Starting /dee2/code/volunteer_pipeline.sh ERR5262790 current disk space = 1551526068224 free memory = 1602241508 ERR5262790 SRAfilesize d6262ea45dcfacbfcd584ddd14265103 ERR5262790.sra ERR5262790.sra file validated ERR5262790 is paired end ERR5262790 is conventional basespace ERR5262790 read1 length is 70-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262790_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.582 37.0 37.0 37.0 37.0 37.0 2 36.5245 37.0 37.0 37.0 37.0 37.0 3 36.5105 37.0 37.0 37.0 37.0 37.0 4 36.7205 37.0 37.0 37.0 37.0 37.0 5 36.8005 37.0 37.0 37.0 37.0 37.0 6 36.775 37.0 37.0 37.0 37.0 37.0 7 36.663 37.0 37.0 37.0 37.0 37.0 8 36.6805 37.0 37.0 37.0 37.0 37.0 9 36.643 37.0 37.0 37.0 37.0 37.0 10-14 36.621500000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.6589 37.0 37.0 37.0 37.0 37.0 20-24 36.4582 37.0 37.0 37.0 37.0 37.0 25-29 36.2654 37.0 37.0 37.0 37.0 37.0 30-34 36.3631 37.0 37.0 37.0 37.0 37.0 35-39 36.572500000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.544 37.0 37.0 37.0 37.0 37.0 45-49 36.4793 37.0 37.0 37.0 37.0 37.0 50-54 36.4719 37.0 37.0 37.0 37.0 37.0 55-59 36.411500000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.227799999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.048500000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.174724425552874 37.0 37.0 37.0 37.0 37.0 75-79 36.39249560541664 37.0 37.0 37.0 37.0 37.0 80-84 36.49756792150124 37.0 37.0 37.0 37.0 37.0 85-89 36.44695636177407 37.0 37.0 37.0 37.0 37.0 90-94 36.435929045506015 37.0 37.0 37.0 37.0 37.0 95-99 36.50411584673329 37.0 37.0 37.0 37.0 37.0 100-104 36.40348024964521 37.0 37.0 37.0 37.0 37.0 105-109 36.52784012919356 37.0 37.0 37.0 37.0 37.0 110-114 36.430269009539984 37.0 37.0 37.0 37.0 37.0 115-119 36.47308086461932 37.0 37.0 37.0 37.0 37.0 120-124 36.350137854000955 37.0 37.0 37.0 37.0 37.0 125-129 36.326096723551856 37.0 37.0 37.0 37.0 37.0 130-134 36.456454698457605 37.0 37.0 37.0 37.0 37.0 135-139 36.37427714430307 37.0 37.0 37.0 37.0 37.0 140-144 36.37701155896284 37.0 37.0 37.0 37.0 37.0 145-149 36.32638882572629 37.0 37.0 37.0 37.0 37.0 150 36.3505853357979 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 0.0 26 0.0 27 1.0 28 5.0 29 14.0 30 8.0 31 19.0 32 28.0 33 50.0 34 91.0 35 270.0 36 2976.0 37 536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.05 20.3 3.375 34.275 2 25.650650650650654 16.716716716716718 22.42242242242242 35.21021021021021 3 9.15 17.625 38.45 34.775 4 15.425 13.25 37.8 33.525 5 34.775 19.900000000000002 27.05 18.275 6 31.85 29.349999999999998 24.075 14.725 7 17.65 14.149999999999999 53.900000000000006 14.299999999999999 8 30.65 22.525000000000002 34.725 12.1 9 16.275000000000002 13.05 41.5 29.175 10-14 24.48 25.624999999999996 30.06 19.835 15-19 26.125 20.5 31.419999999999998 21.955 20-24 28.689999999999998 19.794999999999998 28.49 23.025000000000002 25-29 21.935 22.725 36.34 19.0 30-34 20.31 24.13 36.28 19.28 35-39 24.01 20.369999999999997 31.295 24.325 40-44 25.025 19.82 35.38 19.775000000000002 45-49 18.415 17.43 33.39 30.764999999999997 50-54 16.685 14.745 41.875 26.695 55-59 22.165000000000003 12.845 31.979999999999997 33.01 60-64 15.634999999999998 23.445 28.185 32.735 65-69 18.44 22.805 35.845 22.91 70-74 29.66873498799039 20.501401120896716 31.935548438751 17.89431545236189 75-79 22.977913557369657 19.66244303100115 27.991185456002405 29.368457955626788 80-84 20.4366373902133 24.416562107904642 21.66624843161857 33.480552070263485 85-89 25.263689124400706 17.335352006056016 29.59878879636639 27.802170073176885 90-94 27.160870449461054 20.246085011185684 28.335367093756354 24.257677445596908 95-99 22.4120294599018 29.86395253682488 20.243453355155484 27.480564648117838 100-104 25.54748286700675 26.119441438656153 22.440356572370796 25.8927191219663 105-109 30.873877148346228 13.084791526039774 31.133496027831143 24.907835297782853 110-114 18.21000630649569 21.389531217153667 29.60374185410973 30.79672062224091 115-119 19.955240581872435 25.92849150103906 24.441839398944957 29.674428518143547 120-124 23.367159667494334 18.28781172406348 32.79714995141963 25.54787865702256 125-129 18.910557427766424 19.97127230539749 37.04767692392685 24.070493342909234 130-134 17.77677258848807 23.408345584077804 29.48094538052697 29.33393644690716 135-139 27.131513359995363 22.73227844432852 21.120964469947257 29.01524372572886 140-144 20.863649807749187 15.41555752735877 37.923691215616685 25.79710144927536 145-149 26.810009718172985 20.711856171039845 30.86734693877551 21.610787172011662 150 10.258780036968576 25.107825015403574 16.60505237215034 48.02834257547751 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.5 29 1.0 30 0.5 31 0.0 32 1.0 33 2.0 34 1.0 35 1.5 36 5.5 37 10.0 38 15.0 39 17.5 40 11.0 41 5.0 42 28.0 43 138.0 44 308.5 45 215.5 46 93.0 47 348.0 48 528.5 49 638.5 50 590.0 51 279.5 52 141.5 53 342.5 54 284.0 55 9.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70-71 3.0 72-73 3.0 74-75 0.0 76-77 1.0 78-79 2.0 80-81 2.0 82-83 13.0 84-85 10.0 86-87 8.0 88-89 14.0 90-91 10.0 92-93 8.0 94-95 4.0 96-97 24.0 98-99 8.0 100-101 9.0 102-103 6.0 104-105 11.0 106-107 23.0 108-109 25.0 110-111 11.0 112-113 8.0 114-115 36.0 116-117 18.0 118-119 10.0 120-121 26.0 122-123 29.0 124-125 52.0 126-127 21.0 128-129 22.0 130-131 43.0 132-133 49.0 134-135 22.0 136-137 35.0 138-139 30.0 140-141 16.0 142-143 37.0 144-145 38.0 146-147 48.0 148-149 19.0 150-151 3246.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.525 #Duplication Level Percentage of deduplicated Percentage of total 1 35.77712609970675 3.05 2 16.715542521994134 2.85 3 8.797653958944283 2.25 4 6.158357771260997 2.1 5 5.865102639296188 2.5 6 2.0527859237536656 1.05 7 3.225806451612903 1.925 8 2.0527859237536656 1.4000000000000001 9 1.466275659824047 1.125 >10 13.196480938416421 25.224999999999998 >50 2.346041055718475 16.150000000000002 >100 2.0527859237536656 26.424999999999997 >500 0.2932551319648094 13.950000000000001 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTCATCCT 558 13.950000000000001 No Hit GCCCTTTATTGATATATAACACGGTGTTGCCTTCCCCTTCCTTTCTTTTG 224 5.6000000000000005 No Hit ACACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAG 215 5.375 No Hit GACGAGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGC 161 4.025 No Hit CCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGAT 121 3.025 No Hit CTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGC 121 3.025 No Hit ATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTCA 114 2.85 No Hit CGACGAGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTG 101 2.5250000000000004 No Hit CTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCG 100 2.5 No Hit ACGACGAGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTT 95 2.375 No Hit CCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGA 91 2.275 No Hit CACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTCCCCT 88 2.1999999999999997 No Hit CCTTTATTGATATATAACACGGTGTTGCCTTCCCCTTCCTTTCTTTTGAT 87 2.175 No Hit GTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTCATCCTT 68 1.7000000000000002 No Hit CACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGC 65 1.625 No Hit GAGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTT 52 1.3 No Hit CTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCA 50 1.25 No Hit ACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTCCCC 49 1.225 No Hit CGAGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCT 47 1.175 No Hit CCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGG 44 1.0999999999999999 No Hit ACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTCCCCTT 40 1.0 No Hit GATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTC 38 0.95 No Hit GTCCTTTATTGATATATAACACGGTGTTGCCTTCCCCTTCCTTTCTTTTG 38 0.95 No Hit GTGGACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATT 31 0.775 No Hit CCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACG 30 0.75 No Hit GGACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTAC 28 0.7000000000000001 No Hit GCTTGGTAAGTGGACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCT 28 0.7000000000000001 No Hit GTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTCC 26 0.65 No Hit GTCTAATAAAAGCAAAGCTTGGTAAGTGGACAGGAGGCTGTGCTGACACG 25 0.625 No Hit CACCATCACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAA 25 0.625 No Hit CCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCC 24 0.6 No Hit AGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTC 24 0.6 No Hit CATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGGACAGGAG 23 0.575 No Hit CTTGGTAAGTGGACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTG 22 0.5499999999999999 No Hit CCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGGACAGGA 22 0.5499999999999999 No Hit ATCACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGG 21 0.525 No Hit CAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGGACAGGAGGCT 20 0.5 No Hit GCTTCCTGAAGACATTACAATTGCCCTTCCGGTCCTGACAACAAGGACAA 19 0.475 No Hit GCTGAGCTTCCTGAAGACATTACAATTGCCCTTCCGGTCCTGACAACAAG 19 0.475 No Hit TTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGA 18 0.44999999999999996 No Hit CCCTTTATTGATATATAACACGGTGTTGCCTTCCCCTTCCTTTCTTTTGA 18 0.44999999999999996 No Hit AGCTTGGTAAGTGGACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCC 18 0.44999999999999996 No Hit GGTAAGTGGACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAG 17 0.42500000000000004 No Hit CCACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAG 17 0.42500000000000004 No Hit AACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGC 17 0.42500000000000004 No Hit CTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCAC 16 0.4 No Hit GGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTCATCC 16 0.4 No Hit AACACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTA 15 0.375 No Hit ATCACCATCACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGT 14 0.35000000000000003 No Hit TTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTT 14 0.35000000000000003 No Hit GCACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAG 14 0.35000000000000003 No Hit GTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCCCTTCC 14 0.35000000000000003 No Hit GCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCCCT 14 0.35000000000000003 No Hit GTGCTGAGCTTCCTGAAGACATTACAATTGCCCTTCCGGTCCTGACAACA 14 0.35000000000000003 No Hit CACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGGAC 13 0.325 No Hit GGGCAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTAC 13 0.325 No Hit GGCCCTTCCGGTCCTGACAACAAGGACAATATGCCAAACAACAGGAATAG 13 0.325 No Hit GACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACA 11 0.27499999999999997 No Hit GCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACG 10 0.25 No Hit CTAATAAAAGCAAAGCTTGGTAAGTGGACAGGAGGCTGTGCTGACACGTT 10 0.25 No Hit CCTGAAGACATTACAATTGCCCTTCCGGTCCTGACAACAAGGACAATATG 10 0.25 No Hit CTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCCCTT 9 0.22499999999999998 No Hit AGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCC 9 0.22499999999999998 No Hit GCACATTAAAAGAAATATTGCACCAAGAGGCGGAAAGTTGAACCATGCCA 9 0.22499999999999998 No Hit GACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCCCTTCCGGTCC 9 0.22499999999999998 No Hit GACACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTA 9 0.22499999999999998 No Hit AGCCCTTCCGGTCCTGACAACAAGGACAATATGCCAAACAACAGGAATAG 8 0.2 No Hit GCCTTTTATTGATATATAACACGGTGTTGCCTTCCCCTTCCTTTCTTTTG 8 0.2 No Hit GGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTG 8 0.2 No Hit CTGAAGACATTACAATTGCCCTTCCGGTCCTGACAACAAGGACAATATGC 8 0.2 No Hit ATACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAG 8 0.2 No Hit GTCGGAGATCTCTGAGTCGGGACAACCACGTTGCAATCGAAAATCGGTCC 8 0.2 No Hit ACCATCACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAG 8 0.2 No Hit GCTCTTTATTGATATATAACACGGTGTTGCCTTCCCCTTCCTTTCTTTTG 7 0.17500000000000002 No Hit ATCGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGC 7 0.17500000000000002 No Hit ACCGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGC 7 0.17500000000000002 No Hit TTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCAC 7 0.17500000000000002 No Hit GGCGAGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGC 7 0.17500000000000002 No Hit TTTTTTTTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTG 7 0.17500000000000002 No Hit CACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGGACAG 7 0.17500000000000002 No Hit ATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGGACAGGAGG 7 0.17500000000000002 No Hit GGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCCC 7 0.17500000000000002 No Hit CCCTCCCTTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGAT 7 0.17500000000000002 No Hit TTTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCT 7 0.17500000000000002 No Hit GGTTGGTGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTCATCCT 6 0.15 No Hit GACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTCCCCT 6 0.15 No Hit AGCTTCCTGAAGACATTACAATTGCCCTTCCGGTCCTGACAACAAGGACA 6 0.15 No Hit CACACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTA 6 0.15 No Hit CGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTCATCC 6 0.15 No Hit ACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTGGACAGG 6 0.15 No Hit GCACCCTCAAGGATTTCTTATTCCTACTTTAAACAGGGATTTCTATGTCC 6 0.15 No Hit GCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCCCTTCCGG 5 0.125 No Hit AGCACATTAAAAGAAATATTGCACCAAGAGGCGGAAAGTTGAACCATGCC 5 0.125 No Hit TTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGAT 5 0.125 No Hit TGGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTCGATTTCATC 5 0.125 No Hit CTGCATTATCACGTATGTTAATACACAATTTACAGGATAAGATCGCACAA 5 0.125 No Hit TCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGGCTTC 5 0.125 No Hit CCATCACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGT 5 0.125 No Hit CATCACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTTGGTAAGTG 5 0.125 No Hit CAAGGATTTCTTATTCCTACTTTAAACAGGGATTTCTATGTCCGTGTAAA 5 0.125 No Hit CACATTAAAAGAAATATTGCACCAAGAGGCGGAAAGTTGAACCATGCCAA 5 0.125 No Hit GACGGGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGC 5 0.125 No Hit AGAAGAGGAGGAAAGAAGAATCGGATGAACTGACGATGAACCCATGCATG 5 0.125 No Hit CCATTCTTTTGATTAGGTTGATGCAGTAGCAGTGTAGGCCACGATCTTGG 5 0.125 No Hit GCCGAGTACACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGC 5 0.125 No Hit ACACCGGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTCCCC 5 0.125 No Hit ACAGGAGGCTGTGCTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAA 5 0.125 No Hit TTCATCACCATCACACCATCAATAAGATAAGTCTAATAAAAGCAAAGCTT 5 0.125 No Hit GCACCAGGTGCTGGCCCTTTATTGATATATAACACGGTGTTGCCTTCCCC 5 0.125 No Hit AGCACGGTGTTGCCTTCCCCTTCCTTTCTTTTGATTAGGTTGATGCAGTA 5 0.125 No Hit CTGACACGTTGTGCTGAGCTTCCTGAAGACATTACAATTGCCCTTCCGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGAGTA 30 0.0021825281 69.0375 2 GACGAGT 30 0.0021825281 69.0375 1 CGAGTAC 45 1.3441099E-4 61.36666 3 AGTACAC 50 2.2634289E-4 55.230003 5 GAGTACA 50 2.2634289E-4 55.230003 4 GTACACC 55 3.6247267E-4 50.209087 6 TACACCA 55 3.6247267E-4 50.209087 7 ACACCAG 60 5.5688195E-4 46.024998 8 CACCAGG 70 0.0011901121 39.45 9 CCTTGAG 40 5.6757926E-8 34.955696 140-144 TGAGCTC 35 8.0809514E-7 34.955696 140-144 TTGAGCT 45 1.5979276E-7 31.071732 140-144 CTTGAGC 45 1.5979276E-7 31.071732 140-144 CGACTTC 60 1.0186341E-10 30.406906 135-139 TTTATTG 115 3.5161807E-4 30.016306 5 GCCCTTT 95 0.005326558 29.068419 1 CTTTATT 120 4.5131566E-4 28.765623 4 TTATTGA 120 4.5131566E-4 28.765623 6 TATTGAT 120 4.5131566E-4 28.765623 7 CCTTTAT 120 4.5131566E-4 28.765623 3 >>END_MODULE ERR5262790 read2 length is 70-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262790_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-150 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.475 37.0 37.0 37.0 37.0 37.0 2 36.363 37.0 37.0 37.0 37.0 37.0 3 36.391 37.0 37.0 37.0 37.0 37.0 4 36.403 37.0 37.0 37.0 37.0 37.0 5 36.436 37.0 37.0 37.0 37.0 37.0 6 36.4195 37.0 37.0 37.0 37.0 37.0 7 36.4495 37.0 37.0 37.0 37.0 37.0 8 36.505 37.0 37.0 37.0 37.0 37.0 9 36.4025 37.0 37.0 37.0 37.0 37.0 10-14 36.4448 37.0 37.0 37.0 37.0 37.0 15-19 36.5103 37.0 37.0 37.0 37.0 37.0 20-24 36.467699999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.4735 37.0 37.0 37.0 37.0 37.0 30-34 36.433299999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.4469 37.0 37.0 37.0 37.0 37.0 40-44 36.4442 37.0 37.0 37.0 37.0 37.0 45-49 36.3622 37.0 37.0 37.0 37.0 37.0 50-54 36.2808 37.0 37.0 37.0 37.0 37.0 55-59 36.3366 37.0 37.0 37.0 37.0 37.0 60-64 36.3096 37.0 37.0 37.0 37.0 37.0 65-69 36.301199999999994 37.0 37.0 37.0 37.0 37.0 70-74 36.246681301013965 37.0 37.0 37.0 37.0 37.0 75-79 36.272498329261985 37.0 37.0 37.0 37.0 37.0 80-84 36.25166743878665 37.0 37.0 37.0 37.0 37.0 85-89 36.343172657962704 37.0 37.0 37.0 37.0 37.0 90-94 36.227383466692096 37.0 37.0 37.0 37.0 37.0 95-99 36.18755637027174 37.0 37.0 37.0 37.0 37.0 100-104 36.154073052514846 37.0 37.0 37.0 37.0 37.0 105-109 36.13974220565849 37.0 37.0 37.0 37.0 37.0 110-114 36.240402947041325 37.0 37.0 37.0 37.0 37.0 115-119 36.181542951726456 37.0 37.0 37.0 37.0 37.0 120-124 36.1325861416082 37.0 37.0 37.0 37.0 37.0 125-129 36.11277747362841 37.0 37.0 37.0 37.0 37.0 130-134 36.04817299107595 37.0 37.0 37.0 37.0 37.0 135-139 36.05012839001284 37.0 37.0 37.0 37.0 37.0 140-144 35.99876611849301 37.0 37.0 37.0 37.0 37.0 145-149 35.968121687273886 37.0 37.0 37.0 37.0 37.0 150 35.99475794017885 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 3.0 23 2.0 24 3.0 25 4.0 26 6.0 27 5.0 28 5.0 29 6.0 30 10.0 31 20.0 32 34.0 33 51.0 34 105.0 35 430.0 36 2925.0 37 391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.5 25.35 6.125 31.025000000000002 2 27.500000000000004 30.45 22.175 19.875 3 16.35 28.925 28.325 26.400000000000002 4 21.95 37.425000000000004 14.475 26.150000000000002 5 21.45 47.125 14.374999999999998 17.05 6 18.875 45.9 13.3 21.925 7 27.875 17.224999999999998 31.574999999999996 23.325000000000003 8 18.45 27.825 18.025 35.699999999999996 9 22.35 27.150000000000002 19.3 31.2 10-14 21.12 33.31 18.16 27.41 15-19 22.855 28.92 21.310000000000002 26.915 20-24 22.220000000000002 31.59 22.465 23.724999999999998 25-29 23.119999999999997 26.095000000000002 22.73 28.055000000000003 30-34 22.805 25.335 25.275 26.584999999999997 35-39 22.96 25.615 27.084999999999997 24.34 40-44 25.180000000000003 28.15 22.355 24.315 45-49 26.305 26.935 20.16 26.6 50-54 29.86 27.334999999999997 19.155 23.65 55-59 33.739999999999995 24.05 19.915 22.295 60-64 29.79 25.290000000000003 19.37 25.55 65-69 32.635 25.03 18.895 23.44 70-74 30.499399519615693 27.356885508406727 18.379703763010408 23.764011208967172 75-79 28.642259728552112 26.8893674563029 21.08478990334051 23.383582911804478 80-84 25.696361355081553 27.51819322459222 21.304893350062734 25.48055207026349 85-89 24.950794852384558 28.942720161493817 18.349735049205147 27.756749936916474 90-94 21.812080536912752 33.70449461053488 18.64958307911328 25.833841773439087 95-99 24.900255754475705 32.21994884910486 18.276214833759592 24.603580562659847 100-104 23.85590599876314 31.679035250463823 20.05256648113791 24.412492269635127 105-109 26.28381535905291 29.98078820291812 18.75486785399034 24.98052858403863 110-114 22.33550557073786 30.02417490014715 19.04561698549506 28.594702543619928 115-119 26.24287312836362 26.274844141311878 20.00852560345287 27.473757126871632 120-124 27.849195203629684 28.74581397861078 18.337474343739874 25.067516474019662 125-129 26.622084668951036 28.871449099148887 19.592129987841272 24.914336244058806 130-134 27.791914051456036 28.346055979643765 20.60503251342946 23.25699745547074 135-139 26.435982148032227 28.047296122413496 21.972990204602098 23.543731524952182 140-144 27.737355811889973 26.459627329192546 22.744750073942622 23.058266784974858 145-149 26.537806953561876 26.07585703865791 24.805494772672017 22.580841235108192 150 28.831329016342895 28.862164662349677 21.893308664816526 20.413197656490905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 0.5 28 1.0 29 1.0 30 2.0 31 3.0 32 4.0 33 7.0 34 8.0 35 4.5 36 10.5 37 15.5 38 14.0 39 19.5 40 18.0 41 26.0 42 42.0 43 46.5 44 52.5 45 74.5 46 75.0 47 79.0 48 127.0 49 205.5 50 343.5 51 487.0 52 670.0 53 529.5 54 311.5 55 303.5 56 200.0 57 123.5 58 88.0 59 66.0 60 27.0 61 3.0 62 2.0 63 1.5 64 1.0 65 1.0 66 1.0 67 1.5 68 3.0 69 2.5 70 1.5 71 1.0 72 1.5 73 4.5 74 3.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70-71 3.0 72-73 3.0 74-75 0.0 76-77 1.0 78-79 2.0 80-81 2.0 82-83 13.0 84-85 10.0 86-87 8.0 88-89 14.0 90-91 10.0 92-93 8.0 94-95 4.0 96-97 25.0 98-99 8.0 100-101 9.0 102-103 5.0 104-105 11.0 106-107 23.0 108-109 25.0 110-111 11.0 112-113 8.0 114-115 36.0 116-117 18.0 118-119 10.0 120-121 29.0 122-123 29.0 124-125 50.0 126-127 21.0 128-129 22.0 130-131 42.0 132-133 49.0 134-135 22.0 136-137 35.0 138-139 30.0 140-141 16.0 142-143 37.0 144-145 41.0 146-147 48.0 148-149 19.0 150-151 3243.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.9 #Duplication Level Percentage of deduplicated Percentage of total 1 35.84905660377358 5.7 2 15.40880503144654 4.9 3 8.647798742138365 4.125 4 6.446540880503145 4.1000000000000005 5 4.088050314465408 3.25 6 6.446540880503145 6.15 7 3.1446540880503147 3.5000000000000004 8 1.729559748427673 2.1999999999999997 9 2.044025157232704 2.9250000000000003 >10 14.622641509433961 43.05 >50 1.4150943396226416 15.075 >100 0.15723270440251574 5.025 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGA 201 5.025 No Hit AACAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACC 86 2.15 No Hit GTTGACGAGCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCC 84 2.1 No Hit CCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGG 81 2.025 No Hit CCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGC 69 1.725 No Hit GTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCG 67 1.675 No Hit ACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAG 58 1.4500000000000002 No Hit GAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGG 55 1.375 No Hit CTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGG 52 1.3 No Hit AGCTAACAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACT 51 1.275 No Hit CAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGA 48 1.2 No Hit CTAACAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCA 47 1.175 No Hit CGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCCA 44 1.0999999999999999 No Hit AGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAGGAG 41 1.0250000000000001 No Hit CAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTA 39 0.975 No Hit AGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCCAAG 38 0.95 No Hit AGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCG 35 0.8750000000000001 No Hit CATAGCTCAAGCACTCTGCACTACTGCTGCTGTCATGGGAGGGAAGAGCC 33 0.8250000000000001 No Hit GCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTG 33 0.8250000000000001 No Hit AGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACT 31 0.775 No Hit AGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAG 31 0.775 No Hit TGATCATTGACTTCACTGCTGTCTGGTGCGGACCTTGCCGCATCATAGCC 30 0.75 No Hit GTTGAATTGTCAAGTGGAGTGCTATTCCTGTTGTTTGGCATATTGTCCTT 29 0.7250000000000001 No Hit ATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAG 28 0.7000000000000001 No Hit CCTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTTCCC 28 0.7000000000000001 No Hit ATTGACTTCACTGCTGTCTGGTGCGGACCTTGCCGCATCATAGCCCCTGT 28 0.7000000000000001 No Hit GACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGA 27 0.675 No Hit AGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTC 26 0.65 No Hit GTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGCTCAAGGAAG 25 0.625 No Hit CAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGCTCA 24 0.6 No Hit GTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAA 24 0.6 No Hit AGGAGATCGGCAAGCTGGTGATCATTGACTTCACTGCTGTCTGGTGCGGA 23 0.575 No Hit CAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGAC 23 0.575 No Hit CAACAAGCAAGAATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCA 23 0.575 No Hit GGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCA 22 0.5499999999999999 No Hit GCCAAGGCCAAGGAGATCGGCAAGCTGGTGATCATTGACTTCACTGCTGT 21 0.525 No Hit GCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGT 21 0.525 No Hit GCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGA 20 0.5 No Hit AGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATC 19 0.475 No Hit AGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCC 18 0.44999999999999996 No Hit GGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCCAAGATCG 18 0.44999999999999996 No Hit GGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGG 18 0.44999999999999996 No Hit GCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCC 18 0.44999999999999996 No Hit GCTATTCCTGTTGTTTGGCATATTGTCCTTGTTGTCAGGACCGGAAGGGC 17 0.42500000000000004 No Hit CGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGG 17 0.42500000000000004 No Hit CTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTTCCCT 17 0.42500000000000004 No Hit GCTAACAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTC 17 0.42500000000000004 No Hit GGAAGAGCCTTGCCTCGCAGATATCTGAGAAGATGGTTGAATTGTCAAGT 17 0.42500000000000004 No Hit GAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCCAA 17 0.42500000000000004 No Hit AATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCC 16 0.4 No Hit GCCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGCT 16 0.4 No Hit GGCAAGAAGGATGAAATCGAAGCCAAGATCGTGGCCTACACTGCTACTGC 16 0.4 No Hit TCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGG 16 0.4 No Hit GAATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCAAGCTGGTGAT 15 0.375 No Hit CAGATATCTGAGAAGATGGTTGAATTGTCAAGTGGAGTGCTATTCCTGTT 15 0.375 No Hit GGCCAAGGCCAAGGAGATCGGCAAGCTGGTGATCATTGACTTCACTGCTG 15 0.375 No Hit AGGGAACCGTGATCGCGTGCCACAACAAGCAAGAATTCGACACCCACATG 15 0.375 No Hit CCTCAAGGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGAATACAAAA 15 0.375 No Hit TAACAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCAC 15 0.375 No Hit GAGGGAACCGTGATCGCGTGCCACAACAAGCAAGAATTCGACACCCACAT 14 0.35000000000000003 No Hit GGAGGGAACCGTGATCGCGTGCCACAACAAGCAAGAATTCGACACCCACA 14 0.35000000000000003 No Hit CAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCT 14 0.35000000000000003 No Hit CGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGT 14 0.35000000000000003 No Hit CGAAAACCCATTTGGTGTTGTCATTGGGGGAGTCATAGCTCAAGCACTCT 14 0.35000000000000003 No Hit GTCTTCGCTGAGTACGCCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGT 14 0.35000000000000003 No Hit CTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCT 14 0.35000000000000003 No Hit CAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGG 14 0.35000000000000003 No Hit CGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAG 14 0.35000000000000003 No Hit ATCTTCCTCAAGGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGAATA 14 0.35000000000000003 No Hit CATTGACTTCACTGCTGTCTGGTGCGGACCTTGCCGCATCATAGCCCCTG 13 0.325 No Hit GCCAAGGAGATCGGCAAGCTGGTGATCATTGACTTCACTGCTGTCTGGTG 13 0.325 No Hit CGGCCGAGGAGGGAACCGTGATCGCGTGCCACAACAAGCAAGAATTCGAC 13 0.325 No Hit AGATCGGCAAGCTGGTGATCATTGACTTCACTGCTGTCTGGTGCGGACCT 13 0.325 No Hit GGAACCGTGATCGCGTGCCACAACAAGCAAGAATTCGACACCCACATGGC 13 0.325 No Hit AAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAGGAGGG 13 0.325 No Hit GGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTG 13 0.325 No Hit AAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTT 13 0.325 No Hit GGAATGGCGGCCGAGGAGGGAACCGTGATCGCGTGCCACAACAAGCAAGA 13 0.325 No Hit TGTTGACGAGCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGC 13 0.325 No Hit GCGGACCTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAG 13 0.325 No Hit CTGGTGCGGACCTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCA 13 0.325 No Hit AATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAG 12 0.3 No Hit GTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGC 12 0.3 No Hit GTCATGGGAGGGAAGAGCCTTGCCTCGCAGATATCTGAGAAGATGGTTGA 12 0.3 No Hit CCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCCAAGATCGTGG 12 0.3 No Hit CTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCA 12 0.3 No Hit CATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTTCCCTGGTGCCATCT 12 0.3 No Hit AAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAGGA 11 0.27499999999999997 No Hit AAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGC 11 0.27499999999999997 No Hit CTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAGGAGGGAACCGTGAT 11 0.27499999999999997 No Hit CTTACTCACCTAGAGGAATGGCGGCCGAGGAGGGAACCGTGATCGCGTGC 11 0.27499999999999997 No Hit GCCACAACAAGCAAGAATTCGACACCCACATGGCCAAGGCCAAGGAGATC 11 0.27499999999999997 No Hit GTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCCAAGATCGT 10 0.25 No Hit CGGCAAGCTGGTGATCATTGACTTCACTGCTGTCTGGTGCGGACCTTGCC 10 0.25 No Hit GTGCGGACCTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGA 10 0.25 No Hit AATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCG 10 0.25 No Hit AGTGGGGTGACAAGAGCCAGATTGCTACAATTGGCTTGGCTGCTGACGAA 10 0.25 No Hit GCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCC 10 0.25 No Hit GGGAGGGAAGAGCCTTGCCTCGCAGATATCTGAGAAGATGGTTGAATTGT 10 0.25 No Hit TCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAG 10 0.25 No Hit GATTGCTACAATTGGCTTGGCTGCTGACGAAAACCCATTTGGTGTTGTCA 10 0.25 No Hit CTTCCTCAAGGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGAATACA 10 0.25 No Hit AAGCAAGAATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCAAGCT 10 0.25 No Hit CCTGTCTTCGCTGAGTACGCCAAGAAGTTCCCTGGTGCCATCTTCCTCAA 9 0.22499999999999998 No Hit ATGTTGACGAGCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATG 9 0.22499999999999998 No Hit CCCATTTGGTGTTGTCATTGGGGGAGTCATAGCTCAAGCACTCTGCACTA 9 0.22499999999999998 No Hit CTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAA 9 0.22499999999999998 No Hit CAAGAAGGATGAAATCGAAGCCAAGATCGTGGCCTACACTGCTACTGCAT 9 0.22499999999999998 No Hit GATCGGCAAGCTGGTGATCATTGACTTCACTGCTGTCTGGTGCGGACCTT 9 0.22499999999999998 No Hit CTAGCTAACAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTA 9 0.22499999999999998 No Hit CAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAA 9 0.22499999999999998 No Hit ATCTTACTCACCTAGAGGAATGGCGGCCGAGGAGGGAACCGTGATCGCGT 9 0.22499999999999998 No Hit AATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAA 9 0.22499999999999998 No Hit AGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACG 9 0.22499999999999998 No Hit GACCATTTCTCATGCAGTTCTTCTCACCAATTTTTCTAAAGGCATTTTCC 9 0.22499999999999998 No Hit CGCTGAGTACGCCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATG 9 0.22499999999999998 No Hit GAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGCTCAAGG 8 0.2 No Hit GAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAGGAGG 8 0.2 No Hit GTCAAGTGGAGTGCTATTCCTGTTGTTTGGCATATTGTCCTTGTTGTCAG 8 0.2 No Hit AGCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTC 8 0.2 No Hit CAAAGCTGGATGCGGACTTCAAGAGTAACAAAGGGGAACAGAAAAACAAA 8 0.2 No Hit CTTCAAGAGTAACAAAGGGGAACAGAAAAACAAATCAAAGGCAACTGATG 8 0.2 No Hit GGTGACAAGAGCCAGATTGCTACAATTGGCTTGGCTGCTGACGAAAACCC 8 0.2 No Hit CAAGAATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCAAGCTGGT 8 0.2 No Hit AGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGCTCAAG 8 0.2 No Hit CTCACCTAGAGGAATGGCGGCCGAGGAGGGAACCGTGATCGCGTGCCACA 8 0.2 No Hit CCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGA 8 0.2 No Hit CATCTTCCTCAAGGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGAAT 7 0.17500000000000002 No Hit GCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTTCCCTGGT 7 0.17500000000000002 No Hit GGGGAACAGAAAAACAAATCAAAGGCAACTGATGATACGAAGAAACAGCG 7 0.17500000000000002 No Hit GGCCGAGGAGGGAACCGTGATCGCGTGCCACAACAAGCAAGAATTCGACA 7 0.17500000000000002 No Hit GACCTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTTC 7 0.17500000000000002 No Hit TACAAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAA 7 0.17500000000000002 No Hit GAGATCGGCAAGCTGGTGATCATTGACTTCACTGCTGTCTGGTGCGGACC 7 0.17500000000000002 No Hit CGAGTGGGGTGACAAGAGCCAGATTGCTACAATTGGCTTGGCTGCTGACG 7 0.17500000000000002 No Hit GACGAGCTCAAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGAC 7 0.17500000000000002 No Hit CAAGAGCCAGATTGCTACAATTGGCTTGGCTGCTGACGAAAACCCATTTG 7 0.17500000000000002 No Hit AGTCATAGCTCAAGCACTCTGCACTACTGCTGCTGTCATGGGAGGGAAGA 7 0.17500000000000002 No Hit GGAGGGAAGAGCCTTGCCTCGCAGATATCTGAGAAGATGGTTGAATTGTC 7 0.17500000000000002 No Hit GGGAGTCATAGCTCAAGCACTCTGCACTACTGCTGCTGTCATGGGAGGGA 7 0.17500000000000002 No Hit GGGAAGAGCCTTGCCTCGCAGATATCTGAGAAGATGGTTGAATTGTCAAG 7 0.17500000000000002 No Hit GCCAGATTGCTACAATTGGCTTGGCTGCTGACGAAAACCCATTTGGTGTT 7 0.17500000000000002 No Hit ATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGA 7 0.17500000000000002 No Hit CCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGC 7 0.17500000000000002 No Hit GTTGTCATTGGGGGAGTCATAGCTCAAGCACTCTGCACTACTGCTGCTGT 7 0.17500000000000002 No Hit GCCTTGCCTCGCAGATATCTGAGAAGATGGTTGAATTGTCAAGTGGAGTG 7 0.17500000000000002 No Hit CATATTGTCCTTGTTGTCAGGACCGGAAGGGCAATTGTAATGTCTTCAGG 7 0.17500000000000002 No Hit AAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCGAAGCCAAGAT 6 0.15 No Hit AACCGTGATCGCGTGCCACAACAAGCAAGAATTCGACACCCACATGGCCA 6 0.15 No Hit AACAAGCAAGAATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCAA 6 0.15 No Hit AGCAAGAATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCAAGCTG 6 0.15 No Hit GCTGACGAAAACCCATTTGGTGTTGTCATTGGGGGAGTCATAGCTCAAGC 6 0.15 No Hit CTTCATACCTCTCCGGTCTGTATGATGCTTTTTCTTCTTCTTCTTCTTTT 6 0.15 No Hit GTACGCCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACG 6 0.15 No Hit GAGGGAAGAGCCTTGCCTCGCAGATATCTGAGAAGATGGTTGAATTGTCA 6 0.15 No Hit CGGACCTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGT 6 0.15 No Hit AGAGGAATGGCGGCCGAGGAGGGAACCGTGATCGCGTGCCACAACAAGCA 6 0.15 No Hit GGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAAATCG 6 0.15 No Hit GGCCAAGGAGATCGGCAAGCTGGTGATCATTGACTTCACTGCTGTCTGGT 6 0.15 No Hit CAAAGGGGAACAGAAAAACAAATCAAAGGCAACTGATGATACGAAGAAAC 6 0.15 No Hit TTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAA 6 0.15 No Hit CATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTTCCCTGGTGCCA 6 0.15 No Hit ATAGCTCAAGCACTCTGCACTACTGCTGCTGTCATGGGAGGGAAGAGCCT 6 0.15 No Hit GGCAACTGATGATACGAAGAAACAGCGAAGACCATTTCTCATGCAGTTCT 6 0.15 No Hit GATCATTGACTTCACTGCTGTCTGGTGCGGACCTTGCCGCATCATAGCCC 6 0.15 No Hit TGGCGGCCGAGGAGGGAACCGTGATCGCGTGCCACAACAAGCAAGAATTC 6 0.15 No Hit ATATTGTCCTTGTTGTCAGGACCGGAAGGGCAATTGTAATGTCTTCAGGA 6 0.15 No Hit CAGGAAGTCGCAGAAGAATACAAAATCGAGGCAATGCCGACCTTCCTCTT 6 0.15 No Hit TGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTTCCCTGG 6 0.15 No Hit CTTCGCTGAGTACGCCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAG 6 0.15 No Hit GCAAGAAGGATGAAATCGAAGCCAAGATCGTGGCCTACACTGCTACTGCA 6 0.15 No Hit GCGGCCGAGGAGGGAACCGTGATCGCGTGCCACAACAAGCAAGAATTCGA 6 0.15 No Hit GCCATCTTCCTCAAGGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGA 6 0.15 No Hit AGAAGACGGAGAGTCAGAAGAATTGGCTGAAATGGAAGCAAAGCTGGATG 6 0.15 No Hit GCTGAGTACGCCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGT 6 0.15 No Hit ATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCAAGCTGGTGATCA 6 0.15 No Hit CCGTGATCGCGTGCCACAACAAGCAAGAATTCGACACCCACATGGCCAAG 6 0.15 No Hit GTGCCATCTTCCTCAAGGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAA 6 0.15 No Hit AGAAAAACAAATCAAAGGCAACTGATGATACGAAGAAACAGCGAAGACCA 6 0.15 No Hit ACTCACCTAGAGGAATGGCGGCCGAGGAGGGAACCGTGATCGCGTGCCAC 6 0.15 No Hit CCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAGGAGGGAACC 6 0.15 No Hit AAAATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGT 6 0.15 No Hit TCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAG 6 0.15 No Hit AACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAGGAGGGAA 6 0.15 No Hit CGCCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGC 6 0.15 No Hit CCCACATGGCCAAGGCCAAGGAGATCGGCAAGCTGGTGATCATTGACTTC 6 0.15 No Hit CGTGATCGCGTGCCACAACAAGCAAGAATTCGACACCCACATGGCCAAGG 6 0.15 No Hit CCAAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGCTC 6 0.15 No Hit GACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTG 5 0.125 No Hit CAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAATGGCGGCCGAG 5 0.125 No Hit GGACCTTGCCGCATCATAGCCCCTGTCTTCGCTGAGTACGCCAAGAAGTT 5 0.125 No Hit ATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTACTCACCTAGAGGAAT 5 0.125 No Hit CAATTGGCTTGGCTGCTGACGAAAACCCATTTGGTGTTGTCATTGGGGGA 5 0.125 No Hit GTTCGAGTCGCTCCACGAGTGCCTCCCGGCCGACAGCGCCGAGCGGCTCG 5 0.125 No Hit AGTGATGACAGCTTTATCTGCTTCTCTAGGCTGGGTTGCACCAAATCTGA 5 0.125 No Hit CAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCACACTGTC 5 0.125 No Hit GAGCCAGATTGCTACAATTGGCTTGGCTGCTGACGAAAACCCATTTGGTG 5 0.125 No Hit GGGTGACAAGAGCCAGATTGCTACAATTGGCTTGGCTGCTGACGAAAACC 5 0.125 No Hit TGGTGATCATTGACTTCACTGCTGTCTGGTGCGGACCTTGCCGCATCATA 5 0.125 No Hit AAGAAGTTCCCTGGTGCCATCTTCCTCAAGGTAGATGTTGACGAGCTCAA 5 0.125 No Hit CTTCACTGCTGTCTGGTGCGGACCTTGCCGCATCATAGCCCCTGTCTTCG 5 0.125 No Hit GCAAGAATTCGACACCCACATGGCCAAGGCCAAGGAGATCGGCAAGCTGG 5 0.125 No Hit CGAAGAAACAGCGAAGACCATTTCTCATGCAGTTCTTCTCACCAATTTTT 5 0.125 No Hit TCAAGGACGGCGAGAAGGTCCACACTGTCGTTGGTGGCAAGAAGGATGAA 5 0.125 No Hit TACTCACCTAGAGGAATGGCGGCCGAGGAGGGAACCGTGATCGCGTGCCA 5 0.125 No Hit GGGGTGACAAGAGCCAGATTGCTACAATTGGCTTGGCTGCTGACGAAAAC 5 0.125 No Hit CCATTGAACACTAAAATTGTTTGAGATTTTGCAAGAAAAAGTACTTCATA 5 0.125 No Hit ATCGAGGCAATGCCGACCTTCCTCTTCATCAAGGACGGCGAGAAGGTCCA 5 0.125 No Hit CTCAAGGTAGATGTTGACGAGCTCAAGGAAGTCGCAGAAGAATACAAAAT 5 0.125 No Hit GTTCTTCTCACCAATTTTTCTAAAGGCATTTTCCATTACTTTCTTTGGCG 5 0.125 No Hit AGAGCCTTGCCTCGCAGATATCTGAGAAGATGGTTGAATTGTCAAGTGGA 5 0.125 No Hit TGTGACCACTCTGCTGTTTTTTGTGTTTGGCATCTGGTCATTGTGGGAAG 5 0.125 No Hit CACATGGCCAAGGCCAAGGAGATCGGCAAGCTGGTGATCATTGACTTCAC 5 0.125 No Hit TAGCTAACAGATCAATCCCCAAGTCAAAGAAAACCTCTCTCTGATCTTAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635534 spots for ERR5262790.sra Written 1635534 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra Read 1635528 spots for ERR5262790.sra Written 1635528 spots for ERR5262790.sra SRR ids: ['ERR5262790.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_s_3bw196 ERR5262790.sra spots: 32710566 blocks: [[1, 1635528], [1635529, 3271056], [3271057, 4906584], [4906585, 6542112], [6542113, 8177640], [8177641, 9813168], [9813169, 11448696], [11448697, 13084224], [13084225, 14719752], [14719753, 16355280], [16355281, 17990808], [17990809, 19626336], [19626337, 21261864], [21261865, 22897392], [22897393, 24532920], [24532921, 26168448], [26168449, 27803976], [27803977, 29439504], [29439505, 31075032], [31075033, 32710566]] ERR5262790 file size 10530992 ERR5262790 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262790 ERR5262790_1.fastq ERR5262790_2.fastq Input file: ERR5262790_1.fastq Paired file: ERR5262790_2.fastq trimmed: ERR5262790-trimmed-pair1.fastq, ERR5262790-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:40:08 2024 >> started Fri Dec 6 11:40:44 2024 >> done (35.497s) 32710566 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 32710566 (100.00%) read pairs available; of these: 9870 ( 0.03%) trimmed read pairs available after processing 32700696 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 1 0.00% 27 3 0.00% 28 3 0.00% 29 1 0.00% 30 5 0.00% 31 1 0.00% 32 5 0.00% 33 3 0.00% 34 4 0.00% 35 1 0.00% 36 1 0.00% 37 3 0.00% 38 11 0.00% 39 0 0.00% 40 2 0.00% 41 0 0.00% 42 2 0.00% 43 0 0.00% 44 2 0.00% 45 0 0.00% 46 2 0.00% 47 3 0.00% 48 3 0.00% 49 1038 0.00% 50 1051 0.00% 51 1206 0.00% 52 1382 0.00% 53 1483 0.00% 54 1465 0.00% 55 1606 0.00% 56 1805 0.01% 57 1983 0.01% 58 2398 0.01% 59 2631 0.01% 60 3096 0.01% 61 3585 0.01% 62 3848 0.01% 63 4314 0.01% 64 4688 0.01% 65 4927 0.02% 66 5644 0.02% 67 6221 0.02% 68 6905 0.02% 69 7922 0.02% 70 9251 0.03% 71 10371 0.03% 72 11407 0.03% 73 13321 0.04% 74 14255 0.04% 75 15700 0.05% 76 16900 0.05% 77 18862 0.06% 78 20686 0.06% 79 22636 0.07% 80 24762 0.08% 81 27846 0.09% 82 31710 0.10% 83 34209 0.10% 84 37200 0.11% 85 40284 0.12% 86 43197 0.13% 87 45252 0.14% 88 47901 0.15% 89 51070 0.16% 90 54108 0.17% 91 58207 0.18% 92 61725 0.19% 93 66636 0.20% 94 71253 0.22% 95 74726 0.23% 96 76678 0.23% 97 80029 0.24% 98 81272 0.25% 99 84921 0.26% 100 87556 0.27% 101 89534 0.27% 102 93585 0.29% 103 98084 0.30% 104 100817 0.31% 105 105101 0.32% 106 106853 0.33% 107 109533 0.33% 108 110006 0.34% 109 112902 0.35% 110 113761 0.35% 111 116810 0.36% 112 119173 0.36% 113 121594 0.37% 114 125928 0.38% 115 128774 0.39% 116 129864 0.40% 117 131382 0.40% 118 131679 0.40% 119 131030 0.40% 120 132789 0.41% 121 135095 0.41% 122 135619 0.41% 123 138094 0.42% 124 141769 0.43% 125 142818 0.44% 126 142690 0.44% 127 144440 0.44% 128 143453 0.44% 129 145631 0.45% 130 146928 0.45% 131 145320 0.44% 132 146813 0.45% 133 148506 0.45% 134 149686 0.46% 135 150427 0.46% 136 151857 0.46% 137 152136 0.47% 138 152448 0.47% 139 152313 0.47% 140 150291 0.46% 141 151769 0.46% 142 152981 0.47% 143 153610 0.47% 144 155223 0.47% 145 154073 0.47% 146 158427 0.48% 147 268904 0.82% 148 152002 0.46% 149 150595 0.46% 150 24584262 75.16% 32710566 reads passed initial QC criterion=sequence-density sequence-density=3.48 sequence-density-rank=1 fanout-score=2.45 fanout-score-rank=24 prefix-density=3.94 prefix-fanout=2.2 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=57.04 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=2.0 sequence=ATTCCACATGTGCACCGATTTTTATTTAACTGGGCAGAGACGCATACATTATTATTTATTAAGACATATGTTTATTCACTGAGGTTACGACATGCAACACCGACCAGCTGATCGGCCATCGGCAGGCAACAACAGTTTTTTCTCTGGCAGAAAAGGACAGCAGTAACAAAGGCGACGATGATGGCAATTTATATATT criterion=sequence-density sequence-density=10.46 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=36 prefix-density=10.39 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.30 sequence-density-rank=15 fanout-score=75.79 fanout-score-rank=1 prefix-density=10.72 prefix-fanout=2.1 sequence=GTTCCGGTTCGCGGCTAGCAGTAGTTGTTGTAGTAGCAGCTAGGGTTTCCGGTAGGGTTCCGTCGAGATCGCCATGGATGAGTACCGCTGCTTCGTGGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262790 ERR5262790_1.fastq ERR5262790_2.fastq Input file: ERR5262790_1.fastq Paired file: ERR5262790_2.fastq trimmed: ERR5262790-trimmed-pair1.fastq, ERR5262790-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:43:20 2024 >> started Fri Dec 6 11:43:43 2024 >> done (23.557s) 24532925 read pairs processed; of these: 110 ( 0.00%) short read pairs filtered out after trimming by size control 208 ( 0.00%) empty read pairs filtered out after trimming by size control 24532607 (100.00%) read pairs available; of these: 197 ( 0.00%) trimmed read pairs available after processing 24532410 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 1 0.00% 27 3 0.00% 28 2 0.00% 29 1 0.00% 30 2 0.00% 31 1 0.00% 32 5 0.00% 33 2 0.00% 34 2 0.00% 35 1 0.00% 36 1 0.00% 37 2 0.00% 38 9 0.00% 39 0 0.00% 40 2 0.00% 41 0 0.00% 42 2 0.00% 43 0 0.00% 44 2 0.00% 45 0 0.00% 46 1 0.00% 47 3 0.00% 48 3 0.00% 49 939 0.00% 50 941 0.00% 51 1016 0.00% 52 1098 0.00% 53 1228 0.01% 54 1146 0.00% 55 666 0.00% 56 963 0.00% 57 1741 0.01% 58 2133 0.01% 59 2218 0.01% 60 1651 0.01% 61 3029 0.01% 62 3539 0.01% 63 3478 0.01% 64 3350 0.01% 65 4173 0.02% 66 3488 0.01% 67 5524 0.02% 68 4920 0.02% 69 5673 0.02% 70 7008 0.03% 71 7873 0.03% 72 8164 0.03% 73 10637 0.04% 74 10297 0.04% 75 11151 0.05% 76 13246 0.05% 77 14098 0.06% 78 15851 0.06% 79 16310 0.07% 80 18638 0.08% 81 21359 0.09% 82 24133 0.10% 83 23573 0.10% 84 27884 0.11% 85 30584 0.12% 86 33257 0.14% 87 34185 0.14% 88 34841 0.14% 89 37910 0.15% 90 40728 0.17% 91 44621 0.18% 92 45449 0.19% 93 49881 0.20% 94 54465 0.22% 95 53248 0.22% 96 57216 0.23% 97 60110 0.25% 98 59139 0.24% 99 64501 0.26% 100 66212 0.27% 101 69041 0.28% 102 70931 0.29% 103 71854 0.29% 104 78023 0.32% 105 78666 0.32% 106 80999 0.33% 107 82492 0.34% 108 82651 0.34% 109 84344 0.34% 110 83783 0.34% 111 87489 0.36% 112 88250 0.36% 113 91056 0.37% 114 92986 0.38% 115 96928 0.40% 116 97000 0.40% 117 99064 0.40% 118 98628 0.40% 119 99227 0.40% 120 98834 0.40% 121 102105 0.42% 122 101357 0.41% 123 103491 0.42% 124 106413 0.43% 125 106906 0.44% 126 106692 0.43% 127 108045 0.44% 128 107644 0.44% 129 109163 0.44% 130 110325 0.45% 131 109538 0.45% 132 110109 0.45% 133 112066 0.46% 134 112164 0.46% 135 113082 0.46% 136 114033 0.46% 137 113738 0.46% 138 114772 0.47% 139 113681 0.46% 140 112956 0.46% 141 113688 0.46% 142 114421 0.47% 143 114748 0.47% 144 116766 0.48% 145 115699 0.47% 146 119209 0.49% 147 200882 0.82% 148 114353 0.47% 149 112946 0.46% 150 18439839 75.16% criterion=sequence-density sequence-density=0.25 sequence-density-rank=1 fanout-score=3.18 fanout-score-rank=35 prefix-density=0.31 prefix-fanout=2.6 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.09 sequence-density-rank=20 fanout-score=193.79 fanout-score-rank=1 prefix-density=0.86 prefix-fanout=21.0 sequence=CTTCTTCTTCTG criterion=sequence-density sequence-density=0.67 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=38 prefix-density=0.67 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=23 fanout-score=172.64 fanout-score-rank=1 prefix-density=0.77 prefix-fanout=24.1 sequence=GGCGGCGGCGGAG ERR5262790 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:44:41 Started mapping on | Dec 06 11:44:41 Finished on | Dec 06 11:47:21 Mapping speed, Million of reads per hour | 735.98 Number of input reads | 32710248 Average input read length | 285 UNIQUE READS: Uniquely mapped reads number | 31804370 Uniquely mapped reads % | 97.23% Average mapped length | 284.48 Number of splices: Total | 27807033 Number of splices: Annotated (sjdb) | 25901424 Number of splices: GT/AG | 27429184 Number of splices: GC/AG | 311679 Number of splices: AT/AC | 16530 Number of splices: Non-canonical | 49640 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.41 Insertion rate per base | 0.01% Insertion average length | 2.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 351443 % of reads mapped to multiple loci | 1.07% Number of reads mapped to too many loci | 867 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.68% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 554435 554435 554435 N_multimapping 351443 351443 351443 N_noFeature 1265573 30947370 1556005 N_ambiguous 663886 3957 97849 UnstrandedReadsAssigned:29874911 PositiveStrandReadsAssigned:853043 NegativeStrandReadsAssigned:30150516 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=140 echo kmer=135 ERR5262790 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262790-trimmed-pair1.fastq ERR5262790-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,710,248 reads, 30,434,781 reads pseudoaligned [quant] estimated average fragment length: 239.007 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,188 rounds 52973 ERR5262790.ke.tsv 35125 ERR5262790.se.tsv 88098 total ==> ERR5262790.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 698.769 0 0 PNS24247 1044 805.993 209.867 12.4767 PNS24249 1928 1689.99 450.324 12.7681 PNS24246 1044 805.993 209.867 12.4767 PNS24248 1044 805.993 209.867 12.4767 PNS24244 1471 1232.99 252.073 9.79608 PNS24243 293 117.57 0 0 KQK14069 1603 1364.99 31720.2 1113.5 KQK14071 474 262.914 707.865 129.01 ==> ERR5262790.se.tsv <== BRADI_1g14170v3 35334 BRADI_1g53295v3 245 BRADI_1g59795v3 721 BRADI_1g07683v3 0 BRADI_1g00485v3 62 BRADI_1g20270v3 1462 BRADI_1g74790v3 3316 BRADI_1g09890v3 0 BRADI_1g77505v3 283 BRADI_1g48960v3 0 ERR5262790 completed mapping pipeline successfully