Starting /dee2/code/volunteer_pipeline.sh ERR5262793 current disk space = 1551324520448 free memory = 1603080176 ERR5262793 SRAfilesize 89f9f250ca8eb3e828246cc7ff706777 ERR5262793.sra ERR5262793.sra file validated ERR5262793 is paired end ERR5262793 is conventional basespace ERR5262793 read1 length is 66-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262793_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 66-150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.573 37.0 37.0 37.0 37.0 37.0 2 36.4835 37.0 37.0 37.0 37.0 37.0 3 36.575 37.0 37.0 37.0 37.0 37.0 4 36.6025 37.0 37.0 37.0 37.0 37.0 5 36.6785 37.0 37.0 37.0 37.0 37.0 6 36.698 37.0 37.0 37.0 37.0 37.0 7 36.6065 37.0 37.0 37.0 37.0 37.0 8 36.7175 37.0 37.0 37.0 37.0 37.0 9 36.6795 37.0 37.0 37.0 37.0 37.0 10-14 36.621 37.0 37.0 37.0 37.0 37.0 15-19 36.5548 37.0 37.0 37.0 37.0 37.0 20-24 36.4976 37.0 37.0 37.0 37.0 37.0 25-29 36.454899999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.4615 37.0 37.0 37.0 37.0 37.0 35-39 36.366200000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.37670000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.3297 37.0 37.0 37.0 37.0 37.0 50-54 36.222500000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.1638 37.0 37.0 37.0 37.0 37.0 60-64 36.1697 37.0 37.0 37.0 37.0 37.0 65-69 36.01340052650731 37.0 37.0 37.0 37.0 37.0 70-74 35.994787736424925 37.0 37.0 37.0 37.0 37.0 75-79 35.89343878096359 37.0 37.0 37.0 37.0 37.0 80-84 35.957138616178135 37.0 37.0 37.0 37.0 37.0 85-89 35.9514811395053 37.0 37.0 37.0 37.0 37.0 90-94 35.854265622355705 37.0 37.0 37.0 37.0 37.0 95-99 35.81864602155686 37.0 37.0 37.0 37.0 37.0 100-104 35.742783888454355 37.0 37.0 37.0 37.0 37.0 105-109 35.85361067405193 37.0 37.0 37.0 37.0 37.0 110-114 35.724524119341496 37.0 37.0 37.0 37.0 37.0 115-119 35.81740272028702 37.0 37.0 37.0 37.0 37.0 120-124 35.79407382119176 37.0 37.0 37.0 37.0 37.0 125-129 35.865245138857475 37.0 37.0 37.0 37.0 37.0 130-134 35.68273286856542 37.0 37.0 37.0 37.0 37.0 135-139 35.596571707171314 37.0 37.0 37.0 37.0 37.0 140-144 35.569808795789946 37.0 37.0 37.0 37.0 37.0 145-149 35.4111766004166 37.0 37.0 37.0 37.0 37.0 150 35.485390578413835 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 3.0 21 3.0 22 3.0 23 6.0 24 3.0 25 4.0 26 2.0 27 5.0 28 8.0 29 16.0 30 25.0 31 34.0 32 65.0 33 109.0 34 189.0 35 421.0 36 2778.0 37 324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.125 12.9 7.875 42.1 2 19.634817408704354 10.855427713856928 34.26713356678339 35.24262131065532 3 13.5 17.299999999999997 23.549999999999997 45.65 4 15.15 25.174999999999997 24.6 35.075 5 16.375 27.325 29.549999999999997 26.75 6 22.275 30.45 23.1 24.175 7 13.775 20.65 46.550000000000004 19.025 8 13.200000000000001 26.05 29.025000000000002 31.724999999999998 9 14.7 20.7 34.175 30.425 10-14 16.475 28.689999999999998 26.290000000000003 28.544999999999998 15-19 17.0 26.369999999999997 26.174999999999997 30.455 20-24 18.14 26.43 25.91 29.520000000000003 25-29 18.565 29.87 26.51 25.055 30-34 18.495 28.87 26.384999999999998 26.25 35-39 17.895 30.955 25.865 25.285000000000004 40-44 18.740000000000002 28.89 25.94 26.43 45-49 18.360000000000003 31.180000000000003 26.05 24.41 50-54 19.689999999999998 32.005 25.555 22.75 55-59 19.475 34.44 23.36 22.725 60-64 19.685 31.974999999999998 24.495 23.845 65-69 18.70374074814963 31.926385277055413 25.91018203640728 23.459691938387678 70-74 20.38630904723779 33.60688550840673 23.984187349879903 22.02261809447558 75-79 21.867174196133426 32.966042271862165 23.770409696484023 21.396373835520386 80-84 19.792449992480073 30.490800621647367 26.17436205945756 23.542387326415 85-89 21.20375483158476 33.13588675267306 24.44154409919181 21.218814316550375 90-94 24.640088593576966 29.593274942112153 25.36494513238699 20.401691331923892 95-99 19.66491192549099 32.79003846932577 25.207531889046365 22.33751771613687 100-104 19.95619620027505 32.41990526154943 24.52503438088932 23.098864157286204 105-109 21.596051007815714 32.16269025092554 24.074454956807898 22.166803784450842 110-114 20.17516583747927 32.69589552238806 24.512852404643446 22.61608623548922 115-119 23.052339288520983 29.988997747157754 25.83433750720386 21.12432545711741 120-124 23.237000372558413 31.03411570599819 22.002235350471018 23.72664857097238 125-129 23.694735704248192 29.169819478975246 23.138039130904765 23.997405685871797 130-134 22.28577714222858 29.798702012979874 24.458255417445827 23.457265427345728 135-139 23.234765010119183 29.71666291882168 25.252979536766357 21.795592534292783 140-144 20.68886469138633 27.749441228723708 26.459969052667777 25.101725027222187 145-149 20.290110406389473 29.022785999530186 24.47733145407564 26.2097721400047 150 23.822301729278472 25.491949910554563 26.62492546213476 24.0608228980322 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.5 10 1.5 11 0.0 12 1.0 13 1.0 14 0.0 15 0.5 16 1.5 17 1.5 18 1.0 19 0.5 20 0.0 21 0.0 22 0.5 23 0.5 24 2.0 25 3.5 26 2.5 27 7.5 28 11.5 29 13.5 30 28.0 31 42.0 32 62.0 33 92.5 34 129.5 35 145.0 36 127.5 37 114.0 38 146.0 39 160.0 40 140.0 41 198.0 42 249.0 43 227.5 44 291.5 45 259.0 46 150.5 47 136.0 48 115.0 49 88.0 50 63.0 51 51.0 52 32.5 53 24.5 54 62.0 55 216.0 56 191.0 57 52.0 58 101.0 59 97.5 60 58.0 61 40.0 62 6.0 63 3.5 64 2.5 65 1.5 66 6.0 67 5.0 68 2.0 69 4.5 70 9.5 71 10.0 72 8.5 73 6.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 1.0 70-71 1.0 72-73 1.0 74-75 2.0 76-77 1.0 78-79 2.0 80-81 2.0 82-83 1.0 84-85 1.0 86-87 6.0 88-89 1.0 90-91 4.0 92-93 11.0 94-95 9.0 96-97 7.0 98-99 11.0 100-101 10.0 102-103 20.0 104-105 13.0 106-107 13.0 108-109 12.0 110-111 10.0 112-113 12.0 114-115 15.0 116-117 27.0 118-119 22.0 120-121 24.0 122-123 30.0 124-125 20.0 126-127 20.0 128-129 30.0 130-131 25.0 132-133 22.0 134-135 44.0 136-137 28.0 138-139 25.0 140-141 22.0 142-143 34.0 144-145 46.0 146-147 27.0 148-149 33.0 150-151 3354.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.225 #Duplication Level Percentage of deduplicated Percentage of total 1 44.91593274619696 14.025000000000002 2 21.77742193755004 13.600000000000001 3 10.168134507606084 9.525 4 6.725380304243394 8.4 5 4.083266613290633 6.375 6 2.8823058446757406 5.4 7 1.8414731785428344 4.025 8 1.521216973578863 3.8 9 1.200960768614892 3.375 >10 4.643714971977582 24.925 >50 0.16012810248198558 3.75 >100 0.08006405124099279 2.8000000000000003 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAG 112 2.8000000000000003 No Hit CCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 86 2.15 No Hit GGCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCC 64 1.6 No Hit CTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGA 37 0.9249999999999999 No Hit GGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAG 34 0.8500000000000001 No Hit CCCACATCCAACACCATGCAGAGTGTTCATGGACAGTCTTCTTTTTCTCC 32 0.8 No Hit CCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACAT 32 0.8 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 32 0.8 No Hit CCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACAT 32 0.8 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 27 0.675 No Hit CCCAGCTCTGTCTTTTTTTAATCATCATCAAGGCTATTGATGATTTTATC 26 0.65 No Hit GGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAA 26 0.65 No Hit GGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCC 26 0.65 No Hit ATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTAT 25 0.625 No Hit CCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACAC 24 0.6 No Hit GCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACA 23 0.575 No Hit CTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATG 23 0.575 No Hit CGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTC 21 0.525 No Hit GCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCT 20 0.5 No Hit CTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAA 18 0.44999999999999996 No Hit TAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAG 18 0.44999999999999996 No Hit GCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACAC 18 0.44999999999999996 No Hit CTTCATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTTTAACAAA 18 0.44999999999999996 No Hit GCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATAT 17 0.42500000000000004 No Hit GCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGA 16 0.4 No Hit CTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAG 16 0.4 No Hit CTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTT 16 0.4 No Hit CCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 16 0.4 No Hit CTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCAT 16 0.4 No Hit CTATTTTTTCCTCAAATAATCATCTCGCTGAGCTGGATTGAGTCCTAAAC 15 0.375 No Hit GTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCT 15 0.375 No Hit GGCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTC 15 0.375 No Hit CCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAAC 15 0.375 No Hit GTCAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAAT 14 0.35000000000000003 No Hit ACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCA 14 0.35000000000000003 No Hit GCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCC 14 0.35000000000000003 No Hit GGTGACTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTTAAT 13 0.325 No Hit CTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCG 13 0.325 No Hit GCCCTTAAAATGTTTTAGAACATGAACACTTGTAGGAGATGATTGGATGG 13 0.325 No Hit CCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCA 13 0.325 No Hit GGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAAT 13 0.325 No Hit CCAGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCA 12 0.3 No Hit CATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACG 12 0.3 No Hit CTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGACCT 12 0.3 No Hit CTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCACTTAA 12 0.3 No Hit ACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATA 12 0.3 No Hit CGACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTC 12 0.3 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 12 0.3 No Hit CTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTT 12 0.3 No Hit CCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 12 0.3 No Hit CCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATA 11 0.27499999999999997 No Hit AGCTAGGGTAAATGCATTGCACCATTGTGAAACGAGAGGCCAATGGCCGA 11 0.27499999999999997 No Hit GTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATA 11 0.27499999999999997 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 10 0.25 No Hit CGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCT 10 0.25 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 10 0.25 No Hit GTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTG 10 0.25 No Hit CACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTG 10 0.25 No Hit GTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAAT 10 0.25 No Hit CTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAAAAAGAGAAA 10 0.25 No Hit CATCAAACGTATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTG 10 0.25 No Hit ACCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATAC 9 0.22499999999999998 No Hit CTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGA 9 0.22499999999999998 No Hit CCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTT 9 0.22499999999999998 No Hit GGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCA 9 0.22499999999999998 No Hit CGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGT 9 0.22499999999999998 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 9 0.22499999999999998 No Hit TTTTTTTTTAGAATAGCAAGTTCACAGATCAGATTAAACCATGCAAGTTT 9 0.22499999999999998 No Hit CGGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCATGC 9 0.22499999999999998 No Hit CATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGC 9 0.22499999999999998 No Hit CTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGCCAAAA 9 0.22499999999999998 No Hit CACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCG 9 0.22499999999999998 No Hit GTGGTAGCTCTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAA 9 0.22499999999999998 No Hit CTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 9 0.22499999999999998 No Hit ATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGA 9 0.22499999999999998 No Hit GACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTA 9 0.22499999999999998 No Hit CACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCA 8 0.2 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTT 8 0.2 No Hit GTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAG 8 0.2 No Hit CCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTCAC 8 0.2 No Hit GTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGGGTAA 8 0.2 No Hit CTCCAGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTG 8 0.2 No Hit GGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTCAG 8 0.2 No Hit TGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGA 8 0.2 No Hit CTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCCTCAAATAA 8 0.2 No Hit CTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAACACGGCGTCTC 8 0.2 No Hit CCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGGGTAAAACGA 8 0.2 No Hit CTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTTTTCATGCCG 8 0.2 No Hit GCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTCA 8 0.2 No Hit CTTTGGATGGCAAACTACGTTATCAACAACAGTGCCGGGAGGGACATTAT 8 0.2 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATACGAAACA 8 0.2 No Hit CCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGC 8 0.2 No Hit ATGTAGTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCT 8 0.2 No Hit CCATATTCTTCATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTT 8 0.2 No Hit GCCCCTTCCCTGAGCCATATCGACCGGCCGGGACCTTTGGTTCAGGAAGA 8 0.2 No Hit CTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 7 0.17500000000000002 No Hit GGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCTTGTGCGATA 7 0.17500000000000002 No Hit GCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCA 7 0.17500000000000002 No Hit AGAGAAAACGACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTT 7 0.17500000000000002 No Hit GGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCT 7 0.17500000000000002 No Hit CCCAGTGACTGACTCCTTACATAAATTAACTTAATTGAAAAATGACAAAG 7 0.17500000000000002 No Hit ATCATGTAGTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCC 7 0.17500000000000002 No Hit AGAAAATTATCGATTTGTTTGAGATCTACAAGATTAGAACTTTGTTCTAG 7 0.17500000000000002 No Hit AGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACA 7 0.17500000000000002 No Hit CTTCCCTGAGCCATATCGACCGGCCGGGACCTTTGGTTCAGGAAGAGAAA 7 0.17500000000000002 No Hit GTTAGGTGGTAGCTCTCCACTTAAACCACAACTATCAATATACCTGGAAT 7 0.17500000000000002 No Hit TGGCAAACTACGTTATCAACAACAGTGCCGGGAGGGACATTATCCGGTGA 7 0.17500000000000002 No Hit ACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTAC 7 0.17500000000000002 No Hit GGGCAGTTTACAATCTTCAGGTTCTGTAGTTCAGGAAACAAAGTTCTCAG 7 0.17500000000000002 No Hit GTCGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCC 7 0.17500000000000002 No Hit CTGCACTTCTGACTCTTTATCCTGTATTTTTCTGAGGATCTTATCTTCAA 7 0.17500000000000002 No Hit CCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAGT 7 0.17500000000000002 No Hit GCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAG 7 0.17500000000000002 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 7 0.17500000000000002 No Hit CCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACAC 7 0.17500000000000002 No Hit GCCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCA 7 0.17500000000000002 No Hit ATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGC 7 0.17500000000000002 No Hit CACATTTTTTAAGTTTAACTACATCTTTTGCAATCAAGCAATATTTGTCC 7 0.17500000000000002 No Hit GGTAGCTCTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAAAA 6 0.15 No Hit GGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACAA 6 0.15 No Hit GTTCCCACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACA 6 0.15 No Hit CTTTCATCCAAAAATCTGACAAAACTCCAAAGTCCTGTCTCATCTGCAGC 6 0.15 No Hit CATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGC 6 0.15 No Hit GGAATGTTATTGCCATCAGTAGATTCGCCAAAAAAGTGCTCCCGGTTCAG 6 0.15 No Hit GTACTTTGTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAGATATTCT 6 0.15 No Hit CAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCAC 6 0.15 No Hit GCACTGATTTCATCTCGTGGATGTGAATTGATGGATTCTCTCTTAATGAT 6 0.15 No Hit GCCACACATGAGATCATAGTACGAACACGGAGAGAGGGTAAAACGAAGGG 6 0.15 No Hit GTCTTGGATATGGAGGAACTGTTAATCATGATTAGATTTTTTTTTTCAAA 6 0.15 No Hit ACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTC 6 0.15 No Hit CAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACT 6 0.15 No Hit CCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGATCATAG 6 0.15 No Hit GCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGCGGAT 6 0.15 No Hit TACCATTACCACCACTTTCTTCCTCCTCTAGCGCTGTTTCAAAGTAAATA 6 0.15 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 6 0.15 No Hit CCTGTCTCATCTGCAGCAAAGATCATAGGATTGCAGTCAAATCCAACACC 6 0.15 No Hit CCATGATATCGTCAGCCTCGTACTCCATTGTACAATCACCAGTATAGAAT 6 0.15 No Hit GTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGC 6 0.15 No Hit CCCTGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 6 0.15 No Hit CCCGCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 6 0.15 No Hit GTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAA 6 0.15 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 6 0.15 No Hit GACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCC 6 0.15 No Hit GCCTTTTTTTGCTACAGAGTGTGCAAATGACTTTACCCTCCTGGTCCTTC 6 0.15 No Hit TCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 6 0.15 No Hit GCCATAGTATGGCTCGGGCACAGACCACCTTGCACTACGATATATCCCAC 6 0.15 No Hit GTACAATTCTGAGGTTTATCAGGTCAACCTAACAGTCTGAAACATTGCAT 6 0.15 No Hit CTATCCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTC 6 0.15 No Hit GCTCTCTCTGCAGAGAATTTATCTTTAGTCCAGCTTCTCCAGTTCGCCCA 6 0.15 No Hit GGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAATTCGGAATGTTAT 6 0.15 No Hit CTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAG 6 0.15 No Hit GTGACTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTTAATT 6 0.15 No Hit CCACAACTATCAATATACCTGGAATAAAAAAAAGAGAAATGGTTATGCTC 6 0.15 No Hit GGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCATGCA 6 0.15 No Hit GCACCATTGTGAAACGAGAGGCCAATGGCCGACGATATAGAGGTCCTTGG 5 0.125 No Hit CATAAATTAACTTAATTGAAAAATGACAAAGTAGAGAAAACGACTCGAGT 5 0.125 No Hit TCTGTCTTTTTTTAATCATCATCAAGGCTATTGATGATTTTATCGTTGCA 5 0.125 No Hit GTTCTTTGGATGGCAAACTACGTTATCAACAACAGTGCCGGGAGGGACAT 5 0.125 No Hit TGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGCCAA 5 0.125 No Hit CAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAGA 5 0.125 No Hit ATTTTAACAAAGTATAGTATCCCCTATTTTTTCCTCAAATAATCATCTCG 5 0.125 No Hit CTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCACTTAACTA 5 0.125 No Hit GCCGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAG 5 0.125 No Hit TCCACGAGTTCAGGGCAATCTGTAATTTCGACAGCCTCTAGACAAGAGAA 5 0.125 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACA 5 0.125 No Hit ATCGGTGACTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTT 5 0.125 No Hit GGCACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCTT 5 0.125 No Hit GGAATGATAAACAGAAGGGGAGCTGAGAAAAAACAAAAACGTAAGTGGAT 5 0.125 No Hit CCAGTATAGAATAAATTGTGAACTGTGACCTTGATCTAGTCCTATAGTTC 5 0.125 No Hit CCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACA 5 0.125 No Hit CAGTAAACTCGTTATCTGATGCCCATAGTATTTTCAGGTTCTTAAGTTTT 5 0.125 No Hit AGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGC 5 0.125 No Hit ACAAGCAAAACCATATTCTTCATTTTAGATCATGTAGTCTGGCAAATTCT 5 0.125 No Hit CCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGG 5 0.125 No Hit CACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACATTCCC 5 0.125 No Hit CTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTT 5 0.125 No Hit ACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTT 5 0.125 No Hit GCCTACAGAAAATTATCGATTTGTTTGAGATCTACAAGATTAGAACTTTG 5 0.125 No Hit GGTACTCGAGGCTCCTCTTGAGACCATGACGAAAAAACTGGGGGTAATCT 5 0.125 No Hit GTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCG 5 0.125 No Hit AACAAGCAAAACCATATTCTTCATTTTAGATCATGTAGTCTGGCAAATTC 5 0.125 No Hit CCCTATTTTTTCCTCAAATAATCATCTCGCTGAGCTGGATTGAGTCCTAA 5 0.125 No Hit CATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTA 5 0.125 No Hit CACCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATA 5 0.125 No Hit CCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAGGGTAAAAC 5 0.125 No Hit GTCCAGCTTCTCCAGTTCGCCCATCCTTTCTATGATTGCCTTTTTGCTTG 5 0.125 No Hit CCCCCTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTT 5 0.125 No Hit CTTCAGGTTCTGTAGTTCAGGAAACAAAGTTCTCAGGTTCTTCTTCTCTG 5 0.125 No Hit CTTAAAATGTTTTAGAACATGAACACTTGTAGGAGATGATTGGATGGAAC 5 0.125 No Hit GCACTTCTGACTCTTTATCCTGTATTTTTCTGAGGATCTTATCTTCAACA 5 0.125 No Hit TTTTTTTTTAAACGTTTGAGCCACAAATTTATTCAGATACCAGTATATTC 5 0.125 No Hit GGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGT 5 0.125 No Hit CCTTGCACTACGATATATCCCACATCCAACACCATGCAGAGTGTTCATGG 5 0.125 No Hit GTACTGCCTACCAGTTGGCCTTTAGCTCATAAAAACATAGGAGCTAGCAT 5 0.125 No Hit CCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATACG 5 0.125 No Hit CTGCCCCTTCCCTGAGCCATATCGACCGGCCGGGACCTTTGGTTCAGGAA 5 0.125 No Hit ATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGA 5 0.125 No Hit GGCATGTCATGCCTTTTCGCTTGCATTAATAACTTACTGGGCATGTGGAA 5 0.125 No Hit GCCTCGTACTCCATTGTACAATCACCAGTATAGAATAAATTGTGAACTGT 5 0.125 No Hit GGTACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCC 5 0.125 No Hit CCTAACAGTCTGAAACATTGCATGTCAAGAATCTAGGCTAATAGAATTAG 5 0.125 No Hit CATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACG 5 0.125 No Hit TAATAAGCTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTA 5 0.125 No Hit CCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATGAG 5 0.125 No Hit CCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR5262793 read2 length is 66-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262793_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 66-150 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0675 37.0 37.0 37.0 37.0 37.0 2 35.8015 37.0 37.0 37.0 37.0 37.0 3 35.7285 37.0 37.0 37.0 37.0 37.0 4 35.8845 37.0 37.0 37.0 37.0 37.0 5 35.9835 37.0 37.0 37.0 37.0 37.0 6 35.9995 37.0 37.0 37.0 37.0 37.0 7 35.9905 37.0 37.0 37.0 37.0 37.0 8 36.035 37.0 37.0 37.0 37.0 37.0 9 35.9555 37.0 37.0 37.0 37.0 37.0 10-14 36.0741 37.0 37.0 37.0 37.0 37.0 15-19 36.0316 37.0 37.0 37.0 37.0 37.0 20-24 36.0286 37.0 37.0 37.0 37.0 37.0 25-29 35.9859 37.0 37.0 37.0 37.0 37.0 30-34 35.9039 37.0 37.0 37.0 37.0 37.0 35-39 35.904599999999995 37.0 37.0 37.0 37.0 37.0 40-44 35.8739 37.0 37.0 37.0 37.0 37.0 45-49 35.9132 37.0 37.0 37.0 37.0 37.0 50-54 35.7961 37.0 37.0 37.0 37.0 37.0 55-59 35.7397 37.0 37.0 37.0 37.0 37.0 60-64 35.739799999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.73254017571426 37.0 37.0 37.0 37.0 37.0 70-74 35.7154934166164 37.0 37.0 37.0 37.0 37.0 75-79 35.64667152261947 37.0 37.0 37.0 37.0 37.0 80-84 35.594461343470236 37.0 37.0 37.0 37.0 37.0 85-89 35.55103384159574 37.0 37.0 37.0 37.0 37.0 90-94 35.51730537080903 37.0 37.0 37.0 37.0 37.0 95-99 35.47610679632806 37.0 37.0 37.0 37.0 37.0 100-104 35.37039614176764 37.0 37.0 37.0 34.6 37.0 105-109 35.32794528090089 37.0 37.0 37.0 32.2 37.0 110-114 35.291057406902404 37.0 37.0 37.0 32.2 37.0 115-119 35.289951193428884 37.0 37.0 37.0 32.2 37.0 120-124 35.295901500554315 37.0 37.0 37.0 32.2 37.0 125-129 35.24702500207174 37.0 37.0 37.0 32.2 37.0 130-134 35.10469006992839 37.0 37.0 37.0 25.0 37.0 135-139 35.05478313236678 37.0 37.0 37.0 25.0 37.0 140-144 34.99470371329078 37.0 37.0 37.0 25.0 37.0 145-149 34.89536088329629 37.0 37.0 37.0 25.0 37.0 150 34.72971360381862 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 2.0 13 2.0 14 0.0 15 2.0 16 0.0 17 0.0 18 1.0 19 0.0 20 3.0 21 1.0 22 4.0 23 5.0 24 10.0 25 7.0 26 8.0 27 11.0 28 21.0 29 28.0 30 37.0 31 41.0 32 81.0 33 175.0 34 314.0 35 707.0 36 2358.0 37 178.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.800000000000004 24.925 8.15 27.125 2 28.775000000000002 24.2 29.9 17.125 3 19.6 26.6 32.0 21.8 4 25.724999999999998 28.025 24.65 21.6 5 25.575 33.45 19.6 21.375 6 23.400000000000002 37.4 19.325 19.875 7 23.075000000000003 19.325 35.85 21.75 8 21.675 23.7 24.85 29.775000000000002 9 23.65 21.525 28.9 25.924999999999997 10-14 23.810000000000002 26.915 25.485000000000003 23.79 15-19 25.290000000000003 25.745 25.715 23.25 20-24 25.655 25.72 25.82 22.805 25-29 24.175 25.130000000000003 26.51 24.185000000000002 30-34 24.555 26.545 26.705000000000002 22.195 35-39 24.759999999999998 26.275 27.265 21.7 40-44 24.245 25.575 26.895000000000003 23.285 45-49 24.665 24.785 26.640000000000004 23.91 50-54 24.055 26.474999999999998 26.939999999999998 22.53 55-59 23.974999999999998 26.52 26.805 22.7 60-64 23.369999999999997 25.295 28.87 22.465 65-69 24.78495699139828 25.89017803560712 26.630326065213044 22.694538907781556 70-74 25.035028022417933 25.17514011208967 27.066653322658123 22.72317854283427 75-79 25.16778523489933 24.94240208354202 27.33647200240409 22.553340679154562 80-84 24.519977941545097 24.780668772246454 27.638241339549808 23.061111946658645 85-89 24.075096631695196 25.244716630691233 28.070879975904823 22.60930676170875 90-94 24.822516489602737 24.99874125169931 27.974422234529982 22.204320024167966 95-99 25.5618546264426 25.541607612877097 27.768779105081997 21.1277586555983 100-104 25.974224440935256 24.25755183128725 27.48713769038765 22.281086037389844 105-109 26.083826176394957 24.196451529956285 27.287220365132427 22.432501928516327 110-114 24.75124378109453 26.653192371475953 26.927860696517413 21.667703150912107 115-119 25.091767173571057 26.114315679077084 27.367593078133197 21.42632406921867 120-124 25.263831148065236 25.67956507834986 27.5450378424475 21.511565931137405 125-129 25.886869997836904 26.02206359506814 27.060350421804024 21.03071598529094 130-134 26.86255089688566 25.94915813799934 26.3618355893034 20.826455375811598 135-139 25.553116027698024 26.10482463547824 26.740978438326856 21.601080898496875 140-144 24.86897425560099 27.437654783159594 27.120889247249902 20.572481713989518 145-149 24.805699481865283 26.524964672633068 27.77319830428639 20.89613754121526 150 26.103818615751788 24.731503579952268 28.520286396181383 20.644391408114558 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.0 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.5 24 1.0 25 1.0 26 0.5 27 0.5 28 1.0 29 3.5 30 12.0 31 20.5 32 23.5 33 36.0 34 60.5 35 86.0 36 89.5 37 102.0 38 120.0 39 119.0 40 127.0 41 141.5 42 171.5 43 189.5 44 203.5 45 196.5 46 179.5 47 172.0 48 163.0 49 149.0 50 141.5 51 147.0 52 129.0 53 103.0 54 84.5 55 108.5 56 161.5 57 173.5 58 139.5 59 94.5 60 73.0 61 66.0 62 49.5 63 37.0 64 28.5 65 19.5 66 11.0 67 8.0 68 10.5 69 9.5 70 4.0 71 3.5 72 5.5 73 5.0 74 5.0 75 4.0 76 1.5 77 1.5 78 1.0 79 0.5 80 0.5 81 0.5 82 1.0 83 1.5 84 1.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 1.0 70-71 1.0 72-73 1.0 74-75 2.0 76-77 1.0 78-79 2.0 80-81 2.0 82-83 1.0 84-85 1.0 86-87 6.0 88-89 2.0 90-91 4.0 92-93 11.0 94-95 8.0 96-97 7.0 98-99 11.0 100-101 10.0 102-103 21.0 104-105 13.0 106-107 12.0 108-109 12.0 110-111 10.0 112-113 12.0 114-115 15.0 116-117 34.0 118-119 23.0 120-121 21.0 122-123 26.0 124-125 21.0 126-127 20.0 128-129 29.0 130-131 26.0 132-133 22.0 134-135 46.0 136-137 30.0 138-139 33.0 140-141 26.0 142-143 38.0 144-145 31.0 146-147 24.0 148-149 31.0 150-151 3352.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.85 #Duplication Level Percentage of deduplicated Percentage of total 1 56.26880641925778 28.050000000000004 2 21.51454363089268 21.45 3 9.829488465396189 14.7 4 5.566700100300903 11.1 5 2.908726178535607 7.249999999999999 6 1.4543630892678034 4.35 7 1.0030090270812437 3.5000000000000004 8 0.4513540621865597 1.7999999999999998 9 0.05015045135406219 0.22499999999999998 >10 0.9528585757271816 7.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAG 29 0.7250000000000001 No Hit GTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGC 23 0.575 No Hit AGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTA 22 0.5499999999999999 No Hit GTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGG 20 0.5 No Hit GTCGTCGATCTCGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCC 18 0.44999999999999996 No Hit GTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCG 18 0.44999999999999996 No Hit GGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGA 16 0.4 No Hit GCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGT 16 0.4 No Hit GTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCC 16 0.4 No Hit CTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTTC 14 0.35000000000000003 No Hit AGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCG 14 0.35000000000000003 No Hit GATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTG 14 0.35000000000000003 No Hit TGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTTTATGGG 13 0.325 No Hit GTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTA 13 0.325 No Hit GGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCG 12 0.3 No Hit GATGCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGT 12 0.3 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 11 0.27499999999999997 No Hit GGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTT 11 0.27499999999999997 No Hit CTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAA 11 0.27499999999999997 No Hit GTTCAGACTTCGACGGTGCAAAGCACTGCAAGTCTAGAGAGCTTGGATAA 9 0.22499999999999998 No Hit CCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTT 8 0.2 No Hit CCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTC 8 0.2 No Hit CTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTT 8 0.2 No Hit AACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGA 8 0.2 No Hit GGAAGAGAGAGGATGATTGGACCCCTGTTTTGGAGCAAGGCAGGCCAGTC 8 0.2 No Hit GGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCGG 8 0.2 No Hit AATTGATTAATACGGCGGCCGCGTCGTCGATCTCGCTGCAAGCCTGCAAG 8 0.2 No Hit GTTTCTGAGTTTTGTGGAAGTCTTTTTGCCTAGCTTGTTTCTGTACTTCT 8 0.2 No Hit GTGGCGGGCGCCCCGCACTGCCTCGCCTTCATGTTCGTCAACTGGACCTT 8 0.2 No Hit GGTGAAGCTTTTCCTGGACGAGAACTTCAAGCAAGTGGGTACTCCATTGA 7 0.17500000000000002 No Hit TTTTCTTCCTGGGCATGAAGAACTCCATTCCCAAGCGACAGGCCAAGAAG 7 0.17500000000000002 No Hit ACCAAAGTTTACAAGGAGAAAGAGCTACGGTGATACTCCGCCAAACGGAG 7 0.17500000000000002 No Hit GGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTC 7 0.17500000000000002 No Hit CATCAGGCCTCTTCCAATCCTTTTGCCGGCTCGTACATGCCCAGCAGCGG 7 0.17500000000000002 No Hit AGGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCG 7 0.17500000000000002 No Hit AAATAATGGTGGAAAATCTTATGGCAAGCTGGCAATCTATTCAAGACATT 7 0.17500000000000002 No Hit GGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGG 7 0.17500000000000002 No Hit GAGTTGCAACAGATTGTGAAGAAGCGAAAAAGGGAGAGGCTTCAAATTGA 7 0.17500000000000002 No Hit ATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTGC 7 0.17500000000000002 No Hit AGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCA 7 0.17500000000000002 No Hit GCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCT 7 0.17500000000000002 No Hit AGAGAGGCTCGCCCGCGCGCGTGATGAATTGATTAATACGGCGGCCGCGT 7 0.17500000000000002 No Hit AGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGC 7 0.17500000000000002 No Hit CGTCGATCTCGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCCTT 7 0.17500000000000002 No Hit GGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCGGGGGA 7 0.17500000000000002 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 7 0.17500000000000002 No Hit ATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGGTCCACCGCTCCA 7 0.17500000000000002 No Hit GTAGCCTGGTGCCCCGCGCTAAACTAAAAAGATCTGTGAAATTGGAATCG 7 0.17500000000000002 No Hit AGCAAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCG 7 0.17500000000000002 No Hit AGTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATG 6 0.15 No Hit GGGGACAAAAGGAGACGTTTCTGTACTGAAACCCACTCTTATGATTTCAG 6 0.15 No Hit CTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTTCTCTT 6 0.15 No Hit ACAACACTCGCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCA 6 0.15 No Hit ACTCAAACAGGACCTTCCATGTGAAGTAATAGGCTAATACGGAGTAGCTT 6 0.15 No Hit GTCAGCTCCATGCCCGCAACACGTACACTGGAACGGGGATGATGTAGAGA 6 0.15 No Hit GTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTT 6 0.15 No Hit AGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACC 6 0.15 No Hit GTTGTGTTTGCACAGTGAGCCAGTGAGGTAGCCTGGTGCCCCGCGCTAAA 6 0.15 No Hit CTTATGATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCAGTGTT 6 0.15 No Hit GTAGCGCCGTAGCGGTGACTAATTCTGCGCATGTACCTAGCCAACTACTT 6 0.15 No Hit CCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTT 6 0.15 No Hit GCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGC 6 0.15 No Hit GTTGCTTTGTCTTGATCATTGATCAGTCGGTATTTTTGTTGTCCCTTTTT 6 0.15 No Hit CATAAGAGACGCAGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAA 6 0.15 No Hit GCTCCATGCCCGCAACACGTACACTGGAACGGGGATGATGTAGAGATGCT 6 0.15 No Hit AGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGG 6 0.15 No Hit AAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGC 6 0.15 No Hit GGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAG 6 0.15 No Hit ATCTCTGGCAGATAAATGACAAGGTATCTTGCCCAGATGAGCTGATAGCC 6 0.15 No Hit CCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTT 6 0.15 No Hit AGAGGGGGAGCTTTTTGAGACAGAGAAGGAGACTACCAAGAATCTGCCTG 6 0.15 No Hit ACAAAATGTAGAAAGTAGATATGTTCAGGACAGAATGAGTGGGATAGTCA 6 0.15 No Hit GATTAATACGGCGGCCGCGTCGTCGATCTCGCTGCAAGCCTGCAAGCTAT 6 0.15 No Hit CCTCGGGGGAAGGATCAAGAGAGGCTCGCCCGCGCGCGTGATGAATTGAT 6 0.15 No Hit CGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGCCG 6 0.15 No Hit CTCATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTT 6 0.15 No Hit GCTTCCAGGAAATGGCACAAGGAGTTGTGTTTGCACAGTGAGCCAGTGAG 6 0.15 No Hit AGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGT 6 0.15 No Hit ATCGAGGTTTGCAGGTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGA 5 0.125 No Hit GTCTGATTTAAAAAGCTACCATACCTGTTCCGTTGTGGTCAAATCTTATG 5 0.125 No Hit GGAGATTCACCTAATTTAGCACCTGGCTTTGATGAAGCAAACCCTCCATA 5 0.125 No Hit GAAACACTTGACAACATTATCTTTCCATGCGGTTGAGGAGTTGGGCAAAA 5 0.125 No Hit AGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTCC 5 0.125 No Hit GGCCATGATTGGTGGACTAGATGCAGTTGACATGTTACTGGAACATATGC 5 0.125 No Hit AGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATT 5 0.125 No Hit CACGTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTA 5 0.125 No Hit AAGGTAAATAAGCGTTTTCTTTTCCTTCTGCTTTACTGCCTTTTGCAGAT 5 0.125 No Hit GGACGCACGGATCGAGGAAGAGAGAGGATGATTGGACCCCTGTTTTGGAG 5 0.125 No Hit AGATATTGCAGTCTCTGGACGAATCTGTCACAGATCCTGATGAAAAGTAT 5 0.125 No Hit ATTTTTTCCTATGACTGGAATATGAAAACTTCTTTGTACTGGAGTTGAAC 5 0.125 No Hit AGAAAACTTGACCCAACTTGAGCAACTGTTATGTCTTGGTTATGCTTGAC 5 0.125 No Hit GGTTGATGGGCCATGATTGGTGGACTAGATGCAGTTGACATGTTACTGGA 5 0.125 No Hit ACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTGCCCTT 5 0.125 No Hit CTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGAT 5 0.125 No Hit GCCAGTTCATCTTGGAGAAATAATGGTGGAAAATCTTATGGCAAGCTGGC 5 0.125 No Hit GGAATTTTCTTCCAGAGATGGGGAAATAGAGGCCGTTCTGAAAGATATAT 5 0.125 No Hit CTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAAAAGGA 5 0.125 No Hit GAAAACAATTGAACAAAAGCAGCCTTGATATTGTGCCCCAGACATCATAT 5 0.125 No Hit CTGATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTA 5 0.125 No Hit AAGGAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGAC 5 0.125 No Hit CCGTACTCCCGGGATCTGCAGTTTTTTGCCCTTCGTTTTACCCTCTCTCC 5 0.125 No Hit AAGTTGTAATTTTGAGTGGGTGCGCCATTTGGAAGATGGCTCCGTAAAAT 5 0.125 No Hit GTCAGGACAATCATTCCTAAACTTGGCACGGGAGACGTTTATCTGGCATA 5 0.125 No Hit GTTGACATGTTACTGGAACATATGCATCTTTTCTTATGCATGAGGGTGCT 5 0.125 No Hit CGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACT 5 0.125 No Hit GGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAG 5 0.125 No Hit GCAAGCTCCAAGCGTAATCCCTGGAAGTTTTGACCGGGTTGTAGAGGCTG 5 0.125 No Hit CAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCT 5 0.125 No Hit ATGAAGAGCTATAACCATCAGTGTTGATGCCTCTCTCACACACAAATGAA 5 0.125 No Hit CCTGTTTTGGAGCAAGGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCT 5 0.125 No Hit CAAGAACCCCAATCCAAAGCCCAACCCGAAAAAATTCCAATAAATTCTCC 5 0.125 No Hit CTTATGGTCAGCTCCATGCCCGCAACACGTACACTGGAACGGGGATGATG 5 0.125 No Hit CTATTACGGTTCATGCCAATCGATAACCTCTATTTTACAGATCATCCTGG 5 0.125 No Hit GAAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTT 5 0.125 No Hit TGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAG 5 0.125 No Hit CTGGAGCAGAGATCCGCTGGTCGAACACAGAAGATGCTGATTCTCCTCTG 5 0.125 No Hit GTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTG 5 0.125 No Hit CAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTC 5 0.125 No Hit GCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCC 5 0.125 No Hit CCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGGGCTCGTA 5 0.125 No Hit CATCAGCACCAACAAGTTCGTCGGCCCACTTCCTGAAGTAGTAGAAAACT 5 0.125 No Hit ATCTCATAGCACTGGGCATCAGCACCAACAAGTTCGTCGGCCCACTTCCT 5 0.125 No Hit GTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTACAT 5 0.125 No Hit GTAAAAATGGCAGAAGATATGGTGGCAAGGACTGCAATGTCCTGTAACGT 5 0.125 No Hit AGAACTTCAAGCAAGTGGGTACTCCATTGATGGAACCACAAGTTGTAATT 5 0.125 No Hit TTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGAG 5 0.125 No Hit AGTCATCAGCGCTTCTATATTCATGTCCAGGGGCTAAACAAACTACTACC 5 0.125 No Hit AAGTACTTTGCTAGGGACAAGAAGGCAAGGGCAAAGAAGACAGAGGGGGA 5 0.125 No Hit GCATTGGGAACCCTAAAGATGCCAACAAAGCGCAATGCTCGCCAAAGAAT 5 0.125 No Hit AAATCATCTTAGCTGAGCACCTGAACCCCCCCTAAATAACCGAAACAAGG 5 0.125 No Hit AGCGAAAACTTCCAAACATTGAAAAAGCTAAAATTAGTTAACATACCAAA 5 0.125 No Hit GGCAGAGCTGAAAAGAAAGAAGCTGAGGACAAATATTGCTTGATTGCAAA 5 0.125 No Hit CTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTACATTTTTT 5 0.125 No Hit GTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTAT 5 0.125 No Hit GTCTCTGTGACTTTATGTGTGGACTTAAGTGTTATTGGGCTCCTCGTTTA 5 0.125 No Hit GTGAAATTGGAATCGTGTTTAGGACTCAATCCAGCTCAGCGAGATGATTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.025 62-63 0.0 0.0 0.0 0.0 0.05 64-65 0.0 0.0 0.0 0.0 0.05 66-67 0.0 0.0 0.0 0.0 0.05 68-69 0.0 0.0 0.0 0.0 0.05 70-71 0.0 0.0 0.0 0.0 0.05 72-73 0.0 0.0 0.0 0.0 0.05 74-75 0.0 0.0 0.0 0.0 0.05 76-77 0.0 0.0 0.0 0.0 0.05 78-79 0.0 0.0 0.0 0.0 0.05 80-81 0.0 0.0 0.0 0.0 0.05 82-83 0.0 0.0 0.0 0.0 0.05 84-85 0.0 0.0 0.0 0.0 0.05 86-87 0.0 0.0 0.0 0.0 0.05 88-89 0.0 0.0 0.0 0.0 0.05 90-91 0.0 0.0 0.0 0.0 0.05 92-93 0.0 0.0 0.0 0.0 0.05 94-95 0.0 0.0 0.0 0.0 0.05 96-97 0.0 0.0 0.0 0.0 0.05 98-99 0.0 0.0 0.0 0.0 0.05 100-101 0.0 0.0 0.0 0.0 0.05 102-103 0.0 0.0 0.0 0.0 0.05 104-105 0.0 0.0 0.0 0.0 0.05 106-107 0.0 0.0 0.0 0.0 0.05 108-109 0.0 0.0 0.0 0.0 0.05 110-111 0.0 0.0 0.0 0.0 0.05 112-113 0.0 0.0 0.0 0.0 0.05 114-115 0.0 0.0 0.0 0.0 0.05 116-117 0.0 0.0 0.0 0.0 0.05 118-119 0.0 0.0 0.0 0.0 0.05 120-121 0.0 0.0 0.0 0.0 0.05 122-123 0.0 0.0 0.0 0.0 0.05 124-125 0.0 0.0 0.0 0.0 0.05 126-127 0.0 0.0 0.0 0.0 0.05 128-129 0.0 0.0 0.0 0.0 0.05 130-131 0.0 0.0 0.0 0.0 0.05 132-133 0.0 0.0 0.0 0.0 0.05 134-135 0.0 0.0 0.0 0.0 0.05 136-137 0.0 0.0 0.0 0.0 0.05 138 0.0 0.0 0.0 0.0 0.05 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGAGCT 10 0.007371881 141.34999 6 >>END_MODULE Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580400 spots for ERR5262793.sra Written 1580400 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra Read 1580388 spots for ERR5262793.sra Written 1580388 spots for ERR5262793.sra SRR ids: ['ERR5262793.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jlbulw5w ERR5262793.sra spots: 31607772 blocks: [[1, 1580388], [1580389, 3160776], [3160777, 4741164], [4741165, 6321552], [6321553, 7901940], [7901941, 9482328], [9482329, 11062716], [11062717, 12643104], [12643105, 14223492], [14223493, 15803880], [15803881, 17384268], [17384269, 18964656], [18964657, 20545044], [20545045, 22125432], [22125433, 23705820], [23705821, 25286208], [25286209, 26866596], [26866597, 28446984], [28446985, 30027372], [30027373, 31607772]] ERR5262793 file size 10310760 ERR5262793 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262793 ERR5262793_1.fastq ERR5262793_2.fastq Input file: ERR5262793_1.fastq Paired file: ERR5262793_2.fastq trimmed: ERR5262793-trimmed-pair1.fastq, ERR5262793-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:44:47 2024 >> started Fri Dec 6 11:45:32 2024 >> done (44.732s) 31607772 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 31607772 (100.00%) read pairs available; of these: 9574 ( 0.03%) trimmed read pairs available after processing 31598198 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 0 0.00% 22 1 0.00% 23 0 0.00% 24 1 0.00% 25 1 0.00% 26 0 0.00% 27 0 0.00% 28 2 0.00% 29 2 0.00% 30 2 0.00% 31 0 0.00% 32 1 0.00% 33 3 0.00% 34 0 0.00% 35 0 0.00% 36 4 0.00% 37 2 0.00% 38 2 0.00% 39 4 0.00% 40 1 0.00% 41 0 0.00% 42 2 0.00% 43 2 0.00% 44 5 0.00% 45 2 0.00% 46 2 0.00% 47 3 0.00% 48 1 0.00% 49 380 0.00% 50 424 0.00% 51 514 0.00% 52 514 0.00% 53 556 0.00% 54 613 0.00% 55 690 0.00% 56 700 0.00% 57 762 0.00% 58 996 0.00% 59 1145 0.00% 60 1330 0.00% 61 1469 0.00% 62 1720 0.01% 63 1967 0.01% 64 2196 0.01% 65 2289 0.01% 66 2524 0.01% 67 2909 0.01% 68 3152 0.01% 69 3788 0.01% 70 4387 0.01% 71 5262 0.02% 72 5969 0.02% 73 6626 0.02% 74 7242 0.02% 75 8492 0.03% 76 9197 0.03% 77 9863 0.03% 78 10721 0.03% 79 12155 0.04% 80 13328 0.04% 81 15368 0.05% 82 16921 0.05% 83 19097 0.06% 84 20894 0.07% 85 23069 0.07% 86 24331 0.08% 87 26377 0.08% 88 27951 0.09% 89 29408 0.09% 90 32012 0.10% 91 34105 0.11% 92 36538 0.12% 93 40295 0.13% 94 43162 0.14% 95 44718 0.14% 96 46951 0.15% 97 49254 0.16% 98 50885 0.16% 99 52962 0.17% 100 55272 0.17% 101 57038 0.18% 102 60251 0.19% 103 63473 0.20% 104 65971 0.21% 105 68816 0.22% 106 72421 0.23% 107 73450 0.23% 108 74608 0.24% 109 76518 0.24% 110 77475 0.25% 111 80924 0.26% 112 83142 0.26% 113 85663 0.27% 114 90282 0.29% 115 92635 0.29% 116 94317 0.30% 117 95335 0.30% 118 97755 0.31% 119 98478 0.31% 120 98978 0.31% 121 100722 0.32% 122 102418 0.32% 123 104702 0.33% 124 108193 0.34% 125 109229 0.35% 126 111324 0.35% 127 113402 0.36% 128 113949 0.36% 129 115518 0.37% 130 115458 0.37% 131 115820 0.37% 132 118977 0.38% 133 119886 0.38% 134 120314 0.38% 135 124193 0.39% 136 125591 0.40% 137 125557 0.40% 138 125720 0.40% 139 129533 0.41% 140 129454 0.41% 141 130541 0.41% 142 132339 0.42% 143 131393 0.42% 144 134272 0.42% 145 134631 0.43% 146 138681 0.44% 147 260544 0.82% 148 132241 0.42% 149 131123 0.41% 150 25531023 80.77% 31607772 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.78 fanout-score-rank=36 prefix-density=0.30 prefix-fanout=2.4 sequence=CCAGAGCTTGAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=42 fanout-score=118.29 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=3.1 sequence=TCTTGGTTGACGGCGATGACCTCCCCATTGCTGAGTATGTCCTTTGTCTGCTGGCTCATCGTGCGCACGTCACACCCGATCAGAAGAGGAGCCTTGGCAAGGGCCCAGATGCTGAAGTGTGAACGGTACTCAGCATCAGACATCCCGCCGTTCCCCACTTCAAGCATGTCAGGATCATTCCATCCACCAGGTTCA criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.15 fanout-score-rank=40 prefix-density=0.27 prefix-fanout=2.1 sequence=CTCTGGAACACC criterion=fanout-score sequence-density=0.09 sequence-density-rank=11 fanout-score=79.33 fanout-score-rank=1 prefix-density=0.75 prefix-fanout=9.6 sequence=GAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATTGTTGTTCTCCTGCTCATCGTGCTTTCGGTTTGTGCCGCAGGAGGAAGGGAGCTGGCGGAACAAAAGCTACAAAAGGACTTCTACAGTGCTGCATCCAAAGAGGGAGCAACAGTTTCGAGCAACCATCCAAGGAACCTCATGGTTAAGACGAACGACTA ERR5262793 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:46:09 Started mapping on | Dec 06 11:46:09 Finished on | Dec 06 11:48:04 Mapping speed, Million of reads per hour | 989.46 Number of input reads | 31607772 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 30880891 Uniquely mapped reads % | 97.70% Average mapped length | 289.10 Number of splices: Total | 29450715 Number of splices: Annotated (sjdb) | 27199894 Number of splices: GT/AG | 29006967 Number of splices: GC/AG | 388342 Number of splices: AT/AC | 19604 Number of splices: Non-canonical | 35802 Mismatch rate per base, % | 0.13% Deletion rate per base | 0.00% Deletion average length | 1.51 Insertion rate per base | 0.00% Insertion average length | 1.13 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 287325 % of reads mapped to multiple loci | 0.91% Number of reads mapped to too many loci | 1097 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.36% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 439556 439556 439556 N_multimapping 287325 287325 287325 N_noFeature 1557234 29983983 1860049 N_ambiguous 693815 4163 99155 UnstrandedReadsAssigned:28629842 PositiveStrandReadsAssigned:892745 NegativeStrandReadsAssigned:28921687 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262793 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262793-trimmed-pair1.fastq ERR5262793-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,607,772 reads, 29,225,541 reads pseudoaligned [quant] estimated average fragment length: 264.195 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,206 rounds 52973 ERR5262793.ke.tsv 35125 ERR5262793.se.tsv 88098 total ==> ERR5262793.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 673.584 0 0 PNS24247 1044 780.805 214.405 13.7565 PNS24249 1928 1664.8 421.617 12.6873 PNS24246 1044 780.805 214.405 13.7565 PNS24248 1044 780.805 214.405 13.7565 PNS24244 1471 1207.8 273.167 11.3305 PNS24243 293 107.034 0 0 KQK14069 1603 1339.8 66850.1 2499.63 KQK14071 474 243.625 1299.78 267.278 ==> ERR5262793.se.tsv <== BRADI_1g14170v3 71965 BRADI_1g53295v3 337 BRADI_1g59795v3 1357 BRADI_1g07683v3 0 BRADI_1g00485v3 31 BRADI_1g20270v3 766 BRADI_1g74790v3 2119 BRADI_1g09890v3 0 BRADI_1g77505v3 378 BRADI_1g48960v3 0 ERR5262793 completed mapping pipeline successfully