Starting /dee2/code/volunteer_pipeline.sh ERR5262796 current disk space = 1551329189888 free memory = 1601454336 ERR5262796 SRAfilesize 8b2dd83869592ee1d738827ef9133bd3 ERR5262796.sra ERR5262796.sra file validated ERR5262796 is paired end ERR5262796 is conventional basespace ERR5262796 read1 length is 75-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262796_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 75-150 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6135 37.0 37.0 37.0 37.0 37.0 2 36.49325 37.0 37.0 37.0 37.0 37.0 3 36.585 37.0 37.0 37.0 37.0 37.0 4 36.604 37.0 37.0 37.0 37.0 37.0 5 36.769 37.0 37.0 37.0 37.0 37.0 6 36.723 37.0 37.0 37.0 37.0 37.0 7 36.543 37.0 37.0 37.0 37.0 37.0 8 36.554 37.0 37.0 37.0 37.0 37.0 9 36.651 37.0 37.0 37.0 37.0 37.0 10-14 36.5283 37.0 37.0 37.0 37.0 37.0 15-19 36.553399999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.575 37.0 37.0 37.0 37.0 37.0 25-29 36.594 37.0 37.0 37.0 37.0 37.0 30-34 36.5846 37.0 37.0 37.0 37.0 37.0 35-39 36.5564 37.0 37.0 37.0 37.0 37.0 40-44 36.5056 37.0 37.0 37.0 37.0 37.0 45-49 36.5219 37.0 37.0 37.0 37.0 37.0 50-54 36.3789 37.0 37.0 37.0 37.0 37.0 55-59 36.4499 37.0 37.0 37.0 37.0 37.0 60-64 36.4242 37.0 37.0 37.0 37.0 37.0 65-69 36.4073 37.0 37.0 37.0 37.0 37.0 70-74 36.3619 37.0 37.0 37.0 37.0 37.0 75-79 36.51087179715984 37.0 37.0 37.0 37.0 37.0 80-84 36.49078517676415 37.0 37.0 37.0 37.0 37.0 85-89 36.5046812259069 37.0 37.0 37.0 37.0 37.0 90-94 36.467759422628774 37.0 37.0 37.0 37.0 37.0 95-99 36.51550288173858 37.0 37.0 37.0 37.0 37.0 100-104 36.533200153184886 37.0 37.0 37.0 37.0 37.0 105-109 36.478414143221734 37.0 37.0 37.0 37.0 37.0 110-114 36.46345945307708 37.0 37.0 37.0 37.0 37.0 115-119 36.47178299369354 37.0 37.0 37.0 37.0 37.0 120-124 36.449872914187075 37.0 37.0 37.0 37.0 37.0 125-129 36.372649109136034 37.0 37.0 37.0 37.0 37.0 130-134 36.44105185102931 37.0 37.0 37.0 37.0 37.0 135-139 36.383720015312676 37.0 37.0 37.0 37.0 37.0 140-144 36.36028136244568 37.0 37.0 37.0 37.0 37.0 145-149 36.40509115674241 37.0 37.0 37.0 37.0 37.0 150 36.4827397260274 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 27 1.0 28 3.0 29 4.0 30 14.0 31 17.0 32 38.0 33 52.0 34 99.0 35 244.0 36 2946.0 37 582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.25 20.65 6.175 36.925000000000004 2 9.202300575143786 24.8062015503876 30.90772693173293 35.08377094273568 3 11.25 19.725 34.425 34.599999999999994 4 8.924999999999999 20.075000000000003 31.7 39.300000000000004 5 23.95 29.425 25.874999999999996 20.75 6 13.0 47.775 23.925 15.299999999999999 7 6.05 23.65 52.05 18.25 8 11.475 26.974999999999998 28.475 33.074999999999996 9 9.65 26.674999999999997 36.325 27.35 10-14 14.829999999999998 30.15 29.075 25.945 15-19 11.945 31.405 32.785 23.865 20-24 14.515 25.915 35.199999999999996 24.37 25-29 19.055 28.64 28.315 23.990000000000002 30-34 16.535 29.59 33.165 20.71 35-39 20.419999999999998 28.775000000000002 29.175 21.63 40-44 23.895 29.37 25.4 21.335 45-49 17.2 25.435000000000002 33.32 24.044999999999998 50-54 18.755 28.439999999999998 26.305 26.5 55-59 22.79 23.755000000000003 28.025 25.430000000000003 60-64 25.624999999999996 23.79 25.795 24.79 65-69 26.565 24.349999999999998 25.155 23.93 70-74 22.675 19.42 30.45 27.455000000000002 75-79 31.827279095366755 24.532172520764536 21.745221655158613 21.895326728710096 80-84 25.15021029441218 22.486481073502905 25.350490686961745 27.012817945123174 85-89 33.46020934541995 27.725747483347522 18.525567185856666 20.28847598537587 90-94 25.64513704464599 28.17557749160696 21.12541965225234 25.053865811494713 95-99 30.214708538175984 22.042741045449983 18.766930871877193 28.97561954449684 100-104 35.02484565577473 20.870350850775484 20.769964362796767 23.334839130653016 105-109 30.193418739010298 21.53227832202964 19.89449886963075 28.37980406932931 110-114 33.19391251763758 25.005039306591414 17.929852852247528 23.871195323523484 115-119 29.173616376042453 20.272934040940104 24.73591104371999 25.817538539297445 120-124 28.65989847715736 19.238578680203045 22.263959390862944 29.83756345177665 125-129 25.48849548492424 24.42222335595123 21.5091066782307 28.580174480893834 130-134 30.770419198681637 16.258111031002162 18.179009166752497 34.792460603563704 135-139 30.598415346121765 24.932235195996665 14.60070892410342 29.868640533778144 140-144 33.891412274215696 20.782718918347946 21.506284989965142 23.819583817471216 145-149 31.935047361299052 23.426251691474967 18.148849797023004 26.489851150202977 150 30.849315068493148 21.56164383561644 14.136986301369864 33.45205479452055 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 3.0 28 8.5 29 9.5 30 17.5 31 39.0 32 55.0 33 44.0 34 27.0 35 68.0 36 129.0 37 115.5 38 278.5 39 278.5 40 150.0 41 206.0 42 111.5 43 38.0 44 51.5 45 39.0 46 90.5 47 133.0 48 113.0 49 103.0 50 92.5 51 206.0 52 221.5 53 377.0 54 428.5 55 159.5 56 59.5 57 31.5 58 31.0 59 35.0 60 65.0 61 72.0 62 52.0 63 41.5 64 18.0 65 1.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 74-75 2.0 76-77 2.0 78-79 0.0 80-81 2.0 82-83 0.0 84-85 0.0 86-87 1.0 88-89 1.0 90-91 1.0 92-93 0.0 94-95 4.0 96-97 0.0 98-99 2.0 100-101 0.0 102-103 1.0 104-105 2.0 106-107 1.0 108-109 5.0 110-111 7.0 112-113 8.0 114-115 2.0 116-117 4.0 118-119 4.0 120-121 13.0 122-123 6.0 124-125 1.0 126-127 19.0 128-129 10.0 130-131 18.0 132-133 19.0 134-135 15.0 136-137 21.0 138-139 15.0 140-141 20.0 142-143 40.0 144-145 49.0 146-147 21.0 148-149 34.0 150-151 3650.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.35 #Duplication Level Percentage of deduplicated Percentage of total 1 33.03964757709251 3.75 2 18.502202643171806 4.2 3 7.488986784140969 2.55 4 8.370044052863436 3.8 5 5.286343612334802 3.0 6 2.8634361233480177 1.95 7 3.7444933920704844 2.9749999999999996 8 1.762114537444934 1.6 9 2.4229074889867843 2.475 >10 13.215859030837004 33.225 >50 2.4229074889867843 20.575 >100 0.881057268722467 19.900000000000002 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTCGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTA 397 9.925 No Hit AACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTAACGC 146 3.65 No Hit CAACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTAACG 140 3.5000000000000004 No Hit CACACATGACATGCCCATATGATTCATCATGAATTTAACGCTCGATCTGT 113 2.825 No Hit CTTTATTAACCAACCAAACACACACATGACATGCCCATATGATTCATCAT 98 2.45 No Hit CTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCTAGCC 95 2.375 No Hit ACATGACATGCCCATATGATTCATCATGAATTTAACGCTCGATCTGTTAT 94 2.35 No Hit GCCTTCTCCACATTTGTTGCATCCTTGGCACTAGTCTCAAGGAACGGTAT 86 2.15 No Hit CACATGACATGCCCATATGATTCATCATGAATTTAACGCTCGATCTGTTA 79 1.975 No Hit CTCGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAG 71 1.775 No Hit GATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCTA 62 1.55 No Hit ACTTTATTAACCAACCAAACACACACATGACATGCCCATATGATTCATCA 62 1.55 No Hit GATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGAT 60 1.5 No Hit CATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGA 58 1.4500000000000002 No Hit GTCATAAATGCCTTCTCCACATTTGTTGCATCCTTGGCACTAGTCTCAAG 58 1.4500000000000002 No Hit CTCTCTTTTGTTCCAGCTTTTTTATACATCATTAGTTTACGGCGGCATTC 50 1.25 No Hit ACACACATGACATGCCCATATGATTCATCATGAATTTAACGCTCGATCTG 47 1.175 No Hit CCACATTTGTTGCATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAATC 45 1.125 No Hit AACCAACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTA 45 1.125 No Hit CGGTATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTCGTAAGAAACTA 44 1.0999999999999999 No Hit ACACATGACATGCCCATATGATTCATCATGAATTTAACGCTCGATCTGTT 42 1.05 No Hit ATATGATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTG 37 0.9249999999999999 No Hit CGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCT 37 0.9249999999999999 No Hit CTCCACATTTGTTGCATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAA 37 0.9249999999999999 No Hit GCTCCTCCTTGGATGCTAGTTCCAAGATATGTAGGGTTGACACACTGTGC 34 0.8500000000000001 No Hit CGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCT 33 0.8250000000000001 No Hit ATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGAT 32 0.8 No Hit ACCAACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTAA 32 0.8 No Hit CACATTTGTTGCATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAATCT 32 0.8 No Hit CCGAAGTTTAGGGATAAACTTATTCTTTGTTTAAGAAAGAAATAAAAAGT 30 0.75 No Hit GTTGCATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGC 30 0.75 No Hit ATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCTAG 29 0.7250000000000001 No Hit GTCAGGCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATTCAGAATCCACT 29 0.7250000000000001 No Hit GTCAGACACACGCGCGGACGGTGTCAAAATCCACAAGCTGATCGTCGAAC 29 0.7250000000000001 No Hit CTTTTGTTCCAGCTTTTTTATACATCATTAGTTTACGGCGGCATTCTTGT 28 0.7000000000000001 No Hit CTCTTTTGTTCCAGCTTTTTTATACATCATTAGTTTACGGCGGCATTCTT 27 0.675 No Hit CCTCGATGGCCAACATCACCTTGTCCACGGTCACCTTGTTCCCGAGGCTG 26 0.65 No Hit GTCTCAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTC 26 0.65 No Hit GCCAACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTAA 25 0.625 No Hit GTCGGCTTCCGCCAGCACGTGCCCCTCGATGGCCAACATCACCTTGTCCA 22 0.5499999999999999 No Hit CTTCTCCACATTTGTTGCATCCTTGGCACTAGTCTCAAGGAACGGTATTC 22 0.5499999999999999 No Hit GTATGATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTG 21 0.525 No Hit GCTCGGTCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTA 18 0.44999999999999996 No Hit GCACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCTTGCC 18 0.44999999999999996 No Hit GTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCTAGCCAG 18 0.44999999999999996 No Hit GCACTTTATTAACCAACCAAACACACACATGACATGCCCATATGATTCAT 17 0.42500000000000004 No Hit TCCACATTTGTTGCATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAAT 17 0.42500000000000004 No Hit CTTTGTTTAAGAAAGAAATAAAAAGTTCTCTCTTTTGTTCCAGCTTTTTT 17 0.42500000000000004 No Hit ACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTAACGCT 17 0.42500000000000004 No Hit ATGATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAG 16 0.4 No Hit TATGATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGA 16 0.4 No Hit CCCTCGATGGCCAACATCACCTTGTCCACGGTCACCTTGTTCCCGAGGCT 15 0.375 No Hit TGATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGA 14 0.35000000000000003 No Hit CCAACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTAAC 14 0.35000000000000003 No Hit CTCCTCCTTGGATGCTAGTTCCAAGATATGTAGGGTTGACACACTGTGCT 13 0.325 No Hit GGCACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCTTGC 13 0.325 No Hit GCGCGGACGGTGTCAAAATCCACAAGCTGATCGTCGAACACTTATCCAGA 13 0.325 No Hit ATTTAACGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAA 13 0.325 No Hit TCGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGC 12 0.3 No Hit ATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGATC 12 0.3 No Hit GAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGATGG 12 0.3 No Hit CTCCTCTCCAGTGTGTCCACCAAGAACCAAGACCCTGGTGCCACCAACCA 12 0.3 No Hit ATCCACTCCTCTCCAGTGTGTCCACCAAGAACCAAGACCCTGGTGCCACC 12 0.3 No Hit CACTTTATTAACCAACCAAACACACACATGACATGCCCATATGATTCATC 12 0.3 No Hit GCTTTATGTAACGGTATTGTAGACTTGCGGATTGATCTGATCTCCAGTCT 11 0.27499999999999997 No Hit GGCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATTCAGAATCCACTCCTC 11 0.27499999999999997 No Hit CACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCTTGCCA 11 0.27499999999999997 No Hit GGGATAAACTTATTCTTTGTTTAAGAAAGAAATAAAAAGTTCTCTCTTTT 11 0.27499999999999997 No Hit GGATAAACTTATTCTTTGTTTAAGAAAGAAATAAAAAGTTCTCTCTTTTG 11 0.27499999999999997 No Hit ACGGTATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTCGTAAGAAACT 11 0.27499999999999997 No Hit CTCAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTCGT 11 0.27499999999999997 No Hit TATTAACCAACCAAACACACACATGACATGCCCATATGATTCATCATGAA 10 0.25 No Hit GCGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGC 10 0.25 No Hit TTTTTTTTTCAGACTTAATTATAGGTGTATATTGCACATAACTTCAATTC 10 0.25 No Hit TTTATTAACCAACCAAACACACACATGACATGCCCATATGATTCATCATG 10 0.25 No Hit GTTTAAGAAAGAAATAAAAAGTTCTCTCTTTTGTTCCAGCTTTTTTATAC 9 0.22499999999999998 No Hit GGTTGACACACTGTGCTGGTAAAGTTCAACTGCCAGACTCATGTCTTGCT 9 0.22499999999999998 No Hit GCTTGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTA 9 0.22499999999999998 No Hit GTCAAAATCCACAAGCTGATCGTCGAACACTTATCCAGAGAACACGGCCG 9 0.22499999999999998 No Hit CCGTGCTGCAATTCCTGAGCACAGGACAAAAACATATTGTACCATGCACT 9 0.22499999999999998 No Hit CATAAATGCCTTCTCCACATTTGTTGCATCCTTGGCACTAGTCTCAAGGA 9 0.22499999999999998 No Hit GCACATGACATGCCCATATGATTCATCATGAATTTAACGCTCGATCTGTT 9 0.22499999999999998 No Hit ATTTGTTGCATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAATCTCAT 9 0.22499999999999998 No Hit CTATGATTCATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTG 9 0.22499999999999998 No Hit CAGGCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATTCAGAATCCACTCC 9 0.22499999999999998 No Hit CGTCAGGCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATTCAGAATCCAC 9 0.22499999999999998 No Hit ATCATGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGATCCATG 8 0.2 No Hit GGCCAACATCACCTTGTCCACGGTCACCTTGTTCCCGAGGCTGAGCACGT 8 0.2 No Hit CGGTATTGTAGACTTGCGGATTGATCTGATCTCCAGTCTTTAAATATTTG 8 0.2 No Hit GGCTTCCGCCAGCACGTGCCCCTCGATGGCCAACATCACCTTGTCCACGG 8 0.2 No Hit ATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTCGTAAGAAACTACCCT 8 0.2 No Hit CATCGCTCCTCCTTGGATGCTAGTTCCAAGATATGTAGGGTTGACACACT 8 0.2 No Hit CAGGAATCTTCGTCAGGCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATT 8 0.2 No Hit GCACACATGACATGCCCATATGATTCATCATGAATTTAACGCTCGATCTG 8 0.2 No Hit GGTCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCTA 7 0.17500000000000002 No Hit GACCAACCAAACACACACATGACATGCCCATATGATTCATCATGAATTTA 7 0.17500000000000002 No Hit TCGGTCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGC 7 0.17500000000000002 No Hit GCTAGTTCCAAGATATGTAGGGTTGACACACTGTGCTGGTAAAGTTCAAC 7 0.17500000000000002 No Hit TGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCTAGCCA 7 0.17500000000000002 No Hit GTTCTCTCTTTTGTTCCAGCTTTTTTATACATCATTAGTTTACGGCGGCA 7 0.17500000000000002 No Hit AATCCGCTTTATGTAACGGTATTGTAGACTTGCGGATTGATCTGATCTCC 7 0.17500000000000002 No Hit GGTCAGACACACGCGCGGACGGTGTCAAAATCCACAAGCTGATCGTCGAA 7 0.17500000000000002 No Hit CTTCGTCAGGCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATTCAGAATC 7 0.17500000000000002 No Hit GGTCGGCTTCCGCCAGCACGTGCCCCTCGATGGCCAACATCACCTTGTCC 7 0.17500000000000002 No Hit GAACGGTATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTCGTAAGAAA 7 0.17500000000000002 No Hit TGAATTTAACGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGATG 7 0.17500000000000002 No Hit CGCCAGCACGTGCCCCTCGATGGCCAACATCACCTTGTCCACGGTCACCT 7 0.17500000000000002 No Hit GCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATTCAGAATCCACTCCTCT 7 0.17500000000000002 No Hit GCTTTATTAACCAACCAAACACACACATGACATGCCCATATGATTCATCA 7 0.17500000000000002 No Hit GGTCATAAATGCCTTCTCCACATTTGTTGCATCCTTGGCACTAGTCTCAA 7 0.17500000000000002 No Hit GCATTATATTACTGAATTGAGATGTTTCATTCAGAGGCCAGGAGAGTTTC 7 0.17500000000000002 No Hit ATGAGAAACAACTGCATTGATTTTTTTGCAACTTTTCGGCAACGGGAGAG 6 0.15 No Hit GACGGTGTCAAAATCCACAAGCTGATCGTCGAACACTTATCCAGAGAACA 6 0.15 No Hit GCATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGCAAG 6 0.15 No Hit GTCCCTCGATGGCCAACATCACCTTGTCCACGGTCACCTTGTTCCCGAGG 6 0.15 No Hit GTTTCATTCAGAGGCCAGGAGAGTTTCTCTCCCGTTGCCGAAAAGTTGCA 6 0.15 No Hit CTCCAGTGTGTCCACCAAGAACCAAGACCCTGGTGCCACCAACCACACAG 6 0.15 No Hit TTTTGTTCCAGCTTTTTTATACATCATTAGTTTACGGCGGCATTCTTGTA 6 0.15 No Hit GCGGACGGTGTCAAAATCCACAAGCTGATCGTCGAACACTTATCCAGAGA 6 0.15 No Hit GCCAGCACGTGCCCCTCGATGGCCAACATCACCTTGTCCACGGTCACCTT 6 0.15 No Hit CGATGGCCAACATCACCTTGTCCACGGTCACCTTGTTCCCGAGGCTGAGC 6 0.15 No Hit GATAAACTTATTCTTTGTTTAAGAAAGAAATAAAAAGTTCTCTCTTTTGT 6 0.15 No Hit GTAGGGTTGACACACTGTGCTGGTAAAGTTCAACTGCCAGACTCATGTCT 6 0.15 No Hit AGGCCTGCTTGCAAGGCAAAGCTCATGGAGCTCATTCAGAATCCACTCCT 6 0.15 No Hit CCGCCAGCACGTGCCCCTCGATGGCCAACATCACCTTGTCCACGGTCACC 5 0.125 No Hit CTTGGCACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCT 5 0.125 No Hit CACTGACATAATCACATCGCTCCTCCTTGGATGCTAGTTCCAAGATATGT 5 0.125 No Hit CCTCTCCAGTGTGTCCACCAAGAACCAAGACCCTGGTGCCACCAACCACA 5 0.125 No Hit ACTCCTCTCCAGTGTGTCCACCAAGAACCAAGACCCTGGTGCCACCAACC 5 0.125 No Hit CAAACAAAGAGAAACACTTTATTAACCAACCAAACACACACATGACATGC 5 0.125 No Hit GTATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTCGTAAGAAACTACC 5 0.125 No Hit TAACCAACCAAACACACACATGACATGCCCATATGATTCATCATGAATTT 5 0.125 No Hit TTTTTTTTTTTTTTTGAGAATGTGCAATCCGCTTTATGTAACGGTATTGT 5 0.125 No Hit AAACAAAGAGAAACACTTTATTAACCAACCAAACACACACATGACATGCC 5 0.125 No Hit GTTGACACACTGTGCTGGTAAAGTTCAACTGCCAGACTCATGTCTTGCTG 5 0.125 No Hit GTGCAATCCGCTTTATGTAACGGTATTGTAGACTTGCGGATTGATCTGAT 5 0.125 No Hit GTCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGCTAGCTAG 5 0.125 No Hit ATCCTTGGCACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGCAAGGG 5 0.125 No Hit GCTCCTCTCCAGTGTGTCCACCAAGAACCAAGACCCTGGTGCCACCAACC 5 0.125 No Hit CTCCAATTGGCATGCATTATATTACTGAATTGAGATGTTTCATTCAGAGG 5 0.125 No Hit CAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCTTGCCAGCCTCGTAA 5 0.125 No Hit CAGCCAAACACACACATGACATGCCCATATGATTCATCATGAATTTAACG 5 0.125 No Hit GCACTGACATAATCACATCGCTCCTCCTTGGATGCTAGTTCCAAGATATG 5 0.125 No Hit CCGCTCGATCTGTTATACATTTATTCTTGAGATCCATGGATGGAACATGC 5 0.125 No Hit CCACTCCTCTCCAGTGTGTCCACCAAGAACCAAGACCCTGGTGCCACCAA 5 0.125 No Hit GTACCATGCACTGACATAATCACATCGCTCCTCCTTGGATGCTAGTTCCA 5 0.125 No Hit ACTAGTCTCAAGGAACGGTATTCCAATCTCATCAGCAAGGGCCTTGCCAG 5 0.125 No Hit ATGCACTGACATAATCACATCGCTCCTCCTTGGATGCTAGTTCCAAGATA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAATGC 10 0.0071935453 142.5125 5 GTCATAA 10 0.0071935453 142.5125 1 TGCCTTC 10 0.0071935453 142.5125 9 ATAAATG 10 0.0071935453 142.5125 4 ATGCCTT 10 0.0071935453 142.5125 8 AATGCCT 10 0.0071935453 142.5125 7 TCATAAA 10 0.0071935453 142.5125 2 AAATGCC 10 0.0071935453 142.5125 6 CATAAAT 10 0.0071935453 142.5125 3 ACGATGT 20 0.004828328 30.241379 140-144 GCTCGAT 190 5.8438836E-6 26.252302 1 TCTGTTA 220 5.638394E-7 25.911364 7 CGACGAT 30 0.0011283702 25.20115 140-144 TCGATCT 200 8.710347E-6 24.939688 3 CTCGATC 200 8.710347E-6 24.939688 2 TGTTATA 230 8.318184E-7 24.784782 9 CTGTTAT 230 8.318184E-7 24.784782 8 CGATCTG 205 1.0553284E-5 24.331402 4 GATCTGT 210 1.2725433E-5 23.752083 5 ATCTGTT 215 1.5275207E-5 23.199709 6 >>END_MODULE ERR5262796 read2 length is 75-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262796_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 75-150 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.236 37.0 37.0 37.0 37.0 37.0 2 35.9935 37.0 37.0 37.0 37.0 37.0 3 35.9185 37.0 37.0 37.0 37.0 37.0 4 36.0795 37.0 37.0 37.0 37.0 37.0 5 36.252 37.0 37.0 37.0 37.0 37.0 6 36.15 37.0 37.0 37.0 37.0 37.0 7 36.0375 37.0 37.0 37.0 37.0 37.0 8 36.1635 37.0 37.0 37.0 37.0 37.0 9 36.107 37.0 37.0 37.0 37.0 37.0 10-14 36.170399999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.109899999999996 37.0 37.0 37.0 37.0 37.0 20-24 35.9665 37.0 37.0 37.0 37.0 37.0 25-29 35.8902 37.0 37.0 37.0 37.0 37.0 30-34 35.954899999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.029 37.0 37.0 37.0 37.0 37.0 40-44 36.0544 37.0 37.0 37.0 37.0 37.0 45-49 35.9405 37.0 37.0 37.0 37.0 37.0 50-54 35.757999999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.84060000000001 37.0 37.0 37.0 37.0 37.0 60-64 35.9612 37.0 37.0 37.0 37.0 37.0 65-69 36.044599999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.9803 37.0 37.0 37.0 37.0 37.0 75-79 36.024713911610455 37.0 37.0 37.0 37.0 37.0 80-84 35.964698460102426 37.0 37.0 37.0 37.0 37.0 85-89 35.98928056463541 37.0 37.0 37.0 37.0 37.0 90-94 35.904949462944934 37.0 37.0 37.0 37.0 37.0 95-99 35.95404366580991 37.0 37.0 37.0 37.0 37.0 100-104 35.960187500314944 37.0 37.0 37.0 37.0 37.0 105-109 35.91502966370715 37.0 37.0 37.0 37.0 37.0 110-114 35.85017729776395 37.0 37.0 37.0 37.0 37.0 115-119 35.855800296138625 37.0 37.0 37.0 37.0 37.0 120-124 35.823081188771006 37.0 37.0 37.0 37.0 37.0 125-129 35.82988139429493 37.0 37.0 37.0 37.0 37.0 130-134 35.814266310290286 37.0 37.0 37.0 37.0 37.0 135-139 35.707252623569744 37.0 37.0 37.0 37.0 37.0 140-144 35.657050418461345 37.0 37.0 37.0 37.0 37.0 145-149 35.67022724683771 37.0 37.0 37.0 37.0 37.0 150 35.67108533554267 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 2.0 13 4.0 14 6.0 15 0.0 16 4.0 17 0.0 18 3.0 19 2.0 20 2.0 21 7.0 22 6.0 23 6.0 24 8.0 25 4.0 26 9.0 27 6.0 28 10.0 29 8.0 30 17.0 31 30.0 32 52.0 33 89.0 34 147.0 35 509.0 36 2795.0 37 269.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.175000000000004 17.299999999999997 6.8500000000000005 33.675 2 27.500000000000004 23.799999999999997 22.125 26.575 3 24.349999999999998 26.424999999999997 23.5 25.724999999999998 4 23.45 32.175 16.5 27.875 5 28.825 31.874999999999996 16.025 23.275000000000002 6 22.975 30.725 17.8 28.499999999999996 7 22.75 24.525 25.424999999999997 27.3 8 22.175 24.5 18.6 34.725 9 23.549999999999997 28.349999999999998 19.975 28.125 10-14 24.645 26.314999999999998 19.59 29.45 15-19 25.805 25.465 20.47 28.26 20-24 26.665 26.47 24.099999999999998 22.765 25-29 26.369999999999997 25.96 25.83 21.84 30-34 25.5 24.635 25.4 24.465 35-39 27.075 24.044999999999998 24.04 24.84 40-44 26.405 24.705 23.580000000000002 25.31 45-49 26.685 23.544999999999998 23.77 26.0 50-54 28.455000000000002 25.240000000000002 24.41 21.895 55-59 30.11 21.855 23.9 24.135 60-64 29.794999999999998 20.925 22.74 26.540000000000003 65-69 30.404999999999998 24.2 20.41 24.985 70-74 27.415 24.845 21.465 26.275 75-79 27.654358050635448 21.1848293805664 24.2119483638547 26.94886420494346 80-84 26.32844192918315 20.64907096709571 22.572244202934844 30.450242900786296 85-89 29.272690843518333 20.977759967942298 20.4217591664997 29.327790022039675 90-94 31.18705216214862 21.60645387583304 20.634363882347046 26.57213007967129 95-99 30.66619845490117 25.07775659676934 20.597973311929366 23.65807163640012 100-104 31.405912764141945 23.164182101089192 20.5089594940521 24.92094564071676 105-109 31.90153227832203 21.316252197940216 21.26098970108013 25.521225822657623 110-114 30.80024188671639 22.46522878451925 21.099576698246324 25.634952630518043 115-119 33.18675764468031 20.763204447814 21.75890826383624 24.291129643669446 120-124 29.796954314720814 20.736040609137056 23.487309644670052 25.979695431472084 125-129 28.42712106525177 21.784602826386408 23.549818886791492 26.23845722157033 130-134 27.55990724040196 26.42617881989178 20.659623808296832 25.354290131409428 135-139 27.599352446602953 27.510574964750116 19.84960050133166 25.040472087315262 140-144 29.463528413910094 29.12425784563189 19.290712468193387 22.12150127226463 145-149 27.001527050610818 28.086823734729492 21.93499127399651 22.976657940663177 150 24.60646230323115 29.63269814968241 21.458160729080365 24.302678818006076 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 0.5 22 1.5 23 2.0 24 1.0 25 1.5 26 1.5 27 1.5 28 1.5 29 1.5 30 2.0 31 2.5 32 3.5 33 7.0 34 10.0 35 10.5 36 10.5 37 21.0 38 36.5 39 51.0 40 47.5 41 50.0 42 58.5 43 71.0 44 76.0 45 86.0 46 126.5 47 99.0 48 199.5 49 439.5 50 370.0 51 158.0 52 93.0 53 83.5 54 74.5 55 60.5 56 65.0 57 65.0 58 74.5 59 144.5 60 184.5 61 204.5 62 234.0 63 180.5 64 99.5 65 49.5 66 80.0 67 115.5 68 63.0 69 16.5 70 13.5 71 36.0 72 46.5 73 26.5 74 20.0 75 11.5 76 2.5 77 1.0 78 0.5 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 74-75 2.0 76-77 2.0 78-79 1.0 80-81 2.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.0 90-91 1.0 92-93 0.0 94-95 4.0 96-97 0.0 98-99 2.0 100-101 0.0 102-103 1.0 104-105 2.0 106-107 1.0 108-109 5.0 110-111 7.0 112-113 8.0 114-115 2.0 116-117 4.0 118-119 4.0 120-121 13.0 122-123 6.0 124-125 1.0 126-127 19.0 128-129 11.0 130-131 19.0 132-133 22.0 134-135 16.0 136-137 23.0 138-139 17.0 140-141 23.0 142-143 47.0 144-145 58.0 146-147 21.0 148-149 34.0 150-151 3621.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.0 #Duplication Level Percentage of deduplicated Percentage of total 1 49.53703703703704 13.375 2 14.722222222222223 7.95 3 11.38888888888889 9.225 4 6.018518518518518 6.5 5 4.074074074074074 5.5 6 2.314814814814815 3.75 7 1.9444444444444444 3.675 8 1.4814814814814816 3.2 9 1.1111111111111112 2.7 >10 6.944444444444445 33.4 >50 0.3703703703703704 7.000000000000001 >100 0.0925925925925926 3.7249999999999996 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACG 149 3.7249999999999996 No Hit CCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTA 88 2.1999999999999997 No Hit CAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTT 80 2.0 No Hit GCACTCAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCC 61 1.525 No Hit GCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAA 51 1.275 No Hit GTGACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAA 46 1.15 No Hit CGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGACTTCCTGCCCAACC 42 1.05 No Hit CAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAG 40 1.0 No Hit CGATGACTTCCTGCCCAACCGCGTCCGCATCTTCGTCGACACCGTCGCCG 33 0.8250000000000001 No Hit GCGAGGTGACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAG 32 0.8 No Hit CACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTTT 32 0.8 No Hit ACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATT 31 0.775 No Hit AGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTA 29 0.7250000000000001 No Hit GGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAAGC 28 0.7000000000000001 No Hit ATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGGTGGT 27 0.675 No Hit GGCAAAGAAGGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCC 27 0.675 No Hit AGAAGGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCC 26 0.65 No Hit CGTTAATTAGTTTGGCCGGGACGATATGAGTTCCTCCTCCGTCGGCGAGG 25 0.625 No Hit GCCTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATG 24 0.6 No Hit GGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAAGCCTGA 24 0.6 No Hit GTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAAGCC 23 0.575 No Hit CCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTTTGG 22 0.5499999999999999 No Hit GTTAATTAGTTTGGCCGGGACGATATGAGTTCCTCCTCCGTCGGCGAGGT 21 0.525 No Hit AACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGT 21 0.525 No Hit GGTGACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAA 20 0.5 No Hit GCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCA 19 0.475 No Hit CTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAA 19 0.475 No Hit GCAAGATGGAAAGACAATAAAGCTGCAAATTTGGGACACTGCTGGCCAAG 19 0.475 No Hit GATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGGT 19 0.475 No Hit TATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGGTGG 19 0.475 No Hit GCTTCAACAACGTCAAGCAGTGGCTGAATGAAATTGACAGGTATGCCAGT 19 0.475 No Hit GTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGA 18 0.44999999999999996 No Hit CATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAAGCCTGACGCCGACA 18 0.44999999999999996 No Hit GGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTA 18 0.44999999999999996 No Hit GGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACA 17 0.42500000000000004 No Hit GCATCTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAGCA 17 0.42500000000000004 No Hit CCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGACTTCCTG 16 0.4 No Hit GGGGCAACGCCCGCGGCGATGACGCCGTCCAGGAAGGGATCAACGACTGG 16 0.4 No Hit GGACGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGG 16 0.4 No Hit GACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGA 16 0.4 No Hit ACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTTTG 16 0.4 No Hit GAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGG 16 0.4 No Hit CGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTTTGGCCGGG 16 0.4 No Hit AGGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTC 16 0.4 No Hit CGGGACGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCC 15 0.375 No Hit AGCACTCAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATC 15 0.375 No Hit GGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCG 15 0.375 No Hit GAGAGCTTCAACAACGTCAAGCAGTGGCTGAATGAAATTGACAGGTATGC 15 0.375 No Hit TGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGGG 14 0.35000000000000003 No Hit GGCGAAGGTTACCGATGACTTCCTGCCCAACCGCGTCCGCATCTTCGTCG 14 0.35000000000000003 No Hit GTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAA 13 0.325 No Hit GTTGATTTTAAAATCCGCACCGTTGAGCAAGATGGAAAGACAATAAAGCT 13 0.325 No Hit GAGCGATTTAGGACCATCACAAGCAGCTACTACCGTGGTGCCCATGGCAT 13 0.325 No Hit GTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGG 13 0.325 No Hit CCTGCCCGTCGGGGCGAAGGTTACCGATGACTTCCTGCCCAACCGCGTCC 13 0.325 No Hit ATTATTATCAGCTGATTGATCCACGTTAATTAGTTTGGCCGGGACGATAT 13 0.325 No Hit GTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGGTGGTCGGG 13 0.325 No Hit CCTGCCCAACCGCGTCCGCATCTTCGTCGACACCGTCGCCGAGATCCCCC 12 0.3 No Hit CACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAAT 12 0.3 No Hit GATGACTTCCTGCCCAACCGCGTCCGCATCTTCGTCGACACCGTCGCCGA 12 0.3 No Hit GGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAAGCCTGAC 12 0.3 No Hit GCCAGTGAAAATGTGAACAAGCTTTTGGTTGGGAACAAATGCGACCTGGC 12 0.3 No Hit CATCATTGTAGTCTATGATGTGACTGACCAGGAGAGCTTCAACAACGTCA 12 0.3 No Hit AGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGAC 12 0.3 No Hit CCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGA 12 0.3 No Hit ACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAA 12 0.3 No Hit CGGGGCGAAGGTTACCGATGACTTCCTGCCCAACCGCGTCCGCATCTTCG 12 0.3 No Hit GTCGGGGCGAAGGTTACCGATGACTTCCTGCCCAACCGCGTCCGCATCTT 11 0.27499999999999997 No Hit GACGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGA 11 0.27499999999999997 No Hit ACCGCGTCCGCATCTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGC 11 0.27499999999999997 No Hit CCAGCACTCAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGA 11 0.27499999999999997 No Hit GCGGAATCAGCTTGAGTGAAGAGACTGATGTTTGCAAAGATTTCCCTCGT 10 0.25 No Hit GCATCATTGTAGTCTATGATGTGACTGACCAGGAGAGCTTCAACAACGTC 10 0.25 No Hit GACCAGGAGAGCTTCAACAACGTCAAGCAGTGGCTGAATGAAATTGACAG 10 0.25 No Hit CGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGACTTCC 10 0.25 No Hit CCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAA 10 0.25 No Hit GAGGTGACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGC 10 0.25 No Hit CCTACACAATGGATGTTGGTGAAAAGAACCCACAATGGAGGCAGTTGGCG 10 0.25 No Hit GATCGGGTTCGCGGAATCAGCTTGAGTGAAGAGACTGATGTTTGCAAAGA 10 0.25 No Hit ACTCAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCAC 10 0.25 No Hit GCCCGTCGGGGCGAAGGTTACCGATGACTTCCTGCCCAACCGCGTCCGCA 9 0.22499999999999998 No Hit CAGCACTCAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGAT 9 0.22499999999999998 No Hit GAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGGTGGTCG 9 0.22499999999999998 No Hit CACTCAGCACCAACACCAAGCGCTAATATTATTATCAGCTGATTGATCCA 9 0.22499999999999998 No Hit GGACACTGCTGGCCAAGAGCGATTTAGGACCATCACAAGCAGCTACTACC 9 0.22499999999999998 No Hit GGGACGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCG 9 0.22499999999999998 No Hit GCGAAGGTTACCGATGACTTCCTGCCCAACCGCGTCCGCATCTTCGTCGA 9 0.22499999999999998 No Hit GCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAAGCCTGACG 9 0.22499999999999998 No Hit GTTACCGATGACTTCCTGCCCAACCGCGTCCGCATCTTCGTCGACACCGT 9 0.22499999999999998 No Hit GTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAGCATGTTCCAT 9 0.22499999999999998 No Hit GAAATTGACAGGTATGCCAGTGAAAATGTGAACAAGCTTTTGGTTGGGAA 9 0.22499999999999998 No Hit CAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGG 9 0.22499999999999998 No Hit TATCAGCTGATTGATCCACGTTAATTAGTTTGGCCGGGACGATATGAGTT 8 0.2 No Hit GTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGACTTCCTGCCCAACCG 8 0.2 No Hit CGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAG 8 0.2 No Hit CCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAGCATGTTCCATCCATGGA 8 0.2 No Hit TGATTTTAAAATCCGCACCGTTGAGCAAGATGGAAAGACAATAAAGCTGC 8 0.2 No Hit GCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGACTTCCT 8 0.2 No Hit AGGATCGGGTTCGCGGAATCAGCTTGAGTGAAGAGACTGATGTTTGCAAA 8 0.2 No Hit GCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTTTGGCCGGGA 8 0.2 No Hit GACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTAC 8 0.2 No Hit TATTATCAGCTGATTGATCCACGTTAATTAGTTTGGCCGGGACGATATGA 8 0.2 No Hit CCTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGA 8 0.2 No Hit CAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTTTGGC 8 0.2 No Hit GGGCAACGCCCGCGGCGATGACGCCGTCCAGGAAGGGATCAACGACTGGA 8 0.2 No Hit GGACAGGATCGGGTTCGCGGAATCAGCTTGAGTGAAGAGACTGATGTTTG 8 0.2 No Hit GAACAACCGAAACCATTCATCCGTCCTTCTCAGTAACCGTCGTTTCAAGC 8 0.2 No Hit CTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAGCATGTT 8 0.2 No Hit CAAGTGTCGGTCCACCACCAAGGCTTGATCATGTAACCGTGACCCTGCCC 7 0.17500000000000002 No Hit CTATGATGTGACTGACCAGGAGAGCTTCAACAACGTCAAGCAGTGGCTGA 7 0.17500000000000002 No Hit CTCACCTGCCGAGCTGTTCTTGCTTGACCCAGCAGAAGAAAAACCGACAT 7 0.17500000000000002 No Hit GACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGACTT 7 0.17500000000000002 No Hit TGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGGTGGTC 7 0.17500000000000002 No Hit AATGGAGGCAGTTGGCGACAACTGGGTTTCCAAGTGTCGGTCCACCACCA 7 0.17500000000000002 No Hit GCAAAGAAGGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCT 7 0.17500000000000002 No Hit GCCGTGTTGTTGCACATTGACAGTAGAGACACCAAAAATTGTGTCCACCG 7 0.17500000000000002 No Hit GTTGAGCAAGATGGAAAGACAATAAAGCTGCAAATTTGGGACACTGCTGG 7 0.17500000000000002 No Hit ACCGAAACCATTCATCCGTCCTTCTCAGTAACCGTCGTTTCAAGCAAGCA 7 0.17500000000000002 No Hit GTTGGCGACAACTGGGTTTCCAAGTGTCGGTCCACCACCAAGGCTTGATC 7 0.17500000000000002 No Hit AGCTGATTGATCCACGTTAATTAGTTTGGCCGGGACGATATGAGTTCCTC 7 0.17500000000000002 No Hit TGTTGTTGCACATTGACAGTAGAGACACCAAAAATTGTGTCCACCGTGTG 7 0.17500000000000002 No Hit ATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGGTG 7 0.17500000000000002 No Hit GAGGCAAAGAAGGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGT 7 0.17500000000000002 No Hit AGAAAAACCGACATGGAGGATTCTGAATGTCCCTGGTCAGCCGCCGAAGT 7 0.17500000000000002 No Hit CGAAAGGGAGATTCAGGAAATTTTGGAGAAACTACAAGATTCTTCAGTTG 7 0.17500000000000002 No Hit GTGAAAATGTGAACAAGCTTTTGGTTGGGAACAAATGCGACCTGGCTGAG 7 0.17500000000000002 No Hit CCGCATCTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAG 7 0.17500000000000002 No Hit ACCATCACAAGCAGCTACTACCGTGGTGCCCATGGCATCATTGTAGTCTA 7 0.17500000000000002 No Hit GGATGATTCATACCTGGAGAGCTATATCAGTACTATTGGTGTTGATTTTA 7 0.17500000000000002 No Hit ACTCCCCCGCCGCCGCCGCCATGAATCCCGAATATGACTACCTCTTCAAG 6 0.15 No Hit AAGCAGTGGCTGAATGAAATTGACAGGTATGCCAGTGAAAATGTGAACAA 6 0.15 No Hit AAGAAGGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCC 6 0.15 No Hit TGAAGCTGGAACAATCAGGGCAAACTGTCTGCATCAGTGGGTTCATGGCA 6 0.15 No Hit GGCGAGGTGACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGA 6 0.15 No Hit ATGGCATCATTGTAGTCTATGATGTGACTGACCAGGAGAGCTTCAACAAC 6 0.15 No Hit GAAGGTGATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCG 6 0.15 No Hit GCGATTTAGGACCATCACAAGCAGCTACTACCGTGGTGCCCATGGCATCA 6 0.15 No Hit GGAAATTTTGGAGAAACTACAAGATTCTTCAGTTGCAGAAGGCTCTCGTA 6 0.15 No Hit CTGACGCCGACATCGTCGTCCTGCCCGTCGGGGCGAAGGTTACCGATGAC 6 0.15 No Hit TCTCACCTTCCTCCTCCTCGGTCGTCGCCCTTCGTCTCCCAGGAAATCGC 6 0.15 No Hit GCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTTTGGCCGG 6 0.15 No Hit GCTGAATGAAATTGACAGGTATGCCAGTGAAAATGTGAACAAGCTTTTGG 6 0.15 No Hit GATTGATCCACGTTAATTAGTTTGGCCGGGACGATATGAGTTCCTCCTCC 6 0.15 No Hit TATTATTATCAGCTGATTGATCCACGTTAATTAGTTTGGCCGGGACGATA 6 0.15 No Hit CAGCTGATTGATCCACGTTAATTAGTTTGGCCGGGACGATATGAGTTCCT 6 0.15 No Hit ATGAAATTGACAGGTATGCCAGTGAAAATGTGAACAAGCTTTTGGTTGGG 6 0.15 No Hit ACACCAAGCGCTAATATTATTATCAGCTGATTGATCCACGTTAATTAGTT 6 0.15 No Hit GCGTCCGCATCTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAG 6 0.15 No Hit CGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGCCGGAGG 6 0.15 No Hit CCGGGACGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGTGGC 6 0.15 No Hit GTGCCCATGGCATCATTGTAGTCTATGATGTGACTGACCAGGAGAGCTTC 6 0.15 No Hit CAGCTCGGGTGGCGCGGGCCCGTCCCGGACTCAGAAGGGCACCGCATCCG 6 0.15 No Hit GCAGAATCTCCTTGTCATGTACTGGCATGCATTAGGATATTGTCCGTGAT 6 0.15 No Hit GTTTGGCCGGGACGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGT 6 0.15 No Hit TTTAAAATCCGCACCGTTGAGCAAGATGGAAAGACAATAAAGCTGCAAAT 5 0.125 No Hit CATCCTTGGTCCTAGAGGAAATTCATTGAAGCGAGTGGAGGCAACTACTG 5 0.125 No Hit TGATTCACGTTAATTAGTTTGGCCGGGACGATATGAGTTCCTCCTCCGTC 5 0.125 No Hit GCCTAGAACAACCGAAACCATTCATCCGTCCTTCTCAGTAACCGTCGTTT 5 0.125 No Hit GCGATCAGATGGCGCAGGAGAGCCTGAGGCTGGTGTCGCCGGCGATCTCG 5 0.125 No Hit TCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAGCATGTTCC 5 0.125 No Hit CTTCCTGCCCAACCGCGTCCGCATCTTCGTCGACACCGTCGCCGAGATCC 5 0.125 No Hit CATCTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAGCAT 5 0.125 No Hit CGTCCTGCCCGTCGGGGCGAAGGTTACCGATGACTTCCTGCCCAACCGCG 5 0.125 No Hit GTCCATCAAGGAGGCAAAGAAGGTGATCCTCAAGGACAAGCCTGACGCCG 5 0.125 No Hit GGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCAAAGAAGGTGATCCTC 5 0.125 No Hit GAGCGCTGCTTCCAGAGGTTAGAAGCGCCGGTCGCAAGGGTCTGCGGCCT 5 0.125 No Hit CCGACACCAAGGGTTTGCCCGAGGGCTTCTCCGGCGCCGGGGGCAACGCC 5 0.125 No Hit CTCACCTTCCTCCTCCTCGGTCGTCGCCCTTCGTCTCCCAGGAAATCGCC 5 0.125 No Hit CGACTTCGGCGAGGACAGGATCGGGTTCGCGGAATCAGCTTGAGTGAAGA 5 0.125 No Hit CGGCGAGGACAGGATCGGGTTCGCGGAATCAGCTTGAGTGAAGAGACTGA 5 0.125 No Hit GAGCTGTTCTTGCTTGACCCAGCAGAAGAAAAACCGACATGGAGGATTCT 5 0.125 No Hit GAAAAACCGACATGGAGGATTCTGAATGTCCCTGGTCAGCCGCCGAAGTT 5 0.125 No Hit GGACCATCACAAGCAGCTACTACCGTGGTGCCCATGGCATCATTGTAGTC 5 0.125 No Hit GGCAAAGAGTGGCTCGCTCCGGCTGCGCTCCAGTGATGCCTACACAATGG 5 0.125 No Hit CATCATATTTGGTGGCTCAATTGCTGGGCTGCACTCACCTGCCGAGCTGT 5 0.125 No Hit AGGTGACGAAGTCGTGGCCGGAGGTGGTCGGGCTGTCCATCAAGGAGGCA 5 0.125 No Hit TCTGAATGTCCCTGGTCAGCCGCCGAAGTTCGCTTGGGGGCATAGTACCT 5 0.125 No Hit GCAGAAGAAAAACCGACATGGAGGATTCTGAATGTCCCTGGTCAGCCGCC 5 0.125 No Hit GTTTGCAAAGATTTCCCTCGTATCGTAGAATAGTTTGGCTATCTCTGATA 5 0.125 No Hit GCTATATCAGTACTATTGGTGTTGATTTTAAAATCCGCACCGTTGAGCAA 5 0.125 No Hit GGAGAATCAACAGTCAGCTGCCATGAGATCTCCAAGATGCCAAATCTTGC 5 0.125 No Hit TGGCCGGGACGATATGAGTTCCTCCTCCGTCGGCGAGGTGACGAAGTCGT 5 0.125 No Hit CTGACCAGGAGAGCTTCAACAACGTCAAGCAGTGGCTGAATGAAATTGAC 5 0.125 No Hit GACTTCGGCGAGGACAGGATCGGGTTCGCGGAATCAGCTTGAGTGAAGAG 5 0.125 No Hit CTTCGTGCGATGCGTATGTTTCTGAATTGCTTAGCAATACCAAAGAGGAA 5 0.125 No Hit CAGGAAATCGCCTGCCGCCGCCCGCCGACCAGGAGGACTCCCCCGCCGCC 5 0.125 No Hit GGTTTGCGGATGATTCATACCTGGAGAGCTATATCAGTACTATTGGTGTT 5 0.125 No Hit ATCATTGTAGTCTATGATGTGACTGACCAGGAGAGCTTCAACAACGTCAA 5 0.125 No Hit GATCCTCAAGGACAAGCCTGACGCCGACATCGTCGTCCTGCCCGTCGGGG 5 0.125 No Hit GAAAATGTGAACAAGCTTTTGGTTGGGAACAAATGCGACCTGGCTGAGAG 5 0.125 No Hit CGCATCTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGGCTAGCTAGC 5 0.125 No Hit AGAGAACCAGCTCCGTGAAAACAAAACAAAGGAGCTGATACTGCAATATG 5 0.125 No Hit GCTAGAAAATCCGCACCGTTGAGCAAGATGGAAAGACAATAAAGCTGCAA 5 0.125 No Hit GGATCATCATATTTGGTGGCTCAATTGCTGGGCTGCACTCACCTGCCGAG 5 0.125 No Hit ATGTGACTGACCAGGAGAGCTTCAACAACGTCAAGCAGTGGCTGAATGAA 5 0.125 No Hit AACCGCGTCCGCATCTTCGTCGACACCGTCGCCGAGATCCCCCGTGCTGG 5 0.125 No Hit GTTTGCGGATGATTCATACCTGGAGAGCTATATCAGTACTATTGGTGTTG 5 0.125 No Hit ACACAGTTAGCCGCCGGCTAGCTAGCCGTATGTCCGTACGCACGCGTACA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGCTA 65 0.008563579 21.925001 9 CGCTAAT 70 8.3723967E-4 14.25125 10-14 GCTAATA 70 8.3723967E-4 14.25125 10-14 GCGCTAA 70 8.3723967E-4 14.25125 10-14 TATTATT 70 8.3723967E-4 14.25125 15-19 AATATTA 70 8.3723967E-4 14.25125 15-19 ATATTAT 70 8.3723967E-4 14.25125 15-19 CGTTAAT 75 0.0014104045 13.301167 40-44 ACGTTAA 75 0.0014104045 13.301167 40-44 TGATTGA 75 0.0014104045 13.301167 30-34 TTATCAG 75 0.0014104045 13.301167 20-24 GATTGAT 75 0.0014104045 13.301167 30-34 TCAGCTG 75 0.0014104045 13.301167 25-29 ATTATCA 75 0.0014104045 13.301167 20-24 GATCCAC 75 0.0014104045 13.301167 35-39 ATTGATC 75 0.0014104045 13.301167 30-34 TATTATC 75 0.0014104045 13.301167 20-24 AGCTGAT 75 0.0014104045 13.301167 25-29 CAGCTGA 75 0.0014104045 13.301167 25-29 TCCACGT 80 0.0022927988 12.469844 35-39 >>END_MODULE Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485745 spots for ERR5262796.sra Written 1485745 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra Read 1485736 spots for ERR5262796.sra Written 1485736 spots for ERR5262796.sra SRR ids: ['ERR5262796.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_vzrs1k9v ERR5262796.sra spots: 29714729 blocks: [[1, 1485736], [1485737, 2971472], [2971473, 4457208], [4457209, 5942944], [5942945, 7428680], [7428681, 8914416], [8914417, 10400152], [10400153, 11885888], [11885889, 13371624], [13371625, 14857360], [14857361, 16343096], [16343097, 17828832], [17828833, 19314568], [19314569, 20800304], [20800305, 22286040], [22286041, 23771776], [23771777, 25257512], [25257513, 26743248], [26743249, 28228984], [28228985, 29714729]] ERR5262796 file size 9734688 ERR5262796 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262796 ERR5262796_1.fastq ERR5262796_2.fastq Input file: ERR5262796_1.fastq Paired file: ERR5262796_2.fastq trimmed: ERR5262796-trimmed-pair1.fastq, ERR5262796-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:57:06 2024 >> started Fri Dec 6 11:57:39 2024 >> done (32.641s) 29714729 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 29714728 (100.00%) read pairs available; of these: 9483 ( 0.03%) trimmed read pairs available after processing 29705245 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 22 2 0.00% 23 0 0.00% 24 0 0.00% 25 1 0.00% 26 1 0.00% 27 2 0.00% 28 1 0.00% 29 0 0.00% 30 1 0.00% 31 2 0.00% 32 0 0.00% 33 5 0.00% 34 1 0.00% 35 3 0.00% 36 1 0.00% 37 1 0.00% 38 3 0.00% 39 2 0.00% 40 0 0.00% 41 2 0.00% 42 1 0.00% 43 3 0.00% 44 5 0.00% 45 3 0.00% 46 3 0.00% 47 1 0.00% 48 0 0.00% 49 346 0.00% 50 349 0.00% 51 387 0.00% 52 422 0.00% 53 435 0.00% 54 470 0.00% 55 549 0.00% 56 594 0.00% 57 703 0.00% 58 844 0.00% 59 897 0.00% 60 1101 0.00% 61 1247 0.00% 62 1431 0.00% 63 1575 0.01% 64 1645 0.01% 65 1947 0.01% 66 2075 0.01% 67 2298 0.01% 68 2657 0.01% 69 3023 0.01% 70 3615 0.01% 71 4000 0.01% 72 4838 0.02% 73 5271 0.02% 74 5934 0.02% 75 6540 0.02% 76 6936 0.02% 77 7500 0.03% 78 8439 0.03% 79 9453 0.03% 80 10441 0.04% 81 11932 0.04% 82 13692 0.05% 83 15084 0.05% 84 15892 0.05% 85 17866 0.06% 86 18404 0.06% 87 19486 0.07% 88 20938 0.07% 89 22328 0.08% 90 24345 0.08% 91 26210 0.09% 92 28752 0.10% 93 31009 0.10% 94 32851 0.11% 95 35145 0.12% 96 36754 0.12% 97 37717 0.13% 98 38853 0.13% 99 40200 0.14% 100 42788 0.14% 101 44466 0.15% 102 46696 0.16% 103 50119 0.17% 104 52550 0.18% 105 54692 0.18% 106 56503 0.19% 107 57227 0.19% 108 58131 0.20% 109 59701 0.20% 110 61540 0.21% 111 63903 0.22% 112 67000 0.23% 113 69365 0.23% 114 73040 0.25% 115 75128 0.25% 116 76111 0.26% 117 77094 0.26% 118 79295 0.27% 119 79548 0.27% 120 79784 0.27% 121 82040 0.28% 122 83948 0.28% 123 86564 0.29% 124 91086 0.31% 125 92178 0.31% 126 93943 0.32% 127 94781 0.32% 128 95561 0.32% 129 96679 0.33% 130 96094 0.32% 131 97501 0.33% 132 99638 0.34% 133 102372 0.34% 134 105202 0.35% 135 106728 0.36% 136 109480 0.37% 137 108625 0.37% 138 109787 0.37% 139 112484 0.38% 140 113621 0.38% 141 116672 0.39% 142 119653 0.40% 143 122654 0.41% 144 123316 0.41% 145 123763 0.42% 146 129649 0.44% 147 262566 0.88% 148 116498 0.39% 149 117275 0.39% 150 24594225 82.77% 29714728 reads passed initial QC criterion=sequence-density sequence-density=2.16 sequence-density-rank=1 fanout-score=2.43 fanout-score-rank=27 prefix-density=2.38 prefix-fanout=2.2 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=193.36 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=2.4 sequence=CTTCACAGCAATGTGGGAGGTGTGGCAGTCCAGCACTGGGGCGTAGCCGTTGCCAATCTGACCAGGGTGGTTCATGATGATGACCTGGGAGGTGAAGTTGGCAGCCTCCTTGGCAGGGTCATCCTTGGAGTTGGATGCAACAAACCCACGCTTGAGATCCTTCACAGCAACGTTCTTGACGTTGAAGCCAACATTGTCACCAGGAAGGGCCTCCTGGAGAGCCTCATGGTGCATCTCAACGGACTTGACCTCAGTGGTAAGACCAGTAGGACCGAAGGTAACAATCATACCAGGCTTGATGACACCAGTCTCAACACGGCCAACAGGCACAGTTCCAATGCCACCAATCTTGTACACGTCCTGGAGGGGAAGACGCAGGGGCTTGTCCGAGGGCCTCTTGGGCTCATTGATCTGGTCAAGTGCCTCAAGCAAGGTAGGGCCCTTGTACCAGTCAAGGTTGGTGGACCTCTCAATCATGTTGTCACCCTCAAAACCAGAGATGGG criterion=sequence-density sequence-density=7.96 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=39 prefix-density=7.92 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.39 sequence-density-rank=15 fanout-score=46.51 fanout-score-rank=1 prefix-density=8.29 prefix-fanout=2.2 sequence=GTTCCGGTTCGCGGCTAGCAGTAGTTGTTGTAGTAGCAGCTAGGGTTTCCGGTAGGGTTCCGTCGAGATCGCCATGGATGAGTACCGCTGCTTCGTGGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262796 ERR5262796_1.fastq ERR5262796_2.fastq Input file: ERR5262796_1.fastq Paired file: ERR5262796_2.fastq trimmed: ERR5262796-trimmed-pair1.fastq, ERR5262796-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:59:40 2024 >> started Fri Dec 6 12:00:00 2024 >> done (20.167s) 19809819 read pairs processed; of these: 82 ( 0.00%) short read pairs filtered out after trimming by size control 399 ( 0.00%) empty read pairs filtered out after trimming by size control 19809338 (100.00%) read pairs available; of these: 151 ( 0.00%) trimmed read pairs available after processing 19809187 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 25 1 0.00% 26 1 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 1 0.00% 31 2 0.00% 32 0 0.00% 33 4 0.00% 34 1 0.00% 35 3 0.00% 36 0 0.00% 37 1 0.00% 38 3 0.00% 39 2 0.00% 40 0 0.00% 41 1 0.00% 42 1 0.00% 43 3 0.00% 44 2 0.00% 45 1 0.00% 46 3 0.00% 47 1 0.00% 48 0 0.00% 49 226 0.00% 50 257 0.00% 51 212 0.00% 52 196 0.00% 53 225 0.00% 54 257 0.00% 55 276 0.00% 56 331 0.00% 57 401 0.00% 58 445 0.00% 59 481 0.00% 60 584 0.00% 61 714 0.00% 62 830 0.00% 63 1037 0.01% 64 1102 0.01% 65 1389 0.01% 66 1547 0.01% 67 1629 0.01% 68 1876 0.01% 69 2188 0.01% 70 2631 0.01% 71 2641 0.01% 72 3236 0.02% 73 3677 0.02% 74 4112 0.02% 75 3604 0.02% 76 4099 0.02% 77 5436 0.03% 78 6234 0.03% 79 6319 0.03% 80 6697 0.03% 81 8761 0.04% 82 9578 0.05% 83 10310 0.05% 84 9259 0.05% 85 12917 0.07% 86 11782 0.06% 87 12690 0.06% 88 15332 0.08% 89 13602 0.07% 90 16293 0.08% 91 16461 0.08% 92 19085 0.10% 93 20783 0.10% 94 23822 0.12% 95 24199 0.12% 96 25609 0.13% 97 24567 0.12% 98 26285 0.13% 99 27082 0.14% 100 26463 0.13% 101 30428 0.15% 102 31190 0.16% 103 32971 0.17% 104 35799 0.18% 105 36127 0.18% 106 36570 0.18% 107 37560 0.19% 108 38359 0.19% 109 38437 0.19% 110 41409 0.21% 111 41314 0.21% 112 44903 0.23% 113 46437 0.23% 114 47673 0.24% 115 49825 0.25% 116 49882 0.25% 117 50708 0.26% 118 52753 0.27% 119 53665 0.27% 120 52922 0.27% 121 55724 0.28% 122 55557 0.28% 123 57960 0.29% 124 60766 0.31% 125 61427 0.31% 126 62449 0.32% 127 63141 0.32% 128 63786 0.32% 129 64272 0.32% 130 64086 0.32% 131 64907 0.33% 132 66441 0.34% 133 68026 0.34% 134 69987 0.35% 135 71139 0.36% 136 73550 0.37% 137 72557 0.37% 138 73602 0.37% 139 75014 0.38% 140 75850 0.38% 141 77913 0.39% 142 79996 0.40% 143 81869 0.41% 144 82160 0.41% 145 83130 0.42% 146 86665 0.44% 147 175177 0.88% 148 77585 0.39% 149 78740 0.40% 150 16397130 82.77% criterion=sequence-density sequence-density=0.16 sequence-density-rank=1 fanout-score=3.45 fanout-score-rank=31 prefix-density=0.21 prefix-fanout=2.5 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.04 sequence-density-rank=30 fanout-score=313.95 fanout-score-rank=1 prefix-density=0.58 prefix-fanout=19.1 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTG criterion=sequence-density sequence-density=0.70 sequence-density-rank=1 fanout-score=2.14 fanout-score-rank=36 prefix-density=0.71 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=24 fanout-score=256.56 fanout-score-rank=1 prefix-density=0.96 prefix-fanout=21.1 sequence=CGCCGCCGCCGTCG ERR5262796 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:01:02 Started mapping on | Dec 06 12:01:02 Finished on | Dec 06 12:03:41 Mapping speed, Million of reads per hour | 672.78 Number of input reads | 29714247 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 28481651 Uniquely mapped reads % | 95.85% Average mapped length | 290.40 Number of splices: Total | 26063231 Number of splices: Annotated (sjdb) | 24084803 Number of splices: GT/AG | 25673635 Number of splices: GC/AG | 335179 Number of splices: AT/AC | 17312 Number of splices: Non-canonical | 37105 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.00% Deletion average length | 1.58 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 265695 % of reads mapped to multiple loci | 0.89% Number of reads mapped to too many loci | 1072 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.23% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 966901 966901 966901 N_multimapping 265695 265695 265695 N_noFeature 1383557 27622961 1695094 N_ambiguous 633323 4115 86185 UnstrandedReadsAssigned:26464771 PositiveStrandReadsAssigned:854575 NegativeStrandReadsAssigned:26700372 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262796 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262796-trimmed-pair1.fastq ERR5262796-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,714,247 reads, 27,145,567 reads pseudoaligned [quant] estimated average fragment length: 253.176 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,199 rounds 52973 ERR5262796.ke.tsv 35125 ERR5262796.se.tsv 88098 total ==> ERR5262796.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 684.437 0 0 PNS24247 1044 791.824 185.881 12.6673 PNS24249 1928 1675.82 432.887 13.9388 PNS24246 1044 791.824 185.881 12.6673 PNS24248 1044 791.824 185.881 12.6673 PNS24244 1471 1218.82 214.47 9.49519 PNS24243 293 105.157 0 0 KQK14069 1603 1350.82 61064.9 2439.33 KQK14071 474 244.817 366.818 80.8512 ==> ERR5262796.se.tsv <== BRADI_1g14170v3 61599 BRADI_1g53295v3 269 BRADI_1g59795v3 1014 BRADI_1g07683v3 0 BRADI_1g00485v3 28 BRADI_1g20270v3 615 BRADI_1g74790v3 2413 BRADI_1g09890v3 0 BRADI_1g77505v3 396 BRADI_1g48960v3 0 ERR5262796 completed mapping pipeline successfully