Starting /dee2/code/volunteer_pipeline.sh ERR5262794 current disk space = 1551348162560 free memory = 1606810860 ERR5262794 SRAfilesize ff92e3b14e41be0f9dec004bf51fd06b ERR5262794.sra ERR5262794.sra file validated ERR5262794 is paired end ERR5262794 is conventional basespace ERR5262794 read1 length is 52-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262794_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52-150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5735 37.0 37.0 37.0 37.0 37.0 2 36.47025 37.0 37.0 37.0 37.0 37.0 3 36.618 37.0 37.0 37.0 37.0 37.0 4 36.6075 37.0 37.0 37.0 37.0 37.0 5 36.6555 37.0 37.0 37.0 37.0 37.0 6 36.6075 37.0 37.0 37.0 37.0 37.0 7 36.529 37.0 37.0 37.0 37.0 37.0 8 36.741 37.0 37.0 37.0 37.0 37.0 9 36.574 37.0 37.0 37.0 37.0 37.0 10-14 36.5466 37.0 37.0 37.0 37.0 37.0 15-19 36.517500000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.4584 37.0 37.0 37.0 37.0 37.0 25-29 36.425 37.0 37.0 37.0 37.0 37.0 30-34 36.4123 37.0 37.0 37.0 37.0 37.0 35-39 36.32280000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.261199999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.24060000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.197612578144536 37.0 37.0 37.0 37.0 37.0 55-59 36.21500375093773 37.0 37.0 37.0 37.0 37.0 60-64 36.142485621405356 37.0 37.0 37.0 37.0 37.0 65-69 36.06496624156039 37.0 37.0 37.0 37.0 37.0 70-74 36.05006251562891 37.0 37.0 37.0 37.0 37.0 75-79 35.95088054154609 37.0 37.0 37.0 37.0 37.0 80-84 35.95537768884442 37.0 37.0 37.0 37.0 37.0 85-89 35.93785634373354 37.0 37.0 37.0 37.0 37.0 90-94 35.80300225168877 37.0 37.0 37.0 37.0 37.0 95-99 35.85303977983487 37.0 37.0 37.0 37.0 37.0 100-104 35.7742807105329 37.0 37.0 37.0 37.0 37.0 105-109 35.822426016104686 37.0 37.0 37.0 37.0 37.0 110-114 35.67646487460134 37.0 37.0 37.0 37.0 37.0 115-119 35.71463786289358 37.0 37.0 37.0 37.0 37.0 120-124 35.68232045498436 37.0 37.0 37.0 37.0 37.0 125-129 35.68068867068866 37.0 37.0 37.0 37.0 37.0 130-134 35.56521140149815 37.0 37.0 37.0 37.0 37.0 135-139 35.52984722154629 37.0 37.0 37.0 37.0 37.0 140-144 35.557944494437294 37.0 37.0 37.0 37.0 37.0 145-149 35.44949256287686 37.0 37.0 37.0 37.0 37.0 150 35.422374429223744 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 3.0 23 1.0 24 4.0 25 5.0 26 8.0 27 13.0 28 13.0 29 30.0 30 30.0 31 50.0 32 57.0 33 90.0 34 175.0 35 460.0 36 2749.0 37 310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.375 10.6 8.1 38.925 2 20.375469336670836 9.436795994993743 35.99499374217772 34.1927409261577 3 16.45 15.525 28.499999999999996 39.525 4 19.725 24.625 23.674999999999997 31.974999999999998 5 21.275 27.800000000000004 26.224999999999998 24.7 6 19.275000000000002 34.1 23.3 23.325000000000003 7 14.6 25.374999999999996 42.075 17.95 8 16.0 28.025 32.95 23.025000000000002 9 16.125 27.575 32.574999999999996 23.724999999999998 10-14 16.939999999999998 29.435 28.599999999999998 25.025 15-19 17.4 26.125 29.945 26.529999999999998 20-24 17.23 26.040000000000003 27.250000000000004 29.48 25-29 19.314999999999998 29.03 28.055000000000003 23.599999999999998 30-34 17.035 25.91 29.065 27.99 35-39 17.97 28.005000000000003 27.355 26.669999999999998 40-44 17.18 25.779999999999998 28.33 28.71 45-49 16.295 28.07 29.615000000000002 26.02 50-54 18.696869686968697 29.322932293229325 27.77777777777778 24.202420242024203 55-59 18.064516129032256 31.00275068767192 26.081520380095025 24.851212803200802 60-64 18.43460865216304 29.122280570142532 30.172543135783947 22.27056764191048 65-69 17.57939484871218 27.95198799699925 31.50787696924231 22.960740185046262 70-74 21.6254063515879 30.7776944236059 26.60165041260315 20.99524881220305 75-79 23.249299719887954 29.796918767507 26.65566226490596 20.298119247699077 80-84 20.795397698849424 30.560280140070034 27.86893446723362 20.775387693846923 85-89 20.239167417192032 30.391273891724207 26.318422896027222 23.051135795056542 90-94 24.17312984738554 28.68651488616462 26.22466850137603 20.915686765073804 95-99 18.10858143607706 31.458593945459096 28.326244683512634 22.106579934951213 100-104 21.391043282461847 29.70227670753065 25.058794095571677 23.847885914435825 105-109 21.194508467782345 30.078164144703877 26.410461970137288 22.31686541737649 110-114 21.41274796093042 30.83274594703454 25.143490081562785 22.61101601047226 115-119 20.272805638659296 31.03290908168957 25.30297652248872 23.391308757162417 120-124 21.8077474892396 29.503996720639474 24.877023980323838 23.81123180979709 125-129 21.898188988635773 30.8494629235639 22.567588604639095 24.684759483161226 130-134 21.60780179230364 31.51818661043753 23.241960991038482 23.632050606220346 135-139 20.259587650892 30.50421963465442 26.0976391411174 23.138553573336182 140-144 20.404281910558062 31.56550562408303 24.68075857197196 23.349453893386947 145-149 19.68490692980489 31.559766763848394 22.342453464902444 26.41287284144427 150 21.575342465753426 34.18949771689498 25.371004566210047 18.864155251141554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.5 12 0.5 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.0 24 0.0 25 1.5 26 4.0 27 21.0 28 27.0 29 17.0 30 22.5 31 47.0 32 78.0 33 80.0 34 91.0 35 107.0 36 111.0 37 105.0 38 140.5 39 202.5 40 185.5 41 199.5 42 259.5 43 302.0 44 324.0 45 288.0 46 212.0 47 188.5 48 149.5 49 97.0 50 82.5 51 40.5 52 14.5 53 10.0 54 11.0 55 46.0 56 74.5 57 71.5 58 97.0 59 90.5 60 65.0 61 45.5 62 16.0 63 9.0 64 7.5 65 5.5 66 5.0 67 3.5 68 3.0 69 5.0 70 6.0 71 8.5 72 6.0 73 2.0 74 3.0 75 3.5 76 1.5 77 1.5 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 52-53 1.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 1.0 106-107 9.0 108-109 11.0 110-111 1.0 112-113 9.0 114-115 14.0 116-117 15.0 118-119 13.0 120-121 20.0 122-123 20.0 124-125 17.0 126-127 22.0 128-129 24.0 130-131 33.0 132-133 17.0 134-135 20.0 136-137 12.0 138-139 29.0 140-141 20.0 142-143 45.0 144-145 73.0 146-147 23.0 148-149 45.0 150-151 3504.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.225 #Duplication Level Percentage of deduplicated Percentage of total 1 46.51721377101681 14.524999999999999 2 20.97678142514011 13.100000000000001 3 11.128903122497999 10.424999999999999 4 6.00480384307446 7.5 5 3.4427542033626897 5.375 6 1.521216973578863 2.85 7 2.321857485988791 5.075 8 1.0408326661329064 2.6 9 0.8006405124099278 2.25 >10 6.084867894315452 33.375 >50 0.16012810248198558 2.9250000000000003 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCC 59 1.4749999999999999 No Hit CCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 58 1.4500000000000002 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 46 1.15 No Hit TAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAG 44 1.0999999999999999 No Hit CTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATG 41 1.0250000000000001 No Hit GCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGC 41 1.0250000000000001 No Hit GCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATAT 38 0.95 No Hit CTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACATG 32 0.8 No Hit GCTATACATAGGTTAGTACCACTGGAATTTCAACTAAAACTATCTATATG 32 0.8 No Hit GTGGCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTG 29 0.7250000000000001 No Hit CTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCT 28 0.7000000000000001 No Hit CCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACAT 27 0.675 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 26 0.65 No Hit CCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 26 0.65 No Hit CTGGAATTTCAACTAAAACTATCTATATGTACCTATTTTATTTACAAGGG 26 0.65 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 24 0.6 No Hit CGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATC 23 0.575 No Hit CCCACATCCAACACCATGCAGAGTGTTCATGGACAGTCTTCTTTTTCTCC 22 0.5499999999999999 No Hit CTCCAATTAAGCTCTTTTTTCAGAATTTTAACAATCTCACGCCTTGGCAC 22 0.5499999999999999 No Hit GGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCA 21 0.525 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 20 0.5 No Hit CATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTA 20 0.5 No Hit GCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCT 20 0.5 No Hit GGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAA 19 0.475 No Hit GCCATCCGACCACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCAT 19 0.475 No Hit CTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGATCCAA 19 0.475 No Hit GGCTATACATAGGTTAGTACCACTGGAATTTCAACTAAAACTATCTATAT 19 0.475 No Hit CCCAGCTCTGTCTTTTTTTAATCATCATCAAGGCTATTGATGATTTTATC 18 0.44999999999999996 No Hit CTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAA 18 0.44999999999999996 No Hit GGACAGTCTTCTTTTTCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAA 18 0.44999999999999996 No Hit CTGGCTATACATAGGTTAGTACCACTGGAATTTCAACTAAAACTATCTAT 18 0.44999999999999996 No Hit CCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACAC 18 0.44999999999999996 No Hit CTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCT 17 0.42500000000000004 No Hit GGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACG 16 0.4 No Hit GCAGAGTGTTCATGGACAGTCTTCTTTTTCTCCAATTAAGCTCTTTTTTC 15 0.375 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 15 0.375 No Hit GCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCC 15 0.375 No Hit CCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTAC 14 0.35000000000000003 No Hit GTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAA 14 0.35000000000000003 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 14 0.35000000000000003 No Hit GTCTTCAAGGACAATCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACC 14 0.35000000000000003 No Hit ATCCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAA 14 0.35000000000000003 No Hit GTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCTTCACGCA 13 0.325 No Hit CTTTCATCCAAAAATCTGACAAAACTCCAAAGTCCTGTCTCATCTGCAGC 13 0.325 No Hit GCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAGA 13 0.325 No Hit CGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTC 13 0.325 No Hit CCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGGAACCTGAGTTGC 13 0.325 No Hit GGGCAGTTTACAATCTTCAGGTTCTGTAGTTCAGGAAACAAAGTTCTCAG 13 0.325 No Hit GGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGA 13 0.325 No Hit CTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGCA 13 0.325 No Hit GCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCAG 13 0.325 No Hit GTGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCT 13 0.325 No Hit GTCTCGGCATCTTTCTCGGCTAAGCCCAAGTACGCTTTGGTGGCCTGTCT 12 0.3 No Hit CCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTCAC 12 0.3 No Hit GGTTAGTACCACTGGAATTTCAACTAAAACTATCTATATGTACCTATTTT 12 0.3 No Hit GGCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTC 12 0.3 No Hit CCTGTCTCATCTGCAGCAAAGATCATAGGATTGCAGTCAAATCCAACACC 12 0.3 No Hit CTTGCACTACGATATATCCCACATCCAACACCATGCAGAGTGTTCATGGA 12 0.3 No Hit CTTGGCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGT 12 0.3 No Hit GGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAG 11 0.27499999999999997 No Hit GGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTT 11 0.27499999999999997 No Hit GGCAGTTTACAATCTTCAGGTTCTGTAGTTCAGGAAACAAAGTTCTCAGG 11 0.27499999999999997 No Hit GTGCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAA 11 0.27499999999999997 No Hit CCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 11 0.27499999999999997 No Hit AGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 11 0.27499999999999997 No Hit GCCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCA 11 0.27499999999999997 No Hit CTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCAGA 11 0.27499999999999997 No Hit GCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCAACAACTCCTCCTT 11 0.27499999999999997 No Hit GCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCTCAAA 10 0.25 No Hit ACCTTGCACTACGATATATCCCACATCCAACACCATGCAGAGTGTTCATG 10 0.25 No Hit GACCAGGTAGCAAAGCCAACCAACAGGGACTGAGGGGCAGGGGCAGAAGC 10 0.25 No Hit GTAGGAAGTTCTCCAACTAACCCGATTCCACTCGCTAAAAAGATTTGCAG 10 0.25 No Hit ATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCAACGTAC 10 0.25 No Hit CACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTG 10 0.25 No Hit CTCTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATT 10 0.25 No Hit GTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGC 10 0.25 No Hit GCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACA 10 0.25 No Hit GCCCAATTACTCACATCAGTTCTGTTACTCTCACCACATCATCTGCCTAA 10 0.25 No Hit CGGCTATACATAGGTTAGTACCACTGGAATTTCAACTAAAACTATCTATA 9 0.22499999999999998 No Hit CACTCTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAA 9 0.22499999999999998 No Hit CCCACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACATTC 9 0.22499999999999998 No Hit GTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTG 9 0.22499999999999998 No Hit GTCTGGAGGTGGCGGCACGCTATTGTATGACGACGGCACGGGGGCGCCGC 9 0.22499999999999998 No Hit CACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCATTTTTACGAAT 9 0.22499999999999998 No Hit CCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAA 9 0.22499999999999998 No Hit GTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCA 9 0.22499999999999998 No Hit CTCACATCAGTTCTGTTACTCTCACCACATCATCTGCCTAAAAAAAACAC 9 0.22499999999999998 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 9 0.22499999999999998 No Hit GCCATCACGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGC 8 0.2 No Hit GGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCAAC 8 0.2 No Hit GTACCATCTTTTTTAATCTTTTTCCTACCAAAGATTGTTTTCATTGATCT 8 0.2 No Hit TTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTT 8 0.2 No Hit ACGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCTTCACG 8 0.2 No Hit GTCAACAAGATTGATTGTGCCATCACTGATGGCTGTGTAAATCTTAACAC 8 0.2 No Hit CCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACACA 8 0.2 No Hit CTACGATATATCCCACATCCAACACCATGCAGAGTGTTCATGGACAGTCT 8 0.2 No Hit GCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAAT 8 0.2 No Hit ATCAGTTTGCCAATGTCTTCTGTCCAGTTATCTGAAGTTTTAAACCTATT 8 0.2 No Hit ATCAGTTTTTGTTTCCCAGCTCTGTCTTTTTTTAATCATCATCAAGGCTA 8 0.2 No Hit GCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCAGGGGCACAA 8 0.2 No Hit GGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCATGCA 8 0.2 No Hit GCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCC 7 0.17500000000000002 No Hit GCTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAG 7 0.17500000000000002 No Hit GGCTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGATCC 7 0.17500000000000002 No Hit GTTACAGACGCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCA 7 0.17500000000000002 No Hit GTTAGTACCACTGGAATTTCAACTAAAACTATCTATATGTACCTATTTTA 7 0.17500000000000002 No Hit CCTGGCCATCTTGGACTTCTGCGCGTTGCCGCCGCCCATGGCTTCTTTCT 7 0.17500000000000002 No Hit GGCAGGCGAACAGCGACCAGGTAGCAAAGCCAACCAACAGGGACTGAGGG 7 0.17500000000000002 No Hit CTAGCGAATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATGA 7 0.17500000000000002 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 7 0.17500000000000002 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 7 0.17500000000000002 No Hit ACTCTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAG 7 0.17500000000000002 No Hit GTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCTCAAAAGCC 7 0.17500000000000002 No Hit GGCATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCA 7 0.17500000000000002 No Hit GTGACGATAAAAATTGTTTTCGTTACAGACGCTTGATACAATGCAATGCC 7 0.17500000000000002 No Hit GCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 7 0.17500000000000002 No Hit ATAGGTTAGTACCACTGGAATTTCAACTAAAACTATCTATATGTACCTAT 7 0.17500000000000002 No Hit CGCTAGCGAATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCAT 7 0.17500000000000002 No Hit GGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGC 7 0.17500000000000002 No Hit CTTAAAATGTTTTAGAACATGAACACTTGTAGGAGATGATTGGATGGAAC 7 0.17500000000000002 No Hit CCCTGATGTGTTGAGTCAACAACTCCTCCTTGAGCCACCTCATCTAACAG 7 0.17500000000000002 No Hit ACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGT 7 0.17500000000000002 No Hit GACCACCTTGCACTACGATATATCCCACATCCAACACCATGCAGAGTGTT 7 0.17500000000000002 No Hit GCTATCCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTT 7 0.17500000000000002 No Hit GGCAACTCCACGAGTTCAGGGCAATCTGTAATTTCGACAGCCTCTAGACA 7 0.17500000000000002 No Hit CTTGACACATCTGGTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTG 7 0.17500000000000002 No Hit CCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTG 7 0.17500000000000002 No Hit GCTTAGGATTTTTCTCTCTGTAAGTATGCTTGAGTTCTGAAAGCCTCGTG 7 0.17500000000000002 No Hit CGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCTTCACGC 7 0.17500000000000002 No Hit GCTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGATCCA 7 0.17500000000000002 No Hit CATCCGACCACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCATTT 6 0.15 No Hit CTTTTTTCTTTTTTTGCTTCAGACGTCTTCGTCCATGCCGTCCTCCTCCA 6 0.15 No Hit AGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTT 6 0.15 No Hit GGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCA 6 0.15 No Hit GTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCAGAACTGAC 6 0.15 No Hit CTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGACCT 6 0.15 No Hit AGGCGAACAGCGACCAGGTAGCAAAGCCAACCAACAGGGACTGAGGGGCA 6 0.15 No Hit CAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATGATGCACCT 6 0.15 No Hit GTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTT 6 0.15 No Hit CGGCTGTGTAAATCTTAACACTCTCCAAAGCAACCATTGAAAGCGCGTGC 6 0.15 No Hit CAAAAATCTGACAAAACTCCAAAGTCCTGTCTCATCTGCAGCAAAGATCA 6 0.15 No Hit GTTAGAACTAAAATTAGATCCATCACATTTTCTTGAAACATCAGCTCTAA 6 0.15 No Hit TCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 6 0.15 No Hit ATCCTCTGGGGCAGTTGACAGAACAATCGAGACATCTGGTTTTTTCTTTT 6 0.15 No Hit CCTTGCACTACGATATATCCCACATCCAACACCATGCAGAGTGTTCATGG 6 0.15 No Hit GTCTTCTTTTTCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAATCTCA 6 0.15 No Hit CCTTGACACATCTGGTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCT 6 0.15 No Hit ACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCATTTTTACGAATT 6 0.15 No Hit TGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTG 6 0.15 No Hit ACATAGGTTAGTACCACTGGAATTTCAACTAAAACTATCTATATGTACCT 5 0.125 No Hit CTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATT 5 0.125 No Hit CCTCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCACA 5 0.125 No Hit ATCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAATCTCACGCCTTGGC 5 0.125 No Hit GGCCTTTTTTTGCTACAGAGTGTGCAAATGACTTTACCCTCCTGGTCCTT 5 0.125 No Hit GACCGGGTAGCAAAGCCAACCAACAGGGACTGAGGGGCAGGGGCAGAAGC 5 0.125 No Hit GTCAGCATCGACTTGTGACGATAAAAATTGTTTTCGTTACAGACGCTTGA 5 0.125 No Hit GCACCCGATCGCTTCAGCAAACTTTTTCTGCTTGAAATACTCATTGCCCT 5 0.125 No Hit TTTTTTTTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTC 5 0.125 No Hit ACCACCTTGCACTACGATATATCCCACATCCAACACCATGCAGAGTGTTC 5 0.125 No Hit GGTCAAAGCTCTGTTTTAAGCAATCAAAACATGCCTTGTATATCAGGACA 5 0.125 No Hit GTTTTTTCTTTTTACTGCTTGGGCTGGCATTTTGCATGTTCCCATCCGAG 5 0.125 No Hit GTGAAACGAGAGGCCAATGGCCGACGATATAGAGGTCCTTGGCAGCACAA 5 0.125 No Hit CTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGA 5 0.125 No Hit GCCACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCATTTTTACGA 5 0.125 No Hit TTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATTG 5 0.125 No Hit GTCCTGTCTCATCTGCAGCAAAGATCATAGGATTGCAGTCAAATCCAACA 5 0.125 No Hit ATCAGGACAGAATAATTAAATTGATAATATATAACAGTTTAATTTACATA 5 0.125 No Hit GTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCAACGTACTGGT 5 0.125 No Hit GTACGCTTTGGTGGCCTGTCTACAGGAAACTGTCGTGGCCCGACATTTTT 5 0.125 No Hit GCCAATGTCTTCTGTCCAGTTATCTGAAGTTTTAAACCTATTATATGGTA 5 0.125 No Hit CAGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACT 5 0.125 No Hit CTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTT 5 0.125 No Hit GTCAGCTGCCCTACACGAACCCTTGACACATCTGGTGGGCATAGAGCGCA 5 0.125 No Hit CTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCATCTTGTTCCATT 5 0.125 No Hit GTTGCAGGATCGAACCTCCTGTATTAACTCTTAAACATCTGACATCTCTA 5 0.125 No Hit CATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCAACGTA 5 0.125 No Hit GACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCA 5 0.125 No Hit GTCCTCAGTTACTTGAAGAGCAAATTTTTGCACCTTAACCCTTTCCTGGA 5 0.125 No Hit CTCTGGGGCAGTTGACAGAACAATCGAGACATCTGGTTTTTTTCTTTTTA 5 0.125 No Hit CCTCTATACATGGAAATTAGTGGCATATATAATAGCTATATACAAACTAT 5 0.125 No Hit GTGCCATCACTGATGGCTGTGTAAATCTTAACACTCTCCAAAGCAACCAT 5 0.125 No Hit GCGACCAGGTAGCAAAGCCAACCAACAGGGACTGAGGGGCAGGGGCAGAA 5 0.125 No Hit CCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGACAG 5 0.125 No Hit GTTCCTTTTACTCCTGCAGTGTTTTATCGGAAAACAGTATCTGCATCCTT 5 0.125 No Hit GAAGGCCCTGATGTGTTGAGTCAACAACTCCTCCTTGAGCCACCTCATCT 5 0.125 No Hit GGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCAACA 5 0.125 No Hit CCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 5 0.125 No Hit GCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTCA 5 0.125 No Hit CCTAAGTTTCTGGTCTCCTCTGAATCCCGTTGCTAGTATTATAATATCAC 5 0.125 No Hit GCAGGATACTTGCTATTTTTGCCAACTTCTTATCCAGATACAGGCAGTTC 5 0.125 No Hit CGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCAACAACTCCTCCT 5 0.125 No Hit CTGTAAAACGATGAATGAAAAGGCTGGGATCTATGAGCTTCTGAACAATT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCAGA 10 0.007422789 141.02501 4 AAAAATT 10 0.007422789 141.02501 9 CGGCCGG 20 0.0028413108 33.677612 140-144 >>END_MODULE ERR5262794 read2 length is 52-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262794_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1275 37.0 37.0 37.0 37.0 37.0 2 35.8485 37.0 37.0 37.0 37.0 37.0 3 35.82 37.0 37.0 37.0 37.0 37.0 4 35.958 37.0 37.0 37.0 37.0 37.0 5 36.0475 37.0 37.0 37.0 37.0 37.0 6 35.9835 37.0 37.0 37.0 37.0 37.0 7 36.023 37.0 37.0 37.0 37.0 37.0 8 36.0425 37.0 37.0 37.0 37.0 37.0 9 36.178 37.0 37.0 37.0 37.0 37.0 10-14 36.114 37.0 37.0 37.0 37.0 37.0 15-19 36.067099999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.075900000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.0093 37.0 37.0 37.0 37.0 37.0 30-34 35.9717 37.0 37.0 37.0 37.0 37.0 35-39 35.8975 37.0 37.0 37.0 37.0 37.0 40-44 35.936 37.0 37.0 37.0 37.0 37.0 45-49 35.9533 37.0 37.0 37.0 37.0 37.0 50-54 35.85088434608652 37.0 37.0 37.0 37.0 37.0 55-59 35.85391347836959 37.0 37.0 37.0 37.0 37.0 60-64 35.74358589647412 37.0 37.0 37.0 37.0 37.0 65-69 35.74058514628657 37.0 37.0 37.0 37.0 37.0 70-74 35.73018254563641 37.0 37.0 37.0 37.0 37.0 75-79 35.72158179614939 37.0 37.0 37.0 37.0 37.0 80-84 35.54137068534267 37.0 37.0 37.0 37.0 37.0 85-89 35.65786020355687 37.0 37.0 37.0 37.0 37.0 90-94 35.53229922441831 37.0 37.0 37.0 37.0 37.0 95-99 35.501175881911436 37.0 37.0 37.0 37.0 37.0 100-104 35.438972572772926 37.0 37.0 37.0 37.0 37.0 105-109 35.34685604958137 37.0 37.0 37.0 34.6 37.0 110-114 35.386566018753825 37.0 37.0 37.0 37.0 37.0 115-119 35.29999925318244 37.0 37.0 37.0 32.2 37.0 120-124 35.23272760976565 37.0 37.0 37.0 29.8 37.0 125-129 35.198227756153116 37.0 37.0 37.0 32.2 37.0 130-134 35.15821010542057 37.0 37.0 37.0 27.4 37.0 135-139 35.17351202103825 37.0 37.0 37.0 25.0 37.0 140-144 34.95753457957611 37.0 37.0 37.0 25.0 37.0 145-149 34.9824675739835 37.0 37.0 37.0 25.0 37.0 150 34.79144233493908 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 0.0 13 2.0 14 3.0 15 0.0 16 1.0 17 0.0 18 0.0 19 2.0 20 2.0 21 7.0 22 14.0 23 5.0 24 8.0 25 10.0 26 6.0 27 14.0 28 13.0 29 29.0 30 38.0 31 58.0 32 83.0 33 128.0 34 279.0 35 668.0 36 2411.0 37 217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95 23.575 8.525 24.95 2 27.85 27.075 29.75 15.325 3 21.925 23.25 33.725 21.099999999999998 4 23.375 33.25 24.15 19.225 5 25.6 32.5 23.724999999999998 18.175 6 23.200000000000003 36.0 21.7 19.1 7 22.2 17.675 40.45 19.675 8 20.575 23.275000000000002 28.275 27.875 9 24.425 24.25 29.5 21.825 10-14 23.525 27.305 26.41 22.759999999999998 15-19 24.79 26.765 26.63 21.815 20-24 24.654999999999998 26.634999999999998 27.655 21.055 25-29 25.45 25.624999999999996 27.47 21.455 30-34 25.46 26.43 26.97 21.14 35-39 24.34 26.855 27.11 21.695 40-44 23.605 25.895000000000003 28.285 22.215 45-49 24.5 26.085 27.345000000000002 22.07 50-54 23.757375737573756 26.192619261926193 28.007800780078007 22.042204220422043 55-59 25.09127281820455 26.16154038509627 27.556889222305575 21.1902975743936 60-64 23.840960240060017 27.141785446361588 28.312078019504877 20.705176294073517 65-69 24.93623405851463 25.46636659164791 28.107026756689173 21.490372593148287 70-74 24.521130282570645 26.12653163290823 27.391847961990496 21.960490122530633 75-79 25.285114045618247 25.595238095238095 27.66106442577031 21.45858343337335 80-84 23.92696348174087 27.40370185092546 28.31415707853927 20.355177588794398 85-89 24.477133993795658 26.11828279795857 27.984589212448714 21.419993995797057 90-94 24.448336252189144 26.284713535151365 28.40630472854641 20.860645484113086 95-99 25.298974230673004 25.69427070302727 28.171128346259692 20.83562672004003 100-104 25.426612620727617 26.11719961967672 27.138067357253664 21.318120402341993 105-109 25.36834719855668 26.130099228224918 27.438107647589455 21.063445925628947 110-114 23.54185554548202 27.384909841845474 28.618918102145663 20.454316510526844 115-119 24.95308616929553 25.794999239235178 28.02150428564183 21.23041030582746 120-124 25.612380854770933 25.42789791944245 28.266885313108535 20.692835912678078 125-129 25.216759254452004 26.10456362597996 28.18649083640517 20.49218628316287 130-134 25.197722239797532 26.753137192871456 27.559843931245386 20.489296636085626 135-139 24.58551716761151 26.607123756551506 28.040432131778797 20.766926944058188 140-144 25.392156862745097 27.946623093681914 26.889978213507625 19.77124183006536 145-149 25.149232914923292 27.274755927475592 27.146443514644353 20.429567642956766 150 25.814678379144233 26.239727968262965 27.42986681779541 20.515726834797395 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 1.0 17 1.0 18 0.0 19 1.0 20 2.0 21 1.0 22 0.0 23 1.0 24 2.0 25 1.5 26 2.5 27 6.0 28 11.5 29 12.5 30 8.5 31 15.0 32 18.5 33 27.5 34 68.5 35 85.0 36 88.0 37 94.0 38 110.5 39 145.0 40 183.0 41 215.0 42 246.5 43 278.0 44 280.0 45 251.5 46 214.0 47 179.5 48 143.0 49 144.5 50 124.5 51 104.5 52 96.5 53 63.0 54 52.0 55 57.0 56 60.0 57 68.5 58 93.0 59 82.0 60 54.0 61 43.0 62 28.5 63 26.0 64 25.5 65 20.0 66 24.5 67 26.0 68 24.0 69 20.0 70 16.5 71 13.0 72 9.0 73 9.0 74 4.5 75 4.0 76 4.5 77 3.0 78 2.0 79 0.5 80 0.5 81 1.0 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 52-53 1.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 1.0 104-105 1.0 106-107 9.0 108-109 10.0 110-111 3.0 112-113 9.0 114-115 14.0 116-117 13.0 118-119 13.0 120-121 20.0 122-123 22.0 124-125 17.0 126-127 22.0 128-129 22.0 130-131 34.0 132-133 18.0 134-135 22.0 136-137 14.0 138-139 30.0 140-141 22.0 142-143 45.0 144-145 40.0 146-147 23.0 148-149 44.0 150-151 3529.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.849999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 54.71976401179941 27.825 2 21.878072763028516 22.25 3 11.209439528023598 17.1 4 5.555555555555555 11.3 5 3.048180924287119 7.75 6 1.4749262536873156 4.5 7 0.8849557522123894 3.15 8 0.4424778761061947 1.7999999999999998 9 0.24582104228121926 1.125 >10 0.5408062930186824 3.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAATTTCTCCAGCCGAAAAACTTCCTCCGAGGAAGAGCATCCTCTCGTC 18 0.44999999999999996 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 13 0.325 No Hit CTTGAATGTTTCCGAGTGCTGAAGTATGATGTGGAGTCAGATCCTCCGAG 12 0.3 No Hit CTTCCCTTTCTCTCATTTCATCCTACCTGATGAATCGATCCTAAAATATC 11 0.27499999999999997 No Hit TGGATGCGGATAGGTACGCTGCATTTAGAGAAATTGCTGGCGAATACCGC 11 0.27499999999999997 No Hit GCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAG 11 0.27499999999999997 No Hit ATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAA 11 0.27499999999999997 No Hit GACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTT 11 0.27499999999999997 No Hit CAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTGAC 10 0.25 No Hit ATCTACTCTAGTATGCGAGCTCCGTACTCCCGGGATCTGCAGTTTTTTGC 10 0.25 No Hit ATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAA 10 0.25 No Hit ATTGAAGTTTGCTGATGCAACACTTGCAAGATATGCTTTAAGTCGCCTAG 9 0.22499999999999998 No Hit CAGATCTAGGTCACACATGCTAAACATGGCCTACTTTAAAGACGATTCTA 9 0.22499999999999998 No Hit CATAAGAGACGCAGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAA 9 0.22499999999999998 No Hit CTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTCTCCCGGC 9 0.22499999999999998 No Hit GTTGAAGCGTCTCCTGCTGCACAAAATTTGACCCTGCGTGATCTGCCTCT 9 0.22499999999999998 No Hit CTCCACTCCACTCTCTCTCTCTCTGTTCGCCTCTCGCCTTTTTAGAGAGA 8 0.2 No Hit TGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTT 8 0.2 No Hit GTATGATGTGGAGTCAGATCCTCCGAGGACTCGGGAACTTGATACTGTTG 8 0.2 No Hit AATAAATCCCTGGTTGACAGAATGCGTGCTGCTTTAGGAATGGATGCGGA 8 0.2 No Hit CACATCTTGTCCCTGAAATGGCTAGGTTGTTACCTGATCCTTGGAAGCAG 8 0.2 No Hit GATTTCTAATGGCCACAGAAGGTGAAGTCATTAGCAACAGATTTGACACA 8 0.2 No Hit TAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTC 8 0.2 No Hit GGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCGGGGGA 8 0.2 No Hit CTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCCTTTCCAGATGCAT 8 0.2 No Hit GAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAG 7 0.17500000000000002 No Hit GTTTGAGAGTACCAATTGTGGTGGATTAGCTGAACCTCAACCGCAACATG 7 0.17500000000000002 No Hit GATGAAGCAAACCCTCCATACCCTTATCTTCCTACTGTCCCCGAGGAGCC 7 0.17500000000000002 No Hit AGGGGACATTGGTATTTTATCATTTAATTAGTAATATCGCTATCATCAGT 7 0.17500000000000002 No Hit AAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATTGACGCTGA 7 0.17500000000000002 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 7 0.17500000000000002 No Hit GTGTGACTGATCTTACACATGCGCTTCTGCAAGGAGAGAATGAGAATCAA 7 0.17500000000000002 No Hit GGATGATTGGACCCCTGTTTTGGAGCAAGGCAGGCCAGTCTTGTCTCAGT 7 0.17500000000000002 No Hit GTATATGCAAAAACTGGCTTTGCCGGTGGCATTAATTACTACCGCTGCCT 7 0.17500000000000002 No Hit GTTTGTACTTTTGAGCAGGAGTTGCTTTATTTAAATTATCATGCATTTCC 7 0.17500000000000002 No Hit CATCAGTCTTGAGAAAGGTTTCCATTGCATTCGTCCATTTGATATCTGCT 7 0.17500000000000002 No Hit AGGCTAGCCGCGCGCCTTTCCAGATGCATGCTCGATCGAGAGACGGCCGG 7 0.17500000000000002 No Hit GAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCA 7 0.17500000000000002 No Hit GTACTCTCCCGGCCGGCCGATCTACTCTAGTATGCGAGCTCCGTACTCCC 7 0.17500000000000002 No Hit GGAACAACTCCTAAGGAGATTGAGAAGGGCCATTCTTTCAGCAGAGAAAT 7 0.17500000000000002 No Hit AGAGACTTGAATGTTTCCGAGTGCTGAAGTATGATGTGGAGTCAGATCCT 7 0.17500000000000002 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 7 0.17500000000000002 No Hit CGGTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAG 7 0.17500000000000002 No Hit GTTCAGAGGTTTTTGCCTGTGCATGGAGCCCAGCTGGTTCTCTTCTAGCT 6 0.15 No Hit GAGAATTCGATGCCCAATTTAAGTGTGAGGAAAACAAGGAGATCATTTTC 6 0.15 No Hit CTCGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCCTTTCCAGAT 6 0.15 No Hit CTATGTAAATACATGTTCTGTCACTGTCATTCCGTTCTGTTCACAGTTAT 6 0.15 No Hit GTGGGGAGTTCTAGTGAAGTTGCTGGAAAAATGGAACAAGATGAATTCCC 6 0.15 No Hit GTTTTTATTGGGCTGTCATCCTGAGGAGAAATTGCCCCCATTTCCTCTGC 6 0.15 No Hit GTGGAGCAGTAGCTAGTACTCTCCCGGCCGGCCGATCTACTCTAGTATGC 6 0.15 No Hit CACAAGTTGTAATTTTGAGTGGGTGCGCCATTTGGAAGATGGCTCCGTAA 6 0.15 No Hit GGTGTATCTGTAGTTGCTGAGACCACAACAGGCTGTCTAATCTCTGCAGA 6 0.15 No Hit GTACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCC 6 0.15 No Hit GCCTCAACGAAAATGGCACTGGTGAAACCAGTACGTTGAAAGAGAACAAG 6 0.15 No Hit GCAATTTTGGATCGCATAAGAGACGCAGTGTTCAAGAAGGTTGCTGAGAA 6 0.15 No Hit GTTATAGCTAATCATTTTTTATCCAGTTTCCCGGCTCAAAAATAAATAGA 6 0.15 No Hit CTTTAAGTCGCCTAGTTGCTATGCGTTACCAAGCCAAACTAGGGATCATG 6 0.15 No Hit GGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGAC 6 0.15 No Hit CATATATTCACTGATCATAGATCTGGTTCATCTGGTGGAAGGTCAGCTGG 6 0.15 No Hit GTTCAATAAATATCTATCACAGGCTTCTTGGTATGTTATAGCTAATCATT 6 0.15 No Hit CATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAA 6 0.15 No Hit CCGGGCCATGGGACGGCAAAGATGGAGAGGTGATCGAAGAGGACGAGTTC 6 0.15 No Hit AGCTAATCATTTTTTATCCAGTTTCCCGGCTCAAAAATAAATAGATCAAC 6 0.15 No Hit GCTACATACACAAGGGCGGGCTCAAGAGAGACGTGCCCATGCTTGAGGAG 6 0.15 No Hit GAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCAT 6 0.15 No Hit TGTGAGGAAAACAAGGAGATCATTTTCTTTATGGATGTCATGCAATTCTT 6 0.15 No Hit GTTATTGCCACATCCACAAAGGTTGACATCTCTGGTGTTAAGGTGGAGAA 6 0.15 No Hit GCTAGGTTGTTACCTGATCCTTGGAAGCAGAAGGAACTTCTTGATGCCTA 6 0.15 No Hit GAATCAAGAAGGCTGGGATTGGAGGCCCCCTTATTAATTTTGACGGGGCT 6 0.15 No Hit GAATCATCCATAAAAGGAAAATGAACTGGCTTCTTTTTGGGTTATTTCTT 6 0.15 No Hit GTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTAT 6 0.15 No Hit GGACACTTGACATCCTTGGTAAGCAAATTCAAGAAAAACTCAATGCATGC 6 0.15 No Hit AGAAGAGAGACTTGAATGTTTCCGAGTGCTGAAGTATGATGTGGAGTCAG 6 0.15 No Hit GTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTTG 5 0.125 No Hit GGGAAATCTATATTTTCTGTACTAGGCTGGGATTGGAGGCCCCCTTATTA 5 0.125 No Hit CATACAGATCCAAGATTACCATCAAGCAGTGATACTGCAGTTATTATGTA 5 0.125 No Hit TGTAATGATTACGCATGGCAACATGGTGGCCACAACTGCTGCCGTCAGGA 5 0.125 No Hit GGCCGGGCTCGTATAACAAATCTGCAGTAGTGGAGCAGTAGCTAGTACTC 5 0.125 No Hit GAGAGGTGACAGATACAGTGGCAGGGATGATAGGTATGGTGGTGGTAGAT 5 0.125 No Hit GGCTGCTGGATGTTATGGCGTTCCACAATTTCGGATGCGTGTGTTTTTAT 5 0.125 No Hit GAAGATTCATGCTGGTGGTGATCCATTAATAATAGAGCTCCATTGCATGG 5 0.125 No Hit GGAGGAATGAGGGAAATGGAAAAATTGGAGGAACTATCAACGGTATGTCT 5 0.125 No Hit GGGAGAGAGCCTCGGGGGAAGGATCAAGAGAGGCTCGCCCGCGCGCGTGA 5 0.125 No Hit ACAGATTTGACACAGAATACTTGCTCGGATGTCGTTCTCGAGTACTTTCG 5 0.125 No Hit CGCTACTGTGAGCTATCCCAATATTGATGAAATGAGTAAAGAATCTGAGA 5 0.125 No Hit GTCGTCGATCTCGCTGCAAGCCTGCAAGCTATAGAGGCTAGCCGCGCGCC 5 0.125 No Hit CCTAAATTAAGTTATAGGTCGTGGGTTTTCATAGCTCGGATATGTGTGAC 5 0.125 No Hit TGCAAACACCCTTTTTTCTCTTCCTGAACCAAAGGTCCCGGCCGGTCGAT 5 0.125 No Hit GTACGCACTTATGCTTTATATTTAGAAGAGAGACTTGAATGTTTCCGAGT 5 0.125 No Hit AGTTGTAATTTTGAGTGGGTGCGCCATTTGGAAGATGGCTCCGTAAAATT 5 0.125 No Hit ATTTACTTCTGGCGAGATGTATATGGTATAAAGATGTCTTCTATGATGCC 5 0.125 No Hit AACTCCGGTCCATAAACTTGAGAATCCCACATGTAGCAGTATATACCTAA 5 0.125 No Hit GCTTTATATTTAGAAGAGAGACTTGAATGTTTCCGAGTGCTGAAGTATGA 5 0.125 No Hit AGAATCTGAGAAGCCTGAGCTAATGTCTCCGGAAGATCTTGGTGAACAAG 5 0.125 No Hit GTAGCTTACTGCATCCATGCCCTTGCAAGGCGTCTTTCCAAGACACGGAA 5 0.125 No Hit GCATGCTCGATCGAGAGACGGCCGGGCTCGTATAACAAATCTGCAGTAGT 5 0.125 No Hit GGGGCATTCATTGCAGCCATGGCAGTCTCTATGCTACTTCATGTCAGATC 5 0.125 No Hit GGTGAAGGGTTTAAGGGTCTTGTCTCAAGCGAAAACTTCCAAACATTGAA 5 0.125 No Hit GATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACT 5 0.125 No Hit GGAAAAGAACAACTTGCACCTTTTGTATGCAAAACGTACTTTGGATCCCG 5 0.125 No Hit CGGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGT 5 0.125 No Hit GGAAAACATAAATGATTGCCGAGAGCAGCAATTCGAGAGACTTTTAGGAT 5 0.125 No Hit CGGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTA 5 0.125 No Hit GGTGATGTCGATGTTGTATGTGGAGGTCCACCATGCCAAGGTATTAGTGG 5 0.125 No Hit TGTTGATGTGCAGAAGCTAATTGAGAAGGCCAAATCCCTACAGTCTATTG 5 0.125 No Hit GTTCTCGAGTACTTTCGTCCACTTGTAGAATCAAGAAGGCTGGGATTGGA 5 0.125 No Hit CTACATGGAAAACGTCCTTGATATATTGAAGTTTGCTGATGCAACACTTG 5 0.125 No Hit GTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTAT 5 0.125 No Hit GTCATCCTGAGGAGAAATTGCCCCCATTTCCTCTGCCTACACATGAAGCA 5 0.125 No Hit GACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGACCTTA 5 0.125 No Hit AGAGTATCTCCACAGATTGAAAACCGTATCCTCTATGCTGCACGTGGAAT 5 0.125 No Hit AGCAAGAGAGGGAAAAAGACAAAACTGAGAGAGACAAGGCGCAAGAGAAA 5 0.125 No Hit AGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCA 5 0.125 No Hit ATTTGGAAGATGGCTCCGTAAAATTCATAGAAGGAGCAAGGCAACCTACC 5 0.125 No Hit GGCAGGCCAGTCTTGTCTCAGTTTCCCAAGGGGCTGGGAGAGAGCCTCGG 5 0.125 No Hit CGGCGACCCAAGGAGTTCTCTATCTGACAAAAGAAATGTACATGTTCTCA 5 0.125 No Hit CCTTGGAAGCAGAAGGAACTTCTTGATGCCTATTACACCAATATGCGCTT 5 0.125 No Hit CTCTACTCAGCGAAACTTGCTTTGGTTGAAACTCGTAAGGTGGCTTTTGT 5 0.125 No Hit ACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCCAC 5 0.125 No Hit GCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGA 5 0.125 No Hit GGGCCATTCTTTCAGCAGAGAAATAAAGAAATCTTTGAGACAGAAGCTTG 5 0.125 No Hit CACAAGCCGGCGCCTCTGCTGATTGGAAATTTGGAGAGCAAATTGCTCAA 5 0.125 No Hit AGGTGGAGAAGTTTGATGACAAGTACTTTGCTAGGGACAAGAAGGCAAGG 5 0.125 No Hit GTCTTTGAATTTGAACAGACATGCAACTCCGGTCCATAAACTTGAGAATC 5 0.125 No Hit GCCAGTGCCCGTCGATGGCCAGATCCCGGCTCCTCTCGCCTTCTTCAACC 5 0.125 No Hit CTGGGATTGGAGGCCCCCTTATTAATTTTGACGGGGCTTTCGTTGGCATG 5 0.125 No Hit CTTATTAATTATGGCAGATCTAGGTCACACATGCTAAACATGGCCTACTT 5 0.125 No Hit TGTTACCTGATCCTTGGAAGCAGAAGGAACTTCTTGATGCCTATTACACC 5 0.125 No Hit ACAGATCCAAGATTACCATCAAGCAGTGATACTGCAGTTATTATGTATAC 5 0.125 No Hit CCTGATCCTTGGAAGCAGAAGGAACTTCTTGATGCCTATTACACCAATAT 5 0.125 No Hit GGCAGTCGGTGTGAAAAGTGATGCTCGAAATTCAGTAAGACGGTGCCTTC 5 0.125 No Hit GTGCTGATCCTGCTCGCCGGTAGGTACATGGGGAAGCGCGTGGTGTTCCT 5 0.125 No Hit GTGGTCACCAAGTGGGAAAACGTTGGCCTATGCAGGGCACAGTTCCATGA 5 0.125 No Hit GTCCAAGGGAAGTGTGAGGTCTTTTGCATGCACAGGCTTGCTTATAAAGT 5 0.125 No Hit AGTTGATCCCACATTTCGTGAAGAGCTTATTAATTATGGCAGATCTAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCACAG 10 0.0074129635 141.0875 1 GAACTTC 20 4.0002036E-4 105.81562 2 >>END_MODULE Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011408 spots for ERR5262794.sra Written 2011408 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra Read 2011397 spots for ERR5262794.sra Written 2011397 spots for ERR5262794.sra SRR ids: ['ERR5262794.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ucpmwibn ERR5262794.sra spots: 40227951 blocks: [[1, 2011397], [2011398, 4022794], [4022795, 6034191], [6034192, 8045588], [8045589, 10056985], [10056986, 12068382], [12068383, 14079779], [14079780, 16091176], [16091177, 18102573], [18102574, 20113970], [20113971, 22125367], [22125368, 24136764], [24136765, 26148161], [26148162, 28159558], [28159559, 30170955], [30170956, 32182352], [32182353, 34193749], [34193750, 36205146], [36205147, 38216543], [38216544, 40227951]] ERR5262794 file size 13186375 ERR5262794 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262794 ERR5262794_1.fastq ERR5262794_2.fastq Input file: ERR5262794_1.fastq Paired file: ERR5262794_2.fastq trimmed: ERR5262794-trimmed-pair1.fastq, ERR5262794-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:46:18 2024 >> started Fri Dec 6 11:47:06 2024 >> done (47.878s) 40227951 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 40227950 (100.00%) read pairs available; of these: 12110 ( 0.03%) trimmed read pairs available after processing 40215840 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 1 0.00% 21 0 0.00% 22 2 0.00% 23 1 0.00% 24 0 0.00% 25 1 0.00% 26 2 0.00% 27 2 0.00% 28 2 0.00% 29 1 0.00% 30 5 0.00% 31 3 0.00% 32 7 0.00% 33 4 0.00% 34 6 0.00% 35 5 0.00% 36 8 0.00% 37 4 0.00% 38 7 0.00% 39 12 0.00% 40 2 0.00% 41 1 0.00% 42 1 0.00% 43 4 0.00% 44 7 0.00% 45 1 0.00% 46 4 0.00% 47 7 0.00% 48 4 0.00% 49 374 0.00% 50 452 0.00% 51 522 0.00% 52 587 0.00% 53 558 0.00% 54 640 0.00% 55 683 0.00% 56 747 0.00% 57 870 0.00% 58 1036 0.00% 59 1128 0.00% 60 1411 0.00% 61 1559 0.00% 62 1741 0.00% 63 2035 0.01% 64 2030 0.01% 65 2347 0.01% 66 2690 0.01% 67 2780 0.01% 68 3288 0.01% 69 3644 0.01% 70 4333 0.01% 71 5117 0.01% 72 5617 0.01% 73 6591 0.02% 74 7432 0.02% 75 7878 0.02% 76 8719 0.02% 77 9796 0.02% 78 10537 0.03% 79 12130 0.03% 80 13587 0.03% 81 15054 0.04% 82 16757 0.04% 83 19281 0.05% 84 20614 0.05% 85 22915 0.06% 86 24704 0.06% 87 26403 0.07% 88 28151 0.07% 89 30252 0.08% 90 32582 0.08% 91 35082 0.09% 92 37746 0.09% 93 41100 0.10% 94 44559 0.11% 95 46139 0.11% 96 49576 0.12% 97 52184 0.13% 98 53912 0.13% 99 56556 0.14% 100 58551 0.15% 101 60669 0.15% 102 63992 0.16% 103 68181 0.17% 104 71322 0.18% 105 74675 0.19% 106 77993 0.19% 107 79599 0.20% 108 81566 0.20% 109 84466 0.21% 110 86145 0.21% 111 89010 0.22% 112 92058 0.23% 113 94427 0.23% 114 99146 0.25% 115 101628 0.25% 116 103886 0.26% 117 106302 0.26% 118 108062 0.27% 119 109870 0.27% 120 110547 0.27% 121 113543 0.28% 122 115435 0.29% 123 118528 0.29% 124 122907 0.31% 125 125571 0.31% 126 126562 0.31% 127 129361 0.32% 128 130466 0.32% 129 132784 0.33% 130 132230 0.33% 131 132963 0.33% 132 137339 0.34% 133 138280 0.34% 134 139646 0.35% 135 143496 0.36% 136 146388 0.36% 137 145761 0.36% 138 148280 0.37% 139 151309 0.38% 140 152776 0.38% 141 156827 0.39% 142 159584 0.40% 143 160810 0.40% 144 164504 0.41% 145 163211 0.41% 146 167698 0.42% 147 326501 0.81% 148 155487 0.39% 149 156534 0.39% 150 33334543 82.86% 40227950 reads passed initial QC criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=2.52 fanout-score-rank=37 prefix-density=0.22 prefix-fanout=2.3 sequence=CCCTTGAAACCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=318.33 fanout-score-rank=1 prefix-density=0.48 prefix-fanout=8.7 sequence=CCTTCTCCAGTCCAGCTCTTTCAAACTCGTTCAGTGGACCAAGTGGCAAGATCTCCTCAACGCCACATCGACCTAGGCGCACTTTGGATGCAAAGAAAGGCAGCTCCGTCACCTGAGAAGCTACATAAGAGCACTCTACTATCCCAGCATCACCATGCAATCCTCTTAAGCAAGCATCTGCGAATTTTGCTGCTGCATACGCCATAGAAAGAGTTGCTGATCCT criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=57.07 fanout-score-rank=3 prefix-density=0.82 prefix-fanout=12.4 sequence=CGCCGCCGCCGC criterion=fanout-score sequence-density=0.09 sequence-density-rank=20 fanout-score=190.24 fanout-score-rank=1 prefix-density=0.79 prefix-fanout=21.0 sequence=GCCGCCGCCGCAGCCGCCGACCGCAGCCATGTCTCTGATCGCGGGTGAGGAGTTCCAGCACATCCTGCGTCTGCTCAACACCAACGTCGATGGGAAGCAGAAGATCATGTTCGCGCTGACCTCCATCAAGGGTGTGGGCCGCCGCTTCTCCAACA ERR5262794 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:47:35 Started mapping on | Dec 06 11:47:35 Finished on | Dec 06 11:49:50 Mapping speed, Million of reads per hour | 1072.75 Number of input reads | 40227950 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 39106879 Uniquely mapped reads % | 97.21% Average mapped length | 290.56 Number of splices: Total | 36798459 Number of splices: Annotated (sjdb) | 34056053 Number of splices: GT/AG | 36269092 Number of splices: GC/AG | 464864 Number of splices: AT/AC | 26037 Number of splices: Non-canonical | 38466 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.00% Deletion average length | 1.51 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 395436 % of reads mapped to multiple loci | 0.98% Number of reads mapped to too many loci | 1097 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.79% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 725635 725635 725635 N_multimapping 395436 395436 395436 N_noFeature 1729174 37981752 2099246 N_ambiguous 871191 5608 115687 UnstrandedReadsAssigned:36506514 PositiveStrandReadsAssigned:1119519 NegativeStrandReadsAssigned:36891946 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262794 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262794-trimmed-pair1.fastq ERR5262794-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 40,227,950 reads, 37,426,184 reads pseudoaligned [quant] estimated average fragment length: 271.3 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,207 rounds 52973 ERR5262794.ke.tsv 35125 ERR5262794.se.tsv 88098 total ==> ERR5262794.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 666.534 0 0 PNS24247 1044 773.7 275.057 13.5373 PNS24249 1928 1657.7 599.187 13.7638 PNS24246 1044 773.7 275.057 13.5373 PNS24248 1044 773.7 275.057 13.5373 PNS24244 1471 1200.7 415.643 13.1816 PNS24243 293 101.896 0 0 KQK14069 1603 1332.7 83805.3 2394.53 KQK14071 474 236.748 300.287 48.2983 ==> ERR5262794.se.tsv <== BRADI_1g14170v3 84368 BRADI_1g53295v3 360 BRADI_1g59795v3 1401 BRADI_1g07683v3 0 BRADI_1g00485v3 56 BRADI_1g20270v3 1162 BRADI_1g74790v3 2606 BRADI_1g09890v3 0 BRADI_1g77505v3 489 BRADI_1g48960v3 0 ERR5262794 completed mapping pipeline successfully