Starting /dee2/code/volunteer_pipeline.sh ERR5262797 current disk space = 1551425114112 free memory = 1602186308 ERR5262797 SRAfilesize df0c0b9fbe76812268456e55c5134645 ERR5262797.sra ERR5262797.sra file validated ERR5262797 is paired end ERR5262797 is conventional basespace ERR5262797 read1 length is 51-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262797_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 51-150 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.681 37.0 37.0 37.0 37.0 37.0 2 36.45175 37.0 37.0 37.0 37.0 37.0 3 36.6725 37.0 37.0 37.0 37.0 37.0 4 36.749 37.0 37.0 37.0 37.0 37.0 5 36.7065 37.0 37.0 37.0 37.0 37.0 6 36.6455 37.0 37.0 37.0 37.0 37.0 7 36.625 37.0 37.0 37.0 37.0 37.0 8 36.685 37.0 37.0 37.0 37.0 37.0 9 36.73 37.0 37.0 37.0 37.0 37.0 10-14 36.6952 37.0 37.0 37.0 37.0 37.0 15-19 36.5569 37.0 37.0 37.0 37.0 37.0 20-24 36.611599999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.606700000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.5981 37.0 37.0 37.0 37.0 37.0 35-39 36.618900000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.547399999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.6379 37.0 37.0 37.0 37.0 37.0 50-54 36.5935432608152 37.0 37.0 37.0 37.0 37.0 55-59 36.65041260315079 37.0 37.0 37.0 37.0 37.0 60-64 36.634608652163045 37.0 37.0 37.0 37.0 37.0 65-69 36.61180295073768 37.0 37.0 37.0 37.0 37.0 70-74 36.55478869717429 37.0 37.0 37.0 37.0 37.0 75-79 36.57748874437218 37.0 37.0 37.0 37.0 37.0 80-84 36.53862134970412 37.0 37.0 37.0 37.0 37.0 85-89 36.49433465611578 37.0 37.0 37.0 37.0 37.0 90-94 36.45637656668652 37.0 37.0 37.0 37.0 37.0 95-99 36.42812263930496 37.0 37.0 37.0 37.0 37.0 100-104 36.45717262361929 37.0 37.0 37.0 37.0 37.0 105-109 36.474856478596635 37.0 37.0 37.0 37.0 37.0 110-114 36.41585789660376 37.0 37.0 37.0 37.0 37.0 115-119 36.40824369530397 37.0 37.0 37.0 37.0 37.0 120-124 36.28419163960765 37.0 37.0 37.0 37.0 37.0 125-129 36.38253699154224 37.0 37.0 37.0 37.0 37.0 130-134 36.45366689878048 37.0 37.0 37.0 37.0 37.0 135-139 36.273210746880174 37.0 37.0 37.0 37.0 37.0 140-144 36.22087977071534 37.0 37.0 37.0 37.0 37.0 145-149 36.002758429097376 37.0 37.0 37.0 37.0 37.0 150 35.96639904846863 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 5.0 28 7.0 29 9.0 30 9.0 31 19.0 32 25.0 33 54.0 34 89.0 35 200.0 36 2994.0 37 588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85 16.25 10.85 29.049999999999997 2 26.081520380095025 9.32733183295824 39.75993998499625 24.831207801950487 3 28.499999999999996 15.525 33.050000000000004 22.925 4 26.924999999999997 22.775000000000002 25.45 24.85 5 20.424999999999997 32.375 29.7 17.5 6 24.725 32.1 24.4 18.775 7 19.1 25.5 46.400000000000006 9.0 8 15.8 30.9 34.375 18.925 9 22.400000000000002 30.725 28.225 18.65 10-14 24.7 26.365 31.430000000000003 17.505000000000003 15-19 25.555 31.474999999999998 26.365 16.605 20-24 15.89 34.1 27.334999999999997 22.675 25-29 26.525 30.31 26.045 17.119999999999997 30-34 23.13 24.9 27.66 24.310000000000002 35-39 16.64 27.134999999999998 27.529999999999998 28.694999999999997 40-44 21.060000000000002 22.46 33.165 23.315 45-49 20.53 26.845000000000002 26.650000000000002 25.974999999999998 50-54 26.80402060309046 23.258488773315996 23.128469270390557 26.809021353202983 55-59 19.80495123780945 21.330332583145786 37.164291072768194 21.70042510627657 60-64 26.171542885721433 25.76144036009002 24.42110527631908 23.645911477869465 65-69 26.041510377594403 30.807701925481368 20.960240060015003 22.190547636909226 70-74 21.21530382595649 30.752688172043012 22.90072518129532 25.131282820705174 75-79 20.645322661330663 28.88944472236118 26.483241620810404 23.98199099549775 80-84 28.216930158094854 27.181308785271163 19.736842105263158 24.864918951370825 85-89 23.60310699072914 24.074166875469807 23.688298672012024 28.634427461789024 90-94 25.420579520915986 25.57123487169186 29.18696329031286 19.821222317079297 95-99 27.870505773788512 26.599768039937473 20.47803943321063 25.051686753063386 100-104 24.667681380010148 24.885844748858446 28.340943683409435 22.10553018772197 105-109 21.092115335544783 31.227353916815513 25.792293952538913 21.88823679510079 110-114 20.61478157718294 31.033059982464284 22.693279694672235 25.658878745680543 115-119 25.193879689792496 26.713477258436384 19.712848459442466 28.37979459232865 120-124 17.981709910676308 29.52467035304126 23.45810293492131 29.035516801361123 125-129 24.920369270636506 27.56033040004319 20.92533606867138 26.59396426064892 130-134 23.101022315048915 20.567219962625042 24.101352094096956 32.230405628229086 135-139 21.155690988093223 22.75830935048812 30.63596862479544 25.450031036623216 140-144 21.812446228850014 27.232578147404645 22.632635503297962 28.322340120447375 145-149 23.756582796957286 23.680514921006438 31.76711527208894 20.795787009947336 150 26.04817127564674 20.48765982753494 33.51174546535831 19.952423431460005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 2.0 32 2.5 33 2.5 34 5.5 35 15.0 36 11.5 37 1.0 38 8.0 39 97.5 40 115.5 41 99.5 42 97.5 43 173.5 44 208.5 45 380.0 46 514.5 47 422.5 48 526.0 49 391.0 50 171.0 51 157.5 52 191.5 53 252.5 54 148.5 55 6.5 56 0.5 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 50-51 1.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0 84-85 5.0 86-87 2.0 88-89 5.0 90-91 0.0 92-93 6.0 94-95 10.0 96-97 6.0 98-99 8.0 100-101 15.0 102-103 7.0 104-105 9.0 106-107 8.0 108-109 15.0 110-111 23.0 112-113 24.0 114-115 29.0 116-117 18.0 118-119 27.0 120-121 20.0 122-123 22.0 124-125 24.0 126-127 22.0 128-129 17.0 130-131 27.0 132-133 57.0 134-135 33.0 136-137 26.0 138-139 22.0 140-141 20.0 142-143 34.0 144-145 29.0 146-147 18.0 148-149 46.0 150-151 3363.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.725 #Duplication Level Percentage of deduplicated Percentage of total 1 34.95702005730659 3.05 2 15.18624641833811 2.65 3 8.595988538681947 2.25 4 6.59025787965616 2.3 5 3.7249283667621778 1.625 6 3.151862464183381 1.6500000000000001 7 3.151862464183381 1.925 8 1.146131805157593 0.8 9 0.5730659025787965 0.44999999999999996 >10 16.9054441260745 32.05 >50 4.011461318051576 24.375 >100 2.005730659025788 26.875 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTCAATAAATTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACTG 237 5.925 No Hit GTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACC 199 4.9750000000000005 No Hit CCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCG 163 4.075 No Hit GTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTA 124 3.1 No Hit GCGATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGGGGA 119 2.9749999999999996 No Hit GTTGTGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAA 119 2.9749999999999996 No Hit ATTTTGTTTATGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTG 114 2.85 No Hit CTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCAC 100 2.5 No Hit GGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTGG 96 2.4 No Hit GTGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCT 82 2.0500000000000003 No Hit TGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCC 82 2.0500000000000003 No Hit GCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCA 69 1.725 No Hit GTTGCGATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGG 68 1.7000000000000002 No Hit GGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTGGC 67 1.675 No Hit AGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAACTC 67 1.675 No Hit GCTTGAGCTAATATGCTGCCATCAGACTACGCTGAAGCTGGTATTCTTAT 66 1.6500000000000001 No Hit ACCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCC 62 1.55 No Hit GAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAACTCC 56 1.4000000000000001 No Hit AGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAACTCCC 54 1.35 No Hit CGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTG 53 1.325 No Hit CGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTTCAAAGT 53 1.325 No Hit GCCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCC 49 1.225 No Hit GGAGGCTTGAGCTAATATGCTGCCATCAGACTACGCTGAAGCTGGTATTC 45 1.125 No Hit CTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGC 44 1.0999999999999999 No Hit ACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCT 44 1.0999999999999999 No Hit CTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTTCA 44 1.0999999999999999 No Hit GTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCAT 39 0.975 No Hit CCTTCAATAAATTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACT 39 0.975 No Hit GCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTTCAAAG 37 0.9249999999999999 No Hit GGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTTCAAA 36 0.8999999999999999 No Hit GACAAACAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGC 34 0.8500000000000001 No Hit GGCTTGAGCTAATATGCTGCCATCAGACTACGCTGAAGCTGGTATTCTTA 32 0.8 No Hit ACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTGGAA 30 0.75 No Hit TTTTTTTTTTTTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACTG 29 0.7250000000000001 No Hit ACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCG 28 0.7000000000000001 No Hit GTTTATGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTGAACGT 28 0.7000000000000001 No Hit GCTGATTCAACTAGTTTCTCCCTGGCAAAATCCATAAATGTTCTATCACG 28 0.7000000000000001 No Hit GGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTGGCG 26 0.65 No Hit CCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGG 25 0.625 No Hit CCCAAATTTATTTATCAGAGGAAGGAGGCTTGAGCTAATATGCTGCCATC 24 0.6 No Hit AGACAAACAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGG 23 0.575 No Hit CACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTGGA 22 0.5499999999999999 No Hit CAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAACT 22 0.5499999999999999 No Hit CCCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCC 21 0.525 No Hit TTTTTTTTTTTTTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACT 21 0.525 No Hit TGTGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGC 21 0.525 No Hit GAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTGGCGT 21 0.525 No Hit TTTTTTTTTTTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACTGA 21 0.525 No Hit CGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTT 20 0.5 No Hit CTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGC 20 0.5 No Hit GTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAA 20 0.5 No Hit GATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGGGGAAT 19 0.475 No Hit GCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCAT 18 0.44999999999999996 No Hit CGTTGTGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAA 17 0.42500000000000004 No Hit TGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAACTC 17 0.42500000000000004 No Hit GGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAACTC 17 0.42500000000000004 No Hit TGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTA 16 0.4 No Hit CTCATAATATGTTTTCTATCGCTGATTCAACTAGTTTCTCCCTGGCAAAA 15 0.375 No Hit TGCGATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGGGG 15 0.375 No Hit GGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCC 15 0.375 No Hit ACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTT 15 0.375 No Hit GGAAGGAGGCTTGAGCTAATATGCTGCCATCAGACTACGCTGAAGCTGGT 15 0.375 No Hit GGAACCACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAA 14 0.35000000000000003 No Hit CGCTGATTCAACTAGTTTCTCCCTGGCAAAATCCATAAATGTTCTATCAC 14 0.35000000000000003 No Hit CTCCCTGGCAAAATCCATAAATGTTCTATCACGGTATTGAAAGATCAGTC 13 0.325 No Hit AGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCC 13 0.325 No Hit TCAATAAATTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACTGAT 12 0.3 No Hit AGAGGAAGGAGGCTTGAGCTAATATGCTGCCATCAGACTACGCTGAAGCT 12 0.3 No Hit AACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGT 12 0.3 No Hit TAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACT 12 0.3 No Hit AACAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAA 12 0.3 No Hit GTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTC 12 0.3 No Hit CGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCC 11 0.27499999999999997 No Hit CACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGC 11 0.27499999999999997 No Hit CAAACAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCA 11 0.27499999999999997 No Hit CGATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGGGGAA 11 0.27499999999999997 No Hit CTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATT 10 0.25 No Hit AGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTT 10 0.25 No Hit TCCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCC 10 0.25 No Hit AAACAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAA 10 0.25 No Hit TTTGTTTATGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTGAA 9 0.22499999999999998 No Hit GTGCTGGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACC 9 0.22499999999999998 No Hit ATCAACGTTGTGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACC 8 0.2 No Hit ACCACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTG 8 0.2 No Hit TGTTTATGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTGAACG 8 0.2 No Hit CCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTTC 8 0.2 No Hit TTTTTAAATTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACTGAT 7 0.17500000000000002 No Hit GAGGAAGGAGGCTTGAGCTAATATGCTGCCATCAGACTACGCTGAAGCTG 7 0.17500000000000002 No Hit AGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCA 7 0.17500000000000002 No Hit TTCAATAAATTAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACTGA 7 0.17500000000000002 No Hit CCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTGGAAC 7 0.17500000000000002 No Hit TTGTGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAG 7 0.17500000000000002 No Hit CAGACAAACAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTG 7 0.17500000000000002 No Hit GGAACTCATAATATGTTTTCTATCGCTGATTCAACTAGTTTCTCCCTGGC 7 0.17500000000000002 No Hit TTTTGTTTATGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTGA 7 0.17500000000000002 No Hit TCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTTC 7 0.17500000000000002 No Hit ATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGGGGAATA 7 0.17500000000000002 No Hit GTCAGAGGAAGGAGGCTTGAGCTAATATGCTGCCATCAGACTACGCTGAA 6 0.15 No Hit TTATGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTGAACGTGT 6 0.15 No Hit AGGAAATACAACTAAATGCAGGATGTTCCACAGAAACATAGACCGAATTC 6 0.15 No Hit CACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGT 6 0.15 No Hit GACAGACAAACAGAGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCAC 6 0.15 No Hit GAACCACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAAC 6 0.15 No Hit GCGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCT 6 0.15 No Hit AAATTTATTTATCAGAGGAAGGAGGCTTGAGCTAATATGCTGCCATCAGA 6 0.15 No Hit ATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTT 6 0.15 No Hit CTTGAGCTAATATGCTGCCATCAGACTACGCTGAAGCTGGTATTCTTATC 6 0.15 No Hit GTGAAAATAGCTAACTGGAACTGCATTTGAGGCCGGCGCTGTTCAAAGTA 6 0.15 No Hit CGCGATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGGGG 5 0.125 No Hit TTTTTTTTTTTTGAGACAGACAAACAGAGCCTCTGCCTTTCTTATATACT 5 0.125 No Hit TACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGT 5 0.125 No Hit CCACCAAAGCTAGTTTACCCACTTCATCTGGCGTGAAAATAGCTAACTGG 5 0.125 No Hit GTGTGAACGTGTGCTAGTGTACGGGGAATAGTTTCTTGGAACCACCAAAG 5 0.125 No Hit TACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACGGGGAATAGT 5 0.125 No Hit AGGGCCTCTGCCTTTCTTATATACTGATGGTTGGTTCACTGGCCAAACTC 5 0.125 No Hit TGTACGGGGAATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCA 5 0.125 No Hit TTTATGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTGAACGTG 5 0.125 No Hit CCCTGGCAAAATCCATAAATGTTCTATCACGGTATTGAAAGATCAGTCTT 5 0.125 No Hit GGTTGCGATTACAACAACATATCAACGTTGTGAACGTGTGCTAGTGTACG 5 0.125 No Hit TGAACAAGTGTTGCGATTACAACAACATATCAACGTTGTGAACGTGTGCT 5 0.125 No Hit CCCGCTTCATCTGGCGTGAAAATAGCTAACTGGAACTGCATTTGAGGCCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTTA 10 0.007937102 137.90001 4 CTTCAAT 25 4.1463863E-8 137.90001 1 TGTTTAT 10 0.007937102 137.90001 5 ATTTTGT 10 0.007937102 137.90001 1 TTTGTTT 10 0.007937102 137.90001 3 TTTTGTT 10 0.007937102 137.90001 2 TAAATTA 30 1.2310193E-7 114.91667 7 TTCAATA 30 1.2310193E-7 114.91667 2 AAATTAG 30 1.2310193E-7 114.91667 8 AATTAGA 30 1.2310193E-7 114.91667 9 TCAATAA 30 1.2310193E-7 114.91667 3 CAATAAA 30 1.2310193E-7 114.91667 4 ATAAATT 30 1.2310193E-7 114.91667 6 AATAAAT 30 1.2310193E-7 114.91667 5 AAGTAAA 20 0.0029445752 33.4303 140-144 GCAAGTA 20 0.0029445752 33.4303 140-144 CAAGTAA 20 0.0029445752 33.4303 140-144 GTGCTAG 120 4.5468344E-4 28.729168 1 TAGACAG 35 5.229067E-6 27.580002 10-14 AGACAGA 40 4.645517E-7 27.58 10-14 >>END_MODULE ERR5262797 read2 length is 51-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262797_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 51-150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.145 37.0 37.0 37.0 37.0 37.0 2 35.997 37.0 37.0 37.0 37.0 37.0 3 36.1615 37.0 37.0 37.0 37.0 37.0 4 36.1395 37.0 37.0 37.0 37.0 37.0 5 36.242 37.0 37.0 37.0 37.0 37.0 6 36.279 37.0 37.0 37.0 37.0 37.0 7 36.179 37.0 37.0 37.0 37.0 37.0 8 36.3395 37.0 37.0 37.0 37.0 37.0 9 36.3165 37.0 37.0 37.0 37.0 37.0 10-14 36.2833 37.0 37.0 37.0 37.0 37.0 15-19 36.286 37.0 37.0 37.0 37.0 37.0 20-24 36.2479 37.0 37.0 37.0 37.0 37.0 25-29 36.2697 37.0 37.0 37.0 37.0 37.0 30-34 36.1982 37.0 37.0 37.0 37.0 37.0 35-39 36.2128 37.0 37.0 37.0 37.0 37.0 40-44 36.22239999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.174299999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.11166409102275 37.0 37.0 37.0 37.0 37.0 55-59 36.11837959489873 37.0 37.0 37.0 37.0 37.0 60-64 36.13108277069267 37.0 37.0 37.0 37.0 37.0 65-69 36.18989747436859 37.0 37.0 37.0 37.0 37.0 70-74 36.14208552138034 37.0 37.0 37.0 37.0 37.0 75-79 36.10005002501251 37.0 37.0 37.0 37.0 37.0 80-84 36.091149552759866 37.0 37.0 37.0 37.0 37.0 85-89 36.049383300639654 37.0 37.0 37.0 37.0 37.0 90-94 36.01159983262189 37.0 37.0 37.0 37.0 37.0 95-99 36.04748781607113 37.0 37.0 37.0 37.0 37.0 100-104 35.98259853704663 37.0 37.0 37.0 37.0 37.0 105-109 35.9553707946353 37.0 37.0 37.0 37.0 37.0 110-114 35.83792007860359 37.0 37.0 37.0 37.0 37.0 115-119 35.93576942561502 37.0 37.0 37.0 37.0 37.0 120-124 35.84390257980389 37.0 37.0 37.0 37.0 37.0 125-129 35.83556497642637 37.0 37.0 37.0 37.0 37.0 130-134 35.84758839619269 37.0 37.0 37.0 37.0 37.0 135-139 35.846416392478716 37.0 37.0 37.0 37.0 37.0 140-144 35.83646208231555 37.0 37.0 37.0 37.0 37.0 145-149 35.73606729209895 37.0 37.0 37.0 37.0 37.0 150 35.78437220399642 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 4.0 14 1.0 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 1.0 21 0.0 22 5.0 23 8.0 24 13.0 25 8.0 26 5.0 27 6.0 28 9.0 29 12.0 30 20.0 31 28.0 32 40.0 33 77.0 34 136.0 35 441.0 36 2852.0 37 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.275 19.975 10.75 22.0 2 31.075000000000003 23.799999999999997 25.924999999999997 19.2 3 26.974999999999998 27.85 29.25 15.925 4 27.3 30.8 23.375 18.525 5 29.95 33.85 17.724999999999998 18.475 6 24.575 39.175 18.75 17.5 7 26.1 22.0 31.075000000000003 20.825 8 25.45 24.65 26.700000000000003 23.200000000000003 9 28.449999999999996 25.15 26.650000000000002 19.75 10-14 29.470000000000002 27.089999999999996 23.78 19.66 15-19 27.965 26.44 26.43 19.165 20-24 29.26 29.48 22.759999999999998 18.5 25-29 28.15 25.869999999999997 26.07 19.91 30-34 27.224999999999998 24.235 27.435 21.105 35-39 28.439999999999998 25.995 26.525 19.040000000000003 40-44 26.419999999999998 24.95 26.13 22.5 45-49 26.790000000000003 27.555000000000003 24.755 20.9 50-54 26.568985347802172 29.80947142071311 24.753713056958542 18.86783017452618 55-59 29.162290572643162 25.676419104776194 24.466116529132282 20.69517379344836 60-64 30.237559389847462 23.905976494123532 23.885971492873217 21.97049262315579 65-69 26.906726681670417 25.681420355088775 25.826456614153535 21.585396349087272 70-74 25.98149537384346 26.14653663415854 25.926481620405102 21.9454863715929 75-79 27.153576788394197 27.088544272136065 25.317658829414707 20.440220110055026 80-84 26.210726435861513 27.691614968981387 25.290174104462675 20.807484490694417 85-89 26.218992733650715 26.103733400150336 26.930593836131294 20.74668003006765 90-94 25.762064982674637 26.962285943855775 26.304424245467782 20.97122482800181 95-99 26.398063637738893 27.38137259845696 23.659926377893196 22.560637385910947 100-104 29.320142059868086 27.189244038559107 23.505834601725013 19.984779299847794 105-109 28.803266139321256 25.475886705792295 24.929829038019903 20.791018116866546 110-114 24.874929083500955 27.670328536799214 25.741399762752078 21.713342616947752 115-119 26.037518339970656 26.50387759379585 26.8339970656047 20.6246070006288 120-124 26.674819225861334 25.606125053168867 26.903445342407483 20.81561037856231 125-129 26.712735518004642 25.827349781352915 26.696539437456135 20.763375263186308 130-134 26.4286892910181 26.181178153016887 24.525603652164346 22.86452890380067 135-139 23.25344788605019 27.379606601853943 25.746099932172733 23.62084557992313 140-144 25.323517570598725 26.899407603381835 24.391786967274403 23.385287858745038 145-149 25.68661971830986 28.749999999999996 23.679577464788732 21.883802816901408 150 25.350432448553534 31.434536236206384 22.099612287503728 21.11541902773636 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 1.0 24 1.5 25 1.0 26 0.0 27 0.0 28 0.5 29 1.5 30 1.0 31 0.0 32 1.5 33 3.5 34 2.5 35 2.0 36 16.0 37 34.0 38 40.5 39 47.5 40 113.5 41 145.0 42 121.5 43 130.0 44 151.5 45 185.0 46 168.0 47 175.5 48 337.5 49 429.0 50 370.5 51 371.0 52 397.5 53 276.5 54 162.0 55 128.5 56 66.0 57 21.5 58 1.5 59 2.5 60 3.5 61 4.5 62 7.5 63 8.0 64 12.0 65 14.5 66 8.5 67 12.5 68 14.0 69 5.0 70 1.5 71 1.0 72 0.0 73 0.5 74 1.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 50-51 1.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0 84-85 5.0 86-87 2.0 88-89 5.0 90-91 0.0 92-93 6.0 94-95 10.0 96-97 6.0 98-99 8.0 100-101 15.0 102-103 7.0 104-105 9.0 106-107 8.0 108-109 15.0 110-111 23.0 112-113 24.0 114-115 29.0 116-117 18.0 118-119 27.0 120-121 20.0 122-123 22.0 124-125 24.0 126-127 22.0 128-129 19.0 130-131 27.0 132-133 59.0 134-135 34.0 136-137 28.0 138-139 24.0 140-141 21.0 142-143 34.0 144-145 29.0 146-147 18.0 148-149 46.0 150-151 3353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.075000000000003 #Duplication Level Percentage of deduplicated Percentage of total 1 43.66197183098591 10.075000000000001 2 15.276273022751896 7.049999999999999 3 8.992416034669557 6.225 4 7.042253521126761 6.5 5 3.9003250270855903 4.5 6 2.4918743228602382 3.45 7 2.925243770314193 4.725 8 2.275189599133261 4.2 9 1.6251354279523293 3.375 >10 11.592632719393283 45.35 >50 0.10834236186348861 1.55 >100 0.10834236186348861 3.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTT 120 3.0 No Hit GAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAA 62 1.55 No Hit GGGGAACCAGTGCTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGG 45 1.125 No Hit GGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGAAGA 44 1.0999999999999999 No Hit AATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAA 41 1.0250000000000001 No Hit CGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGAAG 38 0.95 No Hit GACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGA 38 0.95 No Hit GTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTC 37 0.9249999999999999 No Hit GTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACT 35 0.8750000000000001 No Hit GCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCA 35 0.8750000000000001 No Hit GGGAACCAGTGCTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGGA 34 0.8500000000000001 No Hit GGGGCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCC 28 0.7000000000000001 No Hit CGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGA 27 0.675 No Hit GGCAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGA 26 0.65 No Hit CTTTGAACAGCGCCGGCCTCAAATGCAGTTCCAGTTAGCTATTTTCACGC 26 0.65 No Hit AGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGACCG 26 0.65 No Hit GGGCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCT 25 0.625 No Hit GTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGACCGGCGCCTA 24 0.6 No Hit GCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGT 24 0.6 No Hit GGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATGCAGTTCCAGTTAG 23 0.575 No Hit AGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCG 23 0.575 No Hit GGAACCAGTGCTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGGAA 22 0.5499999999999999 No Hit GGTATGCCCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGC 22 0.5499999999999999 No Hit GAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGAC 21 0.525 No Hit GTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGG 21 0.525 No Hit GTATGCCCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCT 21 0.525 No Hit GAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAA 20 0.5 No Hit CTGGAGTCTACGAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTC 20 0.5 No Hit GTTGCTTTCACAGTTTATGAGGACTTTGCACACTACAAATCTGGAGTCTA 20 0.5 No Hit GTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATC 20 0.5 No Hit GGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAG 20 0.5 No Hit GCAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGAT 20 0.5 No Hit GTGGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCA 20 0.5 No Hit GTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGT 19 0.475 No Hit GAAACATTTCAGCATCAATGCATACAGAGTAAATTCTGATCCACATGACA 18 0.44999999999999996 No Hit GCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGATACTTCA 18 0.44999999999999996 No Hit GTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTATCCTTCCTTG 18 0.44999999999999996 No Hit GGATGGGGAACCAGTGCTGATGGCAAAGATTACTGGCTTCTTGCAAATCA 17 0.42500000000000004 No Hit TGGGGAACCAGTGCTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTG 16 0.4 No Hit CTTCCTTGTGAGTATGTATGTATCTGTGTGTCTGTGTGTGTAATTGGTAC 16 0.4 No Hit AGAAGTTGCTTTCACAGTTTATGAGGACTTTGCACACTACAAATCTGGAG 16 0.4 No Hit CAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGA 16 0.4 No Hit GGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGC 16 0.4 No Hit AGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGA 16 0.4 No Hit AATCAGTGGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAG 16 0.4 No Hit CTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGAAGATGTGG 16 0.4 No Hit CAGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATG 15 0.375 No Hit GTGCTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGC 15 0.375 No Hit GTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGT 15 0.375 No Hit AGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTGGA 15 0.375 No Hit GCTCATCATCTTGTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATT 15 0.375 No Hit GATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGACCGGCGC 15 0.375 No Hit GAGGACTTTGCACACTACAAATCTGGAGTCTACGAGCACATCACAGGTGA 15 0.375 No Hit CAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACA 14 0.35000000000000003 No Hit GCTGATTGGATGGGGAACCAGTGCTGATGGCAAAGATTACTGGCTTCTTG 14 0.35000000000000003 No Hit TGCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATCA 14 0.35000000000000003 No Hit TGTTAGTCCATTAATCATGATGAACTTGTGGTTAGTAAATAAGCGCCGAG 14 0.35000000000000003 No Hit GGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATGCAGT 14 0.35000000000000003 No Hit GAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGC 14 0.35000000000000003 No Hit AAATGATGGGAGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCT 14 0.35000000000000003 No Hit GCAGTACCTCGTTGAGAATGGTGTTGTTACTGACGAGTGCGATCCATACT 14 0.35000000000000003 No Hit AGATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCC 13 0.325 No Hit GCGCCGAGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTT 13 0.325 No Hit CTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTT 13 0.325 No Hit GGACTTTGCACACTACAAATCTGGAGTCTACGAGCACATCACAGGTGAAA 13 0.325 No Hit GGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGT 13 0.325 No Hit TGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCG 13 0.325 No Hit GCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTC 12 0.3 No Hit ACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGC 12 0.3 No Hit ACGAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATT 12 0.3 No Hit ATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATGCAGTTCCAGTT 12 0.3 No Hit CCAGTGCTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGA 12 0.3 No Hit AAGCGCCGAGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTC 12 0.3 No Hit GTCTACGAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCT 12 0.3 No Hit GCCCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATAC 12 0.3 No Hit CTACGAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGA 12 0.3 No Hit AATTGGTGTATCCTTCCTTGTGAGTATGTATGTATCTGTGTGTCTGTGTG 11 0.27499999999999997 No Hit GGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTG 11 0.27499999999999997 No Hit AGTAAATAAGCGCCGAGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTA 11 0.27499999999999997 No Hit TGTATCTGTGTGTCTGTGTGTGTAATTGGTACATTTGATCAAGCTCTCTG 11 0.27499999999999997 No Hit GACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATG 11 0.27499999999999997 No Hit TGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCT 11 0.27499999999999997 No Hit GCCGAGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAA 11 0.27499999999999997 No Hit GAACTTGTGGTTAGTAAATAAGCGCCGAGTCAGAGCGTGTGGTGTGATTG 11 0.27499999999999997 No Hit CGGAGGTCTACAAAAATGGCCCTGTAGAAGTTGCTTTCACAGTTTATGAG 11 0.27499999999999997 No Hit AGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACA 11 0.27499999999999997 No Hit GTAAATAAGCGCCGAGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTAC 11 0.27499999999999997 No Hit TGTATCCTTCCTTGTGAGTATGTATGTATCTGTGTGTCTGTGTGTGTAAT 11 0.27499999999999997 No Hit AGCTGATTGGATGGGGAACCAGTGCTGATGGCAAAGATTACTGGCTTCTT 11 0.27499999999999997 No Hit GGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAG 11 0.27499999999999997 No Hit GAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGACCGG 11 0.27499999999999997 No Hit ATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAG 11 0.27499999999999997 No Hit GTAGAAGTTGCTTTCACAGTTTATGAGGACTTTGCACACTACAAATCTGG 11 0.27499999999999997 No Hit CTTGTAACTGTTAGTCCATTAATCATGATGAACTTGTGGTTAGTAAATAA 10 0.25 No Hit AACAGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAA 10 0.25 No Hit CGAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTG 10 0.25 No Hit CTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGG 10 0.25 No Hit GCTGCGGCGACAACTGCAAGTGCAACCCTTGTAACTGTTAGTCCATTAAT 10 0.25 No Hit AAATAAGCGCCGAGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTT 10 0.25 No Hit AATGGCGGAGGTCTACAAAAATGGCCCTGTAGAAGTTGCTTTCACAGTTT 10 0.25 No Hit GGCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTC 10 0.25 No Hit TGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGA 10 0.25 No Hit GGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCAT 10 0.25 No Hit ATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCGAA 10 0.25 No Hit CATCAATGCATACAGAGTAAATTCTGATCCACATGACATAATGGCGGAGG 10 0.25 No Hit GGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAAT 10 0.25 No Hit GTAAATTCTGATCCACATGACATAATGGCGGAGGTCTACAAAAATGGCCC 10 0.25 No Hit CAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGATG 10 0.25 No Hit GTATGTATCTGTGTGTCTGTGTGTGTAATTGGTACATTTGATCAAGCTCT 10 0.25 No Hit TGTAGAAGTTGCTTTCACAGTTTATGAGGACTTTGCACACTACAAATCTG 9 0.22499999999999998 No Hit GTATGTATGTATCTGTGTGTCTGTGTGTGTAATTGGTACATTTGATCAAG 9 0.22499999999999998 No Hit GCTATACTTTGAACAGCGCCGGCCTCAAATGCAGTTCCAGTTAGCTATTT 9 0.22499999999999998 No Hit GGCGGAGGTCTACAAAAATGGCCCTGTAGAAGTTGCTTTCACAGTTTATG 9 0.22499999999999998 No Hit TGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAGGACCGGCGCCT 9 0.22499999999999998 No Hit ATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCTGATGGCAAAGATTAC 9 0.22499999999999998 No Hit GTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTATCCTT 9 0.22499999999999998 No Hit ATTCTGATCCACATGACATAATGGCGGAGGTCTACAAAAATGGCCCTGTA 9 0.22499999999999998 No Hit CTACAAATCTGGAGTCTACGAGCACATCACAGGTGAAATGATGGGAGGTC 9 0.22499999999999998 No Hit AGTGGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGC 9 0.22499999999999998 No Hit GAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAG 9 0.22499999999999998 No Hit GAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTAT 9 0.22499999999999998 No Hit GTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTATCCTTCC 9 0.22499999999999998 No Hit TGTATGTATCTGTGTGTCTGTGTGTGTAATTGGTACATTTGATCAAGCTC 9 0.22499999999999998 No Hit CAGGGCCTGGTTCCCGACCATCAAACAGAGTGGCTTGGTGTAGACAATTG 9 0.22499999999999998 No Hit GTGTGTCTGTGTGTGTAATTGGTACATTTGATCAAGCTCTCTGCACTGGG 8 0.2 No Hit CAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAA 8 0.2 No Hit GCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATGCAGTT 8 0.2 No Hit AGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAAC 8 0.2 No Hit CATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCA 8 0.2 No Hit TCTACGAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTG 8 0.2 No Hit CCTTCCTTGTGAGTATGTATGTATCTGTGTGTCTGTGTGTGTAATTGGTA 8 0.2 No Hit CTTTGATCAGGTTGGCTGCAAGCATCCTGGATGCGAACCTGCTTATCCTA 8 0.2 No Hit CCTCAAATGCAGTTCCAGTTAGCTATTTTCACGCCAGATGAAGTGGGTAA 8 0.2 No Hit CAAATCTGGAGTCTACGAGCACATCACAGGTGAAATGATGGGAGGTCATG 8 0.2 No Hit GCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATCAT 8 0.2 No Hit GTGTTGTTACTGACGAGTGCGATCCATACTTTGATCAGGTTGGCTGCAAG 8 0.2 No Hit GCGCCGGCCTCAAATGCAGTTCCAGTTAGCTATTTTCACGCCAGATGAAG 8 0.2 No Hit AACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAA 8 0.2 No Hit GCTTATCCTACACCAGCATGTGAAAAGAAATGCAAGGTGCAGAACCAAGT 8 0.2 No Hit CCCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACT 8 0.2 No Hit GTGTAATTGGTACATTTGATCAAGCTCTCTGCACTGGGGAGTTTGGCCAG 8 0.2 No Hit GACAACTGCAAGTGCAACCCTTGTAACTGTTAGTCCATTAATCATGATGA 8 0.2 No Hit GAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTGG 8 0.2 No Hit GTTCGAGATGGCCGGCGTGTCCGGCGAAGGGTGCAGCTGCGGCGACAACT 8 0.2 No Hit GATGAACTTGTGGTTAGTAAATAAGCGCCGAGTCAGAGCGTGTGGTGTGA 8 0.2 No Hit AATGATGGGAGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCTG 7 0.17500000000000002 No Hit GCCATGGGGACCCTTTGGGAGGGTTGTCTTTGCACACACTTTTATGGATC 7 0.17500000000000002 No Hit CCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATGCAGTTCCA 7 0.17500000000000002 No Hit ATCGAAGAAGATGTGGTTGCCGGTATGCCCTCAACAAAGAACACTGTCAG 7 0.17500000000000002 No Hit GGAAGATGTACCCAGACCTGGCAGAGCAGGCCAGCACCACCAGCAGCACC 7 0.17500000000000002 No Hit TGTAACTGTTAGTCCATTAATCATGATGAACTTGTGGTTAGTAAATAAGC 7 0.17500000000000002 No Hit ATAAGCGCCGAGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGC 7 0.17500000000000002 No Hit TGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTATCCTTCCTTGT 7 0.17500000000000002 No Hit CAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAA 7 0.17500000000000002 No Hit AAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAG 7 0.17500000000000002 No Hit ATAAGGGGCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTAT 7 0.17500000000000002 No Hit CTGTCAGGACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCC 7 0.17500000000000002 No Hit ATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTGGATGGGG 7 0.17500000000000002 No Hit AAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGATGA 7 0.17500000000000002 No Hit CATGGGGACCCTTTGGGAGGGTTGTCTTTGCACACACTTTTATGGATCGT 7 0.17500000000000002 No Hit CACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTGGATGGGGAA 7 0.17500000000000002 No Hit CCTTGATACAAGGAGAAGAACTCCCAAATTGGTTCACTCTGTTGCTACCA 7 0.17500000000000002 No Hit TGATGGGAGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCTGAT 7 0.17500000000000002 No Hit CGGTATGCCCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGG 7 0.17500000000000002 No Hit ACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGAA 7 0.17500000000000002 No Hit CTTTGCACACTACAAATCTGGAGTCTACGAGCACATCACAGGTGAAATGA 7 0.17500000000000002 No Hit GTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTATCCTTCCTTGTG 7 0.17500000000000002 No Hit TCATCATCTTGTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATTTC 7 0.17500000000000002 No Hit GTCTACAAAAATGGCCCTGTAGAAGTTGCTTTCACAGTTTATGAGGACTT 7 0.17500000000000002 No Hit GTGTATCCTTCCTTGTGAGTATGTATGTATCTGTGTGTCTGTGTGTGTAA 7 0.17500000000000002 No Hit CAGTGGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATCATAAGGGG 7 0.17500000000000002 No Hit TCCCATAAGATCTACTGTGACAAAGGCTGTTGCTGAGTTTAAGAGGACAC 7 0.17500000000000002 No Hit TATACTTTGAACAGCGCCGGCCTCAAATGCAGTTCCAGTTAGCTATTTTC 6 0.15 No Hit ACATAATGGCGGAGGTCTACAAAAATGGCCCTGTAGAAGTTGCTTTCACA 6 0.15 No Hit GAGTATGTATGTATCTGTGTGTCTGTGTGTGTAATTGGTACATTTGATCA 6 0.15 No Hit AAGGTAAGCATCGTAGGACCCAATAACGGTCCTGTATCAGCAACATCGGT 6 0.15 No Hit AGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTATC 6 0.15 No Hit AAGGGGCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCGGTATGC 6 0.15 No Hit CTGGGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTG 6 0.15 No Hit GTCAAGCTGATTGGATGGGGAACCAGTGCTGATGGCAAAGATTACTGGCT 6 0.15 No Hit ATTTCCCGCAGATGCTCAGGGCCTGGTTCCCGACCATCAAACAGAGTGGC 6 0.15 No Hit GTGTTGTTGTTTACTTGCTCTTAATTGGTGTATCCTTCCTTGTGAGTATG 6 0.15 No Hit CCAAGTTTGGCAGGAAAAGAAACATTTCAGCATCAATGCATACAGAGTAA 6 0.15 No Hit GTTGAAGCAGAGCAATTCCTTACACACTCTTTTGTTGATCCAGACCTTGA 6 0.15 No Hit AGGTCTACAAAAATGGCCCTGTAGAAGTTGCTTTCACAGTTTATGAGGAC 6 0.15 No Hit GGGTGATGACGGATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCA 6 0.15 No Hit TATGCCCTCAACAAAGAACACTGTCAGGACCGGCGCCTATGGAACGGCTA 6 0.15 No Hit ATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGA 6 0.15 No Hit CTGTGTGTCTGTGTGTGTAATTGGTACATTTGATCAAGCTCTCTGCACTG 6 0.15 No Hit CTGTGTGTGTAATTGGTACATTTGATCAAGCTCTCTGCACTGGGGAGTTT 6 0.15 No Hit CATAACTGGGACAAATCTGAGAATGAAAGAACCGCGTGTTTCTATGAGTA 6 0.15 No Hit TATGTATCTGTGTGTCTGTGTGTGTAATTGGTACATTTGATCAAGCTCTC 6 0.15 No Hit GTTGTGTTAAGCCTACTGGGTGGCATAACTGGGACAAATCTGAGAATGAA 6 0.15 No Hit GATACTTCAAGATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGAAGAT 6 0.15 No Hit GGGAGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCTGATGGCA 6 0.15 No Hit GCGTGTCCGGCGAAGGGTGCAGCTGCGGCGACAACTGCAAGTGCAACCCT 5 0.125 No Hit GCCTCAAATGCAGTTCCAGTTAGCTATTTTCACGCCAGATGAAGTGGGTA 5 0.125 No Hit TTCTGATCCACATGACATAATGGCGGAGGTCTACAAAAATGGCCCTGTAG 5 0.125 No Hit ACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGATAC 5 0.125 No Hit TTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGAT 5 0.125 No Hit TGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGTATCCTTCCTTGTGA 5 0.125 No Hit GGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCTGATGGCAAAGA 5 0.125 No Hit AGTCAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGG 5 0.125 No Hit GTGTGTAATTGGTACATTTGATCAAGCTCTCTGCACTGGGGAGTTTGGCC 5 0.125 No Hit ACCAGCAGCACCCAGGCCCAGGTGCTGGTTCTCGGCATGGCGCCGGAGAA 5 0.125 No Hit GTGAAAAGAAATGCAAGGTGCAGAACCAAGTTTGGCAGGAAAAGAAACAT 5 0.125 No Hit GATCATAAGGGGCAAGAATGAGTGTGGCATCGAAGAAGATGTGGTTGCCG 5 0.125 No Hit GGTGCAGAACCAAGTTTGGCAGGAAAAGAAACATTTCAGCATCAATGCAT 5 0.125 No Hit GCTGATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTG 5 0.125 No Hit AATTGGTACATTTGATCAAGCTCTCTGCACTGGGGAGTTTGGCCAGTGAA 5 0.125 No Hit GGAGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCTGATGGCAA 5 0.125 No Hit CAAAAATGGCCCTGTAGAAGTTGCTTTCACAGTTTATGAGGACTTTGCAC 5 0.125 No Hit ACCGGCGCCTATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATGC 5 0.125 No Hit GCGATCCATACTTTGATCAGGTTGGCTGCAAGCATCCTGGATGCGAACCT 5 0.125 No Hit AAGCTGATTGGATGGGGAACCAGTGCTGATGGCAAAGATTACTGGCTTCT 5 0.125 No Hit CCTTCGGTGCTGTGGAGTGTCTCCAGGATCGTTTCTGCATTCATCTTAAC 5 0.125 No Hit CTTGGAGCATCCAGAAGGATGCCTTCACAGAAGATGAATTGGAGGTTCTC 5 0.125 No Hit TATGGAACGGCTATACTTTGAACAGCGCCGGCCTCAAATGCAGTTCCAGT 5 0.125 No Hit CTTGTGGTTAGTAAATAAGCGCCGAGTCAGAGCGTGTGGTGTGATTGTGT 5 0.125 No Hit AGACAATTGCTTGATGTTGAAGCAGAGCAATTCCTTACACACTCTTTTGT 5 0.125 No Hit TTGCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGATACTTCAAGATC 5 0.125 No Hit CACTGCTTGCCCGCTTCATCCGTGAGCCGTCTCCCATAAGATCTACTGTG 5 0.125 No Hit CACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAGCTGATTGGATG 5 0.125 No Hit CTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGGTGATGACGGATACT 5 0.125 No Hit AGGTCATGCTGTCAAGCTGATTGGATGGGGAACCAGTGCTGATGGCAAAG 5 0.125 No Hit GGCCTCAAATGCAGTTCCAGTTAGCTATTTTCACGCCAGATGAAGTGGGT 5 0.125 No Hit CAGAGCGTGTGGTGTGATTGTGTTGTTGTTTACTTGCTCTTAATTGGTGT 5 0.125 No Hit GATGGCAAAGATTACTGGCTTCTTGCAAATCAGTGGAACAGAGGCTGGGG 5 0.125 No Hit GAGCAGGCCAGCACCACCAGCAGCACCCAGGCCCAGGTGCTGGTTCTCGG 5 0.125 No Hit GAGTCTACGAGCACATCACAGGTGAAATGATGGGAGGTCATGCTGTCAAG 5 0.125 No Hit AACCAAGCATATTCAGTATGCCACATCACTAACCATTTTATCACTATTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601088 spots for ERR5262797.sra Written 1601088 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra Read 1601073 spots for ERR5262797.sra Written 1601073 spots for ERR5262797.sra SRR ids: ['ERR5262797.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd___ttnczr ERR5262797.sra spots: 32021475 blocks: [[1, 1601073], [1601074, 3202146], [3202147, 4803219], [4803220, 6404292], [6404293, 8005365], [8005366, 9606438], [9606439, 11207511], [11207512, 12808584], [12808585, 14409657], [14409658, 16010730], [16010731, 17611803], [17611804, 19212876], [19212877, 20813949], [20813950, 22415022], [22415023, 24016095], [24016096, 25617168], [25617169, 27218241], [27218242, 28819314], [28819315, 30420387], [30420388, 32021475]] ERR5262797 file size 10462623 ERR5262797 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262797 ERR5262797_1.fastq ERR5262797_2.fastq Input file: ERR5262797_1.fastq Paired file: ERR5262797_2.fastq trimmed: ERR5262797-trimmed-pair1.fastq, ERR5262797-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:59:00 2024 >> started Fri Dec 6 11:59:35 2024 >> done (34.815s) 32021475 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 2 ( 0.00%) empty read pairs filtered out after trimming by size control 32021473 (100.00%) read pairs available; of these: 10628 ( 0.03%) trimmed read pairs available after processing 32010845 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 2 0.00% 22 0 0.00% 23 0 0.00% 24 1 0.00% 25 0 0.00% 26 1 0.00% 27 0 0.00% 28 0 0.00% 29 1 0.00% 30 1 0.00% 31 1 0.00% 32 1 0.00% 33 2 0.00% 34 2 0.00% 35 0 0.00% 36 2 0.00% 37 2 0.00% 38 7 0.00% 39 7 0.00% 40 0 0.00% 41 1 0.00% 42 1 0.00% 43 2 0.00% 44 3 0.00% 45 2 0.00% 46 5 0.00% 47 4 0.00% 48 1 0.00% 49 369 0.00% 50 438 0.00% 51 468 0.00% 52 488 0.00% 53 543 0.00% 54 632 0.00% 55 584 0.00% 56 752 0.00% 57 840 0.00% 58 911 0.00% 59 1093 0.00% 60 1250 0.00% 61 1464 0.00% 62 1704 0.01% 63 1797 0.01% 64 2055 0.01% 65 2112 0.01% 66 2385 0.01% 67 2695 0.01% 68 3008 0.01% 69 3462 0.01% 70 4138 0.01% 71 4798 0.01% 72 5382 0.02% 73 6195 0.02% 74 6749 0.02% 75 7421 0.02% 76 8204 0.03% 77 9138 0.03% 78 9644 0.03% 79 10825 0.03% 80 12115 0.04% 81 13699 0.04% 82 15534 0.05% 83 17649 0.06% 84 19249 0.06% 85 21464 0.07% 86 22007 0.07% 87 24023 0.08% 88 25310 0.08% 89 26719 0.08% 90 28937 0.09% 91 31900 0.10% 92 34580 0.11% 93 37825 0.12% 94 40424 0.13% 95 42241 0.13% 96 44470 0.14% 97 46260 0.14% 98 47516 0.15% 99 49381 0.15% 100 51647 0.16% 101 53756 0.17% 102 57669 0.18% 103 60765 0.19% 104 64062 0.20% 105 66788 0.21% 106 68975 0.22% 107 70452 0.22% 108 71570 0.22% 109 73559 0.23% 110 74143 0.23% 111 77194 0.24% 112 80801 0.25% 113 83394 0.26% 114 86743 0.27% 115 89842 0.28% 116 90382 0.28% 117 93038 0.29% 118 94007 0.29% 119 94880 0.30% 120 95907 0.30% 121 98505 0.31% 122 99655 0.31% 123 102924 0.32% 124 108025 0.34% 125 107759 0.34% 126 110518 0.35% 127 111452 0.35% 128 112092 0.35% 129 113425 0.35% 130 114475 0.36% 131 114455 0.36% 132 116400 0.36% 133 119206 0.37% 134 120796 0.38% 135 123191 0.38% 136 126638 0.40% 137 126154 0.39% 138 127337 0.40% 139 129675 0.40% 140 129402 0.40% 141 133143 0.42% 142 135543 0.42% 143 135490 0.42% 144 140007 0.44% 145 141834 0.44% 146 144411 0.45% 147 276120 0.86% 148 133691 0.42% 149 131933 0.41% 150 26034747 81.30% 32021473 reads passed initial QC criterion=sequence-density sequence-density=1.99 sequence-density-rank=1 fanout-score=2.40 fanout-score-rank=25 prefix-density=2.22 prefix-fanout=2.1 sequence=CCAGTCTCCCTGTCCTGGATGATCTTGGCGTCGAGGATCTCGCCGAAGTTGCTGAAGGCGGACTGGAGGTTGTGGTCGTCGGTGGCCCAGGCGAGGCCGCCCACGAAGCAGCGGTACTCATCCATGGCGATCTCGACGGAACCCTACCGGAAACCCTAGCTGCTACTACAACAACTACTGCTAGCCGCGAACCGGAACCCAGGACCCGAACCGAACCGAAC criterion=fanout-score sequence-density=0.04 sequence-density-rank=25 fanout-score=127.15 fanout-score-rank=1 prefix-density=0.94 prefix-fanout=5.2 sequence=CGCCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGC criterion=sequence-density sequence-density=2.01 sequence-density-rank=1 fanout-score=2.04 fanout-score-rank=25 prefix-density=2.02 prefix-fanout=2.0 sequence=TGCTTCGTGGGCGGCCTCGCCTGGGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=25 fanout-score=78.11 fanout-score-rank=1 prefix-density=0.62 prefix-fanout=4.0 sequence=AGCTCAAGGAGAAGTTTGAGGGTCTCTGCAAGGTTATCAAGGAGGTGCTCGGCGACAGGGTGGAGAAGGTCATTGTCTCTGACCGTGTTGTTGACTCGCCGTGCTGCCTTGTCACTGGTGAGTATGGATGGACTGCCAACATGGAGAGGATCATGAAGGCCCAGGCCCTGAGGGACTCTAGCATGGCTGGTTACATGTCCAGCAAGAAGACCATGGAGATCAACCCGGAGAATGCCATCATGGAGGAGCTGCGCAAGCGGGCTGATGCTGACAAGAACGACAAGTCAGTCAAGGACCTGGTGATGCTCTTGTTTGAGACCTCCCTGCTCACATCTGGGTTCAGCCTGGACGACCCCAACACCTTCGGCACCAGGATCCACCGCATGCTCAAGCTTGGCCTTAGCATCGATGAGGACGAGACTGCCGAGGCTGATGACACAGATATGCCCGCGCTGGAGGATGATGCCGGCGAGAGCAAGATGGAGGAGGTCGACTAAGTCTATTATCGTTA Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x CCAGTCTCCCTGTCCTGGATGATCTTGGCGTCGAGGATCTCGCCGAAGTTGCTGAAGGCGGACTGGAGGTTGTGGTCGTCGGTGGCCCAGGCGAGGCCGCCCACGAAGCAGCGGTACTCATCCATGGCGATCTCGACGGAACCCTACCGGAAACCCTAGCTGCTACTACAACAACTACTGCTAGCCGCGAACCGGAACCCAGGACCCGAACCGAACCGAAC -y TGCTTCGTGGGCGGCCTCGCCTGGGC -o ERR5262797 ERR5262797_1.fastq ERR5262797_2.fastq Input file: ERR5262797_1.fastq Paired file: ERR5262797_2.fastq trimmed: ERR5262797-trimmed-pair1.fastq, ERR5262797-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): CCAGTCTCCCTGTCCTGGATGATCTTGGCGTCGAGGATCTCGCCGAAGTTGCTGAAGGCGGACT -- paired 3' end adapter sequence (-y): TGCTTCGTGGGCGGCCTCGCCTGGGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:03:50 2024 >> started Fri Dec 6 12:04:02 2024 >> done (12.357s) 10673824 read pairs processed; of these: 184 ( 0.00%) short read pairs filtered out after trimming by size control 134 ( 0.00%) empty read pairs filtered out after trimming by size control 10673506 (100.00%) read pairs available; of these: 86 ( 0.00%) trimmed read pairs available after processing 10673420 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 31 1 0.00% 32 0 0.00% 33 0 0.00% 34 1 0.00% 35 0 0.00% 36 0 0.00% 37 0 0.00% 38 2 0.00% 39 1 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 0 0.00% 44 1 0.00% 45 0 0.00% 46 2 0.00% 47 2 0.00% 48 0 0.00% 49 124 0.00% 50 155 0.00% 51 238 0.00% 52 257 0.00% 53 282 0.00% 54 324 0.00% 55 287 0.00% 56 389 0.00% 57 405 0.00% 58 398 0.00% 59 334 0.00% 60 471 0.00% 61 472 0.00% 62 539 0.01% 63 596 0.01% 64 586 0.01% 65 706 0.01% 66 621 0.01% 67 838 0.01% 68 913 0.01% 69 1170 0.01% 70 1914 0.02% 71 2208 0.02% 72 1633 0.02% 73 2020 0.02% 74 1770 0.02% 75 1728 0.02% 76 2267 0.02% 77 3692 0.03% 78 3328 0.03% 79 3844 0.04% 80 5007 0.05% 81 5961 0.06% 82 5462 0.05% 83 4405 0.04% 84 6911 0.06% 85 5883 0.06% 86 8136 0.08% 87 9348 0.09% 88 9334 0.09% 89 7445 0.07% 90 9443 0.09% 91 9741 0.09% 92 11616 0.11% 93 11699 0.11% 94 13740 0.13% 95 14402 0.13% 96 14422 0.14% 97 16133 0.15% 98 15941 0.15% 99 15757 0.15% 100 17877 0.17% 101 19488 0.18% 102 17411 0.16% 103 18878 0.18% 104 20773 0.19% 105 21959 0.21% 106 21606 0.20% 107 23878 0.22% 108 23015 0.22% 109 24035 0.23% 110 26126 0.24% 111 24940 0.23% 112 28165 0.26% 113 26727 0.25% 114 28788 0.27% 115 29460 0.28% 116 30824 0.29% 117 30996 0.29% 118 29820 0.28% 119 32458 0.30% 120 31435 0.29% 121 34206 0.32% 122 33614 0.31% 123 34243 0.32% 124 35997 0.34% 125 35394 0.33% 126 36721 0.34% 127 36552 0.34% 128 37635 0.35% 129 37900 0.36% 130 38043 0.36% 131 38119 0.36% 132 38270 0.36% 133 39970 0.37% 134 40169 0.38% 135 40936 0.38% 136 42005 0.39% 137 41828 0.39% 138 42543 0.40% 139 43181 0.40% 140 43180 0.40% 141 43883 0.41% 142 45201 0.42% 143 44954 0.42% 144 46805 0.44% 145 47140 0.44% 146 48386 0.45% 147 92995 0.87% 148 44626 0.42% 149 44261 0.41% 150 8680785 81.33% criterion=sequence-density sequence-density=0.15 sequence-density-rank=1 fanout-score=3.99 fanout-score-rank=40 prefix-density=0.08 prefix-fanout=4.0 sequence=ACACACACACAAGCCGAGAACAGGCAGTAATAAGCCAAGCCACCGCCGCCCTTTTCTTCCATCGATCTCTCCGGCGTAAGATGAAGCTATCATTGAATTAGCCGCCGCGGCCGGCAACTGTCGAGACGAGAGAGCGCGCGCGGGAACGTCGGTAGCGGCAGACCACAACAAACCTCCTTCCATCGTCCCATCTGATCTCCCGGATACTCATGGCGCGCGCTGGACGAGAGTAGAGGTCGAGGTCCGCATGGGGGGCCCTCTCAAGCC criterion=fanout-score sequence-density=0.10 sequence-density-rank=11 fanout-score=192.29 fanout-score-rank=1 prefix-density=0.87 prefix-fanout=23.0 sequence=CAGCAGCAGCAG criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=38 prefix-density=0.59 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=23 fanout-score=239.78 fanout-score-rank=1 prefix-density=0.82 prefix-fanout=24.7 sequence=GCCGCCGCCGCAG ERR5262797 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:05:05 Started mapping on | Dec 06 12:05:05 Finished on | Dec 06 12:07:27 Mapping speed, Million of reads per hour | 811.80 Number of input reads | 32021155 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 30920547 Uniquely mapped reads % | 96.56% Average mapped length | 289.49 Number of splices: Total | 26310917 Number of splices: Annotated (sjdb) | 24390661 Number of splices: GT/AG | 25951322 Number of splices: GC/AG | 309989 Number of splices: AT/AC | 17800 Number of splices: Non-canonical | 31806 Mismatch rate per base, % | 0.11% Deletion rate per base | 0.00% Deletion average length | 1.51 Insertion rate per base | 0.00% Insertion average length | 1.13 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 269819 % of reads mapped to multiple loci | 0.84% Number of reads mapped to too many loci | 1183 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.58% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 830789 830789 830789 N_multimapping 269819 269819 269819 N_noFeature 1250062 30022270 1548215 N_ambiguous 685116 4252 85254 UnstrandedReadsAssigned:28985369 PositiveStrandReadsAssigned:894025 NegativeStrandReadsAssigned:29287078 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262797 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262797-trimmed-pair1.fastq ERR5262797-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,021,155 reads, 29,701,500 reads pseudoaligned [quant] estimated average fragment length: 242.104 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,200 rounds 52973 ERR5262797.ke.tsv 35125 ERR5262797.se.tsv 88098 total ==> ERR5262797.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 695.186 0 0 PNS24247 1044 802.896 167.274 10.0281 PNS24249 1928 1686.9 473.143 13.5006 PNS24246 1044 802.896 167.274 10.0281 PNS24248 1044 802.896 167.274 10.0281 PNS24244 1471 1229.9 271.034 10.6073 PNS24243 293 106.908 2 0.900474 KQK14069 1603 1361.9 55356.7 1956.48 KQK14071 474 251.47 112.661 21.5643 ==> ERR5262797.se.tsv <== BRADI_1g14170v3 55148 BRADI_1g53295v3 293 BRADI_1g59795v3 416 BRADI_1g07683v3 0 BRADI_1g00485v3 31 BRADI_1g20270v3 1077 BRADI_1g74790v3 2821 BRADI_1g09890v3 0 BRADI_1g77505v3 274 BRADI_1g48960v3 0 ERR5262797 completed mapping pipeline successfully