Starting /dee2/code/volunteer_pipeline.sh ERR5262787 current disk space = 1551500853248 free memory = 1604776084 ERR5262787 SRAfilesize be232e488ce08fbbafe8e3ac36fa071a ERR5262787.sra ERR5262787.sra file validated ERR5262787 is paired end ERR5262787 is conventional basespace ERR5262787 read1 length is 65-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262787_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 65-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.669 37.0 37.0 37.0 37.0 37.0 2 36.4955 37.0 37.0 37.0 37.0 37.0 3 36.5945 37.0 37.0 37.0 37.0 37.0 4 36.609 37.0 37.0 37.0 37.0 37.0 5 36.7415 37.0 37.0 37.0 37.0 37.0 6 36.6835 37.0 37.0 37.0 37.0 37.0 7 36.6585 37.0 37.0 37.0 37.0 37.0 8 36.6775 37.0 37.0 37.0 37.0 37.0 9 36.718 37.0 37.0 37.0 37.0 37.0 10-14 36.6275 37.0 37.0 37.0 37.0 37.0 15-19 36.5398 37.0 37.0 37.0 37.0 37.0 20-24 36.4753 37.0 37.0 37.0 37.0 37.0 25-29 36.446999999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.395199999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.2895 37.0 37.0 37.0 37.0 37.0 40-44 36.278 37.0 37.0 37.0 37.0 37.0 45-49 36.2428 37.0 37.0 37.0 37.0 37.0 50-54 36.122699999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.2213 37.0 37.0 37.0 37.0 37.0 60-64 36.122499999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.843265866466616 37.0 37.0 37.0 37.0 37.0 70-74 35.83480870217554 37.0 37.0 37.0 37.0 37.0 75-79 35.816347560561454 37.0 37.0 37.0 37.0 37.0 80-84 35.704961588333795 37.0 37.0 37.0 37.0 37.0 85-89 35.80181307401409 37.0 37.0 37.0 37.0 37.0 90-94 35.68378306133287 37.0 37.0 37.0 37.0 37.0 95-99 35.6215620109059 37.0 37.0 37.0 37.0 37.0 100-104 35.73397004261369 37.0 37.0 37.0 37.0 37.0 105-109 35.71942820725271 37.0 37.0 37.0 37.0 37.0 110-114 35.58107802195771 37.0 37.0 37.0 37.0 37.0 115-119 35.623510071874215 37.0 37.0 37.0 37.0 37.0 120-124 35.65760563169511 37.0 37.0 37.0 37.0 37.0 125-129 35.685654047359435 37.0 37.0 37.0 37.0 37.0 130-134 35.63738040745265 37.0 37.0 37.0 37.0 37.0 135-139 35.39729583751595 37.0 37.0 37.0 37.0 37.0 140-144 35.42677321160063 37.0 37.0 37.0 37.0 37.0 145-149 35.39983408882954 37.0 37.0 37.0 34.6 37.0 150 35.47432905484247 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 3.0 23 1.0 24 3.0 25 6.0 26 8.0 27 8.0 28 9.0 29 22.0 30 20.0 31 41.0 32 77.0 33 123.0 34 282.0 35 505.0 36 2591.0 37 299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.275 11.4 9.45 34.875 2 19.85992996498249 9.67983991995998 31.265632816408207 39.19459729864933 3 15.475 15.775 25.4 43.35 4 14.799999999999999 28.475 26.825 29.9 5 15.375 32.1 26.575 25.95 6 17.424999999999997 40.849999999999994 20.8 20.925 7 12.525 27.224999999999998 38.45 21.8 8 17.775 26.35 26.224999999999998 29.65 9 15.35 25.45 30.975 28.225 10-14 16.445 28.77 28.005000000000003 26.779999999999998 15-19 18.54 26.525 26.86 28.075 20-24 18.505 27.215 26.435 27.845 25-29 18.715 31.259999999999998 26.484999999999996 23.54 30-34 18.475 26.38 26.840000000000003 28.305000000000003 35-39 19.900000000000002 28.244999999999997 25.195 26.66 40-44 17.24 29.054999999999996 26.85 26.855 45-49 15.584999999999999 32.81 28.660000000000004 22.945 50-54 18.175 30.005 29.195 22.625 55-59 18.65 33.42 25.585 22.345000000000002 60-64 15.8 31.915 29.304999999999996 22.98 65-69 17.623524704940987 32.53150630126026 27.805561112222442 22.039407881576313 70-74 20.25006251562891 31.337834458614655 26.051512878219558 22.360590147536886 75-79 19.57772552158903 36.17351278330915 23.58032721268825 20.66843448241357 80-84 19.174854622017246 29.98796871866854 29.236013635452174 21.601163023862043 85-89 18.612240799317167 32.223728473163625 28.734247125571123 20.429783601948085 90-94 21.44222065774917 27.853766468872575 30.805591873679976 19.89842099969828 95-99 18.555505119281786 33.060977454985625 26.559741766278304 21.82377565945428 100-104 17.318492284341005 30.83227928155831 28.727548697192006 23.121679736908675 105-109 18.849872773536898 29.928753180661577 27.2824427480916 23.938931297709924 110-114 19.07221767594108 31.873977086743043 24.83633387888707 24.217471358428806 115-119 20.17036654620547 30.01548786783686 25.322663913267938 24.491481672689726 120-124 20.314048724503103 29.412071573895354 25.003912567165738 25.269967134435806 125-129 22.124642894931753 28.64776214157232 24.35192043170035 24.87567453179558 130-134 23.746715993780494 29.070827301485174 24.35258163101174 22.82987507372259 135-139 21.68969644217169 28.060058753128057 25.688173212925687 24.56207159177456 140-144 23.97039180765806 26.43032056990205 23.491763134461262 26.10752448797863 145-149 23.09949933820567 26.201300569718594 24.072049260516774 26.62715083155896 150 26.77946324387398 26.546091015169193 23.5414235705951 23.133022170361727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.5 19 0.5 20 0.5 21 1.0 22 1.0 23 2.0 24 2.0 25 1.5 26 4.0 27 4.0 28 1.5 29 2.5 30 14.5 31 17.0 32 17.0 33 26.0 34 57.5 35 96.5 36 138.5 37 201.5 38 308.0 39 326.0 40 277.5 41 264.0 42 221.5 43 218.0 44 368.0 45 367.0 46 204.0 47 197.0 48 148.5 49 52.0 50 19.5 51 9.0 52 5.0 53 15.5 54 23.0 55 32.0 56 38.0 57 35.5 58 79.0 59 86.5 60 60.5 61 38.0 62 6.0 63 6.5 64 4.5 65 1.0 66 0.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 1.0 75 1.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 64-65 1.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.0 78-79 5.0 80-81 0.0 82-83 5.0 84-85 0.0 86-87 4.0 88-89 1.0 90-91 3.0 92-93 5.0 94-95 8.0 96-97 2.0 98-99 3.0 100-101 5.0 102-103 13.0 104-105 10.0 106-107 2.0 108-109 10.0 110-111 8.0 112-113 16.0 114-115 17.0 116-117 11.0 118-119 16.0 120-121 18.0 122-123 20.0 124-125 16.0 126-127 36.0 128-129 18.0 130-131 11.0 132-133 21.0 134-135 20.0 136-137 25.0 138-139 34.0 140-141 34.0 142-143 47.0 144-145 72.0 146-147 25.0 148-149 27.0 150-151 3428.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.874999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 45.21739130434783 11.700000000000001 2 18.647342995169083 9.65 3 8.88888888888889 6.9 4 7.536231884057972 7.8 5 4.251207729468599 5.5 6 2.1256038647342996 3.3000000000000003 7 1.932367149758454 3.5000000000000004 8 2.2222222222222223 4.6 9 0.966183574879227 2.25 >10 7.632850241545894 33.5 >50 0.3864734299516908 6.0249999999999995 >100 0.1932367149758454 5.2749999999999995 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGG 108 2.7 No Hit GCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACAC 103 2.5749999999999997 No Hit GGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAG 62 1.55 No Hit GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 61 1.525 No Hit GGCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCC 60 1.5 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 58 1.4500000000000002 No Hit CCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 48 1.2 No Hit GTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAAT 47 1.175 No Hit GTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 46 1.15 No Hit CTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGA 44 1.0999999999999999 No Hit TAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAG 42 1.05 No Hit AGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCA 39 0.975 No Hit GCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATAC 33 0.8250000000000001 No Hit GCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATAT 30 0.75 No Hit GGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAAT 23 0.575 No Hit GGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAA 23 0.575 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 23 0.575 No Hit GCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCT 22 0.5499999999999999 No Hit GGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCT 21 0.525 No Hit CAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTC 21 0.525 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 21 0.525 No Hit CTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACT 20 0.5 No Hit CTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCT 20 0.5 No Hit CCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGC 19 0.475 No Hit CTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATG 19 0.475 No Hit GGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCT 19 0.475 No Hit CTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGA 18 0.44999999999999996 No Hit GTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCG 18 0.44999999999999996 No Hit GCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAGA 17 0.42500000000000004 No Hit CCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATA 17 0.42500000000000004 No Hit CGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTC 17 0.42500000000000004 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 17 0.42500000000000004 No Hit CCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAAC 17 0.42500000000000004 No Hit GCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAA 16 0.4 No Hit GCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAAT 16 0.4 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 16 0.4 No Hit GTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAA 16 0.4 No Hit CCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGA 16 0.4 No Hit ATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAGGC 16 0.4 No Hit GGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCT 15 0.375 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 15 0.375 No Hit ACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTT 15 0.375 No Hit ACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACA 15 0.375 No Hit GCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCC 15 0.375 No Hit CTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAG 15 0.375 No Hit CTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAA 14 0.35000000000000003 No Hit ATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAG 14 0.35000000000000003 No Hit GCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAA 14 0.35000000000000003 No Hit CTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAA 14 0.35000000000000003 No Hit GCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCA 13 0.325 No Hit CTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGA 13 0.325 No Hit GCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAA 13 0.325 No Hit CGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTT 13 0.325 No Hit CCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACAT 13 0.325 No Hit GCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGC 13 0.325 No Hit CCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACA 12 0.3 No Hit CACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTG 12 0.3 No Hit GATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTT 12 0.3 No Hit GGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCC 12 0.3 No Hit ATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCT 12 0.3 No Hit CCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGAT 12 0.3 No Hit ATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGG 11 0.27499999999999997 No Hit CACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATAA 11 0.27499999999999997 No Hit GGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCA 11 0.27499999999999997 No Hit TCTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGC 11 0.27499999999999997 No Hit CTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTT 11 0.27499999999999997 No Hit GTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAAGACTCCT 11 0.27499999999999997 No Hit CTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGA 11 0.27499999999999997 No Hit CCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGGAACCTGAGTTGC 10 0.25 No Hit GTCAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAAT 10 0.25 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 10 0.25 No Hit GCATATCGGTCCCCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCT 10 0.25 No Hit GGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 10 0.25 No Hit CCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTT 10 0.25 No Hit GCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCA 10 0.25 No Hit GCCTGAGTGGTTTTGCAGAATGTGATCAACTATGCTGGTGATGTCCCTTT 10 0.25 No Hit GTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATA 10 0.25 No Hit ATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATA 10 0.25 No Hit GTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCG 10 0.25 No Hit CTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGAT 10 0.25 No Hit CTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGATCCAA 10 0.25 No Hit CCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACAT 10 0.25 No Hit CCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 10 0.25 No Hit ATCCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAA 10 0.25 No Hit GCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTT 10 0.25 No Hit CATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCA 9 0.22499999999999998 No Hit CTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGACCT 9 0.22499999999999998 No Hit CTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACA 9 0.22499999999999998 No Hit CTAACAGTCTGAAACATTGCATGTCAAGAATCTAGGCTAATAGAATTAGG 9 0.22499999999999998 No Hit GATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAGG 9 0.22499999999999998 No Hit CTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACC 9 0.22499999999999998 No Hit CCTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCG 9 0.22499999999999998 No Hit CGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGC 9 0.22499999999999998 No Hit GCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGT 9 0.22499999999999998 No Hit GAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAGGCAT 9 0.22499999999999998 No Hit GTCGAAAACATGTACAATTCTGAGGTTTATCAGGTCAACCTAACAGTCTG 8 0.2 No Hit GCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCA 8 0.2 No Hit CCCCTCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 8 0.2 No Hit CACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCA 8 0.2 No Hit AGCATGTCAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACA 8 0.2 No Hit TTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTA 8 0.2 No Hit CCGCAGTTGGCCAAACGCATTCAGACAAATGCATATCGGTCCCCTTGCTC 8 0.2 No Hit GGGGTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTCCA 8 0.2 No Hit CGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAG 8 0.2 No Hit GGTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTCCACA 8 0.2 No Hit CACATGAGCTACCATTGTAGAAATCCTCAAACAGGAGGACGGAATGGCTC 8 0.2 No Hit TGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATC 8 0.2 No Hit CAGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACT 8 0.2 No Hit GTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAA 8 0.2 No Hit GTCCAGCTTCTCCAGTTCGCCCATCCTTTCTATGATTGCCTTTTTGCTTG 8 0.2 No Hit CTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGCCAAAA 8 0.2 No Hit CCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACAC 8 0.2 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 8 0.2 No Hit GCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAA 8 0.2 No Hit TCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCG 8 0.2 No Hit GCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCG 8 0.2 No Hit CCCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCA 8 0.2 No Hit GTCCCCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCTGACAAATC 8 0.2 No Hit CTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 7 0.17500000000000002 No Hit TTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGAT 7 0.17500000000000002 No Hit ACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCC 7 0.17500000000000002 No Hit CGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAG 7 0.17500000000000002 No Hit GGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTA 7 0.17500000000000002 No Hit ATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAAT 7 0.17500000000000002 No Hit CGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATA 7 0.17500000000000002 No Hit GCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTC 7 0.17500000000000002 No Hit ATTTGTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGC 7 0.17500000000000002 No Hit GTCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCG 7 0.17500000000000002 No Hit TCCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGGAACCTGAGTTG 7 0.17500000000000002 No Hit CACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAA 7 0.17500000000000002 No Hit AGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAG 7 0.17500000000000002 No Hit GAATGTTATTGCCATCAGTAGATTCGCCAAAAAAGTGCTCCCGGTTCAGA 7 0.17500000000000002 No Hit GTCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAG 7 0.17500000000000002 No Hit GAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTT 7 0.17500000000000002 No Hit CCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 7 0.17500000000000002 No Hit ACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTT 7 0.17500000000000002 No Hit CCACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACATTCC 7 0.17500000000000002 No Hit CCCCCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCTGACAAATCT 7 0.17500000000000002 No Hit CAGCTGCCCTACACGAACCCTTGACACATCTGGTGGGCATAGAGCGCAGA 6 0.15 No Hit TCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTT 6 0.15 No Hit CTGTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAA 6 0.15 No Hit CGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGA 6 0.15 No Hit TGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAG 6 0.15 No Hit CAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGG 6 0.15 No Hit CCTCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 6 0.15 No Hit GTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCA 6 0.15 No Hit CCTTCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACAT 6 0.15 No Hit CTTCATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTTTAACAAA 6 0.15 No Hit ATCTAATTTATATTTGTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGC 6 0.15 No Hit GACCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCACAGAG 6 0.15 No Hit CGCTTCCAATAAGATTTTTTGAACCTTGTGCGATACCATTTAATAGCTCA 6 0.15 No Hit ACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGA 6 0.15 No Hit ATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCAT 6 0.15 No Hit AGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 6 0.15 No Hit TTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAAGA 6 0.15 No Hit ATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGA 6 0.15 No Hit CCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTG 6 0.15 No Hit GGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAATTCGGAATGTTAT 6 0.15 No Hit CTCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 6 0.15 No Hit GCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTT 6 0.15 No Hit CCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAAC 5 0.125 No Hit GCCACATAAGATTTTTCCAGATATGAGATAATGAAAACAACTAACAACGC 5 0.125 No Hit GACCCTTATAGGCAGTTTTACTAAAGTGAGAAGTGGCACTACTGACTGCA 5 0.125 No Hit TTTTTTTTTTGAACAAGCTATCTTTAGCTAGGCCTGTGATTTGTCTATAG 5 0.125 No Hit CCTTCTCCAAGTTTTCCTTTCCCAGAAAGTAATCCCAAACCAAATGCTAC 5 0.125 No Hit ATAACTATTGAGGCCCTATACCTGTTATGAAAAAGAAAGCCAGTGCAAGC 5 0.125 No Hit GGTCAACCTAACAGTCTGAAACATTGCATGTCAAGAATCTAGGCTAATAG 5 0.125 No Hit GGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCC 5 0.125 No Hit GGAATGGCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTT 5 0.125 No Hit GGAATGATAAACAGAAGGGGAGCTGAGAAAAAACAAAAACGTAAGTGGAT 5 0.125 No Hit CAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAG 5 0.125 No Hit TCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAA 5 0.125 No Hit CTTGAGAATCTTGTGACGGTACTCGAGGCTCCTCTTGAGACCATGACGAA 5 0.125 No Hit AGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGC 5 0.125 No Hit GATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAA 5 0.125 No Hit CATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGG 5 0.125 No Hit ACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCA 5 0.125 No Hit ACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGA 5 0.125 No Hit CCCTCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 5 0.125 No Hit ATAGCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATA 5 0.125 No Hit CCAGTGTATATGTGTTCTATCTTCCTTGCAGCAGCATCCTTCACAGAGGG 5 0.125 No Hit CATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAA 5 0.125 No Hit GGCCTCACTGGAAATCACGCATCGACACGACAGGGCACGCACGCCCGGCG 5 0.125 No Hit CGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATC 5 0.125 No Hit GAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAA 5 0.125 No Hit ACCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAAT 5 0.125 No Hit CCCCTTGCTCTTGACTCTCATTATCTCGTGTTTCCGATCTGACAAATCTT 5 0.125 No Hit GGGTACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTC 5 0.125 No Hit GCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGG 5 0.125 No Hit CCCTGATGTGTTGAGTCAACAACTCCTCCTTGAGCCACCTCATCTAACAG 5 0.125 No Hit GCCCCTTCCATCTCCTCAGAGATATCATCGTCATACTGAAGAATAACAGA 5 0.125 No Hit GGTCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTT 5 0.125 No Hit TTTTTTTTTTTAAACGTTTGAGCCACAAATTTATTCAGATACCAGTATAT 5 0.125 No Hit GTGACGGTACTCGAGGCTCCTCTTGAGACCATGACGAAAAAACTGGGGGT 5 0.125 No Hit GCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAA 5 0.125 No Hit CAAACGTATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTG 5 0.125 No Hit CTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCT 5 0.125 No Hit TCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAA 5 0.125 No Hit CCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGC 5 0.125 No Hit TGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAG 5 0.125 No Hit GCCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCA 5 0.125 No Hit GAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGT 5 0.125 No Hit CATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCA 5 0.125 No Hit CTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCCCATCCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTAAA 10 0.0073835873 141.275 1 CTCATAA 65 0.009071632 13.04077 20-24 >>END_MODULE ERR5262787 read2 length is 65-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262787_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 65-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.114 37.0 37.0 37.0 37.0 37.0 2 35.759 37.0 37.0 37.0 37.0 37.0 3 35.9025 37.0 37.0 37.0 37.0 37.0 4 36.1005 37.0 37.0 37.0 37.0 37.0 5 36.2525 37.0 37.0 37.0 37.0 37.0 6 36.0585 37.0 37.0 37.0 37.0 37.0 7 35.9835 37.0 37.0 37.0 37.0 37.0 8 36.1235 37.0 37.0 37.0 37.0 37.0 9 36.1495 37.0 37.0 37.0 37.0 37.0 10-14 36.181400000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.17810000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.1256 37.0 37.0 37.0 37.0 37.0 25-29 36.132400000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.0157 37.0 37.0 37.0 37.0 37.0 35-39 35.9995 37.0 37.0 37.0 37.0 37.0 40-44 36.04690000000001 37.0 37.0 37.0 37.0 37.0 45-49 35.9569 37.0 37.0 37.0 37.0 37.0 50-54 35.9393 37.0 37.0 37.0 37.0 37.0 55-59 35.970000000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.8988 37.0 37.0 37.0 37.0 37.0 65-69 35.92568702175544 37.0 37.0 37.0 37.0 37.0 70-74 35.89222305576394 37.0 37.0 37.0 37.0 37.0 75-79 35.80832711220627 37.0 37.0 37.0 37.0 37.0 80-84 35.75484619215503 37.0 37.0 37.0 37.0 37.0 85-89 35.72930592916435 37.0 37.0 37.0 37.0 37.0 90-94 35.685943885994305 37.0 37.0 37.0 37.0 37.0 95-99 35.62498022247935 37.0 37.0 37.0 37.0 37.0 100-104 35.56334407792255 37.0 37.0 37.0 37.0 37.0 105-109 35.50385272531775 37.0 37.0 37.0 37.0 37.0 110-114 35.47439116749333 37.0 37.0 37.0 37.0 37.0 115-119 35.41791537548024 37.0 37.0 37.0 37.0 37.0 120-124 35.374375341655735 37.0 37.0 37.0 37.0 37.0 125-129 35.327133185545456 37.0 37.0 37.0 34.6 37.0 130-134 35.279023412113304 37.0 37.0 37.0 32.2 37.0 135-139 35.25116810144491 37.0 37.0 37.0 27.4 37.0 140-144 35.13698323992191 37.0 37.0 37.0 27.4 37.0 145-149 35.16234866830449 37.0 37.0 37.0 25.0 37.0 150 35.11175785797439 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 2.0 13 3.0 14 5.0 15 1.0 16 1.0 17 0.0 18 2.0 19 1.0 20 1.0 21 5.0 22 11.0 23 9.0 24 7.0 25 8.0 26 7.0 27 11.0 28 11.0 29 17.0 30 23.0 31 46.0 32 59.0 33 110.0 34 231.0 35 621.0 36 2563.0 37 242.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.425000000000004 23.325000000000003 8.85 24.4 2 29.425 24.675 26.3 19.6 3 22.6 24.625 30.925000000000004 21.85 4 27.175 27.625 23.625 21.575 5 30.175 32.324999999999996 19.85 17.65 6 25.525 34.1 19.75 20.625 7 21.6 21.325 35.05 22.025 8 24.375 23.974999999999998 24.75 26.900000000000002 9 23.1 22.0 28.225 26.674999999999997 10-14 24.89 26.135 25.345000000000002 23.630000000000003 15-19 24.959999999999997 24.815 26.56 23.665 20-24 26.634999999999998 25.345000000000002 25.94 22.08 25-29 25.305 25.275 26.27 23.150000000000002 30-34 26.52 25.835 26.25 21.395 35-39 25.1 26.07 26.834999999999997 21.995 40-44 26.44 24.715 26.255 22.59 45-49 25.369999999999997 25.185000000000002 27.060000000000002 22.384999999999998 50-54 25.224999999999998 25.85 26.91 22.015 55-59 24.65 26.939999999999998 26.88 21.529999999999998 60-64 24.69 24.654999999999998 28.705000000000002 21.95 65-69 24.5499099819964 26.210242048409683 27.130426085217042 22.109421884376875 70-74 25.096274068517125 24.996249062265566 27.956989247311824 21.950487621905477 75-79 25.956871966778404 25.871816680842546 26.712363035973386 21.458948316405664 80-84 24.699217966713455 25.97754160818127 28.539201925005013 20.78403850010026 85-89 24.255660993121452 26.07320379575237 27.66982979364362 22.001305417482552 90-94 24.343759428743837 26.988836367293572 27.315699487076333 21.351704716886253 95-99 25.26352953043829 24.85499571291673 28.345185857668838 21.536288898976146 100-104 25.701998482165443 25.605868960283328 26.88590943587149 21.80622312167974 105-109 25.674300254452927 25.54198473282443 28.458015267175576 20.325699745547073 110-114 23.69847601513757 26.55722614298865 28.531246803723025 21.213051038150763 115-119 24.509196114899773 26.136598470758422 27.94998966728663 21.404215747055176 120-124 24.34138452710105 26.459387552819656 27.914862538473578 21.284365381605717 125-129 25.917892286530524 26.256480795682997 27.34631255951751 20.479314358268965 130-134 25.227930923522475 26.997747506167542 26.9870213450606 20.787300225249382 135-139 24.92518635399097 27.253931117035744 27.39539692039828 20.425485608575002 140-144 26.346336291087674 26.190077571293042 28.176795580110497 19.28679055750879 145-149 25.091996320147192 27.282658693652255 27.20791168353266 20.41743330266789 150 26.047729918509894 25.20372526193248 29.59837019790454 19.150174621653086 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 1.0 17 1.0 18 0.5 19 1.5 20 1.0 21 0.5 22 1.0 23 1.0 24 1.0 25 1.0 26 2.0 27 2.0 28 0.5 29 1.5 30 2.0 31 11.5 32 21.5 33 21.5 34 22.0 35 30.0 36 53.0 37 87.5 38 117.5 39 122.5 40 128.5 41 189.5 42 206.5 43 214.0 44 236.5 45 212.0 46 208.0 47 198.0 48 177.5 49 181.0 50 171.0 51 129.0 52 142.5 53 183.5 54 138.5 55 90.5 56 96.5 57 87.5 58 90.0 59 95.0 60 67.0 61 41.5 62 35.5 63 29.0 64 21.5 65 14.0 66 7.0 67 8.5 68 24.0 69 23.0 70 7.5 71 11.0 72 9.5 73 4.0 74 3.5 75 2.5 76 4.5 77 4.0 78 2.0 79 2.0 80 0.5 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 64-65 1.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.0 78-79 5.0 80-81 0.0 82-83 5.0 84-85 0.0 86-87 4.0 88-89 1.0 90-91 3.0 92-93 5.0 94-95 8.0 96-97 2.0 98-99 3.0 100-101 5.0 102-103 13.0 104-105 10.0 106-107 2.0 108-109 10.0 110-111 8.0 112-113 17.0 114-115 18.0 116-117 13.0 118-119 13.0 120-121 16.0 122-123 21.0 124-125 16.0 126-127 36.0 128-129 18.0 130-131 12.0 132-133 22.0 134-135 19.0 136-137 24.0 138-139 42.0 140-141 34.0 142-143 53.0 144-145 50.0 146-147 25.0 148-149 27.0 150-151 3436.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.5 #Duplication Level Percentage of deduplicated Percentage of total 1 52.472527472527474 23.875 2 20.934065934065934 19.05 3 10.824175824175825 14.774999999999999 4 6.4835164835164845 11.799999999999999 5 2.5274725274725274 5.75 6 2.307692307692308 6.3 7 1.5384615384615385 4.9 8 0.9340659340659341 3.4000000000000004 9 0.7692307692307693 3.15 >10 1.208791208791209 7.000000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTT 19 0.475 No Hit GGAGAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGGTCCACCG 18 0.44999999999999996 No Hit CAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTGAC 17 0.42500000000000004 No Hit GAAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAA 16 0.4 No Hit GATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGAAGTCTTTTT 15 0.375 No Hit GTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTG 14 0.35000000000000003 No Hit GTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTTCAGTGACTTC 14 0.35000000000000003 No Hit GTTTGCAGGTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCG 14 0.35000000000000003 No Hit TGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTT 13 0.325 No Hit CCCAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTG 13 0.325 No Hit CACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCGCCCTGTTGAGGTTT 12 0.3 No Hit GTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAAC 12 0.3 No Hit GCCAAAGACAAACTAGAGCACCCAAGGTTATTGAAGTGTTGCGGGGGAGA 11 0.27499999999999997 No Hit CAAGAATGTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTTCAG 11 0.27499999999999997 No Hit GACCAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCC 11 0.27499999999999997 No Hit GTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTTG 10 0.25 No Hit GGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTG 10 0.25 No Hit GTTTTGACCGGGTTGTAGAGGCTGCACTAGGCATCGATTTGCGTCATCGA 10 0.25 No Hit CTCAATTGTTTCTTTGGCGTGAGTCCGTTCTCTCCCCCATCCCCTGCACA 10 0.25 No Hit GGCAAGGGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAAGGAGA 10 0.25 No Hit GGGACAAGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACA 10 0.25 No Hit GTCTGGATCATCGCAAATGGATGGCACAATGGAGACCACCCTGCTTCACC 10 0.25 No Hit GAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTATTGAAGTG 9 0.22499999999999998 No Hit AGAAGGATGACCAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAG 9 0.22499999999999998 No Hit TCGCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCATGAAGAACTCCATT 9 0.22499999999999998 No Hit CCGTACTCCCGGGATCTGCAGTTTTTTGCCCTTCGTTTTACCCTCTCTCC 9 0.22499999999999998 No Hit GCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGC 9 0.22499999999999998 No Hit GTTCTCTCCCCCATCCCCTGCACACTATTTTTTCCTATGACTGGAATATG 9 0.22499999999999998 No Hit AGAGGCTGCACTAGGCATCGATTTGCGTCATCGAGGTTTGCAGGTCCGGT 9 0.22499999999999998 No Hit CTTCGTGGTGATCAAGGCCGACACGCCGTGGCATTATAGCGAGGATGGGG 9 0.22499999999999998 No Hit GGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTT 9 0.22499999999999998 No Hit GCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAG 9 0.22499999999999998 No Hit CTTCTACAACTACGACATGGAGAAGAGTGAATTTGTGCAGCTCTTCATCA 9 0.22499999999999998 No Hit GGGGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCAT 9 0.22499999999999998 No Hit AGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGT 9 0.22499999999999998 No Hit TGGAGCAGGAGAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGG 9 0.22499999999999998 No Hit CAGGTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTAT 8 0.2 No Hit GCAGGTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGC 8 0.2 No Hit AGAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTATTGA 8 0.2 No Hit GTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCGCCC 8 0.2 No Hit CAAGAATAAAGCTTTATTAGCAGGTGGTGGTCTGGATCATCGCAAATGGA 8 0.2 No Hit CCCGGGATCTGCAGTTTTTTGCCCTTCGTTTTACCCTCTCTCCGTGTTCG 8 0.2 No Hit GCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGT 8 0.2 No Hit GGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTT 8 0.2 No Hit ATTGAGGGTTCATACTCAAGCAGGAGGAAATAGTCATCAGCGCTTCTATA 8 0.2 No Hit CAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATTGACGCTG 8 0.2 No Hit CAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTC 8 0.2 No Hit TTATGATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCCGTGTTC 8 0.2 No Hit GTCTTCGGAAGCACAACTCGCAGCAGATTCGTCACCTTGTGCAGACACTC 8 0.2 No Hit GTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCGCCCTGTTG 8 0.2 No Hit GACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTT 8 0.2 No Hit CATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGACCTTAAAA 8 0.2 No Hit GAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGCC 8 0.2 No Hit ATGGATTCCAGGAGTCTTCAGTGACTTCTGCCATGCCAAAAAAAAGATGA 7 0.17500000000000002 No Hit GGGGACAAAAGGAGACGTTTCTGTACTGAAACCCACTCTTATGATTTCAG 7 0.17500000000000002 No Hit GTGAGCCAGTGAGGTAGCCTGGTGCCCCGCGCTAAACTAAAAAGATCTGT 7 0.17500000000000002 No Hit CCTGCACACTATTTTTTCCTATGACTGGAATATGAAAACTTCTTTGTACT 7 0.17500000000000002 No Hit ATTTGCAACCCTGAGACTGAATGCAATGGAAAGTTGTTCTGGGCCTGTGT 7 0.17500000000000002 No Hit GAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTG 7 0.17500000000000002 No Hit ATAAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAA 7 0.17500000000000002 No Hit CCGGGTTGTAGAGGCTGCACTAGGCATCGATTTGCGTCATCGAGGTTTGC 7 0.17500000000000002 No Hit GAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAAAGA 7 0.17500000000000002 No Hit GCAAAACATCTCATACAGATCCAAGATTACCATCAAGCAGTGATACCGCA 7 0.17500000000000002 No Hit GACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGACCTTAAAAACTA 7 0.17500000000000002 No Hit CAAGTTTACACAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCTTCCTGG 7 0.17500000000000002 No Hit GTTTACACAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCA 7 0.17500000000000002 No Hit GGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGG 7 0.17500000000000002 No Hit CACTGACAATGAGTTGGCATACTTGGAATGCATCCACTTGTTTGTTGAAA 7 0.17500000000000002 No Hit ACAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCATGAAGA 7 0.17500000000000002 No Hit ACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGCCGTGGCAT 7 0.17500000000000002 No Hit GTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTCG 7 0.17500000000000002 No Hit GTTGACATCTCTGGTGTTAAGGTGGAGAAGTTTGATGACAAGTACTTTGC 7 0.17500000000000002 No Hit GGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAG 7 0.17500000000000002 No Hit AATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATTGACGCTGAGTT 7 0.17500000000000002 No Hit GTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAATTTTGGA 7 0.17500000000000002 No Hit GCCCGGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTA 7 0.17500000000000002 No Hit GGTGGCCACAACTGCTGCCGTCAGGACAATCATTCCTAAACTTGGCACGG 7 0.17500000000000002 No Hit GTTTCTGAGTTTTGTGGAAGTCTTTTTGCCTAGCTTGTTTCTGTACTTCT 7 0.17500000000000002 No Hit GATGGATTCCAGGAGTCTTCAGTGACTTCTGCCATGCCAAAAAAAAGATG 7 0.17500000000000002 No Hit AGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAAGGAGACTACCAAGAAT 7 0.17500000000000002 No Hit CCTGGGATCACCTTACATTGCATAGGTCTCTCAATTGTTTCTTTGGCGTG 7 0.17500000000000002 No Hit ATTTCAAGGCCTTTTTGATGAAGAATATCACCCGGACGTATACTCAATAA 6 0.15 No Hit ACAAGTTTCTGAGTTTTGTGGAAGTCTTTTTGCCTAGCTTGTTTCTGTAC 6 0.15 No Hit GGAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCA 6 0.15 No Hit GTCTGATTTAAAAAGCTACCATACCTGTTCCGTTGTGGTCAAATCTTATG 6 0.15 No Hit GCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACA 6 0.15 No Hit GAAGAGCATAAAATGCCTCGACCTGAAGGTTGATGGGCCATGATTGGTGG 6 0.15 No Hit AGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATT 6 0.15 No Hit CTGTGAGCTATCCCAATATTGATGAAATGAGTAAAGAATCTGAGAAGCCT 6 0.15 No Hit GATGGATAGCAAGCTCCAAGCGTAATCCCTGGAAGTTTTGACCGGGTTGT 6 0.15 No Hit GAGCGAATAATCTGTTACGAACTTTTTTTTGGCCTAGCGATTTTAGTAAA 6 0.15 No Hit GCTAAACAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTGT 6 0.15 No Hit GGTTGATGGGCCATGATTGGTGGACTAGATGCAGTTGACATGTTACTGGA 6 0.15 No Hit GGGAGAACTGTTGGTGCTGGAGTCATCGCTAAAGTTATGAGCGAATAATC 6 0.15 No Hit GGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAAGGAGACTACCA 6 0.15 No Hit AGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTGACA 6 0.15 No Hit GGATAGCAAGCTCCAAGCGTAATCCCTGGAAGTTTTGACCGGGTTGTAGA 6 0.15 No Hit AGAAGTTGGTGTCTGTACTAAGTGAAATTTTACCCTGCAGATGGGAGAAA 6 0.15 No Hit AACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGA 6 0.15 No Hit AAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTG 6 0.15 No Hit TTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAAG 6 0.15 No Hit GGAGGGAGAACTGTTGGTGCTGGAGTCATCGCTAAAGTTATGAGCGAATA 6 0.15 No Hit GTCCGTTCTCTCCCCCATCCCCTGCACACTATTTTTTCCTATGACTGGAA 6 0.15 No Hit ATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCA 6 0.15 No Hit TCTATTTCCAATGTCTAGTAGTAGTATATTTGATTACTTGAAATTTGGGA 6 0.15 No Hit GCCCTTCTTGCGCCCGGCTGCGGGTATCCGCTTCGTGATCGATAGGGAGG 6 0.15 No Hit ATCGATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATA 6 0.15 No Hit AGAGCACCCAAGGTTATTGAAGTGTTGCGGGGGAGACGGTCTGATTTAAA 6 0.15 No Hit GTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTGCAATA 6 0.15 No Hit ATGGAGAAGAGTGAATTTGTGCAGCTCTTCATCAAGTTTACACAGAATCT 6 0.15 No Hit AGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTGCAATAGATGGA 6 0.15 No Hit AGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGC 6 0.15 No Hit GGACAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTT 6 0.15 No Hit GACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTC 6 0.15 No Hit TGGTGATCAAGGCCGACACGCCGTGGCATTATAGCGAGGATGGGGTGGAT 6 0.15 No Hit TGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCAATAAATCCCCAT 6 0.15 No Hit GATAGATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTT 6 0.15 No Hit TACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATTGACG 6 0.15 No Hit GTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAGA 6 0.15 No Hit GCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGACCTTAAAAACTATCT 6 0.15 No Hit CACAGGACCATTCAAGATCAATGGAGTGCCAATTCGCCGCGTCAACCAGA 6 0.15 No Hit GATTGGACCCCTGTTTTGGAGCAAGGCAGGCCAGTCTTGTCTCAGTTTCC 6 0.15 No Hit CTCGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGAGGG 6 0.15 No Hit CATAGACTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAACAACATG 5 0.125 No Hit CCCTGCTTCACCCTGGATGATGGATAGCAAGCTCCAAGCGTAATCCCTGG 5 0.125 No Hit CTTGTCCTTATCTTGTACGTGCCCCGGCCCCTTCCATGGACGCACGGATC 5 0.125 No Hit CCTGACTTCAAGAAGGATGACCAGAAGACCATTGACGCTGAGTTGATCAA 5 0.125 No Hit GCCTGACTTCAAGAAGGATGACCAGAAGACCATTGACGCTGAGTTGATCA 5 0.125 No Hit ACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAGACTTT 5 0.125 No Hit AGGGCGAGGTGCACCGGCTGGTGGTCAACCGGGACCCCAAGTTCACCAAC 5 0.125 No Hit AGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGAC 5 0.125 No Hit CAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGA 5 0.125 No Hit TGATGACCTACTTGAGAGTGAGCTTGAACATGGTGTGGAATTGGCTGTTG 5 0.125 No Hit GGACAGAGGTCGGACATCCTCATCCAGGTGCCCAACGGCGCCGTCGGCGG 5 0.125 No Hit GTGTATTTAGTGAAGCTGATCTAGAACCATAGGATCTTGTTTTGATGTGC 5 0.125 No Hit AGGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCG 5 0.125 No Hit CTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACA 5 0.125 No Hit ACTTTGCTAGGGACAAGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTT 5 0.125 No Hit CATTGGTTGATGGTCTTGATGGCTTGGCCGGTGGTGTTGCTGCTTTAGCA 5 0.125 No Hit CACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAA 5 0.125 No Hit GCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATA 5 0.125 No Hit CGAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGC 5 0.125 No Hit GTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTCGTGACAAGACTAG 5 0.125 No Hit GGAGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTAT 5 0.125 No Hit CCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTCATTTTTCAT 5 0.125 No Hit AAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTC 5 0.125 No Hit AGTTGATCAAGGCTATTGAGGCCGTCCCAGACCTTAAAAACTATCTTGGT 5 0.125 No Hit CGGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGT 5 0.125 No Hit GGCATGTTATCTATATTGAGGATGAACCTGTTGAGGCTGACACACTTAAC 5 0.125 No Hit ACGAAACCATAGACTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAA 5 0.125 No Hit AATAAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTCA 5 0.125 No Hit ACTCGAACCAGGGCAAAGATTTGCACTGAGGGAAGGAGGGAGAACTGTTG 5 0.125 No Hit CAAGGCTATTGAGGCCGTCCCAGACCTTAAAAACTATCTTGGTGCCCGGT 5 0.125 No Hit GCTGCTACCATTGGTTTGAAGGGTCTGGGAGGTCTCCTGTTTATCTTCAG 5 0.125 No Hit AGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGG 5 0.125 No Hit AGTTTCCCAAGGGGCTGGGAGAGAGCCTCGGGGGAAGGATCAAGAGAGGC 5 0.125 No Hit ACAATGGAGACCACCCTGCTTCACCCTGGATGATGGATAGCAAGCTCCAA 5 0.125 No Hit CCCAAGGTTATTGAAGTGTTGCGGGGGAGACGGTCTGATTTAAAAAGCTA 5 0.125 No Hit AATATCGATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGC 5 0.125 No Hit GAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTATTGAA 5 0.125 No Hit GATACATGATGGAGGCGCTGAGTTGGAGAACGGCGTGCCTCGTGGTCCTC 5 0.125 No Hit AGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATCC 5 0.125 No Hit TGAGGGTTCATACTCAAGCAGGAGGAAATAGTCATCAGCGCTTCTATATT 5 0.125 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 5 0.125 No Hit CAAGAGAGGCTCGCCCGCGCGCGTGATGAATTGATTAATACGGCGGCCGC 5 0.125 No Hit GTTGATGTCACAGAACAGCGTTCTCCAACCAAGAGAAGTGACAGTTTGGA 5 0.125 No Hit GGATGACCAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCG 5 0.125 No Hit GTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCA 5 0.125 No Hit GATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra Read 1939862 spots for ERR5262787.sra Written 1939862 spots for ERR5262787.sra SRR ids: ['ERR5262787.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xm27wsx1 ERR5262787.sra spots: 38797240 blocks: [[1, 1939862], [1939863, 3879724], [3879725, 5819586], [5819587, 7759448], [7759449, 9699310], [9699311, 11639172], [11639173, 13579034], [13579035, 15518896], [15518897, 17458758], [17458759, 19398620], [19398621, 21338482], [21338483, 23278344], [23278345, 25218206], [25218207, 27158068], [27158069, 29097930], [29097931, 31037792], [31037793, 32977654], [32977655, 34917516], [34917517, 36857378], [36857379, 38797240]] ERR5262787 file size 12661373 ERR5262787 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262787 ERR5262787_1.fastq ERR5262787_2.fastq Input file: ERR5262787_1.fastq Paired file: ERR5262787_2.fastq trimmed: ERR5262787-trimmed-pair1.fastq, ERR5262787-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:38:02 2024 >> started Fri Dec 6 11:39:48 2024 >> done (105.417s) 38797240 read pairs processed; of these: 2 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 38797238 (100.00%) read pairs available; of these: 11477 ( 0.03%) trimmed read pairs available after processing 38785761 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 22 1 0.00% 23 1 0.00% 24 2 0.00% 25 0 0.00% 26 0 0.00% 27 1 0.00% 28 1 0.00% 29 1 0.00% 30 0 0.00% 31 3 0.00% 32 2 0.00% 33 1 0.00% 34 2 0.00% 35 3 0.00% 36 3 0.00% 37 0 0.00% 38 5 0.00% 39 4 0.00% 40 3 0.00% 41 0 0.00% 42 1 0.00% 43 4 0.00% 44 2 0.00% 45 10 0.00% 46 0 0.00% 47 2 0.00% 48 2 0.00% 49 531 0.00% 50 611 0.00% 51 651 0.00% 52 673 0.00% 53 708 0.00% 54 796 0.00% 55 897 0.00% 56 977 0.00% 57 1095 0.00% 58 1315 0.00% 59 1515 0.00% 60 1774 0.00% 61 2080 0.01% 62 2480 0.01% 63 2660 0.01% 64 2810 0.01% 65 2920 0.01% 66 3363 0.01% 67 3865 0.01% 68 4451 0.01% 69 5021 0.01% 70 5759 0.01% 71 6765 0.02% 72 7788 0.02% 73 8963 0.02% 74 9450 0.02% 75 10708 0.03% 76 11848 0.03% 77 12590 0.03% 78 13780 0.04% 79 15664 0.04% 80 17077 0.04% 81 19115 0.05% 82 21777 0.06% 83 23830 0.06% 84 26844 0.07% 85 28608 0.07% 86 30326 0.08% 87 32657 0.08% 88 34665 0.09% 89 36494 0.09% 90 39449 0.10% 91 43202 0.11% 92 46291 0.12% 93 49789 0.13% 94 53532 0.14% 95 55886 0.14% 96 58620 0.15% 97 60961 0.16% 98 63294 0.16% 99 65069 0.17% 100 67561 0.17% 101 70747 0.18% 102 74260 0.19% 103 78635 0.20% 104 81729 0.21% 105 85451 0.22% 106 88008 0.23% 107 90044 0.23% 108 90559 0.23% 109 93861 0.24% 110 96126 0.25% 111 98588 0.25% 112 101821 0.26% 113 104823 0.27% 114 109316 0.28% 115 111066 0.29% 116 112804 0.29% 117 116395 0.30% 118 116706 0.30% 119 117718 0.30% 120 120031 0.31% 121 121726 0.31% 122 123180 0.32% 123 126921 0.33% 124 131435 0.34% 125 131782 0.34% 126 133802 0.34% 127 136560 0.35% 128 136983 0.35% 129 139120 0.36% 130 139639 0.36% 131 137787 0.36% 132 142510 0.37% 133 144483 0.37% 134 146834 0.38% 135 149229 0.38% 136 151346 0.39% 137 151552 0.39% 138 152153 0.39% 139 154777 0.40% 140 156610 0.40% 141 159239 0.41% 142 161149 0.42% 143 162354 0.42% 144 166428 0.43% 145 164417 0.42% 146 169467 0.44% 147 323049 0.83% 148 157385 0.41% 149 156294 0.40% 150 31390730 80.91% 38797238 reads passed initial QC criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=46 prefix-density=0.19 prefix-fanout=2.1 sequence=GAGCGCCACCAC criterion=fanout-score sequence-density=0.03 sequence-density-rank=22 fanout-score=173.25 fanout-score-rank=1 prefix-density=0.56 prefix-fanout=9.1 sequence=CCTTCTTCTTCGTCTCCGGCGACGTCGTCTTGTCGGCGGCCTTGAGCTTGCGTGTGAGTGTGCGCCAGTAGTTCTTGATCTCGTTGTCGGTGCGGCCGGGGAGCCTCCGTGCGATGCGTGACCATCGGCTGCCCCACTGGGAGTGGAGCTGCAGGATGAGGCGCTCCTCGTCGGGCGTGATCCGGCCGCGCTTCAGCCCTGGGTGCAGGTAGTTCACCCACCGGAGACGGCAGCT criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=6.09 fanout-score-rank=28 prefix-density=0.49 prefix-fanout=3.6 sequence=TCTTCCTCCTGGCCGCCGGCGTCCTCTTCGCCGCGGCCTCCACCTCCAGCTCCAGGGAGGAGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=1178.54 fanout-score-rank=1 prefix-density=0.66 prefix-fanout=25.3 sequence=GGCGGCGGCAACGCGCAGAAGTCCAAGATGGCCAGGGAGAGGAACCTCGAGAAGCTCAAGGGCGGCAAGGGGAGCCAGCTCGAGGCCAACAAGAAGGCCATGAACATCCAGTGCAAGATATGCATGCAGACTTTCATCTGCACCACCTCTGAAGCAAAGTGCAAGGAGCACGCCGAGGCGAAGCATCCGAAGAGCGAGCTCACCCAGTGCTTCCCCCACCTCAAGCAGTGAAATCTGCCTAATCACAGTGAGAGAGAGA ERR5262787 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:40:13 Started mapping on | Dec 06 11:40:13 Finished on | Dec 06 11:43:05 Mapping speed, Million of reads per hour | 812.04 Number of input reads | 38797238 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 37510230 Uniquely mapped reads % | 96.68% Average mapped length | 288.82 Number of splices: Total | 34053720 Number of splices: Annotated (sjdb) | 31716749 Number of splices: GT/AG | 33559094 Number of splices: GC/AG | 412367 Number of splices: AT/AC | 21044 Number of splices: Non-canonical | 61215 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.24 Insertion rate per base | 0.01% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 424274 % of reads mapped to multiple loci | 1.09% Number of reads mapped to too many loci | 1080 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.21% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 862734 862734 862734 N_multimapping 424274 424274 424274 N_noFeature 1468883 36515949 1811255 N_ambiguous 768465 4820 116821 UnstrandedReadsAssigned:35272882 PositiveStrandReadsAssigned:989461 NegativeStrandReadsAssigned:35582154 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262787 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262787-trimmed-pair1.fastq ERR5262787-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 38,797,238 reads, 36,071,803 reads pseudoaligned [quant] estimated average fragment length: 260.255 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,179 rounds 52973 ERR5262787.ke.tsv 35125 ERR5262787.se.tsv 88098 total ==> ERR5262787.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 677.319 0 0 PNS24247 1044 784.745 193.5 9.80239 PNS24249 1928 1668.74 684.669 16.3106 PNS24246 1044 784.745 193.5 9.80239 PNS24248 1044 784.745 193.5 9.80239 PNS24244 1471 1211.74 174.831 5.73569 PNS24243 293 106.183 0 0 KQK14069 1603 1343.74 56630.1 1675.36 KQK14071 474 242.895 198.196 32.4381 ==> ERR5262787.se.tsv <== BRADI_1g14170v3 56844 BRADI_1g53295v3 452 BRADI_1g59795v3 728 BRADI_1g07683v3 0 BRADI_1g00485v3 34 BRADI_1g20270v3 1842 BRADI_1g74790v3 2511 BRADI_1g09890v3 0 BRADI_1g77505v3 390 BRADI_1g48960v3 0 ERR5262787 completed mapping pipeline successfully