Starting /dee2/code/volunteer_pipeline.sh ERR5262789 current disk space = 1551514374144 free memory = 1602826472 ERR5262789 SRAfilesize 2275e076f4fea878f26e1181be958dc9 ERR5262789.sra ERR5262789.sra file validated ERR5262789 is paired end ERR5262789 is conventional basespace ERR5262789 read1 length is 84-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262789_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 84-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.755 37.0 37.0 37.0 37.0 37.0 2 36.324 37.0 37.0 37.0 37.0 37.0 3 36.555 37.0 37.0 37.0 37.0 37.0 4 36.7305 37.0 37.0 37.0 37.0 37.0 5 36.8025 37.0 37.0 37.0 37.0 37.0 6 36.731 37.0 37.0 37.0 37.0 37.0 7 36.741 37.0 37.0 37.0 37.0 37.0 8 36.7655 37.0 37.0 37.0 37.0 37.0 9 36.812 37.0 37.0 37.0 37.0 37.0 10-14 36.7668 37.0 37.0 37.0 37.0 37.0 15-19 36.5525 37.0 37.0 37.0 37.0 37.0 20-24 36.5505 37.0 37.0 37.0 37.0 37.0 25-29 36.571400000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.1745 37.0 37.0 37.0 37.0 37.0 35-39 36.35809999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.7045 37.0 37.0 37.0 37.0 37.0 45-49 36.6934 37.0 37.0 37.0 37.0 37.0 50-54 36.7428 37.0 37.0 37.0 37.0 37.0 55-59 36.599199999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.1916 37.0 37.0 37.0 37.0 37.0 65-69 36.497600000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.6785 37.0 37.0 37.0 37.0 37.0 75-79 36.705799999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.6464 37.0 37.0 37.0 37.0 37.0 85-89 36.51097908698803 37.0 37.0 37.0 37.0 37.0 90-94 36.515080159249045 37.0 37.0 37.0 37.0 37.0 95-99 36.455369870145475 37.0 37.0 37.0 37.0 37.0 100-104 36.592410132710484 37.0 37.0 37.0 37.0 37.0 105-109 36.635663770095576 37.0 37.0 37.0 37.0 37.0 110-114 36.61126554216064 37.0 37.0 37.0 37.0 37.0 115-119 36.08411622742048 37.0 37.0 37.0 37.0 37.0 120-124 35.988760625038495 37.0 37.0 37.0 37.0 37.0 125-129 36.24698188173993 37.0 37.0 37.0 37.0 37.0 130-134 36.55031852669855 37.0 37.0 37.0 37.0 37.0 135-139 36.39405908058515 37.0 37.0 37.0 37.0 37.0 140-144 36.075675849210974 37.0 37.0 37.0 34.6 37.0 145-149 36.29516786963103 37.0 37.0 37.0 37.0 37.0 150 36.42612064194798 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 0.0 25 1.0 26 0.0 27 0.0 28 0.0 29 6.0 30 6.0 31 14.0 32 24.0 33 43.0 34 81.0 35 209.0 36 3276.0 37 338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 64.125 12.325 18.224999999999998 5.325 2 25.18759379689845 7.728864432216108 45.397698849424714 21.68584292146073 3 8.225 6.4750000000000005 52.25 33.050000000000004 4 6.425 26.625 46.975 19.975 5 21.95 42.3 25.674999999999997 10.075000000000001 6 10.174999999999999 27.875 54.35 7.6 7 2.375 11.25 82.35 4.025 8 10.0 11.924999999999999 30.875000000000004 47.199999999999996 9 5.25 33.4 13.200000000000001 48.15 10-14 24.755 36.41 17.8 21.035 15-19 23.48 31.155 23.87 21.495 20-24 14.7 33.055 33.955 18.29 25-29 13.985 41.260000000000005 16.655 28.1 30-34 13.100000000000001 37.525 15.909999999999998 33.465 35-39 18.96 31.535000000000004 19.275000000000002 30.23 40-44 23.515 23.89 29.17 23.425 45-49 35.725 21.68 10.489999999999998 32.105 50-54 37.035000000000004 11.97 19.935 31.06 55-59 39.324999999999996 25.919999999999998 9.805 24.95 60-64 14.979999999999999 50.525 12.31 22.185 65-69 30.135 20.16 34.510000000000005 15.195 70-74 6.84 18.065 55.85 19.245 75-79 28.315 13.995 39.065 18.625 80-84 23.150000000000002 49.004999999999995 8.774999999999999 19.07 85-89 31.163372529397048 45.95446584938704 10.162621966474857 12.719539654741055 90-94 27.03894932076796 37.93673868364329 19.539826557722193 15.48448543786656 95-99 16.42225793491362 54.550020088388905 15.75431900361591 13.273402973081557 100-104 29.315386166565837 32.173825368017745 10.92458156886469 27.586206896551722 105-109 28.967993123325076 40.27405572129241 6.082823481822318 24.675127673560198 110-114 29.07585225829396 21.99867906315094 32.14449016918153 16.780978509373572 115-119 22.06752345981232 29.32986536107711 28.314973480212156 20.287637698898408 120-124 12.698169172547816 22.9057993249463 49.31471821622174 15.081313286284137 125-129 9.084822578153165 19.00396559715713 55.672863985167645 16.238347839522067 130-134 35.22768670309654 12.896174863387976 41.96200884725475 9.914129586260733 135-139 29.041884816753928 16.602094240837697 35.26701570680628 19.089005235602095 140-144 29.38556963367439 21.062397285691564 27.04765943911361 22.504373641520438 145-149 47.871303217419566 5.681941284801213 34.97995883436247 11.466796663416748 150 48.561151079136685 3.624792473713337 40.95185390149419 6.862202545655784 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 0.0 28 0.0 29 0.5 30 1.0 31 1.0 32 1.0 33 1.5 34 1.5 35 0.5 36 3.5 37 7.5 38 9.0 39 8.0 40 81.5 41 711.0 42 722.0 43 114.0 44 28.0 45 8.0 46 29.5 47 143.0 48 974.5 49 922.0 50 144.0 51 80.0 52 3.0 53 1.5 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 84-85 1.0 86-87 2.0 88-89 5.0 90-91 2.0 92-93 3.0 94-95 0.0 96-97 10.0 98-99 4.0 100-101 8.0 102-103 2.0 104-105 2.0 106-107 8.0 108-109 9.0 110-111 8.0 112-113 9.0 114-115 3.0 116-117 6.0 118-119 3.0 120-121 1.0 122-123 13.0 124-125 12.0 126-127 10.0 128-129 17.0 130-131 21.0 132-133 12.0 134-135 7.0 136-137 5.0 138-139 19.0 140-141 20.0 142-143 34.0 144-145 24.0 146-147 55.0 148-149 51.0 150-151 3614.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 5.825 #Duplication Level Percentage of deduplicated Percentage of total 1 42.91845493562232 2.5 2 17.167381974248926 2.0 3 9.012875536480687 1.575 4 8.15450643776824 1.9 5 1.7167381974248928 0.5 6 2.575107296137339 0.8999999999999999 7 0.8583690987124464 0.35000000000000003 8 1.2875536480686696 0.6 9 2.1459227467811157 1.125 >10 9.871244635193133 12.675 >50 1.2875536480686696 5.7 >100 2.1459227467811157 28.65 >500 0.8583690987124464 41.525 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCAT 998 24.95 No Hit GCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAG 663 16.575 No Hit TGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCA 493 12.325 No Hit GGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGA 279 6.9750000000000005 No Hit ATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCATCGGC 157 3.925 No Hit GTCATATACTACAAGAGTATAACATGGCCAGTTCCAAGCAAAGCCTTTAT 114 2.85 No Hit GTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAGTCGC 103 2.5749999999999997 No Hit ATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCC 90 2.25 No Hit TATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGC 71 1.775 No Hit AAGACATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTG 67 1.675 No Hit GTCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAG 44 1.0999999999999999 No Hit AGACATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGA 41 1.0250000000000001 No Hit TATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCATCGG 37 0.9249999999999999 No Hit GACATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGAT 34 0.8500000000000001 No Hit ACGTCATATACTACAAGAGTATAACATGGCCAGTTCCAAGCAAAGCCTTT 34 0.8500000000000001 No Hit TTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCATC 30 0.75 No Hit ATGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAG 30 0.75 No Hit CCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAGT 27 0.675 No Hit TTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCATCG 23 0.575 No Hit GCCGGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAG 21 0.525 No Hit CTTCAGGAGTCGCGGTGCAGTAACGGGTCTAATTGATTCAAGCAGTAAGA 20 0.5 No Hit GGACATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGA 18 0.44999999999999996 No Hit GGCCGGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGA 18 0.44999999999999996 No Hit CGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCA 17 0.42500000000000004 No Hit CACGTATAGAATCAGTTACAAGCAAAGGATTCAGAAGAACAGGATGTACG 16 0.4 No Hit GGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGG 14 0.35000000000000003 No Hit GCTAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAG 14 0.35000000000000003 No Hit GCCCGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAG 13 0.325 No Hit ATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGG 12 0.3 No Hit GCCTTTATTAACAACATTATTGAAGCTTCAGGAGTCGCGGTGCAGTAACG 12 0.3 No Hit GTCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGA 11 0.27499999999999997 No Hit CGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGG 11 0.27499999999999997 No Hit TGACATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGA 10 0.25 No Hit GGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCA 9 0.22499999999999998 No Hit GTTACAAGCAAAGGATTCAGAAGAACAGGATGTACGAAGTGATCAACAGT 9 0.22499999999999998 No Hit CTTTATTAACAACATTATTGAAGCTTCAGGAGTCGCGGTGCAGTAACGGG 9 0.22499999999999998 No Hit ATATACTACAAGAGTATAACATGGCCAGTTCCAAGCAAAGCCTTTATTAA 9 0.22499999999999998 No Hit CGACATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGA 9 0.22499999999999998 No Hit CGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGA 8 0.2 No Hit GTATAGAATCAGTTACAAGCAAAGGATTCAGAAGAACAGGATGTACGAAG 8 0.2 No Hit CATGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCA 8 0.2 No Hit GTATAACATGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAA 7 0.17500000000000002 No Hit TTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAGTCGCG 7 0.17500000000000002 No Hit GTGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAG 6 0.15 No Hit GGCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAG 6 0.15 No Hit CCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAGTCGCGGT 6 0.15 No Hit CTGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAG 6 0.15 No Hit ATACTACAAGAGTATAACATGGCCAGTTCCAAGCAAAGCCTTTATTAACA 6 0.15 No Hit TCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGGAGTCGCGG 6 0.15 No Hit TGGCCAGTTCCAAGCAAAGCCTTTATTAACAACATTATTGAAGCTTCAGG 5 0.125 No Hit GAAGCTTCAGGAGTCGCGGTGCAGTAACGGGTCTAATTGATTCAAGCAGT 5 0.125 No Hit CGTCATATACTACAAGAGTATAACATGGCCAGTTCCAAGCAAAGCCTTTA 5 0.125 No Hit GTAACAAGAAAGCTTGTACAACGCAGGTGAACTATGACTGAGTCCTAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACAT 10 0.0071111745 143.0625 1 TGTTTAT 90 0.0 79.47917 1 GCCAGTT 125 0.0 74.392494 1 TTATTCA 200 0.0 71.53125 3 GTTTATT 190 0.0 71.53125 1 GGCCAGT 60 6.780647E-8 71.53125 1 AGTTCCA 140 0.0 71.53125 4 TATTCAC 205 0.0 69.78658 4 TTTATTC 195 0.0 69.69711 2 GTTCCAA 155 0.0 69.223785 5 TTCCAAG 155 0.0 69.223785 6 TCCAAGC 155 0.0 69.223785 7 CCAAGCA 155 0.0 69.223785 8 CAGTTCC 145 0.0 69.06466 3 CCAGTTC 135 0.0 68.88194 2 CAAGCAA 165 0.0 65.02841 9 TTCACTG 225 0.0 63.583332 6 ACTGAGG 225 0.0 63.583332 9 CACTGAG 225 0.0 63.583332 8 TCACTGA 225 0.0 63.583332 7 >>END_MODULE ERR5262789 read2 length is 84-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262789_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 84-150 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5065 37.0 37.0 37.0 37.0 37.0 2 36.3305 37.0 37.0 37.0 37.0 37.0 3 36.417 37.0 37.0 37.0 37.0 37.0 4 36.4865 37.0 37.0 37.0 37.0 37.0 5 36.526 37.0 37.0 37.0 37.0 37.0 6 36.467 37.0 37.0 37.0 37.0 37.0 7 36.4265 37.0 37.0 37.0 37.0 37.0 8 36.454 37.0 37.0 37.0 37.0 37.0 9 36.4945 37.0 37.0 37.0 37.0 37.0 10-14 36.488299999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.5171 37.0 37.0 37.0 37.0 37.0 20-24 36.4854 37.0 37.0 37.0 37.0 37.0 25-29 36.4574 37.0 37.0 37.0 37.0 37.0 30-34 36.4184 37.0 37.0 37.0 37.0 37.0 35-39 36.3932 37.0 37.0 37.0 37.0 37.0 40-44 36.3587 37.0 37.0 37.0 37.0 37.0 45-49 36.391999999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.251000000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.24759999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.232699999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.1839 37.0 37.0 37.0 37.0 37.0 70-74 36.179700000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.1625 37.0 37.0 37.0 37.0 37.0 80-84 36.1065 37.0 37.0 37.0 37.0 37.0 85-89 36.09101002010637 37.0 37.0 37.0 37.0 37.0 90-94 36.052772719575685 37.0 37.0 37.0 37.0 37.0 95-99 36.0178740316539 37.0 37.0 37.0 37.0 37.0 100-104 35.99061490471845 37.0 37.0 37.0 37.0 37.0 105-109 35.92409772016923 37.0 37.0 37.0 37.0 37.0 110-114 35.88783294928653 37.0 37.0 37.0 37.0 37.0 115-119 35.88548750077779 37.0 37.0 37.0 37.0 37.0 120-124 35.739439620506154 37.0 37.0 37.0 37.0 37.0 125-129 35.71929449307322 37.0 37.0 37.0 37.0 37.0 130-134 35.79584410139563 37.0 37.0 37.0 37.0 37.0 135-139 35.64766962934881 37.0 37.0 37.0 37.0 37.0 140-144 35.583224973074095 37.0 37.0 37.0 37.0 37.0 145-149 35.67042998965974 37.0 37.0 37.0 37.0 37.0 150 35.62049861495845 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 0.0 14 0.0 15 1.0 16 0.0 17 0.0 18 0.0 19 2.0 20 3.0 21 4.0 22 7.0 23 12.0 24 4.0 25 4.0 26 3.0 27 4.0 28 7.0 29 7.0 30 12.0 31 27.0 32 40.0 33 71.0 34 169.0 35 419.0 36 2827.0 37 374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.949999999999996 23.549999999999997 5.925 29.575000000000003 2 25.0 25.8 21.9 27.3 3 22.45 24.975 19.6 32.975 4 24.725 34.050000000000004 14.899999999999999 26.325 5 24.7 35.025 14.85 25.424999999999997 6 25.25 38.3 14.7 21.75 7 22.400000000000002 21.95 28.9 26.75 8 24.474999999999998 24.7 17.0 33.825 9 21.975 24.65 22.475 30.9 10-14 26.075 27.35 18.295 28.28 15-19 24.18 28.68 18.115000000000002 29.025000000000002 20-24 23.84 29.04 19.23 27.889999999999997 25-29 25.430000000000003 27.834999999999997 20.26 26.474999999999998 30-34 24.125 28.395 20.075000000000003 27.405 35-39 23.665 28.134999999999998 20.1 28.1 40-44 22.384999999999998 27.99 23.04 26.584999999999997 45-49 24.675 27.265 22.009999999999998 26.05 50-54 23.294999999999998 28.610000000000003 23.79 24.305 55-59 22.55 28.58 22.955000000000002 25.915 60-64 22.79 26.22 23.64 27.35 65-69 22.875 25.724999999999998 25.36 26.040000000000003 70-74 24.625 25.240000000000002 24.47 25.665 75-79 22.205 27.644999999999996 22.475 27.675 80-84 21.17 26.26 24.945 27.625 85-89 22.306730047535652 25.16387290467851 25.74430823117338 26.785088816612458 90-94 22.271793072334454 24.03127976339666 26.29705749661637 27.399869667652517 95-99 22.01687424668542 27.209722780233026 24.92969063881077 25.84371233427079 100-104 22.378503730590847 25.67049808429119 25.15628150836862 26.794716676749346 105-109 23.648682813369064 24.836931789452393 24.68018405218183 26.83420134499671 110-114 23.46187064979932 24.62023065589595 24.467814865620078 27.45008382868465 115-119 25.698694410444716 22.322521419828643 26.739086087311303 25.23969808241534 120-124 24.49491074625339 23.78906449797964 25.66620633215692 26.049818423610045 125-129 24.17576756645374 24.093344323099114 27.560271996703072 24.170616113744074 130-134 25.512753774076003 22.727745965642896 27.881311816762107 23.878188443519 135-139 24.51950772453522 25.697826656192717 25.69258968316313 24.090075936108928 140-144 24.111594356635198 26.328630529330642 27.103002015487434 22.45677309854673 145-149 23.610734616427216 26.42450528598536 26.33233938736785 23.63242071021957 150 26.980609418282548 24.210526315789473 26.177285318559555 22.63157894736842 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 1.0 28 3.0 29 4.0 30 2.5 31 0.5 32 1.0 33 1.5 34 1.5 35 1.5 36 2.0 37 3.5 38 3.5 39 4.0 40 7.5 41 9.0 42 33.5 43 159.5 44 288.5 45 349.0 46 287.5 47 276.0 48 362.5 49 331.5 50 280.0 51 219.0 52 136.0 53 60.0 54 75.5 55 82.5 56 31.0 57 60.0 58 163.0 59 182.5 60 93.0 61 47.0 62 47.0 63 52.5 64 78.0 65 89.0 66 74.5 67 50.5 68 17.0 69 5.0 70 2.5 71 2.5 72 2.0 73 1.0 74 2.0 75 2.5 76 2.0 77 2.0 78 1.5 79 1.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 84-85 1.0 86-87 2.0 88-89 5.0 90-91 2.0 92-93 3.0 94-95 0.0 96-97 10.0 98-99 4.0 100-101 8.0 102-103 2.0 104-105 2.0 106-107 8.0 108-109 9.0 110-111 8.0 112-113 9.0 114-115 3.0 116-117 6.0 118-119 3.0 120-121 2.0 122-123 13.0 124-125 12.0 126-127 10.0 128-129 17.0 130-131 21.0 132-133 12.0 134-135 7.0 136-137 5.0 138-139 19.0 140-141 21.0 142-143 34.0 144-145 26.0 146-147 55.0 148-149 51.0 150-151 3610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.025 #Duplication Level Percentage of deduplicated Percentage of total 1 36.662286465177395 6.9750000000000005 2 14.586070959264127 5.55 3 9.85545335085414 5.625 4 5.913272010512484 4.5 5 5.387647831800263 5.125 6 3.942181340341656 4.5 7 3.942181340341656 5.25 8 1.8396846254927726 2.8000000000000003 9 3.28515111695138 5.625 >10 14.191852825229962 49.1 >50 0.39421813403416556 4.95 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GACCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCA 68 1.7000000000000002 No Hit GTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGA 68 1.7000000000000002 No Hit CTTAAAAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCG 62 1.55 No Hit GGCCCAACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGC 43 1.075 No Hit ACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAGATCTCAGA 42 1.05 No Hit ATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATAT 40 1.0 No Hit GGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATCG 40 1.0 No Hit CTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAAATTGC 37 0.9249999999999999 No Hit CCCAACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCT 37 0.9249999999999999 No Hit GCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCAC 36 0.8999999999999999 No Hit ACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTTGT 34 0.8500000000000001 No Hit GGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACC 33 0.8250000000000001 No Hit GACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAG 31 0.775 No Hit CCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAGAT 31 0.775 No Hit AGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACCTGCGGTCGC 29 0.7250000000000001 No Hit ACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAG 29 0.7250000000000001 No Hit CGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTGCTTG 29 0.7250000000000001 No Hit GCCCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGAT 28 0.7000000000000001 No Hit GCAAGCCCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACG 28 0.7000000000000001 No Hit ATCAAATGGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGT 28 0.7000000000000001 No Hit GAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCAT 27 0.675 No Hit CACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTT 26 0.65 No Hit GGGAATCCTTGCAGAGGTGAATGCCGTGGGGTATTGGCCGAAGTGCTGCG 26 0.65 No Hit GTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAACATCAAATGGA 26 0.65 No Hit GGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACGATGAAGA 25 0.625 No Hit CTTCGGAGACAACATCAAATGGAACTTCTCCAAGTTTTTGATTGACAAGG 25 0.625 No Hit GCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGTTTTTGATT 25 0.625 No Hit ATCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAAATT 25 0.625 No Hit CGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAA 24 0.6 No Hit GCCCAACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCC 23 0.575 No Hit GGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGATCGC 23 0.575 No Hit GCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCT 22 0.5499999999999999 No Hit CAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAA 21 0.525 No Hit GCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTTGTTACTGCT 21 0.525 No Hit GATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAAATTGCCATC 21 0.525 No Hit ATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTTGTTACTG 20 0.5 No Hit CCCCAACTGCTTGGCCTGCCGCGTGGTGCAGGAGAAGCCCGTCAAGACGT 20 0.5 No Hit CACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAA 20 0.5 No Hit GAGAAGTACAAGGACCAGGGTTTCGAGATCCTTGCTTTCCCATGCAACCA 19 0.475 No Hit GCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACCT 19 0.475 No Hit CGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAGATC 19 0.475 No Hit AAGGCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGTTTTTG 18 0.44999999999999996 No Hit GCTTGGCCTGCCGCGTGGTGCAGGAGAAGCCCGTCAAGACGTTCCGGTGC 18 0.44999999999999996 No Hit GTCGCAAGCCCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCA 18 0.44999999999999996 No Hit GATTGACAAGGAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCC 18 0.44999999999999996 No Hit CAACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTT 17 0.42500000000000004 No Hit AGCAAAGGCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGTT 17 0.42500000000000004 No Hit GCGTGGTGCAGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTC 17 0.42500000000000004 No Hit GCCGAGTATCCAATTTTCGACAAGGTTGATGTTAATGGTGAGAATGTCGC 16 0.4 No Hit AGATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAAATTGCCAT 16 0.4 No Hit CCGACCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCAC 16 0.4 No Hit CCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAA 16 0.4 No Hit GAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTTGTTA 15 0.375 No Hit AACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGA 15 0.375 No Hit AGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCA 15 0.375 No Hit CAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAGATCTCAGATG 15 0.375 No Hit AAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAAT 15 0.375 No Hit AAAAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAA 15 0.375 No Hit GTCCAGCAAAGGCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCA 15 0.375 No Hit AACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGATCGCTA 14 0.35000000000000003 No Hit GGCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGTTTTTGAT 14 0.35000000000000003 No Hit GATGGCCCAACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGT 14 0.35000000000000003 No Hit CAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAAATTGCCA 14 0.35000000000000003 No Hit ATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGAT 14 0.35000000000000003 No Hit GCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCT 14 0.35000000000000003 No Hit GCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGA 14 0.35000000000000003 No Hit CATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTGC 14 0.35000000000000003 No Hit GTCGCACCCATCTACAAGTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGG 14 0.35000000000000003 No Hit CCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAGATCTCAGATGAGAT 14 0.35000000000000003 No Hit CCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAG 13 0.325 No Hit GCGCCGACCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAG 13 0.325 No Hit GTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCT 13 0.325 No Hit GGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACGATGAAGAGCAGCA 13 0.325 No Hit GGAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCA 13 0.325 No Hit ATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTGCT 13 0.325 No Hit GATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATA 13 0.325 No Hit CAACATCAAATGGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATG 13 0.325 No Hit ACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCT 12 0.3 No Hit CTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCA 12 0.3 No Hit CTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAACATCAAATGGAACTT 12 0.3 No Hit AGATCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAAA 12 0.3 No Hit AGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTGCTTGAATCA 12 0.3 No Hit ACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACGATGA 12 0.3 No Hit CTACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGC 12 0.3 No Hit TGTTAATGGTGAGAATGTCGCACCCATCTACAAGTTTCTGAAGTCCAGCA 12 0.3 No Hit GTGAGAATGTCGCACCCATCTACAAGTTTCTGAAGTCCAGCAAAGGCAGC 12 0.3 No Hit GCTGCGACAACTGCAGGACCTTCTCGGGGGTCATGGTCTGCGACGACGCC 12 0.3 No Hit AGCCCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGA 12 0.3 No Hit GATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTTGTTACT 12 0.3 No Hit CCTAGTTCTCCAGGCCATCCTGGTCATGGGAATCCTTGCAGAGGTGAATG 12 0.3 No Hit GAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCA 12 0.3 No Hit CCAACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTT 12 0.3 No Hit GACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACCTGCGGTCGCA 11 0.27499999999999997 No Hit CACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTT 11 0.27499999999999997 No Hit ATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAAG 11 0.27499999999999997 No Hit AAATGGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGA 11 0.27499999999999997 No Hit CGGAACTGAGCCAGTTGTATGAGAAGTACAAGGACCAGGGTTTCGAGATC 11 0.27499999999999997 No Hit GTGCAGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGA 11 0.27499999999999997 No Hit CGCAAGCCCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAAC 10 0.25 No Hit CCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACGATGAA 10 0.25 No Hit CACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAG 10 0.25 No Hit GCAGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAG 10 0.25 No Hit CAAGGAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTA 10 0.25 No Hit GTTCAGTTTGCTTGCACTCGCTTCAAAGCCGAGTATCCAATTTTCGACAA 10 0.25 No Hit GCGACAACTGCAGGACCTTCTCGGGGGTCATGGTCTGCGACGACGCCATG 10 0.25 No Hit CAAGCCCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGA 10 0.25 No Hit CTGGCACTAATGAGGAGATTGTTCAGTTTGCTTGCACTCGCTTCAAAGCC 10 0.25 No Hit CAAAGGCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGTTTT 10 0.25 No Hit CCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGC 10 0.25 No Hit CCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACT 10 0.25 No Hit GCAAAGGCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGTTT 10 0.25 No Hit AGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAAC 9 0.22499999999999998 No Hit CGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACC 9 0.22499999999999998 No Hit GTTGTATGAGAAGTACAAGGACCAGGGTTTCGAGATCCTTGCTTTCCCAT 9 0.22499999999999998 No Hit GCCGAAGTGCTGCGACAACTGCAGGACCTTCTCGGGGGTCATGGTCTGCG 9 0.22499999999999998 No Hit AATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGA 9 0.22499999999999998 No Hit GGAACCTGGCACTAATGAGGAGATTGTTCAGTTTGCTTGCACTCGCTTCA 9 0.22499999999999998 No Hit TCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATATATAAATTG 9 0.22499999999999998 No Hit TGGGAATCCTTGCAGAGGTGAATGCCGTGGGGTATTGGCCGAAGTGCTGC 9 0.22499999999999998 No Hit AAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACC 9 0.22499999999999998 No Hit TGGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGATCG 9 0.22499999999999998 No Hit AAGCCCTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAG 9 0.22499999999999998 No Hit ATCCAATTTTCGACAAGGTTGATGTTAATGGTGAGAATGTCGCACCCATC 9 0.22499999999999998 No Hit ACCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCAC 9 0.22499999999999998 No Hit GTCATGGGAATCCTTGCAGAGGTGAATGCCGTGGGGTATTGGCCGAAGTG 9 0.22499999999999998 No Hit ACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTC 9 0.22499999999999998 No Hit GCACGCTCACGGCGATCCTAGTTCTCCAGGCCATCCTGGTCATGGGAATC 9 0.22499999999999998 No Hit AGACAACATCAAATGGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGAC 9 0.22499999999999998 No Hit CACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCACA 9 0.22499999999999998 No Hit CGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACGATGAAG 9 0.22499999999999998 No Hit CGACCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACC 9 0.22499999999999998 No Hit AGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGAC 9 0.22499999999999998 No Hit GTTTGGTGGGCAGGAACCTGGCACTAATGAGGAGATTGTTCAGTTTGCTT 9 0.22499999999999998 No Hit ACAAGTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAACATCAAA 9 0.22499999999999998 No Hit GCCAAGTGCCACCCCAACTGCTTGGCCTGCCGCGTGGTGCAGGAGAAGCC 9 0.22499999999999998 No Hit GGCCAAGTGCCACCCCAACTGCTTGGCCTGCCGCGTGGTGCAGGAGAAGC 9 0.22499999999999998 No Hit CAAGTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAACATCAAAT 8 0.2 No Hit TAAAAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGA 8 0.2 No Hit GTTGATGTTAATGGTGAGAATGTCGCACCCATCTACAAGTTTCTGAAGTC 8 0.2 No Hit TCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCA 8 0.2 No Hit GACAACTGCAGGACCTTCTCGGGGGTCATGGTCTGCGACGACGCCATGGC 8 0.2 No Hit GGTGCGCCGACCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGC 8 0.2 No Hit AAAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAA 8 0.2 No Hit TACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCT 8 0.2 No Hit CTACAAGTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAACATCA 8 0.2 No Hit ATGGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGATC 8 0.2 No Hit GTCTGCGACGACGCCATGGCCAAGTGCCACCCCAACTGCTTGGCCTGCCG 8 0.2 No Hit CCTGGCACTAATGAGGAGATTGTTCAGTTTGCTTGCACTCGCTTCAAAGC 8 0.2 No Hit GTTAATGGTGAGAATGTCGCACCCATCTACAAGTTTCTGAAGTCCAGCAA 8 0.2 No Hit GAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTGCTTGA 8 0.2 No Hit GGTATTGGCCGAAGTGCTGCGACAACTGCAGGACCTTCTCGGGGGTCATG 7 0.17500000000000002 No Hit CACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATC 7 0.17500000000000002 No Hit GGGAAAGATGTGGACCTGAGCACCTACAAGGGGAAAGTTCTCCTCATCGT 7 0.17500000000000002 No Hit AAGAGCAGCACGCTCACGGCGATCCTAGTTCTCCAGGCCATCCTGGTCAT 7 0.17500000000000002 No Hit GAGCAGCACGCTCACGGCGATCCTAGTTCTCCAGGCCATCCTGGTCATGG 7 0.17500000000000002 No Hit GATCCTTGCTTTCCCATGCAACCAGTTTGGTGGGCAGGAACCTGGCACTA 7 0.17500000000000002 No Hit CTGCGACGACGCCATGGCCAAGTGCCACCCCAACTGCTTGGCCTGCCGCG 7 0.17500000000000002 No Hit CAGCAAAGGCAGCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGT 7 0.17500000000000002 No Hit CCCATCTACAAGTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAA 7 0.17500000000000002 No Hit CCCAACTGCTTGGCCTGCCGCGTGGTGCAGGAGAAGCCCGTCAAGACGTT 7 0.17500000000000002 No Hit AAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAA 7 0.17500000000000002 No Hit CCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACCTG 7 0.17500000000000002 No Hit GGAGATTGTTCAGTTTGCTTGCACTCGCTTCAAAGCCGAGTATCCAATTT 7 0.17500000000000002 No Hit GGGGTATTGGCCGAAGTGCTGCGACAACTGCAGGACCTTCTCGGGGGTCA 7 0.17500000000000002 No Hit AACTGCTTGGCCTGCCGCGTGGTGCAGGAGAAGCCCGTCAAGACGTTCCG 7 0.17500000000000002 No Hit CTAAGAAGCAACGATGAAGAGCAGCACGCTCACGGCGATCCTAGTTCTCC 7 0.17500000000000002 No Hit AGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACC 7 0.17500000000000002 No Hit GGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATTG 7 0.17500000000000002 No Hit CATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAA 7 0.17500000000000002 No Hit CAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGATCGCTACGCACCAA 7 0.17500000000000002 No Hit CCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAGATCTCAGAT 7 0.17500000000000002 No Hit GAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAA 7 0.17500000000000002 No Hit GGTGCAGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCG 7 0.17500000000000002 No Hit GTGAATGCCGTGGGGTATTGGCCGAAGTGCTGCGACAACTGCAGGACCTT 7 0.17500000000000002 No Hit AGGAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGC 7 0.17500000000000002 No Hit GTGCCACCCCAACTGCTTGGCCTGCCGCGTGGTGCAGGAGAAGCCCGTCA 7 0.17500000000000002 No Hit GAGATGGCCCAACGAGATGCAGACCACAAAATATATAAATTGCCATCATC 7 0.17500000000000002 No Hit GTTCTCCTCATCGTCAATGTTGCTTCCCAGTGTGGCCTGACCAATTCCAA 7 0.17500000000000002 No Hit CATGGCCAAGTGCCACCCCAACTGCTTGGCCTGCCGCGTGGTGCAGGAGA 7 0.17500000000000002 No Hit CAAATGGAACTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGG 7 0.17500000000000002 No Hit CTTCTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGATCGCTACG 6 0.15 No Hit CAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACCTGCGGTC 6 0.15 No Hit CTCGTAGATTTGCCTGCACAGGTTTCTCCGATCCGCCCAGTTTTTGTTGT 6 0.15 No Hit CAGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGA 6 0.15 No Hit CTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCT 6 0.15 No Hit GTATTGGCCGAAGTGCTGCGACAACTGCAGGACCTTCTCGGGGGTCATGG 6 0.15 No Hit CGGAGACAACATCAAATGGAACTTCTCCAAGTTTTTGATTGACAAGGAGG 6 0.15 No Hit ATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAG 6 0.15 No Hit AGGGTTTCGAGATCCTTGCTTTCCCATGCAACCAGTTTGGTGGGCAGGAA 6 0.15 No Hit GCAGCACGCTCACGGCGATCCTAGTTCTCCAGGCCATCCTGGTCATGGGA 6 0.15 No Hit GTTTTTGATTGACAAGGAGGGACATGTCGTGGATCGCTACGCACCAACCA 6 0.15 No Hit AACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTTG 6 0.15 No Hit GAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTGCTTGAAT 6 0.15 No Hit TGATCAGATCTCAGATGAGATGGCCCAACGAGATGCAGACCACAAAATAT 6 0.15 No Hit GACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACGATG 6 0.15 No Hit CGGAAATCTAAGAAGCAACGATGAAGAGCAGCACGCTCACGGCGATCCTA 6 0.15 No Hit CGACAAGGTTGATGTTAATGGTGAGAATGTCGCACCCATCTACAAGTTTC 6 0.15 No Hit TCGGAGACAACATCAAATGGAACTTCTCCAAGTTTTTGATTGACAAGGAG 6 0.15 No Hit GCAGGACCTTCTCGGGGGTCATGGTCTGCGACGACGCCATGGCCAAGTGC 6 0.15 No Hit GTTTCGAGATCCTTGCTTTCCCATGCAACCAGTTTGGTGGGCAGGAACCT 6 0.15 No Hit GCCGCGTGGTGCAGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGACCAA 6 0.15 No Hit TTAGCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTA 6 0.15 No Hit ACTGCTTGGCCTGCCGCGTGGTGCAGGAGAAGCCCGTCAAGACGTTCCGG 6 0.15 No Hit GGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTGCTTGAATCAA 6 0.15 No Hit CATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTA 6 0.15 No Hit CCTGCCGCGTGGTGCAGGAGAAGCCCGTCAAGACGTTCCGGTGCGCCGAC 6 0.15 No Hit GCATCTTCGGAGACAACATCAAATGGAACTTCTCCAAGTTTTTGATTGAC 6 0.15 No Hit ATGGCCCAACGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTC 6 0.15 No Hit CGGCGATCCTAGTTCTCCAGGCCATCCTGGTCATGGGAATCCTTGCAGAG 6 0.15 No Hit AGCATGGCCGCCGCCGCCTCCTCCGCCTCTTCCGTCCACGACTTCACCGT 6 0.15 No Hit CTACAACTTAAAAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTT 5 0.125 No Hit AGTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAACATCAAATGG 5 0.125 No Hit CGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAAGAAGCTGC 5 0.125 No Hit CTCCAAGTTTTTGATTGACAAGGAGGGACATGTCGTGGATCGCTACGCAC 5 0.125 No Hit GTTCTCCAGGCCATCCTGGTCATGGGAATCCTTGCAGAGGTGAATGCCGT 5 0.125 No Hit GGTGAGAATGTCGCACCCATCTACAAGTTTCTGAAGTCCAGCAAAGGCAG 5 0.125 No Hit GTTTGCCATAGGCCTGCCACAGTATTTAGTTCAAGGGGCTGATTCTAGGT 5 0.125 No Hit CCTGACCAATTCCAATTACACGGAACTGAGCCAGTTGTATGAGAAGTACA 5 0.125 No Hit ATTGACAAGGAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCC 5 0.125 No Hit GCCTGACCAATTCCAATTACACGGAACTGAGCCAGTTGTATGAGAAGTAC 5 0.125 No Hit GATGAAGAGCAGCACGCTCACGGCGATCCTAGTTCTCCAGGCCATCCTGG 5 0.125 No Hit CAACTGCAGGACCTTCTCGGGGGTCATGGTCTGCGACGACGCCATGGCCA 5 0.125 No Hit AGTGCTGCGACAACTGCAGGACCTTCTCGGGGGTCATGGTCTGCGACGAC 5 0.125 No Hit GCCGACCAATTCACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCA 5 0.125 No Hit CGTCAAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACCTGCG 5 0.125 No Hit AAGACGTTCCGGTGCGCCGACCAATTCACCGAAGACCAAACCTGCGGTCG 5 0.125 No Hit TCCAATTACACGGAACTGAGCCAGTTGTATGAGAAGTACAAGGACCAGGG 5 0.125 No Hit ACCGAAGACCAAACCTGCGGTCGCAAGCCCTGCAAGCACCACTGATCAGA 5 0.125 No Hit ACAAGGAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTT 5 0.125 No Hit ACAACTTAAAAGCATTCCCCAAGTAGACCGGACAAGGCGCGGCAGCTTTG 5 0.125 No Hit GGCCTGACCAATTCCAATTACACGGAACTGAGCCAGTTGTATGAGAAGTA 5 0.125 No Hit TGATTGACAAGGAGGGACATGTCGTGGATCGCTACGCACCAACCACTTCC 5 0.125 No Hit AGGGACATGTCGTGGATCGCTACGCACCAACCACTTCCCCACTTAGCATC 5 0.125 No Hit CAATTTTCGACAAGGTTGATGTTAATGGTGAGAATGTCGCACCCATCTAC 5 0.125 No Hit CAAGTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCA 5 0.125 No Hit CGAGTATCCAATTTTCGACAAGGTTGATGTTAATGGTGAGAATGTCGCAC 5 0.125 No Hit ACCAGTTTGGTGGGCAGGAACCTGGCACTAATGAGGAGATTGTTCAGTTT 5 0.125 No Hit TGGCCGAAGTGCTGCGACAACTGCAGGACCTTCTCGGGGGTCATGGTCTG 5 0.125 No Hit ATAGGCCTGCCACAGTATTTAGTTCAAGGGGCTGATTCTAGGTCCAACAG 5 0.125 No Hit GCCGTGGGGTATTGGCCGAAGTGCTGCGACAACTGCAGGACCTTCTCGGG 5 0.125 No Hit TATTGGCCGAAGTGCTGCGACAACTGCAGGACCTTCTCGGGGGTCATGGT 5 0.125 No Hit TACAAGTTTCTGAAGTCCAGCAAAGGCAGCATCTTCGGAGACAACATCAA 5 0.125 No Hit CGAGATGCAGACCACAAAATATATAAATTGCCATCATCGTCGCCTTTGTT 5 0.125 No Hit GCATCGAGAAGGACCTGAAGAAGCTGCTTGCGAGTTCTTAAATCTTACTG 5 0.125 No Hit GAACTGAGCCAGTTGTATGAGAAGTACAAGGACCAGGGTTTCGAGATCCT 5 0.125 No Hit AGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACGATGAAGAGCAGC 5 0.125 No Hit GTTGCTTCCCAGTGTGGCCTGACCAATTCCAATTACACGGAACTGAGCCA 5 0.125 No Hit AGTACAAGGACCAGGGTTTCGAGATCCTTGCTTTCCCATGCAACCAGTTT 5 0.125 No Hit GATCGCTACGCACCAACCACTTCCCCACTTAGCATCGAGAAGGACCTGAA 5 0.125 No Hit TTTTCGACAAGGTTGATGTTAATGGTGAGAATGTCGCACCCATCTACAAG 5 0.125 No Hit GGTTGATGTTAATGGTGAGAATGTCGCACCCATCTACAAGTTTCTGAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGACC 35 0.0038233707 20.4375 15-19 CCGGACA 35 0.0038233707 20.4375 20-24 CCCAAGT 35 0.0038233707 20.4375 9 CGCGGCA 35 0.0038233707 20.4375 30-34 CAAGGCG 35 0.0038233707 20.4375 25-29 ATCTAAG 40 0.0082724895 17.882812 50-54 CGGAAAT 40 0.0082724895 17.882812 45-49 TTGCGCG 40 0.0082724895 17.882812 6 CAGCTTT 40 0.0082724895 17.882812 1 CAACGAT 40 0.0082724895 17.882812 60-64 >>END_MODULE Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393688 spots for ERR5262789.sra Written 1393688 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra Read 1393670 spots for ERR5262789.sra Written 1393670 spots for ERR5262789.sra SRR ids: ['ERR5262789.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_yqttbj7_ ERR5262789.sra spots: 27873418 blocks: [[1, 1393670], [1393671, 2787340], [2787341, 4181010], [4181011, 5574680], [5574681, 6968350], [6968351, 8362020], [8362021, 9755690], [9755691, 11149360], [11149361, 12543030], [12543031, 13936700], [13936701, 15330370], [15330371, 16724040], [16724041, 18117710], [18117711, 19511380], [19511381, 20905050], [20905051, 22298720], [22298721, 23692390], [23692391, 25086060], [25086061, 26479730], [26479731, 27873418]] ERR5262789 file size 9066776 ERR5262789 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262789 ERR5262789_1.fastq ERR5262789_2.fastq Input file: ERR5262789_1.fastq Paired file: ERR5262789_2.fastq trimmed: ERR5262789-trimmed-pair1.fastq, ERR5262789-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:38:19 2024 >> started Fri Dec 6 11:38:56 2024 >> done (36.653s) 27873418 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 27873418 (100.00%) read pairs available; of these: 9047 ( 0.03%) trimmed read pairs available after processing 27864371 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 1 0.00% 22 1 0.00% 23 2 0.00% 24 1 0.00% 25 1 0.00% 26 1 0.00% 27 2 0.00% 28 0 0.00% 29 2 0.00% 30 2 0.00% 31 2 0.00% 32 3 0.00% 33 2 0.00% 34 1 0.00% 35 2 0.00% 36 2 0.00% 37 1 0.00% 38 3 0.00% 39 1 0.00% 40 0 0.00% 41 2 0.00% 42 1 0.00% 43 1 0.00% 44 2 0.00% 45 1 0.00% 46 0 0.00% 47 3 0.00% 48 5 0.00% 49 493 0.00% 50 567 0.00% 51 654 0.00% 52 694 0.00% 53 758 0.00% 54 797 0.00% 55 925 0.00% 56 998 0.00% 57 1093 0.00% 58 1186 0.00% 59 1489 0.01% 60 1586 0.01% 61 1896 0.01% 62 2151 0.01% 63 2362 0.01% 64 2480 0.01% 65 2762 0.01% 66 2930 0.01% 67 3424 0.01% 68 3865 0.01% 69 4346 0.02% 70 4893 0.02% 71 5557 0.02% 72 6573 0.02% 73 7317 0.03% 74 7929 0.03% 75 8907 0.03% 76 9805 0.04% 77 10434 0.04% 78 11454 0.04% 79 12825 0.05% 80 13962 0.05% 81 16033 0.06% 82 17890 0.06% 83 19916 0.07% 84 21654 0.08% 85 23178 0.08% 86 24990 0.09% 87 26129 0.09% 88 28163 0.10% 89 29469 0.11% 90 32047 0.11% 91 34346 0.12% 92 36599 0.13% 93 39273 0.14% 94 42467 0.15% 95 43920 0.16% 96 46500 0.17% 97 48273 0.17% 98 49346 0.18% 99 51293 0.18% 100 53425 0.19% 101 55632 0.20% 102 58128 0.21% 103 60999 0.22% 104 62542 0.22% 105 65585 0.24% 106 67397 0.24% 107 69552 0.25% 108 69976 0.25% 109 73022 0.26% 110 73373 0.26% 111 75497 0.27% 112 78059 0.28% 113 80909 0.29% 114 82731 0.30% 115 84928 0.30% 116 87192 0.31% 117 88846 0.32% 118 89545 0.32% 119 89937 0.32% 120 91072 0.33% 121 93481 0.34% 122 94725 0.34% 123 96518 0.35% 124 99305 0.36% 125 101079 0.36% 126 101324 0.36% 127 103515 0.37% 128 103435 0.37% 129 105102 0.38% 130 105660 0.38% 131 105539 0.38% 132 108639 0.39% 133 109697 0.39% 134 110674 0.40% 135 113164 0.41% 136 113899 0.41% 137 114970 0.41% 138 115701 0.42% 139 117807 0.42% 140 118303 0.42% 141 121661 0.44% 142 124162 0.45% 143 124396 0.45% 144 125956 0.45% 145 124775 0.45% 146 128363 0.46% 147 242317 0.87% 148 120341 0.43% 149 119822 0.43% 150 22182097 79.58% 27873418 reads passed initial QC criterion=sequence-density sequence-density=2.39 sequence-density-rank=1 fanout-score=3.18 fanout-score-rank=25 prefix-density=2.98 prefix-fanout=2.5 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=335.61 fanout-score-rank=1 prefix-density=0.82 prefix-fanout=8.3 sequence=TTCTTCTTCTGCTCCGGGGTGAACTCCGGCAGCCGTGATCCCACGAGGCCGCGCATGGTGGCCGGGTACTCGCCGAACGTCACGGGGTGCTGGAACCAGCCCAACATGAAGTCCAAGCTGCGCTCCTGCGCGCGCACGTCGGCCAAGGATTTCGGGTCGTAGGGCTCGAACCAGTTGGACACCTGCGTGATCCCGATCTTGCCGCCTTGGGTCTTTTGGTACTTTGTCCTGTAGAGCTCCACGGCCTCGGCGTGTGCGAGGAGAAGGTTGTGGCCTGCGATGTAAGGCTCTGTAGCTGAGTTTCCGGCGCCGCAGGTCTTGGAGACGTATGGGGAGCAGCGGCCCGGGGCGGCGATGCCCGTGGCGTAGCCGCCCGAGCAGAAGATCATTGGCTCGTTGAAGGTGTTCCAGAGCTTGATTCGGTCCCCGAATAGCCCGAACACCAGGTCCGCGTACTCCACGTAGTCCTTGATGATCTT criterion=sequence-density sequence-density=8.80 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=31 prefix-density=8.77 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=46.11 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=1.0 sequence=CGCTACTTTCTACTAGTGAAGCTCAATCCACAGCCACAAGTAACACAATGGCTTCCGGTCTTAAGATTGCCGTGGTTGTTGCCGTCTGTGCCATGCTGCTAATCCTGAACGCAAGCCCGGCCGCGGCACAGCGGCAGGTAGAATGCAAGAATTGCGGCACGTTCTGCGGCACTGCGTGCAGTGATTTGCGTGCAG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262789 ERR5262789_1.fastq ERR5262789_2.fastq Input file: ERR5262789_1.fastq Paired file: ERR5262789_2.fastq trimmed: ERR5262789-trimmed-pair1.fastq, ERR5262789-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:42:28 2024 >> started Fri Dec 6 11:42:47 2024 >> done (18.973s) 18582279 read pairs processed; of these: 111 ( 0.00%) short read pairs filtered out after trimming by size control 290 ( 0.00%) empty read pairs filtered out after trimming by size control 18581878 (100.00%) read pairs available; of these: 182 ( 0.00%) trimmed read pairs available after processing 18581696 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 1 0.00% 22 1 0.00% 23 2 0.00% 24 1 0.00% 25 1 0.00% 26 0 0.00% 27 1 0.00% 28 0 0.00% 29 0 0.00% 30 1 0.00% 31 0 0.00% 32 3 0.00% 33 1 0.00% 34 1 0.00% 35 1 0.00% 36 1 0.00% 37 1 0.00% 38 2 0.00% 39 1 0.00% 40 0 0.00% 41 0 0.00% 42 1 0.00% 43 1 0.00% 44 1 0.00% 45 0 0.00% 46 0 0.00% 47 2 0.00% 48 3 0.00% 49 413 0.00% 50 478 0.00% 51 547 0.00% 52 334 0.00% 53 600 0.00% 54 675 0.00% 55 797 0.00% 56 843 0.00% 57 905 0.00% 58 1017 0.01% 59 720 0.00% 60 476 0.00% 61 1356 0.01% 62 1881 0.01% 63 1819 0.01% 64 738 0.00% 65 2349 0.01% 66 976 0.01% 67 2845 0.02% 68 2429 0.01% 69 3090 0.02% 70 2877 0.02% 71 3307 0.02% 72 4958 0.03% 73 4824 0.03% 74 5088 0.03% 75 6028 0.03% 76 6729 0.04% 77 5938 0.03% 78 7485 0.04% 79 8374 0.05% 80 8216 0.04% 81 10136 0.05% 82 11754 0.06% 83 13238 0.07% 84 14890 0.08% 85 15529 0.08% 86 14783 0.08% 87 17305 0.09% 88 19063 0.10% 89 20054 0.11% 90 21288 0.11% 91 22750 0.12% 92 25094 0.14% 93 25120 0.14% 94 26872 0.14% 95 29681 0.16% 96 31685 0.17% 97 31979 0.17% 98 31503 0.17% 99 34348 0.18% 100 36298 0.20% 101 37080 0.20% 102 39882 0.21% 103 39703 0.21% 104 41786 0.22% 105 42133 0.23% 106 45997 0.25% 107 46117 0.25% 108 47900 0.26% 109 49265 0.27% 110 48594 0.26% 111 51245 0.28% 112 51427 0.28% 113 55017 0.30% 114 55278 0.30% 115 56610 0.30% 116 58565 0.32% 117 58495 0.31% 118 59671 0.32% 119 60604 0.33% 120 60658 0.33% 121 62944 0.34% 122 63689 0.34% 123 64616 0.35% 124 66145 0.36% 125 67193 0.36% 126 67649 0.36% 127 68767 0.37% 128 69180 0.37% 129 70165 0.38% 130 70269 0.38% 131 70517 0.38% 132 72785 0.39% 133 73163 0.39% 134 74031 0.40% 135 74859 0.40% 136 76104 0.41% 137 76910 0.41% 138 77202 0.42% 139 78244 0.42% 140 78405 0.42% 141 80383 0.43% 142 82956 0.45% 143 83063 0.45% 144 83641 0.45% 145 82814 0.45% 146 86082 0.46% 147 161827 0.87% 148 80358 0.43% 149 79604 0.43% 150 14789776 79.59% criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=4.48 fanout-score-rank=29 prefix-density=0.37 prefix-fanout=3.2 sequence=GATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=fanout-score sequence-density=0.05 sequence-density-rank=28 fanout-score=204.92 fanout-score-rank=1 prefix-density=0.77 prefix-fanout=14.0 sequence=CGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGA criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=34 prefix-density=0.69 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.13 sequence-density-rank=18 fanout-score=175.28 fanout-score-rank=1 prefix-density=1.06 prefix-fanout=21.3 sequence=CGCCGCCGCCGC ERR5262789 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:43:41 Started mapping on | Dec 06 11:43:41 Finished on | Dec 06 11:46:11 Mapping speed, Million of reads per hour | 668.95 Number of input reads | 27873017 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 26929914 Uniquely mapped reads % | 96.62% Average mapped length | 287.98 Number of splices: Total | 23426429 Number of splices: Annotated (sjdb) | 21814219 Number of splices: GT/AG | 23101366 Number of splices: GC/AG | 269565 Number of splices: AT/AC | 14205 Number of splices: Non-canonical | 41293 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.26 Insertion rate per base | 0.01% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 290607 % of reads mapped to multiple loci | 1.04% Number of reads mapped to too many loci | 944 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.33% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 652496 652496 652496 N_multimapping 290607 290607 290607 N_noFeature 902934 26196182 1128520 N_ambiguous 590758 3207 83170 UnstrandedReadsAssigned:25436222 PositiveStrandReadsAssigned:730525 NegativeStrandReadsAssigned:25718224 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=149 echo kmer=145 ERR5262789 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262789-trimmed-pair1.fastq ERR5262789-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,873,017 reads, 26,071,398 reads pseudoaligned [quant] estimated average fragment length: 240.881 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,081 rounds 52973 ERR5262789.ke.tsv 35125 ERR5262789.se.tsv 88098 total ==> ERR5262789.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 696.511 0 0 PNS24247 1044 804.119 100.781 6.68872 PNS24249 1928 1688.12 444.121 14.0405 PNS24246 1044 804.119 100.781 6.68872 PNS24248 1044 804.119 100.781 6.68872 PNS24244 1471 1231.12 281.537 12.2045 PNS24243 293 110.601 1 0.482534 KQK14069 1603 1363.12 24374.3 954.297 KQK14071 474 254.664 754.265 158.068 ==> ERR5262789.se.tsv <== BRADI_1g14170v3 27157 BRADI_1g53295v3 171 BRADI_1g59795v3 508 BRADI_1g07683v3 0 BRADI_1g00485v3 27 BRADI_1g20270v3 1192 BRADI_1g74790v3 3022 BRADI_1g09890v3 0 BRADI_1g77505v3 249 BRADI_1g48960v3 0 ERR5262789 completed mapping pipeline successfully