Starting /dee2/code/volunteer_pipeline.sh ERR5262798 current disk space = 1516051394560 free memory = 1570824748 ERR5262798 SRAfilesize 62996641feb34dfb219720eaf36a85f3 ERR5262798.sra ERR5262798.sra file validated ERR5262798 is paired end ERR5262798 is conventional basespace ERR5262798 read1 length is 79-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262798_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 79-150 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6935 37.0 37.0 37.0 37.0 37.0 2 36.396 37.0 37.0 37.0 37.0 37.0 3 36.56 37.0 37.0 37.0 37.0 37.0 4 36.5985 37.0 37.0 37.0 37.0 37.0 5 36.5745 37.0 37.0 37.0 37.0 37.0 6 36.685 37.0 37.0 37.0 37.0 37.0 7 36.606 37.0 37.0 37.0 37.0 37.0 8 36.598 37.0 37.0 37.0 37.0 37.0 9 36.635 37.0 37.0 37.0 37.0 37.0 10-14 36.525099999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.5148 37.0 37.0 37.0 37.0 37.0 20-24 36.3275 37.0 37.0 37.0 37.0 37.0 25-29 36.3389 37.0 37.0 37.0 37.0 37.0 30-34 36.3133 37.0 37.0 37.0 37.0 37.0 35-39 36.206100000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.1809 37.0 37.0 37.0 37.0 37.0 45-49 36.1652 37.0 37.0 37.0 37.0 37.0 50-54 36.1679 37.0 37.0 37.0 37.0 37.0 55-59 36.053200000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.0612 37.0 37.0 37.0 37.0 37.0 65-69 35.9501 37.0 37.0 37.0 37.0 37.0 70-74 35.9561 37.0 37.0 37.0 37.0 37.0 75-79 35.8979 37.0 37.0 37.0 37.0 37.0 80-84 35.970042510627664 37.0 37.0 37.0 37.0 37.0 85-89 35.87231605097068 37.0 37.0 37.0 37.0 37.0 90-94 35.77976965448172 37.0 37.0 37.0 37.0 37.0 95-99 35.78736807016843 37.0 37.0 37.0 37.0 37.0 100-104 35.74702268246482 37.0 37.0 37.0 37.0 37.0 105-109 35.82928713411519 37.0 37.0 37.0 37.0 37.0 110-114 35.69218057899864 37.0 37.0 37.0 37.0 37.0 115-119 35.73106959855926 37.0 37.0 37.0 37.0 37.0 120-124 35.76979744197463 37.0 37.0 37.0 37.0 37.0 125-129 35.680381806453326 37.0 37.0 37.0 37.0 37.0 130-134 35.60905039958159 37.0 37.0 37.0 37.0 37.0 135-139 35.567260909739794 37.0 37.0 37.0 37.0 37.0 140-144 35.42894978873441 37.0 37.0 37.0 37.0 37.0 145-149 35.4584853167339 37.0 37.0 37.0 37.0 37.0 150 35.368134855899946 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 3.0 23 3.0 24 2.0 25 3.0 26 6.0 27 9.0 28 12.0 29 30.0 30 28.0 31 63.0 32 95.0 33 118.0 34 194.0 35 402.0 36 2732.0 37 299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.05 12.375 9.775 28.799999999999997 2 23.736868434217108 9.929964982491246 36.01800900450225 30.315157578789393 3 20.325 18.5 33.225 27.950000000000003 4 21.725 26.974999999999998 28.425 22.875 5 20.424999999999997 33.4 26.224999999999998 19.950000000000003 6 22.85 37.974999999999994 25.35 13.825000000000001 7 15.725 26.700000000000003 40.975 16.6 8 15.174999999999999 28.275 33.5 23.05 9 18.9 26.474999999999998 34.925 19.7 10-14 20.015 27.169999999999998 29.23 23.585 15-19 20.04 25.905 30.264999999999997 23.79 20-24 19.84 27.195000000000004 28.845 24.12 25-29 21.52 27.665 28.139999999999997 22.675 30-34 18.490000000000002 24.73 30.654999999999998 26.125 35-39 19.985 27.689999999999998 28.84 23.485 40-44 19.994999999999997 28.749999999999996 30.740000000000002 20.515 45-49 17.895 25.445 32.54 24.12 50-54 23.200000000000003 25.805 29.985 21.01 55-59 20.080000000000002 31.259999999999998 26.490000000000002 22.17 60-64 17.145 32.545 29.709999999999997 20.599999999999998 65-69 19.470000000000002 31.215 28.735 20.580000000000002 70-74 18.05 32.015 27.055 22.88 75-79 17.585 34.195 25.955000000000002 22.264999999999997 80-84 18.0545136284071 30.977744436109028 29.382345586396596 21.585396349087272 85-89 18.403802852139105 31.833875406554917 27.175381536152116 22.586940205153866 90-94 20.105157736604905 32.353530295443164 27.346019028542813 20.19529293940911 95-99 18.913097921362386 32.19634360130228 29.391435011269724 19.499123466065615 100-104 20.785671282360024 29.876580373269114 27.543648404575556 21.794099939795302 105-109 22.656407161536915 32.141420237376785 26.04606718970026 19.15610541138604 110-114 21.42496974586527 29.074223477208548 30.758370310609116 18.742436466317063 115-119 22.095498783454985 27.559813463098138 28.533049472830495 21.811638280616382 120-124 21.51008762991645 29.19808436926839 26.33482779702466 22.957000203790503 125-129 22.47196764118581 27.17218780400389 29.025651528339562 21.330193026470738 130-134 26.244250348855235 24.631763915447827 27.24688614398677 21.877099591710166 135-139 22.10460447410961 26.145903947437034 28.080513114668616 23.668978463784743 140-144 22.41306415812282 26.561674241623507 27.05845048092168 23.966811119331993 145-149 21.177357677600043 29.16464695427371 26.046211019550814 23.61178434857543 150 19.73898858075041 36.56878738444807 24.089178901576943 19.60304513322458 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 0.5 16 0.5 17 0.5 18 0.5 19 0.0 20 0.5 21 0.5 22 0.0 23 0.5 24 1.0 25 1.5 26 4.5 27 6.5 28 10.5 29 10.0 30 9.0 31 67.0 32 71.0 33 35.5 34 56.0 35 76.0 36 113.5 37 158.5 38 160.0 39 163.5 40 222.5 41 310.0 42 327.0 43 414.5 44 550.5 45 424.0 46 215.0 47 157.0 48 112.0 49 62.5 50 81.5 51 58.0 52 16.0 53 7.0 54 3.5 55 2.5 56 1.0 57 1.5 58 1.5 59 0.5 60 23.0 61 22.5 62 0.0 63 1.0 64 1.0 65 0.0 66 0.0 67 0.0 68 1.0 69 3.5 70 4.5 71 3.0 72 5.5 73 6.0 74 1.5 75 2.0 76 2.5 77 2.5 78 2.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 78-79 1.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 5.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 0.0 98-99 1.0 100-101 8.0 102-103 2.0 104-105 2.0 106-107 6.0 108-109 2.0 110-111 6.0 112-113 3.0 114-115 15.0 116-117 8.0 118-119 7.0 120-121 9.0 122-123 3.0 124-125 9.0 126-127 14.0 128-129 12.0 130-131 13.0 132-133 23.0 134-135 9.0 136-137 8.0 138-139 19.0 140-141 27.0 142-143 39.0 144-145 23.0 146-147 20.0 148-149 27.0 150-151 3678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.45 #Duplication Level Percentage of deduplicated Percentage of total 1 40.191897654584224 9.425 2 18.44349680170576 8.649999999999999 3 10.341151385927505 7.2749999999999995 4 6.183368869936034 5.800000000000001 5 5.0106609808102345 5.875 6 5.0106609808102345 7.049999999999999 7 2.771855010660981 4.55 8 1.279317697228145 2.4 9 0.9594882729211088 2.025 >10 9.381663113006397 40.475 >50 0.42643923240938164 6.4750000000000005 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 84 2.1 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 72 1.7999999999999998 No Hit GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 52 1.3 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 51 1.275 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 50 1.25 No Hit GGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAG 45 1.125 No Hit GTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAG 42 1.05 No Hit CTCCAGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTG 40 1.0 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 40 1.0 No Hit GCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATG 40 1.0 No Hit GGCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCC 39 0.975 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 36 0.8999999999999999 No Hit GTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCAGGG 31 0.775 No Hit GCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTT 31 0.775 No Hit GCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTT 31 0.775 No Hit GTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCA 30 0.75 No Hit GTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAAGACTCCT 30 0.75 No Hit AGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 30 0.75 No Hit GGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTC 29 0.7250000000000001 No Hit GGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGG 26 0.65 No Hit CTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTT 26 0.65 No Hit GGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCT 25 0.625 No Hit GTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCA 25 0.625 No Hit ATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGA 23 0.575 No Hit GTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAG 22 0.5499999999999999 No Hit GGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCT 22 0.5499999999999999 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 22 0.5499999999999999 No Hit CCAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGC 21 0.525 No Hit CCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGC 21 0.525 No Hit GCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAA 21 0.525 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 20 0.5 No Hit ATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCAACGTAC 19 0.475 No Hit CTCCAATTAAGCTCTTTTTTCAGAATTTTAACAATCTCACGCCTTGGCAC 19 0.475 No Hit GACAGGTTTCTGGGATGGCGGATAGCATCAGATATTCTCTCTGCATGATT 19 0.475 No Hit GTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCTTCACGCA 18 0.44999999999999996 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 18 0.44999999999999996 No Hit CCCTGATGTGTTGAGTCAACAACTCCTCCTTGAGCCACCTCATCTAACAG 18 0.44999999999999996 No Hit ATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCT 18 0.44999999999999996 No Hit CACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCT 17 0.42500000000000004 No Hit GCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGCGGAT 17 0.42500000000000004 No Hit CTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGAT 17 0.42500000000000004 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 17 0.42500000000000004 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 17 0.42500000000000004 No Hit GTGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCT 17 0.42500000000000004 No Hit ATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAG 16 0.4 No Hit CCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAA 16 0.4 No Hit GTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCT 15 0.375 No Hit CGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGC 15 0.375 No Hit CCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAAC 14 0.35000000000000003 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 14 0.35000000000000003 No Hit GCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAA 14 0.35000000000000003 No Hit CCCACATCCAACACCATGCAGAGTGTTCATGGACAGTCTTCTTTTTCTCC 14 0.35000000000000003 No Hit TTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAAGACTCCT 14 0.35000000000000003 No Hit GCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTT 14 0.35000000000000003 No Hit CATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCA 13 0.325 No Hit CGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCT 13 0.325 No Hit ATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAA 13 0.325 No Hit CTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCCT 13 0.325 No Hit GAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTAC 13 0.325 No Hit CCAGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCA 12 0.3 No Hit CTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGA 12 0.3 No Hit GCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCC 12 0.3 No Hit GTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAGATATTCTCTCTGCA 12 0.3 No Hit CGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTT 12 0.3 No Hit ACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCA 12 0.3 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 12 0.3 No Hit TTTTTTTTTTCCACTTGGAATTCCTTCTCGGTTCAATTTTCAATAATGCT 12 0.3 No Hit ATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCAT 12 0.3 No Hit CTTTCCCAGAAAGTAATCCCAAACCAAATGCTACTGCACTAGCTGATGCT 12 0.3 No Hit GCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCAACAACTCCTCCTT 12 0.3 No Hit GCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAGA 11 0.27499999999999997 No Hit CTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCG 11 0.27499999999999997 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 11 0.27499999999999997 No Hit CAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTTC 11 0.27499999999999997 No Hit GTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGC 11 0.27499999999999997 No Hit GCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAA 11 0.27499999999999997 No Hit GGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTA 11 0.27499999999999997 No Hit CCTCAGTTACTTGAAGAGCAAATTTTTGCACCTTAACCCTTTCCTGGAGT 10 0.25 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTT 10 0.25 No Hit CAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCAC 10 0.25 No Hit GGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTA 10 0.25 No Hit GGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTT 10 0.25 No Hit CAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCA 10 0.25 No Hit CCTTGTTCTTGACGCAGCCCAATCTTTATTAATGAGGAGAACTAAACATG 10 0.25 No Hit CCAGAAAGTAATCCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGG 10 0.25 No Hit CTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTT 10 0.25 No Hit GATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTT 10 0.25 No Hit ACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGA 10 0.25 No Hit CCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCC 10 0.25 No Hit GCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCAGGGGCACAA 10 0.25 No Hit AGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCAGG 10 0.25 No Hit GGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCATGCA 10 0.25 No Hit ATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCT 9 0.22499999999999998 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACA 9 0.22499999999999998 No Hit CAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAG 9 0.22499999999999998 No Hit CATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTT 9 0.22499999999999998 No Hit GTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTC 9 0.22499999999999998 No Hit GCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCA 9 0.22499999999999998 No Hit GTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAG 9 0.22499999999999998 No Hit GTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCG 9 0.22499999999999998 No Hit TTTTTTTTTTTCCACTTGGAATTCCTTCTCGGTTCAATTTTCAATAATGC 9 0.22499999999999998 No Hit TTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCAT 8 0.2 No Hit CAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAAT 8 0.2 No Hit GCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCA 8 0.2 No Hit GCTAGCGAATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATG 8 0.2 No Hit CATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTA 8 0.2 No Hit CGCCTGAGTGGTTTTGCAGAATGTGATCAACTATGCTGGTGATGTCCCTT 8 0.2 No Hit CCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTT 8 0.2 No Hit TTTTTTTTTTCCAGAATAAAAGTCTTGCTTGATATTATCATTAAAAGCTG 8 0.2 No Hit TTCTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTG 8 0.2 No Hit GGTCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTT 8 0.2 No Hit CTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCT 8 0.2 No Hit GAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTTCAACTCCAG 8 0.2 No Hit GCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTA 7 0.17500000000000002 No Hit CTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 7 0.17500000000000002 No Hit TTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAAGACTCCTGG 7 0.17500000000000002 No Hit GAGTCGAGTTCTTTAGCCTTTTAATTGTTTTTCCACAGGCATGTATCTGG 7 0.17500000000000002 No Hit GCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCA 7 0.17500000000000002 No Hit ACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCAGGGGA 7 0.17500000000000002 No Hit GTCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCAT 7 0.17500000000000002 No Hit GTATTTTCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTT 7 0.17500000000000002 No Hit CCAACACCATGCAGAGTGTTCATGGACAGTCTTCTTTTTCTCCAATTAAG 7 0.17500000000000002 No Hit GCTAAATAAGAGTTCAACTCCAGTACAAAGAAGTTTTCATATTCCAGTCA 7 0.17500000000000002 No Hit CTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCATCTTGTTCCATT 7 0.17500000000000002 No Hit AGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGA 7 0.17500000000000002 No Hit AGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTTCAACTCCAGT 7 0.17500000000000002 No Hit GCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAAT 7 0.17500000000000002 No Hit TTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGAAGACTCCTG 7 0.17500000000000002 No Hit GTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTT 7 0.17500000000000002 No Hit TGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 7 0.17500000000000002 No Hit GTGACCATGATTGGAGAGCAAACAATCAAATTTCTTGACCATCTACTAAT 7 0.17500000000000002 No Hit TTTTTTTTCCACTTGGAATTCCTTCTCGGTTCAATTTTCAATAATGCTTA 7 0.17500000000000002 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 7 0.17500000000000002 No Hit GCTGCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGCG 7 0.17500000000000002 No Hit TGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGT 7 0.17500000000000002 No Hit CTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAA 7 0.17500000000000002 No Hit GGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTG 7 0.17500000000000002 No Hit GCAGGATACTTGCTATTTTTGCCAACTTCTTATCCAGATACAGGCAGTTC 7 0.17500000000000002 No Hit AGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCAGGGGATGGG 7 0.17500000000000002 No Hit CATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGC 6 0.15 No Hit GTTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATG 6 0.15 No Hit GAGACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTT 6 0.15 No Hit AGATTGTAAAGCTAAATAAGAGTTCAACTCCAGTACAAAGAAGTTTTCAT 6 0.15 No Hit GCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCA 6 0.15 No Hit GGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCAAC 6 0.15 No Hit GTTACGGCGCAAATGCATAACAAATTGTACTCATGATTAGTCCAGAAATA 6 0.15 No Hit GTCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAG 6 0.15 No Hit AAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAA 6 0.15 No Hit GTCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTG 6 0.15 No Hit CCCAAACCAAATGCTACTGCACTAGCTGATGCTCGAGGAACCTGAGTTGC 6 0.15 No Hit CCATGGCCATAGTATGGCTCGGGCACAGACCACCTTGCACTACGATATAT 6 0.15 No Hit GTCTTGGCCTTGAGATTGACAACAGATGGATCCGCTCCTAGGAGGTCACA 6 0.15 No Hit TTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAACACGGCGTCTC 6 0.15 No Hit TTTTTTTTTCTACGCCTTCTTGGGTTCTCATATCTTCAAGTCATCTAGTG 6 0.15 No Hit TGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 6 0.15 No Hit CGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAG 6 0.15 No Hit CCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTT 6 0.15 No Hit CGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAAGTCACTGA 6 0.15 No Hit GGACAGTCTTCTTTTTCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAA 6 0.15 No Hit GCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGCAGAA 6 0.15 No Hit GCCTGAGTGGTTTTGCAGAATGTGATCAACTATGCTGGTGATGTCCCTTT 6 0.15 No Hit GAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCC 6 0.15 No Hit AAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGC 6 0.15 No Hit GGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGC 6 0.15 No Hit TTTTTTTTTTTCCATACATCTCTAGAAAACACCATTCAGGGATATCAGTG 6 0.15 No Hit AGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGGC 6 0.15 No Hit GTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCA 6 0.15 No Hit GTTCAACTCCAGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAAT 6 0.15 No Hit ACGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAG 6 0.15 No Hit GACATCATGAGTTTTTCCCTTCCCCTTCTTTTTAGGCTTGTTCTCTTTCA 6 0.15 No Hit CTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATGG 6 0.15 No Hit GCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGAT 6 0.15 No Hit AATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCA 6 0.15 No Hit GCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCATC 6 0.15 No Hit ACCTTGTTCTTGACGCAGCCCAATCTTTATTAATGAGGAGAACTAAACAT 6 0.15 No Hit CCGACATCATCAAGACTCAAAGGGGGAAAACTGGTAATTTAAGAGCGGAA 6 0.15 No Hit GTCCGATTTGCGCAGATCATGGGATCTGTTCCATGATCAAAAAGTCAAGT 6 0.15 No Hit GGCAAGGTAACTCTATTACTCAAGAACGTACACGAATACTGAGTGAAAAA 6 0.15 No Hit CCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTG 6 0.15 No Hit TGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCT 6 0.15 No Hit TTTTTTTTTCCAGAATAAAAGTCTTGCTTGATATTATCATTAAAAGCTGT 6 0.15 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 6 0.15 No Hit CCATCACGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCT 6 0.15 No Hit GCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTT 6 0.15 No Hit GTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAA 6 0.15 No Hit CATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAAC 6 0.15 No Hit AACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAGAAGT 5 0.125 No Hit GCTCGGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCA 5 0.125 No Hit TTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAAT 5 0.125 No Hit CTTCCTGCTGCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGG 5 0.125 No Hit GCCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 5 0.125 No Hit CTTGAGATTGACAACAGATGGATCCGCTCCTAGGAGGTCACACAAGTTAG 5 0.125 No Hit CGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCT 5 0.125 No Hit GTACTTTGTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAGATATTCT 5 0.125 No Hit AATGCCATCACGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCC 5 0.125 No Hit TCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTT 5 0.125 No Hit GGCAGTCTTCTTTTTCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAAT 5 0.125 No Hit AAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCAGGGGATG 5 0.125 No Hit CGCTCCTTCTGGAATTGCAAAGGGGTCCTCAGTTACTTGAAGAGCAAATT 5 0.125 No Hit CCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCCCTTC 5 0.125 No Hit ACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAAC 5 0.125 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 5 0.125 No Hit GCACTACGATATATCCCACATCCAACACCATGCAGAGTGTTCATGGACAG 5 0.125 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTATACGAAACATCAAACAGCTTTT 5 0.125 No Hit GTTCATGGACAGTCTTCTTTTTCTCCAATTAAGCTCTTTTTTCAGAATTT 5 0.125 No Hit GGAGGTCACACAAGTTAGATCGGATCGTCTCCTTGAATGGGCTAATTTTT 5 0.125 No Hit CAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTGGCATGG 5 0.125 No Hit CTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTT 5 0.125 No Hit CCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 5 0.125 No Hit CCTCCACCGACACAGTCAACAGCCCACTGGAGGAGCACAAGTCCGACGCG 5 0.125 No Hit GGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCATGCAG 5 0.125 No Hit GTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTCCACAA 5 0.125 No Hit TTTTTTTTTCGAAGAAAAAAAATTATAAATCAAATCTCAATTTCGGTACA 5 0.125 No Hit CTCTAAAGCTAATATGCGTGCATGAGCACACATATTTCTCCGATAATCAA 5 0.125 No Hit CGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTT 5 0.125 No Hit CTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCAGGGGCACAAG 5 0.125 No Hit GCCTTTTTCAATTTTGTACCCAAATCTACACTCCCAGAGATGATCGCGTC 5 0.125 No Hit GCGGTAGTTCAAGAGTGTCCTACAGGGGGCTTTAGCACATCTCCAAAACA 5 0.125 No Hit AACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGC 5 0.125 No Hit CAGTAATACTACTATGTGCATTAATTATATTGTATACTATTATACACAGT 5 0.125 No Hit GCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAAT 5 0.125 No Hit TTTTTTTTTTTTCTACGCCTTCTTGGGTTCTCATATCTTCAAGTCATCTA 5 0.125 No Hit GATTTCGTTTATACGAAACATCAAACAGCTTTTTTTTCATGCCGCCCTTC 5 0.125 No Hit ACCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAAT 5 0.125 No Hit TTTTTTTCGAAGAAAAAAAATTATAAATCAAATCTCAATTTCGGTACAGG 5 0.125 No Hit GAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTG 5 0.125 No Hit AAATGATGTGTGGGGCCTTTACGCCTTACTTATTCCATCAGTTTGCCAAT 5 0.125 No Hit TGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTTCAACTCCA 5 0.125 No Hit ATCCGTCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTTTTTTTG 5 0.125 No Hit TTTTTTTTTTTCAAGGACGTACGGCGAGACATAGTATTATCATCCCACGT 5 0.125 No Hit AGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTT 5 0.125 No Hit GCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTT 5 0.125 No Hit ATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCTTG 20 0.005129473 29.872398 140-144 >>END_MODULE ERR5262798 read2 length is 79-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262798_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 79-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.994 37.0 37.0 37.0 37.0 37.0 2 35.952 37.0 37.0 37.0 37.0 37.0 3 36.007 37.0 37.0 37.0 37.0 37.0 4 36.0905 37.0 37.0 37.0 37.0 37.0 5 36.1905 37.0 37.0 37.0 37.0 37.0 6 36.2555 37.0 37.0 37.0 37.0 37.0 7 36.1925 37.0 37.0 37.0 37.0 37.0 8 36.2745 37.0 37.0 37.0 37.0 37.0 9 36.299 37.0 37.0 37.0 37.0 37.0 10-14 36.191500000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.24810000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.1496 37.0 37.0 37.0 37.0 37.0 25-29 36.112399999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.0769 37.0 37.0 37.0 37.0 37.0 35-39 35.9905 37.0 37.0 37.0 37.0 37.0 40-44 36.042899999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.0081 37.0 37.0 37.0 37.0 37.0 50-54 35.9142 37.0 37.0 37.0 37.0 37.0 55-59 35.8836 37.0 37.0 37.0 37.0 37.0 60-64 35.8846 37.0 37.0 37.0 37.0 37.0 65-69 35.9025 37.0 37.0 37.0 37.0 37.0 70-74 35.8167 37.0 37.0 37.0 37.0 37.0 75-79 35.7193 37.0 37.0 37.0 37.0 37.0 80-84 35.65186296574144 37.0 37.0 37.0 37.0 37.0 85-89 35.676789678140615 37.0 37.0 37.0 37.0 37.0 90-94 35.688532799198796 37.0 37.0 37.0 37.0 37.0 95-99 35.64632969220637 37.0 37.0 37.0 37.0 37.0 100-104 35.49909517365977 37.0 37.0 37.0 37.0 37.0 105-109 35.46249409549766 37.0 37.0 37.0 32.2 37.0 110-114 35.49949346778228 37.0 37.0 37.0 37.0 37.0 115-119 35.491432988972925 37.0 37.0 37.0 37.0 37.0 120-124 35.40933889584848 37.0 37.0 37.0 34.6 37.0 125-129 35.367394585087006 37.0 37.0 37.0 34.6 37.0 130-134 35.273047816631035 37.0 37.0 37.0 32.2 37.0 135-139 35.243241737808475 37.0 37.0 37.0 29.8 37.0 140-144 35.20976443113993 37.0 37.0 37.0 29.8 37.0 145-149 35.08689264198417 37.0 37.0 37.0 27.4 37.0 150 35.12050163576881 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 1.0 14 0.0 15 0.0 16 1.0 17 1.0 18 1.0 19 0.0 20 1.0 21 2.0 22 2.0 23 4.0 24 7.0 25 9.0 26 15.0 27 11.0 28 17.0 29 20.0 30 38.0 31 44.0 32 68.0 33 128.0 34 266.0 35 676.0 36 2485.0 37 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.075 22.875 7.9 19.15 2 31.674999999999997 21.925 27.825 18.575 3 20.349999999999998 27.650000000000002 33.900000000000006 18.099999999999998 4 25.775 30.975 22.85 20.4 5 25.3 33.5 21.45 19.75 6 25.5 35.975 19.375 19.15 7 21.325 19.975 37.55 21.15 8 22.6 22.2 26.8 28.4 9 20.075000000000003 25.724999999999998 29.025000000000002 25.174999999999997 10-14 23.89 26.845000000000002 27.134999999999998 22.13 15-19 24.3 25.785000000000004 27.73 22.185 20-24 24.325 26.840000000000003 26.915 21.92 25-29 24.38 26.185000000000002 28.175 21.26 30-34 24.625 27.555000000000003 26.51 21.310000000000002 35-39 24.6 25.8 27.36 22.24 40-44 24.645 26.86 26.775 21.72 45-49 23.32 26.69 28.299999999999997 21.69 50-54 23.535 27.1 26.895000000000003 22.470000000000002 55-59 23.265 26.36 27.26 23.115 60-64 23.119999999999997 28.075 27.185 21.62 65-69 23.195 28.055000000000003 28.53 20.22 70-74 24.27 27.3 27.32 21.11 75-79 23.32 27.565 28.689999999999998 20.424999999999997 80-84 22.88072018004501 27.941985496374095 29.312328082020507 19.86496624156039 85-89 24.038028521391045 26.159619714786093 28.486364773580185 21.31598699024268 90-94 24.096144216324486 27.30595893840761 27.38607911867802 21.211817726589885 95-99 24.25244177310293 27.07738542449286 27.638367142499376 21.031805659904833 100-104 24.538430664258478 26.71081677704194 27.633955448524983 21.11679711017459 105-109 24.421645544156107 27.740897203781934 27.891772279219474 19.945684972842486 110-114 23.759580475998387 27.359822509076242 27.717829770068576 21.1627672448568 115-119 22.48580697485807 28.17315490673155 27.691605839416056 21.649432278994325 120-124 22.778683513348277 26.885062156103523 28.62747095985327 21.708783370694924 125-129 22.405406789206904 27.556192719266807 28.62628641646613 21.41211407506016 130-134 23.950795947901593 27.134587554269174 28.63345048583833 20.281166011990905 135-139 22.88980529310435 26.402881453254682 29.007673435297804 21.699639818343165 140-144 22.507408975444537 26.973962743437763 29.064352243861137 21.454276037256562 145-149 22.458565027263404 27.938238946175026 28.56448739405064 21.038708632510932 150 25.763358778625957 26.39040348964013 29.634678298800438 18.21155943293348 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.0 25 0.5 26 0.0 27 0.5 28 0.5 29 0.5 30 3.0 31 6.5 32 5.0 33 17.5 34 32.5 35 40.0 36 75.5 37 105.5 38 130.5 39 180.0 40 221.0 41 289.5 42 315.5 43 287.5 44 334.0 45 315.5 46 195.0 47 158.5 48 153.5 49 137.5 50 137.5 51 109.5 52 72.0 53 64.0 54 70.0 55 63.5 56 58.0 57 51.5 58 46.0 59 39.5 60 31.0 61 32.5 62 29.5 63 18.5 64 15.0 65 12.0 66 9.5 67 14.5 68 26.0 69 22.0 70 12.5 71 11.5 72 10.5 73 9.5 74 8.5 75 7.5 76 4.0 77 2.5 78 1.5 79 0.0 80 0.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 78-79 1.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 5.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.0 96-97 0.0 98-99 1.0 100-101 8.0 102-103 2.0 104-105 2.0 106-107 6.0 108-109 2.0 110-111 6.0 112-113 3.0 114-115 15.0 116-117 8.0 118-119 7.0 120-121 9.0 122-123 3.0 124-125 9.0 126-127 14.0 128-129 12.0 130-131 13.0 132-133 24.0 134-135 12.0 136-137 9.0 138-139 19.0 140-141 28.0 142-143 37.0 144-145 29.0 146-147 20.0 148-149 27.0 150-151 3668.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.225 #Duplication Level Percentage of deduplicated Percentage of total 1 48.60864416814683 20.525 2 21.136767317939608 17.849999999999998 3 11.486086441681469 14.549999999999999 4 6.927175843694494 11.700000000000001 5 4.381290704558911 9.25 6 2.782711663706335 7.049999999999999 7 1.3025458851391356 3.85 8 0.7104795737122558 2.4 9 0.8288928359976317 3.15 >10 1.8354055654233272 9.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 22 0.5499999999999999 No Hit GTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGA 18 0.44999999999999996 No Hit GTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTG 17 0.42500000000000004 No Hit GTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTT 16 0.4 No Hit GTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGA 16 0.4 No Hit GTTGGGCAAAACATCTCATACAGATCCAAGATTACCATCAAGCAGTGATA 16 0.4 No Hit GGCACGACCATTCAAAAGATTGAGGGTTCATACTCAAGCAGGAGGAAATA 14 0.35000000000000003 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 14 0.35000000000000003 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 13 0.325 No Hit GTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGA 13 0.325 No Hit GGAACTTCTTGATGCCTATTACACCAATATGCGCTTTAGAAGCCTCAACG 13 0.325 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCGGCTCTGACTATGACTG 12 0.3 No Hit GTGCATCTCTCGCTATTCCATAGTAGTTGATTATGCCTTAAGAACACTTG 12 0.3 No Hit GGAAAATTCAGCGGTTCCTTAGCCAGCCTTTTCATGTTGCTGAAGTGTTC 12 0.3 No Hit AGAGGCTGCACTAGGCATCGATTTGCGTCATCGAGGTTTGCAGGTCCGGT 12 0.3 No Hit CAAACCCTTTGAGACCCAGTACAATTGAAAAAGATCCACTGGCTCATTTG 11 0.27499999999999997 No Hit GTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTTG 11 0.27499999999999997 No Hit GCTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAA 11 0.27499999999999997 No Hit GCAATATCGATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATT 11 0.27499999999999997 No Hit GTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCC 11 0.27499999999999997 No Hit GCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAA 11 0.27499999999999997 No Hit GCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGC 11 0.27499999999999997 No Hit GCGCACCCTATCAGCAATGCTTGGTACTGGACGGAAGTTTGAGAGTACCA 10 0.25 No Hit GTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCCACAACT 10 0.25 No Hit GACTGATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTG 10 0.25 No Hit GTTGATTATGCCTTAAGAACACTTGTCAGACCTCGAGAAGATTCAAAATT 10 0.25 No Hit ATGGAGGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAAATTTGAC 10 0.25 No Hit GCACCAACAAGTTCGTCGGCCCACTTCCTGAAGTAGTAGAAAACTTGACC 10 0.25 No Hit GGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAG 10 0.25 No Hit CTGGGATCACCTTACATTGCATAGGTCTCTCAATTGTTTCTTTGGCGTGA 10 0.25 No Hit AATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCAT 10 0.25 No Hit ATCGAGGTTTGCAGGTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGA 9 0.22499999999999998 No Hit CTTACATTGCATAGGTCTCTCAATTGTTTCTTTGGCGTGAGTCCGTTCTC 9 0.22499999999999998 No Hit GCCAGCCTTTTCATGTTGCTGAAGTGTTCACAGGTGCACCTGGAAAGTAT 9 0.22499999999999998 No Hit AAATAGTCATCAGCGCTTCTATATTCATGTCCAGGGGCTAAACAAACTAC 9 0.22499999999999998 No Hit CGGGGCTTTCGTTGGCATGAACCACTATAATGAACGTAAAGAAACTCTTT 9 0.22499999999999998 No Hit GGAGAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGGTCCACCG 9 0.22499999999999998 No Hit GGAGCACTACAACACTGCTCGTGGTGTCCAGAAGGTTCTCCAGAATTACA 9 0.22499999999999998 No Hit GGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGC 9 0.22499999999999998 No Hit GCGCAGAATAAATGAAGTTCTAGAAGTGCATGTTGACCCCCATATGGATC 9 0.22499999999999998 No Hit AGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCA 9 0.22499999999999998 No Hit AAACAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTGTGTC 9 0.22499999999999998 No Hit GCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAAACCC 9 0.22499999999999998 No Hit ATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAA 9 0.22499999999999998 No Hit ACTCTCTGGTTCTTCTCAACTTCGGATGGGATCAACAAGAGAAAATGGGC 9 0.22499999999999998 No Hit GTTCATACTCAAGCAGGAGGAAATAGTCATCAGCGCTTCTATATTCATGT 8 0.2 No Hit GGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTG 8 0.2 No Hit TGGAAAAAAAAATGGAAAAAAAAATGAAAAGAGAGGGGCACGACCATTCA 8 0.2 No Hit GGACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGCCGTGGC 8 0.2 No Hit GCTGACTTCATTCTGACTTACACTGATGAGGACGGGGATGTTGTCATGCT 8 0.2 No Hit GGGCCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGAT 8 0.2 No Hit GATGATGAACATTCTCTGGATGGCCAAATAAAGGGATACTCAATATCAGA 8 0.2 No Hit CATCAGTGTTGATGCCTCTCTCACACACAAATGGAAAAAAAAATGGAAAA 8 0.2 No Hit CTTGCATGATGCAGCTATCAAGCAGGAGCTGAATCCTCTGAGGATTAATG 8 0.2 No Hit ATCGATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATA 8 0.2 No Hit GTCATTAGCAACAGATTTGACACAGAATACTTGCTCGGATGTCGTTCTCG 8 0.2 No Hit AGTCATCAGCGCTTCTATATTCATGTCCAGGGGCTAAACAAACTACTACC 8 0.2 No Hit ATTATAGCGAGGATGGGGTGGATCTTGTTGTTAAAGATCCTAATGGAGGT 7 0.17500000000000002 No Hit GGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTG 7 0.17500000000000002 No Hit GCTAAACAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTGT 7 0.17500000000000002 No Hit GCTGCACTAGGCATCGATTTGCGTCATCGAGGTTTGCAGGTCCGGTGATC 7 0.17500000000000002 No Hit CAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCA 7 0.17500000000000002 No Hit GGTCTGGATCATCGCAAATGGATGGCACAATGGAGACCACCCTGCTTCAC 7 0.17500000000000002 No Hit AGTATGTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGAT 7 0.17500000000000002 No Hit GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 7 0.17500000000000002 No Hit AGCACCAACAAGTTCGTCGGCCCACTTCCTGAAGTAGTAGAAAACTTGAC 7 0.17500000000000002 No Hit AACAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTGTGTCC 7 0.17500000000000002 No Hit GTCATATGTCATGTGTACTTGAGCCTGGGGCTGTGCTGTTCAATAAATAT 7 0.17500000000000002 No Hit GGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATC 7 0.17500000000000002 No Hit GAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCT 7 0.17500000000000002 No Hit CGACCATTCAAAAGATTGAGGGTTCATACTCAAGCAGGAGGAAATAGTCA 7 0.17500000000000002 No Hit GCCGGCGGGCGGCGAGAAGCTCTGTGCGGCAAGTTGTTGCTCTTCTGTTG 7 0.17500000000000002 No Hit GGCTGCACTAGGCATCGATTTGCGTCATCGAGGTTTGCAGGTCCGGTGAT 7 0.17500000000000002 No Hit GGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAAC 7 0.17500000000000002 No Hit ATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAA 7 0.17500000000000002 No Hit GTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAAATTTGACTTCATT 7 0.17500000000000002 No Hit GGTGCACACTCAAAGCTTGTTCATACATGGATCTATATTTCTTTATGAAG 7 0.17500000000000002 No Hit AGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAG 7 0.17500000000000002 No Hit GTGACATTGCGAATTTCTCTGATAATCCAGTGAATTTTCACCTCCTGCTC 7 0.17500000000000002 No Hit CATAGACTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAACAACATG 6 0.15 No Hit GGTGTATGGAATGTCATATGTCATGTGTACTTGAGCCTGGGGCTGTGCTG 6 0.15 No Hit CAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCCACA 6 0.15 No Hit CAACAGGCTGTCTAATCTCTGCAGACGCTACTGTGAGCTATCCCAATATT 6 0.15 No Hit CCTGGAAGTTTTGACCGGGTTGTAGAGGCTGCACTAGGCATCGATTTGCG 6 0.15 No Hit TGATACTCCAGAGTATCTTTCCTATGTTGAGCAATTTGGTATATCACATC 6 0.15 No Hit GGTGCATATCTCTGAAGTTATCCATTACTAATGTAGGGTCCTCCATTATG 6 0.15 No Hit GCCGCCGTTCGACGGCCTCGCGCCGGGACCCAACTCCGAATGGGATGTTG 6 0.15 No Hit GGGAGTACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGT 6 0.15 No Hit GTCCGGTGATCCACTGCTCCAAGATGGAGCAGGAGAATTGGCTTGCGCCC 6 0.15 No Hit GTATGGAATGTCATATGTCATGTGTACTTGAGCCTGGGGCTGTGCTGTTC 6 0.15 No Hit GGGACATTGAATGTGCAAGTTGGCCGATAATGAAAGGTGTATTCTCCTTT 6 0.15 No Hit GATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACT 6 0.15 No Hit GATTGTTCCAAATCGGAGACGCATGTTTTAAATTTCTCTGCAGTTAGTAA 6 0.15 No Hit CGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGG 6 0.15 No Hit GGAGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTAT 6 0.15 No Hit ATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGA 6 0.15 No Hit GGGTCCTCCATTATGGTATATTGAAATTTCGGTTCATTTTTTTAATCATC 6 0.15 No Hit CCCATCTCTACAAGAATAAAGCTTTATTAGCAGGTGGTGGTCTGGATCAT 6 0.15 No Hit AGAGAGGGGCACGACCATTCAAAAGATTGAGGGTTCATACTCAAGCAGGA 6 0.15 No Hit ATTGAGGGTTCATACTCAAGCAGGAGGAAATAGTCATCAGCGCTTCTATA 6 0.15 No Hit TGACCCCCATATGGATCCTGATTATGAATCTACTGAAGAAATGGATTATC 6 0.15 No Hit TCAACGCGCAGGAAGTGCAAGAAGGCTCATAAAAGCAGCTCTCCAAGAAG 6 0.15 No Hit TGTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCA 6 0.15 No Hit TGGTCTGGATCATCGCAAATGGATGGCACAATGGAGACCACCCTGCTTCA 6 0.15 No Hit ATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCGGCTCTGACTATGAC 6 0.15 No Hit GCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACAT 6 0.15 No Hit GTCATCAGCGCTTCTATATTCATGTCCAGGGGCTAAACAAACTACTACCT 6 0.15 No Hit GGTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGT 6 0.15 No Hit GCAGGTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTA 6 0.15 No Hit CAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCC 6 0.15 No Hit GGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTC 6 0.15 No Hit CGATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGG 6 0.15 No Hit AATGGGGAGCCTATCTCCCATGGTTCAAGTCCTGTTCTCAACAAACAGCA 6 0.15 No Hit TGGCTCATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGT 6 0.15 No Hit GACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTC 6 0.15 No Hit CTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTCAATTGTTTC 6 0.15 No Hit GGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTC 6 0.15 No Hit GCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAGACTTTGAC 6 0.15 No Hit AAAGATCCTAATGGAGGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGA 6 0.15 No Hit GTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAGA 6 0.15 No Hit CTTTCATGCCATCGTCCCCCATCTTTTCTTTTGGCGTGTATCCTCGATCA 6 0.15 No Hit CATCAGCGCTTCTATATTCATGTCCAGGGGCTAAACAAACTACTACCTAT 6 0.15 No Hit ATTGGATATGGCTCGGCTCTGACTATGACTGATACATCAAATAAGATAAA 6 0.15 No Hit CGGCAGCTAATTGGATTGATGAAGGTATGGTAAAGAGGATAAGAGGTGTA 6 0.15 No Hit AAATGAAGTTCTAGAAGTGCATGTTGACCCCCATATGGATCCTGATTATG 6 0.15 No Hit CAATATTGATGAAATGAGTAAAGAATCTGAGAAGCCTGAGCTAATGTCTC 6 0.15 No Hit CTTCCGTCCTCGCAAATCTCTTCCTCTTCTGTTAGGGTTAGGTCCCGCGG 5 0.125 No Hit GGAAAAGCTACAGCTTCTCAACTCTTCTCAAGAAAGAGCGCGCAGAATAA 5 0.125 No Hit GGTGGAGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGA 5 0.125 No Hit GCAATTTCAGGTGAAACTGATGCATCAAGCAGGGTATGAGCTGGGGAACC 5 0.125 No Hit GGAGGCGCTCACCAACGAGATGGACGAGGAGTCACAGATCGTCCGGGAGG 5 0.125 No Hit CTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTT 5 0.125 No Hit AGCGATGATGAGGATGCATATGCCTATAGTAATCAAATAACTGAGGAGCA 5 0.125 No Hit CAATTGTTTCTTTGGCGTGAGTCCATTCTCTCCCCCATCCCCTGCACACT 5 0.125 No Hit GCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTC 5 0.125 No Hit CTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAAG 5 0.125 No Hit GAATACTTGCTCGGATGTCGTTCTCGAGTACTTTCGTCCACTTGTAGAAT 5 0.125 No Hit AGGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAAATTTGACTTCA 5 0.125 No Hit GTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTT 5 0.125 No Hit GCGTGCTGCTTTAGGAATGGATGCGGATAGGTACGCTGCATTTAGAGAAA 5 0.125 No Hit GAAATAGTCATCAGCGCTTCTATATTCATGTCCAGGGGCTAAACAAACTA 5 0.125 No Hit GGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAA 5 0.125 No Hit TGTTTCTGGTGCGTCCTTTGGCCAGACATCATCAAGTACACCTTACGCCA 5 0.125 No Hit CCTGCACACTATTTTTTCCTATGACTGGAATATGAAAACTTCTTTGTACT 5 0.125 No Hit TGGCGTCGATGGTCTTCTGCGAGGCGCCGCTGGACGCCTACGGCACGTCG 5 0.125 No Hit GTTGAGGAGTTGGGCAAAACATCTCATACAGATCCAAGATTACCATCAAG 5 0.125 No Hit GTCGGAGGTTCACACGGAGGTTTCTTGACAACACATTTGATCGGCCAGGC 5 0.125 No Hit GTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAAT 5 0.125 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 5 0.125 No Hit AGCAGGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGAACAGCCAT 5 0.125 No Hit GGAAAAAAAAATGGAAAAAAAAATGAAAAGAGAGGGGCACGACCATTCAA 5 0.125 No Hit AAAGAAACTCTTTTCGTGCCAAGGCGTGAGATTGTTAAAATTCTGAAAAA 5 0.125 No Hit GCTGGTTAGTGAAGGCGAGGAAGAGTTGTTCAATTTTGCTAACAGAGTGA 5 0.125 No Hit AATACTTTTTTCTTAGCATGTGATAGAATAGGTGTTCGTTTGGGGCACAT 5 0.125 No Hit GGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAG 5 0.125 No Hit GGAATGTCATATGTCATGTGTACTTGAGCCTGGGGCTGTGCTGTTCAATA 5 0.125 No Hit GGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTT 5 0.125 No Hit AGTACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGC 5 0.125 No Hit GAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTT 5 0.125 No Hit TCTCAATTGTTTCTTTGGCGTGAGTCCGTTCTCTCCCCCATCCCCTGCAC 5 0.125 No Hit AGAAGACGACAAATTTTGCACTTGAGATATTAAATCTAAACAAAAAAGAG 5 0.125 No Hit CTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCAATAAAT 5 0.125 No Hit GGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTCAA 5 0.125 No Hit CTTTCGTCCACTTGTAGAATCAAGAAGGCTGGGATTGGAGGCCCCCTTAT 5 0.125 No Hit TGTAACAGTTCATTAATCTGGCTCAGTAATCTTTTTTGAGTGTAAATCTG 5 0.125 No Hit TGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAG 5 0.125 No Hit TGATGAAGCTTTAAAACCCACACCAAACCCTTTGAGACCCAGTACAATTG 5 0.125 No Hit GTGGTCCTCTGTGTGCTGCCCTTCTTGCGCCCGGCTGCGGGTATCCGCTT 5 0.125 No Hit AATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACC 5 0.125 No Hit CTCAATTGTTTCTTTGGCGTGAGTCCGTTCTCTCCCCCATCCCCTGCACA 5 0.125 No Hit GCAAATCGCGTTTCGCCATGGCTCCGAGCACGTCTCTTCCCTTCCTCCTC 5 0.125 No Hit GGTGCCCGGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTT 5 0.125 No Hit AACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTAC 5 0.125 No Hit GCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGTGGTCA 5 0.125 No Hit TGCAAGTGCTTTTAAGTTCGCCCTGGATGCTGACTTCATTCTGACTTACA 5 0.125 No Hit CAGTTATTATGTATACAAGTGGGAGTACAGGTCTGCCCAAGGGTGTAATG 5 0.125 No Hit CCTTACATTGCATAGGTCTCTCAATTGTTTCTTTGGCGTGAGTCCGTTCT 5 0.125 No Hit AATACCGCCAAGGTGCCATTGATACTCCAGAGTATCTTTCCTATGTTGAG 5 0.125 No Hit GGTGGCCACAACTGCTGCCGTCAGGACAATCATTCCTAAACTTGGCACGG 5 0.125 No Hit CACGACCATTCAAAAGATTGAGGGTTCATACTCAAGCAGGAGGAAATAGT 5 0.125 No Hit CAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGAGCA 5 0.125 No Hit AAATGGAAAAAAAAATGGAAAAAAAAATGAAAAGAGAGGGGCACGACCAT 5 0.125 No Hit CAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCA 5 0.125 No Hit CATTGGCCCAAACTGTACTTTTGATATGTGCAATTGGTAAAGATGCTGAC 5 0.125 No Hit GGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAAT 5 0.125 No Hit AGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATCC 5 0.125 No Hit ACTCAAGTTCAGCCTTTAACCGAGAGTTATTGGGAAGAGAAGTGGAGAAA 5 0.125 No Hit TTCACTCTGAATTTATCTGCTCTTCGGTCCAAAATTGCAAGTGCTTTTAA 5 0.125 No Hit CTCATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTT 5 0.125 No Hit GGCTAAACAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTG 5 0.125 No Hit AACACATTTCACTCTGAATTTATCTGCTCTTCGGTCCAAAATTGCAAGTG 5 0.125 No Hit GGTTCATACTCAAGCAGGAGGAAATAGTCATCAGCGCTTCTATATTCATG 5 0.125 No Hit GCGGCGCCGAAGCCGCCGGCCCTCCCGTTCCGCGTGGGCCACGGCTTCGA 5 0.125 No Hit GGGATGTTGTCATGCTGGATGATGACGAAGACTTGCATGATGCAGCTATC 5 0.125 No Hit CAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTGCTCAG 5 0.125 No Hit CAAAATTGCTTTATTGTCATTGACATCAATCCTGCAAATTCTACCATCAT 5 0.125 No Hit GCTTTTAAGTTCGCCCTGGATGCTGACTTCATTCTGACTTACACTGATGA 5 0.125 No Hit GCAAGTGCTTTTAAGTTCGCCCTGGATGCTGACTTCATTCTGACTTACAC 5 0.125 No Hit GTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTAT 5 0.125 No Hit AGATGGAGCAGGAGAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCACGA 10 0.007063091 143.3875 1 CACGACC 10 0.007063091 143.3875 3 GCACGAC 10 0.007063091 143.3875 2 CGACCAT 10 0.007063091 143.3875 5 >>END_MODULE Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082556 spots for ERR5262798.sra Written 2082556 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra Read 2082554 spots for ERR5262798.sra Written 2082554 spots for ERR5262798.sra SRR ids: ['ERR5262798.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0r_guxpf ERR5262798.sra spots: 41651082 blocks: [[1, 2082554], [2082555, 4165108], [4165109, 6247662], [6247663, 8330216], [8330217, 10412770], [10412771, 12495324], [12495325, 14577878], [14577879, 16660432], [16660433, 18742986], [18742987, 20825540], [20825541, 22908094], [22908095, 24990648], [24990649, 27073202], [27073203, 29155756], [29155757, 31238310], [31238311, 33320864], [33320865, 35403418], [35403419, 37485972], [37485973, 39568526], [39568527, 41651082]] ERR5262798 file size 13760707 ERR5262798 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262798 ERR5262798_1.fastq ERR5262798_2.fastq Input file: ERR5262798_1.fastq Paired file: ERR5262798_2.fastq trimmed: ERR5262798-trimmed-pair1.fastq, ERR5262798-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 02:13:33 2024 >> started Thu Dec 12 02:14:51 2024 >> done (78.549s) 41651082 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 41651082 (100.00%) read pairs available; of these: 10693 ( 0.03%) trimmed read pairs available after processing 41640389 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 0 0.00% 21 2 0.00% 22 0 0.00% 23 2 0.00% 24 1 0.00% 25 1 0.00% 26 0 0.00% 27 2 0.00% 28 2 0.00% 29 2 0.00% 30 1 0.00% 31 3 0.00% 32 3 0.00% 33 1 0.00% 34 1 0.00% 35 2 0.00% 36 2 0.00% 37 3 0.00% 38 3 0.00% 39 3 0.00% 40 0 0.00% 41 0 0.00% 42 1 0.00% 43 0 0.00% 44 1 0.00% 45 4 0.00% 46 2 0.00% 47 3 0.00% 48 1 0.00% 49 513 0.00% 50 474 0.00% 51 551 0.00% 52 581 0.00% 53 606 0.00% 54 594 0.00% 55 673 0.00% 56 786 0.00% 57 796 0.00% 58 915 0.00% 59 1027 0.00% 60 1107 0.00% 61 1271 0.00% 62 1401 0.00% 63 1508 0.00% 64 1566 0.00% 65 1751 0.00% 66 1890 0.00% 67 2165 0.01% 68 2383 0.01% 69 2684 0.01% 70 3122 0.01% 71 3441 0.01% 72 3825 0.01% 73 4261 0.01% 74 4788 0.01% 75 5199 0.01% 76 5592 0.01% 77 6185 0.01% 78 6818 0.02% 79 7763 0.02% 80 8587 0.02% 81 9677 0.02% 82 10744 0.03% 83 12154 0.03% 84 13214 0.03% 85 14351 0.03% 86 15074 0.04% 87 16425 0.04% 88 17659 0.04% 89 18390 0.04% 90 20448 0.05% 91 22538 0.05% 92 24509 0.06% 93 26400 0.06% 94 28615 0.07% 95 29754 0.07% 96 31747 0.08% 97 33640 0.08% 98 34357 0.08% 99 36057 0.09% 100 38511 0.09% 101 40358 0.10% 102 43514 0.10% 103 45791 0.11% 104 48168 0.12% 105 50472 0.12% 106 52894 0.13% 107 53607 0.13% 108 55900 0.13% 109 57928 0.14% 110 59301 0.14% 111 62793 0.15% 112 65689 0.16% 113 67721 0.16% 114 71666 0.17% 115 73922 0.18% 116 75865 0.18% 117 77518 0.19% 118 79764 0.19% 119 81392 0.20% 120 82519 0.20% 121 85531 0.21% 122 88063 0.21% 123 92272 0.22% 124 96072 0.23% 125 98476 0.24% 126 100356 0.24% 127 102033 0.24% 128 103358 0.25% 129 104833 0.25% 130 106851 0.26% 131 107994 0.26% 132 111662 0.27% 133 113593 0.27% 134 117366 0.28% 135 120481 0.29% 136 122732 0.29% 137 124418 0.30% 138 126598 0.30% 139 130603 0.31% 140 134783 0.32% 141 139053 0.33% 142 144046 0.35% 143 145957 0.35% 144 152711 0.37% 145 149431 0.36% 146 157277 0.38% 147 357379 0.86% 148 142078 0.34% 149 142318 0.34% 150 36146841 86.78% 41651082 reads passed initial QC criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=2.42 fanout-score-rank=45 prefix-density=0.20 prefix-fanout=2.3 sequence=CCCCAGTTAAGTGGGTCGGCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=45 fanout-score=182.77 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=7.9 sequence=CCATCACAAATCAAATGTTTAGACAGACAGATAACACCAGATAAAGCCACTCATGGCAGATCACAGGATAACAGCAATAGCAGGCCTTGGGATTGCAACAGGATACAGGCTCTAGTACTCATCGCCCTCATCGCCTTCATCACCCTCGTCGAACTCTGCACCAACCTCTTCATAATCCTTCTCCAGGGCAGCCAAATCCTCACGGGCCTCAGAGAACTCTCCCTCCTCCATGCCCTCACCCACATACCAGTGGACAAAGGCACGCTTGGCGTACATGAGATCAAACTTGTGGTCAATGCGGGAGAAGACCTCAACAACACTGGTGGAGTTGGAGATCATGCACACTGCCCTCTGGACCTTGGCAAGGTCACCACCTGGGACAACGCTAGGTGGCTGGTAGTTGATGCCACACTTGAAGCCAGTGGGGCACCAGTCAACAAA criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=17.27 fanout-score-rank=13 prefix-density=0.92 prefix-fanout=3.9 sequence=TCTTCTTCTTCCTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=13 fanout-score=180.75 fanout-score-rank=1 prefix-density=0.92 prefix-fanout=21.1 sequence=GCCGCCGCCGCAGCCGCCGACCGCAGCCATGTCTCTGATCGCGGGTGAGGAGTTCCAGCACATCCTGCGTCTGCTCAACACCAACGTCGATGGGAAGCAGAAGATCATGTTCGCGCTGACCTCCATCAAGGGTGTGGGCCGCCGCTTCTCCAACA ERR5262798 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 02:16:28 Started mapping on | Dec 12 02:16:28 Finished on | Dec 12 02:18:43 Mapping speed, Million of reads per hour | 1110.70 Number of input reads | 41651082 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 40186718 Uniquely mapped reads % | 96.48% Average mapped length | 293.10 Number of splices: Total | 36044512 Number of splices: Annotated (sjdb) | 33439988 Number of splices: GT/AG | 35566151 Number of splices: GC/AG | 414395 Number of splices: AT/AC | 25360 Number of splices: Non-canonical | 38606 Mismatch rate per base, % | 0.13% Deletion rate per base | 0.00% Deletion average length | 1.54 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 406916 % of reads mapped to multiple loci | 0.98% Number of reads mapped to too many loci | 1466 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.53% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1057448 1057448 1057448 N_multimapping 406916 406916 406916 N_noFeature 1524067 39112686 1881578 N_ambiguous 843767 6028 126828 UnstrandedReadsAssigned:37818884 PositiveStrandReadsAssigned:1068004 NegativeStrandReadsAssigned:38178312 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262798 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262798-trimmed-pair1.fastq ERR5262798-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 41,651,082 reads, 38,848,700 reads pseudoaligned [quant] estimated average fragment length: 278.599 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,248 rounds 52973 ERR5262798.ke.tsv 35125 ERR5262798.se.tsv 88098 total ==> ERR5262798.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 659.049 0 0 PNS24247 1044 766.401 208.453 10.1613 PNS24249 1928 1650.4 656.921 14.8703 PNS24246 1044 766.401 208.453 10.1613 PNS24248 1044 766.401 208.453 10.1613 PNS24244 1471 1193.4 239.718 7.50429 PNS24243 293 95.684 2 0.780882 KQK14069 1603 1325.4 49409.1 1392.69 KQK14071 474 230.463 106.731 17.3015 ==> ERR5262798.se.tsv <== BRADI_1g14170v3 49303 BRADI_1g53295v3 301 BRADI_1g59795v3 581 BRADI_1g07683v3 0 BRADI_1g00485v3 36 BRADI_1g20270v3 1452 BRADI_1g74790v3 2615 BRADI_1g09890v3 0 BRADI_1g77505v3 372 BRADI_1g48960v3 0 ERR5262798 completed mapping pipeline successfully