Starting /dee2/code/volunteer_pipeline.sh ERR5262786 current disk space = 1551500853248 free memory = 1422419008 ERR5262786 SRAfilesize a63e8872753f7ad69191c5162fc8f3ec ERR5262786.sra ERR5262786.sra file validated ERR5262786 is paired end ERR5262786 is conventional basespace ERR5262786 read1 length is 63-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262786_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 63-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6235 37.0 37.0 37.0 37.0 37.0 2 36.667 37.0 37.0 37.0 37.0 37.0 3 36.6985 37.0 37.0 37.0 37.0 37.0 4 36.773 37.0 37.0 37.0 37.0 37.0 5 36.795 37.0 37.0 37.0 37.0 37.0 6 36.778 37.0 37.0 37.0 37.0 37.0 7 36.7065 37.0 37.0 37.0 37.0 37.0 8 36.748 37.0 37.0 37.0 37.0 37.0 9 36.7475 37.0 37.0 37.0 37.0 37.0 10-14 36.70399999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.698100000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.6101 37.0 37.0 37.0 37.0 37.0 25-29 36.5806 37.0 37.0 37.0 37.0 37.0 30-34 36.5926 37.0 37.0 37.0 37.0 37.0 35-39 36.406499999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.38009999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.3708 37.0 37.0 37.0 37.0 37.0 50-54 36.2556 37.0 37.0 37.0 37.0 37.0 55-59 36.1834 37.0 37.0 37.0 37.0 37.0 60-64 36.13975166291573 37.0 37.0 37.0 37.0 37.0 65-69 36.01225306326582 37.0 37.0 37.0 37.0 37.0 70-74 35.85221305326332 37.0 37.0 37.0 37.0 37.0 75-79 35.924020124590925 37.0 37.0 37.0 37.0 37.0 80-84 35.70647349876772 37.0 37.0 37.0 37.0 37.0 85-89 35.94804804804805 37.0 37.0 37.0 37.0 37.0 90-94 35.85469711073116 37.0 37.0 37.0 37.0 37.0 95-99 35.71006509764647 37.0 37.0 37.0 37.0 37.0 100-104 35.97645049503784 37.0 37.0 37.0 37.0 37.0 105-109 35.92565042115278 37.0 37.0 37.0 37.0 37.0 110-114 35.713599211129555 37.0 37.0 37.0 37.0 37.0 115-119 35.766938475644146 37.0 37.0 37.0 37.0 37.0 120-124 35.854565180911194 37.0 37.0 37.0 37.0 37.0 125-129 35.74567783287143 37.0 37.0 37.0 37.0 37.0 130-134 35.73250022111885 37.0 37.0 37.0 37.0 37.0 135-139 35.622952353187095 37.0 37.0 37.0 37.0 37.0 140-144 35.488398003798466 37.0 37.0 37.0 37.0 37.0 145-149 35.50863863149093 37.0 37.0 37.0 37.0 37.0 150 35.471655328798185 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 2.0 24 1.0 25 6.0 26 3.0 27 4.0 28 6.0 29 16.0 30 15.0 31 41.0 32 60.0 33 123.0 34 227.0 35 536.0 36 2592.0 37 367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.825 13.575000000000001 6.800000000000001 39.800000000000004 2 18.275 10.75 32.65 38.324999999999996 3 16.85 18.125 22.225 42.8 4 19.3 24.675 22.425 33.6 5 19.875 27.250000000000004 30.375000000000004 22.5 6 19.950000000000003 42.575 17.724999999999998 19.75 7 16.225 29.775000000000002 37.5 16.5 8 19.575 30.4 20.4 29.625 9 16.225 23.674999999999997 32.975 27.125 10-14 14.825 29.325000000000003 24.93 30.919999999999998 15-19 19.8 24.51 24.335 31.355 20-24 19.005 25.605 24.585 30.805 25-29 19.245 27.139999999999997 25.355 28.26 30-34 17.445 25.465 27.77 29.32 35-39 17.36 29.2 26.945000000000004 26.495 40-44 18.105 29.330000000000002 26.945000000000004 25.619999999999997 45-49 16.905 30.409999999999997 27.295 25.39 50-54 20.51 27.52 28.435 23.535 55-59 18.93 32.855000000000004 25.53 22.685 60-64 17.485874293714684 31.66658332916646 27.12635631781589 23.721186059302966 65-69 20.080020005001252 31.037759439859965 29.40735183795949 19.474868717179294 70-74 18.3745936484121 32.23305826456614 26.486621655413856 22.9057264316079 75-79 19.573808213696164 30.318643389525285 25.3564103846731 24.751138012105447 80-84 18.444755804643716 26.621297037630104 30.004003202562053 24.92994395516413 85-89 19.824824824824823 28.773773773773776 27.81781781781782 23.583583583583582 90-94 18.15359967958346 27.205366977070188 31.255632322018624 23.385401021327727 95-99 17.84677015523285 28.68803204807211 29.68953430145218 23.775663495242863 100-104 21.397073561836038 25.992182802164766 27.640809781519344 24.969933854479855 105-109 20.926148906281355 25.978326309452136 29.42504515352197 23.67047963074453 110-114 19.231741218094516 27.167045741723527 30.983067980793532 22.618145059388425 115-119 22.85933503836317 26.189258312020456 26.47058823529412 24.48081841432225 120-124 21.51225806451613 25.409032258064517 27.912258064516127 25.166451612903224 125-129 23.489897937929598 29.634451156009167 23.666944386586128 23.208706519475108 130-134 24.278795535902294 25.77911139187197 24.8210149505159 25.121078121709832 135-139 20.734571855648092 27.087337177023272 28.518043988896007 23.66004697843263 140-144 18.790225809970547 25.329988000436348 28.2807897894622 27.598996400130904 145-149 20.329116758087988 26.849882458300684 25.99350722041867 26.827493563192657 150 18.820861678004537 29.903628117913833 25.595238095238095 25.68027210884354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 0.5 24 0.5 25 1.5 26 1.5 27 1.5 28 2.0 29 4.5 30 8.0 31 5.0 32 8.0 33 17.5 34 33.0 35 39.5 36 36.0 37 43.5 38 77.5 39 125.0 40 185.0 41 209.5 42 232.0 43 349.5 44 591.5 45 623.5 46 379.5 47 204.5 48 104.0 49 68.5 50 62.0 51 36.0 52 24.5 53 26.0 54 57.0 55 205.0 56 168.5 57 15.0 58 5.0 59 0.5 60 1.5 61 3.0 62 16.5 63 15.0 64 1.5 65 1.5 66 1.5 67 1.0 68 2.5 69 3.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 62-63 1.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 1.0 80-81 0.0 82-83 1.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.0 100-101 1.0 102-103 1.0 104-105 1.0 106-107 3.0 108-109 12.0 110-111 15.0 112-113 23.0 114-115 21.0 116-117 10.0 118-119 12.0 120-121 16.0 122-123 22.0 124-125 9.0 126-127 10.0 128-129 14.0 130-131 26.0 132-133 20.0 134-135 19.0 136-137 18.0 138-139 42.0 140-141 37.0 142-143 21.0 144-145 49.0 146-147 42.0 148-149 20.0 150-151 3528.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.15 #Duplication Level Percentage of deduplicated Percentage of total 1 40.57971014492754 9.8 2 17.805383022774325 8.6 3 13.457556935817806 9.75 4 8.592132505175984 8.3 5 3.6231884057971016 4.375 6 2.587991718426501 3.75 7 1.3457556935817805 2.275 8 1.5527950310559007 3.0 9 1.8633540372670807 4.05 >10 7.867494824016563 31.574999999999996 >50 0.6211180124223602 10.775 >100 0.10351966873706005 3.75 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACAC 150 3.75 No Hit GTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAG 88 2.1999999999999997 No Hit GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 87 2.175 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 81 2.025 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 64 1.6 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 58 1.4500000000000002 No Hit GGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAG 53 1.325 No Hit GCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACA 49 1.225 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 43 1.075 No Hit CCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTT 33 0.8250000000000001 No Hit GTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAAT 33 0.8250000000000001 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTT 32 0.8 No Hit CCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGC 28 0.7000000000000001 No Hit GCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGA 27 0.675 No Hit ACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCC 26 0.65 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 26 0.65 No Hit CTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATG 25 0.625 No Hit GCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTT 24 0.6 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 23 0.575 No Hit GCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCA 22 0.5499999999999999 No Hit GATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAGG 22 0.5499999999999999 No Hit CTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAA 21 0.525 No Hit CGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTT 21 0.525 No Hit CTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 21 0.525 No Hit CCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTT 20 0.5 No Hit TGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTG 20 0.5 No Hit GTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCG 20 0.5 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 20 0.5 No Hit CATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACG 19 0.475 No Hit ATCCTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGC 19 0.475 No Hit CCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCC 19 0.475 No Hit ATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCAT 18 0.44999999999999996 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 17 0.42500000000000004 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 17 0.42500000000000004 No Hit CGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCT 16 0.4 No Hit CTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCG 16 0.4 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACA 16 0.4 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 16 0.4 No Hit GTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCT 16 0.4 No Hit ACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTT 16 0.4 No Hit GGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAATTCGGAATGTTAT 16 0.4 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 15 0.375 No Hit CCTTGACACATCTGGTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCT 15 0.375 No Hit CTCCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGAC 15 0.375 No Hit CTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAG 14 0.35000000000000003 No Hit CACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATAA 14 0.35000000000000003 No Hit CCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAG 14 0.35000000000000003 No Hit CTGCGCGTTGCCGCCGCCCATGGCTTCTTTCTCCTCGGCGAGAACGAGAG 14 0.35000000000000003 No Hit CATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCA 13 0.325 No Hit CCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATC 13 0.325 No Hit GCCCCTTCCATCTCCTCAGAGATATCATCGTCATACTGAAGAATAACAGA 13 0.325 No Hit GGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCC 12 0.3 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 12 0.3 No Hit TGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGA 12 0.3 No Hit ATCTAATTTATATTTGTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGC 12 0.3 No Hit ACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGAT 12 0.3 No Hit GGCCAAACGCATTCAGACAAATGCATATCGGTCCCCTTGCTCTTGACTCT 12 0.3 No Hit CACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCAT 12 0.3 No Hit CATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCA 12 0.3 No Hit CCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTT 11 0.27499999999999997 No Hit CCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATA 11 0.27499999999999997 No Hit GTCAACAAGATTGATTGTGCCATCACTGATGGCTGTGTAAATCTTAACAC 11 0.27499999999999997 No Hit ATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAA 11 0.27499999999999997 No Hit GAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTAC 11 0.27499999999999997 No Hit CAGGCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAG 11 0.27499999999999997 No Hit GCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCT 11 0.27499999999999997 No Hit ACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTT 11 0.27499999999999997 No Hit GCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGC 11 0.27499999999999997 No Hit CTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGA 11 0.27499999999999997 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 10 0.25 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 10 0.25 No Hit CCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGCC 10 0.25 No Hit GGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCT 10 0.25 No Hit CCGGTAAATCTGCACGAAGAAACCGTTCAACTCTACGGAGGGGATCAGCT 10 0.25 No Hit ACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCA 10 0.25 No Hit CTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTT 10 0.25 No Hit GTGCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAA 10 0.25 No Hit GATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTT 10 0.25 No Hit CTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTT 10 0.25 No Hit CCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACAT 10 0.25 No Hit ACTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTT 10 0.25 No Hit CGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCG 10 0.25 No Hit GTCCATCAACGGCCCCTTCCATCTCCTCAGAGATATCATCGTCATACTGA 10 0.25 No Hit CACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCA 9 0.22499999999999998 No Hit CTCTTATTGAGTATACGTCCGGGTGATATTCTTCATCAAAAAGGCCTTGA 9 0.22499999999999998 No Hit GACCCTTATAGGCAGTTTTACTAAAGTGAGAAGTGGCACTACTGACTGCA 9 0.22499999999999998 No Hit CAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCC 9 0.22499999999999998 No Hit CCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGGAACCTGAGTTGC 9 0.22499999999999998 No Hit GGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAAATAAAAGG 9 0.22499999999999998 No Hit ATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAG 9 0.22499999999999998 No Hit GGACCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCACAGA 9 0.22499999999999998 No Hit CTGTGGACCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCA 9 0.22499999999999998 No Hit CCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAAT 9 0.22499999999999998 No Hit CTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCTAA 9 0.22499999999999998 No Hit CCAGAAAGTAATCCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGG 9 0.22499999999999998 No Hit CCTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCG 9 0.22499999999999998 No Hit ATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCT 9 0.22499999999999998 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 9 0.22499999999999998 No Hit ATGCACATGAGCTACCATTGTAGAAATCCTCAAACAGGAGGACGGAATGG 9 0.22499999999999998 No Hit CACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTT 9 0.22499999999999998 No Hit GCAGGATCGAACCTCCTGTATTAACTCTTAAACATCTGACATCTCTATCT 9 0.22499999999999998 No Hit CCACAAAATAATCATACTATTTCAGAGCTTCAGGCCTTCAGTGTCCAGAT 8 0.2 No Hit CCCTTCTCCAAGTTTTCCTTTCCCAGAAAGTAATCCCAAACCAAATGCTA 8 0.2 No Hit GTCACAGGCTAAGCATCATCATTTTTTCGCCGTGAGCCAAGAATCAAAAA 8 0.2 No Hit TGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATC 8 0.2 No Hit GACGGAATGGCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCA 8 0.2 No Hit GTCCTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGC 8 0.2 No Hit CTTCAGAATCCAGTGTTGTCCTTGGCAGTTGCCTACGGAGCAAAACGATT 8 0.2 No Hit CATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAAC 8 0.2 No Hit CCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCACAGAGGG 8 0.2 No Hit CCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGACAG 8 0.2 No Hit GCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGC 8 0.2 No Hit AGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 8 0.2 No Hit CCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTG 8 0.2 No Hit CTGTAAGACTTTTTGAATTCCAGAAAGAGCCATACGGCTAATTGATATCC 8 0.2 No Hit GATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAAC 8 0.2 No Hit GGCCTGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCA 7 0.17500000000000002 No Hit TCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTT 7 0.17500000000000002 No Hit GCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCA 7 0.17500000000000002 No Hit GCTATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACA 7 0.17500000000000002 No Hit CAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAATTC 7 0.17500000000000002 No Hit CTCATTAAAACCTCCCGGTAAATCTGCACGAAGAAACCGTTCAACTCTAC 7 0.17500000000000002 No Hit CTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTC 7 0.17500000000000002 No Hit GCACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCTTG 7 0.17500000000000002 No Hit CGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGC 7 0.17500000000000002 No Hit AGCTGCCCTACACGAACCCTTGACACATCTGGTGGGCATAGAGCGCAGAG 7 0.17500000000000002 No Hit CAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAAAT 7 0.17500000000000002 No Hit GTCCCCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCTGACAAATC 7 0.17500000000000002 No Hit AGGTCACAGGCTAAGCATCATCATTTTTTCGCCGTGAGCCAAGAATCAAA 7 0.17500000000000002 No Hit GCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCA 6 0.15 No Hit CTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 6 0.15 No Hit CTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGACAGA 6 0.15 No Hit CCCAGAAAGTAATCCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAG 6 0.15 No Hit CACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACG 6 0.15 No Hit GCCCCTGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 6 0.15 No Hit GCGTGCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGC 6 0.15 No Hit GCCCGTGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 6 0.15 No Hit ATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGG 6 0.15 No Hit CTTGTCAACAAGATTGATTGTGCCATCACTGATGGCTGTGTAAATCTTAA 6 0.15 No Hit CTTGATAATTATAAGAACAGTGAAAATTCTCGCGACAAACCTATCCAGGG 6 0.15 No Hit GGCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGC 6 0.15 No Hit GCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTT 6 0.15 No Hit CTTCCATCTCCTCAGAGATATCATCGTCATACTGAAGAATAACAGAACAA 6 0.15 No Hit GTTCCACAAAATAATCATACTATTTCAGAGCTTCAGGCCTTCAGTGTCCA 6 0.15 No Hit ACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATA 6 0.15 No Hit ATTTGTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGC 6 0.15 No Hit CTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGCA 6 0.15 No Hit CGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGT 6 0.15 No Hit GACACATCTGGTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATG 6 0.15 No Hit ACACTCTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATA 6 0.15 No Hit ATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTT 6 0.15 No Hit GTCCCTAAGTTTCTGGTCTCCTCTGAATCCCGTTGCTAGTATTATAATAT 6 0.15 No Hit CATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGC 6 0.15 No Hit GACTTTTTGAATTCCAGAAAGAGCCATACGGCTAATTGATATCCATCTTG 6 0.15 No Hit GTCCGCAGTTGGCCAAACGCATTCAGACAAATGCATATCGGTCCCCTTGC 5 0.125 No Hit CTCGATCCATCAGCAGCTTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTT 5 0.125 No Hit ACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCT 5 0.125 No Hit CATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGC 5 0.125 No Hit GCCCTTAGTAATGGGATATTACATCGAGTGAGAACATCAAAAAGTGTTTT 5 0.125 No Hit GGTGAAAGCTTTGAATTGTACTACCTAGTATCTACATATCTAGTGCTCCC 5 0.125 No Hit GGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGT 5 0.125 No Hit CCAATTTATGGTATCCATTACAGGTCACAGGCTAAGCATCATCATTTTTT 5 0.125 No Hit GGGACGCACTGGAGGTAGGCGAGGCGGGGCACGTCGTCGAAGGACACCAG 5 0.125 No Hit CTATTTCAGAGCTTCAGGCCTTCAGTGTCCAGATCCAATTTATGGTATCC 5 0.125 No Hit CAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGG 5 0.125 No Hit GTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCA 5 0.125 No Hit GCCATTTTGGACAAGAAGGGCCTCTGTGCCCTCTAACAGACAATTTTTGA 5 0.125 No Hit CAGAAAGTAATCCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGGA 5 0.125 No Hit AGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAG 5 0.125 No Hit GGCTAATTTTTGGTTTTTGCAAGATCAGTGTAGCGTCAAGGTTCCCCAGC 5 0.125 No Hit GCTCGAGGAACCTGAGTTGCTCTTATTGAGTATACGTCCGGGTGATATTC 5 0.125 No Hit TTTTTTTTTGAAATAAATGGATGGATTTATCAGAGAAGCAACTGTCTACA 5 0.125 No Hit CCCTGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 5 0.125 No Hit ATAGCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATA 5 0.125 No Hit GCTCCTTCCATCTCCTCAGAGATATCATCGTCATACTGAAGAATAACAGA 5 0.125 No Hit CATCGGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAA 5 0.125 No Hit ATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTT 5 0.125 No Hit CTCCTCTTCTAGTATGAACTGCGGAAGCTGTGTTGTTTTACCACAACCTG 5 0.125 No Hit CCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCTA 5 0.125 No Hit TACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTT 5 0.125 No Hit TGTCCCTAAGTTTCTGGTCTCCTCTGAATCCCGTTGCTAGTATTATAATA 5 0.125 No Hit CTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTT 5 0.125 No Hit CTCGCCTCTAATCGGAACCCAACCTGAAATCTGAGGAATAATTTTTTTTG 5 0.125 No Hit ATCGCTTGTGGTACTGCCTCATACTAAAACAATCACCTCCATCCACATCT 5 0.125 No Hit CCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGC 5 0.125 No Hit GTCCTCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCTGACAAATC 5 0.125 No Hit CCGGCCCAGCCCTACCAAGCCTCATGCCGGCCCTAGGCCGGGAGGCTGGG 5 0.125 No Hit CCCTTCCATCTCCTCAGAGATATCATCGTCATACTGAAGAATAACAGAAC 5 0.125 No Hit CCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATATG 25 9.7068556E-4 84.667496 2 GCCATAT 25 9.7068556E-4 84.667496 1 CATATGT 25 9.7068556E-4 84.667496 3 GTACACA 30 0.0020020113 70.55625 8 TACACAC 30 0.0020020113 70.55625 9 ATATGTA 30 0.0020020113 70.55625 4 TGTACAC 30 0.0020020113 70.55625 7 TATGTAC 35 0.003689071 60.476784 5 GAAAACA 40 0.006259627 52.917187 4 ATGTACA 40 0.006259627 52.917187 6 TGAAAAC 40 0.006259627 52.917187 3 CTGCTCC 35 0.001897442 23.038774 140-144 TACTAGC 35 0.0022376797 22.398808 130-134 GAGAGTA 40 0.005693543 19.069256 125-129 GCCGGGA 40 0.0066483757 18.567434 120-124 GGCCGGC 40 0.006959121 18.421997 115-119 AGATCGG 40 0.007281675 18.278822 110-114 ATACTAG 40 0.008446624 17.817234 100-104 CGGAGCT 40 0.008953397 17.639063 90-94 ACATGAG 40 0.008953397 17.639063 20-24 >>END_MODULE ERR5262786 read2 length is 63-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262786_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 63-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.293 37.0 37.0 37.0 37.0 37.0 2 36.135 37.0 37.0 37.0 37.0 37.0 3 36.297 37.0 37.0 37.0 37.0 37.0 4 36.191 37.0 37.0 37.0 37.0 37.0 5 36.3955 37.0 37.0 37.0 37.0 37.0 6 36.267 37.0 37.0 37.0 37.0 37.0 7 36.289 37.0 37.0 37.0 37.0 37.0 8 36.3825 37.0 37.0 37.0 37.0 37.0 9 36.413 37.0 37.0 37.0 37.0 37.0 10-14 36.3948 37.0 37.0 37.0 37.0 37.0 15-19 36.4027 37.0 37.0 37.0 37.0 37.0 20-24 36.2959 37.0 37.0 37.0 37.0 37.0 25-29 36.206900000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.225 37.0 37.0 37.0 37.0 37.0 35-39 36.223499999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.153 37.0 37.0 37.0 37.0 37.0 45-49 36.23479999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.10809999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.1156 37.0 37.0 37.0 37.0 37.0 60-64 36.09134863715929 37.0 37.0 37.0 37.0 37.0 65-69 36.051662915728926 37.0 37.0 37.0 37.0 37.0 70-74 35.988647161790446 37.0 37.0 37.0 37.0 37.0 75-79 35.96292882625359 37.0 37.0 37.0 37.0 37.0 80-84 35.887299784147416 37.0 37.0 37.0 37.0 37.0 85-89 35.92122122122122 37.0 37.0 37.0 37.0 37.0 90-94 35.83176902490874 37.0 37.0 37.0 37.0 37.0 95-99 35.79969954932399 37.0 37.0 37.0 37.0 37.0 100-104 35.69391724230634 37.0 37.0 37.0 37.0 37.0 105-109 35.63462080869764 37.0 37.0 37.0 37.0 37.0 110-114 35.750351013241804 37.0 37.0 37.0 37.0 37.0 115-119 35.63785132293866 37.0 37.0 37.0 37.0 37.0 120-124 35.595619512223536 37.0 37.0 37.0 37.0 37.0 125-129 35.52348333180059 37.0 37.0 37.0 37.0 37.0 130-134 35.45367755213826 37.0 37.0 37.0 34.6 37.0 135-139 35.38063982800075 37.0 37.0 37.0 34.6 37.0 140-144 35.214485561185136 37.0 37.0 37.0 29.8 37.0 145-149 35.278848045863604 37.0 37.0 37.0 29.8 37.0 150 35.22863610639502 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 2.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 4.0 23 0.0 24 2.0 25 7.0 26 7.0 27 13.0 28 15.0 29 10.0 30 17.0 31 26.0 32 52.0 33 118.0 34 233.0 35 682.0 36 2583.0 37 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3 21.625 9.875 24.2 2 30.275000000000002 24.8 27.6 17.325 3 21.349999999999998 27.675 30.625000000000004 20.349999999999998 4 27.075 29.675 24.925 18.325 5 29.675 31.1 21.725 17.5 6 23.849999999999998 38.175 21.175 16.8 7 22.5 19.85 36.975 20.674999999999997 8 23.0 25.25 27.425 24.325 9 23.95 24.099999999999998 28.425 23.525 10-14 26.355 26.1 25.345000000000002 22.2 15-19 26.185000000000002 25.564999999999998 25.995 22.255 20-24 26.974999999999998 25.814999999999998 26.029999999999998 21.18 25-29 25.740000000000002 26.075 26.41 21.775 30-34 26.784999999999997 25.919999999999998 26.995 20.3 35-39 25.445 25.615 26.365 22.575 40-44 25.705 26.25 26.945000000000004 21.099999999999998 45-49 24.625 25.45 27.295 22.63 50-54 25.64 25.71 26.805 21.845 55-59 24.115000000000002 26.965 27.529999999999998 21.39 60-64 24.451222561128056 25.44627231361568 28.826441322066103 21.27606380319016 65-69 25.136284071017755 26.501625406351586 26.981745436359088 21.380345086271568 70-74 25.501375343835956 26.636659164791197 26.51162790697674 21.350337584396097 75-79 26.30683807713471 25.336401380621282 26.87209244159872 21.48466810064529 80-84 26.556244995996796 25.670536429143315 28.102481985588472 19.670736589271417 85-89 25.32032032032032 27.002002002002 26.601601601601605 21.076076076076074 90-94 25.49314108340843 26.148993691799337 27.701011314709124 20.656853910083107 95-99 26.33450175262894 25.02253380070105 27.456184276414625 21.186780170255386 100-104 26.17759069953899 25.641411104429746 27.264982962517536 20.91601523351373 105-109 26.01344571543247 25.857916917519567 27.744330724463172 20.384306642584786 110-114 25.761940864291127 26.40384129390953 27.37932777356583 20.45489006823351 115-119 25.043478260869566 27.145780051150897 26.475703324808187 21.335038363171353 120-124 24.769032258064517 25.883870967741935 28.32 21.02709677419355 125-129 25.515517600499898 26.69235575921683 27.754634451156008 20.037492189127263 130-134 26.55295851758265 27.226784586228682 26.41082333122763 19.809433564961044 135-139 25.086791646637824 27.51695775249693 26.913421994338517 20.48282860652673 140-144 25.034125034125033 26.47010647010647 27.28910728910729 21.206661206661206 145-149 24.49538719597428 28.683254123567238 26.715124405926755 20.10623427453173 150 29.796264855687603 25.636672325976228 25.891341256366722 18.675721561969443 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 0.0 25 1.0 26 1.5 27 1.0 28 0.5 29 4.0 30 8.5 31 7.5 32 14.0 33 17.5 34 11.5 35 16.0 36 50.0 37 61.5 38 73.0 39 134.0 40 192.5 41 240.5 42 257.0 43 258.5 44 297.0 45 272.0 46 188.0 47 161.0 48 158.0 49 128.0 50 121.0 51 125.0 52 114.5 53 145.5 54 135.5 55 110.5 56 130.5 57 101.0 58 66.0 59 78.5 60 72.5 61 44.5 62 37.5 63 39.0 64 32.5 65 22.5 66 12.5 67 10.5 68 9.5 69 9.5 70 6.5 71 6.0 72 7.5 73 3.0 74 0.5 75 0.5 76 1.0 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 62-63 1.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 1.0 80-81 0.0 82-83 1.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.0 100-101 1.0 102-103 1.0 104-105 1.0 106-107 3.0 108-109 12.0 110-111 15.0 112-113 23.0 114-115 21.0 116-117 10.0 118-119 12.0 120-121 16.0 122-123 22.0 124-125 9.0 126-127 10.0 128-129 14.0 130-131 26.0 132-133 20.0 134-135 20.0 136-137 20.0 138-139 42.0 140-141 38.0 142-143 21.0 144-145 39.0 146-147 42.0 148-149 20.0 150-151 3534.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.25 #Duplication Level Percentage of deduplicated Percentage of total 1 47.57575757575758 19.625 2 20.12121212121212 16.6 3 13.454545454545455 16.650000000000002 4 7.030303030303029 11.600000000000001 5 4.484848484848484 9.25 6 2.3030303030303028 5.7 7 1.3939393939393938 4.025 8 0.8484848484848486 2.8000000000000003 9 0.48484848484848486 1.7999999999999998 >10 2.3030303030303028 11.95 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 26 0.65 No Hit GAAAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCC 23 0.575 No Hit GTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTG 21 0.525 No Hit GGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAG 17 0.42500000000000004 No Hit GATGGATTCCAGGAGTCTTCAGTGACTTCTGCCATGCCAAAAAAAAGATG 15 0.375 No Hit GGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCC 13 0.325 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 13 0.325 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 13 0.325 No Hit CTGATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTA 13 0.325 No Hit GGAGAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGGTCCACCG 13 0.325 No Hit GCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGC 13 0.325 No Hit CTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTGCAATAGA 13 0.325 No Hit CAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCT 12 0.3 No Hit GGGAGAGAGCCTCGGGGGAAGGATCAAGAGAGGCTCGCCCGCGCGCGTGA 12 0.3 No Hit AAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCCTC 12 0.3 No Hit TGATTACGCATGGCAACATGGTGGCCACAACTGCTGCAGTCAGGACAATC 12 0.3 No Hit GGTTTGCAGGTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTC 12 0.3 No Hit GGATAGCAAGCTCCAAGCGTAATCCCTGGAAGTTTTGACCGGGTTGTAGA 12 0.3 No Hit GACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTC 12 0.3 No Hit AGGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCG 12 0.3 No Hit GTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTAT 12 0.3 No Hit GTATGATGTGGAGTCAGATCCTCCGAGGACTCGGGAACTTGATACTGTTG 11 0.27499999999999997 No Hit GGGAGAACTGTTGGTGCTGGAGTCATCGCTAAAGTTATGAGCGAATAATC 11 0.27499999999999997 No Hit GATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACT 11 0.27499999999999997 No Hit CAAGAATGTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTTCAG 11 0.27499999999999997 No Hit AGAAGATATCCTGACCTTGCGATGGATCCGGCAAGATCAAACATTCATGA 11 0.27499999999999997 No Hit CATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAA 11 0.27499999999999997 No Hit GTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAAC 11 0.27499999999999997 No Hit GACTGATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTG 10 0.25 No Hit GAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCG 10 0.25 No Hit GGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTG 10 0.25 No Hit CAAGGGGCTGGGAGAGAGCCTCGGGGGAAGGATCAAGAGAGGCTCGCCCG 10 0.25 No Hit GGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGAC 10 0.25 No Hit TGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGAT 10 0.25 No Hit GATATCCTGACCTTGCGATGGATCCGGCAAGATCAAACATTCATGATAGA 10 0.25 No Hit GGTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTT 10 0.25 No Hit GTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCGCCCTGTTG 10 0.25 No Hit GACACCAGACCTGCTGTGATTGTGGACAGACCCTCCAGTCATGCCCAATC 10 0.25 No Hit AAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTG 9 0.22499999999999998 No Hit AACCAAGAATGTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTT 9 0.22499999999999998 No Hit GCAAGCTCCAAGCGTAATCCCTGGAAGTTTTGACCGGGTTGTAGAGGCTG 9 0.22499999999999998 No Hit GTTTTGACCGGGTTGTAGAGGCTGCACTAGGCATCGATTTGCGTCATCGA 9 0.22499999999999998 No Hit CTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTGATACATCA 9 0.22499999999999998 No Hit GTGTGGTTCCAGCGATCGATGAGATAAACCTGGCACAGGCTAAGCTGCAG 9 0.22499999999999998 No Hit CCTACTCTTATGATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGC 9 0.22499999999999998 No Hit GGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCGG 9 0.22499999999999998 No Hit CAGGTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCG 8 0.2 No Hit CAAGGAGTTGGATAGGCTGGCGGTTCGTCTGGGAGCTCTTGTGAATGAGG 8 0.2 No Hit ATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCATT 8 0.2 No Hit GCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACA 8 0.2 No Hit CACGGATCGAGGAAGAGAGAGGATGATTGGACCCCTGTTTTGGAGCAAGG 8 0.2 No Hit GATAAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTAGCCA 8 0.2 No Hit CCTGGATGATGGATAGCAAGCTCCAAGCGTAATCCCTGGAAGTTTTGACC 8 0.2 No Hit GGCATCGATTTGCGTCATCGAGGTTTGCAGGTCCGGTGATCCACTGCTCC 8 0.2 No Hit TGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAATTTTGG 8 0.2 No Hit GGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTT 8 0.2 No Hit AGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTT 8 0.2 No Hit CTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAA 8 0.2 No Hit GATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGGTCAT 8 0.2 No Hit GTTGCTGAAGTGTTCACAGGTGCACCTGGAAAGTATGTTGAACTAAAGGA 8 0.2 No Hit CCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCATTGGCTCA 7 0.17500000000000002 No Hit GGATCATCGCAAATGGATGGCACAATGGAGACCACCCTGCTTCACCCTGG 7 0.17500000000000002 No Hit CCTGACTACAGCTTCTCCTTCGCTATGTCTTCCTGCTTGATTGCAATGCT 7 0.17500000000000002 No Hit TGTATACAAGTGGAAGTACAGGTCTGCCCAAGGGTGTAATGATTACGCAT 7 0.17500000000000002 No Hit GTTGCTATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAA 7 0.17500000000000002 No Hit GAATGTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTTCAGTGA 7 0.17500000000000002 No Hit GTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATG 7 0.17500000000000002 No Hit ATATATTCACTGATCATAGATCTGGTTCATCTGGTGGAAGGTCAGCTGGC 7 0.17500000000000002 No Hit GATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAA 7 0.17500000000000002 No Hit CTCAAGCAGGAGGAAATAGTCATCAGCGCTTCTATATTCATGTCCAGGGG 7 0.17500000000000002 No Hit GGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTT 7 0.17500000000000002 No Hit GCAAGATCAAACATTCATGATAGAATTATAGCTGCTGAAAAACGGGTCTC 7 0.17500000000000002 No Hit GGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAG 7 0.17500000000000002 No Hit TTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGAG 7 0.17500000000000002 No Hit AAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTC 7 0.17500000000000002 No Hit GCGTCATCGAGGTTTGCAGGTCCGGTGATCCACTGCTCCAAGATGGAGCA 7 0.17500000000000002 No Hit GGATGAGCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAA 7 0.17500000000000002 No Hit CATCGATTTGCGTCATCGAGGTTTGCAGGTCCGGTGATCCACTGCTCCAA 7 0.17500000000000002 No Hit TTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCT 7 0.17500000000000002 No Hit CAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCA 7 0.17500000000000002 No Hit GCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAG 7 0.17500000000000002 No Hit TGCTATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGA 7 0.17500000000000002 No Hit AATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGT 7 0.17500000000000002 No Hit AGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATG 6 0.15 No Hit GGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTG 6 0.15 No Hit ATTTGCGTCATCGAGGTTTGCAGGTCCGGTGATCCACTGCTCCAAGATGG 6 0.15 No Hit TTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCA 6 0.15 No Hit CCCTGGAAGTTTTGACCGGGTTGTAGAGGCTGCACTAGGCATCGATTTGC 6 0.15 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCGGCTCTGACTATGACTG 6 0.15 No Hit GGTGTATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACAT 6 0.15 No Hit ATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCGCCCTGTTGAGG 6 0.15 No Hit ATAAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAA 6 0.15 No Hit GTGAAAGGCGGTGAGCTGGTTAGTGAAGGCGAGGAAGAGTTGTTCAATTT 6 0.15 No Hit GGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGA 6 0.15 No Hit GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 6 0.15 No Hit CACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAA 6 0.15 No Hit GCGAATAATCTGTTACGAACTTTTTTTTGGCCTAGCGATTTTAGTAAACA 6 0.15 No Hit CGGTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCGCCCTGT 6 0.15 No Hit ATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGA 6 0.15 No Hit CGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTGCAATAGATG 6 0.15 No Hit GAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCT 6 0.15 No Hit TGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAG 6 0.15 No Hit GTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTAT 6 0.15 No Hit GTTGCAGAGTGTTAGGCATGTTATCTATATTGAGGATGAACCTGTTGAGG 6 0.15 No Hit CACTTGACAACATTATCTTTCCATGCGGTTGAGGAGTTGGGCAAAACATC 6 0.15 No Hit GTCCGTTCTCTCCCCCATCCCCTGCACACTATTTTTTCCTATGACTGGAA 6 0.15 No Hit GCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGTGGTCA 6 0.15 No Hit GGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACT 6 0.15 No Hit GTTTCCCAAGGGGCTGGGAGAGAGCCTCGGGGGAAGGATCAAGAGAGGCT 6 0.15 No Hit GGATCCGGCAAGATCAAACATTCATGATAGAATTATAGCTGCTGAAAAAC 6 0.15 No Hit GGTTTGCAGGTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGG 6 0.15 No Hit CGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCG 6 0.15 No Hit ACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAG 6 0.15 No Hit GTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCG 6 0.15 No Hit GTCCAGAAGGTTCTCCAGAATTACAAGAATCTTCAAGATATTATTGCCAT 6 0.15 No Hit GTTTGCAGGTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCG 6 0.15 No Hit CAAACATTCATGATAGAATTATAGCTGCTGAAAAACGGGTCTCAAATGAA 6 0.15 No Hit CACAGATTGAAAACCGTATCCTCTATGCTGCACGTGGAATCTTCAACAAA 6 0.15 No Hit ATTGTGGACAGACCCTCCAGTCATGCCCAATCTGTCGCACTCCCATCTCT 6 0.15 No Hit CGCAAATGGATGGCACAATGGAGACCACCCTGCTTCACCCTGGATGATGG 6 0.15 No Hit GTTGGGCAAAACATCTCATACAGATCCAAGATTACCATCAAGCAGTGATA 6 0.15 No Hit GTCGCACTCCCATCTCTACAAGAATAAAGCTTTATTAGCAGGTGGTGGTC 5 0.125 No Hit AGCAAGGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGC 5 0.125 No Hit ATGGATTCCAGGAGTCTTCAGTGACTTCTGCCATGCCAAAAAAAAGATGA 5 0.125 No Hit GGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGA 5 0.125 No Hit TGTGGAGTCAGATCCTCCGAGGACTCGGGAACTTGATACTGTTGGTGTGC 5 0.125 No Hit AGTTATTATGTATACAAGTGGAAGTACAGGTCTGCCCAAGGGTGTAATGA 5 0.125 No Hit ATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAAC 5 0.125 No Hit AAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTA 5 0.125 No Hit GATCAAGAGAGGCTCGCCCGCGCGCGTGATGAATTGATTAATACGGCGGC 5 0.125 No Hit GTGCGCACTGTTGTCTACTCACAATTTCTTGAGTCATACAAGAGTGTGAC 5 0.125 No Hit AGCTATAGAGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAG 5 0.125 No Hit GGCCACAACTGCTGCAGTCAGGACAATCATTCCTAAACTTGGCACGGGAG 5 0.125 No Hit CCTGCACACTATTTTTTCCTATGACTGGAATATGAAAACTTCTTTGTACT 5 0.125 No Hit TGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGC 5 0.125 No Hit TTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCG 5 0.125 No Hit GCCTGGTGAAGGATTTCTGCACTATATTTTGGCCAGCGCACTCGCTCCCT 5 0.125 No Hit GCTAAACAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTGT 5 0.125 No Hit CGCACTCCCATCTCTACAAGAATAAAGCTTTATTAGCAGGTGGTGGTCTG 5 0.125 No Hit GTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAAT 5 0.125 No Hit GGATGACGATCCTGCCCCAAGAGCTGATGTTTTTGCATTTGCTCAGAGCT 5 0.125 No Hit GTCGATCTCGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCCTTT 5 0.125 No Hit CATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGC 5 0.125 No Hit CGATTTGCGTCATCGAGGTTTGCAGGTCCGGTGATCCACTGCTCCAAGAT 5 0.125 No Hit ATTCGGGGCCGGAACGGAGACCACTTCAACGACGACGGAATGGGCGATGT 5 0.125 No Hit GATTACGCATGGCAACATGGTGGCCACAACTGCTGCAGTCAGGACAATCA 5 0.125 No Hit GATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTGCA 5 0.125 No Hit AATGATTACGCATGGCAACATGGTGGCCACAACTGCTGCAGTCAGGACAA 5 0.125 No Hit CTTGGATTGGGCATGCGGTCCTGGCTCCGCAAACTGCAGTGCAATTCAAC 5 0.125 No Hit GCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGC 5 0.125 No Hit GCAACTTGTACCAATGTTGTAAAAAGACAGTTGTGGTTTTCAACACATTC 5 0.125 No Hit GTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGC 5 0.125 No Hit GAGCTATTAGGAAAACGATGTTATGTTCGTCAGTCACCTATTTCGAATCA 5 0.125 No Hit GGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGG 5 0.125 No Hit CACGTTGGAGCAGCAGCAGAGAGCCGGAGCGCCACCAGCCATCCGATCAA 5 0.125 No Hit CAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATC 5 0.125 No Hit CCCAGCTGCTGGAAATGTTTGTTTTGCGAGTGGTTCTGCTGGCTGGTCTT 5 0.125 No Hit GCAAGCTATAGAGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGA 5 0.125 No Hit GGCGCGCGCAGTGACGAGATAAGATCGGGCGTGCGAGCGCGGGACCTTGT 5 0.125 No Hit TCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTTCAGTGACTTCTG 5 0.125 No Hit TATGTGCCGCTCGAGGATTCCGAGAAGCACAAGGTCGAGGGCGAGGTGCA 5 0.125 No Hit AGCAGTGATACCGCAGTTATTATGTATACAAGTGGAAGTACAGGTCTGCC 5 0.125 No Hit CTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTGCCC 5 0.125 No Hit CATATATTCACTGATCATAGATCTGGTTCATCTGGTGGAAGGTCAGCTGG 5 0.125 No Hit GGAAAATTCAGCGGTTCCTTAGCCAGCCTTTTCATGTTGCTGAAGTGTTC 5 0.125 No Hit GGCCGACCGGCGACCCAAGGAGTTCTCTATCTGACAAAAGAAATGTACAT 5 0.125 No Hit AGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGC 5 0.125 No Hit GAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGTGGTCAACCTGTTG 5 0.125 No Hit CAGCCTTCAATCATTCGTCCTTGTTGTTTGAGTACAAGAAGAGCCGTGAC 5 0.125 No Hit CGAGGTTTGCAGGTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGAAT 5 0.125 No Hit GTGTATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATG 5 0.125 No Hit ATGGCACAATGGAGACCACCCTGCTTCACCCTGGATGATGGATAGCAAGC 5 0.125 No Hit GCATCCACTTGTTTGTTGAAATATTGGACCATTTCTTCAGCAATGTATGT 5 0.125 No Hit GGCTGCACTAGGCATCGATTTGCGTCATCGAGGTTTGCAGGTCCGGTGAT 5 0.125 No Hit AGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGAT 5 0.125 No Hit GCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAAT 5 0.125 No Hit TAAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAAA 5 0.125 No Hit GGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAAT 5 0.125 No Hit CCAGTGTATCATCCTCCATGATGGATGCGATGAATAAAATTGACAAGGAG 5 0.125 No Hit GGACAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTT 5 0.125 No Hit AAGGGTAGGGTGAAAGGCGGTGAGCTGGTTAGTGAAGGCGAGGAAGAGTT 5 0.125 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 5 0.125 No Hit CTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTCAATTGTTTC 5 0.125 No Hit CTTGCTTTTATCACACCTGTGGTCTATGACTTCTACAACTACGACATGGA 5 0.125 No Hit AAATAAGATAAAGAAGGGGACAAAAGGAGACGTTTCTGTACTGAAACCCA 5 0.125 No Hit ATTCACTGATCATAGATCTGGTTCATCTGGTGGAAGGTCAGCTGGCTATG 5 0.125 No Hit GTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTTCAGTGACTTC 5 0.125 No Hit GAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGGTCCACCGCTC 5 0.125 No Hit CAGGACAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACC 5 0.125 No Hit CAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACT 5 0.125 No Hit AGTCATGCCCAATCTGTCGCACTCCCATCTCTACAAGAATAAAGCTTTAT 5 0.125 No Hit CAAGAATTCTCTTTTTATTTCTTGATTTGTGTGCAGCAGTTTTATTTGAA 5 0.125 No Hit CTCATAATTAGCCATGATCCTCCGCCGATCTTCACCATCCTGTCTCCACC 5 0.125 No Hit AATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCAT 5 0.125 No Hit TGTTGATCCTGTTGTGGAAGCAGTAGTTCTTGAGAGCCTTCAACCACATA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264856 spots for ERR5262786.sra Written 2264856 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra Read 2264853 spots for ERR5262786.sra Written 2264853 spots for ERR5262786.sra SRR ids: ['ERR5262786.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bxcwj0by ERR5262786.sra spots: 45297063 blocks: [[1, 2264853], [2264854, 4529706], [4529707, 6794559], [6794560, 9059412], [9059413, 11324265], [11324266, 13589118], [13589119, 15853971], [15853972, 18118824], [18118825, 20383677], [20383678, 22648530], [22648531, 24913383], [24913384, 27178236], [27178237, 29443089], [29443090, 31707942], [31707943, 33972795], [33972796, 36237648], [36237649, 38502501], [38502502, 40767354], [40767355, 43032207], [43032208, 45297063]] ERR5262786 file size 14839290 ERR5262786 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262786 ERR5262786_1.fastq ERR5262786_2.fastq Input file: ERR5262786_1.fastq Paired file: ERR5262786_2.fastq trimmed: ERR5262786-trimmed-pair1.fastq, ERR5262786-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:38:54 2024 >> started Fri Dec 6 11:39:44 2024 >> done (50.094s) 45297063 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 45297063 (100.00%) read pairs available; of these: 11723 ( 0.03%) trimmed read pairs available after processing 45285340 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 0 0.00% 21 2 0.00% 22 0 0.00% 23 1 0.00% 24 2 0.00% 25 2 0.00% 26 2 0.00% 27 4 0.00% 28 6 0.00% 29 5 0.00% 30 3 0.00% 31 2 0.00% 32 3 0.00% 33 6 0.00% 34 3 0.00% 35 3 0.00% 36 11 0.00% 37 4 0.00% 38 7 0.00% 39 9 0.00% 40 1 0.00% 41 2 0.00% 42 4 0.00% 43 1 0.00% 44 7 0.00% 45 6 0.00% 46 7 0.00% 47 3 0.00% 48 6 0.00% 49 460 0.00% 50 573 0.00% 51 598 0.00% 52 697 0.00% 53 665 0.00% 54 751 0.00% 55 843 0.00% 56 880 0.00% 57 1082 0.00% 58 1182 0.00% 59 1333 0.00% 60 1617 0.00% 61 1830 0.00% 62 2136 0.00% 63 2337 0.01% 64 2514 0.01% 65 2773 0.01% 66 3143 0.01% 67 3535 0.01% 68 3916 0.01% 69 4466 0.01% 70 5401 0.01% 71 6120 0.01% 72 6973 0.02% 73 7973 0.02% 74 8927 0.02% 75 9784 0.02% 76 10901 0.02% 77 11813 0.03% 78 12987 0.03% 79 14536 0.03% 80 16227 0.04% 81 18123 0.04% 82 20401 0.05% 83 22993 0.05% 84 24926 0.06% 85 27459 0.06% 86 29826 0.07% 87 31788 0.07% 88 34225 0.08% 89 36423 0.08% 90 39601 0.09% 91 42455 0.09% 92 45849 0.10% 93 48626 0.11% 94 53188 0.12% 95 56048 0.12% 96 58765 0.13% 97 61941 0.14% 98 63965 0.14% 99 66434 0.15% 100 69094 0.15% 101 72650 0.16% 102 76329 0.17% 103 80171 0.18% 104 83234 0.18% 105 88058 0.19% 106 90638 0.20% 107 93189 0.21% 108 95748 0.21% 109 98499 0.22% 110 100853 0.22% 111 102984 0.23% 112 106015 0.23% 113 109537 0.24% 114 114579 0.25% 115 115959 0.26% 116 119296 0.26% 117 122128 0.27% 118 124446 0.27% 119 125428 0.28% 120 126843 0.28% 121 129142 0.29% 122 131951 0.29% 123 134368 0.30% 124 137665 0.30% 125 140010 0.31% 126 142627 0.31% 127 145238 0.32% 128 145463 0.32% 129 146757 0.32% 130 148935 0.33% 131 149987 0.33% 132 152687 0.34% 133 153694 0.34% 134 158267 0.35% 135 160453 0.35% 136 161351 0.36% 137 164012 0.36% 138 162755 0.36% 139 166011 0.37% 140 168423 0.37% 141 170046 0.38% 142 172062 0.38% 143 174146 0.38% 144 174723 0.39% 145 175897 0.39% 146 182935 0.40% 147 362849 0.80% 148 172407 0.38% 149 170957 0.38% 150 37493444 82.77% 45297063 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=2.54 fanout-score-rank=45 prefix-density=0.26 prefix-fanout=2.3 sequence=TAGGGATAACCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=47 fanout-score=197.59 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=6.0 sequence=CAAAATCTTTGATCTTTGATTCCACTTGTCAAGCAAAAGAAAGGTACTGTTCATGCAGAACACAGAAATACTAACGTCACACCAATTTCACGGATCCATTACACAAAGCAAACACCACAATTATCGAAAAGTTTCCAAGACACTCAACATCTGATAACAGTTACGCCACCAACATT criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=3.64 fanout-score-rank=32 prefix-density=0.28 prefix-fanout=2.2 sequence=AGAGAGAGAGAAGCTGTCGTAATCCACCTAGTATTATCATGTTCCGCAATGACTGGTTGTTGCCCAAAGTTTGGGTCTGAACTTCGGTATGCTGCTGTCCCAGCTGCTTTGGTTGTGTGCCCATATACTATGTAATCGTCCTGTATCAGCCTGTTGTGCTACAATATTTTAATTCTGTGTGGATTTGCTGCTTGTGTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=216.49 fanout-score-rank=1 prefix-density=0.41 prefix-fanout=9.5 sequence=GACAAGGAGGGGAATGCTGCTGGCAATGGCTTTCTCCTGTTGGATTCAGAATTTAATGTCAAAGGACGATGGGAAAAGCCAGGCCACAGCCCCTTGTTTGGCTATGATTTCTGGTACCAGCCTCGTCACAAAACGATGATTAGTTCATCGTGGGGAGCCCCTGCGGCGTTCAGGACAGGTTTTGATCTTCAGCATGTCCAGGATGGTCTATATGGAAGGCACCTGCATGTATACGACTGGCCTGGTGGTGAGCTCAAGCAGACACTGGATTTAGGCAGTACAGGTCTTCTTCCACTGGAGGTGAGATTTTTACATGACCCATCAAAGGACACTGGCTATGTTGGCTGTGCTTTAACTAGCAACATGGTGAGATTCTTCAAAACTGCAGATGGATCATGGAGCCATGAGGTAGCTATATCCATAGAACCATTGAAGGTGC ERR5262786 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:41:07 Started mapping on | Dec 06 11:41:07 Finished on | Dec 06 11:43:59 Mapping speed, Million of reads per hour | 948.08 Number of input reads | 45297063 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 43811689 Uniquely mapped reads % | 96.72% Average mapped length | 290.04 Number of splices: Total | 38832701 Number of splices: Annotated (sjdb) | 35974938 Number of splices: GT/AG | 38245007 Number of splices: GC/AG | 496326 Number of splices: AT/AC | 22333 Number of splices: Non-canonical | 69035 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.10 Insertion rate per base | 0.01% Insertion average length | 2.46 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 502162 % of reads mapped to multiple loci | 1.11% Number of reads mapped to too many loci | 1617 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.16% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 983212 983212 983212 N_multimapping 502162 502162 502162 N_noFeature 1608280 42580481 2002186 N_ambiguous 975730 5581 139101 UnstrandedReadsAssigned:41227679 PositiveStrandReadsAssigned:1225627 NegativeStrandReadsAssigned:41670402 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262786 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262786-trimmed-pair1.fastq ERR5262786-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 45,297,063 reads, 42,142,993 reads pseudoaligned [quant] estimated average fragment length: 267.138 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,260 rounds 52973 ERR5262786.ke.tsv 35125 ERR5262786.se.tsv 88098 total ==> ERR5262786.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 670.265 0 0 PNS24247 1044 777.862 276.126 11.6801 PNS24249 1928 1661.86 663.539 13.1375 PNS24246 1044 777.862 276.126 11.6801 PNS24248 1044 777.862 276.126 11.6801 PNS24244 1471 1204.86 367.082 10.0246 PNS24243 293 100.276 8 2.62503 KQK14069 1603 1336.86 82497.6 2030.46 KQK14071 474 234.5 232.975 32.6893 ==> ERR5262786.se.tsv <== BRADI_1g14170v3 82867 BRADI_1g53295v3 521 BRADI_1g59795v3 819 BRADI_1g07683v3 0 BRADI_1g00485v3 5 BRADI_1g20270v3 1263 BRADI_1g74790v3 3379 BRADI_1g09890v3 0 BRADI_1g77505v3 534 BRADI_1g48960v3 0 ERR5262786 completed mapping pipeline successfully