Starting /dee2/code/volunteer_pipeline.sh ERR5262799 current disk space = 1551346679808 free memory = 1600500448 ERR5262799 SRAfilesize b0de62f5f579e9560c76c06c03968c74 ERR5262799.sra ERR5262799.sra file validated ERR5262799 is paired end ERR5262799 is conventional basespace ERR5262799 read1 length is 81-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262799_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 81-150 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.474 37.0 37.0 37.0 37.0 37.0 2 36.3485 37.0 37.0 37.0 37.0 37.0 3 36.5585 37.0 37.0 37.0 37.0 37.0 4 36.634 37.0 37.0 37.0 37.0 37.0 5 36.5945 37.0 37.0 37.0 37.0 37.0 6 36.6135 37.0 37.0 37.0 37.0 37.0 7 36.596 37.0 37.0 37.0 37.0 37.0 8 36.6785 37.0 37.0 37.0 37.0 37.0 9 36.5145 37.0 37.0 37.0 37.0 37.0 10-14 36.66420000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.6082 37.0 37.0 37.0 37.0 37.0 20-24 36.5758 37.0 37.0 37.0 37.0 37.0 25-29 36.537699999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.598400000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.52610000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.54110000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.5168 37.0 37.0 37.0 37.0 37.0 50-54 36.5705 37.0 37.0 37.0 37.0 37.0 55-59 36.5461 37.0 37.0 37.0 37.0 37.0 60-64 36.5189 37.0 37.0 37.0 37.0 37.0 65-69 36.492599999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.4892 37.0 37.0 37.0 37.0 37.0 75-79 36.5113 37.0 37.0 37.0 37.0 37.0 80-84 36.49601942985746 37.0 37.0 37.0 37.0 37.0 85-89 36.50947937084321 37.0 37.0 37.0 37.0 37.0 90-94 36.46533466933667 37.0 37.0 37.0 37.0 37.0 95-99 36.41691277471727 37.0 37.0 37.0 37.0 37.0 100-104 36.42066730132656 37.0 37.0 37.0 37.0 37.0 105-109 36.48140533438478 37.0 37.0 37.0 37.0 37.0 110-114 36.393006376923246 37.0 37.0 37.0 37.0 37.0 115-119 36.241830698303204 37.0 37.0 37.0 37.0 37.0 120-124 36.217029526536045 37.0 37.0 37.0 37.0 37.0 125-129 36.30054912557317 37.0 37.0 37.0 37.0 37.0 130-134 36.29845756778339 37.0 37.0 37.0 37.0 37.0 135-139 36.20824644407206 37.0 37.0 37.0 37.0 37.0 140-144 36.00557077467191 37.0 37.0 37.0 37.0 37.0 145-149 36.151811961484086 37.0 37.0 37.0 37.0 37.0 150 36.206774876499644 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 27 3.0 28 8.0 29 5.0 30 8.0 31 21.0 32 25.0 33 71.0 34 103.0 35 298.0 36 2607.0 37 851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.525 30.8 6.800000000000001 22.875 2 23.925 9.225 32.9 33.95 3 16.675 18.65 19.225 45.45 4 18.925 38.375 17.75 24.95 5 21.9 42.699999999999996 14.6 20.8 6 21.325 40.025 23.05 15.6 7 12.8 27.750000000000004 42.275 17.175 8 25.900000000000002 23.724999999999998 24.349999999999998 26.025 9 19.575 18.175 38.725 23.525 10-14 18.655 31.945 29.28 20.119999999999997 15-19 20.474999999999998 27.62 25.405 26.5 20-24 25.355 28.599999999999998 24.765 21.279999999999998 25-29 18.955 30.745 28.535 21.765 30-34 23.345 26.915 26.875 22.865 35-39 18.64 23.01 27.3 31.05 40-44 20.29 27.284999999999997 25.81 26.615 45-49 26.915 25.695 23.89 23.5 50-54 27.139999999999997 24.205 23.919999999999998 24.735 55-59 24.125 25.995 22.79 27.089999999999996 60-64 23.785 24.19 28.125 23.9 65-69 22.165000000000003 21.955 28.52 27.36 70-74 21.32 20.645 24.255 33.78 75-79 22.515 22.86 23.735 30.89 80-84 27.344101615242288 25.52882932439866 22.84842726408961 24.27864179626944 85-89 28.037616927617425 19.463758691411133 23.27047171227052 29.228152668700915 90-94 26.1583108175723 21.424997498248775 21.87531271890323 30.541378965275694 95-99 21.564111550593303 18.78035347719421 30.751514544635256 28.904020427577233 100-104 17.140422118614328 20.038100967564045 28.796310222088533 34.02516669173309 105-109 22.225395556063287 19.54646712743474 25.538356086136975 32.68978123036499 110-114 26.870662460567825 20.164037854889592 24.9211356466877 28.04416403785489 115-119 19.09206349206349 20.965079365079365 27.68888888888889 32.25396825396825 120-124 21.941361390902674 18.213896195547573 23.320715981266442 36.524026432283314 125-129 21.506982786619034 17.661578434556674 25.384865215979215 35.44657356284508 130-134 25.75558475689882 14.829172141918528 30.75558475689882 28.65965834428384 135-139 24.096385542168676 22.91819210543833 22.046195833055982 30.939226519337016 140-144 22.483722192078133 17.586814975583287 22.938144329896907 36.99131850244167 145-149 23.391406954952455 17.137502602901368 24.800444228500034 34.670646213646144 150 15.913902611150318 13.091037402964009 36.87367678193366 34.12138320395201 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 1.0 27 2.0 28 4.0 29 9.5 30 13.5 31 10.0 32 7.5 33 6.0 34 13.5 35 34.5 36 42.0 37 63.0 38 61.0 39 55.0 40 77.5 41 154.5 42 166.0 43 144.5 44 211.5 45 235.0 46 178.0 47 164.5 48 282.0 49 228.0 50 80.5 51 70.0 52 70.0 53 75.5 54 89.5 55 112.0 56 145.0 57 413.5 58 370.0 59 92.5 60 117.5 61 72.5 62 23.0 63 15.0 64 7.5 65 6.0 66 8.5 67 13.0 68 10.5 69 4.5 70 5.5 71 7.5 72 4.5 73 4.0 74 7.5 75 4.5 76 0.5 77 0.0 78 1.0 79 1.0 80 0.0 81 1.5 82 1.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 80-81 1.0 82-83 0.0 84-85 1.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.0 94-95 1.0 96-97 1.0 98-99 1.0 100-101 4.0 102-103 5.0 104-105 2.0 106-107 802.0 108-109 7.0 110-111 0.0 112-113 10.0 114-115 7.0 116-117 14.0 118-119 3.0 120-121 22.0 122-123 20.0 124-125 9.0 126-127 18.0 128-129 7.0 130-131 20.0 132-133 19.0 134-135 12.0 136-137 17.0 138-139 29.0 140-141 16.0 142-143 22.0 144-145 30.0 146-147 34.0 148-149 30.0 150-151 2834.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.75 #Duplication Level Percentage of deduplicated Percentage of total 1 51.78947368421053 12.3 2 18.10526315789474 8.6 3 10.0 7.124999999999999 4 5.36842105263158 5.1 5 2.736842105263158 3.25 6 2.3157894736842106 3.3000000000000003 7 1.1578947368421053 1.925 8 1.263157894736842 2.4 9 0.42105263157894735 0.8999999999999999 >10 6.105263157894736 31.424999999999997 >50 0.42105263157894735 6.525 >100 0.3157894736842105 17.150000000000002 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCAAATGTTTGAACGATCGGGGAAATTCGAGCTCTCCGGATCCCGGTCG 441 11.025 No Hit GGAAATTCGAGCTCTCCGGATCCCGGTCGGCATCTACTCTATTCCTTTGC 132 3.3000000000000003 No Hit GTCCATCACAATGACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGT 113 2.825 No Hit CGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGT 69 1.725 No Hit GCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTT 68 1.7000000000000002 No Hit GCTCCTAGTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGAT 64 1.6 No Hit CCCGGTTTTATTATTATTATTGCTCCTAGTTGAAATTTACAAGCAAAGAT 60 1.5 No Hit GCCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCGCGGAA 50 1.25 No Hit GACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGGAGGCACAATTGTT 50 1.25 No Hit ATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAAT 44 1.0999999999999999 No Hit CATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAA 44 1.0999999999999999 No Hit CTCCGGATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTG 41 1.0250000000000001 No Hit GTTCCATCTAGAACTTATTCCAACTAATACCACATCACAATCACATACAA 39 0.975 No Hit CCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGGCGT 38 0.95 No Hit GGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCAGTTTGATG 37 0.9249999999999999 No Hit ATTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTCTCCGGATCCCGG 37 0.9249999999999999 No Hit GATCGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCAGTTT 36 0.8999999999999999 No Hit CCCGAACAGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGG 35 0.8750000000000001 No Hit CCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCGCGGAAA 34 0.8500000000000001 No Hit ATCACAATGACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGGA 33 0.8250000000000001 No Hit GGGAAATTCGAGCTCTCCGGATCCCGGTCGGCATCTACTCTATTCCTTTG 32 0.8 No Hit ACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGGAGGCACAATTGTTT 31 0.775 No Hit GGCTCGCCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCG 31 0.775 No Hit ATCGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCAGTTTG 29 0.7250000000000001 No Hit CCTAGTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGATACT 28 0.7000000000000001 No Hit GTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGATACTAAGT 27 0.675 No Hit GCCTTCTGTGACAAGGTAGGAGGCACAATTGTTTGCCCCTAGCCACAAGG 26 0.65 No Hit TCCTAGTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGATAC 25 0.625 No Hit CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACG 24 0.6 No Hit CTCCTAGTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGATA 20 0.5 No Hit GCAAAGATCTGATCGGTACATATATAGATACTAAGTAGAACGGTAGGACC 19 0.475 No Hit CAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTA 18 0.44999999999999996 No Hit CTTAAGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCT 18 0.44999999999999996 No Hit GCAAGAGCTCTTTCCCGAACAGCTCAGCGATGGCTCGCCCAGGATCTTCT 17 0.42500000000000004 No Hit ATCTGATCGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCA 17 0.42500000000000004 No Hit CCGGTTTTATTATTATTATTGCTCCTAGTTGAAATTTACAAGCAAAGATC 17 0.42500000000000004 No Hit CTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTA 17 0.42500000000000004 No Hit GTCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCGCGGAA 16 0.4 No Hit GTTCGAGGTTCCATCTAGAACTTATTCCAACTAATACCACATCACAATCA 16 0.4 No Hit CTAGAACTTATTCCAACTAATACCACATCACAATCACATACAACGCCGCA 15 0.375 No Hit CTTTGTCCATCACAATGACTGACGAAATTTACAAATTGCCTTCTGTGACA 15 0.375 No Hit CCATCACAATGACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGTAG 15 0.375 No Hit GCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGGACTCTCCT 14 0.35000000000000003 No Hit GTCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGG 14 0.35000000000000003 No Hit GCTCGCCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCGC 13 0.325 No Hit CCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGGC 13 0.325 No Hit GATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATT 13 0.325 No Hit GCTCTCCGGATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGA 13 0.325 No Hit CGAACAGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGGAC 13 0.325 No Hit CTAGTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGATACTA 13 0.325 No Hit CCGGATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCT 12 0.3 No Hit TCTTTGTCCATCACAATGACTGACGAAATTTACAAATTGCCTTCTGTGAC 12 0.3 No Hit CGGTTTTATTATTATTATTGCTCCTAGTTGAAATTTACAAGCAAAGATCT 12 0.3 No Hit CGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCAGTTTGAT 11 0.27499999999999997 No Hit GAGCTCTTTCCCGAACAGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCG 11 0.27499999999999997 No Hit CCATCTAGAACTTATTCCAACTAATACCACATCACAATCACATACAACGC 11 0.27499999999999997 No Hit TTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTCTCCGGATCCCGGT 11 0.27499999999999997 No Hit TGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTAT 10 0.25 No Hit CGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGGCGTC 10 0.25 No Hit AGCAAAGATCTGATCGGTACATATATAGATACTAAGTAGAACGGTAGGAC 10 0.25 No Hit CTCGCCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCGCG 10 0.25 No Hit ACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAAC 10 0.25 No Hit AGGTAACCCGGTTTTATTATTATTATTGCTCCTAGTTGAAATTTACAAGC 10 0.25 No Hit GACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGGAGGCACAAT 10 0.25 No Hit CTCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCGCGGAAA 10 0.25 No Hit GAGCTCTCCGGATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGAC 9 0.22499999999999998 No Hit ATCTAGAACTTATTCCAACTAATACCACATCACAATCACATACAACGCCG 9 0.22499999999999998 No Hit CTCTCCGGATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAG 9 0.22499999999999998 No Hit GAACAGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGGACT 9 0.22499999999999998 No Hit TTAAGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTC 8 0.2 No Hit ATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCA 8 0.2 No Hit TTTTTTTTTTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATCG 8 0.2 No Hit ATCCCGAACAGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAG 8 0.2 No Hit GAACGCTCTACGCCTTATATCCGACTGACTGCCCAGTGCATCACATGGCA 8 0.2 No Hit CCCCGAACAGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGG 8 0.2 No Hit TTTTTTTTTTTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATC 8 0.2 No Hit CCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGGCG 8 0.2 No Hit GGCATCCATCCCAGCAAGTGAACGCTCTACGCCTTATATCCGACTGACTG 8 0.2 No Hit TTTTTTTTTTTTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGAT 8 0.2 No Hit CCCTAGTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGATAC 8 0.2 No Hit GTCGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCAGTTTG 8 0.2 No Hit AGCACCTGGGCCTGGGTGCTGCTGGTGGTGCTGGCCTGCTCTGCCAGGTC 7 0.17500000000000002 No Hit TCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATG 7 0.17500000000000002 No Hit ATGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTA 7 0.17500000000000002 No Hit GATCTGATCGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCC 7 0.17500000000000002 No Hit CATCACAATGACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGG 7 0.17500000000000002 No Hit CTTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTCTCCGGATCCCGG 7 0.17500000000000002 No Hit GGTTTTATTATTATTATTGCTCCTAGTTGAAATTTACAAGCAAAGATCTG 7 0.17500000000000002 No Hit GCTTCTAGTTGAAATTTACAAGCAAAGATCTGATCGGTACATATATAGAT 7 0.17500000000000002 No Hit AGAACTTATTCCAACTAATACCACATCACAATCACATACAACGCCGCAGG 7 0.17500000000000002 No Hit AAGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTCTC 7 0.17500000000000002 No Hit CCGAACAGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGGA 7 0.17500000000000002 No Hit GGACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGGAGGCACAA 6 0.15 No Hit AGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAA 6 0.15 No Hit ATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAAAT 6 0.15 No Hit AAGCAAAGATCTGATCGGTACATATATAGATACTAAGTAGAACGGTAGGA 6 0.15 No Hit GTCCATCTAGAACTTATTCCAACTAATACCACATCACAATCACATACAAC 6 0.15 No Hit GTCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAAT 6 0.15 No Hit GATACAGGTAACCCGGTTTTATTATTATTATTGCTCCTAGTTGAAATTTA 6 0.15 No Hit AGCTCAGCGATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGGACTCTCC 6 0.15 No Hit AATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCC 6 0.15 No Hit GCTTATAATTACGAGAAGAAATGGAGGAAAAAAAAGAAAATGCTTCCCAA 6 0.15 No Hit CTGATCGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCAGT 6 0.15 No Hit AGCACTTCGGCCAATACCCCACGGCATTCACCTCTGCAAGGATTCCCATG 6 0.15 No Hit GTTACATCTTCAATTAAGAGAATTCGGATACTAAAGTACTTGCTTATAAT 6 0.15 No Hit AACAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCAATCT 6 0.15 No Hit GGATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGG 6 0.15 No Hit CAGGTAACCCGGTTTTATTATTATTATTGCTCCTAGTTGAAATTTACAAG 6 0.15 No Hit GGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAA 6 0.15 No Hit GATGGCTCGCCCAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATA 6 0.15 No Hit TTTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTCTCCGGATCCCGG 6 0.15 No Hit CATCTAGAACTTATTCCAACTAATACCACATCACAATCACATACAACGCC 6 0.15 No Hit GCCCAGTGCATCACATGGCATCTATAGTCAGGGCAATTGGTTTCTGTACA 6 0.15 No Hit ATTCGAGCTCTCCGGATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTC 6 0.15 No Hit CGAGGTTCCATCTAGAACTTATTCCAACTAATACCACATCACAATCACAT 5 0.125 No Hit CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA 5 0.125 No Hit AGATAGGAGATCTACAAATGGGTTAAAATGCCAACACATTTGCAATTTTA 5 0.125 No Hit ATCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGG 5 0.125 No Hit CATCCATCCCATCCCAGCAAGTGAACACTGAATCATCTGGCATCCATCCC 5 0.125 No Hit CGACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGGAGGCACAA 5 0.125 No Hit TAGAACTTATTCCAACTAATACCACATCACAATCACATACAACGCCGCAG 5 0.125 No Hit ATCCCATCCCAGCAAGTGAACACTGAATCATCTGGCATCCATCCCAGCAA 5 0.125 No Hit CAGCAAGTGAACACTGAATCATCTGGCATCCATCCCAGCAAGTGAACGCT 5 0.125 No Hit CAAAGATCTGATCGGTACATATATAGATACTAAGTAGAACGGTAGGACCA 5 0.125 No Hit CTGGCATCCATCCCAGCAAGTGAACGCTCTACGCCTTATATCCGACTGAC 5 0.125 No Hit ATGTAGGGCTCCACTACCAGCGGCCTGCCGGAGATGAGCACCACCACGCA 5 0.125 No Hit CCAGGATCTTCTTCCGTGATTAGGGAATCTCCTACCAATACCGCGGAAAC 5 0.125 No Hit GTTTTATTATTATTATTGCTCCTAGTTGAAATTTACAAGCAAAGATCTGA 5 0.125 No Hit CCATCCCATCCCAGCAAGTGAACACTGAATCATCTGGCATCCATCCCAGC 5 0.125 No Hit GCCCGGTCGGCATCTACTCTATTCCTTTGCCCTCGGACGAGTGCTGGGGC 5 0.125 No Hit GCCTAGGATCTTCTTCCGTGATTAGGGACTCTCCTACCAATACCGCGGAA 5 0.125 No Hit GTTCCGTCTAGAACTTATTCCAACTAATACCACATCACAATCACATACAA 5 0.125 No Hit AGATGAAGGTGGAGGCGTCTGAGAGGACCTGCTTCGAGTAATCGAGGCCG 5 0.125 No Hit TGACTGACGAAATTTACAAATTGCCTTCTGTGACAAGGTAGGAGGCACAA 5 0.125 No Hit GGTCGGTACATATATAGATACTAAGTAGAACGGTAGGACCAGCCCAGTTT 5 0.125 No Hit AGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTCTCC 5 0.125 No Hit GCCTTATATCCGACTGACTGCCCAGTGCATCACATGGCATCTATAGTCAG 5 0.125 No Hit ATTGCCTTCTGTGACAAGGTAGGAGGCACAATTGTTTGCCCCTAGCCACA 5 0.125 No Hit AGAGCTTGATTCGGTCCCCGAATAGCCCGAACACCAGGTCCGCGTACTCC 5 0.125 No Hit CCCAGCAAGTGAACGCTCTACGCCTTATATCCGACTGACTGCCCAGTGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGAC 20 4.984019E-4 100.125 9 CACAATG 20 4.984019E-4 100.125 7 CCATCAC 20 4.984019E-4 100.125 3 GTCCATC 20 4.984019E-4 100.125 1 TCCATCA 20 4.984019E-4 100.125 2 ACAATGA 20 4.984019E-4 100.125 8 GTTTGAA 105 2.0008883E-11 57.214287 8 CCAAATG 105 2.0008883E-11 57.214287 2 TGTTTGA 105 2.0008883E-11 57.214287 7 ATGTTTG 105 2.0008883E-11 57.214287 6 GCCAAAT 105 2.0008883E-11 57.214287 1 AATGTTT 105 2.0008883E-11 57.214287 5 CAAATGT 105 2.0008883E-11 57.214287 3 AAATGTT 105 2.0008883E-11 57.214287 4 ATCACAA 35 0.004595378 57.214283 5 TCACAAT 35 0.004595378 57.214283 6 TTTGAAC 110 3.274181E-11 54.613636 9 CATCACA 40 0.007795063 50.0625 4 CGGCCGC 90 6.6876964E-8 20.892017 140-144 GCCGCGC 90 6.6876964E-8 20.892017 140-144 >>END_MODULE ERR5262799 read2 length is 81-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262799_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 81-150 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9825 37.0 37.0 37.0 37.0 37.0 2 36.038 37.0 37.0 37.0 37.0 37.0 3 36.115 37.0 37.0 37.0 37.0 37.0 4 36.048 37.0 37.0 37.0 37.0 37.0 5 36.111 37.0 37.0 37.0 37.0 37.0 6 36.0355 37.0 37.0 37.0 37.0 37.0 7 36.1255 37.0 37.0 37.0 37.0 37.0 8 36.1535 37.0 37.0 37.0 37.0 37.0 9 36.211 37.0 37.0 37.0 37.0 37.0 10-14 36.207899999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.161699999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.207899999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.164500000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.1453 37.0 37.0 37.0 37.0 37.0 35-39 36.151599999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.1182 37.0 37.0 37.0 37.0 37.0 45-49 36.1089 37.0 37.0 37.0 37.0 37.0 50-54 36.1014 37.0 37.0 37.0 37.0 37.0 55-59 35.9928 37.0 37.0 37.0 37.0 37.0 60-64 36.0415 37.0 37.0 37.0 37.0 37.0 65-69 36.013600000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.0476 37.0 37.0 37.0 37.0 37.0 75-79 36.03529999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.87681860465116 37.0 37.0 37.0 37.0 37.0 85-89 35.911875949585195 37.0 37.0 37.0 37.0 37.0 90-94 35.901983346071525 37.0 37.0 37.0 37.0 37.0 95-99 35.931500184176386 37.0 37.0 37.0 37.0 37.0 100-104 35.85723108769126 37.0 37.0 37.0 37.0 37.0 105-109 35.80072084959181 37.0 37.0 37.0 37.0 37.0 110-114 35.72254699429444 37.0 37.0 37.0 37.0 37.0 115-119 35.790510530398045 37.0 37.0 37.0 37.0 37.0 120-124 35.74837657423399 37.0 37.0 37.0 37.0 37.0 125-129 35.71121034681658 37.0 37.0 37.0 37.0 37.0 130-134 35.703523173164015 37.0 37.0 37.0 37.0 37.0 135-139 35.70643560462971 37.0 37.0 37.0 37.0 37.0 140-144 35.56822706054987 37.0 37.0 37.0 37.0 37.0 145-149 35.55355208385692 37.0 37.0 37.0 37.0 37.0 150 35.62286931818182 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 5.0 13 1.0 14 2.0 15 2.0 16 1.0 17 1.0 18 1.0 19 3.0 20 1.0 21 8.0 22 7.0 23 10.0 24 5.0 25 5.0 26 11.0 27 5.0 28 10.0 29 12.0 30 14.0 31 30.0 32 46.0 33 57.0 34 152.0 35 450.0 36 2782.0 37 373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.575 18.3 7.000000000000001 27.125 2 36.775000000000006 17.349999999999998 22.225 23.65 3 29.849999999999998 23.474999999999998 23.25 23.425 4 32.25 26.3 17.65 23.799999999999997 5 29.75 30.925000000000004 16.75 22.575 6 28.075 33.0 18.725 20.200000000000003 7 24.875 17.575 30.95 26.6 8 24.325 21.55 23.075000000000003 31.05 9 30.925000000000004 19.075 23.474999999999998 26.525 10-14 31.259999999999998 22.384999999999998 19.49 26.865 15-19 29.845 25.069999999999997 20.07 25.014999999999997 20-24 29.635 23.255 20.575 26.534999999999997 25-29 30.595 20.745 20.035 28.625 30-34 31.064999999999998 22.695 20.25 25.990000000000002 35-39 30.34 24.04 20.62 25.0 40-44 30.72 22.035 21.29 25.955000000000002 45-49 31.14 22.425 21.465 24.97 50-54 30.490000000000002 23.1 21.05 25.36 55-59 30.335 22.555 21.745 25.365 60-64 30.36 22.275 22.085 25.28 65-69 29.134999999999998 25.645 21.375 23.845 70-74 29.65 22.505 21.375 26.47 75-79 30.23 23.215 21.695 24.86 80-84 29.684452667900185 23.58853828074211 21.278191728759314 25.448817322598387 85-89 29.068894781608044 24.290789012858358 21.569019862910892 25.071296342622706 90-94 28.504906869617464 23.6981774484278 21.835569797716804 25.961345884237936 95-99 29.24873452613642 24.462486844083596 22.277351776675186 24.011426853104798 100-104 28.851849992469504 22.742105527385913 22.947939153571966 25.458105326572618 105-109 26.38920649439744 24.971415504230503 23.885204664989708 24.754173336382344 110-114 31.810725552050474 24.126182965299684 21.230283911671926 22.832807570977916 115-119 31.166603126191383 26.127843436268904 19.837336383276146 22.868217054263564 120-124 29.96329448129306 24.88891750917638 20.86418958078434 24.283598428746217 125-129 28.368886576482833 24.72684703433923 21.344953173777313 25.559313215400625 130-134 29.39784380752038 23.45516697344202 21.732842492768867 25.414146726268733 135-139 28.263337116912602 22.354276557387994 22.087200373906658 27.29518595179275 140-144 30.065493246009005 24.08923454768727 21.21026060854141 24.635011597762315 145-149 30.820383592328156 26.571468570628586 20.33459330813384 22.273554528909422 150 29.296875 26.49147727272727 21.448863636363637 22.76278409090909 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 0.5 25 0.0 26 0.0 27 0.5 28 0.5 29 0.0 30 0.0 31 0.5 32 0.5 33 0.5 34 4.5 35 10.5 36 10.5 37 18.0 38 21.5 39 19.5 40 31.5 41 38.5 42 43.0 43 74.0 44 76.0 45 69.0 46 87.5 47 123.5 48 167.0 49 158.0 50 153.0 51 162.0 52 140.0 53 123.0 54 134.5 55 113.5 56 114.5 57 159.5 58 170.5 59 167.0 60 163.0 61 164.0 62 170.0 63 217.0 64 273.0 65 215.5 66 105.5 67 73.0 68 62.0 69 50.5 70 40.5 71 21.5 72 16.5 73 8.0 74 6.5 75 6.0 76 2.5 77 2.0 78 1.0 79 1.0 80 2.5 81 2.5 82 0.5 83 0.0 84 0.0 85 0.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 80-81 1.0 82-83 0.0 84-85 1.0 86-87 1.0 88-89 1.0 90-91 0.0 92-93 4.0 94-95 1.0 96-97 1.0 98-99 1.0 100-101 6.0 102-103 6.0 104-105 2.0 106-107 795.0 108-109 7.0 110-111 0.0 112-113 10.0 114-115 9.0 116-117 14.0 118-119 7.0 120-121 31.0 122-123 18.0 124-125 4.0 126-127 10.0 128-129 7.0 130-131 21.0 132-133 22.0 134-135 17.0 136-137 18.0 138-139 32.0 140-141 16.0 142-143 33.0 144-145 34.0 146-147 26.0 148-149 28.0 150-151 2816.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.35 #Duplication Level Percentage of deduplicated Percentage of total 1 53.494282083862764 21.05 2 17.53494282083863 13.8 3 10.48284625158831 12.375 4 5.972045743329097 9.4 5 3.6848792884371027 7.249999999999999 6 2.2236340533672174 5.25 7 1.4612452350698857 4.025 8 1.207115628970775 3.8 9 0.7623888182973316 2.7 >10 3.176620076238882 20.349999999999998 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACA 44 1.0999999999999999 No Hit CGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCG 39 0.975 No Hit AGTTAAGCAAGTCAGGAAAGCGAACGCAGCATTGTACATCAAACTGGGCT 31 0.775 No Hit GGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACC 30 0.75 No Hit GTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGA 29 0.7250000000000001 No Hit CACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAA 27 0.675 No Hit GCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATC 26 0.65 No Hit CGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCT 25 0.625 No Hit GCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGG 22 0.5499999999999999 No Hit GCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGC 21 0.525 No Hit GTGATCTTCACGTGGAAATTCAGCATTGGCCGGGACAAGAATTTCCAACA 19 0.475 No Hit GGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGAC 19 0.475 No Hit GGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGT 19 0.475 No Hit GGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTAC 18 0.44999999999999996 No Hit CCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGG 18 0.44999999999999996 No Hit CGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGA 18 0.44999999999999996 No Hit GGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTA 17 0.42500000000000004 No Hit GCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTG 16 0.4 No Hit CGGTGATAGCCGGCGCAGATTTCGGTCCTTGGAGTGAAGCATTGGAAGCG 15 0.375 No Hit AGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGT 14 0.35000000000000003 No Hit GTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGG 13 0.325 No Hit GGATCGGCTCATTGGCCAGCCCTTGCGCGGCCTTGCCAGACTGGGATTTC 13 0.325 No Hit ATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAA 13 0.325 No Hit GGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGT 13 0.325 No Hit GGCGAATCTGGGCTGTTCACTCCGGATGATGTTGCATATGTGCAGAGTGC 13 0.325 No Hit GGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGT 13 0.325 No Hit AGGAGGCCGAGAAGACAGAGGACGTGCTCTACTCCGACCGCGTCGCGATC 13 0.325 No Hit GCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGAC 12 0.3 No Hit CTTCAAACACGCGGATGTTGCTGGAGAAACGTGGTAATGTCATGAGGGAG 12 0.3 No Hit CCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGG 12 0.3 No Hit GGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGATG 12 0.3 No Hit ATCAAATTCGCCATCCCCAGATAGCCTTCCTGCCGGAACTGGTGATCTTC 12 0.3 No Hit GCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTC 12 0.3 No Hit GCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGA 12 0.3 No Hit GGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCC 12 0.3 No Hit CAAGAATTTCCAACAAGAGGAGGGGGGGAGTTAAGCAAGTCAGGAAAGCG 11 0.27499999999999997 No Hit ACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAG 11 0.27499999999999997 No Hit GTGATAGCCGGCGCAGATTTCGGTCCTTGGAGTGAAGCATTGGAAGCGGA 11 0.27499999999999997 No Hit CTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGT 11 0.27499999999999997 No Hit GGTGATCTTCACGTGGAAATTCAGCATTGGCCGGGACAAGAATTTCCAAC 11 0.27499999999999997 No Hit GCATTGGAAGCGGAGGTTGATGTCCAGTCAGTTGATGTGGACGCTGTGCC 11 0.27499999999999997 No Hit ATTCAGCATTGGCCGGGACAAGAATTTCCAACAAGAGGAGGGGGGGAGTT 11 0.27499999999999997 No Hit AGACATTTGAAGTGGATACTTCAAACACGCGGATGTTGCTGGAGAAACGT 11 0.27499999999999997 No Hit GGCGCAGATCACGCGCTGGAGAGCATCCACAACATCAGGGAGGCGCTGCC 11 0.27499999999999997 No Hit GTTGAGCTTATCGGCATCAACAACCGCAGTCTAGAGACATTTGAAGTGGA 11 0.27499999999999997 No Hit GATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGC 10 0.25 No Hit GCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATT 10 0.25 No Hit CAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGT 10 0.25 No Hit ATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATT 10 0.25 No Hit GGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGC 10 0.25 No Hit ACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGC 9 0.22499999999999998 No Hit GCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGAT 9 0.22499999999999998 No Hit GGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTG 9 0.22499999999999998 No Hit GGGGATACTGGTTGTTGGCGAATCTGGGCTGTTCACTCCGGATGATGTTG 9 0.22499999999999998 No Hit GGAAGCGAATTGGTCTCCACACAGTGCTGGTTGGCACGTCGCACCGGGTG 9 0.22499999999999998 No Hit GCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGC 9 0.22499999999999998 No Hit GACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTG 9 0.22499999999999998 No Hit CGGAGGTTGATGTCCAGTCAGTTGATGTGGACGCTGTGCCAACTGTACAA 9 0.22499999999999998 No Hit CGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGT 9 0.22499999999999998 No Hit GCCTTCCTGCCGGAACTGGTGATCTTCACGTGGAAATTCAGCATTGGCCG 9 0.22499999999999998 No Hit CGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCG 9 0.22499999999999998 No Hit CGTCGCGATCGAGACCGCGGTGACCGCGTAGGCTGATCTCCCGGTGCGCC 9 0.22499999999999998 No Hit CTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCG 8 0.2 No Hit GCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGC 8 0.2 No Hit GGGATACTGGTTGTTGGCGAATCTGGGCTGTTCACTCCGGATGATGTTGC 8 0.2 No Hit GGGAGGAGGCCGAGAAGACAGAGGACGTGCTCTACTCCGACCGCGTCGCG 8 0.2 No Hit AGGACGTGCTCTACTCCGACCGCGTCGCGATCGAGACCGCGGTGACCGCG 8 0.2 No Hit GCCGTCGGTCTGAGATATGACCCCAAAACACACGACCCGATCAAATTCGC 8 0.2 No Hit CCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCA 8 0.2 No Hit GAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAA 8 0.2 No Hit CCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCG 8 0.2 No Hit CGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCG 8 0.2 No Hit AAGCATTGGAAGCGGAGGTTGATGTCCAGTCAGTTGATGTGGACGCTGTG 8 0.2 No Hit GAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGT 8 0.2 No Hit CAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCG 8 0.2 No Hit AGAAGACAGAGGACGTGCTCTACTCCGACCGCGTCGCGATCGAGACCGCG 8 0.2 No Hit GTGGACGCTGTGCCAACTGTACAAGGAGCACTTGATTTCGTTGAGTACGA 8 0.2 No Hit ATTGGAAGCGGAGGTTGATGTCCAGTCAGTTGATGTGGACGCTGTGCCAA 8 0.2 No Hit GGAAGATGAAACAGAGGATATGCTGAGAAGCGCGACCGAAAGGATCGGCT 8 0.2 No Hit GCTGGAGAAACGTGGTAATGTCATGAGGGAGAAGGGGATACTGGTTGTTG 8 0.2 No Hit GTCCAGTCAGTTGATGTGGACGCTGTGCCAACTGTACAAGGAGCACTTGA 8 0.2 No Hit GTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGC 7 0.17500000000000002 No Hit GATCAAATTCGCCATCCCCAGATAGCCTTCCTGCCGGAACTGGTGATCTT 7 0.17500000000000002 No Hit TGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACC 7 0.17500000000000002 No Hit GCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCA 7 0.17500000000000002 No Hit GAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATC 7 0.17500000000000002 No Hit GTGCCAACTGTACAAGGAGCACTTGATTTCGTTGAGTACGATAGATATCT 7 0.17500000000000002 No Hit AGCGAACGCTCTGAAGCTTCTACAGCAACAACGACGGCCACGGTGATAGC 7 0.17500000000000002 No Hit GTCATGAGGGAGAAGGGGATACTGGTTGTTGGCGAATCTGGGCTGTTCAC 7 0.17500000000000002 No Hit GGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGG 7 0.17500000000000002 No Hit CCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCC 7 0.17500000000000002 No Hit GATCTTGACATGAAGTACATGCTTCGCGTCTGCAGAAGTCTTGGAATGAC 7 0.17500000000000002 No Hit AGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGG 7 0.17500000000000002 No Hit TGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTT 7 0.17500000000000002 No Hit CGTCGCACCGGGTGAAGGGCGCAGATCACGCGCTGGAGAGCATCCACAAC 7 0.17500000000000002 No Hit CGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCT 7 0.17500000000000002 No Hit TGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGAT 7 0.17500000000000002 No Hit GACAAGAATTTCCAACAAGAGGAGGGGGGGAGTTAAGCAAGTCAGGAAAG 7 0.17500000000000002 No Hit GCTGATCTCCCGGTGCGCCGTCGGTCTGAGATATGACCCCAAAACACACG 7 0.17500000000000002 No Hit GTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGT 7 0.17500000000000002 No Hit TCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGC 7 0.17500000000000002 No Hit GTGAAGCATTGGAAGCGGAGGTTGATGTCCAGTCAGTTGATGTGGACGCT 7 0.17500000000000002 No Hit GTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAG 7 0.17500000000000002 No Hit GCTGTGGATCAAGCTGCAACTGTGGCTCAAACTGCACTTGCGGGAAGATG 7 0.17500000000000002 No Hit GGTTGATGTCCAGTCAGTTGATGTGGACGCTGTGCCAACTGTACAAGGAG 6 0.15 No Hit GCCCGATCTTGACATGAAGTACATGCTTCGCGTCTGCAGAAGTCTTGGAA 6 0.15 No Hit ACGTGGTAATGTCATGAGGGAGAAGGGGATACTGGTTGTTGGCGAATCTG 6 0.15 No Hit AGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGA 6 0.15 No Hit GGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTG 6 0.15 No Hit GGGAAACTTAGGCTGGGTAGCAGGAAGGCTCCTCCGTCGAAGAAGCCTCT 6 0.15 No Hit AGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAAC 6 0.15 No Hit GGAGGTTGATGTCCAGTCAGTTGATGTGGACGCTGTGCCAACTGTACAAG 6 0.15 No Hit GCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATC 6 0.15 No Hit ACGTGCTCTACTCCGACCGCGTCGCGATCGAGACCGCGGTGACCGCGTAG 6 0.15 No Hit ATTTCGGTACCGAACCGGAGGGTCCCTCCTTGGCGCATGAGATGAGATAT 6 0.15 No Hit CGAGAAGACAGAGGACGTGCTCTACTCCGACCGCGTCGCGATCGAGACCG 6 0.15 No Hit GCGTCGCGATCGAGACCGCGGTGACCGCGTAGGCTGATCTCCCGGTGCGC 6 0.15 No Hit ATCAGATTAGTTTCAGCACCGTCCGGGACAAAATAAGGGCTAAAGTTGGC 6 0.15 No Hit AAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTG 6 0.15 No Hit GGACAAGAATTTCCAACAAGAGGAGGGGGGGAGTTAAGCAAGTCAGGAAA 6 0.15 No Hit GCCAGACTGGGATTTCGAGGATGGCTGGATCGAGGTGCACAGCGAACGCT 6 0.15 No Hit AGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGG 6 0.15 No Hit ATTCGCCATCCCCAGATAGCCTTCCTGCCGGAACTGGTGATCTTCACGTG 6 0.15 No Hit CTCTTATTGAGGTTCATGACGAAAGAGAACTGGATCGCGTGCTGAAAATA 6 0.15 No Hit CAGCATTGGCCGGGACAAGAATTTCCAACAAGAGGAGGGGGGGAGTTAAG 6 0.15 No Hit AGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCG 6 0.15 No Hit GGATCAGATTAGTTTCAGCACCGTCCGGGACAAAATAAGGGCTAAAGTTG 6 0.15 No Hit ATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGT 6 0.15 No Hit GCTGAAAATAGATGGCGTTGAGCTTATCGGCATCAACAACCGCAGTCTAG 6 0.15 No Hit GCAACGATGAAGAGCAGCACGCTCACGGCGATCCTAGTTCTCCAGGCCAT 6 0.15 No Hit AAATAGATGGCGTTGAGCTTATCGGCATCAACAACCGCAGTCTAGAGACA 6 0.15 No Hit TGAGGTTCATGACGAAAGAGAACTGGATCGCGTGCTGAAAATAGATGGCG 6 0.15 No Hit GCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGT 6 0.15 No Hit ATCGAGACCGCGGTGACCGCGTAGGCTGATCTCCCGGTGCGCCGTCGGTC 6 0.15 No Hit CAGCGAACGCTCTGAAGCTTCTACAGCAACAACGACGGCCACGGTGATAG 6 0.15 No Hit ATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGA 6 0.15 No Hit CTTGATTTCGTTGAGTACGATAGATATCTGGGTGTCCTTGTGGCTAGGGG 6 0.15 No Hit CTGAGATATGACCCCAAAACACACGACCCGATCAAATTCGCCATCCCCAG 6 0.15 No Hit GCGGAGGTTGATGTCCAGTCAGTTGATGTGGACGCTGTGCCAACTGTACA 6 0.15 No Hit GCTGAGAAGCGCGACCGAAAGGATCGGCTCATTGGCCAGCCCTTGCGCGG 5 0.125 No Hit GTCTGCAGAAGTCTTGGAATGACAGCTCTTATTGAGGTTCATGACGAAAG 5 0.125 No Hit AGCAGATCTCGGGAAGTCCCTGGTGGAGGCGTATGAGGAGGACGAGGCGG 5 0.125 No Hit GCAACATCCAGGCCGGGAAGCGAATTGGTCTCCACACAGTGCTGGTTGGC 5 0.125 No Hit CATCAGGGAGGCGCTGCCAGAGCTGTGGGAGGAGGCCGAGAAGACAGAGG 5 0.125 No Hit GGCCGAGAAGACAGAGGACGTGCTCTACTCCGACCGCGTCGCGATCGAGA 5 0.125 No Hit CAGGAAGGCTCCTCCGTCGAAGAAGCCTCTATTGAAGCGAAAAAAGCGGA 5 0.125 No Hit TGATAGCCGGCGCAGATTTCGGTCCTTGGAGTGAAGCATTGGAAGCGGAG 5 0.125 No Hit GACGCATCCAACACGGCGTCGAGCACTGCAGGAGGGCAGCGGTTCAACCA 5 0.125 No Hit CCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGC 5 0.125 No Hit CTCACATGTTGCAGATCGATGTTGACACTGAGAAAGGAGGACTGACTGTC 5 0.125 No Hit GGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTC 5 0.125 No Hit GGCTCATTGGCCAGCCCTTGCGCGGCCTTGCCAGACTGGGATTTCGAGGA 5 0.125 No Hit CGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACA 5 0.125 No Hit CTGCACCTCTGACATATGGATCTGATTAAGATAATGGATTTGGGACACAG 5 0.125 No Hit CCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGATGCCGACCGGGATCC 5 0.125 No Hit CAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCG 5 0.125 No Hit GTACAAGGAGCACTTGATTTCGTTGAGTACGATAGATATCTGGGTGTCCT 5 0.125 No Hit CACGACCCGATCAAATTCGCCATCCCCAGATAGCCTTCCTGCCGGAACTG 5 0.125 No Hit GAAGTTTAGTTCCATTGATTTTTTGGCAATAGGTTCAGAATATGTTCCTC 5 0.125 No Hit GACGAAAGAGAACTGGATCGCGTGCTGAAAATAGATGGCGTTGAGCTTAT 5 0.125 No Hit GTACATGCTTCGCGTCTGCAGAAGTCTTGGAATGACAGCTCTTATTGAGG 5 0.125 No Hit TGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGA 5 0.125 No Hit CAGCAACAACGACGGCCACGGTGATAGCCGGCGCAGATTTCGGTCCTTGG 5 0.125 No Hit CCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACG 5 0.125 No Hit CTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGA 5 0.125 No Hit GCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGC 5 0.125 No Hit CAACAACCGCAGTCTAGAGACATTTGAAGTGGATACTTCAAACACGCGGA 5 0.125 No Hit CACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGC 5 0.125 No Hit CGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCG 5 0.125 No Hit GCCCTGGAGGACAAGTACGGGAGCTTCCTCAGCGACAAGATCATCAAGGA 5 0.125 No Hit GTTGGCTGGATGCCCCAAATGTTCCTAGACTGTGAAAGGCGCAATTTCGT 5 0.125 No Hit ATTTGAAGTGGATACTTCAAACACGCGGATGTTGCTGGAGAAACGTGGTA 5 0.125 No Hit TTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTC 5 0.125 No Hit GAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGAT 5 0.125 No Hit AGAATTTCCAACAAGAGGAGGGGGGGAGTTAAGCAAGTCAGGAAAGCGAA 5 0.125 No Hit CTTGGCGCATGAGATGAGATATCCGGGTGGGGACTGCACCTCTGACATAT 5 0.125 No Hit GTTAAGCAAGTCAGGAAAGCGAACGCAGCATTGTACATCAAACTGGGCTG 5 0.125 No Hit CGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGG 5 0.125 No Hit TGACAATCCCGGCGCCTGGCCCTTCGGTGATCCAGACCGTCTGCAAGAGC 5 0.125 No Hit GGAGGACTGACTGTCAACCCCAACTTCTTTGTGGATTTCGGTACCGAACC 5 0.125 No Hit AATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCA 5 0.125 No Hit GACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGC 5 0.125 No Hit GGATACTGGTTGTTGGCGAATCTGGGCTGTTCACTCCGGATGATGTTGCA 5 0.125 No Hit AGAGGATATGCTGAGAAGCGCGACCGAAAGGATCGGCTCATTGGCCAGCC 5 0.125 No Hit ACAACATCAGGGAGGCGCTGCCAGAGCTGTGGGAGGAGGCCGAGAAGACA 5 0.125 No Hit GGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCG 5 0.125 No Hit GCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAG 5 0.125 No Hit GCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTC 5 0.125 No Hit GCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCA 5 0.125 No Hit CAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCC 5 0.125 No Hit GGTGATAGCCGGCGCAGATTTCGGTCCTTGGAGTGAAGCATTGGAAGCGG 5 0.125 No Hit AGACAGAGGACGTGCTCTACTCCGACCGCGTCGCGATCGAGACCGCGGTG 5 0.125 No Hit TGTTGCTGGAGAAACGTGGTAATGTCATGAGGGAGAAGGGGATACTGGTT 5 0.125 No Hit GGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCG 5 0.125 No Hit GCGGCACTCAGGATCGCCAACGTCAACCCTTATAAGGCGATTTTCTTCGA 5 0.125 No Hit CGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGT 5 0.125 No Hit CGCTGCCAGAGCTGTGGGAGGAGGCCGAGAAGACAGAGGACGTGCTCTAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGATGC 60 6.5906043E-4 44.479164 2 GCGACGC 60 6.5906043E-4 44.479164 7 GATGCGA 60 6.5906043E-4 44.479164 4 TGCGACG 60 6.5906043E-4 44.479164 6 ATGCGAC 60 6.5906043E-4 44.479164 5 CGACGCA 60 6.5906043E-4 44.479164 8 CGATGCG 60 6.5906043E-4 44.479164 3 GTCGATG 65 9.776776E-4 41.057693 1 GACGCAA 65 9.776776E-4 41.057693 9 GAGGGCA 75 0.003301216 15.141844 140-144 TAGTGGA 70 0.0043871533 14.523809 110-114 GCCGATA 70 0.0043871533 14.523809 105-109 CCGATAG 70 0.0043871533 14.523809 105-109 GTCCGAG 75 0.0048917467 14.280936 135-139 AGGGCAA 80 0.0050714123 14.195479 140-144 CACTCGT 75 0.005588388 14.0 130-134 GCACTCG 75 0.005588388 14.0 130-134 CCCAGCA 75 0.006230938 13.774193 125-129 CCCCAGC 75 0.006230938 13.774193 125-129 GAAACCG 75 0.006504785 13.685897 115-119 >>END_MODULE Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra Read 1227115 spots for ERR5262799.sra Written 1227115 spots for ERR5262799.sra Read 1227100 spots for ERR5262799.sra Written 1227100 spots for ERR5262799.sra SRR ids: ['ERR5262799.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rwcxqjas ERR5262799.sra spots: 24542015 blocks: [[1, 1227100], [1227101, 2454200], [2454201, 3681300], [3681301, 4908400], [4908401, 6135500], [6135501, 7362600], [7362601, 8589700], [8589701, 9816800], [9816801, 11043900], [11043901, 12271000], [12271001, 13498100], [13498101, 14725200], [14725201, 15952300], [15952301, 17179400], [17179401, 18406500], [18406501, 19633600], [19633601, 20860700], [20860701, 22087800], [22087801, 23314900], [23314901, 24542015]] ERR5262799 file size 8033611 ERR5262799 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262799 ERR5262799_1.fastq ERR5262799_2.fastq Input file: ERR5262799_1.fastq Paired file: ERR5262799_2.fastq trimmed: ERR5262799-trimmed-pair1.fastq, ERR5262799-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:00:40 2024 >> started Fri Dec 6 12:01:03 2024 >> done (22.777s) 24542015 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 24542015 (100.00%) read pairs available; of these: 7198 ( 0.03%) trimmed read pairs available after processing 24534817 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 1 0.00% 22 1 0.00% 23 1 0.00% 24 0 0.00% 25 2 0.00% 26 3 0.00% 27 1 0.00% 28 0 0.00% 29 3 0.00% 30 0 0.00% 31 1 0.00% 32 1 0.00% 33 2 0.00% 34 2 0.00% 35 3 0.00% 36 0 0.00% 37 1 0.00% 38 2 0.00% 39 3 0.00% 40 0 0.00% 41 1 0.00% 42 0 0.00% 43 0 0.00% 44 1 0.00% 45 3 0.00% 46 1 0.00% 47 0 0.00% 48 2 0.00% 49 285 0.00% 50 295 0.00% 51 352 0.00% 52 362 0.00% 53 424 0.00% 54 492 0.00% 55 505 0.00% 56 561 0.00% 57 625 0.00% 58 691 0.00% 59 810 0.00% 60 941 0.00% 61 1173 0.00% 62 1218 0.00% 63 1461 0.01% 64 1482 0.01% 65 1708 0.01% 66 1851 0.01% 67 2135 0.01% 68 2357 0.01% 69 2676 0.01% 70 3037 0.01% 71 3425 0.01% 72 4060 0.02% 73 4675 0.02% 74 4953 0.02% 75 5634 0.02% 76 5876 0.02% 77 6330 0.03% 78 7233 0.03% 79 8058 0.03% 80 8807 0.04% 81 10082 0.04% 82 11515 0.05% 83 12572 0.05% 84 13813 0.06% 85 14865 0.06% 86 16042 0.07% 87 16788 0.07% 88 17947 0.07% 89 18750 0.08% 90 20439 0.08% 91 21978 0.09% 92 23756 0.10% 93 25951 0.11% 94 27170 0.11% 95 29061 0.12% 96 30102 0.12% 97 31614 0.13% 98 32530 0.13% 99 34089 0.14% 100 35520 0.14% 101 36802 0.15% 102 38955 0.16% 103 41561 0.17% 104 43441 0.18% 105 45236 0.18% 106 46960 0.19% 107 47933 0.20% 108 48471 0.20% 109 50684 0.21% 110 51014 0.21% 111 53943 0.22% 112 55997 0.23% 113 57406 0.23% 114 59936 0.24% 115 61714 0.25% 116 63323 0.26% 117 64396 0.26% 118 65581 0.27% 119 65745 0.27% 120 67211 0.27% 121 68534 0.28% 122 70539 0.29% 123 72254 0.29% 124 75515 0.31% 125 76607 0.31% 126 77672 0.32% 127 78703 0.32% 128 79510 0.32% 129 80786 0.33% 130 80776 0.33% 131 81528 0.33% 132 83678 0.34% 133 85002 0.35% 134 86498 0.35% 135 89132 0.36% 136 90887 0.37% 137 89655 0.37% 138 92926 0.38% 139 93420 0.38% 140 96011 0.39% 141 97224 0.40% 142 101180 0.41% 143 100667 0.41% 144 105022 0.43% 145 102972 0.42% 146 107551 0.44% 147 213854 0.87% 148 97618 0.40% 149 98357 0.40% 150 20272516 82.60% 24542015 reads passed initial QC criterion=sequence-density sequence-density=2.16 sequence-density-rank=1 fanout-score=3.91 fanout-score-rank=30 prefix-density=0.57 prefix-fanout=3.9 sequence=ACACACACACAAGCCGAGAACAGGCAGTAATAAGCCAAGCCACCGCCGCCCTTTTCTTCCATCGATCTCTCCGGCGTAAGATGAAGCTATCATTGAATTAGCCGCCGCGGCCGGCAACTGTCGAGACGAGAGAGCGCGCGCGGGAACGTCGGTAGCGGCAGACCACAACAAACCTCCTTCCATCGTCCCATCTGATCTCCCGGATACTCATGGCGCGCGCTGGACGAGAGTAGAGGTCGAGGTCCGCATGGGGGGCCCTCTCAAGCCCGGGCGTAGGTGCGGCCGAGGTGGGCGGCGCGGAGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=130.91 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=3.3 sequence=CCTTGGTCATGAAGACCTTTACACGAAGTCCAAACCGTTCTATTACAGTACATACCTATCGCTTGTGGTACTGCCTCATACTAAAACAATCACCTCCATCCACATCTATAAAAGGGGTGTTGCAAAAAAAAGGGCGGCAAAATTATGACATACATAAGATAGACCATGCAAAAGACACTAGGTGATTCGAAATAGGTGACTGACGAACATAACATCGTTTTCCTAATAGCTCATCGAACTAAGTGTCGGGCTTCGGATAGCATCAAGCTGAGAGAGAGCAGCCTCGATCATCTCCTTTGTCTGGAAGCGGTCCATATCATCCACCTCGATGAAGAGCGTGTGCATGAGGCATCCGGAGACGGAGGTGATGTTGGCGGTGATGACC criterion=sequence-density sequence-density=6.56 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=33 prefix-density=6.54 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.17 sequence-density-rank=26 fanout-score=80.63 fanout-score-rank=1 prefix-density=6.71 prefix-fanout=2.1 sequence=GTTCCGGTTCGCGGCTAGCAGTAGTTGTTGTAGTAGCAGCTAGGGTTTCCGGTAGGGTTCCGTCGAGATCGCCATGGATGAGTACCGCTGCTTCGTGGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x ACACACACACAAGCCGAGAACAGGCAGTAATAAGCCAAGCCACCGCCGCCCTTTTCTTCCATCGATCTCTCCGGCGTAAGATGAAGCTATCATTGAATTAGCCGCCGCGGCCGGCAACTGTCGAGACGAGAGAGCGCGCGCGGGAACGTCGGTAGCGGCAGACCACAACAAACCTCCTTCCATCGTCCCATCTGATCTCCCGGATACTCATGGCGCGCGCTGGACGAGAGTAGAGGTCGAGGTCCGCATGGGGGGCCCTCTCAAGCCCGGGCGTAGGTGCGGCCGAGGTGGGCGGCGCGGAGG -y CGGTTCCGGTTC -o ERR5262799 ERR5262799_1.fastq ERR5262799_2.fastq Input file: ERR5262799_1.fastq Paired file: ERR5262799_2.fastq trimmed: ERR5262799-trimmed-pair1.fastq, ERR5262799-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): ACACACACACAAGCCGAGAACAGGCAGTAATAAGCCAAGCCACCGCCGCCCTTTTCTTCCATCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:03:57 2024 >> started Fri Dec 6 12:04:13 2024 >> done (15.677s) 14725209 read pairs processed; of these: 467 ( 0.00%) short read pairs filtered out after trimming by size control 1849 ( 0.01%) empty read pairs filtered out after trimming by size control 14722893 (99.98%) read pairs available; of these: 84 ( 0.00%) trimmed read pairs available after processing 14722809 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 2 0.00% 26 2 0.00% 27 1 0.00% 28 0 0.00% 29 0 0.00% 30 0 0.00% 31 0 0.00% 32 0 0.00% 33 1 0.00% 34 2 0.00% 35 3 0.00% 36 0 0.00% 37 1 0.00% 38 1 0.00% 39 1 0.00% 40 0 0.00% 41 1 0.00% 42 0 0.00% 43 0 0.00% 44 1 0.00% 45 2 0.00% 46 0 0.00% 47 0 0.00% 48 2 0.00% 49 38 0.00% 50 220 0.00% 51 324 0.00% 52 84 0.00% 53 51 0.00% 54 69 0.00% 55 52 0.00% 56 313 0.00% 57 491 0.00% 58 635 0.00% 59 727 0.00% 60 881 0.01% 61 397 0.00% 62 134 0.00% 63 1113 0.01% 64 1402 0.01% 65 753 0.01% 66 655 0.00% 67 2003 0.01% 68 850 0.01% 69 1936 0.01% 70 1471 0.01% 71 2681 0.02% 72 1927 0.01% 73 2486 0.02% 74 2957 0.02% 75 3464 0.02% 76 3414 0.02% 77 4114 0.03% 78 4918 0.03% 79 4013 0.03% 80 6241 0.04% 81 5719 0.04% 82 7117 0.05% 83 7251 0.05% 84 8106 0.06% 85 8445 0.06% 86 9763 0.07% 87 9880 0.07% 88 10706 0.07% 89 11984 0.08% 90 11495 0.08% 91 13046 0.09% 92 14763 0.10% 93 14888 0.10% 94 16084 0.11% 95 17772 0.12% 96 17662 0.12% 97 19445 0.13% 98 19239 0.13% 99 20834 0.14% 100 20406 0.14% 101 22790 0.15% 102 23114 0.16% 103 23814 0.16% 104 26278 0.18% 105 26795 0.18% 106 29160 0.20% 107 27983 0.19% 108 29933 0.20% 109 29941 0.20% 110 31141 0.21% 111 32849 0.22% 112 32774 0.22% 113 35120 0.24% 114 34862 0.24% 115 37849 0.26% 116 37907 0.26% 117 38616 0.26% 118 39235 0.27% 119 38834 0.26% 120 40361 0.27% 121 40304 0.27% 122 42076 0.29% 123 43228 0.29% 124 45115 0.31% 125 45490 0.31% 126 46379 0.32% 127 47153 0.32% 128 47819 0.32% 129 48314 0.33% 130 48558 0.33% 131 48695 0.33% 132 49759 0.34% 133 51092 0.35% 134 51837 0.35% 135 53225 0.36% 136 54787 0.37% 137 53867 0.37% 138 56366 0.38% 139 56682 0.38% 140 57890 0.39% 141 58537 0.40% 142 60762 0.41% 143 59921 0.41% 144 62792 0.43% 145 62081 0.42% 146 64431 0.44% 147 126815 0.86% 148 58187 0.40% 149 59119 0.40% 150 12166815 82.64% criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=8.93 fanout-score-rank=28 prefix-density=0.20 prefix-fanout=8.9 sequence=ACACACACACAAGCCGAGAACAGGCAGTAATAAGCCAAGCCACCGCCGCCCTTTTCTTCCATCGATCTCTCCGGCGTAAGATGAAGCTATCATTGAATTAGCCGCCGCGGCCGGCAACTGTCGAGACGAGAGAGCGCGCGCGGGAACGTCGGTAGCGGCAGACCACAACAAACCTCCTTCCATCGTCCCATCTGATCTCCCGGATACTCATGGCGCGCGCTGGACGAGAGTAGAGGTCGAGGTCCGCATGGGGGGCCCTCTCAAGCCCGGGCGTAGGTGCGGCCGAGGTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=47 fanout-score=1117.69 fanout-score-rank=1 prefix-density=0.76 prefix-fanout=24.0 sequence=CGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTGGTG criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=39 prefix-density=0.59 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.09 sequence-density-rank=25 fanout-score=284.30 fanout-score-rank=1 prefix-density=0.99 prefix-fanout=25.4 sequence=GGCGGCGGCGGAG ERR5262799 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:05:18 Started mapping on | Dec 06 12:05:18 Finished on | Dec 06 12:07:24 Mapping speed, Million of reads per hour | 701.13 Number of input reads | 24539699 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 23782278 Uniquely mapped reads % | 96.91% Average mapped length | 290.41 Number of splices: Total | 20418312 Number of splices: Annotated (sjdb) | 18950356 Number of splices: GT/AG | 20145254 Number of splices: GC/AG | 233031 Number of splices: AT/AC | 13095 Number of splices: Non-canonical | 26932 Mismatch rate per base, % | 0.13% Deletion rate per base | 0.01% Deletion average length | 1.43 Insertion rate per base | 0.00% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 215327 % of reads mapped to multiple loci | 0.88% Number of reads mapped to too many loci | 1392 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.17% % of reads unmapped: other | 0.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 542094 542094 542094 N_multimapping 215327 215327 215327 N_noFeature 815724 23100168 1044797 N_ambiguous 518582 3366 65072 UnstrandedReadsAssigned:22447972 PositiveStrandReadsAssigned:678744 NegativeStrandReadsAssigned:22672409 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262799 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262799-trimmed-pair1.fastq ERR5262799-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,539,699 reads, 22,988,233 reads pseudoaligned [quant] estimated average fragment length: 248.869 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,158 rounds 52973 ERR5262799.ke.tsv 35125 ERR5262799.se.tsv 88098 total ==> ERR5262799.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 688.365 0 0 PNS24247 1044 796.131 143.345 10.446 PNS24249 1928 1680.13 265.812 9.17877 PNS24246 1044 796.131 143.345 10.446 PNS24248 1044 796.131 143.345 10.446 PNS24244 1471 1223.13 156.153 7.40682 PNS24243 293 105.751 0 0 KQK14069 1603 1355.13 33050.6 1414.98 KQK14071 474 247.32 65.8094 15.4377 ==> ERR5262799.se.tsv <== BRADI_1g14170v3 32975 BRADI_1g53295v3 143 BRADI_1g59795v3 378 BRADI_1g07683v3 0 BRADI_1g00485v3 15 BRADI_1g20270v3 933 BRADI_1g74790v3 2748 BRADI_1g09890v3 0 BRADI_1g77505v3 234 BRADI_1g48960v3 0 ERR5262799 completed mapping pipeline successfully