Starting /dee2/code/volunteer_pipeline.sh ERR5262788 current disk space = 1551489134592 free memory = 1607260516 ERR5262788 SRAfilesize 31d85a008b3c00d7940989857dc19960 ERR5262788.sra ERR5262788.sra file validated ERR5262788 is paired end ERR5262788 is conventional basespace ERR5262788 read1 length is 67-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262788_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 67-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.743 37.0 37.0 37.0 37.0 37.0 2 36.6125 37.0 37.0 37.0 37.0 37.0 3 36.703 37.0 37.0 37.0 37.0 37.0 4 36.81 37.0 37.0 37.0 37.0 37.0 5 36.7235 37.0 37.0 37.0 37.0 37.0 6 36.6765 37.0 37.0 37.0 37.0 37.0 7 36.671 37.0 37.0 37.0 37.0 37.0 8 36.6565 37.0 37.0 37.0 37.0 37.0 9 36.6825 37.0 37.0 37.0 37.0 37.0 10-14 36.6918 37.0 37.0 37.0 37.0 37.0 15-19 36.6404 37.0 37.0 37.0 37.0 37.0 20-24 36.67040000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.648199999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.583 37.0 37.0 37.0 37.0 37.0 35-39 36.5635 37.0 37.0 37.0 37.0 37.0 40-44 36.5904 37.0 37.0 37.0 37.0 37.0 45-49 36.5738 37.0 37.0 37.0 37.0 37.0 50-54 36.5148 37.0 37.0 37.0 37.0 37.0 55-59 36.542100000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.5411 37.0 37.0 37.0 37.0 37.0 65-69 36.50655401350338 37.0 37.0 37.0 37.0 37.0 70-74 36.50207551887972 37.0 37.0 37.0 37.0 37.0 75-79 36.49347336834209 37.0 37.0 37.0 37.0 37.0 80-84 36.414153538384596 37.0 37.0 37.0 37.0 37.0 85-89 36.3699424856214 37.0 37.0 37.0 37.0 37.0 90-94 36.374643660915225 37.0 37.0 37.0 37.0 37.0 95-99 36.416508254127066 37.0 37.0 37.0 37.0 37.0 100-104 36.43327495621717 37.0 37.0 37.0 37.0 37.0 105-109 36.534331443268606 37.0 37.0 37.0 37.0 37.0 110-114 36.460180580264996 37.0 37.0 37.0 37.0 37.0 115-119 36.5149629875249 37.0 37.0 37.0 37.0 37.0 120-124 36.529799471188404 37.0 37.0 37.0 37.0 37.0 125-129 36.465036894147765 37.0 37.0 37.0 37.0 37.0 130-134 36.446246262595494 37.0 37.0 37.0 37.0 37.0 135-139 36.33650383329599 37.0 37.0 37.0 37.0 37.0 140-144 36.35980226893507 37.0 37.0 37.0 37.0 37.0 145-149 36.33539721663175 37.0 37.0 37.0 37.0 37.0 150 36.28048780487805 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 1.0 26 0.0 27 3.0 28 5.0 29 9.0 30 8.0 31 24.0 32 38.0 33 52.0 34 83.0 35 199.0 36 2603.0 37 975.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 7.324999999999999 67.27499999999999 1.425 23.974999999999998 2 2.775 30.95 11.15 55.125 3 16.75 19.775000000000002 19.125 44.35 4 25.2 26.700000000000003 18.8 29.299999999999997 5 18.625 38.7 16.55 26.125 6 20.549999999999997 38.75 15.575 25.124999999999996 7 11.600000000000001 33.225 35.65 19.525000000000002 8 14.975 28.325 26.525 30.175 9 18.65 28.15 27.825 25.374999999999996 10-14 20.0 34.285 21.63 24.085 15-19 20.435 31.235000000000003 22.675 25.655 20-24 19.18 28.7 22.58 29.54 25-29 20.19 29.01 22.625 28.175 30-34 22.08 29.82 22.455 25.645 35-39 20.835 30.675 22.595000000000002 25.895000000000003 40-44 17.535 31.36 26.145000000000003 24.959999999999997 45-49 18.95 30.919999999999998 23.605 26.525 50-54 18.645 29.675 25.295 26.384999999999998 55-59 18.38 27.6 26.08 27.939999999999998 60-64 19.564999999999998 29.715000000000003 27.01 23.71 65-69 22.022202220222024 29.21792179217922 25.272527252725276 23.487348734873486 70-74 21.795448862215554 28.00700175043761 24.646161540385098 25.55138784696174 75-79 21.850462615653914 27.961990497624406 21.790447611902973 28.397099274818704 80-84 21.335333833458364 32.0830207551888 19.974993748437107 26.60665166291573 85-89 25.216304076019004 27.426856714178545 25.846461615403847 21.510377594398598 90-94 23.240810202550637 26.98674668667167 25.35633908477119 24.4161040260065 95-99 24.607303651825912 26.88844422211106 22.47623811905953 26.028014007003502 100-104 20.735551663747813 29.05178884163122 25.38403802852139 24.828621466099573 105-109 23.05525121302238 18.05446861793708 27.922992643606197 30.96728752543434 110-114 16.03880559405317 27.000125992188483 30.401915081264963 26.559153332493384 115-119 21.8805817810666 27.366675172237816 27.25184996172493 23.500893084970656 120-124 21.77440123615761 24.929178470254957 23.448364666494978 29.848055627092457 125-129 18.503472677237585 21.910627702791245 34.85781679989516 24.728082820076004 130-134 12.681940700808624 24.353099730458222 28.28840970350404 34.67654986522911 135-139 21.205357142857142 19.517299107142858 30.008370535714285 29.268973214285715 140-144 26.38089758342923 20.74223245109321 32.00517836593786 20.8716915995397 145-149 22.77894107089441 20.670056835177984 30.85551899491475 25.69548309901286 150 17.759146341463413 32.31707317073171 29.039634146341463 20.884146341463413 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 2.0 29 3.0 30 4.0 31 6.0 32 7.0 33 18.0 34 23.0 35 22.5 36 32.0 37 51.0 38 69.0 39 97.0 40 126.0 41 291.5 42 382.5 43 365.0 44 354.0 45 308.0 46 307.0 47 258.0 48 175.0 49 112.5 50 89.5 51 73.5 52 73.0 53 72.0 54 66.5 55 54.0 56 40.5 57 38.5 58 43.5 59 40.0 60 33.0 61 32.5 62 39.0 63 34.0 64 30.0 65 35.0 66 28.5 67 26.0 68 24.5 69 23.5 70 19.5 71 14.5 72 17.5 73 11.5 74 6.5 75 8.5 76 4.0 77 2.0 78 2.0 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 0.0 98-99 1.0 100-101 0.0 102-103 0.0 104-105 4.0 106-107 2398.0 108-109 3.0 110-111 6.0 112-113 2.0 114-115 14.0 116-117 6.0 118-119 5.0 120-121 4.0 122-123 10.0 124-125 15.0 126-127 8.0 128-129 18.0 130-131 23.0 132-133 17.0 134-135 22.0 136-137 17.0 138-139 17.0 140-141 11.0 142-143 33.0 144-145 20.0 146-147 16.0 148-149 16.0 150-151 1312.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.975 #Duplication Level Percentage of deduplicated Percentage of total 1 64.21968461120174 29.525000000000002 2 20.663404023926045 19.0 3 7.775965198477433 10.725 4 2.718868950516585 5.0 5 0.7069059271343121 1.625 6 0.9244154431756388 2.55 7 0.5437737901033171 1.7500000000000002 8 0.3262642740619902 1.2 9 0.0543773790103317 0.22499999999999998 >10 1.7400761283306143 16.825000000000003 >50 0.27188689505165853 8.125 >100 0.0543773790103317 3.45 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACAATCAG 138 3.45 No Hit CACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCC 76 1.9 No Hit CTTTTATCAAGATACCACGCCAACAAATGGAATACGCGTCGAAAGCTGCT 69 1.725 No Hit GCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCCATT 67 1.675 No Hit CCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGA 59 1.4749999999999999 No Hit CTCCAAACTCCCAATGCAAGAGCAATTATCCAAACATGACTTAAAGACTC 54 1.35 No Hit CTCTAGTTATCCAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGC 46 1.15 No Hit ATCACAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACAATC 45 1.125 No Hit CCATCACAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACAA 44 1.0999999999999999 No Hit CCAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTATCCAA 34 0.8500000000000001 No Hit CCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGATACC 33 0.8250000000000001 No Hit CCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCCATTC 32 0.8 No Hit CCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGATAC 30 0.75 No Hit ACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCCA 30 0.75 No Hit CGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCCAT 27 0.675 No Hit GGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGATACCACG 23 0.575 No Hit CAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCCATTCA 22 0.5499999999999999 No Hit ACCACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAAC 20 0.5 No Hit CTACAGATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTT 19 0.475 No Hit ACCATCACAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACA 19 0.475 No Hit CATCACAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACAAT 18 0.44999999999999996 No Hit GCCATCACAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACA 18 0.44999999999999996 No Hit GTCCAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTATCC 17 0.42500000000000004 No Hit ACCGAACGCAAAAGTATATTGCATCCAGCGCAACTCTTGCAACTTCAAGG 17 0.42500000000000004 No Hit ATCCAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTATCC 17 0.42500000000000004 No Hit CTTACAATCAGTATATGACTTTTATCAAGATACCACGCCAACAAATGGAA 16 0.4 No Hit GTCGAAAGCTGCTGCAACTATCTAACCCATTCATTCCTCACTGGTTACGT 16 0.4 No Hit CTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGATACCA 16 0.4 No Hit GACTTTTATCAAGATACCACGCCAACAAATGGAATACGCGTCGAAAGCTG 15 0.375 No Hit CTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGAT 13 0.325 No Hit ATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAA 12 0.3 No Hit CCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAG 12 0.3 No Hit GCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGATACCACGCCA 11 0.27499999999999997 No Hit GATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCA 11 0.27499999999999997 No Hit ATCCAGCGCAACTCTTGCAACTTCAAGGAGAACTCTCCATCAGAGGTGTC 10 0.25 No Hit CAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTATCCAAA 10 0.25 No Hit CCACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACC 10 0.25 No Hit CTTAAAGACTCACCGAACGCAAAAGTATATTGCATCCAGCGCAACTCTTG 10 0.25 No Hit CCCAATGCAAGAGCAATTATCCAAACATGACTTAAAGACTCACCGAACGC 9 0.22499999999999998 No Hit CACCGAACGCAAAAGTATATTGCATCCAGCGCAACTCTTGCAACTTCAAG 8 0.2 No Hit ACTAGTTTGAGTATATGATTACGCAGGCGGGCGCCTTTCACTCCTTAGAA 8 0.2 No Hit CATGACTTAAAGACTCACCGAACGCAAAAGTATATTGCATCCAGCGCAAC 8 0.2 No Hit ATGACTTTTATCAAGATACCACGCCAACAAATGGAATACGCGTCGAAAGC 8 0.2 No Hit AACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCCATTCAT 8 0.2 No Hit CAGTATATGACTTTTATCAAGATACCACGCCAACAAATGGAATACGCGTC 8 0.2 No Hit ACAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACAATCAGT 7 0.17500000000000002 No Hit GACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTATCCAAACA 7 0.17500000000000002 No Hit CGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAGATACCACGCC 7 0.17500000000000002 No Hit ACCATTCTTACAACAGATAATCAACACTATGACAGCAAATTCACACACAG 7 0.17500000000000002 No Hit CTAGTTATCCAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAA 7 0.17500000000000002 No Hit TACAGATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTT 7 0.17500000000000002 No Hit ACGCGCCCCAGCTCCGCATCCGCACGCCCTTGTACGTCTTCATCTTGCCC 7 0.17500000000000002 No Hit CAAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTA 7 0.17500000000000002 No Hit TCCCAATGCAAGAGCAATTATCCAAACATGACTTAAAGACTCACCGAACG 7 0.17500000000000002 No Hit AAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAACCCATTCATTCC 7 0.17500000000000002 No Hit ACGGAATGATTTGTTGCGACAACCTCTCAAGAACACACGATGTTTTTTAC 6 0.15 No Hit CAAGATACCACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTA 6 0.15 No Hit AGATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATC 6 0.15 No Hit GTGGACACAGACACGGGAACAAACGCTCGAAACAATGAGGATTTACACAA 6 0.15 No Hit ACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTATCCAAACATGACTT 6 0.15 No Hit AACTGGAACTGGACTCCCAGAATATGCTATCCATCTAGAACCTAATCCAA 6 0.15 No Hit GTTATCCAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTA 6 0.15 No Hit ACTCAGACGAATGCTAGTAACACAGGCATCGTGAAGTACATAGCATGCTA 6 0.15 No Hit AATCCGTCTCCGCGATGTGCTCCATCTTCCCGTAGGGCACGATCTGGATG 6 0.15 No Hit ACAAGAACTGCCATCCGATTGAGTCCAATCGAACCTTACTTTGTGCTAGA 6 0.15 No Hit AAATAAATAACATCATTCGGTCATTACATAGTCTTAACAAATAAAGTGAG 6 0.15 No Hit CCCACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAAC 6 0.15 No Hit TCCAGACATGACTAAACTCTCCAAACTCCCAATGCAAGAGCAATTATCCA 6 0.15 No Hit ACGCGATGGAGCGAGATCTAGGATACTCGGGAGCGATAACATCACAGATA 6 0.15 No Hit GCCACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCTAAC 6 0.15 No Hit GCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTATCAAG 6 0.15 No Hit ACAGATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTATATGACTTTTA 6 0.15 No Hit AACACAACCAACAAACGGAAAGAAACTTTTTGTGCACAACCGATCAGTAA 5 0.125 No Hit ACTGGATACAACAACGCACGACCGATGCCAAGAAGTTGATCAGAGCTCTC 5 0.125 No Hit CCTCTCCAAACTCCCAATGCAAGAGCAATTATCCAAACATGACTTAAAGA 5 0.125 No Hit GCTTACAATCAGTATATGACTTTTATCAAGATACCACGCCAACAAATGGA 5 0.125 No Hit ACCCACTTGTGTGCATCTTCTTCGCTGTCACTATACTGATGAGCAGTGTC 5 0.125 No Hit ACGCCGGCATCCTGCTTCCGCTGCCGCGGCAGATAGAGCTTAGCCTAGCC 5 0.125 No Hit ACGTCTTCGAACTCCCCGTTGAGCGTCGAGCCGAGGATGGCCGCGACGCA 5 0.125 No Hit ACTGGAACGATCAATTGCGCGACACATATGTACCGATTTTCCTGATCGTG 5 0.125 No Hit GATACCACGCCAACAAATGGAATACGCGTCGAAAGCTGCTGCAACTATCT 5 0.125 No Hit ACTATGCATGGATGGAACGGGAAGAACCACTACACAAGATAAACGTCGTA 5 0.125 No Hit CTCCCAATGCAAGAGCAATTATCCAAACATGACTTAAAGACTCACCGAAC 5 0.125 No Hit AAATTTAACTACAGATCCTCCCTGGCGCACAGAAAGCTTACAATCAGTAT 5 0.125 No Hit CCAAACATGACTTAAAGACTCACCGAACGCAAAAGTATATTGCATCCAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTCTC 30 1.4988229E-5 52.016666 140-144 CTCTTCC 40 0.003981394 31.21 130-134 CCTCAGC 40 0.003981394 31.21 135-139 GCGGATC 50 3.8504176E-4 30.009615 120-124 GACGAGC 50 4.4767477E-4 29.259375 115-119 GCTTCGA 50 4.4767477E-4 29.259375 110-114 GTTAAGC 55 7.8200444E-4 26.59943 105-109 AACTACA 30 0.005008465 19.50625 9 ACGCTGT 60 0.005916 18.846619 100-104 >>END_MODULE ERR5262788 read2 length is 67-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262788_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 67-150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4505 37.0 37.0 37.0 37.0 37.0 2 36.1175 37.0 37.0 37.0 37.0 37.0 3 36.312 37.0 37.0 37.0 37.0 37.0 4 36.2935 37.0 37.0 37.0 37.0 37.0 5 36.346 37.0 37.0 37.0 37.0 37.0 6 36.451 37.0 37.0 37.0 37.0 37.0 7 36.449 37.0 37.0 37.0 37.0 37.0 8 36.407 37.0 37.0 37.0 37.0 37.0 9 36.493 37.0 37.0 37.0 37.0 37.0 10-14 36.4494 37.0 37.0 37.0 37.0 37.0 15-19 36.4973 37.0 37.0 37.0 37.0 37.0 20-24 36.42 37.0 37.0 37.0 37.0 37.0 25-29 36.4017 37.0 37.0 37.0 37.0 37.0 30-34 36.3859 37.0 37.0 37.0 37.0 37.0 35-39 36.3731 37.0 37.0 37.0 37.0 37.0 40-44 36.3968 37.0 37.0 37.0 37.0 37.0 45-49 36.411500000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.3503 37.0 37.0 37.0 37.0 37.0 55-59 36.2856 37.0 37.0 37.0 37.0 37.0 60-64 36.2853 37.0 37.0 37.0 37.0 37.0 65-69 36.26112688172043 37.0 37.0 37.0 37.0 37.0 70-74 36.185496374093525 37.0 37.0 37.0 37.0 37.0 75-79 36.157289322330584 37.0 37.0 37.0 37.0 37.0 80-84 36.0946736684171 37.0 37.0 37.0 37.0 37.0 85-89 36.141785446361595 37.0 37.0 37.0 37.0 37.0 90-94 36.08786302468004 37.0 37.0 37.0 37.0 37.0 95-99 36.11619524405506 37.0 37.0 37.0 37.0 37.0 100-104 36.012118177265904 37.0 37.0 37.0 37.0 37.0 105-109 35.964018724285154 37.0 37.0 37.0 37.0 37.0 110-114 35.79671424360929 37.0 37.0 37.0 37.0 37.0 115-119 35.78195457243511 37.0 37.0 37.0 37.0 37.0 120-124 35.775867414701324 37.0 37.0 37.0 37.0 37.0 125-129 35.71871479728041 37.0 37.0 37.0 37.0 37.0 130-134 35.73516439694607 37.0 37.0 37.0 37.0 37.0 135-139 35.75138450547396 37.0 37.0 37.0 37.0 37.0 140-144 35.694692822749175 37.0 37.0 37.0 37.0 37.0 145-149 35.51838361542309 37.0 37.0 37.0 37.0 37.0 150 35.63262195121951 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 0.0 13 0.0 14 0.0 15 1.0 16 0.0 17 0.0 18 0.0 19 2.0 20 1.0 21 4.0 22 3.0 23 4.0 24 2.0 25 4.0 26 2.0 27 6.0 28 6.0 29 11.0 30 16.0 31 23.0 32 35.0 33 76.0 34 108.0 35 395.0 36 2643.0 37 656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.275 22.825 7.1 25.8 2 28.775000000000002 24.9 26.85 19.475 3 21.6 24.825 31.1 22.475 4 25.650000000000002 29.15 22.650000000000002 22.55 5 29.849999999999998 33.5 16.525000000000002 20.125 6 20.825 35.449999999999996 19.650000000000002 24.075 7 24.125 20.3 32.574999999999996 23.0 8 22.95 23.674999999999997 25.124999999999996 28.249999999999996 9 25.3 23.5 25.174999999999997 26.025 10-14 25.490000000000002 25.46 24.035 25.014999999999997 15-19 25.724999999999998 24.775 25.195 24.305 20-24 25.295 24.51 24.834999999999997 25.36 25-29 26.96 25.22 23.75 24.07 30-34 25.4 25.365 26.05 23.185 35-39 25.25 24.3 25.885 24.565 40-44 25.069999999999997 25.525 26.040000000000003 23.365 45-49 24.89 25.0 25.919999999999998 24.19 50-54 25.715 25.240000000000002 24.85 24.195 55-59 25.1 24.385 26.11 24.404999999999998 60-64 24.965 24.97 26.965 23.1 65-69 25.95259525952595 24.202420242024203 26.672667266726673 23.17231723172317 70-74 26.386596649162293 25.726431607901972 25.936484121030258 21.950487621905477 75-79 25.26631657914479 25.2863215803951 26.926731682920728 22.520630157539383 80-84 26.31157789447362 25.516379094773693 26.426606651662915 21.745436359089773 85-89 27.291822955738937 25.221305326331585 26.531632908227053 20.955238809702426 90-94 26.055633380028016 25.660396237742646 26.691014608765258 21.59295577346408 95-99 26.89862327909887 24.29536921151439 27.108886107634543 21.697121401752188 100-104 25.332999499248878 24.381572358537806 27.43615423134702 22.849273910866298 105-109 28.217976824303165 14.688380833072346 42.62449107422487 14.469151268399624 110-114 26.622149426735543 23.056570492629458 27.541892402671035 22.779387677963967 115-119 24.94258739474356 22.862975248787958 30.99004848175555 21.204388874712937 120-124 26.02369302086016 24.633015709502963 26.770538243626063 22.572753026010815 125-129 26.431660332852836 23.18175861617088 29.747084261564673 20.63949678941161 130-134 25.822102425876007 23.463611859838274 28.80053908355795 21.91374663072776 135-139 26.827566964285715 23.186383928571427 29.966517857142854 20.01953125 140-144 28.754315304948214 23.50402761795167 27.876869965477564 19.864787111622555 145-149 24.63356266826204 27.25097218067604 27.81932396051451 20.296141190547413 150 28.20121951219512 23.09451219512195 27.820121951219512 20.884146341463413 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.5 21 2.0 22 1.0 23 0.0 24 0.5 25 1.0 26 0.5 27 0.0 28 0.5 29 1.5 30 5.0 31 7.0 32 9.5 33 19.0 34 20.0 35 21.5 36 36.5 37 43.5 38 57.5 39 82.5 40 116.0 41 150.0 42 209.5 43 240.0 44 230.5 45 189.5 46 176.0 47 196.5 48 239.0 49 222.5 50 157.0 51 141.0 52 124.5 53 125.0 54 106.5 55 126.0 56 105.5 57 84.0 58 104.5 59 104.5 60 108.5 61 81.0 62 45.5 63 33.0 64 34.5 65 42.0 66 33.0 67 17.0 68 26.0 69 25.5 70 15.5 71 15.0 72 17.0 73 15.0 74 8.0 75 7.5 76 7.0 77 4.5 78 2.5 79 1.0 80 0.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.0 92-93 2.0 94-95 1.0 96-97 0.0 98-99 1.0 100-101 0.0 102-103 0.0 104-105 4.0 106-107 2395.0 108-109 3.0 110-111 6.0 112-113 2.0 114-115 14.0 116-117 6.0 118-119 5.0 120-121 4.0 122-123 10.0 124-125 15.0 126-127 8.0 128-129 18.0 130-131 23.0 132-133 17.0 134-135 22.0 136-137 17.0 138-139 17.0 140-141 11.0 142-143 33.0 144-145 20.0 146-147 16.0 148-149 16.0 150-151 1312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.75 #Duplication Level Percentage of deduplicated Percentage of total 1 62.83720930232558 33.775 2 20.46511627906977 22.0 3 8.093023255813954 13.05 4 3.488372093023256 7.5 5 1.5348837209302326 4.125 6 0.9767441860465116 3.15 7 0.5581395348837209 2.1 8 0.6046511627906976 2.6 9 0.27906976744186046 1.35 >10 1.1627906976744187 10.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCTAT 47 1.175 No Hit GGAAGAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCA 30 0.75 No Hit GCTGAGGAAGAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCAT 23 0.575 No Hit GTTGCCGTTCCCTTGGTCCAAAGGAAACAACTGAACTTAGTTGTCAGGAC 21 0.525 No Hit GCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTTCTGA 21 0.525 No Hit ATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTT 18 0.44999999999999996 No Hit GTGACAGAGTGCATTGGTGGATCCCGAACCGTGACCTTCGATGACCTGGG 17 0.42500000000000004 No Hit GCTACCACACCCACTGTGACCCGAGGCTGAATGCATCCCAGTCTCTGGAG 17 0.42500000000000004 No Hit CAGCGTCTTGCCATTGCCAACCTATGGTTTCTGAAACACGTAACCAGTGA 16 0.4 No Hit GAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCT 16 0.4 No Hit CGCTACCACACCCACTGTGACCCGAGGCTGAATGCATCCCAGTCTCTGGA 16 0.4 No Hit CTTCATCATTGCTGAGAAGCTGAGGAAGAGGAGGATCCGCTCGTCGAAGC 15 0.375 No Hit CCCACTGTGACCCGAGGCTGAATGCATCCCAGTCTCTGGAGCTCTCCTTC 15 0.375 No Hit CATTGCTGAGAAGCTGAGGAAGAGGAGGATCCGCTCGTCGAAGCTTAACA 15 0.375 No Hit GAATGCATCCCAGTCTCTGGAGCTCTCCTTCATCATTGCTGAGAAGCTGA 14 0.35000000000000003 No Hit GGTTTCTGAAACACGTAACCAGTGAGGAATGAATGGGTTAGATAGTTGCA 13 0.325 No Hit GGAGCTCTCCTTCATCATTGCTGAGAAGCTGAGGAAGAGGAGGATCCGCT 13 0.325 No Hit GGTCAGGGCGTTCTTCGATGTTCATGAGCAAGAAGGTAGCCACGCAGGAG 13 0.325 No Hit GGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCTATGGT 12 0.3 No Hit GGGCGTTCTTCGATGTTCATGAGCAAGAAGGTAGCCACGCAGGAGGCGTC 12 0.3 No Hit ATTGAACCCAACAAACAAGCCTGGGAGAATTACCATCATTACAAGAATGG 10 0.25 No Hit CTGGAGCTCTCCTTCATCATTGCTGAGAAGCTGAGGAAGAGGAGGATCCG 10 0.25 No Hit GGAAACAACTGAACTTAGTTGTCAGGACCAAAACCGTATTTTTGAACATA 10 0.25 No Hit CCCGAACCGTGACCTTCGATGACCTGGGCGACCGCTACCACACCCACTGT 10 0.25 No Hit GAGGAATGAATGGGTTAGATAGTTGCAGCAGCTTTCGACGCGTATTCCAT 10 0.25 No Hit GAATGAATGGGTTAGATAGTTGCAGCAGCTTTCGACGCGTATTCCATTTG 9 0.22499999999999998 No Hit GTGCATTGGTGGATCCCGAACCGTGACCTTCGATGACCTGGGCGACCGCT 9 0.22499999999999998 No Hit ACTGAACTTAGTTGTCAGGACCAAAACCGTATTTTTGAACATAGACACCT 9 0.22499999999999998 No Hit AGCTGAGGAAGAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCA 9 0.22499999999999998 No Hit GAAACACGTAACCAGTGAGGAATGAATGGGTTAGATAGTTGCAGCAGCTT 9 0.22499999999999998 No Hit GAGGAAGAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGC 9 0.22499999999999998 No Hit CGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTTCTG 8 0.2 No Hit CTGAGGAAGAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATT 8 0.2 No Hit CACGTAACCAGTGAGGAATGAATGGGTTAGATAGTTGCAGCAGCTTTCGA 8 0.2 No Hit AACAGCGTCTTGCCATTGCCAACCTATGGTTTCTGAAACACGTAACCAGT 8 0.2 No Hit GGTAGCCACGCAGGAGGCGTCCACCTTGAGATGACTGGGCAGAATGTGAC 8 0.2 No Hit CAGTCTCTGGAGCTCTCCTTCATCATTGCTGAGAAGCTGAGGAAGAGGAG 8 0.2 No Hit GTAGAGAAGCTTAGGGTCACCGAGTCCAAAAAGGATGAAAACGATGATAA 8 0.2 No Hit CGAACCGTGACCTTCGATGACCTGGGCGACCGCTACCACACCCACTGTGA 8 0.2 No Hit GGTGGATCCCGAACCGTGACCTTCGATGACCTGGGCGACCGCTACCACAC 8 0.2 No Hit GCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTTCTGAAACACGTAAC 8 0.2 No Hit AATGCATCCCAGTCTCTGGAGCTCTCCTTCATCATTGCTGAGAAGCTGAG 8 0.2 No Hit GAATTGTTGCCGTTCCCTTGGTCCAAAGGAAACAACTGAACTTAGTTGTC 8 0.2 No Hit GTTTCTGAAACACGTAACCAGTGAGGAATGAATGGGTTAGATAGTTGCAG 8 0.2 No Hit AATGAATGGGTTAGATAGTTGCAGCAGCTTTCGACGCGTATTCCATTTGT 7 0.17500000000000002 No Hit TATGGTTTCTGAAACACGTAACCAGTGAGGAATGAATGGGTTAGATAGTT 7 0.17500000000000002 No Hit CTCCATTCTGGCTGAGGTCAGGGCGTTCTTCGATGTTCATGAGCAAGAAG 7 0.17500000000000002 No Hit ATTAGAAGTCGGGCAAATGGTCGGCGCTAATTCTTCTGTGACTTGTTTTT 7 0.17500000000000002 No Hit GGGCAGAATGTGACAGAGTGCATTGGTGGATCCCGAACCGTGACCTTCGA 7 0.17500000000000002 No Hit GCGCACGATACAAGTAATGGAGCTGGGTGACATACCAAAGCCTAAGAATC 7 0.17500000000000002 No Hit AGCTCTCCTTCATCATTGCTGAGAAGCTGAGGAAGAGGAGGATCCGCTCG 7 0.17500000000000002 No Hit GACCGCTACCACACCCACTGTGACCCGAGGCTGAATGCATCCCAGTCTCT 7 0.17500000000000002 No Hit GGCAGAATGTGACAGAGTGCATTGGTGGATCCCGAACCGTGACCTTCGAT 7 0.17500000000000002 No Hit GCGCGGAGCAATGGCGCCTCCTCGGCGGCGAAGCCGCAGCAGCAGCAGCA 7 0.17500000000000002 No Hit TGCTGAGAAGCTGAGGAAGAGGAGGATCCGCTCGTCGAAGCTTAACAGCG 7 0.17500000000000002 No Hit GGTAGATCATCGATCAGACATTTCTTGAGCTTGTGTGGTTCCTTTAATCA 7 0.17500000000000002 No Hit AAGCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTTCTGAAACACGTA 6 0.15 No Hit CTTCGATGACCTGGGCGACCGCTACCACACCCACTGTGACCCGAGGCTGA 6 0.15 No Hit GTATTTTTGAACATAGACACCTCTGATGGAGAGTTCTCCTTGAAGTTGCA 6 0.15 No Hit ATCCACCATCCCTCCTTCTCTTTCTCTTCTCCATCATCTCTTTAAACAAA 6 0.15 No Hit GCAGAATGTGACAGAGTGCATTGGTGGATCCCGAACCGTGACCTTCGATG 6 0.15 No Hit AGCGTCTTGCCATTGCCAACCTATGGTTTCTGAAACACGTAACCAGTGAG 6 0.15 No Hit GTTTGCTCTGTTAGAGAGGAAGAATAATATGCATGCCTACATCTTTCATA 6 0.15 No Hit CCTATGGTTTCTGAAACACGTAACCAGTGAGGAATGAATGGGTTAGATAG 6 0.15 No Hit CCTGGGCGACCGCTACCACACCCACTGTGACCCGAGGCTGAATGCATCCC 6 0.15 No Hit GTTTTTCTTCACCTGATGAATTGTTGCCGTTCCCTTGGTCCAAAGGAAAC 6 0.15 No Hit GACGTCCACGGCGCCGGGCTGACAAACTCCCTGTTCCTCCCCACGGGGGC 6 0.15 No Hit GTTAATGTGTATATTTGCATTGTTGGTAATTAATTACTAGAGGTAGATGG 6 0.15 No Hit CCGAGGCTGAATGCATCCCAGTCTCTGGAGCTCTCCTTCATCATTGCTGA 6 0.15 No Hit AGCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTTCTGAAACACGTAA 6 0.15 No Hit GGACTATACTCTTCTTACCAGCCCTATAGTTTCACCAATCTCAGGTTATG 6 0.15 No Hit GTCGGCGCTAATTCTTCTGTGACTTGTTTTTCTTCACCTGATGAATTGTT 6 0.15 No Hit CAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTT 6 0.15 No Hit AACTGAACTTAGTTGTCAGGACCAAAACCGTATTTTTGAACATAGACACC 6 0.15 No Hit CTGTGCTATGTGCTGGGTTTAAGCTTGAGGATGGAGGCGCTTCTGCTGCT 6 0.15 No Hit TGTTGCCGTTCCCTTGGTCCAAAGGAAACAACTGAACTTAGTTGTCAGGA 6 0.15 No Hit GGCAAATGGTCGGCGCTAATTCTTCTGTGACTTGTTTTTCTTCACCTGAT 6 0.15 No Hit GGTCGTGCCGTTGGAGGCCGTGTGCACGCCGCGTGGAACCATGCTTCGTG 5 0.125 No Hit ACGATACAAGTAATGGAGCTGGGTGACATACCAAAGCCTAAGAATCATAA 5 0.125 No Hit CTGAACTTAGTTGTCAGGACCAAAACCGTATTTTTGAACATAGACACCTC 5 0.125 No Hit GAATGTGACAGAGTGCATTGGTGGATCCCGAACCGTGACCTTCGATGACC 5 0.125 No Hit GTGACCCGAGGCTGAATGCATCCCAGTCTCTGGAGCTCTCCTTCATCATT 5 0.125 No Hit CTTGAGATGACTGGGCAGAATGTGACAGAGTGCATTGGTGGATCCCGAAC 5 0.125 No Hit AGGAAGAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCC 5 0.125 No Hit GCCATTGCCAACCTATGGTTTCTGAAACACGTAACCAGTGAGGAATGAAT 5 0.125 No Hit CGTAACCAGTGAGGAATGAATGGGTTAGATAGTTGCAGCAGCTTTCGACG 5 0.125 No Hit GGAAAATCCGGTGTGATGGTCGTACGGAGGAGGCTCAGGCGAATCAGACG 5 0.125 No Hit CAACAGCAAGCACAATAAACTTTATTGACAGTGCAACTGATAACCTCGTA 5 0.125 No Hit CCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTTCT 5 0.125 No Hit GTAAACGTGGCCAAGTTTACAAATTCCTTCGTTCGCAAGGTTTTGTTTCC 5 0.125 No Hit GGTTTGGAAAGCAACGGAAGGCTAGAGGTAGCGAAACGTAAACTCCAGGA 5 0.125 No Hit GTCTCTGGAGCTCTCCTTCATCATTGCTGAGAAGCTGAGGAAGAGGAGGA 5 0.125 No Hit CGATGACCTGGGCGACCGCTACCACACCCACTGTGACCCGAGGCTGAATG 5 0.125 No Hit AGAGGAGGATCCGCTCGTCGAAGCTTAACAGCGTCTTGCCATTGCCAACC 5 0.125 No Hit TGGTGGATCCCGAACCGTGACCTTCGATGACCTGGGCGACCGCTACCACA 5 0.125 No Hit AAATGGTCTCGCACCGCTAGCATCGGAGCGGCCCGTACTTGATAATACAT 5 0.125 No Hit CCTTGAGATGACTGGGCAGAATGTGACAGAGTGCATTGGTGGATCCCGAA 5 0.125 No Hit CACACCCACTGTGACCCGAGGCTGAATGCATCCCAGTCTCTGGAGCTCTC 5 0.125 No Hit CCGGCAACCTATTATGAAGTCAAGGAATAACATTAGAAGTCGGGCAAATG 5 0.125 No Hit ACAAGAAGTTACTGATCGGTTGTGGTGGATATTTCAGTTACCAGAAGCAC 5 0.125 No Hit GTTACCTCATCTTATCCGTGCTGTCCGCCATGCTGGTCAAATTGTCACCT 5 0.125 No Hit AACCGGCAACCTATTATGAAGTCAAGGAATAACATTAGAAGTCGGGCAAA 5 0.125 No Hit GGGCAAATGGTCGGCGCTAATTCTTCTGTGACTTGTTTTTCTTCACCTGA 5 0.125 No Hit AATAAGGGCAGCTCCTCAGCAGAAGCCTGTCGGTGAGATGAAATACAAAC 5 0.125 No Hit AGTGCATTGGTGGATCCCGAACCGTGACCTTCGATGACCTGGGCGACCGC 5 0.125 No Hit GGATCCCGAACCGTGACCTTCGATGACCTGGGCGACCGCTACCACACCCA 5 0.125 No Hit GTCGAAGCTTAACAGCGTCTTGCCATTGCCAACCTATGGTTTCTGAAACA 5 0.125 No Hit GGGCGACCGCTACCACACCCACTGTGACCCGAGGCTGAATGCATCCCAGT 5 0.125 No Hit GTGACCTTCGATGACCTGGGCGACCGCTACCACACCCACTGTGACCCGAG 5 0.125 No Hit GGTACTACGTGATGGACCCCGAGAAGGCCGTGGAGCTGGTCGACGAGAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTTTC 60 0.0012996678 24.382812 105-109 >>END_MODULE Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474939 spots for ERR5262788.sra Written 1474939 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra Read 1474934 spots for ERR5262788.sra Written 1474934 spots for ERR5262788.sra SRR ids: ['ERR5262788.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uv1j0ia1 ERR5262788.sra spots: 29498685 blocks: [[1, 1474934], [1474935, 2949868], [2949869, 4424802], [4424803, 5899736], [5899737, 7374670], [7374671, 8849604], [8849605, 10324538], [10324539, 11799472], [11799473, 13274406], [13274407, 14749340], [14749341, 16224274], [16224275, 17699208], [17699209, 19174142], [19174143, 20649076], [20649077, 22124010], [22124011, 23598944], [23598945, 25073878], [25073879, 26548812], [26548813, 28023746], [28023747, 29498685]] ERR5262788 file size 9512467 ERR5262788 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262788 ERR5262788_1.fastq ERR5262788_2.fastq Input file: ERR5262788_1.fastq Paired file: ERR5262788_2.fastq trimmed: ERR5262788-trimmed-pair1.fastq, ERR5262788-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:38:31 2024 >> started Fri Dec 6 11:39:02 2024 >> done (31.190s) 29498685 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 29498684 (100.00%) read pairs available; of these: 10494 ( 0.04%) trimmed read pairs available after processing 29488190 (99.96%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 1 0.00% 29 1 0.00% 30 2 0.00% 31 1 0.00% 32 1 0.00% 33 1 0.00% 34 1 0.00% 35 1 0.00% 36 2 0.00% 37 4 0.00% 38 2 0.00% 39 2 0.00% 40 5 0.00% 41 2 0.00% 42 2 0.00% 43 1 0.00% 44 0 0.00% 45 3 0.00% 46 3 0.00% 47 4 0.00% 48 3 0.00% 49 684 0.00% 50 839 0.00% 51 839 0.00% 52 977 0.00% 53 1069 0.00% 54 1133 0.00% 55 1257 0.00% 56 1439 0.00% 57 1671 0.01% 58 1855 0.01% 59 2288 0.01% 60 2600 0.01% 61 2982 0.01% 62 3302 0.01% 63 3715 0.01% 64 4195 0.01% 65 4267 0.01% 66 4860 0.02% 67 5508 0.02% 68 6296 0.02% 69 7149 0.02% 70 8376 0.03% 71 9269 0.03% 72 10656 0.04% 73 11886 0.04% 74 13181 0.04% 75 14619 0.05% 76 15626 0.05% 77 16673 0.06% 78 18430 0.06% 79 20231 0.07% 80 22762 0.08% 81 25411 0.09% 82 27967 0.09% 83 30710 0.10% 84 33427 0.11% 85 35595 0.12% 86 37684 0.13% 87 40210 0.14% 88 42075 0.14% 89 44759 0.15% 90 46874 0.16% 91 50175 0.17% 92 53685 0.18% 93 57981 0.20% 94 61524 0.21% 95 63204 0.21% 96 66242 0.22% 97 67858 0.23% 98 70084 0.24% 99 72143 0.24% 100 73992 0.25% 101 75989 0.26% 102 79649 0.27% 103 83465 0.28% 104 85288 0.29% 105 88872 0.30% 106 91054 0.31% 107 92945 0.32% 108 93698 0.32% 109 95605 0.32% 110 96676 0.33% 111 99014 0.34% 112 102184 0.35% 113 104230 0.35% 114 107157 0.36% 115 108252 0.37% 116 109796 0.37% 117 111793 0.38% 118 112657 0.38% 119 112827 0.38% 120 113982 0.39% 121 115695 0.39% 122 116404 0.39% 123 119178 0.40% 124 121920 0.41% 125 123026 0.42% 126 124356 0.42% 127 124761 0.42% 128 125127 0.42% 129 126707 0.43% 130 126163 0.43% 131 125876 0.43% 132 127930 0.43% 133 130336 0.44% 134 131591 0.45% 135 133429 0.45% 136 134457 0.46% 137 134006 0.45% 138 133951 0.45% 139 135427 0.46% 140 133609 0.45% 141 135843 0.46% 142 139387 0.47% 143 137867 0.47% 144 140062 0.47% 145 140586 0.48% 146 142394 0.48% 147 248710 0.84% 148 135554 0.46% 149 132493 0.45% 150 22414427 75.98% 29498684 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=4.50 fanout-score-rank=28 prefix-density=0.43 prefix-fanout=3.4 sequence=GATCGATCGATC criterion=fanout-score sequence-density=0.08 sequence-density-rank=17 fanout-score=241.16 fanout-score-rank=1 prefix-density=0.98 prefix-fanout=18.7 sequence=GCGGCGGCGGCCTTCTTGCCATCCTTGTCGAAGATCTTGTTGAGCCCGGGGAGAACCTTGGTCTTCCAAACGCTTGTCATCTTTCTGTCTTTCAAATATCTATGGAGCAGCCGAAGCCTGAAGAGGTAGAGATGTGTAGCTTAGCTAAGCTCCTCCTCCAAATGATTTTCGCGATGTGTAGAAGTGATCACCAACAACCAGCCTACCAATCAATGGG criterion=sequence-density sequence-density=0.25 sequence-density-rank=1 fanout-score=2.26 fanout-score-rank=29 prefix-density=0.26 prefix-fanout=2.2 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.12 sequence-density-rank=12 fanout-score=193.68 fanout-score-rank=1 prefix-density=1.07 prefix-fanout=22.6 sequence=CGCCGCCGCCGC ERR5262788 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:39:31 Started mapping on | Dec 06 11:39:31 Finished on | Dec 06 11:41:35 Mapping speed, Million of reads per hour | 856.41 Number of input reads | 29498684 Average input read length | 285 UNIQUE READS: Uniquely mapped reads number | 28648102 Uniquely mapped reads % | 97.12% Average mapped length | 285.10 Number of splices: Total | 25719649 Number of splices: Annotated (sjdb) | 24018174 Number of splices: GT/AG | 25368435 Number of splices: GC/AG | 288774 Number of splices: AT/AC | 16348 Number of splices: Non-canonical | 46092 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.50 Insertion rate per base | 0.01% Insertion average length | 2.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 308868 % of reads mapped to multiple loci | 1.05% Number of reads mapped to too many loci | 784 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.83% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 541714 541714 541714 N_multimapping 308868 308868 308868 N_noFeature 1121894 27906398 1371956 N_ambiguous 574565 3358 83074 UnstrandedReadsAssigned:26951643 PositiveStrandReadsAssigned:738346 NegativeStrandReadsAssigned:27193072 Dataset is classified negative stranded MeadianReadLen=100 20thPercentileLength=96 echo kmer=91 ERR5262788 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262788-trimmed-pair1.fastq ERR5262788-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,498,684 reads, 27,490,410 reads pseudoaligned [quant] estimated average fragment length: 123.145 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,217 rounds 52973 ERR5262788.ke.tsv 35125 ERR5262788.se.tsv 88098 total ==> ERR5262788.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 814.004 0 0 PNS24247 1044 921.855 166.957 10.9411 PNS24249 1928 1805.85 479.56 16.0428 PNS24246 1044 921.855 166.957 10.9411 PNS24248 1044 921.855 166.957 10.9411 PNS24244 1471 1348.85 245.568 10.9983 PNS24243 293 182.239 0 0 KQK14069 1603 1480.85 18582.3 758.063 KQK14071 474 357.448 558.443 94.3812 ==> ERR5262788.se.tsv <== BRADI_1g14170v3 21279 BRADI_1g53295v3 164 BRADI_1g59795v3 518 BRADI_1g07683v3 0 BRADI_1g00485v3 40 BRADI_1g20270v3 1351 BRADI_1g74790v3 2265 BRADI_1g09890v3 0 BRADI_1g77505v3 264 BRADI_1g48960v3 0 ERR5262788 completed mapping pipeline successfully