Starting /dee2/code/volunteer_pipeline.sh ERR5262791 current disk space = 1551466254336 free memory = 1604540432 ERR5262791 SRAfilesize e1c9db5841055578d945165fabc881c5 ERR5262791.sra ERR5262791.sra file validated ERR5262791 is paired end ERR5262791 is conventional basespace ERR5262791 read1 length is 101-110 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262791_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 101-110 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.828 37.0 37.0 37.0 37.0 37.0 2 36.708 37.0 37.0 37.0 37.0 37.0 3 36.7555 37.0 37.0 37.0 37.0 37.0 4 36.752 37.0 37.0 37.0 37.0 37.0 5 36.7085 37.0 37.0 37.0 37.0 37.0 6 36.6955 37.0 37.0 37.0 37.0 37.0 7 36.675 37.0 37.0 37.0 37.0 37.0 8 36.7205 37.0 37.0 37.0 37.0 37.0 9 36.719 37.0 37.0 37.0 37.0 37.0 10-11 36.726749999999996 37.0 37.0 37.0 37.0 37.0 12-13 36.75725 37.0 37.0 37.0 37.0 37.0 14-15 36.701499999999996 37.0 37.0 37.0 37.0 37.0 16-17 36.6785 37.0 37.0 37.0 37.0 37.0 18-19 36.585499999999996 37.0 37.0 37.0 37.0 37.0 20-21 36.667 37.0 37.0 37.0 37.0 37.0 22-23 36.6115 37.0 37.0 37.0 37.0 37.0 24-25 36.6425 37.0 37.0 37.0 37.0 37.0 26-27 36.6005 37.0 37.0 37.0 37.0 37.0 28-29 36.558 37.0 37.0 37.0 37.0 37.0 30-31 36.541 37.0 37.0 37.0 37.0 37.0 32-33 36.622 37.0 37.0 37.0 37.0 37.0 34-35 36.633250000000004 37.0 37.0 37.0 37.0 37.0 36-37 36.602000000000004 37.0 37.0 37.0 37.0 37.0 38-39 36.608000000000004 37.0 37.0 37.0 37.0 37.0 40-41 36.62425 37.0 37.0 37.0 37.0 37.0 42-43 36.62525 37.0 37.0 37.0 37.0 37.0 44-45 36.62025 37.0 37.0 37.0 37.0 37.0 46-47 36.571 37.0 37.0 37.0 37.0 37.0 48-49 36.603 37.0 37.0 37.0 37.0 37.0 50-51 36.579750000000004 37.0 37.0 37.0 37.0 37.0 52-53 36.57325 37.0 37.0 37.0 37.0 37.0 54-55 36.599000000000004 37.0 37.0 37.0 37.0 37.0 56-57 36.585750000000004 37.0 37.0 37.0 37.0 37.0 58-59 36.5875 37.0 37.0 37.0 37.0 37.0 60-61 36.6215 37.0 37.0 37.0 37.0 37.0 62-63 36.543000000000006 37.0 37.0 37.0 37.0 37.0 64-65 36.53025 37.0 37.0 37.0 37.0 37.0 66-67 36.54975 37.0 37.0 37.0 37.0 37.0 68-69 36.542500000000004 37.0 37.0 37.0 37.0 37.0 70-71 36.508750000000006 37.0 37.0 37.0 37.0 37.0 72-73 36.4825 37.0 37.0 37.0 37.0 37.0 74-75 36.536 37.0 37.0 37.0 37.0 37.0 76-77 36.591750000000005 37.0 37.0 37.0 37.0 37.0 78-79 36.54 37.0 37.0 37.0 37.0 37.0 80-81 36.487750000000005 37.0 37.0 37.0 37.0 37.0 82-83 36.500249999999994 37.0 37.0 37.0 37.0 37.0 84-85 36.48125 37.0 37.0 37.0 37.0 37.0 86-87 36.526250000000005 37.0 37.0 37.0 37.0 37.0 88-89 36.45075 37.0 37.0 37.0 37.0 37.0 90-91 36.46125 37.0 37.0 37.0 37.0 37.0 92-93 36.443 37.0 37.0 37.0 37.0 37.0 94-95 36.574 37.0 37.0 37.0 37.0 37.0 96-97 36.5 37.0 37.0 37.0 37.0 37.0 98-99 36.49625 37.0 37.0 37.0 37.0 37.0 100-101 36.5345 37.0 37.0 37.0 37.0 37.0 102-103 36.504876219054765 37.0 37.0 37.0 37.0 37.0 104-105 36.472368092023004 37.0 37.0 37.0 37.0 37.0 106-107 36.756878439219605 37.0 37.0 37.0 37.0 37.0 108-109 31.0 NaN NaN NaN NaN NaN 110 37.0 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 2.0 27 1.0 28 6.0 29 4.0 30 15.0 31 16.0 32 31.0 33 42.0 34 71.0 35 183.0 36 2305.0 37 1324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 69.39999999999999 0.0 30.599999999999998 2 36.85 30.599999999999998 29.4 3.15 3 20.525 19.175 17.8 42.5 4 22.1 24.5 19.85 33.550000000000004 5 25.8 35.85 18.025 20.325 6 22.400000000000002 36.375 20.0 21.224999999999998 7 14.7 28.499999999999996 39.025 17.775 8 19.875 24.125 29.225 26.775 9 22.225 20.625 32.574999999999996 24.575 10-11 22.6 32.9875 22.05 22.3625 12-13 21.95 25.7125 26.650000000000002 25.687500000000004 14-15 22.287499999999998 25.75 25.337500000000002 26.625 16-17 23.5625 26.575 25.087500000000002 24.775 18-19 22.5875 28.275 24.212500000000002 24.925 20-21 23.825 24.887500000000003 26.1125 25.174999999999997 22-23 22.1875 27.487499999999997 26.237500000000004 24.087500000000002 24-25 23.5875 27.125 23.5375 25.75 26-27 23.825 26.2875 23.825 26.0625 28-29 22.412499999999998 28.3625 24.0375 25.1875 30-31 23.1 27.775 25.224999999999998 23.9 32-33 22.6125 27.6875 24.925 24.775 34-35 21.1125 27.375 24.9375 26.575 36-37 23.200000000000003 27.8875 23.8375 25.074999999999996 38-39 22.75 27.1125 24.224999999999998 25.912499999999998 40-41 23.5375 28.175 24.6875 23.599999999999998 42-43 23.3875 26.474999999999998 24.7375 25.4 44-45 22.8625 26.187500000000004 24.675 26.275 46-47 23.400000000000002 27.6625 25.4375 23.5 48-49 23.474999999999998 26.075 25.587500000000002 24.8625 50-51 22.925 26.487500000000004 26.35 24.2375 52-53 23.150000000000002 26.2875 24.6625 25.900000000000002 54-55 22.7 25.6125 26.075 25.6125 56-57 23.3 25.8 25.224999999999998 25.674999999999997 58-59 21.912499999999998 26.625 25.887500000000003 25.575 60-61 23.549999999999997 26.4125 23.962500000000002 26.075 62-63 22.900000000000002 26.2125 24.25 26.637499999999996 64-65 23.2625 25.9625 25.912499999999998 24.8625 66-67 23.325000000000003 27.237499999999997 24.6625 24.775 68-69 22.75 26.525 25.374999999999996 25.35 70-71 24.5125 25.412499999999998 24.45 25.624999999999996 72-73 22.412499999999998 25.587500000000002 26.6625 25.337500000000002 74-75 24.7875 25.724999999999998 24.587500000000002 24.9 76-77 23.8875 26.187500000000004 24.587500000000002 25.337500000000002 78-79 23.35 25.575 23.9875 27.0875 80-81 24.95 25.0625 25.3 24.6875 82-83 24.575 25.7 24.712500000000002 25.0125 84-85 22.287499999999998 26.3125 25.2875 26.1125 86-87 21.9625 24.875 26.950000000000003 26.2125 88-89 23.175 23.8375 26.1125 26.875 90-91 21.912499999999998 26.937499999999996 25.637500000000003 25.5125 92-93 24.474999999999998 25.25 25.5375 24.7375 94-95 25.3125 27.8875 22.85 23.95 96-97 22.55 23.45 26.924999999999997 27.075 98-99 28.1875 26.875 22.7375 22.2 100-101 22.162499999999998 28.262500000000003 27.025 22.55 102-103 21.117779444861213 20.64266066516629 32.12053013253313 26.11902975743936 104-105 19.91747936984246 31.45786446611653 25.268817204301076 23.355838959739934 106-107 27.99203782035332 7.041552625031103 25.3048021895994 39.661607365016174 108-109 50.0 50.0 0.0 0.0 110 0.0 100.0 0.0 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 2.5 29 4.0 30 4.5 31 11.0 32 22.0 33 34.5 34 36.5 35 46.5 36 74.5 37 77.0 38 93.5 39 114.0 40 124.0 41 167.5 42 202.0 43 213.0 44 217.0 45 213.5 46 224.5 47 225.5 48 176.5 49 161.0 50 167.5 51 144.5 52 146.5 53 126.0 54 94.0 55 75.0 56 71.5 57 82.0 58 61.0 59 39.5 60 53.0 61 66.0 62 56.5 63 40.0 64 31.5 65 45.5 66 42.5 67 43.0 68 42.5 69 22.5 70 20.5 71 17.0 72 11.0 73 11.5 74 10.5 75 9.0 76 11.0 77 10.0 78 2.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 101 1.0 102 0.0 103 0.0 104 0.0 105 1.0 106 3977.0 107 20.0 108 0.0 109 0.0 110 1.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.550000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 40.88913282107574 18.625 2 28.48518111964874 25.95 3 14.763995609220636 20.175 4 8.89132821075741 16.2 5 3.951701427003293 9.0 6 1.481888035126235 4.05 7 0.27442371020856204 0.8750000000000001 8 0.6037321624588364 2.1999999999999997 9 0.49396267837541163 2.025 >10 0.1646542261251372 0.8999999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTACGAGTGATAGATCATTAAGCGGAATACAAGCAAAAGGTAGTAC 15 0.375 No Hit AGAAGAACACAAGCGTAGTCGTCGTAGTAGAAATCAAAGACTTGGACACG 11 0.27499999999999997 No Hit ATCCATCCAATCCATGGCAGTCGATGCATATCCACTGTACTCTCATGGAT 10 0.25 No Hit ATTATCTTTCTTACCTAATGAATAAGATCCAAGCATGTAATCTTCATCTT 9 0.22499999999999998 No Hit ATCCACAAGTCCACCGACCCTTCGACTTGAAGAATTCGTCGTTCCGATTA 9 0.22499999999999998 No Hit CACTAAGCCAAACGCCCCTAAGGCAGCCCTAGACCTAGCCCTACACCAAA 9 0.22499999999999998 No Hit CACCGATCTCTCTCTCTCGCTCACGCTCAGATCTGCGCCTGCGGATGTGG 9 0.22499999999999998 No Hit ATGGGAGCGAGCGCGGCATAGCGGCGGCAGCGCACGGCGAGTATGTGAGG 9 0.22499999999999998 No Hit AGACAGATGGATCCACGAGATCGACTGAAAGTCTTATTCTTGTCTTATGA 9 0.22499999999999998 No Hit ATCATTTTGGATCCAAAACCCTTCGTATGCCTCTCAAAAGTGCCGAGGCT 9 0.22499999999999998 No Hit CAATTCAATTCGGATCCAAACCGATTCGTTCCTCCAAATCCCTACTCAGT 9 0.22499999999999998 No Hit ATTACAAATAGTGAATGGTCCACAAACAGCAAAGACATCACACTGAACTC 9 0.22499999999999998 No Hit CACGGTAACACTCGTTGAGCCTTTCTCTTGCATCCCTAAGAAGAGACCAA 8 0.2 No Hit CACTGATCTTCTTCTTGTCCACAATTGCCTTGGCTCCATGGAGTAGTATG 8 0.2 No Hit AGACGACAGTCAATAATATACATACAATAAGAATAAGAAACAAAAACACA 8 0.2 No Hit AGGGCATCCCCATGTGGAGCTTCCCCAAATCAATGGTCCACTCCTCATAA 8 0.2 No Hit AGTCCATGCTCGTCCCCGGTGGGGAGGTTGTCGACGTAGCTGATGAGCAT 8 0.2 No Hit CAAGTGAGGCCCTCCATTTGAACACTGCGGACAGCCTCCTCCAACTTCTT 8 0.2 No Hit ATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAGAC 8 0.2 No Hit AGCCACAGTTGCAGCTTGATCCACAGCTGCAAGACATCTTCCAAATCTCT 8 0.2 No Hit AGTGAATTTGTTCCATCCCATGCTGATTATTTGCAGCATCGGGAGACTGA 8 0.2 No Hit CACCACTACTACTGTATAGCGTACCCATCTGAGCAGTACCGTATTTGATA 8 0.2 No Hit CACCTCCACAGTTGCAGCAACAACAGGGCCTTTCCCACTGTCGCTACCAT 8 0.2 No Hit CACCAACTTGGAAGTACCAGGACTTCTATCCAGTTCGATTACCTCTGGTT 7 0.17500000000000002 No Hit AGTAGATGTCATTCTGTCCCTCCTTCATCCTGGTCACATAGTCCTTGAGG 7 0.17500000000000002 No Hit AGGGCATTTAACTTCGCAATCTTAACAAGTTGATCATGGCCATAGAAAAA 7 0.17500000000000002 No Hit CACTGGCTTGCTTGTGTGGAGAGCTGTCATAGAAGTCCCGGCCACCGTAA 7 0.17500000000000002 No Hit ATTGCCAGGACCTCCCCTCGCACAGATTCTGGCAATATGCATGGCATAGC 7 0.17500000000000002 No Hit AGCATAATGTTTTCCTTCACCAGCTCCAACATCAGTAATGTGTCCAGTAC 6 0.15 No Hit AGCCAATCCACCAGCAGAGCAAACAGGATTGCCACCAAATGTGTTAAACT 6 0.15 No Hit AGAACAAGTCTCAGGGAGAGAAGGAGCAGCATGGATAAGCTCATTTCGCT 6 0.15 No Hit AGCTTCCACCCACCGATGCAGCTTCATCTGATTCTGAATCGGTAGTAGTT 6 0.15 No Hit ATTGCCTTGATGTATTTGGCATACTTACTTTATACTGCCGTACACTTTTG 6 0.15 No Hit CACCGGTCTTAACCCCAAATACCAGCCTGCGGAGTCCTACGACCATTTCT 6 0.15 No Hit ATTGGCGCCTGCATACTCCTTGTAGGGAAAGGCAAATAGGTGCCCAATGG 6 0.15 No Hit AGCTTTTCGAGGCCCGTCTCCGGGCGAGGCCCGGCTACCACGTCGTCCCA 6 0.15 No Hit ATCCATCAAAGCTCTGCCACTCTCTGGGAAAGTAGGCTTCCTAGGTACTA 6 0.15 No Hit CACGCATGGATTTGTTGACCTCAGAGGAGCCTAACTTGCATCCCCGCTGC 6 0.15 No Hit ATCCACGGCCTCGGCGGTCTTCCTGGCGGACACGAGGCCCAGGGAGTTCA 6 0.15 No Hit CAACAGATATTTCCTGAAACCCAAGCCCAAGGATGCCATCGAACTTTGCA 6 0.15 No Hit ATCCATTCGTGCGTGCTGTGCTCATCAAAGCACGGTCGCCAAGATTGTCA 6 0.15 No Hit AGACATCATCATTCATAAACAGAGAGTGCTGAATTTAACATAAAGGTACC 6 0.15 No Hit CAATCTTCTCCAACTTTGAGCATGCCGTAGTAAATGCTTTTGATATGTGG 6 0.15 No Hit ATCCAGAGCAAGAATGGAAATATGTTTGGCTACCCTGCCAATTGCATGCT 6 0.15 No Hit CACGTACATCGTCAGCAACTTTCATGTCGAGATTAGGGATACCATCGGAT 6 0.15 No Hit CACCATTGTTAATGATGAACTCAAATGCATAGTCCATCAGACCTCCATTG 6 0.15 No Hit CAAAAAGTTAGAGAAGTACATTATTGCGTCTAACAAAGAGATGCTGCACA 6 0.15 No Hit ATCAGATGGCAGTTCAAACCGGCAAACCGGGCAGGAACTATGGAGTTCAA 6 0.15 No Hit ATCCATTCCATCACACTCTTGAGGCACTAGCACAAACCAACTCCAAGTGC 6 0.15 No Hit CAGAACAATATTTAGAACAGTATAAATTGCTGAAAAGAGGAGCAACATAT 6 0.15 No Hit AGCCATGTGAGATTCGTACTGACCAACAGTGTCCTTGTTTCCAGCAATCT 6 0.15 No Hit AGGCAATTGCAGCTGCAGTTGGCTCGTTGATGATACGCATGACATTAAGA 6 0.15 No Hit CAACGAAATTGAGAGGCGTGCATGCCACTGTATAAGGACGAAGCGAGTAC 6 0.15 No Hit CAACCCAGCTATAAATCCCATTCCCTGCACATATCCAACGTCTCTATCAT 6 0.15 No Hit AGTACAGCGTGAAGATGAGCGCGGGCTCCGTCCAGAAGGACACGTACGGC 6 0.15 No Hit AGCGGGTACACCTCGGGCCGGACCCATTTGCTGGCGGCCATCGGCGTTGC 5 0.125 No Hit CACTTGTCAAGGCTTTTATTAGGTACATGCTCAAAGCAGAGCAATCTTTT 5 0.125 No Hit AGCAGTTTCATGTTCCGGCATTCATAGTTATCACCTCTTTGGTTGGGAAC 5 0.125 No Hit CAACAAAATATAAGGATTCTTAAAATTAAACCATCCATGTTCACCAGAGC 5 0.125 No Hit CACTCGTCGAAGTTTGCGATGCAGGTCTGACCAGCAACATTAGGGCAGAA 5 0.125 No Hit CACTAAAGAAACAAAAAGAAGAATCTCAAAATTACATCCTGTACACACTA 5 0.125 No Hit ATTCACTGGCCAGTTAAAACGGAAGTGATTCAAGAGCTGAAATCTTTCCA 5 0.125 No Hit ATTTCATGCATGGGTCTGTAGAGAGATCGAACATTATCGGAGGTGCTATC 5 0.125 No Hit AGCTCCTTAGCATTCATGATGGTTGAGTTCACAAAAACGGAGGCAGCCAA 5 0.125 No Hit CACGGACAAACACCTAATGGTAACCCTTAAACATCTCAAACCCTACGCGA 5 0.125 No Hit CACCAGATGTTGATTCTGTGCTAACAAGGTGACGGTAATCAATGTCCATA 5 0.125 No Hit ATGTAAAGCGACTATAGGCATCAATGAAGCTGACATAATAGTTGTGACCA 5 0.125 No Hit AGGAAGATCCCGATCAAAGCTACAATTAAGACAATTACAAAGGAAAACCC 5 0.125 No Hit ATCAGAAGTAGAGTCAAACTTAATGCCTCCACCGGTACGGGACCCAGAGG 5 0.125 No Hit AGATAGATAACCATAAAGACCAACAAACATGGCGTCGCAGAAGTTGTTCA 5 0.125 No Hit ATGTACACAGCAGGATGTCATGTCAGTGAGATAACAGAGATTGCAATGTC 5 0.125 No Hit CAACAAAATCAATCACAGGCAAAGGCTCGATGAATGCTGTAGATGACATA 5 0.125 No Hit AGCCAGAGCCGACTCCAACACTTGGAGCATGAGCATCACCTGCAGCTGAA 5 0.125 No Hit ACTGAATCGAGGGTACAAAGGCATAGAGGAAAACTGTCTGAACGCATCAG 5 0.125 No Hit ATGAATATGATCCTCTCGCGCTCTCGATCTCCTTTTCAAAGTTAATTTTT 5 0.125 No Hit CAATCCGTAGCGGCTCGCGTTCGCCCTCTTGATGACCTCGTTGAGATCAT 5 0.125 No Hit ATTTGTATCTCTCCCAGCATTGGCCATGGAGAGAAAACCTGGTCCAGTGT 5 0.125 No Hit CAAAAGAATTAAACTTACAATCCGGTAGTCCAGAAAACAATATCGCAGAT 5 0.125 No Hit CACGGAGGCAGGTCTTTCTCAAACTTCCCAGCCAAAGCATCAGCCAACAA 5 0.125 No Hit ATCCAATGTTAGCCCAGCTGCAGACATGAAGCCAAGGGCTTCATCAACCC 5 0.125 No Hit CAAGGGAGAACGGTAATAGGGGACTCAAACGAAGCAGAGTTACAACCTTG 5 0.125 No Hit CAGAAATAAACCTTGCCCGGGACAAATAGCTTCCAACATACGAAACTGCA 5 0.125 No Hit AGATGACTCCACCTCAAGGGTGATGGTCTTGCCAGTCAAAGTCTTCACAA 5 0.125 No Hit CACCCGTCGGCCCAGAATCAGCAGCAGCGTGATCGTCATCATTACCGCCA 5 0.125 No Hit ATCCCCTTTAACTTCAGAAACCTCTAACCAAACTGAAGTAGTCTCACTCC 5 0.125 No Hit AGGCTGCCGGCTCCTCGCTCTCGCTGCATCACCACCACCAGCACTAGTGT 5 0.125 No Hit AGTAGGTAGGAAAAGGCCAACTGCCAGTCACCAAGCTCTCGCTTCCGTGG 5 0.125 No Hit AGTACGACGATTTCACAGCAATTAGGCTGCCGACAATTTCTCGCAAACAG 5 0.125 No Hit AGAAATTTCATTATATCAACCAATACTAGTGACTTTGGCCATCTAGCTTT 5 0.125 No Hit ATTTTGCCATGTTGATTTTGCCATGTCATTGCCTGACATGATTTTACCAC 5 0.125 No Hit ATTGCGCTTGGGGAAGATGGGGGCCGAATCAGAGCGGCCGCGGAGCCCAG 5 0.125 No Hit ACTGGAACTTCCTTCTCTGGCCGGATATATCCCTCCCTCCAATGGAACTG 5 0.125 No Hit ATGGGGTTGGCGGAGACAGTAGCCACAACAGTTTCCGCAATGATGATGGT 5 0.125 No Hit AGAACCAATATTGTGTTCACCATATTGTTTGTACCTAAGATTGCACAAAA 5 0.125 No Hit ATGGCGATGAGCGCGGAGCTGTTGGCCGGGATATTGGCCCTCCCCGCCAT 5 0.125 No Hit CACTGGATAAAATGCATCTGCCTCCATATAGAGCTAATCCTCATCAAACT 5 0.125 No Hit ATGGGACTTGCTGCATGATGCGGAAAATATACATAGACTGACTTCACGTC 5 0.125 No Hit ATCATTTACAGAGGGTATTTTGTAGGAACCCGGACCACAGGTGAATAGGT 5 0.125 No Hit CACCAACATCGAAGCGGGGACTGATGACTCCACACTTGTTCAACCTGCCG 5 0.125 No Hit ATTTTGTTTGTTCAAGGCTCATAAGGCCTGTTAGTAGATTTTCTATATTC 5 0.125 No Hit AGCCAGCGCATGAAGTAACAAGGGACCGAGAACAACTGCACATGCTCTTC 5 0.125 No Hit CACCATCTCAGTTTGTCCGGGAAGTACTTCTCGATAAGTGACATGTAGTA 5 0.125 No Hit CACTTGGGGGCCAAGGCGGGTACCTGTGGTAGTACGGCGGTGGCAGCCCA 5 0.125 No Hit CACCGTGATAAATTCTGATTCAGTGTCAAGTAGTGCGACTGCTTTGCCAT 5 0.125 No Hit CACCATTCAGTACTGAGCCAATAACTGTTAAAAGAGAATTTCCGTAGCAG 5 0.125 No Hit CACCAGAGTTGCCAATGCAAGTGGTACATCCGTATCCAACAAGATGGAAT 5 0.125 No Hit ATCAGCAGCAGCATCTCGACGAGCCTTCTCGCGCTCAAGACGTTTCTTTG 5 0.125 No Hit AGAAAACTAGACCGGAAAGACAAAAGAACAGAATCATCAGTGTGTTCCTT 5 0.125 No Hit AGGCAGTACGGCCATGCTGCGGGTTCTTAGTTCCATCCATGGCCAAGATC 5 0.125 No Hit AGACACACGGTACATCGATGCAGGCGGTAAATTACCGAAATGAAACACTT 5 0.125 No Hit CAACAAGAACATGTTCACAGACCCAAAACGTGTAGTTTGTACACAAAAAA 5 0.125 No Hit CACGAACAGCTCACCGCACGCATCGAGAATTCGAGCCCTCCGCGGCGGAG 5 0.125 No Hit ATGCCACCTTCAGTTTTCAGTTCCCAACCATTCACCTGATCTAGCCACTT 5 0.125 No Hit AGGGCGTCTTGCGCAGCTTGTGCTGCATGTGGATCTCGCTGGTAGCGATG 5 0.125 No Hit AGCAGGTGTTTGTCTTCAAGATCATCATATTCCAACTGCTCGTTGCTGGT 5 0.125 No Hit ATGGGTTCACGAAACTAAACGATGAGACGACGAAACGGAGGGCATTGACG 5 0.125 No Hit ATGCATCATTACACCTGTACAAACCTGTACGGTATCTGCGCCTAGTAGAA 5 0.125 No Hit AGTCTGCTTAGTACGAGCCATGAGAAAACTTCGTCTTACCTTGCGCGCTT 5 0.125 No Hit AGGGAACAATCTTGAAAACCTTCATCTTCTCGATCCATGTGTTGAAGGAG 5 0.125 No Hit CAAATTTATTCAGATACCAGTATATTCCACAAAAACAATTTGTCTGGAAT 5 0.125 No Hit AGACCCTAAAGCAGAAACAAAACATACTAAAGATATCCTTGGTTAAATAG 5 0.125 No Hit CACTTTACCAAATTTCTTTATAGCCTTTGTTGTACTGCTCGAGCGTTCAA 5 0.125 No Hit CACCCTTGATCTCGGAAATCCATTGCCCAAGTAGCAATGCAACTTTTCGA 5 0.125 No Hit AGGAACACACGACAGGTAGCATCACGGACAAACACCTAATGGTAACCCTT 5 0.125 No Hit AGAAAAGAAAAGAATGGGTTAACGCCAAAAATAATCAACAGAAAAGATCA 5 0.125 No Hit AGGACAACAGAGCCAGCCTCCATGGTAATTGGTCTTTGAAGATTCTCGAT 5 0.125 No Hit AGGGATTTCTCGACGCGGCGCGGCTCGGCGCGTCTGTTTGGTTTGCGTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR5262791 read2 length is 96-110 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262791_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 96-110 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.511 37.0 37.0 37.0 37.0 37.0 2 36.238 37.0 37.0 37.0 37.0 37.0 3 36.386 37.0 37.0 37.0 37.0 37.0 4 36.5075 37.0 37.0 37.0 37.0 37.0 5 36.585 37.0 37.0 37.0 37.0 37.0 6 36.579 37.0 37.0 37.0 37.0 37.0 7 36.5875 37.0 37.0 37.0 37.0 37.0 8 36.659 37.0 37.0 37.0 37.0 37.0 9 36.684 37.0 37.0 37.0 37.0 37.0 10-11 36.64 37.0 37.0 37.0 37.0 37.0 12-13 36.684 37.0 37.0 37.0 37.0 37.0 14-15 36.61375 37.0 37.0 37.0 37.0 37.0 16-17 36.61875 37.0 37.0 37.0 37.0 37.0 18-19 36.596000000000004 37.0 37.0 37.0 37.0 37.0 20-21 36.62825 37.0 37.0 37.0 37.0 37.0 22-23 36.56175 37.0 37.0 37.0 37.0 37.0 24-25 36.594750000000005 37.0 37.0 37.0 37.0 37.0 26-27 36.573 37.0 37.0 37.0 37.0 37.0 28-29 36.6055 37.0 37.0 37.0 37.0 37.0 30-31 36.557249999999996 37.0 37.0 37.0 37.0 37.0 32-33 36.53125 37.0 37.0 37.0 37.0 37.0 34-35 36.545500000000004 37.0 37.0 37.0 37.0 37.0 36-37 36.507 37.0 37.0 37.0 37.0 37.0 38-39 36.49925 37.0 37.0 37.0 37.0 37.0 40-41 36.5235 37.0 37.0 37.0 37.0 37.0 42-43 36.4735 37.0 37.0 37.0 37.0 37.0 44-45 36.457 37.0 37.0 37.0 37.0 37.0 46-47 36.53375 37.0 37.0 37.0 37.0 37.0 48-49 36.51675 37.0 37.0 37.0 37.0 37.0 50-51 36.524 37.0 37.0 37.0 37.0 37.0 52-53 36.46225 37.0 37.0 37.0 37.0 37.0 54-55 36.4765 37.0 37.0 37.0 37.0 37.0 56-57 36.45975 37.0 37.0 37.0 37.0 37.0 58-59 36.473749999999995 37.0 37.0 37.0 37.0 37.0 60-61 36.482749999999996 37.0 37.0 37.0 37.0 37.0 62-63 36.39775 37.0 37.0 37.0 37.0 37.0 64-65 36.459 37.0 37.0 37.0 37.0 37.0 66-67 36.4835 37.0 37.0 37.0 37.0 37.0 68-69 36.42775 37.0 37.0 37.0 37.0 37.0 70-71 36.37225 37.0 37.0 37.0 37.0 37.0 72-73 36.408249999999995 37.0 37.0 37.0 37.0 37.0 74-75 36.46625 37.0 37.0 37.0 37.0 37.0 76-77 36.363 37.0 37.0 37.0 37.0 37.0 78-79 36.367999999999995 37.0 37.0 37.0 37.0 37.0 80-81 36.3795 37.0 37.0 37.0 37.0 37.0 82-83 36.29925 37.0 37.0 37.0 37.0 37.0 84-85 36.31125 37.0 37.0 37.0 37.0 37.0 86-87 36.31425 37.0 37.0 37.0 37.0 37.0 88-89 36.32575 37.0 37.0 37.0 37.0 37.0 90-91 36.35225 37.0 37.0 37.0 37.0 37.0 92-93 36.3565 37.0 37.0 37.0 37.0 37.0 94-95 36.36575 37.0 37.0 37.0 37.0 37.0 96-97 36.316163228307076 37.0 37.0 37.0 37.0 37.0 98-99 36.32483120780195 37.0 37.0 37.0 37.0 37.0 100-101 36.18734367183592 37.0 37.0 37.0 37.0 37.0 102-103 36.32440550688361 37.0 37.0 37.0 37.0 37.0 104-105 36.34142678347935 37.0 37.0 37.0 37.0 37.0 106-107 34.92212127715382 37.0 37.0 37.0 37.0 37.0 108-109 24.0 NaN NaN NaN NaN NaN 110 25.0 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 0.0 21 0.0 22 2.0 23 2.0 24 0.0 25 5.0 26 6.0 27 5.0 28 7.0 29 4.0 30 3.0 31 18.0 32 27.0 33 31.0 34 68.0 35 295.0 36 2518.0 37 1007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.525 25.6 6.875000000000001 28.000000000000004 2 26.35 25.3 31.3 17.05 3 20.5 25.05 31.574999999999996 22.875 4 25.45 31.25 20.875 22.425 5 26.5 33.15 20.424999999999997 19.925 6 23.075000000000003 36.05 19.875 21.0 7 22.05 18.224999999999998 36.275 23.45 8 21.95 22.85 25.074999999999996 30.125 9 22.325 22.95 28.625 26.1 10-11 27.075 26.775 23.575 22.575 12-13 23.95 23.0375 27.825 25.1875 14-15 24.725 25.5 25.25 24.525 16-17 25.4875 25.587500000000002 26.924999999999997 22.0 18-19 26.825 26.2625 23.8125 23.1 20-21 26.1625 26.987499999999997 24.8625 21.987499999999997 22-23 26.2625 25.35 26.275 22.112499999999997 24-25 25.05 24.65 25.650000000000002 24.65 26-27 24.7375 25.45 25.074999999999996 24.7375 28-29 27.0 23.974999999999998 25.525 23.5 30-31 25.2625 24.6875 26.075 23.974999999999998 32-33 24.962500000000002 24.587500000000002 25.724999999999998 24.725 34-35 25.3125 26.7625 25.587500000000002 22.3375 36-37 25.637500000000003 24.525 25.324999999999996 24.5125 38-39 25.35 25.5 26.4625 22.6875 40-41 24.837500000000002 24.712500000000002 27.125 23.325000000000003 42-43 24.837500000000002 26.0 25.9625 23.200000000000003 44-45 26.687499999999996 24.3875 26.2125 22.7125 46-47 26.05 23.95 26.4125 23.5875 48-49 25.5625 26.0125 26.525 21.9 50-51 25.674999999999997 25.224999999999998 25.7875 23.3125 52-53 25.6125 26.1125 25.650000000000002 22.625 54-55 25.874999999999996 24.55 26.387500000000003 23.1875 56-57 24.2375 26.525 26.2875 22.95 58-59 24.8625 25.5125 26.087500000000002 23.5375 60-61 23.325000000000003 25.687500000000004 27.762500000000003 23.225 62-63 26.387500000000003 24.775 26.05 22.787499999999998 64-65 25.25 24.825 26.5125 23.4125 66-67 23.7625 24.7 28.1375 23.400000000000002 68-69 25.687500000000004 24.2875 27.150000000000002 22.875 70-71 25.2625 23.6875 27.800000000000004 23.25 72-73 26.4625 25.137500000000003 27.237499999999997 21.1625 74-75 24.7375 25.074999999999996 27.750000000000004 22.4375 76-77 23.974999999999998 25.162499999999998 27.6875 23.175 78-79 25.1 24.1375 28.349999999999998 22.412499999999998 80-81 26.174999999999997 23.9375 26.237500000000004 23.65 82-83 25.674999999999997 23.7125 26.987499999999997 23.625 84-85 24.1375 26.2625 27.375 22.225 86-87 25.124999999999996 26.187500000000004 24.85 23.8375 88-89 24.837500000000002 24.212500000000002 28.4 22.55 90-91 24.825 25.124999999999996 26.4625 23.5875 92-93 26.6 25.412499999999998 25.7375 22.25 94-95 25.674999999999997 26.6 25.75 21.975 96-97 22.30278784848106 22.26528316039505 32.89161145143143 22.540317539692463 98-99 25.70642660665166 30.932733183295824 22.230557639409852 21.13028257064266 100-101 19.484742371185593 29.65232616308154 32.416208104052025 18.44672336168084 102-103 26.9837296620776 18.89862327909887 30.125156445556943 23.992490613266586 104-105 22.81602002503129 23.717146433041304 24.84355444305382 28.623279098873596 106-107 30.510585305105852 0.099626400996264 69.1656288916563 0.22415940224159403 108-109 0.0 50.0 50.0 0.0 110 0.0 100.0 0.0 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 0.5 26 0.5 27 0.0 28 1.5 29 4.0 30 5.5 31 7.5 32 14.5 33 31.0 34 47.0 35 49.5 36 73.5 37 97.0 38 90.0 39 97.0 40 130.0 41 162.0 42 182.0 43 222.0 44 227.0 45 208.5 46 212.5 47 190.5 48 188.0 49 193.5 50 153.0 51 136.5 52 151.0 53 124.0 54 107.5 55 102.0 56 72.0 57 59.5 58 64.0 59 66.0 60 48.0 61 39.5 62 39.5 63 28.0 64 49.5 65 53.5 66 45.0 67 39.5 68 26.5 69 36.0 70 33.0 71 15.5 72 13.0 73 18.0 74 10.5 75 6.5 76 8.0 77 9.5 78 5.0 79 2.0 80 1.5 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 96 1.0 97 0.0 98 0.0 99 1.0 100 0.0 101 3.0 102 0.0 103 0.0 104 0.0 105 1.0 106 3973.0 107 20.0 108 0.0 109 0.0 110 1.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.025 #Duplication Level Percentage of deduplicated Percentage of total 1 42.15100488864748 19.400000000000002 2 27.48506246605106 25.3 3 15.046170559478544 20.775 4 8.365019011406844 15.4 5 4.1281912004345465 9.5 6 1.249321021184139 3.45 7 0.32590983161325365 1.05 8 0.5975013579576317 2.1999999999999997 9 0.48886474741988045 2.025 >10 0.16295491580662683 0.8999999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCTACTACTATCGTTCGTGTTTCCAGATGTTTTACTCCGTGTGGAGCA 15 0.375 No Hit GACGGCTACATCGTGTATGTCTACGTCGGCTACTTCCTCTACATCGCGAC 11 0.27499999999999997 No Hit GATAAGTCGTGTGGGATGAAGGTTGCGAACCGGCTCAACGTGAAGCCGAG 10 0.25 No Hit AGGTCACATGGATGCAAGACTAGTAGTACCAACTAGTGTGTCGTTTCAGT 9 0.22499999999999998 No Hit GTTGCTTCTGCCTCCGACCACGCCGCCGACGACGAGCGCAACCTCACGAG 9 0.22499999999999998 No Hit AACCGAGTAAAGCAAGGTCAGAACAGAGGCGCAACATACGACCAGCAGAC 9 0.22499999999999998 No Hit GACCTTGTGATTTGTGATCGTAGGTTTTGCACTAAGGCTTATCATCCTGG 9 0.22499999999999998 No Hit AATGACTGTATAATCCGTGAACCAATGGTTCAGATCCGTCGTCCTTCGTG 9 0.22499999999999998 No Hit CCCATACAAGCCTGCTGTCAGGCGCAAGGCTTCTTTAATATTTGATAGTG 9 0.22499999999999998 No Hit CTGGAGTGGGGATGGGAGAGGAGGCCGGGCCGCTGGCGTCCGTCGTAGTT 9 0.22499999999999998 No Hit GTCATTTGTTTGGTTGGAGAGCTCACAGGATTGGGTGATGACCTACGCCC 9 0.22499999999999998 No Hit CAGCAGAGCTGCGCCAAGTAGAACAGAGAAGCAAAGCGAAAAGCGAATTA 9 0.22499999999999998 No Hit CAGCCGCCCTTCGTATCACTGATGGTGCTTTGGTGGTGGTTGACTGTATT 8 0.2 No Hit GGTCATGAAGGAGGAGCAGGCCAAGCAAGCTGCCTCCTCCTTCGATAGCA 8 0.2 No Hit GACGCACTGGCACAGGCTTTGATGGACACTAGATTCCCGACCGAGACGCT 8 0.2 No Hit ACAACAGTTTTATCCAAATGAGCCCTCCGAGTCACCTTATTTTGGCTCGT 8 0.2 No Hit ATTGAAAATATGTTAATGAACACTTCCAAAAAGCCAACTGCAGATGAGCT 8 0.2 No Hit GGGATTCTTCCTCTCAGCACACTGCAACATGGATCAGCAAAGCGCTTTCC 8 0.2 No Hit CCATGGTGGAGGAGATGGAGCGCGGGCTGCGCGCCGACCCCCACGCCCCG 8 0.2 No Hit AATCTTTCTCAGCAGAAACTACAACCTAACTGGCTCTATCCCTGACAATG 8 0.2 No Hit GTACATTATGTATTGTACGGGGCTTGTTTGTGTGTGTTTGCGACCGCTGA 8 0.2 No Hit GTGGAAAATCATCTGTGCTGATGGATGTCAAGCCATGGGATGACGAAACT 8 0.2 No Hit GTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATTTCATCAGCTTGA 8 0.2 No Hit GTTAATTTGGTAGCATGAACGGCTGACTCTGACTGCAGATATTCCACAAG 7 0.17500000000000002 No Hit ACCACCTCTCTGTCGGTATGCCTCTCAATTGTATCAACTCAAAGTGATTG 7 0.17500000000000002 No Hit AGAAGATGGACTACCTTCGGAAGCAGGAACGTGATTACCAGCAGCGTGTC 7 0.17500000000000002 No Hit ACCAAGTGTTGCATATGATGGGATGCCTGAAACCCCTTGTTCAGAAAAAC 7 0.17500000000000002 No Hit AAGATTGCTGAGCTGCTGAGGTACCACTCCACTAAGAGTGGTGATGAGCT 7 0.17500000000000002 No Hit GATAGCATGAACAAGAGCAAAGAACATGGATTCCTTGGTTTCCGGAACAC 7 0.17500000000000002 No Hit GCCAGCACATGCAAATTCAAGATCGACTCATCTTTGCCGTTTTCATCTAT 6 0.15 No Hit CTTGTGTGGATGAAGGTTTGCTGTTAAATTGGTCCGGCGGAGCGATTCTA 6 0.15 No Hit TTCTACCCTGTAATAAAGCAAGTATACGACATATTTCATGACAGGCCATA 6 0.15 No Hit GTCAAAGATCAGGAGTTTATTGAAGCTACAAAGGAGCCAGGTGTTACTTT 6 0.15 No Hit CTCGTCGCCGACACCTTCTTCGTGTGGCCGGCCACGCTGGCCCGGAAGCT 6 0.15 No Hit CAGCGCCGGGTGCAGCGTCATGTGATGGCGCCGCGCGTCGACCGCGACGG 6 0.15 No Hit CACTGACTTGATTGCGATGAACCATGCTGACTTCATCATTACCAGTACCT 6 0.15 No Hit TACCAGGCGCCCCAGCTTTGCAATGTTTTTGCCTCCCGCATAAGTAGGGC 6 0.15 No Hit CCATCAAGCAGCTGATGACAACAGGGGGCCAGCACCGTTTGTTGAGATTC 6 0.15 No Hit CACTACAAACCGAGTCATGACGGTTTCTTTCCACAAGTATGGGGACTTCT 6 0.15 No Hit AGCGAGGTGATGGATGACATGGTGGGGAGCACAGGATATAACTTCCCAGG 6 0.15 No Hit GCTCCTGCATCGACCTAGATCTCCATCTCCTTGAGTCCAAGATGCTGGAT 6 0.15 No Hit GTCTGAAGCTTTCCTTCATTCCGTAGCAAATGAAATCCAGCTTAAGCAGT 6 0.15 No Hit GAGACATGGGACAGGTCAAAGATCCCTGTATAATGTTTTAAAAGCGTACT 6 0.15 No Hit CTAGCAGCTAGGTTAGGCTCTTTCACATTCAGACGGACGTCATCTGGCAG 6 0.15 No Hit GTGTATATATGATTGCTGAACTGTAGTGTGCCCAGAATCCCAGATGTTGT 6 0.15 No Hit GGCTAAGAAGATGCCTTGTGAACATAAGTTCCACTCTTCATGTATCCTGC 6 0.15 No Hit GCTTACTTCAATGATTCCCAGAGGCAGGCAACTAAGGATGCTGGTGTTAT 6 0.15 No Hit GCAGAATGAACCATGTTAATGTTGTGGCAAGTAAATAGGGGTAGAAATAG 6 0.15 No Hit GAAGAACACCAAATTGCAGGTAGGTTCATCGATCGAATCGAAGGAACAAA 6 0.15 No Hit ATCGCTCTGTCCGAGCAAGAGCTAGTTGATTGTGACACATCATACAACCA 6 0.15 No Hit GAAATAACAAGGAAATGTTGAACGATGTCTTCGCGTGCTAGAGAGCCACC 6 0.15 No Hit CTTGGGTGCAGTTGTCACTACTCCCGAGATTGCAAACGTAATGGCTCAAA 6 0.15 No Hit GCACCTCTTGAACTTATATTTTCGGATGTGTGGGGTCCAACCCAAACTTC 5 0.125 No Hit CGCCAAGGCTTTGAAGCCAGGAGACACAATATTTGTGGGGCAATATTTGT 5 0.125 No Hit ACCGGAAGCACCGGCAGCTCCAATCCGCCGCGGCCTCCTTTTGACGGTCG 5 0.125 No Hit ACCCAATGTTTATTGACCTATTCGAAAAGTTATTTGTGAAAACAGTTAAC 5 0.125 No Hit GATTCCACCACTGGATCAGGCTCCGCGAGTGGAGGGGGTAGGGGTGAAGG 5 0.125 No Hit GGTGAGTTTGAGTACGTCGATGACCACAGATCTGGCAAGATTGTTGTTGA 5 0.125 No Hit GTTTGCCCTTGTAAAAGAATTTTTTCGGAAGTTAATCCGGTATTGATATG 5 0.125 No Hit GCAGCACTCAAATTATTTAAGTTGTAATTTATCTTTCAGGCAGGTAACGT 5 0.125 No Hit GTTCTACTCACTGCCCTTTAATCTCAACAAGTTCTTCCCTTCGGTCTGAT 5 0.125 No Hit ATACAGCAGCAGCAGCAGGCGGTCTGCTTCTGAAGCTGCTGCAGGAGCTG 5 0.125 No Hit ATCAATTCCAGACGGCAGTGCTACTGCGACAAAGAAAAATGGAACTTGGG 5 0.125 No Hit TGAAAAGGCTGCTGATCTCCAGAACTATGTGCTGTGTGTTGAGATGCTCA 5 0.125 No Hit GTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAG 5 0.125 No Hit AGTGGTTCAAGTCAGTAAACTGGAAGAGACTCGAGGCTCGACAGATCCAG 5 0.125 No Hit GTTCGTTCGAGAACCAGCTTGTGCCTGGTTGGCGCATGTACAAAAGATTT 5 0.125 No Hit AGTCATGCACCTTTTGGGCATGATTTTAAATTTAACTTAACAACTGTTTG 5 0.125 No Hit CTCAGAAGCCGTCAATGAGGATCTGCAAGCATCGGGGGGACTCTTGGAGG 5 0.125 No Hit CGACTATGAGTGTCCAGCCCTTATCGGGAGTGTTCAGCCTTCAGCTAAGA 5 0.125 No Hit GCTTACTGCAAGATGAAGTGGTCTGTATCTCTGTTATCCATGTGGGAAAA 5 0.125 No Hit GTGATCCTGATGTGTGCAGAAACTGCTGGGTAGGGTGTGGTGATGGTTCA 5 0.125 No Hit AAGCGACGGCTGTGGTTGGAAACTATCCCTCTGGTTTGTATTTGTGCTAT 5 0.125 No Hit CCGACTACTAAGCAGATCGATGCAAGCTGCTTCTGCTTCCGAGTTCGTTC 5 0.125 No Hit CCTGGCCAACCCGGTGACCAACCACGTGCAGAGCGCGGAGCAGCACAACC 5 0.125 No Hit GTTGTGCAATTTGCAAACCAACAATTCAGACCAACACATAGCTTTGTCTC 5 0.125 No Hit TAAAAAGGTTTTTTGTCGGATGGCGTGCCGGGGTTTTCAGTGGGGTGCCT 5 0.125 No Hit GTTGCAGTTTGATACTTGATCTGAGTACCTCTAGTTATCCACCTATCTTG 5 0.125 No Hit CAGACCAGTTTCATCTACTTGCTATACATATGGATATAGCGGTGTCTGAT 5 0.125 No Hit AGACTGCAAGCTCAGCATTTCTGTAGGGCAATTTGCATTTTGTATTGTAG 5 0.125 No Hit ATCGACGCCGCCTGGGAGGCCGTGCGCCACGCCCGCAAGCCCCGCATCCA 5 0.125 No Hit GGCTCGTCTTGTTTTTTGGTAAATAATAATAAGCAAGGGGAGTGTATTTT 5 0.125 No Hit GTTGGGAAGCCATAAAACTGGAAGGAAGTGGGAATTATATTTTCGCTGAA 5 0.125 No Hit CCTCGATTTCACTGAAAACAGCGAGCAGGGCGACAGGTACCGAGAATTGG 5 0.125 No Hit CTTGTGTCAAACTAGCTAGAGGGACATCGAGCTGATCGAGCTCGAGGAAG 5 0.125 No Hit CAGTAATGAGGTTCTTTCCATTAATTTGCGCCCTTAATTAGCTGCTGTGA 5 0.125 No Hit CATGAAGATAGCTCAAGAGGAGATATTTGGGCCTGTTCAGTCAATCTTCA 5 0.125 No Hit GAGAGACGGCAGGACCAACATCTCCATTTTTGCAAGGTCTGAGGTTCGGC 5 0.125 No Hit TGGATGCTTGCGCATCACGGTAGCGAATAATGATTCTGGTGTTCGTGAAT 5 0.125 No Hit AGTATGACCTGTACAGCAAGAGCAGCGTCAGGATCGACGTCGAGAAAGTG 5 0.125 No Hit CTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGATCTC 5 0.125 No Hit GGCAGAGATGGCGCTTTAGGGATCTCTCAAAGCTGCGGTTGGGAGAGGAT 5 0.125 No Hit GTTGTACTTGCAAATGCAGGTTGCTGTTATTGTCCTACGGATTATATTTT 5 0.125 No Hit AGTCGACTGAGACAAAGAACCTTGCAGACTGTCATTTTTCAGATGGATCC 5 0.125 No Hit CACCGCTTTCTGAGCATCCCGAAAGTCGCGACCTCTCGGCGGATTTGGGA 5 0.125 No Hit GTTGTCACTAAATACTTGCTTAAGAGCGGCCTTCAAGAATACTTCAACAA 5 0.125 No Hit GTGAGAACTTGGAAAATCCAGAGCTCTACCACTATGCACTTTTCTCTGAC 5 0.125 No Hit GCATGCTGAGGATACTGCAACAGTTACTGCTGTGAAGTTTTGTACTGAAC 5 0.125 No Hit AGGGATTTCTCTCCCTCCCCCCCGCTCTCCCGCACGCATCAGCCTCAAGA 5 0.125 No Hit GGGCGGTGAATCTATCTATGGCACAAAGTTTGCTGATGAAAACTTCAAGC 5 0.125 No Hit AGAAAATTATCTGTATGCATGGTGGCATTGGTCGGTCAATCAACCATGTG 5 0.125 No Hit ATCTCTTCTTCCTCCTCGCGCGAGCGCCTCCATCCACCGCAGCGCAGGGA 5 0.125 No Hit TATATATGGCCCTCGGGCGACCATCGATCTGTGCATGATGGCGATCATGC 5 0.125 No Hit GAAATTAGCTTGGTCAAAATGGGGTGGCCACTTGAAAGGTCTTCCTATCT 5 0.125 No Hit ACAGTCCCTCTCCGCCATTGGTGGTGTTGAAACTGGCAATGATGCTGCTG 5 0.125 No Hit CCGAGAGTAATCCGCGAGTATGCGTGTATTTGTGTCGTGAGCGACTCATC 5 0.125 No Hit AGATTAGACAGGAACTTGATATGATGAGACGGGAAGTCAGCAAGAGGCGC 5 0.125 No Hit CAGAAATCCTGGGGGTTTTGTAGTAGAGTTAAAGACATCGAGTTTCTGTA 5 0.125 No Hit CATTGATTACCGTCACCTTGTTAGCACAGAATCAACATCTGGTGTTACAT 5 0.125 No Hit GCCAAGCTGAGCACGAAGGGAGGAGAGGCGGCTGTACTGTAGTCTGTAGA 5 0.125 No Hit GAGCAACCACCGCTAGATCGATCCCAGCCGTCAGACCGTAGAAGAATAGT 5 0.125 No Hit CTCACAGGCCGCAGCTAGCAAAGGTGCTTATTCACAGGTTGGATACTCAT 5 0.125 No Hit ACTTCCCTCCTAATTTGAAAATATGGAAGTCTAATGAGAAGCAGGATGAC 5 0.125 No Hit CCTCTCAGCTCTCCTCTCTCTCTTTCTCCGCCGCCGCAGTCTCCGAGGAA 5 0.125 No Hit CTGGAACCTTGCCAGGAAGAAGAAGCAGATTGAAGGCGAGGAAGCCTCCC 5 0.125 No Hit CAGTGGCTGGACAACAACCAGCTGGCTGAGGTGGACGAGTTCGAAGACAA 5 0.125 No Hit CATGGATCTCTATCTATCGAAATTTCAAATGGCCATCCCAATATGCGTAT 5 0.125 No Hit CTCCAATCGCCTCGAAGCTCGTCGAATCCTCCCCCACCTCCTCAAGATGC 5 0.125 No Hit GCCTCCTCCGGCTCGGTTTGGTCGGGAAGAAAGAACCATCGATTCGATCG 5 0.125 No Hit CGTGGCTTTTACCAGAGCATACGGCCCACACAGATGGGACTTTCACTGAA 5 0.125 No Hit TCTCTCTCCGCCTCCACTCTCTGATTCTGGGGATTTAATCTGGCCTCTTT 5 0.125 No Hit ATCGATTCCGCCTCGGCCGCCGACCGCGGCATACACTGCCCCCCGTCTCG 5 0.125 No Hit GTGGAGAAGTTCAATAGTGAAAAACACATAGAGTCCTCTCATTTAGGTGT 5 0.125 No Hit GTTGTGTTCCATGCAATTCAAAGGATGTACATTCCATGTCAGAGGATTCT 5 0.125 No Hit ATTTTTCTTACCGACACTTTTACTAAGAAGGAAGAGGATCCAATTTCTAG 5 0.125 No Hit AGCCATGGAAGAGCTAAAATGGGGGGATGACAACCACAAGTGGATCCGAC 5 0.125 No Hit GTTGAAGGAAATAAGAAGTTTCTGGCGATTGATAAAGCAATAGGTGAAAA 5 0.125 No Hit ATTCTCCTCCCACAACTCATCCAGAGAGCCAAGTGCTGGTACTGTTGTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052899 spots for ERR5262791.sra Written 1052899 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra Read 1052887 spots for ERR5262791.sra Written 1052887 spots for ERR5262791.sra SRR ids: ['ERR5262791.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_umo734x9 ERR5262791.sra spots: 21057752 blocks: [[1, 1052887], [1052888, 2105774], [2105775, 3158661], [3158662, 4211548], [4211549, 5264435], [5264436, 6317322], [6317323, 7370209], [7370210, 8423096], [8423097, 9475983], [9475984, 10528870], [10528871, 11581757], [11581758, 12634644], [12634645, 13687531], [13687532, 14740418], [14740419, 15793305], [15793306, 16846192], [16846193, 17899079], [17899080, 18951966], [18951967, 20004853], [20004854, 21057752]] ERR5262791 file size 6816620 ERR5262791 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262791 ERR5262791_1.fastq ERR5262791_2.fastq Input file: ERR5262791_1.fastq Paired file: ERR5262791_2.fastq trimmed: ERR5262791-trimmed-pair1.fastq, ERR5262791-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:40:10 2024 >> started Fri Dec 6 11:40:45 2024 >> done (34.606s) 21057752 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 21057752 (100.00%) read pairs available; of these: 6684 ( 0.03%) trimmed read pairs available after processing 21051068 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 1 0.00% 25 0 0.00% 26 0 0.00% 27 1 0.00% 28 1 0.00% 29 3 0.00% 30 3 0.00% 31 1 0.00% 32 2 0.00% 33 2 0.00% 34 2 0.00% 35 0 0.00% 36 3 0.00% 37 0 0.00% 38 0 0.00% 39 4 0.00% 40 0 0.00% 41 5 0.00% 42 0 0.00% 43 1 0.00% 44 1 0.00% 45 3 0.00% 46 3 0.00% 47 2 0.00% 48 2 0.00% 49 391 0.00% 50 487 0.00% 51 519 0.00% 52 552 0.00% 53 596 0.00% 54 639 0.00% 55 728 0.00% 56 835 0.00% 57 902 0.00% 58 1090 0.01% 59 1232 0.01% 60 1373 0.01% 61 1712 0.01% 62 1903 0.01% 63 2075 0.01% 64 2330 0.01% 65 2565 0.01% 66 2636 0.01% 67 3165 0.02% 68 3384 0.02% 69 4011 0.02% 70 4753 0.02% 71 5168 0.02% 72 6069 0.03% 73 6995 0.03% 74 7668 0.04% 75 8307 0.04% 76 9134 0.04% 77 9980 0.05% 78 10779 0.05% 79 12299 0.06% 80 13192 0.06% 81 15133 0.07% 82 16706 0.08% 83 18655 0.09% 84 20311 0.10% 85 22032 0.10% 86 22774 0.11% 87 24309 0.12% 88 26261 0.12% 89 26756 0.13% 90 29336 0.14% 91 31257 0.15% 92 32926 0.16% 93 36276 0.17% 94 38755 0.18% 95 39139 0.19% 96 41654 0.20% 97 43047 0.20% 98 43315 0.21% 99 45326 0.22% 100 47360 0.22% 101 48707 0.23% 102 51225 0.24% 103 53337 0.25% 104 54325 0.26% 105 57017 0.27% 106 58066 0.28% 107 58387 0.28% 108 59455 0.28% 109 61190 0.29% 110 62253 0.30% 111 63798 0.30% 112 66618 0.32% 113 66026 0.31% 114 69510 0.33% 115 70385 0.33% 116 71290 0.34% 117 73018 0.35% 118 74400 0.35% 119 72962 0.35% 120 73559 0.35% 121 74691 0.35% 122 75664 0.36% 123 76809 0.36% 124 80200 0.38% 125 79853 0.38% 126 80777 0.38% 127 81576 0.39% 128 82258 0.39% 129 82402 0.39% 130 83932 0.40% 131 82517 0.39% 132 83478 0.40% 133 84969 0.40% 134 85718 0.41% 135 86329 0.41% 136 87550 0.42% 137 86669 0.41% 138 88493 0.42% 139 89777 0.43% 140 87595 0.42% 141 90337 0.43% 142 92235 0.44% 143 90735 0.43% 144 90535 0.43% 145 92766 0.44% 146 93186 0.44% 147 172367 0.82% 148 89860 0.43% 149 89695 0.43% 150 16480413 78.26% 21057752 reads passed initial QC criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=37 prefix-density=0.58 prefix-fanout=2.0 sequence=TCGTCCTCATCG criterion=fanout-score sequence-density=0.04 sequence-density-rank=25 fanout-score=128.52 fanout-score-rank=1 prefix-density=0.80 prefix-fanout=6.7 sequence=GCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAG criterion=sequence-density sequence-density=3.22 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=29 prefix-density=3.22 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=29 fanout-score=125.88 fanout-score-rank=1 prefix-density=0.74 prefix-fanout=4.4 sequence=GATCAAGGAGGTTTCACACGAGTGGTCCTTGATTAACAAGCAGAAGCCTATCTGGATGAGGAAGCCTGAGGAAATTACCAAGGAGGAATATGCTGCTTTTTACAAGAGCCTGACCAACGACTGGGAGGAGCATCTTGCTGTCAAGCACTTCTCTGTGGAGGGGCAGCTTGAGTTCAAGGCGGTCCTCTTTGTCCCCAAGAGGGCCCCCTTTGACCTCTTTGACACCAAGAAGAAGGCCAACAACATCAAGCTCTATGTGCGCCGTGTCTTCATCATGGACAACTGTGAGGAGCTGATCCCAGAGTGGCTGGCCTTTGTCAAGGGTATTGTTGACTCTGAGGACCTTCCCCTCAACATCTCACGTGAGACCCTTCAGCAGAACAAGATCCTCAAGGTCATCCGCAAGAACCTTGTCAAGAAGTCCATCGAGCTCTTCTTTGAGATTGCTGAGAACAAGGAGGACTACAACAAGTTCTATGAGTCCTTCTCCAAGAACCTTAAACTTGGTATC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TCGTCCTCATCG -y CGGTTCCGGTTC -o ERR5262791 ERR5262791_1.fastq ERR5262791_2.fastq Input file: ERR5262791_1.fastq Paired file: ERR5262791_2.fastq trimmed: ERR5262791-trimmed-pair1.fastq, ERR5262791-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TCGTCCTCATCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:42:29 2024 >> started Fri Dec 6 11:42:36 2024 >> done (7.412s) 7019251 read pairs processed; of these: 27 ( 0.00%) short read pairs filtered out after trimming by size control 119 ( 0.00%) empty read pairs filtered out after trimming by size control 7019105 (100.00%) read pairs available; of these: 73 ( 0.00%) trimmed read pairs available after processing 7019032 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 1 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 1 0.00% 29 0 0.00% 30 2 0.00% 31 1 0.00% 32 1 0.00% 33 0 0.00% 34 0 0.00% 35 0 0.00% 36 0 0.00% 37 0 0.00% 38 0 0.00% 39 0 0.00% 40 0 0.00% 41 1 0.00% 42 0 0.00% 43 0 0.00% 44 0 0.00% 45 1 0.00% 46 2 0.00% 47 1 0.00% 48 0 0.00% 49 24 0.00% 50 33 0.00% 51 20 0.00% 52 44 0.00% 53 31 0.00% 54 44 0.00% 55 41 0.00% 56 306 0.00% 57 648 0.01% 58 671 0.01% 59 104 0.00% 60 44 0.00% 61 919 0.01% 62 759 0.01% 63 85 0.00% 64 1233 0.02% 65 1570 0.02% 66 121 0.00% 67 1679 0.02% 68 726 0.01% 69 2077 0.03% 70 1673 0.02% 71 1651 0.02% 72 717 0.01% 73 2997 0.04% 74 2727 0.04% 75 1923 0.03% 76 4243 0.06% 77 2715 0.04% 78 3678 0.05% 79 3800 0.05% 80 5182 0.07% 81 4361 0.06% 82 5277 0.08% 83 7496 0.11% 84 5714 0.08% 85 8293 0.12% 86 6433 0.09% 87 9027 0.13% 88 7631 0.11% 89 9891 0.14% 90 8634 0.12% 91 11105 0.16% 92 11170 0.16% 93 11183 0.16% 94 12741 0.18% 95 13408 0.19% 96 13781 0.20% 97 14382 0.20% 98 14581 0.21% 99 14925 0.21% 100 14978 0.21% 101 16536 0.24% 102 16749 0.24% 103 18855 0.27% 104 18134 0.26% 105 19744 0.28% 106 18793 0.27% 107 20098 0.29% 108 19864 0.28% 109 20194 0.29% 110 21536 0.31% 111 21428 0.31% 112 22312 0.32% 113 22996 0.33% 114 23071 0.33% 115 23595 0.34% 116 24830 0.35% 117 24128 0.34% 118 25866 0.37% 119 23729 0.34% 120 23653 0.34% 121 25460 0.36% 122 25296 0.36% 123 25829 0.37% 124 26987 0.38% 125 27248 0.39% 126 27134 0.39% 127 27056 0.39% 128 27538 0.39% 129 27890 0.40% 130 28541 0.41% 131 27822 0.40% 132 27732 0.40% 133 28260 0.40% 134 28813 0.41% 135 28416 0.40% 136 29057 0.41% 137 28882 0.41% 138 29252 0.42% 139 29699 0.42% 140 28695 0.41% 141 30400 0.43% 142 30504 0.43% 143 30339 0.43% 144 30195 0.43% 145 30294 0.43% 146 31278 0.45% 147 57909 0.83% 148 30668 0.44% 149 30214 0.43% 150 5488078 78.19% criterion=sequence-density sequence-density=0.16 sequence-density-rank=1 fanout-score=4.34 fanout-score-rank=28 prefix-density=0.25 prefix-fanout=2.9 sequence=AAGATCTGCATGCCACCACG criterion=fanout-score sequence-density=0.08 sequence-density-rank=22 fanout-score=244.63 fanout-score-rank=1 prefix-density=0.76 prefix-fanout=27.3 sequence=CTTCTTCTTCCT criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=2.22 fanout-score-rank=34 prefix-density=0.59 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.04 sequence-density-rank=30 fanout-score=511.24 fanout-score-rank=1 prefix-density=1.01 prefix-fanout=21.4 sequence=CCGCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGC ERR5262791 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:43:24 Started mapping on | Dec 06 11:43:25 Finished on | Dec 06 11:45:02 Mapping speed, Million of reads per hour | 781.52 Number of input reads | 21057606 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 20515846 Uniquely mapped reads % | 97.43% Average mapped length | 286.98 Number of splices: Total | 19150402 Number of splices: Annotated (sjdb) | 17690901 Number of splices: GT/AG | 18861085 Number of splices: GC/AG | 252382 Number of splices: AT/AC | 12251 Number of splices: Non-canonical | 24684 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.00% Deletion average length | 1.51 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 205169 % of reads mapped to multiple loci | 0.97% Number of reads mapped to too many loci | 684 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.58% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 336591 336591 336591 N_multimapping 205169 205169 205169 N_noFeature 1013455 19909507 1211360 N_ambiguous 472923 2815 64405 UnstrandedReadsAssigned:19029468 PositiveStrandReadsAssigned:603524 NegativeStrandReadsAssigned:19240081 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=145 echo kmer=141 ERR5262791 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262791-trimmed-pair1.fastq ERR5262791-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,057,606 reads, 19,462,241 reads pseudoaligned [quant] estimated average fragment length: 245.271 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,160 rounds 52973 ERR5262791.ke.tsv 35125 ERR5262791.se.tsv 88098 total ==> ERR5262791.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 692.188 0 0 PNS24247 1044 799.729 160.754 15.4417 PNS24249 1928 1683.73 266.448 12.1567 PNS24246 1044 799.729 160.754 15.4417 PNS24248 1044 799.729 160.754 15.4417 PNS24244 1471 1226.73 154.29 9.66198 PNS24243 293 115.196 0 0 KQK14069 1603 1358.73 41399.4 2340.66 KQK14071 474 254.487 671.381 202.666 ==> ERR5262791.se.tsv <== BRADI_1g14170v3 44303 BRADI_1g53295v3 218 BRADI_1g59795v3 845 BRADI_1g07683v3 0 BRADI_1g00485v3 31 BRADI_1g20270v3 473 BRADI_1g74790v3 1230 BRADI_1g09890v3 0 BRADI_1g77505v3 255 BRADI_1g48960v3 0 ERR5262791 completed mapping pipeline successfully