Starting /dee2/code/volunteer_pipeline.sh ERR5262785 current disk space = 1551480999936 free memory = 1339256052 ERR5262785 SRAfilesize f199355d46cd5aa6528c49106f4421ee ERR5262785.sra ERR5262785.sra file validated ERR5262785 is paired end ERR5262785 is conventional basespace ERR5262785 read1 length is 98-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262785_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 98-150 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.598 37.0 37.0 37.0 37.0 37.0 2 36.254 37.0 37.0 37.0 37.0 37.0 3 36.583 37.0 37.0 37.0 37.0 37.0 4 36.6865 37.0 37.0 37.0 37.0 37.0 5 36.7465 37.0 37.0 37.0 37.0 37.0 6 36.72 37.0 37.0 37.0 37.0 37.0 7 36.5995 37.0 37.0 37.0 37.0 37.0 8 36.723 37.0 37.0 37.0 37.0 37.0 9 36.624 37.0 37.0 37.0 37.0 37.0 10-14 36.6813 37.0 37.0 37.0 37.0 37.0 15-19 36.6408 37.0 37.0 37.0 37.0 37.0 20-24 36.5578 37.0 37.0 37.0 37.0 37.0 25-29 36.5965 37.0 37.0 37.0 37.0 37.0 30-34 36.6254 37.0 37.0 37.0 37.0 37.0 35-39 36.5416 37.0 37.0 37.0 37.0 37.0 40-44 36.5073 37.0 37.0 37.0 37.0 37.0 45-49 36.4205 37.0 37.0 37.0 37.0 37.0 50-54 36.5056 37.0 37.0 37.0 37.0 37.0 55-59 36.5649 37.0 37.0 37.0 37.0 37.0 60-64 36.554700000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.48309999999999 37.0 37.0 37.0 37.0 37.0 70-74 36.478 37.0 37.0 37.0 37.0 37.0 75-79 36.4328 37.0 37.0 37.0 37.0 37.0 80-84 36.4531 37.0 37.0 37.0 37.0 37.0 85-89 36.456100000000006 37.0 37.0 37.0 37.0 37.0 90-94 36.4131 37.0 37.0 37.0 37.0 37.0 95-99 36.304164516129035 37.0 37.0 37.0 37.0 37.0 100-104 36.165991497874465 37.0 37.0 37.0 37.0 37.0 105-109 36.11411147627241 37.0 37.0 37.0 37.0 37.0 110-114 36.27927885121076 37.0 37.0 37.0 37.0 37.0 115-119 36.268986650343074 37.0 37.0 37.0 37.0 37.0 120-124 36.221673609840764 37.0 37.0 37.0 37.0 37.0 125-129 36.2417980171827 37.0 37.0 37.0 37.0 37.0 130-134 36.27215574921937 37.0 37.0 37.0 37.0 37.0 135-139 36.09406502726522 37.0 37.0 37.0 37.0 37.0 140-144 35.98259206442992 37.0 37.0 37.0 37.0 37.0 145-149 36.10481724590765 37.0 37.0 37.0 37.0 37.0 150 36.05961376994122 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 1.0 22 1.0 23 1.0 24 0.0 25 0.0 26 2.0 27 3.0 28 9.0 29 5.0 30 19.0 31 28.0 32 44.0 33 80.0 34 113.0 35 262.0 36 2618.0 37 812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.924999999999997 39.2 9.55 19.325 2 5.508262393590386 21.557336004006007 34.62694041061592 38.30746119178768 3 5.1 17.299999999999997 31.825 45.775 4 7.3999999999999995 46.2 25.874999999999996 20.525 5 4.65 28.15 15.7 51.5 6 8.649999999999999 53.27499999999999 24.025 14.05 7 2.225 24.15 56.875 16.75 8 5.25 26.450000000000003 16.75 51.55 9 6.4750000000000005 9.3 38.074999999999996 46.150000000000006 10-14 7.51 34.48 33.25 24.759999999999998 15-19 6.52 36.635 19.59 37.255 20-24 10.184999999999999 31.474999999999998 36.265 22.075 25-29 12.01 40.01 26.395000000000003 21.584999999999997 30-34 8.705 42.370000000000005 17.8 31.125000000000004 35-39 13.205 38.005 26.05 22.74 40-44 6.0249999999999995 34.795 33.1 26.08 45-49 7.84 22.905 47.085 22.17 50-54 8.785 40.555 22.75 27.91 55-59 12.925 43.19 27.11 16.775000000000002 60-64 3.9600000000000004 41.995 27.375 26.669999999999998 65-69 6.965000000000001 39.47 23.165 30.4 70-74 14.299999999999999 35.945 23.605 26.150000000000002 75-79 13.655000000000001 46.505 16.45 23.39 80-84 5.8500000000000005 32.9 27.855 33.395 85-89 12.015 43.815 13.44 30.73 90-94 27.505000000000003 34.975 16.535 20.985 95-99 22.611130556527826 39.011950597529875 25.011250562528126 13.36566828341417 100-104 13.923480870217556 44.836209052263065 24.711177794448613 16.52913228307077 105-109 24.593037816178313 41.617831204608066 11.409967443025295 22.37916353618833 110-114 15.321077814152606 37.84940820951901 10.284563082347017 36.544950893981365 115-119 15.913695299837926 41.17706645056726 19.87945705024311 23.0297811993517 120-124 20.736552567237162 47.94213528932355 13.477995110024448 17.84331703341483 125-129 17.28820533923152 37.77583457641068 27.478010390412017 17.457949693945785 130-134 22.840920929263465 27.049692676320454 27.64350453172205 22.465881862694033 135-139 24.624099957645065 31.70266836086404 24.407030919102073 19.26620076238882 140-144 12.150037833747703 36.87709436817641 27.083558534212514 23.889309263863368 145-149 11.088386490520149 31.10939141009342 22.193355812282352 35.608866287104085 150 16.876574307304786 40.050377833753146 22.390148334732718 20.682899524209347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.5 14 1.0 15 1.0 16 0.5 17 0.5 18 1.0 19 4.0 20 4.5 21 2.5 22 8.5 23 53.0 24 52.5 25 43.0 26 61.5 27 30.5 28 11.5 29 126.5 30 124.0 31 12.0 32 14.5 33 6.5 34 202.5 35 222.5 36 214.0 37 316.0 38 783.0 39 787.5 40 156.0 41 31.5 42 10.5 43 79.5 44 192.5 45 148.0 46 63.0 47 91.0 48 92.5 49 42.0 50 4.5 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 1.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.15 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 98-99 1.0 100-101 0.0 102-103 0.0 104-105 2.0 106-107 9.0 108-109 13.0 110-111 3.0 112-113 6.0 114-115 11.0 116-117 13.0 118-119 6.0 120-121 9.0 122-123 10.0 124-125 18.0 126-127 19.0 128-129 22.0 130-131 15.0 132-133 30.0 134-135 24.0 136-137 20.0 138-139 40.0 140-141 23.0 142-143 40.0 144-145 23.0 146-147 43.0 148-149 27.0 150-151 3573.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.025 #Duplication Level Percentage of deduplicated Percentage of total 1 42.36760124610592 3.4000000000000004 2 14.330218068535824 2.3 3 7.476635514018691 1.7999999999999998 4 6.230529595015576 2.0 5 4.672897196261682 1.875 6 3.4267912772585665 1.6500000000000001 7 2.803738317757009 1.575 8 3.115264797507788 2.0 9 1.2461059190031152 0.8999999999999999 >10 11.214953271028037 19.825 >50 0.9345794392523363 6.775 >100 1.8691588785046727 28.249999999999996 >500 0.0 0.0 >1k 0.3115264797507788 27.650000000000002 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTTA 1106 27.650000000000002 No Hit GTTTATTCTTATTACAACCACATCCACATTCCACATTCATAACTGCAAAC 400 10.0 No Hit CATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTTACAAC 188 4.7 No Hit CAACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCT 181 4.5249999999999995 No Hit GTAACGAATAATCCAAACATGGATTATTTTATTTCATTAATTGTTTATTC 132 3.3000000000000003 No Hit AACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTT 115 2.875 No Hit CACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTTACA 114 2.85 No Hit GCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACATAACGTGGTC 99 2.475 No Hit GTTTCTTATTAGGGTATCAAACGAACCAACATAACGTGGTCTCAGGCTTT 90 2.25 No Hit ATTACAACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCA 82 2.0500000000000003 No Hit GTGTAAACTCGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACA 47 1.175 No Hit AACGAATAATCCAAACATGGATTATTTTATTTCATTAATTGTTTATTCTT 46 1.15 No Hit TTTTTTTTTTAATAGTAGTAACGAATAATCCAAACATGGATTATTTTATT 45 1.125 No Hit GGGCATTCCGCTGTGTTGGAACGTCATGCACCAATCAGGCAATCCTATCA 42 1.05 No Hit CGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACATAACGTGGT 36 0.8999999999999999 No Hit GTAGTAACGAATAATCCAAACATGGATTATTTTATTTCATTAATTGTTTA 34 0.8500000000000001 No Hit ATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTTACAACA 34 0.8500000000000001 No Hit GCACCAATCAGGCAATCCTATCAAAATGTTCTATCCTAAAAGATTTCGCT 33 0.8250000000000001 No Hit GTTGGAACGTCATGCACCAATCAGGCAATCCTATCAAAATGTTCTATCCT 31 0.775 No Hit ACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTTACAA 31 0.775 No Hit CTCGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACATAACGTG 29 0.7250000000000001 No Hit GAATAATCCAAACATGGATTATTTTATTTCATTAATTGTTTATTCTTATT 25 0.625 No Hit GCAACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTC 25 0.625 No Hit TTTTTTTTTAGTAACGAATAATCCAAACATGGATTATTTTATTTCATTAA 24 0.6 No Hit GGAACGTCATGCACCAATCAGGCAATCCTATCAAAATGTTCTATCCTAAA 22 0.5499999999999999 No Hit ACAACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTC 22 0.5499999999999999 No Hit TTTTTTTTTTTAATAGTAGTAACGAATAATCCAAACATGGATTATTTTAT 19 0.475 No Hit TTTTTTTAGTAGTAACGAATAATCCAAACATGGATTATTTTATTTCATTA 18 0.44999999999999996 No Hit GACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTT 17 0.42500000000000004 No Hit GAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACTAGCCGT 16 0.4 No Hit CACCAATCAGGCAATCCTATCAAAATGTTCTATCCTAAAAGATTTCGCTA 16 0.4 No Hit GTCATGCACCAATCAGGCAATCCTATCAAAATGTTCTATCCTAAAAGATT 15 0.375 No Hit GCTAGTTTTCGCATAAATATGACAAATTTCAGAACCAAAAATACGATCCA 15 0.375 No Hit ACCAGACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTA 14 0.35000000000000003 No Hit GTAAACTCGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACATA 14 0.35000000000000003 No Hit CTGGGATTCATTATTTACTAGCCGTCCCTTAATCATCGTCCTCATCCTCC 13 0.325 No Hit TTTTTTTTTAACGAATAATCCAAACATGGATTATTTTATTTCATTAATTG 13 0.325 No Hit GCCATGATTCTGCTGCCACGCAGTTCCTAAGATCCTTCAGTAACAGCAAG 12 0.3 No Hit AGCGTTTCTTATTAGGGTATCAAACGAACCAACATAACGTGGTCTCAGGC 12 0.3 No Hit TTTTTTTTAGTAGTAACGAATAATCCAAACATGGATTATTTTATTTCATT 11 0.27499999999999997 No Hit GCATAAATATGACAAATTTCAGAACCAAAAATACGATCCACGCAACATGA 11 0.27499999999999997 No Hit CATGATTCTGCTGCCACGCAGTTCCTAAGATCCTTCAGTAACAGCAAGTG 11 0.27499999999999997 No Hit CACCAGACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTT 10 0.25 No Hit GCAAGGTTTCAGACTGAAAAGCTAGTTTTCGCATAAATATGACAAATTTC 10 0.25 No Hit TACAACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATT 10 0.25 No Hit CAGACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACT 10 0.25 No Hit GCCTGCCATGATTCTGCTGCCACGCAGTTCCTAAGATCCTTCAGTAACAG 9 0.22499999999999998 No Hit TTTTTTTTTTGACTGAAAAGCTAGTTTTCGCATAAATATGACAAATTTCA 9 0.22499999999999998 No Hit CCCATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTTACA 9 0.22499999999999998 No Hit CCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACATAACGTGGTCT 9 0.22499999999999998 No Hit TTTTTTTTAAACTCGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACC 8 0.2 No Hit AGTAACAGCAAGTGTTCTTGAAACACAACCTTGTGTTCATCACCAGACCG 8 0.2 No Hit GGACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACTA 8 0.2 No Hit TTTTTTTTTTTTAATAGTAGTAACGAATAATCCAAACATGGATTATTTTA 8 0.2 No Hit GACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACTAG 8 0.2 No Hit TTACAACCACATCCACATTCCACATTCATAACTGCAAACACTGATACCAT 8 0.2 No Hit GTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACTAGCCGTCC 8 0.2 No Hit AGACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACTA 8 0.2 No Hit CATCACCAGACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTA 8 0.2 No Hit TGTAAACTCGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACAT 8 0.2 No Hit TTTTTTTTTAATCCAAACATGGATTATTTTATTTCATTAATTGTTTATTC 7 0.17500000000000002 No Hit TTTTTTTTTTTAACGAATAATCCAAACATGGATTATTTTATTTCATTAAT 7 0.17500000000000002 No Hit TGGAACGTCATGCACCAATCAGGCAATCCTATCAAAATGTTCTATCCTAA 7 0.17500000000000002 No Hit TTTTTTTTTTTTTAATAGTAGTAACGAATAATCCAAACATGGATTATTTT 7 0.17500000000000002 No Hit ACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACTAGC 7 0.17500000000000002 No Hit GTTCATCACCAGACCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCA 7 0.17500000000000002 No Hit CAAGGTTTCAGACTGAAAAGCTAGTTTTCGCATAAATATGACAAATTTCA 7 0.17500000000000002 No Hit GTCGAGTTATATCAACTGTTAAGTTCCATGGAATGATGCTGGCATATAAT 7 0.17500000000000002 No Hit GCATTCCGCTGTGTTGGAACGTCATGCACCAATCAGGCAATCCTATCAAA 7 0.17500000000000002 No Hit TTTTTTTTTTAGTAACGAATAATCCAAACATGGATTATTTTATTTCATTA 6 0.15 No Hit TTTTTTTTTTTTTTAATAGTAGTAACGAATAATCCAAACATGGATTATTT 6 0.15 No Hit AATAGTAGTAACGAATAATCCAAACATGGATTATTTTATTTCATTAATTG 6 0.15 No Hit GCGTTTCTTATTAGGGTATCAAACGAACCAACATAACGTGGTCTCAGGCT 6 0.15 No Hit TTTTTTTTTTAATCCAAACATGGATTATTTTATTTCATTAATTGTTTATT 6 0.15 No Hit CTTCAGTAACAGCAAGTGTTCTTGAAACACAACCTTGTGTTCATCACCAG 6 0.15 No Hit CGCATAAATATGACAAATTTCAGAACCAAAAATACGATCCACGCAACATG 6 0.15 No Hit AATCAGGCAATCCTATCAAAATGTTCTATCCTAAAAGATTTCGCTACTTG 6 0.15 No Hit TGGGCATTCCGCTGTGTTGGAACGTCATGCACCAATCAGGCAATCCTATC 6 0.15 No Hit ACCTTGTGTTCATCACCAGACCGAGTAGTATAGCAGGAGTGAGCTGACTG 6 0.15 No Hit TTTTTTTTTTAACGAATAATCCAAACATGGATTATTTTATTTCATTAATT 6 0.15 No Hit GTTTCGTCGAGTTATATCAACTGTTAAGTTCCATGGAATGATGCTGGCAT 5 0.125 No Hit AAACTCGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACATAAC 5 0.125 No Hit GTGGTTAGAAGATATGGAGCATGTATCCAGCAAAACAGGCAACGTCACTT 5 0.125 No Hit CCATGATTCTGCTGCCACGCAGTTCCTAAGATCCTTCAGTAACAGCAAGT 5 0.125 No Hit CATAAATATGACAAATTTCAGAACCAAAAATACGATCCACGCAACATGAT 5 0.125 No Hit TTTTAAACTCGCCAAAAGCGTTTCTTATTAGGGTATCAAACGAACCAACA 5 0.125 No Hit CGCCTGCCATGATTCTGCTGCCACGCAGTTCCTAAGATCCTTCAGTAACA 5 0.125 No Hit GTTCCTAAGATCCTTCAGTAACAGCAAGTGTTCTTGAAACACAACCTTGT 5 0.125 No Hit TTTTTTTTTTTAGTAACGAATAATCCAAACATGGATTATTTTATTTCATT 5 0.125 No Hit AGTAACGAATAATCCAAACATGGATTATTTTATTTCATTAATTGTTTATT 5 0.125 No Hit GCCGAGTAGTATAGCAGGAGTGAGCTGACTGGGATTCATTATTTACTAGC 5 0.125 No Hit AGCCACATCCACATTCCACATTCATAACTGCAAACACTGATACCATTCTT 5 0.125 No Hit GCAGTTCCTAAGATCCTTCAGTAACAGCAAGTGTTCTTGAAACACAACCT 5 0.125 No Hit CCACGCAGTTCCTAAGATCCTTCAGTAACAGCAAGTGTTCTTGAAACACA 5 0.125 No Hit CATGCACCAATCAGGCAATCCTATCAAAATGTTCTATCCTAAAAGATTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCACAT 255 0.0 64.38309 1 CCACATC 255 0.0 64.38309 2 CACATCC 265 0.0 61.953533 3 ACATCCA 270 0.0 60.806248 4 TTATTCT 95 3.3287506E-10 60.110527 3 TTATTAC 95 3.3287506E-10 60.110527 9 GTTTATT 95 3.3287506E-10 60.110527 1 TATTCTT 95 3.3287506E-10 60.110527 4 TTTATTC 95 3.3287506E-10 60.110527 2 CATCCAC 290 0.0 56.612713 5 ATCCACA 295 0.0 55.653175 6 TAACGAA 45 0.009525129 47.5875 2 GTAACGA 45 0.009525129 47.5875 1 TTCTTAT 135 1.2732926E-10 47.587498 6 CTTATTA 135 1.2732926E-10 47.587498 8 TCTTATT 135 1.2732926E-10 47.587498 7 ACCATCA 130 0.0 31.46281 140-144 CATCAAG 30 2.3888895E-5 31.462809 140-144 CCATCAA 30 2.3888895E-5 31.462809 140-144 ATCAAGC 20 0.0039739385 31.462809 140-144 >>END_MODULE ERR5262785 read2 length is 98-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262785_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 98-150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1525 37.0 37.0 37.0 37.0 37.0 2 36.0425 37.0 37.0 37.0 37.0 37.0 3 35.931 37.0 37.0 37.0 37.0 37.0 4 35.999 37.0 37.0 37.0 37.0 37.0 5 36.2365 37.0 37.0 37.0 37.0 37.0 6 36.07 37.0 37.0 37.0 37.0 37.0 7 36.099 37.0 37.0 37.0 37.0 37.0 8 36.1815 37.0 37.0 37.0 37.0 37.0 9 36.22 37.0 37.0 37.0 37.0 37.0 10-14 36.194100000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.178200000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.061 37.0 37.0 37.0 37.0 37.0 25-29 36.083 37.0 37.0 37.0 37.0 37.0 30-34 36.029500000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.0351 37.0 37.0 37.0 37.0 37.0 40-44 36.0516 37.0 37.0 37.0 37.0 37.0 45-49 36.0661 37.0 37.0 37.0 37.0 37.0 50-54 36.005700000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.944500000000005 37.0 37.0 37.0 37.0 37.0 60-64 35.8676 37.0 37.0 37.0 37.0 37.0 65-69 35.9131 37.0 37.0 37.0 37.0 37.0 70-74 35.7977 37.0 37.0 37.0 37.0 37.0 75-79 35.8077 37.0 37.0 37.0 37.0 37.0 80-84 35.77660000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.782399999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.7533 37.0 37.0 37.0 37.0 37.0 95-99 35.696433583395844 37.0 37.0 37.0 37.0 37.0 100-104 35.71307826956739 37.0 37.0 37.0 37.0 37.0 105-109 35.59589698786847 37.0 37.0 37.0 37.0 37.0 110-114 35.64680111303867 37.0 37.0 37.0 37.0 37.0 115-119 35.65266757443849 37.0 37.0 37.0 37.0 37.0 120-124 35.58512904014765 37.0 37.0 37.0 37.0 37.0 125-129 35.57757998947615 37.0 37.0 37.0 37.0 37.0 130-134 35.42624522496264 37.0 37.0 37.0 37.0 37.0 135-139 35.471914211707414 37.0 37.0 37.0 37.0 37.0 140-144 35.44573774519835 37.0 37.0 37.0 34.6 37.0 145-149 35.49243494530438 37.0 37.0 37.0 34.6 37.0 150 35.38649789029536 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 2.0 13 3.0 14 0.0 15 5.0 16 0.0 17 2.0 18 2.0 19 4.0 20 3.0 21 7.0 22 2.0 23 14.0 24 5.0 25 7.0 26 7.0 27 15.0 28 18.0 29 20.0 30 22.0 31 32.0 32 47.0 33 83.0 34 180.0 35 572.0 36 2702.0 37 244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.425 24.5 5.35 24.725 2 31.4 21.0 27.450000000000003 20.150000000000002 3 23.625 24.725 32.6 19.05 4 30.349999999999998 26.625 20.9 22.125 5 31.8 27.025 20.025000000000002 21.15 6 27.6 35.3 16.825000000000003 20.275000000000002 7 25.224999999999998 20.0 33.625 21.15 8 26.075 18.025 25.3 30.599999999999998 9 28.825 24.349999999999998 22.6 24.224999999999998 10-14 29.23 23.674999999999997 24.955 22.14 15-19 28.765 22.015 26.365 22.855 20-24 28.59 22.900000000000002 25.405 23.105 25-29 28.1 22.14 28.52 21.240000000000002 30-34 28.365000000000002 21.21 27.26 23.165 35-39 27.939999999999998 21.63 28.075 22.355 40-44 25.745 21.945 29.310000000000002 23.0 45-49 28.694999999999997 24.22 26.695 20.39 50-54 28.46 21.09 27.839999999999996 22.61 55-59 28.660000000000004 21.805 28.375 21.16 60-64 27.91 22.765 28.439999999999998 20.885 65-69 27.560000000000002 21.61 30.099999999999998 20.73 70-74 28.325 20.96 30.159999999999997 20.555 75-79 26.715 20.57 32.58 20.135 80-84 27.97 20.525 30.930000000000003 20.575 85-89 26.265 22.16 31.935000000000002 19.64 90-94 27.584999999999997 22.125 31.3 18.990000000000002 95-99 27.226361318065905 24.596229811490574 29.721486074303716 18.455922796139806 100-104 29.03225806451613 21.815453863465866 31.137784446111528 18.014503625906478 105-109 26.421237165038818 22.724768344603056 32.26145755071375 18.592536939644376 110-114 29.644925711407705 21.183580961974315 31.54872828003022 17.62276504658776 115-119 27.1677471636953 23.76924635332253 30.419367909238247 18.643638573743925 120-124 27.480643846780765 23.67563162184189 32.43683781581092 16.406886715566422 125-129 29.453217427087086 22.071909881178954 31.510724756956947 16.964147934777017 130-134 27.32388495206336 23.082534389328888 33.451438099208005 16.14214255939975 135-139 29.412700095409733 22.129757235237996 31.50111311353758 16.956429555814694 140-144 25.939768172462358 23.848987108655617 32.22294442638934 17.98830029249269 145-149 28.263645954800374 23.005164084624354 32.58926092509301 16.141929035482256 150 30.126582278481013 24.331926863572434 29.535864978902953 16.0056258790436 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 1.0 27 1.0 28 5.5 29 5.0 30 0.0 31 0.5 32 5.0 33 6.5 34 5.5 35 6.0 36 21.5 37 98.0 38 192.5 39 168.5 40 68.0 41 44.5 42 75.5 43 173.5 44 400.5 45 452.5 46 355.5 47 275.0 48 191.5 49 161.0 50 123.0 51 91.0 52 46.0 53 21.0 54 20.5 55 13.0 56 20.5 57 26.0 58 44.5 59 41.0 60 16.0 61 31.5 62 39.0 63 41.0 64 51.5 65 46.0 66 60.5 67 78.0 68 81.5 69 83.0 70 82.5 71 77.5 72 69.0 73 48.0 74 16.0 75 5.0 76 3.0 77 1.5 78 0.5 79 0.0 80 0.5 81 1.0 82 1.0 83 0.5 84 1.0 85 1.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 98-99 1.0 100-101 0.0 102-103 0.0 104-105 2.0 106-107 9.0 108-109 13.0 110-111 3.0 112-113 6.0 114-115 11.0 116-117 13.0 118-119 6.0 120-121 9.0 122-123 10.0 124-125 18.0 126-127 19.0 128-129 22.0 130-131 16.0 132-133 32.0 134-135 25.0 136-137 21.0 138-139 41.0 140-141 26.0 142-143 41.0 144-145 31.0 146-147 43.0 148-149 27.0 150-151 3555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.9 #Duplication Level Percentage of deduplicated Percentage of total 1 47.18875502008032 11.75 2 16.967871485943775 8.450000000000001 3 9.538152610441768 7.124999999999999 4 6.827309236947792 6.800000000000001 5 3.313253012048193 4.125 6 2.3092369477911645 3.45 7 1.706827309236948 2.9749999999999996 8 2.710843373493976 5.4 9 1.104417670682731 2.475 >10 7.630522088353414 36.125 >50 0.7028112449799196 11.325000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGAT 74 1.8499999999999999 No Hit GGTAGATCATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCA 73 1.825 No Hit CATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCAC 71 1.775 No Hit CTATCACCCTGGCTTGATGGTAGATCATCGATCAGACATTTCTTGAGCTT 61 1.525 No Hit ACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAG 61 1.525 No Hit AGATCATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTG 59 1.4749999999999999 No Hit GTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGAGGA 54 1.35 No Hit GATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTA 43 1.075 No Hit GTTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTT 38 0.95 No Hit GCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTGTCATCC 37 0.9249999999999999 No Hit GCTATCACCCTGGCTTGATGGTAGATCATCGATCAGACATTTCTTGAGCT 36 0.8999999999999999 No Hit CTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAGA 35 0.8750000000000001 No Hit GTGCACCCCAAGAACTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAGGC 35 0.8750000000000001 No Hit GGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATC 35 0.8750000000000001 No Hit AGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATC 33 0.8250000000000001 No Hit GCAGAAGGCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAG 33 0.8250000000000001 No Hit GTCATCCCCGTCGTGCAGAAGGCGTCGAATTATGGTGGTATTATGATTTG 32 0.8 No Hit AGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGT 31 0.775 No Hit CGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGT 31 0.775 No Hit AGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAGACATT 30 0.75 No Hit ACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAGACA 29 0.7250000000000001 No Hit CCTGGCTTGATGGTAGATCATCGATCAGACATTTCTTGAGCTTGTGTGGT 28 0.7000000000000001 No Hit ATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAGACATTTCTTGAG 27 0.675 No Hit GCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGG 27 0.675 No Hit GGTATTATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAGTGG 25 0.625 No Hit CATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGTGTGTGAAT 25 0.625 No Hit GGCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGA 23 0.575 No Hit GTCGGGCTGCCGGCGTCGGAGCAGAAGGTTGGGTATGTGCACCCCAAGAA 23 0.575 No Hit ATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTAT 22 0.5499999999999999 No Hit CAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGTGTGT 22 0.5499999999999999 No Hit GGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAG 21 0.525 No Hit GTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTGTCATCCCCGTCGT 21 0.525 No Hit ATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAAC 20 0.5 No Hit GAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATG 20 0.5 No Hit ATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGTGTGTGAATT 20 0.5 No Hit GTGCAGAAGGCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGA 19 0.475 No Hit GGAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTGC 18 0.44999999999999996 No Hit GTTCCTTTAATCACTGTCACTGTGTGTGAATTTGCTGTCATAGTGTTGAT 18 0.44999999999999996 No Hit GCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGAG 18 0.44999999999999996 No Hit GACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCG 17 0.42500000000000004 No Hit GGAAGGTCCTGAGCAGGTCAGAGAAGATTACCGGCTCCGACATATCACTA 16 0.4 No Hit CAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATC 16 0.4 No Hit TTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTG 16 0.4 No Hit GTGTCATCCCCGTCGTGCAGAAGGCGTCGAATTATGGTGGTATTATGATT 16 0.4 No Hit CGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGAGG 16 0.4 No Hit ACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGA 16 0.4 No Hit AGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTGTCATCCCC 16 0.4 No Hit GGGAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTG 16 0.4 No Hit CAGAAGGCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGA 16 0.4 No Hit CAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAGACAT 15 0.375 No Hit CCCCGTCGTGCAGAAGGCGTCGAATTATGGTGGTATTATGATTTGGGAGC 15 0.375 No Hit GTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAGT 14 0.35000000000000003 No Hit AAGGCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAA 14 0.35000000000000003 No Hit GATCATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGT 14 0.35000000000000003 No Hit ATTATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTA 14 0.35000000000000003 No Hit TATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTATG 14 0.35000000000000003 No Hit CTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAGGCGTCGAATTATGGTG 14 0.35000000000000003 No Hit GGAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTGTCA 14 0.35000000000000003 No Hit GGCAATTTTGCCTGACCATACTCACTCTTGACATTGTCTGGTTGAAGGAT 13 0.325 No Hit ATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACT 13 0.325 No Hit CAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTGTCATCCC 13 0.325 No Hit GTCAGGTTCTACGACGACGGCGACTGCGCGGCGTTCTGGCAGAGGGAGTG 13 0.325 No Hit GAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCA 13 0.325 No Hit AGCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTG 12 0.3 No Hit CATCCCCGTCGTGCAGAAGGCGTCGAATTATGGTGGTATTATGATTTGGG 12 0.3 No Hit GTGGGAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGC 12 0.3 No Hit GCTGGATCAGCGGAAGGAAAGGCAATTTTGCCTGACCATACTCACTCTTG 12 0.3 No Hit CCGGCGTCGGAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTA 12 0.3 No Hit AACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCA 11 0.27499999999999997 No Hit GGCTGGATCAGCGGAAGGAAAGGCAATTTTGCCTGACCATACTCACTCTT 11 0.27499999999999997 No Hit GTCGGCCGGATCTTCGTCAGGTTCTACGACGACGGCGACTGCGCGGCGTT 11 0.27499999999999997 No Hit CGCAGATATATGTCGGGCTGCCGGCGTCGGAGCAGAAGGTTGGGTATGTG 11 0.27499999999999997 No Hit TGGTAGATCATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATC 11 0.27499999999999997 No Hit GTCTGGTTGAAGGATCTGCATTTTGATTCAGCTCTGGCATAACTGAGGAT 11 0.27499999999999997 No Hit GACGGCGACTGCGCGGCGTTCTGGCAGAGGGAGTGGGAGCGGTGGACGGC 10 0.25 No Hit GGCTCGTCGGCCGGATCTTCGTCAGGTTCTACGACGACGGCGACTGCGCG 10 0.25 No Hit GGATCTTCGTCAGGTTCTACGACGACGGCGACTGCGCGGCGTTCTGGCAG 10 0.25 No Hit GGAAGCTGTACGCGCACAACAAGGACTACCGGGGCCGGACGCCGGTGCAG 10 0.25 No Hit TCTTCGTCAGGTTCTACGACGACGGCGACTGCGCGGCGTTCTGGCAGAGG 10 0.25 No Hit GGGCACTGTCGTGGATTCAAGATAGTAATAAAGAGCGGCATGGCTGGATC 10 0.25 No Hit GGAGTGGGAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCG 10 0.25 No Hit GTTGATTATCTGTTGTAAGAATGGTATCAGTGTTTGCAGTTATGAATGTG 10 0.25 No Hit CCCAAGAACTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAGGCGTCGAA 10 0.25 No Hit GCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTGCCGG 9 0.22499999999999998 No Hit CTTCTGATCACATCATCACTTGCCCCCTCTTCTTCTTGGCTTCGGTTTTC 9 0.22499999999999998 No Hit GCCATAGCTACAGCTCCGGGGAATTCTACGACGAGCTGGCCAGGAAGCTG 9 0.22499999999999998 No Hit GGTATGTGCACCCCAAGAACTTGTTCTATGGTGTCATCCCCGTCGTGCAG 9 0.22499999999999998 No Hit GATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGTG 9 0.22499999999999998 No Hit GGCAGAGGGAGTGGGAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATA 9 0.22499999999999998 No Hit GGCAAGGCAAATTGCTACAGCCAATGGCCCTAATGGAAATAATAGGGATT 9 0.22499999999999998 No Hit GGGAATTCTACGACGAGCTGGCCAGGAAGCTGTACGCGCACAACAAGGAC 9 0.22499999999999998 No Hit CTACGACGAGCTGGCCAGGAAGCTGTACGCGCACAACAAGGACTACCGGG 9 0.22499999999999998 No Hit GGGTGCAGAAGGTGTTGTCCACGGGGCTCGTCGGCCGGATCTTCGTCAGG 9 0.22499999999999998 No Hit CTATGGTGTCATCCCCGTCGTGCAGAAGGCGTCGAATTATGGTGGTATTA 9 0.22499999999999998 No Hit GGCCAGGAAGCTGTACGCGCACAACAAGGACTACCGGGGCCGGACGCCGG 8 0.2 No Hit GGAAAGGCAATTTTGCCTGACCATACTCACTCTTGACATTGTCTGGTTGA 8 0.2 No Hit AGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGTGTGTG 8 0.2 No Hit CATGTATCCAGCAAAACAGGCAACGTCACTTTAGCTTGTCAGGACGAAGA 8 0.2 No Hit CGGAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTGTC 8 0.2 No Hit GAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTGCC 8 0.2 No Hit CGTGACTCCAGTTCCCACTGACAGCACCCGCAGGAAGGGTGGAAGGAGGG 8 0.2 No Hit GTTCTATGGTGTCATCCCCGTCGTGCAGAAGGCGTCGAATTATGGTGGTA 8 0.2 No Hit AGAACTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAGGCGTCGAATTAT 8 0.2 No Hit AATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAA 8 0.2 No Hit GCGCGTACCCGCCGCCGGACCGGAGGGTGCAGAAGGTGTTGTCCACGGGG 8 0.2 No Hit CAAGTCTGTAATTGAGGAAATGGTGGTGACAAATGAGATCAAGAGTATAC 8 0.2 No Hit GTTGAAGGATCTGCATTTTGATTCAGCTCTGGCATAACTGAGGATCCGTG 8 0.2 No Hit AGAAGGTGTTGTCCACGGGGCTCGTCGGCCGGATCTTCGTCAGGTTCTAC 8 0.2 No Hit GGTTCCTTTAATCACTGTCACTGTGTGTGAATTTGCTGTCATAGTGTTGA 8 0.2 No Hit GGTTAGAAGATATGGAGCATGTATCCAGCAAAACAGGCAACGTCACTTTA 8 0.2 No Hit GGCGTCGGAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATG 8 0.2 No Hit CTCACTCTTGACATTGTCTGGTTGAAGGATCTGCATTTTGATTCAGCTCT 8 0.2 No Hit CAAGAACTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAGGCGTCGAATT 8 0.2 No Hit CCTTTAATCACTGTCACTGTGTGTGAATTTGCTGTCATAGTGTTGATTAT 8 0.2 No Hit GGACTACCGGGGCCGGACGCCGGTGCAGTTCACTTTGACGCCGCGGTGCG 8 0.2 No Hit ACTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAGGCGTCGAATTATGGT 8 0.2 No Hit TGATGGTAGATCATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTA 8 0.2 No Hit AGGGCACTGTCGTGGATTCAAGATAGTAATAAAGAGCGGCATGGCTGGAT 8 0.2 No Hit ACGAGCTGGCCAGGAAGCTGTACGCGCACAACAAGGACTACCGGGGCCGG 8 0.2 No Hit GAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTGTCAT 8 0.2 No Hit GGGAGCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACC 8 0.2 No Hit GTCAGAGAAGATTACCGGCTCCGACATATCACTAAAATCACATACTCCTC 7 0.17500000000000002 No Hit ATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGTGT 7 0.17500000000000002 No Hit ATCTTCGTCAGGTTCTACGACGACGGCGACTGCGCGGCGTTCTGGCAGAG 7 0.17500000000000002 No Hit CGTCACTTTAGCTTGTCAGGACGAAGATGTGTCTTTCAGTGACCTGGAGG 7 0.17500000000000002 No Hit GCGAGATGCGCGGCGAGGAGCTCGAGCCCTCCTCGCCGGATCCCCCGTTC 7 0.17500000000000002 No Hit CGTCGGCCGGATCTTCGTCAGGTTCTACGACGACGGCGACTGCGCGGCGT 7 0.17500000000000002 No Hit ATTTGCTGTCATAGTGTTGATTATCTGTTGTAAGAATGGTATCAGTGTTT 7 0.17500000000000002 No Hit CTTTAATCACTGTCACTGTGTGTGAATTTGCTGTCATAGTGTTGATTATC 7 0.17500000000000002 No Hit GCGGAAGGAAAGGCAATTTTGCCTGACCATACTCACTCTTGACATTGTCT 7 0.17500000000000002 No Hit GCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAGACATTTCTT 7 0.17500000000000002 No Hit GTAGATCATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCAC 7 0.17500000000000002 No Hit AAGATAGCGAGTTCTTGCCCTCAAGCATCCAGTACAACAGTGAAATATTT 7 0.17500000000000002 No Hit TGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGAT 7 0.17500000000000002 No Hit GATCTTCGTCAGGTTCTACGACGACGGCGACTGCGCGGCGTTCTGGCAGA 7 0.17500000000000002 No Hit AGAAGGCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGAT 7 0.17500000000000002 No Hit GGACAGCATCGCCCACAACCTCCTCCACCGTGGCTTCGCCGGCACCACAT 7 0.17500000000000002 No Hit CGTCGGAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGT 7 0.17500000000000002 No Hit ATCACCCTGGCTTGATGGTAGATCATCGATCAGACATTTCTTGAGCTTGT 6 0.15 No Hit TCACTCTTGACATTGTCTGGTTGAAGGATCTGCATTTTGATTCAGCTCTG 6 0.15 No Hit GGATGATTCGATCATTGAGCTTGCAAATGAGGTGAGGAAGGTCCTGAGCA 6 0.15 No Hit CGGGGCTCGTCGGCCGGATCTTCGTCAGGTTCTACGACGACGGCGACTGC 6 0.15 No Hit CGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTGCCGGC 6 0.15 No Hit GGAGCATGTATCCAGCAAAACAGGCAACGTCACTTTAGCTTGTCAGGACG 6 0.15 No Hit GAGAAGATTACCGGCTCCGACATATCACTAAAATCACATACTCCTCTTGT 6 0.15 No Hit GATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTATGCTA 6 0.15 No Hit GAGCTCGAGCCCTCCTCGCCGGATCCCCCGTTCGAGCTCTTCGACGACGA 6 0.15 No Hit GAGGAGCTCGAGCCCTCCTCGCCGGATCCCCCGTTCGAGCTCTTCGACGA 6 0.15 No Hit TGTGTGGTTCCTTTAATCACTGTCACTGTGTGTGAATTTGCTGTCATAGT 6 0.15 No Hit GGTGAGGAAGGTCCTGAGCAGGTCAGAGAAGATTACCGGCTCCGACATAT 6 0.15 No Hit CCTATTTTAACCTCTGAGCCAGAAATGGTCGACAAGTCTGTAATTGAGGA 6 0.15 No Hit GCAGAGGATGATGACGACAAAGAAGTGGAGGAGTGGTTAGAAGATATGGA 6 0.15 No Hit ATGTGCACCCCAAGAACTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAG 6 0.15 No Hit ATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAG 6 0.15 No Hit GGTGTCATCCCCGTCGTGCAGAAGGCGTCGAATTATGGTGGTATTATGAT 6 0.15 No Hit TCTACGACGAGCTGGCCAGGAAGCTGTACGCGCACAACAAGGACTACCGG 6 0.15 No Hit CATCCGTTACCCTCTTGCTATGGCAACCAACAACTTGCTCCTCTGTGGGT 6 0.15 No Hit GATGATGACGACAAAGAAGTGGAGGAGTGGTTAGAAGATATGGAGCATGT 6 0.15 No Hit CTTTGACGCCGCGGTGCGCGTACCCGCCGCCGGACCGGAGGGTGCAGAAG 6 0.15 No Hit GACGACGGCGACTGCGCGGCGTTCTGGCAGAGGGAGTGGGAGCGGTGGAC 6 0.15 No Hit GCCGGTGCAGTTCACTTTGACGCCGCGGTGCGCGTACCCGCCGCCGGACC 6 0.15 No Hit CCCGGAGGCGCAGATATATGTCGGGCTGCCGGCGTCGGAGCAGAAGGTTG 5 0.125 No Hit GCTTGTGTGGTTCCTTTAATCACTGTCACTGTGTGTGAATTTGCTGTCAT 5 0.125 No Hit AAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGATGG 5 0.125 No Hit GGGCACCCGGAGGCGCAGATATATGTCGGGCTGCCGGCGTCGGAGCAGAA 5 0.125 No Hit GGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTGCCGGCGTCG 5 0.125 No Hit TAGTAATAAAGAGCGGCATGGCTGGATCAGCGGAAGGAAAGGCAATTTTG 5 0.125 No Hit GTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTGCCGGCGT 5 0.125 No Hit AGAGGGAGTGGGAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATAT 5 0.125 No Hit ATTTGAAGCATTATCATTCCAAGAACCAATTCCTGATCCTTTGCGCGGCA 5 0.125 No Hit AGCTCTTCGACGACGACACCACGCCGGAGGTCCCCGTGCGCTCCGAGGCC 5 0.125 No Hit GGGAGTGGGAGCGGTGGACGGCGGGGCACCCGGAGGCGCAGATATATGTC 5 0.125 No Hit GCACCCCAAGAACTTGTTCTATGGTGTCATCCCCGTCGTGCAGAAGGCGT 5 0.125 No Hit ACCGGGGCCGGACGCCGGTGCAGTTCACTTTGACGCCGCGGTGCGCGTAC 5 0.125 No Hit CTTCGTCAGGTTCTACGACGACGGCGACTGCGCGGCGTTCTGGCAGAGGG 5 0.125 No Hit TTGAAGATAAGAGGACCAACTACAGTGGCTATGCTATCACCCTGGCTTGA 5 0.125 No Hit GCCGGCGTCGGAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCT 5 0.125 No Hit GCGGCGTTCTGGCAGAGGGAGTGGGAGCGGTGGACGGCGGGGCACCCGGA 5 0.125 No Hit CTTGAGCTTGTGTGGTTCCTTTAATCACTGTCACTGTGTGTGAATTTGCT 5 0.125 No Hit ACAGTTGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAGACA 5 0.125 No Hit GGCGACTGCGCGGCGTTCTGGCAGAGGGAGTGGGAGCGGTGGACGGCGGG 5 0.125 No Hit AGCCATAGCTACAGCTCCGGGGAATTCTACGACGAGCTGGCCAGGAAGCT 5 0.125 No Hit ATTTTGATTCAGCTCTGGCATAACTGAGGATCCGTGTGTACTGATTGTCT 5 0.125 No Hit GCGGCATGGCTGGATCAGCGGAAGGAAAGGCAATTTTGCCTGACCATACT 5 0.125 No Hit TTATGATTTGGGAGCGGTTTGAAGATAAGAGGACCAACTACAGTGGCTAT 5 0.125 No Hit GCGGGGCACCCGGAGGCGCAGATATATGTCGGGCTGCCGGCGTCGGAGCA 5 0.125 No Hit CCTACATGCTCGGCTCCAACCCTGCCGTACCCCGCCCTTTCGGCGACGCC 5 0.125 No Hit ATTCTACGACGAGCTGGCCAGGAAGCTGTACGCGCACAACAAGGACTACC 5 0.125 No Hit GTTAGAAGATATGGAGCATGTATCCAGCAAAACAGGCAACGTCACTTTAG 5 0.125 No Hit CGTCAGGTTCTACGACGACGGCGACTGCGCGGCGTTCTGGCAGAGGGAGT 5 0.125 No Hit GCATCGCCCACAACCTCCTCCACCGTGGCTTCGCCGGCACCACATTCTTC 5 0.125 No Hit GTCGGAGCAGAAGGTTGGGTATGTGCACCCCAAGAACTTGTTCTATGGTG 5 0.125 No Hit AGGCGTCGAATTATGGTGGTATTATGATTTGGGAGCGGTTTGAAGATAAG 5 0.125 No Hit ATTGTCTGGTTGAAGGATCTGCATTTTGATTCAGCTCTGGCATAACTGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGATC 165 0.0023855222 21.630682 2 GGTAGAT 165 0.0023855222 21.630682 1 TAGATCA 165 0.0023855222 21.630682 3 GATCATC 170 0.0028376877 20.994486 5 ATCATCG 170 0.0028376877 20.994486 6 TCATCGA 170 0.0028376877 20.994486 7 AGATCAT 170 0.0028376877 20.994486 4 ATCGATC 175 0.0033580794 20.394644 9 CATCGAT 185 0.004635286 19.29223 8 GGATGTG 60 0.0032904265 15.1875 135-139 GTGGATG 65 0.005602326 14.019231 135-139 TGTGGAA 65 0.0070295767 13.550722 125-129 TGTGGAT 70 0.009150563 13.017857 135-139 ATGAATG 95 0.0071575744 10.706709 120-124 CAGTTAT 95 0.007577235 10.625598 115-119 GTTTGCA 95 0.008169375 10.519342 110-114 ATGTGGA 135 7.712921E-4 9.786633 125-129 GAATGTG 155 2.5317282E-4 9.470935 125-129 AATGTGG 145 0.0014842122 9.111691 125-129 >>END_MODULE Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra Read 1498004 spots for ERR5262785.sra Written 1498004 spots for ERR5262785.sra Read 1498003 spots for ERR5262785.sra Written 1498003 spots for ERR5262785.sra SRR ids: ['ERR5262785.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1gm6hsrx ERR5262785.sra spots: 29960061 blocks: [[1, 1498003], [1498004, 2996006], [2996007, 4494009], [4494010, 5992012], [5992013, 7490015], [7490016, 8988018], [8988019, 10486021], [10486022, 11984024], [11984025, 13482027], [13482028, 14980030], [14980031, 16478033], [16478034, 17976036], [17976037, 19474039], [19474040, 20972042], [20972043, 22470045], [22470046, 23968048], [23968049, 25466051], [25466052, 26964054], [26964055, 28462057], [28462058, 29960061]] ERR5262785 file size 9861324 ERR5262785 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262785 ERR5262785_1.fastq ERR5262785_2.fastq Input file: ERR5262785_1.fastq Paired file: ERR5262785_2.fastq trimmed: ERR5262785-trimmed-pair1.fastq, ERR5262785-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:32:09 2024 >> started Fri Dec 6 11:32:41 2024 >> done (31.802s) 29960061 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 29960061 (100.00%) read pairs available; of these: 9043 ( 0.03%) trimmed read pairs available after processing 29951018 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 1 0.00% 22 0 0.00% 23 0 0.00% 24 2 0.00% 25 0 0.00% 26 2 0.00% 27 0 0.00% 28 3 0.00% 29 2 0.00% 30 3 0.00% 31 3 0.00% 32 3 0.00% 33 4 0.00% 34 5 0.00% 35 1 0.00% 36 1 0.00% 37 3 0.00% 38 4 0.00% 39 2 0.00% 40 2 0.00% 41 1 0.00% 42 0 0.00% 43 1 0.00% 44 1 0.00% 45 4 0.00% 46 2 0.00% 47 0 0.00% 48 2 0.00% 49 347 0.00% 50 347 0.00% 51 347 0.00% 52 409 0.00% 53 454 0.00% 54 505 0.00% 55 549 0.00% 56 564 0.00% 57 671 0.00% 58 741 0.00% 59 796 0.00% 60 848 0.00% 61 1018 0.00% 62 1103 0.00% 63 1195 0.00% 64 1327 0.00% 65 1489 0.00% 66 1664 0.01% 67 1837 0.01% 68 1969 0.01% 69 2157 0.01% 70 2688 0.01% 71 2978 0.01% 72 3376 0.01% 73 3735 0.01% 74 4197 0.01% 75 4588 0.02% 76 4913 0.02% 77 5452 0.02% 78 5851 0.02% 79 6853 0.02% 80 7674 0.03% 81 8363 0.03% 82 9430 0.03% 83 10678 0.04% 84 11785 0.04% 85 12600 0.04% 86 13437 0.04% 87 14396 0.05% 88 15316 0.05% 89 16499 0.06% 90 18216 0.06% 91 19821 0.07% 92 21494 0.07% 93 23700 0.08% 94 25097 0.08% 95 26542 0.09% 96 28010 0.09% 97 28893 0.10% 98 29748 0.10% 99 31244 0.10% 100 33598 0.11% 101 35051 0.12% 102 37812 0.13% 103 40362 0.13% 104 41795 0.14% 105 43996 0.15% 106 45718 0.15% 107 45976 0.15% 108 48029 0.16% 109 49573 0.17% 110 51119 0.17% 111 53369 0.18% 112 55591 0.19% 113 58309 0.19% 114 60972 0.20% 115 62639 0.21% 116 64627 0.22% 117 65614 0.22% 118 67212 0.22% 119 68326 0.23% 120 69986 0.23% 121 71592 0.24% 122 72947 0.24% 123 76390 0.25% 124 79703 0.27% 125 80476 0.27% 126 82720 0.28% 127 83127 0.28% 128 83838 0.28% 129 86109 0.29% 130 86298 0.29% 131 87724 0.29% 132 91658 0.31% 133 92950 0.31% 134 94196 0.31% 135 96810 0.32% 136 99171 0.33% 137 99438 0.33% 138 100490 0.34% 139 103834 0.35% 140 106217 0.35% 141 113042 0.38% 142 115180 0.38% 143 118543 0.40% 144 121093 0.40% 145 118636 0.40% 146 123903 0.41% 147 252501 0.84% 148 109070 0.36% 149 108324 0.36% 150 25466443 85.00% 29960061 reads passed initial QC criterion=sequence-density sequence-density=3.14 sequence-density-rank=1 fanout-score=2.18 fanout-score-rank=30 prefix-density=3.45 prefix-fanout=2.0 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=121.31 fanout-score-rank=1 prefix-density=1.04 prefix-fanout=1.0 sequence=CCATTGGAGCAATTGAGTCAGAAAACAGCACCGGAGAAGGAGCAGAGAGGAGGGCAAGAAGCAAGACCACAGAACAGGTTGATTATAGCGAAGCAGCTAGCCAGAGAGATTGGTGGAAGAAAAGAGTCAGAATAAAATTGCAGAGAAATCCTAAACTACGGGAAGGGGAAGCCAAAAGCGATATCAGGATGGCTTCTTCGCGCAGTTCGAGTACGCAGCAGAAAACTTCTTGGCACCTCCAGCTGGTCGAACGTCCTCAATGCTGTAACCGAGGGGAGCTTCGTACATCAGGGAACTACTACTGCTAGACTTTTTGCCACCCTTTGATTCCATTAGTACATTCACTTAAGTTCCTGGGAAAAATTGTTTGGTTCCCCCAAA criterion=sequence-density sequence-density=5.84 sequence-density-rank=1 fanout-score=2.04 fanout-score-rank=25 prefix-density=5.87 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=24 fanout-score=44.14 fanout-score-rank=1 prefix-density=2.52 prefix-fanout=1.5 sequence=GACAACTGCAACGATGCTGGCAGGAGTGTCATGGAAAGATACACTAAGATGAGCAACGCCATGAAGACATACGGGAAGAACATCTTCTTCTCGCTCTGTGAATGGGGGAAAGAAAACCCTGCTACCTGGGGTGCTGGTATGGGTAACAGCTGGAGAACAACCGCTGACATCGCCGACAACTGGGCCAGCATGACATCCTGTGCGGACCAGAACGACAGATGGGCCTCCTACGCCGGACCTGGTGGATGGAATGATCCTGACATGCTTGAAGTGGGGAACGGCGGGATGTCTGATGCTGAGTACCGTTCACACTTCAGCATCTGGGCCCTTGCCAAGGCTCCTCTTCTGATCGGGTGTGACGTGCGCACGATGAGCCAGCAGACAAAGGACATACTCAGCAATGGGGAGGTCATCGCCGTCAACCAAGATTCACTGGGCGTCCAGGGAAAGAAAGTGCAATCGGACAACGGTCTGGAGGTTTGGGCCGGTCCGCTCAGTGGCAACAGGAAGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262785 ERR5262785_1.fastq ERR5262785_2.fastq Input file: ERR5262785_1.fastq Paired file: ERR5262785_2.fastq trimmed: ERR5262785-trimmed-pair1.fastq, ERR5262785-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:36:11 2024 >> started Fri Dec 6 11:36:28 2024 >> done (17.247s) 17976037 read pairs processed; of these: 123 ( 0.00%) short read pairs filtered out after trimming by size control 418 ( 0.00%) empty read pairs filtered out after trimming by size control 17975496 (100.00%) read pairs available; of these: 157 ( 0.00%) trimmed read pairs available after processing 17975339 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 0 0.00% 24 1 0.00% 25 0 0.00% 26 2 0.00% 27 0 0.00% 28 3 0.00% 29 2 0.00% 30 2 0.00% 31 2 0.00% 32 0 0.00% 33 2 0.00% 34 1 0.00% 35 1 0.00% 36 0 0.00% 37 1 0.00% 38 4 0.00% 39 2 0.00% 40 1 0.00% 41 0 0.00% 42 0 0.00% 43 0 0.00% 44 1 0.00% 45 4 0.00% 46 2 0.00% 47 0 0.00% 48 2 0.00% 49 319 0.00% 50 314 0.00% 51 145 0.00% 52 291 0.00% 53 48 0.00% 54 77 0.00% 55 137 0.00% 56 518 0.00% 57 617 0.00% 58 576 0.00% 59 101 0.00% 60 112 0.00% 61 320 0.00% 62 905 0.01% 63 1127 0.01% 64 1148 0.01% 65 341 0.00% 66 542 0.00% 67 1710 0.01% 68 1831 0.01% 69 2016 0.01% 70 1023 0.01% 71 2193 0.01% 72 1615 0.01% 73 2058 0.01% 74 3419 0.02% 75 2440 0.01% 76 2210 0.01% 77 3256 0.02% 78 3404 0.02% 79 3424 0.02% 80 5048 0.03% 81 5755 0.03% 82 5751 0.03% 83 6131 0.03% 84 6722 0.04% 85 6837 0.04% 86 10067 0.06% 87 8092 0.05% 88 8065 0.04% 89 10626 0.06% 90 11503 0.06% 91 10349 0.06% 92 14065 0.08% 93 13343 0.07% 94 14781 0.08% 95 16273 0.09% 96 16904 0.09% 97 17440 0.10% 98 18376 0.10% 99 17829 0.10% 100 20597 0.11% 101 21634 0.12% 102 21676 0.12% 103 24521 0.14% 104 26466 0.15% 105 25960 0.14% 106 27964 0.16% 107 26384 0.15% 108 28500 0.16% 109 30316 0.17% 110 29740 0.17% 111 32089 0.18% 112 32875 0.18% 113 36363 0.20% 114 35572 0.20% 115 38430 0.21% 116 39889 0.22% 117 39697 0.22% 118 39726 0.22% 119 41871 0.23% 120 40130 0.22% 121 42207 0.23% 122 42698 0.24% 123 45429 0.25% 124 47309 0.26% 125 48245 0.27% 126 49853 0.28% 127 49611 0.28% 128 50002 0.28% 129 51647 0.29% 130 51362 0.29% 131 52210 0.29% 132 54753 0.30% 133 55869 0.31% 134 56880 0.32% 135 57436 0.32% 136 58630 0.33% 137 59408 0.33% 138 59898 0.33% 139 62200 0.35% 140 63682 0.35% 141 67393 0.37% 142 69007 0.38% 143 71350 0.40% 144 72553 0.40% 145 71549 0.40% 146 74223 0.41% 147 151094 0.84% 148 65385 0.36% 149 64826 0.36% 150 15286159 85.04% criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=4.60 fanout-score-rank=29 prefix-density=0.33 prefix-fanout=3.2 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.06 sequence-density-rank=32 fanout-score=189.11 fanout-score-rank=1 prefix-density=0.58 prefix-fanout=18.6 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCT criterion=sequence-density sequence-density=1.09 sequence-density-rank=1 fanout-score=2.08 fanout-score-rank=35 prefix-density=1.11 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=27 fanout-score=218.78 fanout-score-rank=1 prefix-density=1.03 prefix-fanout=17.5 sequence=GCCGCCGCCGTAGCAACCTTTGAGAGAACGAGATCTGCAAACCCGTTCTGAGGGAGAGGAAATTTCGTCGTCTCTGTTCGAGATCTGTGTCGAGAGAGGAGGGGGTTCAAAGGGGGAGTTCCAGAGATCGGAATCAGATCAAGCGGGCGCGCTCGGGATTTGGGGGAACCAAACAATTTTTCCCAGGAACTTAAGTGAATGTACTAATGGAATCAAAGGGTGGCAAAAAGTCTAGCAGTAGTAGTTCCCTGATGTACGAAGCTCCCCTCGGTTACAGCATTGAGGACGTTCGACCAGCTGGAGGTGCCAAGAAGTTTTCTGCTGCGTACTCGAACTGCGCGAAGAAGCCATCCTGATATCGCTTTTGGCTTCCCCTTCCCGTAGTTTAGGATTTCTCTGCAATTTTATTCTGACTCTTTTCTTCCACCAATCTCTCTGGCTAGCTGCTTCGCTATAATCAACCTGTTCTGTGGTCTTGCTTCTTGCCCTCCTCTCTGCTCCTTCTCCGGTG ERR5262785 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:37:40 Started mapping on | Dec 06 11:37:40 Finished on | Dec 06 11:40:13 Mapping speed, Million of reads per hour | 704.93 Number of input reads | 29959520 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 28611247 Uniquely mapped reads % | 95.50% Average mapped length | 291.70 Number of splices: Total | 26009288 Number of splices: Annotated (sjdb) | 24192245 Number of splices: GT/AG | 25635968 Number of splices: GC/AG | 312158 Number of splices: AT/AC | 15629 Number of splices: Non-canonical | 45533 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.23 Insertion rate per base | 0.01% Insertion average length | 2.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 340888 % of reads mapped to multiple loci | 1.14% Number of reads mapped to too many loci | 839 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.35% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1007385 1007385 1007385 N_multimapping 340888 340888 340888 N_noFeature 1083052 27839813 1341087 N_ambiguous 603852 3935 90419 UnstrandedReadsAssigned:26924343 PositiveStrandReadsAssigned:767499 NegativeStrandReadsAssigned:27179741 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262785 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262785-trimmed-pair1.fastq ERR5262785-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,959,520 reads, 27,707,843 reads pseudoaligned [quant] estimated average fragment length: 267.234 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,190 rounds 52973 ERR5262785.ke.tsv 35125 ERR5262785.se.tsv 88098 total ==> ERR5262785.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 670.395 0 0 PNS24247 1044 777.766 190.15 12.5328 PNS24249 1928 1661.77 390.773 12.0546 PNS24246 1044 777.766 190.15 12.5328 PNS24248 1044 777.766 190.15 12.5328 PNS24244 1471 1204.77 233.777 9.94715 PNS24243 293 103.387 0 0 KQK14069 1603 1336.77 48190.5 1848.01 KQK14071 474 238.319 89.575 19.2676 ==> ERR5262785.se.tsv <== BRADI_1g14170v3 48080 BRADI_1g53295v3 305 BRADI_1g59795v3 522 BRADI_1g07683v3 0 BRADI_1g00485v3 31 BRADI_1g20270v3 1131 BRADI_1g74790v3 1930 BRADI_1g09890v3 0 BRADI_1g77505v3 288 BRADI_1g48960v3 0 ERR5262785 completed mapping pipeline successfully