 5 0.125 No Hit TTTTTTTTTTTGATTTGTAAAATTTACTCTTTATTAATGATAAGCGTAAT 5 0.125 No Hit GGTATGAAGACATGTAAGATGCCAATTGAGCTGTGTTATGAGAATTGCCT 5 0.125 No Hit CGCCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCATGG 5 0.125 No Hit GTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAACTGA 5 0.125 No Hit GATCGCCAAATTATCTGGCCACGTACATGAACAAACCGCACCCTGCAATC 5 0.125 No Hit TATATATAAACTCCAGATAAAGATCTGACACAGATAGATAGCTGGATACT 5 0.125 No Hit CACCAGGGTTTGTTAGCAGATAGAGCACAAGTGATTCTCTCTTGTGGTAT 5 0.125 No Hit ATTTATAAACTTCAACAGACAACAATTTTATTCAGATACAAGAGAACAAG 5 0.125 No Hit GTCCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAAC 5 0.125 No Hit GCTGTCAGAGAGCTAGAGTAGCTAGAACTGATTGTAATCGGATGAATCAT 5 0.125 No Hit CTCCTGCCAGGTGATTGCAGATGCTGCCAGAGAGCTAGAGTAGCTAGAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTGC 15 1.248554E-4 141.76251 6 AATCCTG 15 1.248554E-4 141.76251 5 CCTGAAT 15 1.248554E-4 141.76251 1 CTGAATC 15 1.248554E-4 141.76251 2 GAATCCT 15 1.248554E-4 141.76251 4 GCTCTTT 10 0.0073079313 141.76251 1 TGAATCC 15 1.248554E-4 141.76251 3 TCCTGCC 20 3.92496E-4 106.32188 7 CCTGCCA 25 9.5312245E-4 85.0575 8 GCGTAAT 135 0.0 63.005554 1 CGTAATC 140 0.0 60.755363 2 TAATCAA 145 0.0 58.660347 4 GTAATCA 145 0.0 58.660347 3 AATCAAA 155 0.0 54.87581 5 AAATTAC 160 0.0 53.16094 9 TCAAATT 160 0.0 53.16094 7 ATCAAAT 160 0.0 53.16094 6 ATCCAAG 20 0.001453182 38.57483 140-144 CTAGTCT 20 0.004225795 31.071234 140-144 ACTAGTC 35 2.0525185E-6 31.071234 140-144 >>END_MODULE ERR5262784 read2 length is 91-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262784_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 91-150 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.147 37.0 37.0 37.0 37.0 37.0 2 35.982 37.0 37.0 37.0 37.0 37.0 3 36.1125 37.0 37.0 37.0 37.0 37.0 4 36.0745 37.0 37.0 37.0 37.0 37.0 5 36.188 37.0 37.0 37.0 37.0 37.0 6 36.0865 37.0 37.0 37.0 37.0 37.0 7 36.1715 37.0 37.0 37.0 37.0 37.0 8 36.276 37.0 37.0 37.0 37.0 37.0 9 36.2005 37.0 37.0 37.0 37.0 37.0 10-14 36.2193 37.0 37.0 37.0 37.0 37.0 15-19 36.2511 37.0 37.0 37.0 37.0 37.0 20-24 36.175 37.0 37.0 37.0 37.0 37.0 25-29 36.2101 37.0 37.0 37.0 37.0 37.0 30-34 36.183499999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.2055 37.0 37.0 37.0 37.0 37.0 40-44 36.1604 37.0 37.0 37.0 37.0 37.0 45-49 36.1813 37.0 37.0 37.0 37.0 37.0 50-54 36.1729 37.0 37.0 37.0 37.0 37.0 55-59 36.0908 37.0 37.0 37.0 37.0 37.0 60-64 36.0476 37.0 37.0 37.0 37.0 37.0 65-69 36.106899999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.016200000000005 37.0 37.0 37.0 37.0 37.0 75-79 36.0259 37.0 37.0 37.0 37.0 37.0 80-84 35.944599999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.9821 37.0 37.0 37.0 37.0 37.0 90-94 35.937188821029665 37.0 37.0 37.0 37.0 37.0 95-99 35.91740982024863 37.0 37.0 37.0 37.0 37.0 100-104 35.90819517564635 37.0 37.0 37.0 37.0 37.0 105-109 35.8502875614599 37.0 37.0 37.0 37.0 37.0 110-114 35.84909664862944 37.0 37.0 37.0 37.0 37.0 115-119 35.89135522105438 37.0 37.0 37.0 37.0 37.0 120-124 35.82023168667575 37.0 37.0 37.0 37.0 37.0 125-129 35.7656044096381 37.0 37.0 37.0 37.0 37.0 130-134 35.678205356045105 37.0 37.0 37.0 37.0 37.0 135-139 35.71350926631949 37.0 37.0 37.0 37.0 37.0 140-144 35.666340898021815 37.0 37.0 37.0 37.0 37.0 145-149 35.60319430429886 37.0 37.0 37.0 37.0 37.0 150 35.61683801055849 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 1.0 14 0.0 15 3.0 16 3.0 17 1.0 18 0.0 19 0.0 20 1.0 21 5.0 22 10.0 23 8.0 24 7.0 25 11.0 26 4.0 27 8.0 28 8.0 29 15.0 30 14.0 31 31.0 32 39.0 33 76.0 34 172.0 35 463.0 36 2819.0 37 297.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.2 24.474999999999998 6.9750000000000005 24.349999999999998 2 34.5 22.0 22.325 21.175 3 23.1 24.65 30.75 21.5 4 26.05 28.525 21.025 24.4 5 28.925 30.099999999999998 17.65 23.325000000000003 6 23.825 36.5 19.675 20.0 7 26.275 18.575 32.15 23.0 8 26.200000000000003 20.75 25.074999999999996 27.975 9 26.900000000000002 23.175 25.474999999999998 24.45 10-14 27.18 26.884999999999998 21.45 24.485 15-19 28.794999999999998 23.945 23.525 23.735 20-24 27.565 25.040000000000003 21.995 25.4 25-29 28.225 24.735 22.535 24.505 30-34 28.34 24.41 24.0 23.25 35-39 26.015 24.98 23.7 25.305 40-44 27.66 24.4 22.785 25.155 45-49 28.29 24.26 21.884999999999998 25.564999999999998 50-54 27.58 26.174999999999997 23.165 23.080000000000002 55-59 27.18 25.369999999999997 22.435 25.014999999999997 60-64 28.52 26.165 22.85 22.465 65-69 27.544999999999998 24.365000000000002 24.21 23.880000000000003 70-74 27.800000000000004 24.455 24.26 23.485 75-79 29.57 24.79 24.07 21.57 80-84 26.965 26.615 24.795 21.625 85-89 27.12 25.424999999999997 24.779999999999998 22.675 90-94 26.985397079415886 25.140028005601124 25.275055011002202 22.5995199039808 95-99 27.37279231500475 25.64667033571822 24.99124430880072 21.98929304047631 100-104 27.815597156872563 25.292822104314745 24.987486234858345 21.90409450395435 105-109 27.078420550900606 25.457829511815767 24.925994681651698 22.53775525563193 110-114 26.985568674941973 26.68785952164699 26.682813603794532 19.64375819961651 115-119 26.16523811938936 25.87107572145864 25.68849216412233 22.275193995029667 120-124 27.12888524758052 25.869220134159455 25.66439653848123 21.33749807977879 125-129 26.138009517897785 26.40182081522864 25.775915580384854 21.684254086488725 130-134 27.000208899101736 25.861708794652184 26.566743263003968 20.571339043242116 135-139 26.602784689501824 25.416909312298163 25.798083540685056 22.182222457514953 140-144 27.191374663072775 24.59299191374663 26.318059299191376 21.89757412398922 145-149 26.01175113942123 24.69386634451705 27.148426775026085 22.14595574103564 150 28.03556543484301 24.61794943039733 25.64601278132815 21.700472353431508 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.0 22 0.0 23 0.0 24 0.5 25 1.5 26 2.0 27 2.0 28 1.0 29 0.0 30 0.5 31 1.0 32 1.0 33 1.0 34 4.5 35 18.0 36 32.0 37 35.5 38 77.5 39 109.0 40 104.5 41 120.5 42 151.5 43 182.0 44 230.0 45 243.0 46 218.0 47 180.0 48 113.5 49 84.0 50 82.0 51 75.5 52 56.5 53 39.5 54 63.0 55 110.5 56 147.0 57 147.0 58 117.5 59 94.0 60 104.5 61 201.5 62 235.5 63 176.0 64 137.0 65 90.0 66 52.0 67 33.5 68 20.5 69 16.0 70 20.0 71 27.5 72 23.5 73 9.5 74 2.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 90-91 1.0 92-93 1.0 94-95 0.0 96-97 1.0 98-99 1.0 100-101 0.0 102-103 2.0 104-105 4.0 106-107 6.0 108-109 13.0 110-111 8.0 112-113 9.0 114-115 2.0 116-117 13.0 118-119 20.0 120-121 8.0 122-123 20.0 124-125 10.0 126-127 27.0 128-129 11.0 130-131 10.0 132-133 25.0 134-135 21.0 136-137 18.0 138-139 25.0 140-141 34.0 142-143 34.0 144-145 22.0 146-147 27.0 148-149 28.0 150-151 3599.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.65 #Duplication Level Percentage of deduplicated Percentage of total 1 45.43230016313214 13.925 2 16.39477977161501 10.05 3 9.869494290375204 9.075 4 7.096247960848287 8.7 5 5.628058727569331 8.625 6 3.3442088091353996 6.15 7 2.365415986949429 5.075 8 1.794453507340946 4.3999999999999995 9 1.468189233278956 4.05 >10 6.606851549755302 29.95 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCAAGGACCGGTTTGGGCTCATCTACGTGGACCGGGCCACGCTCGCCA 46 1.15 No Hit CCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGACGGGCC 32 0.8 No Hit GTTTCAGTCAGTTATCATAAGACAAGAATAAGACTTTCAGTCGATCTCGT 30 0.75 No Hit AGGGATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACG 28 0.7000000000000001 No Hit GGCTCATCTACGTGGACCGGGCCACGCTCGCCAGGTACCGCAAGAAGTCC 27 0.675 No Hit CACTGATCGATGAGCTGCTGGCTGGCCACGACGGGCCCCATTCGCCACGT 26 0.65 No Hit GGTTCAAGGACCGGTTTGGGCTCATCTACGTGGACCGGGCCACGCTCGCC 24 0.6 No Hit GGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACGC 24 0.6 No Hit GTTCCACTACAAGCACCTCGAGTTCGTCTACAGGGCCATCCGGGAGGGCG 23 0.575 No Hit GCTCATCTACGTGGACCGGGCCACGCTCGCCAGGTACCGCAAGAAGTCCA 22 0.5499999999999999 No Hit CTTGGATTTAATAAATTCGAGAATCGGATTTGAATGAAGTGATGAGTGAA 22 0.5499999999999999 No Hit GGGACGGGTTCAAGGACCGGTTTGGGCTCATCTACGTGGACCGGGCCACG 19 0.475 No Hit GGATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGAC 19 0.475 No Hit ACTGGCTCGAGGGATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGG 18 0.44999999999999996 No Hit AGAAGTGATGCAGGTAGAGGAAGTCTTCTTGCTTCACTAGCAGAGGCTGC 18 0.44999999999999996 No Hit AGAAGAACAGTGATATTGTTCAGATGGCAAGTTACGCACCACTCTTTGTA 18 0.44999999999999996 No Hit CAATGACCAAACGTGGAACCCAGATGCTATTGTATTCAACTCCTGGCAAC 17 0.42500000000000004 No Hit GGACTGCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTCATCT 17 0.42500000000000004 No Hit GTCACATGGATGCAAGACTAGTAGTACCAACTAGTGTGTCGTTTCAGTCA 16 0.4 No Hit GCAAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACTGA 16 0.4 No Hit CTCATCTACGTGGACCGGGCCACGCTCGCCAGGTACCGCAAGAAGTCCAG 16 0.4 No Hit CGACAATGACCAAACGTGGAACCCAGATGCTATTGTATTCAACTCCTGGC 16 0.4 No Hit AGGAGACAAGGATGTCCACACAGCCAGCAAGTACCTCGATGTCTAAAAAC 15 0.375 No Hit CGGGGACGGGTTCAAGGACCGGTTTGGGCTCATCTACGTGGACCGGGCCA 15 0.375 No Hit GTGATATTGTTCAGATGGCAAGTTACGCACCACTCTTTGTAAACGACAAT 15 0.375 No Hit GTTCATGGACTGCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGC 15 0.375 No Hit GTGAAACTCAACTTGAGCAAATCGATGGATCCATCCATGCATGCAGATCA 15 0.375 No Hit GCCTCTTCTTCATCGGATCCCTTTTGTGCGTTCTTCTTCTTCTACATCGT 14 0.35000000000000003 No Hit AATAAATTCGAGAATCGGATTTGAATGAAGTGATGAGTGAATAAATTAAG 14 0.35000000000000003 No Hit GAGCTGCTGGCTGGCCACGACGGGCCCCATTCGCCACGTACGGCTTGGAT 14 0.35000000000000003 No Hit GGCTGCTTTCCTTACTGGATTGGAGAAGAACAGTGATATTGTTCAGATGG 14 0.35000000000000003 No Hit GACGGGTTCAAGGACCGGTTTGGGCTCATCTACGTGGACCGGGCCACGCT 14 0.35000000000000003 No Hit AGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGACGGGCCCCATT 14 0.35000000000000003 No Hit GGGATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGA 14 0.35000000000000003 No Hit AGTGAATAAATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAGCTAGTCTA 14 0.35000000000000003 No Hit AGCACCTCGAGTTCGTCTACAGGGCCATCCGGGAGGGCGTGAACGTGAAG 14 0.35000000000000003 No Hit GGTAGATTGGCAGTGTGGGTCGAGAGAGCTAGTCTACCGTGGATTGCAGG 13 0.325 No Hit GAAGAACAGTGATATTGTTCAGATGGCAAGTTACGCACCACTCTTTGTAA 13 0.325 No Hit AATAAATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAGCTAGTCTACCGT 13 0.325 No Hit GCTGGCTGGCCACGACGGGCCCCATTCGCCACGTACGGCTTGGATTTAAT 13 0.325 No Hit GCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACTGATCGATGAGCTGC 13 0.325 No Hit GCTGCTGGCTGGCCACGACGGGCCCCATTCGCCACGTACGGCTTGGATTT 13 0.325 No Hit GGGGTACTTCACGTGGACGTTCATGGACTGCTTCGAGTTCGGGGACGGGT 13 0.325 No Hit CACCACTCTTTGTAAACGACAATGACCAAACGTGGAACCCAGATGCTATT 12 0.3 No Hit GGTACATTTGATAGAACATCTCGTAGTGGGCCCAAGGCTTTTGTCAGTGA 12 0.3 No Hit ACGACAATGACCAAACGTGGAACCCAGATGCTATTGTATTCAACTCCTGG 12 0.3 No Hit GGAGAACGGGACTGACGAGGCGAACAACAGCACGATCCCGATCAAGGAAG 12 0.3 No Hit CGCAAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACTG 12 0.3 No Hit CGCCACGTACGGCTTGGATTTAATAAATTCGAGAATCGGATTTGAATGAA 12 0.3 No Hit CGATGAGCTGCTGGCTGGCCACGACGGGCCCCATTCGCCACGTACGGCTT 12 0.3 No Hit AGAACAGTGATATTGTTCAGATGGCAAGTTACGCACCACTCTTTGTAAAC 12 0.3 No Hit CGAGAATCGGATTTGAATGAAGTGATGAGTGAATAAATTAAGGTAGATTG 12 0.3 No Hit ATTTGAATGAAGTGATGAGTGAATAAATTAAGGTAGATTGGCAGTGTGGG 12 0.3 No Hit ATAAATTCGAGAATCGGATTTGAATGAAGTGATGAGTGAATAAATTAAGG 11 0.27499999999999997 No Hit GTTCAGATGGCAAGTTACGCACCACTCTTTGTAAACGACAATGACCAAAC 11 0.27499999999999997 No Hit GACTAGTAGTACCAACTAGTGTGTCGTTTCAGTCAGTTATCATAAGACAA 11 0.27499999999999997 No Hit GATGTACCCAGAGATGATGGCCGAGGAGGCGACTTCTTCCCAGACACTCG 11 0.27499999999999997 No Hit GTGAACGTGAAGGGGTACTTCACGTGGACGTTCATGGACTGCTTCGAGTT 11 0.27499999999999997 No Hit ATTGTATTCAACTCCTGGCAACAGTATGGAACTCCTAGTTACTGGATGCA 11 0.27499999999999997 No Hit GCAAGATGTACCCAGAGATGATGGCCGAGGAGGCGACTTCTTCCCAGACA 11 0.27499999999999997 No Hit GGACGGGTTCAAGGACCGGTTTGGGCTCATCTACGTGGACCGGGCCACGC 11 0.27499999999999997 No Hit GGATTTAATAAATTCGAGAATCGGATTTGAATGAAGTGATGAGTGAATAA 11 0.27499999999999997 No Hit GAAGTGATGCAGGTAGAGGAAGTCTTCTTGCTTCACTAGCAGAGGCTGCT 11 0.27499999999999997 No Hit ATTCGAGAATCGGATTTGAATGAAGTGATGAGTGAATAAATTAAGGTAGA 11 0.27499999999999997 No Hit GTGAAGGGGTACTTCACGTGGACGTTCATGGACTGCTTCGAGTTCGGGGA 11 0.27499999999999997 No Hit GATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGACG 10 0.25 No Hit GTTCAATCATGTCGTGCTGCGGCGGCAACTGCGGGTGCGGGTCCGGCTGC 10 0.25 No Hit GACCAAACGTGGAACCCAGATGCTATTGTATTCAACTCCTGGCAACAGTA 10 0.25 No Hit GATCAGGTCACATGGATGCAAGACTAGTAGTACCAACTAGTGTGTCGTTT 10 0.25 No Hit CAGGTCACATGGATGCAAGACTAGTAGTACCAACTAGTGTGTCGTTTCAG 10 0.25 No Hit ACTGCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTCATCTAC 10 0.25 No Hit CGACGGGCCCCATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGAAT 10 0.25 No Hit GCGGTTTGGAGAAGTGATGCAGGTAGAGGAAGTCTTCTTGCTTCACTAGC 10 0.25 No Hit AAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACTGATC 10 0.25 No Hit GGGCCACGCTCGCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGA 10 0.25 No Hit CCCAGATGCTATTGTATTCAACTCCTGGCAACAGTATGGAACTCCTAGTT 10 0.25 No Hit GCCCCATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGAATCGGATT 10 0.25 No Hit GGAGAAGTGATGCAGGTAGAGGAAGTCTTCTTGCTTCACTAGCAGAGGCT 10 0.25 No Hit AGAGAGGCTAAACAATGGGTAGCTTTTCCATTAGCAGTTTTGAAAGCCAA 10 0.25 No Hit AATCGATGGATCCATCCATGCATGCAGATCAGGTCACATGGATGCAAGAC 10 0.25 No Hit TGATGAGTGAATAAATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAGCTA 10 0.25 No Hit GTTTGGGCTCATCTACGTGGACCGGGCCACGCTCGCCAGGTACCGCAAGA 9 0.22499999999999998 No Hit AGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACTGATCGATG 9 0.22499999999999998 No Hit CTTGACCATCCCGCGGATCTCTACGATTTTCATGTCTACACCGATTCCAA 9 0.22499999999999998 No Hit GGAAGCGCTCAAGGACAACACCCGGATCATGTTCCACTACAAGCACCTCG 9 0.22499999999999998 No Hit ACTGCACCTGCAACCCGTGCACCTGCAAGTGAAACTCAACTTGAGCAAAT 9 0.22499999999999998 No Hit CGATGGATCCATCCATGCATGCAGATCAGGTCACATGGATGCAAGACTAG 9 0.22499999999999998 No Hit GCGTTCTTCTTCTTCTACATCGTTCAATCATGTCGTGCTGCGGCGGCAAC 9 0.22499999999999998 No Hit ATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGACGG 9 0.22499999999999998 No Hit GGCCACGACGGGCCCCATTCGCCACGTACGGCTTGGATTTAATAAATTCG 9 0.22499999999999998 No Hit CGATCAAGGAAGCGCTCAAGGACAACACCCGGATCATGTTCCACTACAAG 9 0.22499999999999998 No Hit GTCGTGCTGCGGCGGCAACTGCGGGTGCGGGTCCGGCTGCAAGTGCGGCA 9 0.22499999999999998 No Hit GAGGGAGCTGCTGCTGTACATCAAGAGGACATACAAAGACCCCGCCATCT 9 0.22499999999999998 No Hit GGAAGTCTTCTTGCTTCACTAGCAGAGGCTGCTTTCCTTACTGGATTGGA 9 0.22499999999999998 No Hit GATCGATGAGCTGCTGGCTGGCCACGACGGGCCCCATTCGCCACGTACGG 9 0.22499999999999998 No Hit GGCCACGCTCGCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGAT 9 0.22499999999999998 No Hit ATCGGATTTGAATGAAGTGATGAGTGAATAAATTAAGGTAGATTGGCAGT 9 0.22499999999999998 No Hit AGGAAGCGCTCAAGGACAACACCCGGATCATGTTCCACTACAAGCACCTC 9 0.22499999999999998 No Hit GGGTACTTCACGTGGACGTTCATGGACTGCTTCGAGTTCGGGGACGGGTT 9 0.22499999999999998 No Hit GATCAAAGCCTCTTCTTCATCGGATCCCTTTTGTGCGTTCTTCTTCTTCT 8 0.2 No Hit GAAGTCTTCTTGCTTCACTAGCAGAGGCTGCTTTCCTTACTGGATTGGAG 8 0.2 No Hit GCCACGCTCGCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGATT 8 0.2 No Hit GCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAG 8 0.2 No Hit CATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGAATCGGATTTGAA 8 0.2 No Hit ACTAGTAGTACCAACTAGTGTGTCGTTTCAGTCAGTTATCATAAGACAAG 8 0.2 No Hit GAAAAACAATTGCTTAGTGTTCTTTGGGTATATGAGTCACAAATAATACC 8 0.2 No Hit GGAACCCAGATGCTATTGTATTCAACTCCTGGCAACAGTATGGAACTCCT 8 0.2 No Hit TGGCAGTGTGGGTCGAGAGAGCTAGTCTACCGTGGATTGCAGGGTGCGGT 8 0.2 No Hit GCAAGACTAGTAGTACCAACTAGTGTGTCGTTTCAGTCAGTTATCATAAG 8 0.2 No Hit GAATAAATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAGCTAGTCTACCG 8 0.2 No Hit GGCTCTTCTAGGCCACTTGACCATCCCGCGGATCTCTACGATTTTCATGT 8 0.2 No Hit GGCTTGGATTTAATAAATTCGAGAATCGGATTTGAATGAAGTGATGAGTG 8 0.2 No Hit ACTGATCGATGAGCTGCTGGCTGGCCACGACGGGCCCCATTCGCCACGTA 8 0.2 No Hit GGATTGGAGAAGAACAGTGATATTGTTCAGATGGCAAGTTACGCACCACT 8 0.2 No Hit AGTAGTACCAACTAGTGTGTCGTTTCAGTCAGTTATCATAAGACAAGAAT 8 0.2 No Hit CCAAGACTTTGTTTAACATGAAGGGTACATTTGATAGAACATCTCGTAGT 8 0.2 No Hit TGAACGTGAAGGGGTACTTCACGTGGACGTTCATGGACTGCTTCGAGTTC 8 0.2 No Hit GGACCGGTTTGGGCTCATCTACGTGGACCGGGCCACGCTCGCCAGGTACC 8 0.2 No Hit GGATGCAAGACTAGTAGTACCAACTAGTGTGTCGTTTCAGTCAGTTATCA 8 0.2 No Hit CTGATCGATGAGCTGCTGGCTGGCCACGACGGGCCCCATTCGCCACGTAC 8 0.2 No Hit GCCCAAGGCTTTTGTCAGTGAGTATGCGGTTTGGAGAAGTGATGCAGGTA 8 0.2 No Hit CGAGTTCGTCTACAGGGCCATCCGGGAGGGCGTGAACGTGAAGGGGTACT 7 0.17500000000000002 No Hit GGGCTGAGGGAGCTGCTGCTGTACATCAAGAGGACATACAAAGACCCCGC 7 0.17500000000000002 No Hit GTACCAACTAGTGTGTCGTTTCAGTCAGTTATCATAAGACAAGAATAAGA 7 0.17500000000000002 No Hit CTGGATTGGAGAAGAACAGTGATATTGTTCAGATGGCAAGTTACGCACCA 7 0.17500000000000002 No Hit AGAGGAAGTCTTCTTGCTTCACTAGCAGAGGCTGCTTTCCTTACTGGATT 7 0.17500000000000002 No Hit CCACGCTCGCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGATTC 7 0.17500000000000002 No Hit CTCAACTTGAGCAAATCGATGGATCCATCCATGCATGCAGATCAGGTCAC 7 0.17500000000000002 No Hit GCTAAACAATGGGTAGCTTTTCCATTAGCAGTTTTGAAAGCCAAGAATGA 7 0.17500000000000002 No Hit AAGACTAGTAGTACCAACTAGTGTGTCGTTTCAGTCAGTTATCATAAGAC 7 0.17500000000000002 No Hit GCTTCGAGGCCGCCGCCGCCGGAGCCGAGAACGGAGGCTGCAAGTGCGGG 7 0.17500000000000002 No Hit CCAAGTCAGGCTTCGAGGCCGCCGCCGCCGGAGCCGAGAACGGAGGCTGC 7 0.17500000000000002 No Hit CTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTCATCTACGTGG 7 0.17500000000000002 No Hit GTCTTCTTGCTTCACTAGCAGAGGCTGCTTTCCTTACTGGATTGGAGAAG 7 0.17500000000000002 No Hit ATGGACTGCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTCAT 7 0.17500000000000002 No Hit ACCGGGCCACGCTCGCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGAG 7 0.17500000000000002 No Hit TGATAGAACATCTCGTAGTGGGCCCAAGGCTTTTGTCAGTGAGTATGCGG 7 0.17500000000000002 No Hit GGGAGCTGCTGCTGTACATCAAGAGGACATACAAAGACCCCGCCATCTAC 7 0.17500000000000002 No Hit GCTCGCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCA 7 0.17500000000000002 No Hit CTACAGGGCCATCCGGGAGGGCGTGAACGTGAAGGGGTACTTCACGTGGA 7 0.17500000000000002 No Hit ATCCATGCATGCAGATCAGGTCACATGGATGCAAGACTAGTAGTACCAAC 7 0.17500000000000002 No Hit CGGATTTGAATGAAGTGATGAGTGAATAAATTAAGGTAGATTGGCAGTGT 7 0.17500000000000002 No Hit CACGACGGGCCCCATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGA 7 0.17500000000000002 No Hit CGGGACTGACGAGGCGAACAACAGCACGATCCCGATCAAGGAAGCGCTCA 7 0.17500000000000002 No Hit GCACGATCCCGATCAAGGAAGCGCTCAAGGACAACACCCGGATCATGTTC 7 0.17500000000000002 No Hit GGGACAACTGCACCTGCAACCCGTGCACCTGCAAGTGAAACTCAACTTGA 7 0.17500000000000002 No Hit CTTTTCCATTAGCAGTTTTGAAAGCCAAGAATGAGAAAAACAATTGCTTA 7 0.17500000000000002 No Hit CATGGACTGCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTCA 7 0.17500000000000002 No Hit GGCCGAGGAGGCGACTTCTTCCCAGACACTCGTCATGGGCGTTGCGCCGC 7 0.17500000000000002 No Hit GATGGATCCATCCATGCATGCAGATCAGGTCACATGGATGCAAGACTAGT 7 0.17500000000000002 No Hit GATCCCTTTTGTGCGTTCTTCTTCTTCTACATCGTTCAATCATGTCGTGC 6 0.15 No Hit AGAACGGAGGCTGCAAGTGCGGGGACAACTGCACCTGCAACCCGTGCACC 6 0.15 No Hit GGATTTGAATGAAGTGATGAGTGAATAAATTAAGGTAGATTGGCAGTGTG 6 0.15 No Hit GACGGGCCCCATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGAATC 6 0.15 No Hit CATCGGCCCACCGGCTTTCACGCCCATCTTCTTTAACTACCCGCCGGGGC 6 0.15 No Hit GACAACACCCGGATCATGTTCCACTACAAGCACCTCGAGTTCGTCTACAG 6 0.15 No Hit CGTGAACGTGAAGGGGTACTTCACGTGGACGTTCATGGACTGCTTCGAGT 6 0.15 No Hit GTTATCATAAGACAAGAATAAGACTTTCAGTCGATCTCGTGGATCCATCT 6 0.15 No Hit AGCTTATCCAGACATCCAAATGATTTCAAACTGTGATGGCTCTTCTAGGC 6 0.15 No Hit GTGATGGCTCTTCTAGGCCACTTGACCATCCCGCGGATCTCTACGATTTT 6 0.15 No Hit CATCCATGCATGCAGATCAGGTCACATGGATGCAAGACTAGTAGTACCAA 6 0.15 No Hit GACTGCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTCATCTA 6 0.15 No Hit CTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGACGGGCCC 6 0.15 No Hit GAGAAGTGATGCAGGTAGAGGAAGTCTTCTTGCTTCACTAGCAGAGGCTG 6 0.15 No Hit GACCGGTTTGGGCTCATCTACGTGGACCGGGCCACGCTCGCCAGGTACCG 6 0.15 No Hit TGAGTGAATAAATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAGCTAGTC 6 0.15 No Hit CCCTTTTGTGCGTTCTTCTTCTTCTACATCGTTCAATCATGTCGTGCTGC 6 0.15 No Hit GCACCGACAACAACGCCAACCAGACCGGCTACCGCAACGGCGTCCCCATC 6 0.15 No Hit CCGCAAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACT 6 0.15 No Hit CTGGCTCGAGGGATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGC 6 0.15 No Hit GCGTGAACGTGAAGGGGTACTTCACGTGGACGTTCATGGACTGCTTCGAG 6 0.15 No Hit GGAGACAAGGATGTCCACACAGCCAGCAAGTACCTCGATGTCTAAAAACT 6 0.15 No Hit ATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGAATCGGATTTGAAT 6 0.15 No Hit CCTCGATGTCTAAAAACTCCAGCCAGAGAGGCTAAACAATGGGTAGCTTT 6 0.15 No Hit GCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTCATCTACGTG 6 0.15 No Hit ACGACGGGCCCCATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGAA 6 0.15 No Hit GGCTGCAAGTGCGGCAACGGCTGCGGAGGGTGCAAGATGTACCCAGAGAT 6 0.15 No Hit GTACGGCTTGGATTTAATAAATTCGAGAATCGGATTTGAATGAAGTGATG 6 0.15 No Hit GATCCATCCATGCATGCAGATCAGGTCACATGGATGCAAGACTAGTAGTA 6 0.15 No Hit ATTTGATAGAACATCTCGTAGTGGGCCCAAGGCTTTTGTCAGTGAGTATG 6 0.15 No Hit CTGCAAGTGAAACTCAACTTGAGCAAATCGATGGATCCATCCATGCATGC 6 0.15 No Hit CGGGCCCCATTCGCCACGTACGGCTTGGATTTAATAAATTCGAGAATCGG 6 0.15 No Hit GCTTTCCTTACTGGATTGGAGAAGAACAGTGATATTGTTCAGATGGCAAG 6 0.15 No Hit CCGGATCATGTTCCACTACAAGCACCTCGAGTTCGTCTACAGGGCCATCC 6 0.15 No Hit GAGGGATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGCCAC 6 0.15 No Hit AAGTGATGAGTGAATAAATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAG 6 0.15 No Hit GGGCATCATCCCTGCCCTGGAGACGTCCCATGCGCTCGCCTACCTCGAGA 6 0.15 No Hit TCATGGACTGCTTCGAGTTCGGGGACGGGTTCAAGGACCGGTTTGGGCTC 6 0.15 No Hit GTATGGAACTCCTAGTTACTGGATGCAGAAGTTTTTCCGCGAATCTAGTG 6 0.15 No Hit AGATGCTATTGTATTCAACTCCTGGCAACAGTATGGAACTCCTAGTTACT 6 0.15 No Hit CGTACGGCTTGGATTTAATAAATTCGAGAATCGGATTTGAATGAAGTGAT 6 0.15 No Hit GTCAGGCTTCGAGGCCGCCGCCGCCGGAGCCGAGAACGGAGGCTGCAAGT 5 0.125 No Hit TCTACGTGGACCGGGCCACGCTCGCCAGGTACCGCAAGAAGTCCAGCTAC 5 0.125 No Hit CGCGGATCTCTACGATTTTCATGTCTACACCGATTCCAAGACTTTGTTTA 5 0.125 No Hit CAATGGGTAGCTTTTCCATTAGCAGTTTTGAAAGCCAAGAATGAGAAAAA 5 0.125 No Hit ACAAAGACCCCGCCATCTACATCACGGAGAACGGGACTGACGAGGCGAAC 5 0.125 No Hit CAGAGAGGCTAAACAATGGGTAGCTTTTCCATTAGCAGTTTTGAAAGCCA 5 0.125 No Hit AGGTACCGCAAGAAGTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACG 5 0.125 No Hit CTCGAGGGATTCCTCAAGAGACGCCACTGATCGATGAGCTGCTGGCTGGC 5 0.125 No Hit ATACAAAGACCCCGCCATCTACATCACGGAGAACGGGACTGACGAGGCGA 5 0.125 No Hit ATCTATTGGTCCATGGGAAGAGAGGCCTGGACACTTTGGAGATGTTTGGC 5 0.125 No Hit ATCAAGGAAGCGCTCAAGGACAACACCCGGATCATGTTCCACTACAAGCA 5 0.125 No Hit CAGTGAGTATGCGGTTTGGAGAAGTGATGCAGGTAGAGGAAGTCTTCTTG 5 0.125 No Hit GACCGGGCCACGCTCGCCAGGTACCGCAAGAAGTCCAGCTACTGGCTCGA 5 0.125 No Hit CAAAGCCTCTTCTTCATCGGATCCCTTTTGTGCGTTCTTCTTCTTCTACA 5 0.125 No Hit AAGTGATGCAGGTAGAGGAAGTCTTCTTGCTTCACTAGCAGAGGCTGCTT 5 0.125 No Hit CTTCTTCTTCTACATCGTTCAATCATGTCGTGCTGCGGCGGCAACTGCGG 5 0.125 No Hit GTTCGTCTACAGGGCCATCCGGGAGGGCGTGAACGTGAAGGGGTACTTCA 5 0.125 No Hit AGAGGCTAAACAATGGGTAGCTTTTCCATTAGCAGTTTTGAAAGCCAAGA 5 0.125 No Hit ACGATTTTCATGTCTACACCGATTCCAAGACTTTGTTTAACATGAAGGGT 5 0.125 No Hit TCCATGCATGCAGATCAGGTCACATGGATGCAAGACTAGTAGTACCAACT 5 0.125 No Hit TGCAAGTGAAACTCAACTTGAGCAAATCGATGGATCCATCCATGCATGCA 5 0.125 No Hit GTGGAACCCAGATGCTATTGTATTCAACTCCTGGCAACAGTATGGAACTC 5 0.125 No Hit TCGATGGATCCATCCATGCATGCAGATCAGGTCACATGGATGCAAGACTA 5 0.125 No Hit ATGCAGATCAGGTCACATGGATGCAAGACTAGTAGTACCAACTAGTGTGT 5 0.125 No Hit GTCTACAGGGCCATCCGGGAGGGCGTGAACGTGAAGGGGTACTTCACGTG 5 0.125 No Hit CAGGCTTCGAGGCCGCCGCCGCCGGAGCCGAGAACGGAGGCTGCAAGTGC 5 0.125 No Hit CTAGTGTGTCGTTTCAGTCAGTTATCATAAGACAAGAATAAGACTTTCAG 5 0.125 No Hit AGGCGAACAACAGCACGATCCCGATCAAGGAAGCGCTCAAGGACAACACC 5 0.125 No Hit CTCGGCTGGAGGGCATCATCCCTGCCCTGGAGACGTCCCATGCGCTCGCC 5 0.125 No Hit GACGTATCAATATATTTTGTGGCTAGCTGCTGTTGCTCTGCTGATTGTCG 5 0.125 No Hit CGTTCAATCATGTCGTGCTGCGGCGGCAACTGCGGGTGCGGGTCCGGCTG 5 0.125 No Hit CACACAGCCAGCAAGTACCTCGATGTCTAAAAACTCCAGCCAGAGAGGCT 5 0.125 No Hit CAGTGATATTGTTCAGATGGCAAGTTACGCACCACTCTTTGTAAACGACA 5 0.125 No Hit CGATGTCTAAAAACTCCAGCCAGAGAGGCTAAACAATGGGTAGCTTTTCC 5 0.125 No Hit GGCCATCCGGGAGGGCGTGAACGTGAAGGGGTACTTCACGTGGACGTTCA 5 0.125 No Hit AGTGTGTCGTTTCAGTCAGTTATCATAAGACAAGAATAAGACTTTCAGTC 5 0.125 No Hit ATGCAAGACTAGTAGTACCAACTAGTGTGTCGTTTCAGTCAGTTATCATA 5 0.125 No Hit GATTGGCAGTGTGGGTCGAGAGAGCTAGTCTACCGTGGATTGCAGGGTGC 5 0.125 No Hit GGGCCATCCGGGAGGGCGTGAACGTGAAGGGGTACTTCACGTGGACGTTC 5 0.125 No Hit AATGATTTCAAACTGTGATGGCTCTTCTAGGCCACTTGACCATCCCGCGG 5 0.125 No Hit AATGACCAAACGTGGAACCCAGATGCTATTGTATTCAACTCCTGGCAACA 5 0.125 No Hit CAGCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACTGATCGATGAGCT 5 0.125 No Hit AGATCAGGTCACATGGATGCAAGACTAGTAGTACCAACTAGTGTGTCGTT 5 0.125 No Hit AGTACCTCGATGTCTAAAAACTCCAGCCAGAGAGGCTAAACAATGGGTAG 5 0.125 No Hit GGTTCATGTCTAGGTGCAAGATGTACCCAGAGATGATGGCCGAGGAGGCG 5 0.125 No Hit AGACGCCACTGATCGATGAGCTGCTGGCTGGCCACGACGGGCCCCATTCG 5 0.125 No Hit GGGCGTGAACGTGAAGGGGTACTTCACGTGGACGTTCATGGACTGCTTCG 5 0.125 No Hit ACTCTTTGTAAACGACAATGACCAAACGTGGAACCCAGATGCTATTGTAT 5 0.125 No Hit ATTGGACGTGCTGAATACGACAGTGTGACAGACCAGGAGGCATTGGACGC 5 0.125 No Hit AAATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAGCTAGTCTACCGTGGA 5 0.125 No Hit CAAATCGATGGATCCATCCATGCATGCAGATCAGGTCACATGGATGCAAG 5 0.125 No Hit AATTAAGGTAGATTGGCAGTGTGGGTCGAGAGAGCTAGTCTACCGTGGAT 5 0.125 No Hit GTCCAGCTACTGGCTCGAGGGATTCCTCAAGAGACGCCACTGATCGATGA 5 0.125 No Hit AAGCCTCTTCTTCATCGGATCCCTTTTGTGCGTTCTTCTTCTTCTACATC 5 0.125 No Hit CTCGAGTTCGTCTACAGGGCCATCCGGGAGGGCGTGAACGTGAAGGGGTA 5 0.125 No Hit AGAACATCTCGTAGTGGGCCCAAGGCTTTTGTCAGTGAGTATGCGGTTTG 5 0.125 No Hit AAAGATTCTGAACAAGTTCTGTATATTCATGATAATGAGCTTATAAAGGG 5 0.125 No Hit AGGACAACACCCGGATCATGTTCCACTACAAGCACCTCGAGTTCGTCTAC 5 0.125 No Hit CTTGGACCTGACATGCGACGGCACGGGCACTCACCATAGCTACAGCATAG 5 0.125 No Hit AGGACCGGTTTGGGCTCATCTACGTGGACCGGGCCACGCTCGCCAGGTAC 5 0.125 No Hit CTTCTTGCTTCACTAGCAGAGGCTGCTTTCCTTACTGGATTGGAGAAGAA 5 0.125 No Hit GCCGCCGCCGCCGGAGCCGAGAACGGAGGCTGCAAGTGCGGGGACAACTG 5 0.125 No Hit CGTGGAACCCAGATGCTATTGTATTCAACTCCTGGCAACAGTATGGAACT 5 0.125 No Hit AGGAAGTCTTCTTGCTTCACTAGCAGAGGCTGCTTTCCTTACTGGATTGG 5 0.125 No Hit CCGGCTGCAAGTGCGGCAACGGCTGCGGAGGGTGCAAGATGTACCCAGAG 5 0.125 No Hit GCAACCCGTGCACCTGCAAGTGAAACTCAACTTGAGCAAATCGATGGATC 5 0.125 No Hit ATGAAGGGTACATTTGATAGAACATCTCGTAGTGGGCCCAAGGCTTTTGT 5 0.125 No Hit GACGAGGCGAACAACAGCACGATCCCGATCAAGGAAGCGCTCAAGGACAA 5 0.125 No Hit CATACAAAGACCCCGCCATCTACATCACGGAGAACGGGACTGACGAGGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGATGT 10 0.0073079313 141.76251 9 >>END_MODULE Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399979 spots for ERR5262784.sra Written 1399979 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra Read 1399964 spots for ERR5262784.sra Written 1399964 spots for ERR5262784.sra SRR ids: ['ERR5262784.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1tqtif91 ERR5262784.sra spots: 27999295 blocks: [[1, 1399964], [1399965, 2799928], [2799929, 4199892], [4199893, 5599856], [5599857, 6999820], [6999821, 8399784], [8399785, 9799748], [9799749, 11199712], [11199713, 12599676], [12599677, 13999640], [13999641, 15399604], [15399605, 16799568], [16799569, 18199532], [18199533, 19599496], [19599497, 20999460], [20999461, 22399424], [22399425, 23799388], [23799389, 25199352], [25199353, 26599316], [26599317, 27999295]] ERR5262784 file size 9264778 ERR5262784 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262784 ERR5262784_1.fastq ERR5262784_2.fastq Input file: ERR5262784_1.fastq Paired file: ERR5262784_2.fastq trimmed: ERR5262784-trimmed-pair1.fastq, ERR5262784-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:22:47 2024 >> started Fri Dec 6 11:23:28 2024 >> done (40.491s) 27999295 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 27999294 (100.00%) read pairs available; of these: 6445 ( 0.02%) trimmed read pairs available after processing 27992849 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 0 0.00% 21 0 0.00% 22 1 0.00% 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 2 0.00% 28 0 0.00% 29 1 0.00% 30 2 0.00% 31 2 0.00% 32 2 0.00% 33 2 0.00% 34 0 0.00% 35 5 0.00% 36 0 0.00% 37 3 0.00% 38 6 0.00% 39 3 0.00% 40 1 0.00% 41 0 0.00% 42 3 0.00% 43 1 0.00% 44 0 0.00% 45 2 0.00% 46 1 0.00% 47 0 0.00% 48 4 0.00% 49 191 0.00% 50 206 0.00% 51 262 0.00% 52 306 0.00% 53 292 0.00% 54 277 0.00% 55 303 0.00% 56 343 0.00% 57 380 0.00% 58 428 0.00% 59 471 0.00% 60 598 0.00% 61 655 0.00% 62 650 0.00% 63 737 0.00% 64 812 0.00% 65 870 0.00% 66 1001 0.00% 67 1084 0.00% 68 1120 0.00% 69 1348 0.00% 70 1497 0.01% 71 1730 0.01% 72 1967 0.01% 73 2280 0.01% 74 2400 0.01% 75 2619 0.01% 76 2911 0.01% 77 3182 0.01% 78 3516 0.01% 79 3947 0.01% 80 4324 0.02% 81 4866 0.02% 82 5793 0.02% 83 6185 0.02% 84 7025 0.03% 85 7558 0.03% 86 8190 0.03% 87 8657 0.03% 88 9417 0.03% 89 10187 0.04% 90 11138 0.04% 91 12288 0.04% 92 13211 0.05% 93 14576 0.05% 94 15723 0.06% 95 16880 0.06% 96 17672 0.06% 97 18538 0.07% 98 19566 0.07% 99 20611 0.07% 100 21737 0.08% 101 23219 0.08% 102 24447 0.09% 103 26391 0.09% 104 27594 0.10% 105 29419 0.11% 106 30591 0.11% 107 32226 0.12% 108 32373 0.12% 109 34002 0.12% 110 35109 0.13% 111 36840 0.13% 112 38371 0.14% 113 40325 0.14% 114 42379 0.15% 115 44360 0.16% 116 45174 0.16% 117 46836 0.17% 118 48138 0.17% 119 48879 0.17% 120 50256 0.18% 121 51393 0.18% 122 53058 0.19% 123 55100 0.20% 124 58427 0.21% 125 58792 0.21% 126 60670 0.22% 127 62438 0.22% 128 62772 0.22% 129 65147 0.23% 130 64985 0.23% 131 66539 0.24% 132 68412 0.24% 133 71214 0.25% 134 73315 0.26% 135 75738 0.27% 136 76338 0.27% 137 77964 0.28% 138 78613 0.28% 139 82389 0.29% 140 85172 0.30% 141 89066 0.32% 142 91753 0.33% 143 93605 0.33% 144 96587 0.34% 145 95958 0.34% 146 99257 0.35% 147 226263 0.81% 148 89759 0.32% 149 89611 0.32% 150 24649461 88.04% 27999294 reads passed initial QC criterion=sequence-density sequence-density=2.87 sequence-density-rank=1 fanout-score=2.21 fanout-score-rank=27 prefix-density=3.18 prefix-fanout=2.0 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.05 sequence-density-rank=26 fanout-score=37.43 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=2.3 sequence=CCGCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=14.03 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=29 prefix-density=13.99 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=44.60 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=1.2 sequence=GTGTGCTCATCATCTTGTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATTTCATCAGCTTGAGGTTAGAGAGATTTGGAAGATGTCTTGCAGCTGTGGATCAAGCTGCAACTGTGGCTCAAACTGCACTTGCGGGAAGATGTACCCAGACCTGGCAGAGCAGGCCAGCACCACCAGCAGCACCCAGGCCCAGGTGGTGGTTCTCGGCATGGCGCCGGAGAAGAAACAGGAGCAGTTCGAGATGGCCGGCGTGTCCGGCGAAGGGTGCAGCTGCGGCGACAACTGC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262784 ERR5262784_1.fastq ERR5262784_2.fastq Input file: ERR5262784_1.fastq Paired file: ERR5262784_2.fastq trimmed: ERR5262784-trimmed-pair1.fastq, ERR5262784-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:25:55 2024 >> started Fri Dec 6 11:26:20 2024 >> done (25.612s) 21777229 read pairs processed; of these: 193 ( 0.00%) short read pairs filtered out after trimming by size control 695 ( 0.00%) empty read pairs filtered out after trimming by size control 21776341 (100.00%) read pairs available; of these: 142 ( 0.00%) trimmed read pairs available after processing 21776199 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 1 0.00% 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 2 0.00% 28 0 0.00% 29 1 0.00% 30 1 0.00% 31 1 0.00% 32 0 0.00% 33 2 0.00% 34 0 0.00% 35 4 0.00% 36 0 0.00% 37 3 0.00% 38 5 0.00% 39 2 0.00% 40 1 0.00% 41 0 0.00% 42 2 0.00% 43 0 0.00% 44 0 0.00% 45 1 0.00% 46 1 0.00% 47 0 0.00% 48 5 0.00% 49 178 0.00% 50 190 0.00% 51 247 0.00% 52 283 0.00% 53 267 0.00% 54 261 0.00% 55 286 0.00% 56 320 0.00% 57 365 0.00% 58 409 0.00% 59 448 0.00% 60 578 0.00% 61 629 0.00% 62 173 0.00% 63 367 0.00% 64 779 0.00% 65 838 0.00% 66 950 0.00% 67 555 0.00% 68 414 0.00% 69 1287 0.01% 70 1454 0.01% 71 1540 0.01% 72 891 0.00% 73 2192 0.01% 74 1813 0.01% 75 2546 0.01% 76 1686 0.01% 77 3100 0.01% 78 2277 0.01% 79 2655 0.01% 80 3502 0.02% 81 4114 0.02% 82 4021 0.02% 83 4899 0.02% 84 6201 0.03% 85 5129 0.02% 86 6738 0.03% 87 6845 0.03% 88 6914 0.03% 89 7969 0.04% 90 8547 0.04% 91 10028 0.05% 92 10105 0.05% 93 10734 0.05% 94 12869 0.06% 95 12998 0.06% 96 13721 0.06% 97 14430 0.07% 98 14511 0.07% 99 16678 0.08% 100 16522 0.08% 101 18074 0.08% 102 19176 0.09% 103 19939 0.09% 104 21881 0.10% 105 22875 0.11% 106 23569 0.11% 107 25263 0.12% 108 25538 0.12% 109 26661 0.12% 110 26508 0.12% 111 29051 0.13% 112 30355 0.14% 113 31061 0.14% 114 33541 0.15% 115 34900 0.16% 116 34998 0.16% 117 36397 0.17% 118 37898 0.17% 119 38359 0.18% 120 39361 0.18% 121 40554 0.19% 122 41609 0.19% 123 42970 0.20% 124 45221 0.21% 125 45765 0.21% 126 47083 0.22% 127 48734 0.22% 128 48916 0.22% 129 50550 0.23% 130 50680 0.23% 131 51845 0.24% 132 53011 0.24% 133 55224 0.25% 134 57186 0.26% 135 58858 0.27% 136 58981 0.27% 137 60634 0.28% 138 61204 0.28% 139 64245 0.30% 140 66562 0.31% 141 69401 0.32% 142 71639 0.33% 143 72862 0.33% 144 74961 0.34% 145 74471 0.34% 146 77056 0.35% 147 175072 0.80% 148 69632 0.32% 149 69871 0.32% 150 19168652 88.03% criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=3.36 fanout-score-rank=35 prefix-density=0.29 prefix-fanout=2.7 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.11 sequence-density-rank=16 fanout-score=124.42 fanout-score-rank=1 prefix-density=0.71 prefix-fanout=20.0 sequence=CTGCTGCTGCTC criterion=sequence-density sequence-density=1.14 sequence-density-rank=1 fanout-score=2.11 fanout-score-rank=37 prefix-density=1.16 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=23 fanout-score=227.67 fanout-score-rank=1 prefix-density=1.05 prefix-fanout=24.0 sequence=CGCCGCCGCCGC ERR5262784 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:27:15 Started mapping on | Dec 06 11:27:15 Finished on | Dec 06 11:29:40 Mapping speed, Million of reads per hour | 695.13 Number of input reads | 27998406 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 26863770 Uniquely mapped reads % | 95.95% Average mapped length | 293.66 Number of splices: Total | 25461821 Number of splices: Annotated (sjdb) | 23797271 Number of splices: GT/AG | 25096513 Number of splices: GC/AG | 310807 Number of splices: AT/AC | 15749 Number of splices: Non-canonical | 38752 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.32 Insertion rate per base | 0.01% Insertion average length | 2.47 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 296083 % of reads mapped to multiple loci | 1.06% Number of reads mapped to too many loci | 753 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.99% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 838553 838553 838553 N_multimapping 296083 296083 296083 N_noFeature 887316 26147826 1120250 N_ambiguous 565694 3575 83313 UnstrandedReadsAssigned:25410760 PositiveStrandReadsAssigned:712369 NegativeStrandReadsAssigned:25660207 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262784 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262784-trimmed-pair1.fastq ERR5262784-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,998,406 reads, 26,038,495 reads pseudoaligned [quant] estimated average fragment length: 270.744 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,182 rounds 52973 ERR5262784.ke.tsv 35125 ERR5262784.se.tsv 88098 total ==> ERR5262784.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 666.746 0 0 PNS24247 1044 774.256 119.234 8.03074 PNS24249 1928 1658.26 394.631 12.4102 PNS24246 1044 774.256 119.234 8.03074 PNS24248 1044 774.256 119.234 8.03074 PNS24244 1471 1201.26 202.667 8.7981 PNS24243 293 96.982 0 0 KQK14069 1603 1333.26 64032 2504.51 KQK14071 474 232.659 305.575 68.4917 ==> ERR5262784.se.tsv <== BRADI_1g14170v3 64839 BRADI_1g53295v3 221 BRADI_1g59795v3 501 BRADI_1g07683v3 0 BRADI_1g00485v3 13 BRADI_1g20270v3 698 BRADI_1g74790v3 2332 BRADI_1g09890v3 0 BRADI_1g77505v3 383 BRADI_1g48960v3 0 ERR5262784 completed mapping pipeline successfully