Starting /dee2/code/volunteer_pipeline.sh ERR5262792 current disk space = 1551311335424 free memory = 1595804608 ERR5262792 SRAfilesize 3cf22361614293f7f290ad7ac3584c36 ERR5262792.sra ERR5262792.sra file validated ERR5262792 is paired end ERR5262792 is conventional basespace ERR5262792 read1 length is 59-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262792_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 59-150 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.647 37.0 37.0 37.0 37.0 37.0 2 36.59325 37.0 37.0 37.0 37.0 37.0 3 36.615 37.0 37.0 37.0 37.0 37.0 4 36.619 37.0 37.0 37.0 37.0 37.0 5 36.595 37.0 37.0 37.0 37.0 37.0 6 36.65 37.0 37.0 37.0 37.0 37.0 7 36.519 37.0 37.0 37.0 37.0 37.0 8 36.5035 37.0 37.0 37.0 37.0 37.0 9 36.5805 37.0 37.0 37.0 37.0 37.0 10-14 36.6576 37.0 37.0 37.0 37.0 37.0 15-19 36.628699999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.6057 37.0 37.0 37.0 37.0 37.0 25-29 36.5817 37.0 37.0 37.0 37.0 37.0 30-34 36.643100000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.5846 37.0 37.0 37.0 37.0 37.0 40-44 36.532199999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.5328 37.0 37.0 37.0 37.0 37.0 50-54 36.549400000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.5515 37.0 37.0 37.0 37.0 37.0 60-64 36.52656328164082 37.0 37.0 37.0 37.0 37.0 65-69 36.45079562548349 37.0 37.0 37.0 37.0 37.0 70-74 36.40137552352238 37.0 37.0 37.0 37.0 37.0 75-79 36.501477962317395 37.0 37.0 37.0 37.0 37.0 80-84 36.48640443048247 37.0 37.0 37.0 37.0 37.0 85-89 36.34053400677195 37.0 37.0 37.0 37.0 37.0 90-94 36.31965492140076 37.0 37.0 37.0 37.0 37.0 95-99 36.403742696034726 37.0 37.0 37.0 37.0 37.0 100-104 36.42709306136206 37.0 37.0 37.0 37.0 37.0 105-109 36.35278612252006 37.0 37.0 37.0 37.0 37.0 110-114 36.31049540222229 37.0 37.0 37.0 37.0 37.0 115-119 36.271345375371936 37.0 37.0 37.0 37.0 37.0 120-124 36.30089371256764 37.0 37.0 37.0 37.0 37.0 125-129 36.352947557581196 37.0 37.0 37.0 37.0 37.0 130-134 36.24226708755407 37.0 37.0 37.0 37.0 37.0 135-139 36.26050229360929 37.0 37.0 37.0 37.0 37.0 140-144 36.248336299601334 37.0 37.0 37.0 37.0 37.0 145-149 36.28401558093681 37.0 37.0 37.0 37.0 37.0 150 36.11137868753763 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 0.0 27 2.0 28 8.0 29 14.0 30 10.0 31 26.0 32 31.0 33 59.0 34 88.0 35 219.0 36 2958.0 37 583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.050000000000004 12.4 10.274999999999999 32.275 2 28.986232790988737 13.76720901126408 28.060075093867333 29.18648310387985 3 21.224999999999998 22.475 24.5 31.8 4 23.45 29.825000000000003 18.075 28.65 5 15.15 42.25 20.95 21.65 6 19.75 39.425 21.2 19.625 7 11.0 30.099999999999998 39.2 19.7 8 16.35 26.775 30.675 26.200000000000003 9 16.725 25.025 35.8 22.45 10-14 18.970000000000002 37.125 23.785 20.119999999999997 15-19 21.36 30.48 23.235 24.925 20-24 19.79 30.314999999999998 25.89 24.005000000000003 25-29 22.225 30.325000000000003 24.275 23.175 30-34 22.34 31.290000000000003 25.335 21.035 35-39 20.93 30.23 29.215000000000003 19.625 40-44 21.91 32.074999999999996 26.169999999999998 19.845 45-49 22.259999999999998 28.29 28.075 21.375 50-54 18.72 29.244999999999997 29.675 22.36 55-59 24.64 32.06 23.885 19.415 60-64 21.02551275637819 31.695847923961978 26.758379189594798 20.520260130065033 65-69 21.952073640502277 32.08764820651358 27.845314923207766 18.114963229776375 70-74 20.972507386448996 34.268115579147675 27.868195703340177 16.891181331063148 75-79 17.323978952643447 36.19143071911802 30.00751691305437 16.477073415184165 80-84 18.543811197589758 33.84383630429325 26.115992970123024 21.496359527993974 85-89 20.908769983357708 31.050481617832464 30.833627515255436 17.20712088355439 90-94 19.754532491342434 26.60419637400693 35.740476675493994 17.900794459156653 95-99 18.853050288540807 29.6681780708986 30.502885408079145 20.97588623248145 100-104 18.027919574955725 29.508282112720075 34.84217105948536 17.621627252838838 105-109 18.94864879158073 24.623195303823575 34.375165265217625 22.052990639378073 110-114 20.848530358292212 26.44818708431667 31.05556747479082 21.6477150826003 115-119 17.828614899744608 27.615062761506277 32.9891865456719 21.567135793077217 120-124 21.09108889133062 24.63465553235908 34.99615426876168 19.27810130754862 125-129 20.27132213944179 21.45001667963972 32.992327365728904 25.286333815189593 130-134 17.740845070422534 20.467605633802815 37.31267605633803 24.47887323943662 135-139 25.328136642402704 20.341606006763342 33.69633747922279 20.633919871611166 140-144 21.690618062857812 20.901974529734783 32.684893094987736 24.722514312419676 145-149 23.17821605195734 23.76214026097837 30.41768456175892 22.64195912530537 150 24.292594822396147 21.041541240216738 29.650812763395546 25.01505117399157 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 0.5 25 0.0 26 0.0 27 0.5 28 15.0 29 26.5 30 78.5 31 78.5 32 36.0 33 41.0 34 67.5 35 116.5 36 146.0 37 173.5 38 244.5 39 248.5 40 182.0 41 295.0 42 367.0 43 365.5 44 374.5 45 233.0 46 131.5 47 128.5 48 190.5 49 185.5 50 95.5 51 62.5 52 32.5 53 7.0 54 2.5 55 13.5 56 18.0 57 22.5 58 22.5 59 7.5 60 7.0 61 7.0 62 3.0 63 2.0 64 0.5 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 58-59 2.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.0 70-71 3.0 72-73 0.0 74-75 2.0 76-77 1.0 78-79 4.0 80-81 3.0 82-83 5.0 84-85 7.0 86-87 9.0 88-89 12.0 90-91 17.0 92-93 34.0 94-95 12.0 96-97 11.0 98-99 17.0 100-101 17.0 102-103 26.0 104-105 15.0 106-107 37.0 108-109 16.0 110-111 18.0 112-113 21.0 114-115 16.0 116-117 27.0 118-119 15.0 120-121 8.0 122-123 16.0 124-125 25.0 126-127 12.0 128-129 13.0 130-131 32.0 132-133 17.0 134-135 27.0 136-137 23.0 138-139 35.0 140-141 16.0 142-143 28.0 144-145 35.0 146-147 19.0 148-149 23.0 150-151 3322.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.625 #Duplication Level Percentage of deduplicated Percentage of total 1 44.30939226519337 10.025 2 19.005524861878452 8.6 3 10.386740331491714 7.049999999999999 4 4.751381215469613 4.3 5 3.9779005524861875 4.5 6 2.8729281767955803 3.9 7 1.8784530386740332 2.9749999999999996 8 1.7679558011049725 3.2 9 1.9889502762430937 4.05 >10 8.176795580110499 35.575 >50 0.7734806629834254 12.2 >100 0.11049723756906078 3.6249999999999996 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAAGATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCT 145 3.6249999999999996 No Hit GCTGAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGC 97 2.4250000000000003 No Hit GGGGAAGATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAGC 81 2.025 No Hit CCACATTCCAATAGCTGTCTCCAATGCAAGAGATTTTTGACCCTTTTCCC 80 2.0 No Hit TGATAATTTAAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCAATG 75 1.875 No Hit CTCCACATTCCAATAGCTGTCTCCAATGCAAGAGATTTTTGACCCTTTTC 53 1.325 No Hit GCGGTAATTACAAACAAAGTTGTAGTACAACAGGGGAAGATGAGATAGGG 51 1.275 No Hit GCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGAGA 51 1.275 No Hit CTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGG 46 1.15 No Hit GACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATT 44 1.0999999999999999 No Hit GGAAGATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTC 43 1.075 No Hit GGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACTGGTATTCGCT 42 1.05 No Hit GCCACACCAACAAAGCCAAGAGCTAGAGATGAAGAAGTGACTAGCTCGTG 38 0.95 No Hit GGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACTGGTATTCGC 37 0.9249999999999999 No Hit CCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGAGAA 36 0.8999999999999999 No Hit GGTAATTACAAACAAAGTTGTAGTACAACAGGGGAAGATGAGATAGGGCA 36 0.8999999999999999 No Hit CCTCCACATTCCAATAGCTGTCTCCAATGCAAGAGATTTTTGACCCTTTT 34 0.8500000000000001 No Hit CCTGCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCATT 33 0.8250000000000001 No Hit GGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGAT 32 0.8 No Hit GATAATTTAAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCAATGT 31 0.775 No Hit GCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATG 31 0.775 No Hit CTTGAATCCATGGCACACGGACAGGATTATGATGAGAATGAGTGCAACAA 27 0.675 No Hit CTCCTGCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCA 26 0.65 No Hit ACTAAATTCAACAACAAAGATGCTTCAAAATGTACACAAATAATATCTTC 26 0.65 No Hit GGACTGCTGAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCC 25 0.625 No Hit CATCAAACAGCAACCTCCACATTCCAATAGCTGTCTCCAATGCAAGAGAT 24 0.6 No Hit GTGCTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTCCATC 24 0.6 No Hit GGGAGGAACAAATGAAGAGGAAAAGCATATGTACTTTCAATTCTATACAG 22 0.5499999999999999 No Hit GGAGAAACAAAGGAAATGCATGATAATTTAAATAAAGCAACTCCTGCTCA 21 0.525 No Hit GTAATTACAAACAAAGTTGTAGTACAACAGGGGAAGATGAGATAGGGCAC 21 0.525 No Hit ACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTA 19 0.475 No Hit CTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGAGAATG 19 0.475 No Hit GGCACACGGACAGGATTATGATGAGAATGAGTGCAACAATTATGCCGAGA 19 0.475 No Hit CCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTAT 19 0.475 No Hit GCACTTGAATCCATGGCACACGGACAGGATTATGATGAGAATGAGTGCAA 19 0.475 No Hit GCAACCTCCACATTCCAATAGCTGTCTCCAATGCAAGAGATTTTTGACCC 19 0.475 No Hit CTGCTGAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATG 18 0.44999999999999996 No Hit CATCAATGTATCTAAACTGATCGAGCCATTGTTTAAATAGATAGATGAGA 18 0.44999999999999996 No Hit GGGTCATCTAATTCCCTTCAAACCATTGTCAATTGAGTTAACTGACTGTA 18 0.44999999999999996 No Hit GTCGGCTGTACGGTGGGCACATTGTATGATAAGTACCAGGCTGGCTGTGA 18 0.44999999999999996 No Hit AGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACTGGTATTCG 17 0.42500000000000004 No Hit GTCTTTTGATGCTTTCTCTTAATGATTCTCCATTCCATCTGTGAGCAAAA 17 0.42500000000000004 No Hit CTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTCCATCAAT 17 0.42500000000000004 No Hit CTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCATTGTTT 16 0.4 No Hit CCTCGGTGCTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATT 16 0.4 No Hit GGCACATTGTATGATAAGTACCAGGCTGGCTGTGAGCTCAAGGCCGGCGT 16 0.4 No Hit CACACGGACAGGATTATGATGAGAATGAGTGCAACAATTATGCCGAGAAC 16 0.4 No Hit GCTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTCCATCAA 16 0.4 No Hit CATCAATGCAACTGGATCTGATGGTGCAGACATATTGGGATCACTGTTAC 15 0.375 No Hit CTTCCATCAAACAGCAACCTCCACATTCCAATAGCTGTCTCCAATGCAAG 15 0.375 No Hit GCCCATCATCACAAATGCTTGATGCAACTTCTAACAACCTAAGGTGACCA 15 0.375 No Hit CTTCACTCGTTGTGTGATTATGAACTTCCCACACTCGCATCAGTTCGAAT 15 0.375 No Hit TCCACATTCCAATAGCTGTCTCCAATGCAAGAGATTTTTGACCCTTTTCC 15 0.375 No Hit GCATGATAATTTAAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCA 14 0.35000000000000003 No Hit CACCAACAAAGCCAAGAGCTAGAGATGAAGAAGTGACTAGCTCGTGGTGG 14 0.35000000000000003 No Hit CTAAATTCAACAACAAAGATGCTTCAAAATGTACACAAATAATATCTTCA 13 0.325 No Hit GTCACATCAGAAGACTCTCTTGCTAATTCTGGCAGGTCTTGGAGCTTGTC 13 0.325 No Hit CTGCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCATTG 13 0.325 No Hit GGACAGGATTATGATGAGAATGAGTGCAACAATTATGCCGAGAACAATCA 13 0.325 No Hit TCCTGCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCAT 12 0.3 No Hit CCCCTGTATAATATTGACCAAAGAGCATAACCATTGGTTTTGCACCGAAG 12 0.3 No Hit CAACAAAGCCAAGAGCTAGAGATGAAGAAGTGACTAGCTCGTGGTGGGAT 12 0.3 No Hit GATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACT 12 0.3 No Hit CTCATGTCTTTTGATGCTTTCTCTTAATGATTCTCCATTCCATCTGTGAG 12 0.3 No Hit GACTGCTGAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCA 12 0.3 No Hit AGGGGAAGATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAG 12 0.3 No Hit GTATAATATTGACCAAAGAGCATAACCATTGGTTTTGCACCGAAGTTGCT 12 0.3 No Hit CAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCATTGTTTAA 11 0.27499999999999997 No Hit CCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGAGAAT 11 0.27499999999999997 No Hit CTGTCATCTCTCACTAATAGATCCAAAATTCATCGGTCAGCACTTCTGCC 11 0.27499999999999997 No Hit ATTTAAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCAATGTATCT 11 0.27499999999999997 No Hit GGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACTGGTATTCG 11 0.27499999999999997 No Hit GTGCAGACATATTGGGATCACTGTTACCAGATGAGTTTCCCTGGGAAGAA 11 0.27499999999999997 No Hit ATCAAACAGCAACCTCCACATTCCAATAGCTGTCTCCAATGCAAGAGATT 10 0.25 No Hit GCTAATTCTGGCAGGTCTTGGAGCTTGTCATGTTCACCTATAATATGTGT 10 0.25 No Hit CTCGTTGTGTGATTATGAACTTCCCACACTCGCATCAGTTCGAATTCTGA 10 0.25 No Hit ATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACA 10 0.25 No Hit CTTCAAACCATTGTCAATTGAGTTAACTGACTGTATCTTGAGGCCCCTTG 10 0.25 No Hit GTGATTATGAACTTCCCACACTCGCATCAGTTCGAATTCTGAAGAAACAT 10 0.25 No Hit GGCAGAACAAGGGATGGGAATAGAGATAACACATAAGTAATATCCACGTG 10 0.25 No Hit TGAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCAC 10 0.25 No Hit CACACCAACAAAGCCAAGAGCTAGAGATGAAGAAGTGACTAGCTCGTGGT 10 0.25 No Hit GTGCAGCTTTCTTCATGTAGAATTCCATCAATGCAACTGGATCTGATGGT 9 0.22499999999999998 No Hit CTGTATAATATTGACCAAAGAGCATAACCATTGGTTTTGCACCGAAGTTG 9 0.22499999999999998 No Hit CGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTCCATCAATGCAACTGG 9 0.22499999999999998 No Hit CAGCACTTTAAAACAACGCAGCACTTTATATTTCTTCTGTGTATACTGTG 9 0.22499999999999998 No Hit AAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCAATGTATCTAAAC 9 0.22499999999999998 No Hit AGAAAAAGGAGAAACAAAGGAAATGCATGATAATTTAAATAAAGCAACTC 9 0.22499999999999998 No Hit GTAGAATTCCATCAATGCAACTGGATCTGATGGTGCAGACATATTGGGAT 9 0.22499999999999998 No Hit GCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCATTGTT 9 0.22499999999999998 No Hit CTGGATCTGATGGTGCAGACATATTGGGATCACTGTTACCAGATGAGTTT 9 0.22499999999999998 No Hit GAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACA 9 0.22499999999999998 No Hit GTCACTATTTGCTTCTCCGGAGGCAATGATTTATTCTGTTTAAATTCCAT 9 0.22499999999999998 No Hit CCTGTATAATATTGACCAAAGAGCATAACCATTGGTTTTGCACCGAAGTT 9 0.22499999999999998 No Hit GTCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATG 9 0.22499999999999998 No Hit GCAACTCCTGCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGA 9 0.22499999999999998 No Hit CCCTGCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCGAGCCAT 9 0.22499999999999998 No Hit CACAGAAAAAGGAGAAACAAAGGAAATGCATGATAATTTAAATAAAGCAA 9 0.22499999999999998 No Hit ACTCGTTGTGTGATTATGAACTTCCCACACTCGCATCAGTTCGAATTCTG 9 0.22499999999999998 No Hit CCCGTGTACAGCTTGCACTTTTATAGCTGAATAAAAGCCTTCAGGTTAAC 9 0.22499999999999998 No Hit GTAAACGTAAAACTGTGAAGCTATTCAATCTAGTATACAACAAACTATCC 8 0.2 No Hit CCATTGTTTAAATAGATAGATGAGACATAGAAATGAACCAAGGCATTGTC 8 0.2 No Hit CTCGGTGCTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTC 8 0.2 No Hit GCAGCTTTCTTCATGTAGAATTCCATCAATGCAACTGGATCTGATGGTGC 8 0.2 No Hit AATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACAC 8 0.2 No Hit CTTCAAAATGTACACAAATAATATCTTCACTCGTTGTGTGATTATGAACT 8 0.2 No Hit AGCCACACCAACAAAGCCAAGAGCTAGAGATGAAGAAGTGACTAGCTCGT 8 0.2 No Hit ATGAAAACTGAAGGTTGATCAGAGCCAAGCTCGAAAGCCAGATTGAAAAG 8 0.2 No Hit CTACTAAATTCAACAACAAAGATGCTTCAAAATGTACACAAATAATATCT 8 0.2 No Hit ACTGGATCTGATGGTGCAGACATATTGGGATCACTGTTACCAGATGAGTT 8 0.2 No Hit CTGTAAACGTAAAACTGTGAAGCTATTCAATCTAGTATACAACAAACTAT 8 0.2 No Hit GTGTGATTATGAACTTCCCACACTCGCATCAGTTCGAATTCTGAAGAAAC 8 0.2 No Hit CCATCAAACAGCAACCTCCACATTCCAATAGCTGTCTCCAATGCAAGAGA 8 0.2 No Hit CACAAATAATATCTTCACTCGTTGTGTGATTATGAACTTCCCACACTCGC 8 0.2 No Hit CACAGAAACAGGATCCTTCAAATTCTTCTCAAGTTTCCTGACAATCTAAC 8 0.2 No Hit GTACAACAGGGGAAGATGAGATAGGGCACATAAGGGCAGCTGCTATGGAA 8 0.2 No Hit GTCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGAGA 7 0.17500000000000002 No Hit CTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTCCATCAATGC 7 0.17500000000000002 No Hit CCACAACAAGGTTGGATGGGGACCATGAAAACTGAAGGTTGATCAGAGCC 7 0.17500000000000002 No Hit GGAGGAACAAATGAAGAGGAAAAGCATATGTACTTTCAATTCTATACAGC 7 0.17500000000000002 No Hit GGCTGCTGAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCA 7 0.17500000000000002 No Hit GTCAGCACTTCTGCCATATCGTTTCCAGGAACATTTTGGGTCATCTAATT 7 0.17500000000000002 No Hit TGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACTGGTATTCG 7 0.17500000000000002 No Hit ATAATATTGACCAAAGAGCATAACCATTGGTTTTGCACCGAAGTTGCTGT 7 0.17500000000000002 No Hit AAATAATATCTTCACTCGTTGTGTGATTATGAACTTCCCACACTCGCATC 7 0.17500000000000002 No Hit ATTACAAACAAAGTTGTAGTACAACAGGGGAAGATGAGATAGGGCACATA 7 0.17500000000000002 No Hit ACACGGACAGGATTATGATGAGAATGAGTGCAACAATTATGCCGAGAACA 7 0.17500000000000002 No Hit ATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACTG 7 0.17500000000000002 No Hit TTTTTTTTTTCACAGAAAAAGGAGAAACAAAGGAAATGCATGATAATTTA 7 0.17500000000000002 No Hit AGCAACTCCTGCTCAAAAGTACAAACACATCAATGTATCTAAACTGATCG 7 0.17500000000000002 No Hit CGTCACATCAGAAGACTCTCTTGCTAATTCTGGCAGGTCTTGGAGCTTGT 7 0.17500000000000002 No Hit TGTATATACAATGCGGTAATTACAAACAAAGTTGTAGTACAACAGGGGAA 7 0.17500000000000002 No Hit ATCTGATGGTGCAGACATATTGGGATCACTGTTACCAGATGAGTTTCCCT 7 0.17500000000000002 No Hit GACATATTGGGATCACTGTTACCAGATGAGTTTCCCTGGGAAGAACTACC 6 0.15 No Hit ATAATTTAAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCAATGTA 6 0.15 No Hit CAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATG 6 0.15 No Hit ATGATAATTTAAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCAAT 6 0.15 No Hit CCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGA 6 0.15 No Hit GCCTCGGTGCTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAAT 6 0.15 No Hit CTGGCTGTGAGCTCAAGGCCGGCGTCTTAGGTGGCGACGGTGTCAAGTTC 6 0.15 No Hit CGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGAG 6 0.15 No Hit CGACACAATAAATACTTACTGTTACCAGATGAGTTTCCCTGGGAAGAACT 6 0.15 No Hit GGGCTGCTGAATGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCC 6 0.15 No Hit GCTGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATTATGATG 6 0.15 No Hit CTTCTGTGTATACTGTGTGTGGAATCAACTCTGAAATTAAAGTCATGCCC 6 0.15 No Hit GGCCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGATT 6 0.15 No Hit ATCAATGCAACTGGATCTGATGGTGCAGACATATTGGGATCACTGTTACC 6 0.15 No Hit GTGGAATCAACTCTGAAATTAAAGTCATGCCCGTGTACAGCTTGCACTTT 6 0.15 No Hit GGCAGGTCTTGGAGCTTGTCATGTTCACCTATAATATGTGTTTGAGGCAG 6 0.15 No Hit CAGCAACCTCCACATTCCAATAGCTGTCTCCAATGCAAGAGATTTTTGAC 6 0.15 No Hit GTTACCAGATGAGTTTCCCTGGGAAGAACTACCCTCATATGTCCCATATA 6 0.15 No Hit CTCCATGGCACACGGACAGGATTATGATGAGAATGAGTGCAACAATTATG 6 0.15 No Hit CTTGCACTTGAATCCATGGCACACGGACAGGATTATGATGAGAATGAGTG 6 0.15 No Hit GATCTGATGGTGCAGACATATTGGGATCACTGTTACCAGATGAGTTTCCC 6 0.15 No Hit TTTAAAACAACGCAGCACTTTATATTTCTTCTGTGTATACTGTGTGTGGA 6 0.15 No Hit GAAGATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCT 6 0.15 No Hit TGTATATGTATATACAATGCGGTAATTACAAACAAAGTTGTAGTACAACA 6 0.15 No Hit TCCCTGTATAATATTGACCAAAGAGCATAACCATTGGTTTTGCACCGAAG 6 0.15 No Hit GCCGTCACTATTTGCTTCTCCGGAGGCAATGATTTATTCTGTTTAAATTC 6 0.15 No Hit CCCTGTATAATATTGACCAAAGAGCATAACCATTGGTTTTGCACCGAAGT 5 0.125 No Hit CACGGACAGGATTATGATGAGAATGAGTGCAACAATTATGCCGAGAACAA 5 0.125 No Hit ATTTTTTTTTTCACAGAAAAAGGAGAAACAAAGGAAATGCATGATAATTT 5 0.125 No Hit GCTGATATCTCAAAGCATGGGAGCCCATCATCACAAATGCTTGATGCAAC 5 0.125 No Hit CGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAGGAT 5 0.125 No Hit TAATTACAAACAAAGTTGTAGTACAACAGGGGAAGATGAGATAGGGCACA 5 0.125 No Hit TCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGGACAG 5 0.125 No Hit AAAATGTACACAAATAATATCTTCACTCGTTGTGTGATTATGAACTTCCC 5 0.125 No Hit GTCATCTCTCACTAATAGATCCAAAATTCATCGGTCAGCACTTCTGCCAT 5 0.125 No Hit CTCACTAATAGATCCAAAATTCATCGGTCAGCACTTCTGCCATATCGTTT 5 0.125 No Hit AATTTAAATAAAGCAACTCCTGCTCAAAAGTACAAACACATCAATGTATC 5 0.125 No Hit GTAAACCAAGCTGCCCCTGCCAATTCGTCCATTAATTCATCAATGATTGG 5 0.125 No Hit AGATAGGGCACATAAGGGCAGCTGCTATGGAATGATCAGCTCTACTGGTA 5 0.125 No Hit AGAAACAGGATCCTTCAAATTCTTCTCAAGTTTCCTGACAATCTAACATG 5 0.125 No Hit CACTTGAATCCATGGCACACGGACAGGATTATGATGAGAATGAGTGCAAC 5 0.125 No Hit AGTACAAACACATCAATGTATCTAAACTGATCGAGCCATTGTTTAAATAG 5 0.125 No Hit GCCTTCCATCAAACAGCAACCTCCACATTCCAATAGCTGTCTCCAATGCA 5 0.125 No Hit CGGTGCTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTCCA 5 0.125 No Hit GTACAGCTTGCACTTTTATAGCTGAATAAAAGCCTTCAGGTTAACTTGAT 5 0.125 No Hit CAACAGGGGAAGATGAGATAGGGCACATAAGGGCAGCTGCTATGGAATGA 5 0.125 No Hit ACACCAACAAAGCCAAGAGCTAGAGATGAAGAAGTGACTAGCTCGTGGTG 5 0.125 No Hit GGATCTGATGGTGCAGACATATTGGGATCACTGTTACCAGATGAGTTTCC 5 0.125 No Hit AGAACAAGGGATGGGAATAGAGATAACACATAAGTAATATCCACGTGTGA 5 0.125 No Hit AATGCAACTGGATCTGATGGTGCAGACATATTGGGATCACTGTTACCAGA 5 0.125 No Hit TCCTGTGCAGCTTTCTTCATGTAGAATTCCATCAATGCAACTGGATCTGA 5 0.125 No Hit TGTATCTCGACCAGGCCGCCCTTACTTGCACTTGAATCCATGGCACACGG 5 0.125 No Hit TACACAAATAATATCTTCACTCGTTGTGTGATTATGAACTTCCCACACTC 5 0.125 No Hit TTCATGTAGAATTCCATCAATGCAACTGGATCTGATGGTGCAGACATATT 5 0.125 No Hit GTATACAACAAACTATCCTACTAAATTCAACAACAAAGATGCTTCAAAAT 5 0.125 No Hit CCATCATCACAAATGCTTGATGCAACTTCTAACAACCTAAGGTGACCATC 5 0.125 No Hit GCTTTCTTCATGTAGAATTCCATCAATGCAACTGGATCTGATGGTGCAGA 5 0.125 No Hit TTTTTTTTCACAGAAAAAGGAGAAACAAAGGAAATGCATGATAATTTAAA 5 0.125 No Hit CCTGTGCAGCTTTCTTCATGTAGAATTCCATCAATGCAACTGGATCTGAT 5 0.125 No Hit GGTCAATGGCGTAAACATATGCAGGGAGCATTATCAGTTTGCAATTTGGT 5 0.125 No Hit GTAGTACAACAGGGGAAGATGAGATAGGGCACATAAGGGCAGCTGCTATG 5 0.125 No Hit TGCTCTCTTTCGTTCTTCCTGTGCAGCTTTCTTCATGTAGAATTCCATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTTGG 30 4.077327E-4 29.944628 140-144 TGGCTTG 30 4.077327E-4 29.944628 140-144 GCTTGGT 25 0.0061466224 28.746843 140-144 TCTCAAT 25 0.0061466224 28.746843 140-144 GGTTGTG 35 0.0012172979 24.841526 135-139 GAGCTTC 35 0.0012172979 24.841526 135-139 GTTGTGG 35 0.0012172979 24.841526 135-139 TTGTGGT 35 0.0014131533 24.21854 130-134 TTTGTGG 35 0.0014131533 24.21854 130-134 GAAAGCC 45 0.00677135 18.48889 120-124 GGAAAGC 45 0.00677135 18.48889 120-124 AGAAGGG 45 0.0068934164 18.432175 115-119 GAAGGGA 45 0.0068934164 18.432175 115-119 ATAGGGC 55 0.0040109283 14.74909 10-14 GATAGGG 55 0.0040109283 14.74909 10-14 TGTCAGA 65 0.005474163 14.061972 105-109 GTCAGAG 65 0.005474163 14.061972 105-109 ATGTTTG 65 0.0064711887 13.714285 100-104 GAGGAAG 70 0.006837655 13.593633 110-114 AGGAAGA 70 0.006837655 13.593633 110-114 >>END_MODULE ERR5262792 read2 length is 59-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262792_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 59-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1465 37.0 37.0 37.0 37.0 37.0 2 36.0115 37.0 37.0 37.0 37.0 37.0 3 36.1025 37.0 37.0 37.0 37.0 37.0 4 36.0505 37.0 37.0 37.0 37.0 37.0 5 36.156 37.0 37.0 37.0 37.0 37.0 6 36.089 37.0 37.0 37.0 37.0 37.0 7 36.198 37.0 37.0 37.0 37.0 37.0 8 36.2405 37.0 37.0 37.0 37.0 37.0 9 36.1 37.0 37.0 37.0 37.0 37.0 10-14 36.2315 37.0 37.0 37.0 37.0 37.0 15-19 36.2273 37.0 37.0 37.0 37.0 37.0 20-24 36.19269999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.2019 37.0 37.0 37.0 37.0 37.0 30-34 36.175599999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.1148 37.0 37.0 37.0 37.0 37.0 40-44 36.097500000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.1215 37.0 37.0 37.0 37.0 37.0 50-54 36.0073 37.0 37.0 37.0 37.0 37.0 55-59 35.9851 37.0 37.0 37.0 37.0 37.0 60-64 36.026413206603294 37.0 37.0 37.0 37.0 37.0 65-69 35.98294333556571 37.0 37.0 37.0 37.0 37.0 70-74 35.95537992304878 37.0 37.0 37.0 37.0 37.0 75-79 35.90471254613075 37.0 37.0 37.0 37.0 37.0 80-84 35.82566765297438 37.0 37.0 37.0 37.0 37.0 85-89 35.85661477351706 37.0 37.0 37.0 37.0 37.0 90-94 35.75825174207017 37.0 37.0 37.0 37.0 37.0 95-99 35.77019189431182 37.0 37.0 37.0 37.0 37.0 100-104 35.80920744107281 37.0 37.0 37.0 37.0 37.0 105-109 35.693510764839836 37.0 37.0 37.0 37.0 37.0 110-114 35.73757674893739 37.0 37.0 37.0 37.0 37.0 115-119 35.70948075753525 37.0 37.0 37.0 37.0 37.0 120-124 35.644899520219475 37.0 37.0 37.0 37.0 37.0 125-129 35.700055268693895 37.0 37.0 37.0 37.0 37.0 130-134 35.542311752883855 37.0 37.0 37.0 37.0 37.0 135-139 35.55694341593243 37.0 37.0 37.0 37.0 37.0 140-144 35.47250803895416 37.0 37.0 37.0 37.0 37.0 145-149 35.49598800220399 37.0 37.0 37.0 37.0 37.0 150 35.57116953762466 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 2.0 13 4.0 14 2.0 15 1.0 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 7.0 23 4.0 24 7.0 25 5.0 26 11.0 27 9.0 28 13.0 29 23.0 30 15.0 31 38.0 32 60.0 33 89.0 34 170.0 35 555.0 36 2705.0 37 274.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.699999999999996 22.05 13.15 23.1 2 28.749999999999996 25.25 28.775000000000002 17.224999999999998 3 22.5 28.65 31.125000000000004 17.724999999999998 4 22.225 34.825 23.400000000000002 19.55 5 23.625 33.275 20.549999999999997 22.55 6 21.5 37.974999999999994 24.025 16.5 7 21.2 20.349999999999998 39.4 19.05 8 21.05 24.325 26.625 28.000000000000004 9 22.35 24.55 31.3 21.8 10-14 23.585 27.87 26.355 22.189999999999998 15-19 24.015 29.645 24.89 21.45 20-24 23.805 28.68 26.69 20.825 25-29 23.39 28.975 26.36 21.275 30-34 22.675 30.555 26.640000000000004 20.13 35-39 23.275000000000002 29.78 25.805 21.14 40-44 22.78 29.659999999999997 26.125 21.435000000000002 45-49 24.065 29.635 25.255 21.044999999999998 50-54 22.85 30.705 26.840000000000003 19.605 55-59 23.294999999999998 30.599999999999998 25.66 20.445 60-64 23.251625812906454 29.299649824912454 27.22361180590295 20.225112556278138 65-69 22.852568912902097 29.696332983140728 26.959827905347943 20.491270198609236 70-74 22.269517752516403 29.450648505183036 27.80309479693525 20.476738945365316 75-79 23.157103482836384 28.30368328739664 27.456777749937363 21.082435479829616 80-84 22.164197840823498 29.324629676123525 27.155410494602062 21.355761988450915 85-89 22.562912905340664 28.47849109889556 28.06495536839982 20.893640627363965 90-94 23.605805958747137 28.841354723707664 27.542653425006364 20.010185892538836 95-99 23.156212956759266 28.088439931969283 28.907900840076277 19.847446271195178 100-104 23.388735476475798 28.750065127911217 28.244672536862396 19.616526858750586 105-109 21.597883597883598 28.513227513227513 27.78306878306878 22.105820105820108 110-114 23.660067600193145 28.74617737003058 27.732174472879446 19.86158055689683 115-119 21.9752146972497 31.36210457658441 26.057180128274815 20.605500597891073 120-124 21.921195801505743 29.41144144639226 28.669560916634612 19.997801835467385 125-129 22.60540660807654 29.463789075536766 27.672711091333852 20.258093225052843 130-134 22.76903996843114 29.91713174361576 27.13794464174982 20.17588364620328 135-139 22.132276954874268 29.371914111838326 27.959582041566193 20.536226891721206 140-144 23.074219436471207 28.563060160506122 28.31117099174038 20.05154941128229 145-149 22.772395646453774 29.781126659490493 27.036239684248297 20.41023800980744 150 23.239649440918704 26.745240253853126 30.61347839226352 19.401631912964643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 2.0 25 2.5 26 2.0 27 1.5 28 2.5 29 2.0 30 0.0 31 3.0 32 8.5 33 22.0 34 54.0 35 75.5 36 93.0 37 140.0 38 167.5 39 218.5 40 306.5 41 317.5 42 265.5 43 250.5 44 262.0 45 268.5 46 282.5 47 261.0 48 195.0 49 175.5 50 160.0 51 85.0 52 44.0 53 42.0 54 40.0 55 37.0 56 30.0 57 31.0 58 21.5 59 11.0 60 14.0 61 10.5 62 11.0 63 13.0 64 9.5 65 11.5 66 14.0 67 12.0 68 10.5 69 7.0 70 6.5 71 5.0 72 4.5 73 5.0 74 2.5 75 1.5 76 2.0 77 2.5 78 2.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 58-59 2.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.0 70-71 3.0 72-73 0.0 74-75 2.0 76-77 1.0 78-79 4.0 80-81 3.0 82-83 5.0 84-85 7.0 86-87 9.0 88-89 12.0 90-91 17.0 92-93 35.0 94-95 12.0 96-97 11.0 98-99 17.0 100-101 17.0 102-103 26.0 104-105 16.0 106-107 37.0 108-109 15.0 110-111 18.0 112-113 21.0 114-115 16.0 116-117 27.0 118-119 15.0 120-121 8.0 122-123 16.0 124-125 25.0 126-127 13.0 128-129 13.0 130-131 33.0 132-133 16.0 134-135 30.0 136-137 26.0 138-139 37.0 140-141 15.0 142-143 28.0 144-145 38.0 146-147 20.0 148-149 23.0 150-151 3309.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.224999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 53.080836630864894 23.474999999999998 2 21.424533634821934 18.95 3 10.344827586206897 13.725000000000001 4 5.822498586772188 10.299999999999999 5 3.165630299604296 7.000000000000001 6 1.7524024872809498 4.65 7 1.0175240248728095 3.15 8 0.791407574901074 2.8000000000000003 9 0.6218202374222724 2.475 >10 1.978518937252685 13.475000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTCCTTCCATCCCGTTTCTTCTTCCTAAGCTTGTCGGCCTCTTGATCGA 50 1.25 No Hit GTTTCTTCTTCCTAAGCTTGTCGGCCTCTTGATCGATCCCACCACGAGCT 39 0.975 No Hit GGGGAATGTTTGTCTGGGGCTGCTAAATGGAGCAGAGATTGATCATGCGT 27 0.675 No Hit GTTTCTCTGCGCGGCAAGTTAGTTGTGTATGACAATGAACGGAGGCAAAT 26 0.65 No Hit GCCACAACCACAAAAGGGCTTTCCCTTCTTCCTCTGACAAACATTTGCAT 21 0.525 No Hit GTGTGACTGATCTTACACATGCGCTTCTGCAAGGAGAGAATGAGAATCAA 19 0.475 No Hit AGAGAATCTACGCTCACAGGCTCAAGATTTCAGGCAGCAAGGAACGCAGG 18 0.44999999999999996 No Hit TGTTTCTCTGCGCGGCAAGTTAGTTGTGTATGACAATGAACGGAGGCAAA 17 0.42500000000000004 No Hit GCTGATTCGGAATGCACCAAGCCACAACCACAAAAGGGCTTTCCCTTCTT 15 0.375 No Hit AAAAGATTGAACTGCTTGTTGACAAGACAGAGAATCTACGCTCACAGGCT 15 0.375 No Hit GGGAGATGTTTCTCTGCGCGGCAAGTTAGTTGTGTATGACAATGAACGGA 15 0.375 No Hit GCAGTATTGTGTGGACCACCCAGAAGAGATAAATAAGCTTGCTAAAGTGC 15 0.375 No Hit GGTAGATTCAATTGAGAAGCTCCGTGAGAAACTACCATCATTGCGAGCTG 14 0.35000000000000003 No Hit ATTCAATTGAGAAGCTCCGTGAGAAACTACCATCATTGCGAGCTGAGATA 14 0.35000000000000003 No Hit CAAGATTTCAGGCAGCAAGGAACGCAGGTAAGGAGAAAGATGTGGCTACA 14 0.35000000000000003 No Hit CGTTTCTTCTTCCTAAGCTTGTCGGCCTCTTGATCGATCCCACCACGAGC 13 0.325 No Hit AGTTAGTTGTGTATGACAATGAACGGAGGCAAATTGGATGGGCTGATTCG 13 0.325 No Hit GAAAGATGTGGCTACAAAACATGAAGATCAAGCTGATTGTTCTCGGCATA 12 0.3 No Hit GCTTTATATGGGACATATGAGGGTAGTTCTTCCCAGGGAAACTCATCTGG 12 0.3 No Hit AAGACAGAGAATCTACGCTCACAGGCTCAAGATTTCAGGCAGCAAGGAAC 12 0.3 No Hit GGCAAATTGGATGGGCTGATTCGGAATGCACCAAGCCACAACCACAAAAG 12 0.3 No Hit GATGTTTCTCTGCGCGGCAAGTTAGTTGTGTATGACAATGAACGGAGGCA 12 0.3 No Hit GTGACTGATCTTACACATGCGCTTCTGCAAGGAGAGAATGAGAATCAAAT 11 0.27499999999999997 No Hit ATTTCAGGCAGCAAGGAACGCAGGTAAGGAGAAAGATGTGGCTACAAAAC 11 0.27499999999999997 No Hit CACCAAGCCACAACCACAAAAGGGCTTTCCCTTCTTCCTCTGACAAACAT 11 0.27499999999999997 No Hit GGAACGCAGGTAAGGAGAAAGATGTGGCTACAAAACATGAAGATCAAGCT 11 0.27499999999999997 No Hit GTTAGTTGTGTATGACAATGAACGGAGGCAAATTGGATGGGCTGATTCGG 10 0.25 No Hit CAGGCTTCAAAAAATGTTAATTCTAGTGTTGAAAGCTCCAGAGCACATAC 10 0.25 No Hit GCAAGGAGAGAATGAGAATCAAATGAGAGAGAAAACTGACAATGCCTTGG 10 0.25 No Hit GTCATGCTTGTCATATCATGGCACATGAAAGCTGCCACAATGTGTGAATT 10 0.25 No Hit GGAAAGCAGCTACTGCTGCAGCAAGCAGCCTCAACAGAGAGTTTGGATCA 10 0.25 No Hit CTACGCTCACAGGCTCAAGATTTCAGGCAGCAAGGAACGCAGGTAAGGAG 10 0.25 No Hit AGGAACGCAGGTAAGGAGAAAGATGTGGCTACAAAACATGAAGATCAAGC 10 0.25 No Hit TCTTGATCGTGGAGAAAAGATTGAACTGCTTGTTGACAAGACAGAGAATC 10 0.25 No Hit AAATAAGCTTGCTAAAGTGCAAGCACAAGTTTCAGAAGTCAAAAACGTTA 10 0.25 No Hit GAAGAGATAAATAAGCTTGCTAAAGTGCAAGCACAAGTTTCAGAAGTCAA 9 0.22499999999999998 No Hit GGAAAACATCGAGAAGGTTCTTGATCGTGGAGAAAAGATTGAACTGCTTG 9 0.22499999999999998 No Hit GGAAAAGGTTCTTGCTCAGACAAAGGAGCCTCAGGCTTCAAAAAATGTTA 9 0.22499999999999998 No Hit GGAGCCTCAGGCTTCAAAAAATGTTAATTCTAGTGTTGAAAGCTCCAGAG 9 0.22499999999999998 No Hit GATTGATCATGCGTCAACACTAATAGTGGGAGATGTTTCTCTGCGCGGCA 9 0.22499999999999998 No Hit GCAAGCACAAGTTTCAGAAGTCAAAAACGTTATGATGGAAAACATCGAGA 9 0.22499999999999998 No Hit GTTAATTCTAGTGTTGAAAGCTCCAGAGCACATACTGGTCCACAGAGAAG 9 0.22499999999999998 No Hit TGAACTGCTTGTTGACAAGACAGAGAATCTACGCTCACAGGCTCAAGATT 9 0.22499999999999998 No Hit GTTGTGTATGACAATGAACGGAGGCAAATTGGATGGGCTGATTCGGAATG 9 0.22499999999999998 No Hit TCTACGCTCACAGGCTCAAGATTTCAGGCAGCAAGGAACGCAGGTAAGGA 9 0.22499999999999998 No Hit CTGAAGCTGCTGCTCTGCCTGGCCTAAGGGCTGAACTGGAAGCACTGAAA 9 0.22499999999999998 No Hit GGCACATCTCAACTTTGCAACGACCTGTTGGTGGATCCTCAGGATGTTGT 8 0.2 No Hit AGATTTCACAGTTTTGTGCATAAAACGACTATCAACCTCACAGACTGGCA 8 0.2 No Hit GACTGATCTTACACATGCGCTTCTGCAAGGAGAGAATGAGAATCAAATGA 8 0.2 No Hit CAAACATTTGCATAATAATAAACATATGTATAATTGATACATAGCGAATA 8 0.2 No Hit CTTACACATGCGCTTCTGCAAGGAGAGAATGAGAATCAAATGAGAGAGAA 8 0.2 No Hit GGACCACCCAGAAGAGATAAATAAGCTTGCTAAAGTGCAAGCACAAGTTT 8 0.2 No Hit CTCCTGGCATATTTGCATTTGCATTCGTGCAGATCACGGTGCATCTGCTG 8 0.2 No Hit GACCTGTTGGTGGATCCTCAGGATGTTGTCATGCTTGTCATATCATGGCA 8 0.2 No Hit TATGGTGGTGGGAAAGCAGCTACTGCTGCAGCAAGCAGCCTCAACAGAGA 8 0.2 No Hit GTTTCAGAAGTCAAAAACGTTATGATGGAAAACATCGAGAAGGTTCTTGA 8 0.2 No Hit CAGAGAATCTACGCTCACAGGCTCAAGATTTCAGGCAGCAAGGAACGCAG 8 0.2 No Hit CTCGGATATGTGTGACTGATCTTACACATGCGCTTCTGCAAGGAGAGAAT 8 0.2 No Hit CACAAATCTAACTATCAAACACCTGCTTCAGCTTTATATGGGACATATGA 8 0.2 No Hit AAGAGCGTGATGCTGATATGATCATGGTGGAGGGCACATCTCAACTTTGC 8 0.2 No Hit AAAACATCGAGAAGGTTCTTGATCGTGGAGAAAAGATTGAACTGCTTGTT 7 0.17500000000000002 No Hit GGAATGTTTGTCTGGGGCTGCTAAATGGAGCAGAGATTGATCATGCGTCA 7 0.17500000000000002 No Hit GGGCACATCTCAACTTTGCAACGACCTGTTGGTGGATCCTCAGGATGTTG 7 0.17500000000000002 No Hit CACAAAAGGGCTTTCCCTTCTTCCTCTGACAAACATTTGCATAATAATAA 7 0.17500000000000002 No Hit GTGTGGACCACCCAGAAGAGATAAATAAGCTTGCTAAAGTGCAAGCACAA 7 0.17500000000000002 No Hit GTTGACAAGACAGAGAATCTACGCTCACAGGCTCAAGATTTCAGGCAGCA 7 0.17500000000000002 No Hit CCTAAATTAAGTTATAGGTCGTGGGTTTTCATAGCTCGGATATGTGTGAC 7 0.17500000000000002 No Hit GGCAGATCTACGTGGGCGCCCTACCCCACGGTCCTCCGTAACCATGCATA 7 0.17500000000000002 No Hit GGTAAGGAGAAAGATGTGGCTACAAAACATGAAGATCAAGCTGATTGTTC 7 0.17500000000000002 No Hit GCAGTATATACCTAAATTAAGTTATAGGTCGTGGGTTTTCATAGCTCGGA 7 0.17500000000000002 No Hit GATTGAACTGCTTGTTGACAAGACAGAGAATCTACGCTCACAGGCTCAAG 7 0.17500000000000002 No Hit AGCACATGCAGTATTGTGTGGACCACCCAGAAGAGATAAATAAGCTTGCT 7 0.17500000000000002 No Hit GCCTCAGGCTTCAAAAAATGTTAATTCTAGTGTTGAAAGCTCCAGAGCAC 7 0.17500000000000002 No Hit GTGATGAAGAGTTAGAAGAGTTACGAAATGATATTGTTGACCTAAAGGAT 7 0.17500000000000002 No Hit GAGATGTTTCTCTGCGCGGCAAGTTAGTTGTGTATGACAATGAACGGAGG 7 0.17500000000000002 No Hit GCTAAATGGAGCAGAGATTGATCATGCGTCAACACTAATAGTGGGAGATG 7 0.17500000000000002 No Hit GCCAGCTCCAGACATTGGGCGCTCGTGTATAGCAGTGAATTTGACATCCC 7 0.17500000000000002 No Hit GGGAGGATTACTGCATAACGCTGCTGCCTAGGAACTATGAGTTTTACTGG 7 0.17500000000000002 No Hit ATTTTATCAGGTCCCTTCTGGCTCGTGCCCACTAGGTTTTGCTTTTGGAA 6 0.15 No Hit GTTTTCATAGCTCGGATATGTGTGACTGATCTTACACATGCGCTTCTGCA 6 0.15 No Hit AAGGAACGCAGGTAAGGAGAAAGATGTGGCTACAAAACATGAAGATCAAG 6 0.15 No Hit CACTAATAGTGGGAGATGTTTCTCTGCGCGGCAAGTTAGTTGTGTATGAC 6 0.15 No Hit GCTTGCTAAAGTGCAAGCACAAGTTTCAGAAGTCAAAAACGTTATGATGG 6 0.15 No Hit CAAAAACGTTATGATGGAAAACATCGAGAAGGTTCTTGATCGTGGAGAAA 6 0.15 No Hit GAGAGAGAAAACTGACAATGCCTTGGTTCATTTCTATGTCTCATCTATCT 6 0.15 No Hit GCTCAAGATTTCAGGCAGCAAGGAACGCAGGTAAGGAGAAAGATGTGGCT 6 0.15 No Hit GGGAATGTTTGTCTGGGGCTGCTAAATGGAGCAGAGATTGATCATGCGTC 6 0.15 No Hit TGAGAAGCTCCGTGAGAAACTACCATCATTGCGAGCTGAGATAAAAGATG 6 0.15 No Hit TGTGAATTTACTCGCCAGGAATTCTTTGATGGGCTGCAGTCAATTGGGGT 6 0.15 No Hit CTGCAGTCAATTGGGGTAGATTCAATTGAGAAGCTCCGTGAGAAACTACC 6 0.15 No Hit GCTGATTGTTCTCGGCATAATTGTTGCACTCATTCTCATCATAATCCTGT 6 0.15 No Hit CTCACAGACTGGCATTAGTGTCTTTGAATTTGAACAGACATGCAACTCCG 6 0.15 No Hit GTTCAACTACCTCGTCGAGGACGGCTTCACATACTGTGTAGTTGCCGTTG 6 0.15 No Hit CTCCGGTCCATAAACTTGAGAATCCCACATGTAGCAGTATATACCTAAAT 6 0.15 No Hit CAAACACCTGCTTCAGCTTTATATGGGACATATGAGGGTAGTTCTTCCCA 6 0.15 No Hit GTTTGGATCAAAACTTAAAGAGCACATGCAGTATTGTGTGGACCACCCAG 6 0.15 No Hit GCCTGGCCTAAGGGCTGAACTGGAAGCACTGAAACAAAGACACTTTCAAG 6 0.15 No Hit GTGAGAAACTACCATCATTGCGAGCTGAGATAAAAGATGATCATAAGTTC 6 0.15 No Hit CCCACATGTAGCAGTATATACCTAAATTAAGTTATAGGTCGTGGGTTTTC 6 0.15 No Hit CATCGAGAAGGTTCTTGATCGTGGAGAAAAGATTGAACTGCTTGTTGACA 6 0.15 No Hit GTTTTTTTGCTACACTTATATTCCAGGATAAGGGGAATGTTTGTCTGGGG 6 0.15 No Hit GATGGTTTGTCATTCCCAGAACATTCACCATTCTTCCCGATGATTACTTG 6 0.15 No Hit ACGCTCACAGGCTCAAGATTTCAGGCAGCAAGGAACGCAGGTAAGGAGAA 6 0.15 No Hit CCTGGAGATACACCATTCAAGTTTCTATTTGAAAAGAACTGTCCAGACTA 6 0.15 No Hit GATACACCATTCAAGTTTCTATTTGAAAAGAACTGTCCAGACTACAAATA 6 0.15 No Hit GGAACTATGAGTTTTACTGGAGTTTGCTAATATTTTCTAATTACCTAGAT 6 0.15 No Hit AGAGAACAAGTGGATCTTCTTGTTGGCCAGCTCCAGACATTGGGCGCTCG 6 0.15 No Hit AAATTGGTTCAGCTCAAAATCAGCATAGAAGATGCAAACCCATCTGACTT 6 0.15 No Hit AAACATTTGCATAATAATAAACATATGTATAATTGATACATAGCGAATAC 6 0.15 No Hit TGATCATCAGTGATAAGGGGAATGTTTGTCTGGGGCTGCTAAATGGAGCA 5 0.125 No Hit ACGCAGGTAAGGAGAAAGATGTGGCTACAAAACATGAAGATCAAGCTGAT 5 0.125 No Hit TTGAACACAAATCTAACTATCAAACACCTGCTTCAGCTTTATATGGGACA 5 0.125 No Hit ATTGAACTGCTTGTTGACAAGACAGAGAATCTACGCTCACAGGCTCAAGA 5 0.125 No Hit GACTCGGCTTGCTGAAGAGGAAAAGGTTCTTGCTCAGACAAAGGAGCCTC 5 0.125 No Hit ATGGATGCTAATCTGTAGCAAGAGGGAGAGGCAAAAACTCATGTTACTAT 5 0.125 No Hit CTTTGATGGGCTGCAGTCAATTGGGGTAGATTCAATTGAGAAGCTCCGTG 5 0.125 No Hit ACCACCCAGAAGAGATAAATAAGCTTGCTAAAGTGCAAGCACAAGTTTCA 5 0.125 No Hit TGATGCTGATATGATCATGGTGGAGGGCACATCTCAACTTTGCAACGACC 5 0.125 No Hit CAGTAGGGCAACAAATGCCCATTGCTTTCTTGGTTAGGATAAAGGATGAT 5 0.125 No Hit GGTTTACAGGGTGTCGCCATGTCTCTGAACTTGACACCGTCGCCACCTAA 5 0.125 No Hit TGAAGTAGAAAAAGGAGCTGCAAGCTTGCTAGCGCTGGACTTGTCCTCCC 5 0.125 No Hit AAAATGTTAATTCTAGTGTTGAAAGCTCCAGAGCACATACTGGTCCACAG 5 0.125 No Hit CGGACGGACAAGGGAAGCCATGGGGCAGCAGTCGCTGATCTACGCGTTCG 5 0.125 No Hit CGCTCACAGGCTCAAGATTTCAGGCAGCAAGGAACGCAGGTAAGGAGAAA 5 0.125 No Hit CTGATTCGGAATGCACCAAGCCACAACCACAAAAGGGCTTTCCCTTCTTC 5 0.125 No Hit GGAGAGAATGAGAATCAAATGAGAGAGAAAACTGACAATGCCTTGGTTCA 5 0.125 No Hit GAACAGACATGCAACTCCGGTCCATAAACTTGAGAATCCCACATGTAGCA 5 0.125 No Hit TTTCAGGCAGCAAGGAACGCAGGTAAGGAGAAAGATGTGGCTACAAAACA 5 0.125 No Hit GTGCAAGCACAAGTTTCAGAAGTCAAAAACGTTATGATGGAAAACATCGA 5 0.125 No Hit TTCCACTGCCCTGAGAAGTATGAAAATGAAGAACGCCTTGCCAATCACAA 5 0.125 No Hit ACGGAGGCAAATTGGATGGGCTGATTCGGAATGCACCAAGCCACAACCAC 5 0.125 No Hit CAGATAACTTATACCACACAGAAGCCCAGAAAGTAAATTATGGAGATCAG 5 0.125 No Hit GTCAGCAGAAAATAATAATGTCATACTATCTGAGGAGCATGACTCCAAGT 5 0.125 No Hit GATTCGGAATGCACCAAGCCACAACCACAAAAGGGCTTTCCCTTCTTCCT 5 0.125 No Hit TTCTCTGCGCGGCAAGTTAGTTGTGTATGACAATGAACGGAGGCAAATTG 5 0.125 No Hit ATAAACTTGAGAATCCCACATGTAGCAGTATATACCTAAATTAAGTTATA 5 0.125 No Hit GGGACATATGAGGGTAGTTCTTCCCAGGGAAACTCATCTGGTAACAGTGA 5 0.125 No Hit CATATCATGGCACATGAAAGCTGCCACAATGTGTGAATTTACTCGCCAGG 5 0.125 No Hit GAGAAGGTTGCCCTTCAGGCACTGAAAGCTAGCGATTGGCACTTGGAAGG 5 0.125 No Hit GTGAATTTGACATCCCATTTAATTTTTAATGCACTGCATCGACCGAGACC 5 0.125 No Hit GGGAAATTTGATCAGAGGAACTTTTACTATGACCGGGTTTCGTGGGAGGA 5 0.125 No Hit GGATGTCACACGGCAGAGGAATCCTGGAGATACACCATTCAAGTTTCTAT 5 0.125 No Hit TCAATTGAGAAGCTCCGTGAGAAACTACCATCATTGCGAGCTGAGATAAA 5 0.125 No Hit AAGAAGACAAGGTCTCAAGCTATTACGTGCTCAAGGTGATTGCAGATGCC 5 0.125 No Hit GCAGGGGATTCACAAGAAAGGTTTTACAGATGATGAGTTGAAGGACCGTG 5 0.125 No Hit AAAACTGATTCAGAAATTTTGTTGCAATCTGAGACCAATGAAGTAGAAAA 5 0.125 No Hit CTTGAGAATCCCACATGTAGCAGTATATACCTAAATTAAGTTATAGGTCG 5 0.125 No Hit GGTGGATCCTCAGGATGTTGTCATGCTTGTCATATCATGGCACATGAAAG 5 0.125 No Hit GTCACACGGCAGAGGAATCCTGGAGATACACCATTCAAGTTTCTATTTGA 5 0.125 No Hit CAGTATTGTGTGGACCACCCAGAAGAGATAAATAAGCTTGCTAAAGTGCA 5 0.125 No Hit CAATTGAGAAGCTCCGTGAGAAACTACCATCATTGCGAGCTGAGATAAAA 5 0.125 No Hit CCCTAAGGCACCCTCCTCCCTCCTCCTTCCGTCTGGCATCTCCCCTCCCA 5 0.125 No Hit CACAGTTTTGTGCATAAAACGACTATCAACCTCACAGACTGGCATTAGTG 5 0.125 No Hit GTGAATTTGGTGATGAACAAGAACAAGGTACAAGCATTTTGTATCTGCTG 5 0.125 No Hit GAGAAGGTTCTTGATCGTGGAGAAAAGATTGAACTGCTTGTTGACAAGAC 5 0.125 No Hit GTCCAGATAACTTATACCACACAGAAGCCCAGAAAGTAAATTATGGAGAT 5 0.125 No Hit GGGCTACTGAGATGGGTCAAGCGTTCATCTCGACGACCTTCTTCTGGATT 5 0.125 No Hit GGGTTTTCATAGCTCGGATATGTGTGACTGATCTTACACATGCGCTTCTG 5 0.125 No Hit AGAAGCTCCGTGAGAAACTACCATCATTGCGAGCTGAGATAAAAGATGAT 5 0.125 No Hit GTTTTGTGCATAAAACGACTATCAACCTCACAGACTGGCATTAGTGTCTT 5 0.125 No Hit GCAAGACAACCGCATGTTATTCTATCCTGTCCAAAACTGATTCAGAAATT 5 0.125 No Hit ATAAAGGATGATTTCAGCAAAAGATATGGTGGTGGGAAAGCAGCTACTGC 5 0.125 No Hit GTTTTACTGGAGTTTGCTAATATTTTCTAATTACCTAGATTTCACAGTTT 5 0.125 No Hit GTCCAATTCACCAGGTCTTTTCGACCATCTTTTAGTAAATGATGACCTTG 5 0.125 No Hit CGAAGATCTTTGTGATCAAGCTGGCATCACTCATAGGCGGAAGCATTTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530479 spots for ERR5262792.sra Written 1530479 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra Read 1530478 spots for ERR5262792.sra Written 1530478 spots for ERR5262792.sra SRR ids: ['ERR5262792.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_mar3oy3u ERR5262792.sra spots: 30609561 blocks: [[1, 1530478], [1530479, 3060956], [3060957, 4591434], [4591435, 6121912], [6121913, 7652390], [7652391, 9182868], [9182869, 10713346], [10713347, 12243824], [12243825, 13774302], [13774303, 15304780], [15304781, 16835258], [16835259, 18365736], [18365737, 19896214], [19896215, 21426692], [21426693, 22957170], [22957171, 24487648], [24487649, 26018126], [26018127, 27548604], [27548605, 29079082], [29079083, 30609561]] ERR5262792 file size 9913783 ERR5262792 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262792 ERR5262792_1.fastq ERR5262792_2.fastq Input file: ERR5262792_1.fastq Paired file: ERR5262792_2.fastq trimmed: ERR5262792-trimmed-pair1.fastq, ERR5262792-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:44:33 2024 >> started Fri Dec 6 11:46:36 2024 >> done (123.177s) 30609561 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 1 ( 0.00%) empty read pairs filtered out after trimming by size control 30609560 (100.00%) read pairs available; of these: 11273 ( 0.04%) trimmed read pairs available after processing 30598287 (99.96%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 1 0.00% 23 1 0.00% 24 3 0.00% 25 0 0.00% 26 3 0.00% 27 0 0.00% 28 2 0.00% 29 2 0.00% 30 0 0.00% 31 0 0.00% 32 1 0.00% 33 0 0.00% 34 0 0.00% 35 0 0.00% 36 0 0.00% 37 3 0.00% 38 0 0.00% 39 1 0.00% 40 0 0.00% 41 1 0.00% 42 0 0.00% 43 2 0.00% 44 1 0.00% 45 1 0.00% 46 5 0.00% 47 4 0.00% 48 0 0.00% 49 696 0.00% 50 818 0.00% 51 878 0.00% 52 914 0.00% 53 1123 0.00% 54 1097 0.00% 55 1165 0.00% 56 1278 0.00% 57 1505 0.00% 58 1835 0.01% 59 2218 0.01% 60 2572 0.01% 61 3017 0.01% 62 3226 0.01% 63 3659 0.01% 64 3838 0.01% 65 4195 0.01% 66 4486 0.01% 67 4927 0.02% 68 5799 0.02% 69 6573 0.02% 70 7381 0.02% 71 9047 0.03% 72 9974 0.03% 73 11339 0.04% 74 12466 0.04% 75 13199 0.04% 76 14207 0.05% 77 14977 0.05% 78 16626 0.05% 79 18515 0.06% 80 20392 0.07% 81 22999 0.08% 82 26049 0.09% 83 28885 0.09% 84 30682 0.10% 85 33219 0.11% 86 34105 0.11% 87 35727 0.12% 88 37990 0.12% 89 39659 0.13% 90 43359 0.14% 91 46345 0.15% 92 49870 0.16% 93 54313 0.18% 94 58100 0.19% 95 59881 0.20% 96 61292 0.20% 97 61772 0.20% 98 62690 0.20% 99 65516 0.21% 100 67610 0.22% 101 70727 0.23% 102 74872 0.24% 103 78412 0.26% 104 81509 0.27% 105 84026 0.27% 106 85383 0.28% 107 84417 0.28% 108 85957 0.28% 109 86488 0.28% 110 88592 0.29% 111 91483 0.30% 112 95143 0.31% 113 97657 0.32% 114 102677 0.34% 115 103791 0.34% 116 104124 0.34% 117 105243 0.34% 118 104322 0.34% 119 104682 0.34% 120 104586 0.34% 121 106638 0.35% 122 108610 0.35% 123 112750 0.37% 124 116907 0.38% 125 117403 0.38% 126 119789 0.39% 127 119224 0.39% 128 118860 0.39% 129 119005 0.39% 130 117740 0.38% 131 117857 0.39% 132 120146 0.39% 133 123176 0.40% 134 125804 0.41% 135 128425 0.42% 136 130585 0.43% 137 129352 0.42% 138 128653 0.42% 139 130511 0.43% 140 130336 0.43% 141 133538 0.44% 142 136701 0.45% 143 137068 0.45% 144 141289 0.46% 145 140558 0.46% 146 146138 0.48% 147 275476 0.90% 148 132554 0.43% 149 130801 0.43% 150 23855538 77.93% 30609560 reads passed initial QC criterion=sequence-density sequence-density=1.19 sequence-density-rank=1 fanout-score=6.28 fanout-score-rank=25 prefix-density=6.89 prefix-fanout=1.1 sequence=TTGCCGTTCATCCCCTCGATGGCCGCCTGCATCGACTCCTCGGTGGCGAACGTCACGAACCCGAACCCGCGCGACCGCCCAGTCTCCCT criterion=fanout-score sequence-density=0.03 sequence-density-rank=25 fanout-score=168.85 fanout-score-rank=1 prefix-density=1.05 prefix-fanout=5.2 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTGGTGACTCCACGGACACCGTTGGCGATATAGGGCAG criterion=sequence-density sequence-density=7.15 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=32 prefix-density=7.09 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.09 sequence-density-rank=23 fanout-score=79.83 fanout-score-rank=1 prefix-density=2.06 prefix-fanout=3.7 sequence=AAGAAGAAGAAGGAAGAGTGCTTATCGGCAGCAGCTGGGGTTGTCCGTAAGGGCCCGTGGACGGAGCAGGAGGACATGAAGCTGGTATGGTTCGTGCGGCTCTTCGGAGAGCGCCGCTGGGATTTCTTAGCTAAGGTCTCAGGTTTGCAAGGTGGAGGGTGAAAGGTTTCTCCCCATCCATGCATATATCTCGAGCTCGATCGATCGAGCCATGCGTGCATGGATGGGGGAAACCATGAATGAAGCTAGCTAGCCATGATCTGTCCAAACTAACACTCTTATTGCTCTCTCCGCCGGCATGCACCTTTGTTGTGCTGCTGTCTTGTATTTTGCTACATCTGAATATGCAGGGCTGAACCGGAGTGGGAAGAGCTGCCGTCTCCGGTGGGTGAACTACCTGCACCCGGACCTGAAGCGGGGGCGGATGAGCCCCGACGAGGAGCGGCTCGTCGTCGAGCTCCATGCCAAGTGGGGCAACCGCTGGTCCCGCATCGCCAGGAGCATGCCCGGC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TTGCCGTTCATCCCCTCGATGGCCGCCTGCATCGACTCCTCGGTGGCGAACGTCACGAACCCGAACCCGCGCGACCGCCCAGTCTCCCT -y CGGTTCCGGTTC -o ERR5262792 ERR5262792_1.fastq ERR5262792_2.fastq Input file: ERR5262792_1.fastq Paired file: ERR5262792_2.fastq trimmed: ERR5262792-trimmed-pair1.fastq, ERR5262792-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TTGCCGTTCATCCCCTCGATGGCCGCCTGCATCGACTCCTCGGTGGCGAACGTCACGAACCCGA -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:48:58 2024 >> started Fri Dec 6 11:49:16 2024 >> done (18.146s) 18365736 read pairs processed; of these: 286 ( 0.00%) short read pairs filtered out after trimming by size control 2707 ( 0.01%) empty read pairs filtered out after trimming by size control 18362743 (99.98%) read pairs available; of these: 221 ( 0.00%) trimmed read pairs available after processing 18362522 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 1 0.00% 23 1 0.00% 24 2 0.00% 25 0 0.00% 26 2 0.00% 27 0 0.00% 28 1 0.00% 29 1 0.00% 30 0 0.00% 31 0 0.00% 32 1 0.00% 33 0 0.00% 34 0 0.00% 35 0 0.00% 36 0 0.00% 37 3 0.00% 38 0 0.00% 39 1 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 2 0.00% 44 0 0.00% 45 1 0.00% 46 3 0.00% 47 2 0.00% 48 0 0.00% 49 380 0.00% 50 423 0.00% 51 588 0.00% 52 630 0.00% 53 783 0.00% 54 746 0.00% 55 792 0.00% 56 881 0.00% 57 1034 0.01% 58 1246 0.01% 59 1481 0.01% 60 1761 0.01% 61 1743 0.01% 62 1728 0.01% 63 1986 0.01% 64 2164 0.01% 65 2864 0.02% 66 3027 0.02% 67 2679 0.01% 68 3633 0.02% 69 4448 0.02% 70 4786 0.03% 71 4459 0.02% 72 5897 0.03% 73 7597 0.04% 74 6624 0.04% 75 7308 0.04% 76 8240 0.04% 77 10260 0.06% 78 8731 0.05% 79 11100 0.06% 80 12531 0.07% 81 13113 0.07% 82 14678 0.08% 83 18685 0.10% 84 18382 0.10% 85 20051 0.11% 86 18305 0.10% 87 21327 0.12% 88 24083 0.13% 89 24519 0.13% 90 26506 0.14% 91 27681 0.15% 92 31342 0.17% 93 33270 0.18% 94 33939 0.18% 95 37923 0.21% 96 36079 0.20% 97 37295 0.20% 98 36123 0.20% 99 38680 0.21% 100 42483 0.23% 101 42682 0.23% 102 46672 0.25% 103 45415 0.25% 104 47486 0.26% 105 50863 0.28% 106 51136 0.28% 107 51290 0.28% 108 50732 0.28% 109 50967 0.28% 110 52806 0.29% 111 55048 0.30% 112 55673 0.30% 113 57260 0.31% 114 60939 0.33% 115 62122 0.34% 116 63443 0.35% 117 62173 0.34% 118 62819 0.34% 119 61680 0.34% 120 63785 0.35% 121 63253 0.34% 122 64962 0.35% 123 67629 0.37% 124 70136 0.38% 125 70547 0.38% 126 71308 0.39% 127 71467 0.39% 128 71030 0.39% 129 71613 0.39% 130 70415 0.38% 131 70582 0.38% 132 71463 0.39% 133 73424 0.40% 134 75722 0.41% 135 77053 0.42% 136 78284 0.43% 137 77531 0.42% 138 77389 0.42% 139 78104 0.43% 140 78117 0.43% 141 80044 0.44% 142 81949 0.45% 143 82857 0.45% 144 84527 0.46% 145 84548 0.46% 146 88345 0.48% 147 166422 0.91% 148 79678 0.43% 149 78382 0.43% 150 14313935 77.95% criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=4.07 fanout-score-rank=31 prefix-density=0.29 prefix-fanout=3.0 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.02 sequence-density-rank=35 fanout-score=507.76 fanout-score-rank=1 prefix-density=0.78 prefix-fanout=15.2 sequence=CGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGC criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=2.14 fanout-score-rank=40 prefix-density=0.67 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.09 sequence-density-rank=27 fanout-score=216.94 fanout-score-rank=1 prefix-density=0.97 prefix-fanout=19.7 sequence=CGCCGCCGCCGTC ERR5262792 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:50:33 Started mapping on | Dec 06 11:50:33 Finished on | Dec 06 11:52:53 Mapping speed, Million of reads per hour | 787.03 Number of input reads | 30606567 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 29279624 Uniquely mapped reads % | 95.66% Average mapped length | 286.44 Number of splices: Total | 26441134 Number of splices: Annotated (sjdb) | 24345506 Number of splices: GT/AG | 26042410 Number of splices: GC/AG | 340583 Number of splices: AT/AC | 18109 Number of splices: Non-canonical | 40032 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.00% Deletion average length | 1.56 Insertion rate per base | 0.00% Insertion average length | 1.13 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 267643 % of reads mapped to multiple loci | 0.87% Number of reads mapped to too many loci | 1042 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.44% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1059300 1059300 1059300 N_multimapping 267643 267643 267643 N_noFeature 1462148 28413078 1767599 N_ambiguous 653977 3986 92894 UnstrandedReadsAssigned:27163499 PositiveStrandReadsAssigned:862560 NegativeStrandReadsAssigned:27419131 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=146 echo kmer=141 ERR5262792 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262792-trimmed-pair1.fastq ERR5262792-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,606,567 reads, 27,889,174 reads pseudoaligned [quant] estimated average fragment length: 241.285 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,205 rounds 52973 ERR5262792.ke.tsv 35125 ERR5262792.se.tsv 88098 total ==> ERR5262792.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 696.37 0 0 PNS24247 1044 803.715 208.916 13.6808 PNS24249 1928 1687.72 538.052 16.779 PNS24246 1044 803.715 208.916 13.6808 PNS24248 1044 803.715 208.916 13.6808 PNS24244 1471 1230.72 164.201 7.022 PNS24243 293 113.105 1 0.465328 KQK14069 1603 1362.72 65881.4 2544.48 KQK14071 474 256.804 1606.91 329.331 ==> ERR5262792.se.tsv <== BRADI_1g14170v3 70150 BRADI_1g53295v3 393 BRADI_1g59795v3 1196 BRADI_1g07683v3 0 BRADI_1g00485v3 23 BRADI_1g20270v3 643 BRADI_1g74790v3 1979 BRADI_1g09890v3 0 BRADI_1g77505v3 310 BRADI_1g48960v3 0 ERR5262792 completed mapping pipeline successfully