Starting /dee2/code/volunteer_pipeline.sh ERR5262800 current disk space = 1551383781376 free memory = 1605112640 ERR5262800 SRAfilesize d70565326af0a57b6b83a382bca23c9e ERR5262800.sra ERR5262800.sra file validated ERR5262800 is paired end ERR5262800 is conventional basespace ERR5262800 read1 length is 67-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262800_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 67-150 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7245 37.0 37.0 37.0 37.0 37.0 2 36.48625 37.0 37.0 37.0 37.0 37.0 3 36.636 37.0 37.0 37.0 37.0 37.0 4 36.707 37.0 37.0 37.0 37.0 37.0 5 36.6675 37.0 37.0 37.0 37.0 37.0 6 36.6445 37.0 37.0 37.0 37.0 37.0 7 36.677 37.0 37.0 37.0 37.0 37.0 8 36.694 37.0 37.0 37.0 37.0 37.0 9 36.634 37.0 37.0 37.0 37.0 37.0 10-14 36.6144 37.0 37.0 37.0 37.0 37.0 15-19 36.5476 37.0 37.0 37.0 37.0 37.0 20-24 36.4459 37.0 37.0 37.0 37.0 37.0 25-29 36.4315 37.0 37.0 37.0 37.0 37.0 30-34 36.3218 37.0 37.0 37.0 37.0 37.0 35-39 36.3113 37.0 37.0 37.0 37.0 37.0 40-44 36.2137 37.0 37.0 37.0 37.0 37.0 45-49 36.138999999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.1678 37.0 37.0 37.0 37.0 37.0 55-59 36.1177 37.0 37.0 37.0 37.0 37.0 60-64 36.108999999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.0929158539635 37.0 37.0 37.0 37.0 37.0 70-74 36.09748507662183 37.0 37.0 37.0 37.0 37.0 75-79 36.06603301650826 37.0 37.0 37.0 37.0 37.0 80-84 36.061885033084465 37.0 37.0 37.0 37.0 37.0 85-89 35.99654741055792 37.0 37.0 37.0 37.0 37.0 90-94 36.03224102799834 37.0 37.0 37.0 37.0 37.0 95-99 35.94596897400461 37.0 37.0 37.0 37.0 37.0 100-104 35.89566907551137 37.0 37.0 37.0 37.0 37.0 105-109 35.94782080043775 37.0 37.0 37.0 37.0 37.0 110-114 35.9406824701703 37.0 37.0 37.0 37.0 37.0 115-119 35.91678093649132 37.0 37.0 37.0 37.0 37.0 120-124 35.797888404339396 37.0 37.0 37.0 37.0 37.0 125-129 35.77397996710208 37.0 37.0 37.0 37.0 37.0 130-134 35.860380656071676 37.0 37.0 37.0 37.0 37.0 135-139 35.73414328901646 37.0 37.0 37.0 37.0 37.0 140-144 35.71547628353264 37.0 37.0 37.0 37.0 37.0 145-149 35.63544051210174 37.0 37.0 37.0 37.0 37.0 150 35.76491435321914 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 9.0 22 5.0 23 5.0 24 9.0 25 10.0 26 4.0 27 11.0 28 12.0 29 14.0 30 30.0 31 32.0 32 48.0 33 76.0 34 130.0 35 356.0 36 2851.0 37 395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.85 12.375 11.85 29.925 2 16.737553164873656 13.360020015011258 41.030773079809855 28.87165374030523 3 15.15 18.875 31.85 34.125 4 20.525 21.05 29.425 28.999999999999996 5 18.875 29.125 29.125 22.875 6 18.725 33.875 25.95 21.45 7 15.125 22.625 43.625 18.625 8 13.175 21.475 40.425 24.925 9 16.425 22.475 37.875 23.225 10-14 17.93 28.294999999999998 32.440000000000005 21.335 15-19 16.145 27.310000000000002 31.91 24.635 20-24 16.845 29.685 30.375000000000004 23.095 25-29 16.919999999999998 30.055 28.935 24.09 30-34 15.53 32.45 27.725 24.295 35-39 16.055 30.175 29.110000000000003 24.66 40-44 17.59 30.34 27.500000000000004 24.57 45-49 15.015 33.040000000000006 26.729999999999997 25.215 50-54 16.23 33.495000000000005 25.515 24.759999999999998 55-59 19.759999999999998 32.755 24.825 22.66 60-64 20.455000000000002 31.374999999999996 25.64 22.53 65-69 22.517251725172517 29.892989298929894 25.992599259925992 21.597159715971596 70-74 19.86195168308908 32.03121092382334 26.394237983294154 21.71259940979343 75-79 19.994997498749374 31.735867933966983 24.542271135567784 23.72686343171586 80-84 20.05803772452094 34.10716966027918 24.961224796117477 20.873567819082403 85-89 19.824868651488618 30.522892169126848 27.420565424068048 22.231673755316486 90-94 19.736314417485463 30.945458191297377 23.37577702025266 25.942450370964508 95-99 22.28536941105467 30.795151637076902 25.619876276215862 21.29960267565257 100-104 21.56238953693885 29.843963035903652 27.409988385598144 21.18365904155936 105-109 22.205852469010363 28.0735622840886 25.807762649867914 23.912822597033124 110-114 23.8329490135793 26.93825262618499 29.008455034588778 20.22034332564694 115-119 21.956521739130437 27.836438923395445 29.61697722567288 20.59006211180124 120-124 19.52268554943614 29.934434828219246 27.883556254917387 22.659323367427223 125-129 20.18065887353879 30.58448459086079 27.337938363443147 21.896918172157278 130-134 23.037330010294195 29.57143631142656 25.556699355258168 21.834534323021078 135-139 20.253653156878965 29.214226633581475 24.72015439757375 25.811965811965816 140-144 19.536535859269282 28.382949932341 27.734551195308978 24.34596301308074 145-149 20.74018301864937 26.03961542916715 27.748175605235726 25.472025946947756 150 21.204961606615473 23.331364441819254 23.301831069108093 32.16184288245717 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.0 11 1.5 12 1.0 13 2.0 14 3.0 15 2.0 16 0.5 17 0.0 18 0.5 19 1.5 20 1.5 21 1.0 22 3.0 23 5.0 24 4.0 25 2.5 26 5.0 27 14.0 28 22.0 29 20.0 30 29.0 31 69.0 32 103.5 33 139.0 34 135.5 35 107.0 36 143.0 37 205.5 38 199.0 39 164.0 40 178.5 41 171.0 42 219.0 43 233.0 44 191.0 45 203.5 46 179.0 47 156.5 48 143.0 49 97.0 50 121.5 51 181.0 52 194.5 53 127.5 54 74.5 55 80.0 56 37.0 57 6.0 58 1.0 59 2.5 60 4.0 61 4.0 62 1.5 63 2.0 64 2.5 65 0.5 66 0.0 67 0.5 68 0.5 69 0.0 70 1.0 71 1.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 0.0 70-71 0.0 72-73 1.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.0 90-91 2.0 92-93 12.0 94-95 3.0 96-97 4.0 98-99 2.0 100-101 17.0 102-103 4.0 104-105 12.0 106-107 7.0 108-109 15.0 110-111 16.0 112-113 9.0 114-115 15.0 116-117 25.0 118-119 19.0 120-121 18.0 122-123 24.0 124-125 8.0 126-127 35.0 128-129 26.0 130-131 36.0 132-133 19.0 134-135 21.0 136-137 31.0 138-139 46.0 140-141 19.0 142-143 34.0 144-145 44.0 146-147 39.0 148-149 47.0 150-151 3386.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.049999999999997 #Duplication Level Percentage of deduplicated Percentage of total 1 42.68502581755594 12.4 2 18.588640275387263 10.8 3 12.048192771084338 10.5 4 7.74526678141136 9.0 5 4.905335628227195 7.124999999999999 6 3.356282271944923 5.8500000000000005 7 2.495697074010327 5.075 8 1.2048192771084338 2.8000000000000003 9 1.376936316695353 3.5999999999999996 >10 5.335628227194492 27.725 >50 0.25817555938037867 5.125 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCAAACTCATCGACCGGTGTCACCTTTCAACGATCCTGCACTGACCAAT 79 1.975 No Hit GTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATC 65 1.625 No Hit GCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACC 61 1.525 No Hit GGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACC 40 1.0 No Hit TTTTTTTTTTGTCTGAAACTCCATTTATTTTATTTAACATATCCAGATTA 39 0.975 No Hit GTCCTACACTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACC 38 0.95 No Hit GTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACA 38 0.95 No Hit GCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCA 36 0.8999999999999999 No Hit GCCCTGGCTTTTTACCATTTAAGACAGCACTCTCTACTGGCTCCACACCA 33 0.8250000000000001 No Hit GTGGCCCTGGCTTTTTACCATTTAAGACAGCACTCTCTACTGGCTCCACA 31 0.775 No Hit CACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCA 29 0.7250000000000001 No Hit GGAAGAATTGGAGGATTAATTTGGTGCTGTGTTCTTTGTTTTTTTTTTGT 28 0.7000000000000001 No Hit GTTCCATTAACAACTGGAATGTGCAGTTTGAAGGCTCAATAACCATAACA 27 0.675 No Hit CTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAG 26 0.65 No Hit TTTTTTTTTTTGTCTGAAACTCCATTTATTTTATTTAACATATCCAGATT 25 0.625 No Hit CTTGGATCTTGTGTGGCCCTGGCTTTTTACCATTTAAGACAGCACTCTCT 25 0.625 No Hit GTCAAGGTAAATGGCGCCGCTGGATAGGTACTCGATTTCTAGTTTTCTAC 24 0.6 No Hit GTTCACAGTAAGGACAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCA 22 0.5499999999999999 No Hit GCCGCCGCGGCCATTTCTTGGACCAGCATTAAAGCACACAAAAAAAGCTC 22 0.5499999999999999 No Hit GCGCCGCTGGATAGGTACTCGATTTCTAGTTTTCTACTCTCGGATGACAG 22 0.5499999999999999 No Hit CTACACTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACCAAA 21 0.525 No Hit CCTACACTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACCAA 20 0.5 No Hit CTTTGAATGACAGCTACTTGTTGCATTACCAAAATCTCTGTTATGAGAAC 20 0.5 No Hit GTTCTTTGTTTTTTTTTTGTTTTCCTGAAGCTAAGCGAGATTAGAGAGAA 19 0.475 No Hit GACAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCT 19 0.475 No Hit GGACAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTC 19 0.475 No Hit GCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTT 18 0.44999999999999996 No Hit GGCGCCGCTGGATAGGTACTCGATTTCTAGTTTTCTACTCTCGGATGACA 18 0.44999999999999996 No Hit GTTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCG 16 0.4 No Hit CACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAGTGA 16 0.4 No Hit CTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACC 16 0.4 No Hit GCTACTTGTTGCATTACCAAAATCTCTGTTATGAGAACCTTCTGTCAAGG 16 0.4 No Hit GGCCCTGGCTTTTTACCATTTAAGACAGCACTCTCTACTGGCTCCACACC 15 0.375 No Hit CTCACCTTGTCTTTTAATCGAAACTTACAAATAACCAAAAAAGACATCTA 15 0.375 No Hit CTAGCATCCATGCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCTC 15 0.375 No Hit CTAGATTTTTTTCCAAACTATTAGGAACAGCAGAACCAACCTGCGGCACA 14 0.35000000000000003 No Hit GGATCTTGTGTGGCCCTGGCTTTTTACCATTTAAGACAGCACTCTCTACT 14 0.35000000000000003 No Hit GCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAGTG 14 0.35000000000000003 No Hit CACTTATTTACATGACATCCAAAGTTCCTTTTTCTCAGATTTTGAGCATC 14 0.35000000000000003 No Hit GTTGCATTACCAAAATCTCTGTTATGAGAACCTTCTGTCAAGGTAAATGG 14 0.35000000000000003 No Hit TTTTTTTTTGTCTGAAACTCCATTTATTTTATTTAACATATCCAGATTAA 13 0.325 No Hit AGCTACTTGTTGCATTACCAAAATCTCTGTTATGAGAACCTTCTGTCAAG 13 0.325 No Hit CTTGTCTTTTAATCGAAACTTACAAATAACCAAAAAAGACATCTAGATTT 13 0.325 No Hit CGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCAC 13 0.325 No Hit GCACAACACTGCATTCCTCCAAGCTTTCAAATGGATGCAACTACTATGAT 13 0.325 No Hit CTGTCATTTATACATTTTCTGTAGGCAAAAAAATTGGTAAAAAAAATATC 12 0.3 No Hit ACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCGACT 12 0.3 No Hit GCCGCGGCCATTTCTTGGACCAGCATTAAAGCACACAAAAAAAGCTCACA 11 0.27499999999999997 No Hit GGGAAAAGAAAAGAATGGAGCATTGTCAATACGGTGCTCTTTGTTTATTG 11 0.27499999999999997 No Hit GGAATTTCAACTAAAACTATCTATATGTACCTATTTTATTTACAAGGGCA 10 0.25 No Hit TTTTTTTTTTTTGTCTGAAACTCCATTTATTTTATTTAACATATCCAGAT 10 0.25 No Hit GGAGTAGTGGAAGAATTGGAGGATTAATTTGGTGCTGTGTTCTTTGTTTT 10 0.25 No Hit GCCTTATTTCTTTGGTTTAGAGGGCAGGCAGACTGCACCCTGAGACCCCT 10 0.25 No Hit CTCCAAACCAAGAGTAAAAAAAAAAAAACGATACCAGCAACTGAACTTAA 10 0.25 No Hit CATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGTTTTGATTC 10 0.25 No Hit GCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTTCAACAGAAT 10 0.25 No Hit AGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCAC 10 0.25 No Hit ATGACATCCAAAGTTCCTTTTTCTCAGATTTTGAGCATCTGTTTGATTGC 10 0.25 No Hit GTCAACCACCACCAAAGCACCATCAGTGATACGAAGGGCGGCTGTGACCT 10 0.25 No Hit CCTCAATACAGTCAACCACCACCAAAGCACCATCAGTGATACGAAGGGCG 10 0.25 No Hit GTGCTGACGCCTCGATCCTTCAGGGCAGAAGAGTGCCATGAGAGGAAATT 10 0.25 No Hit AGATTTTTTTCCAAACTATTAGGAACAGCAGAACCAACCTGCGGCACAAA 10 0.25 No Hit GGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCAC 10 0.25 No Hit ATCCAAAGTTCCTTTTTCTCAGATTTTGAGCATCTGTTTGATTGCAATTT 10 0.25 No Hit GTCACATATATCAATTGATTTTGCAAATTGCTTGAGTTCGGTCCATGGAT 10 0.25 No Hit ACGCCGCCGCGGCCATTTCTTGGACCAGCATTAAAGCACACAAAAAAAGC 9 0.22499999999999998 No Hit CCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACAC 9 0.22499999999999998 No Hit GTATGGAGTATGCTTGTGCACAACAGGATCTTTGTCACATATATCAATTG 9 0.22499999999999998 No Hit TGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAAC 9 0.22499999999999998 No Hit CAGTAAGGACAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTT 9 0.22499999999999998 No Hit CCCACTACAGATTTTATTACAGTTGCGCTGTCAAATTTTAACCCTTTTGT 9 0.22499999999999998 No Hit GTCGCTGCGAAGATTTTTTTTTTTGGCCGATACTTACTCTCATCTTGAGA 9 0.22499999999999998 No Hit CTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCT 9 0.22499999999999998 No Hit GTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGTTTTGA 9 0.22499999999999998 No Hit GTTATGAGAACCTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTACTCG 9 0.22499999999999998 No Hit CCTGTCTCATCTGCAGCAAAGATCATAGGATTGCAGTCAAATCCAACACC 9 0.22499999999999998 No Hit CCCACTCTTTGATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCGTG 9 0.22499999999999998 No Hit GTGCAAAGTCTTTCTTCTTGTATACCACCACCACCCAGGCACCCACTACA 9 0.22499999999999998 No Hit CCACAGAGAACATTGCGTGATAAAGATAGCTTTTTGGTAGCACCATTGTA 9 0.22499999999999998 No Hit CTCTGTTATGAGAACCTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTA 9 0.22499999999999998 No Hit CTTGCACTTTCCACAGAGAACATTGCGTGATAAAGATAGCTTTTTGGTAG 9 0.22499999999999998 No Hit AGTAAGGACAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTT 8 0.2 No Hit GTGGTCCTGGCTTTTTACCATTTAAGACAGCACTCTCTACTGGCTCCACA 8 0.2 No Hit CATGCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATT 8 0.2 No Hit ATATGTTCCATTAACAACTGGAATGTGCAGTTTGAAGGCTCAATAACCAT 8 0.2 No Hit GCAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTG 8 0.2 No Hit GAGTAGTGGAAGAATTGGAGGATTAATTTGGTGCTGTGTTCTTTGTTTTT 8 0.2 No Hit ATCCTCACTCCCGCACCACGACAACCATGACAAGTAGCCGTTGCCCCACT 8 0.2 No Hit GCGACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAATAAC 8 0.2 No Hit GCTATACATAGGTTAGTACCACTGGAATTTCAACTAAAACTATCTATATG 8 0.2 No Hit GCCGTTGGATAGGTACTCGATTTCTAGTTTTCTACTCTCGGATGACAGCA 8 0.2 No Hit GCCTCATTTGGACTTGTATTTTCATCACCCGGTTTTGTGGATGAAGTATA 8 0.2 No Hit CTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACCAAAATCTC 8 0.2 No Hit CCATCATTGTCCTCCAAACCAAGAGTAAAAAAAAAAAAAACGATACCAGC 8 0.2 No Hit GCAGGATCGAACCTCCTGTATTAACTCTTAAACATCTGACATCTCTATCT 8 0.2 No Hit CTCAAACATCTAAAGTAATTACACTACCGCGAAATTCACGGCTATTTAGA 7 0.17500000000000002 No Hit CACCACCATTGTAAGATGTTATCTCATTGATATGTTCCATTAACAACTGG 7 0.17500000000000002 No Hit CCTCTTCCTATTTTTTTCAACAGAATATCTGAACACTTTTATGAGCTCTG 7 0.17500000000000002 No Hit TATGGCGCCGCTGGATAGGTACTCGATTTCTAGTTTTCTACTCTCGGATG 7 0.17500000000000002 No Hit GAAGAATTGGAGGATTAATTTGGTGCTGTGTTCTTTGTTTTTTTTTTGTT 7 0.17500000000000002 No Hit CTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCCTCAGCTTCCTTAAG 7 0.17500000000000002 No Hit TTTTTTTTTTGTTTTCCTGAAGCTAAGCGAGATTAGAGAGAAACGTTGCA 7 0.17500000000000002 No Hit AGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCA 7 0.17500000000000002 No Hit GCTGTGTTCTTTGTTTTTTTTTTGTTTTCCTGAAGCTAAGCGAGATTAGA 7 0.17500000000000002 No Hit CATCTAGATTTTTTTCCAAACTATTAGGAACAGCAGAACCAACCTGCGGC 7 0.17500000000000002 No Hit CTTTGTTTATTGTCTGTCATTTATACATTTTCTGTAGGCAAAAAAATTGG 7 0.17500000000000002 No Hit GGAGGATTAATTTGGTGCTGTGTTCTTTGTTTTTTTTTTGTTTTCCTGAA 7 0.17500000000000002 No Hit GTGTGGCCCTGGCTTTTTACCATTTAAGACAGCACTCTCTACTGGCTCCA 7 0.17500000000000002 No Hit GCTGACGCCTCGATCCTTCAGGGCAGAAGAGTGCCATGAGAGGAAATTGG 7 0.17500000000000002 No Hit CTTCGTTTTGTTTCCATCATTGTCCTCCAAACCAAGAGTAAAAAAAAAAA 7 0.17500000000000002 No Hit TTTTTTTGTCTGAAACTCCATTTATTTTATTTAACATATCCAGATTAACA 7 0.17500000000000002 No Hit CATGAGAAGGTTGCAACTCTTCATAAAGAACAGCTTATCACCACATATGG 7 0.17500000000000002 No Hit GTGGAAGAATTGGAGGATTAATTTGGTGCTGTGTTCTTTGTTTTTTTTTT 7 0.17500000000000002 No Hit AGCATTGGCAATGGTGATGGCACCAGGGTTCTGGCTGCTAAGTGCAGCAA 7 0.17500000000000002 No Hit GCTCTTTGTTTATTGTCTGTCATTTATACATTTTCTGTAGGCAAAAAAAT 7 0.17500000000000002 No Hit ATGCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATTT 7 0.17500000000000002 No Hit GGGCAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTC 7 0.17500000000000002 No Hit CTTGACAGATGCAAAATATATCAATTAAATGTGTAGCCTAGCAAAATGTC 7 0.17500000000000002 No Hit ACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAGTGAT 7 0.17500000000000002 No Hit CTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTTC 7 0.17500000000000002 No Hit CAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATA 7 0.17500000000000002 No Hit ATTTTTTTCCAAACTATTAGGAACAGCAGAACCAACCTGCGGCACAAACC 7 0.17500000000000002 No Hit CGCCGCCGCGGCCATTTCTTGGACCAGCATTAAAGCACACAAAAAAAGCT 7 0.17500000000000002 No Hit CCACTACAGATTTTATTACAGTTGCGCTGTCAAATTTTAACCCTTTTGTT 7 0.17500000000000002 No Hit AGCGACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAATAA 6 0.15 No Hit ATCGACAGATCGCCGCCGCGGCCATTTCTTGGACCAGCATTAAAGCACAC 6 0.15 No Hit CATGAAACGTGTTCCTCAACACCAATGACTGAGCCTCTACGGGCTACGGA 6 0.15 No Hit GTCTTTTAATCGAAACTTACAAATAACCAAAAAAGACATCTAGATTTTTT 6 0.15 No Hit ACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAATAACCAA 6 0.15 No Hit GGGGAAAAGAAAAGAATGGAGCATTGTCAATACGGTGCTCTTTGTTTATT 6 0.15 No Hit GTCAATACGGTGCTCTTTGTTTATTGTCTGTCATTTATACATTTTCTGTA 6 0.15 No Hit AAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATAC 6 0.15 No Hit GCAGATACTCTAGTTACACGGCAAAAAAGGGGAAAAGAAAAGAATGGAGC 6 0.15 No Hit GTAGTGGAAGAATTGGAGGATTAATTTGGTGCTGTGTTCTTTGTTTTTTT 6 0.15 No Hit CCTTGGAAGAACTGTTGGTGGTGTACTAAATCTGGCTGGTATCATCATGA 6 0.15 No Hit CCCCACTCTTTGATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCGT 6 0.15 No Hit CAGCGACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAATA 6 0.15 No Hit GGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACC 6 0.15 No Hit GGACAAAATACAAGTACACTTATTTACATGACATCCAAAGTTCCTTTTTC 6 0.15 No Hit TGCCAAACTCATCGACCGGTGTCACCTTTCAACGATCCTGCACTGACCAA 6 0.15 No Hit CACAAGACAGTTTTTTACACAAATTTTCATCAGCAATTTAAATTTGCACC 6 0.15 No Hit ACCACCTCAAACATCTAAAGTAATTACACTACCGCGAAATTCACGGCTAT 6 0.15 No Hit TTTTAATCGAAACTTACAAATAACCAAAAAAGACATCTAGATTTTTTTCC 6 0.15 No Hit ATTTAAGACAGCACTCTCTACTGGCTCCACACCATAAAGCTTGATATTAG 6 0.15 No Hit CTGTTATGAGAACCTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTACT 6 0.15 No Hit CCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGACAG 6 0.15 No Hit CAACTACTATGATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCCT 6 0.15 No Hit ATAGCTAGCATCCATGCCCTCTAGAAGCCTGATACAAACATCTGAACATT 6 0.15 No Hit CTGTGTTCTTTGTTTTTTTTTTGTTTTCCTGAAGCTAAGCGAGATTAGAG 6 0.15 No Hit GGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCGACTAACTG 6 0.15 No Hit GTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGC 6 0.15 No Hit CCGCACCACGACAACCATGACAAGTAGCCGTTGCCCCACTCTTTGATCCT 6 0.15 No Hit GCTTTGAATGACAGCTACTTGTTGCATTACCAAAATCTCTGTTATGAGAA 6 0.15 No Hit GGCGTCGCTGGATAGGTACTCGATTTCTAGTTTTCTACTCTCGGATGACA 6 0.15 No Hit GTTTATGAACTCATCAATCTCCCTTTGTACCGCGATTTTTGACTTCTTGA 6 0.15 No Hit CTCAGATTTTGAGCATCTGTTTGATTGCAATTTTTGCAACAAACATTGTA 6 0.15 No Hit AGGAAGTAAACATAAACTGCCAAACTCATCGACCGGTGTCACCTTTCAAC 6 0.15 No Hit GTCTGTCATTTATACATTTTCTGTAGGCAAAAAAATTGGTAAAAAAAATA 6 0.15 No Hit GACTTTTTCTCCAGATGAAGCAGCATTACTCAGAGCAGATGCAATTAAAG 6 0.15 No Hit CCTCATTTGGACTTGTATTTTCATCACCCGGTTTTGTGGATGAAGTATAC 6 0.15 No Hit CTGCCAAACTCATCGACCGGTGTCACCTTTCAACGATCCTGCACTGACCA 6 0.15 No Hit CCTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTT 6 0.15 No Hit CTCTAGTTACACGGCAAAAAAGGGGAAAAGAAAAGAATGGAGCATTGTCA 6 0.15 No Hit CTTTGGTTTAGAGGGCAGGCAGACTGCACCCTGAGACCCCTCGGCGTCGG 5 0.125 No Hit GCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTC 5 0.125 No Hit CTCCAAAGTCCTGTCTCATCTGCAGCAAAGATCATAGGATTGCAGTCAAA 5 0.125 No Hit CTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTACTCGATTTCTAGTTT 5 0.125 No Hit TTGCACTTTCCACAGAGAACATTGCGTGATAAAGATAGCTTTTTGGTAGC 5 0.125 No Hit ATTTCTTTGGTTTAGAGGGCAGGCAGACTGCACCCTGAGACCCCTCGGCG 5 0.125 No Hit ATTAATTTGGTGCTGTGTTCTTTGTTTTTTTTTTGTTTTCCTGAAGCTAA 5 0.125 No Hit CCTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTTCAACAGAATA 5 0.125 No Hit CCTTGTCTTTTAATCGAAACTTACAAATAACCAAAAAAGACATCTAGATT 5 0.125 No Hit TTCCTATTTTTTTCAACAGAATATCTGAACACTTTTATGAGCTCTGGGAG 5 0.125 No Hit CTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCA 5 0.125 No Hit GTTAGTACCACTGGAATTTCAACTAAAACTATCTATATGTACCTATTTTA 5 0.125 No Hit GTTGCGCTGTCAAATTTTAACCCTTTTGTTGCCGATTCTCTCAAGGGGAA 5 0.125 No Hit GCCCATTCCCCTTACTCTTATATGCATCATGACTGAGTGATAAGAAAAAT 5 0.125 No Hit GTTTATTGTCTGTCATTTATACATTTTCTGTAGGCAAAAAAATTGGTAAA 5 0.125 No Hit TCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCA 5 0.125 No Hit GCTCGACCCAAAAATTATTGAGGAAGGAAGTAAACATAAACTGCCAAACT 5 0.125 No Hit GGCTATTTAGATAGCTAGCATCCATGCCCTCTAGAAGCCTGATACAAACA 5 0.125 No Hit ATTTTTTTCAACAGAATATCTGAACACTTTTATGAGCTCTGGGAGACATG 5 0.125 No Hit GGTCTATTGGATTGAATATAAGATGACACAAGGCACAAGGAAGTGTCTTT 5 0.125 No Hit GTGCTCTTTGTTTATTGTCTGTCATTTATACATTTTCTGTAGGCAAAAAA 5 0.125 No Hit GACAGCTACTTGTTGCATTACCAAAATCTCTGTTATGAGAACCTTCTGTC 5 0.125 No Hit CAAGGGGAAAACTTCCTCTGCGAAAAATAAATGTGCAAAGTCTTTCTTCT 5 0.125 No Hit GTCAAATTTTAACCCTTTTGTTGCCGATTCTCTCAAGGGGAAAACTTCCT 5 0.125 No Hit CTCTTTTTTCCAATGCAAAACTCTGGATCGATAACAACTCGTACGAGTTG 5 0.125 No Hit GCCCCACTCTTTGATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCG 5 0.125 No Hit GATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCGTGATAAAGATAG 5 0.125 No Hit TCTGCATCAGCTAAAGGGGCTTCCTCACTTTCCTTGTTCTTCTTTTTCTT 5 0.125 No Hit GCTCTATGGTCTATTTCTGTCTCTTGGTGGATGCAAAAACCCTCATTTGC 5 0.125 No Hit GTGTTCTTTGTTTTTTTTTTGTTTTCCTGAAGCTAAGCGAGATTAGAGAG 5 0.125 No Hit GCCACATTTATCATCATTTGCTCCAGGTATTAAATCCCGATGAGACCCCA 5 0.125 No Hit GGGGCCGAGGGCATTTACAGCTTTTACAGGGTCGAAACCCACTGAAGAAT 5 0.125 No Hit GATTAATTTGGTGCTGTGTTCTTTGTTTTTTTTTTGTTTTCCTGAAGCTA 5 0.125 No Hit GACTTTTCTAAAGCTACAGGAAAAAATAAAATACAACCACAACTTGCCAA 5 0.125 No Hit CAACAATTTAGGATCGGAGTGAGTACATATAGAAAGCCTTATTTCTTTGG 5 0.125 No Hit CCGGTTTTGTGGATGAAGTATACAGCTTACTATTAAACCTTCCTCTTTGA 5 0.125 No Hit GCTTTGAATCATAATTAGCAAAAGCAAATAGGTTCAATGGTGTGAATTGA 5 0.125 No Hit GCCCATTTTTCTGCAAGCCTCACAAGGATAACAATGTATGGAGTATGCTT 5 0.125 No Hit GCCTTGGCCATTTCAGTGTAATCCTGTGGAACCAAATATGATTGTCAAAC 5 0.125 No Hit TTCACATCCCTGTGGCAAACTTCAGGCACAGTATGAATGTAGGCTAAACC 5 0.125 No Hit GGGACAGGTCTAATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGT 5 0.125 No Hit ACAACATGGTTAAAGCACACACGGTGTAAGAAAAGTAAGGCGTAAATGTA 5 0.125 No Hit CTCATTTGGACTTGTATTTTCATCACCCGGTTTTGTGGATGAAGTATACA 5 0.125 No Hit CCAGCCCATTCCCCTTACTCTTATATGCATCATGACTGAGTGATAAGAAA 5 0.125 No Hit ATATCCTCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAG 5 0.125 No Hit CTATGATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCCTCAGCTT 5 0.125 No Hit ATCTAATTACTACTGAAAAGTAAAATGTAGAATATACACAAAATCAGCCC 5 0.125 No Hit CACTCTTTGATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCGTGAT 5 0.125 No Hit ATGTCCTACACTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTA 5 0.125 No Hit TCTCACCAAGAGCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCC 5 0.125 No Hit CTCATTGATATGTTCCATTAACAACTGGAATGTGCAGTTTGAAGGCTCAA 5 0.125 No Hit GGGAAGGAAGTAAACATAAACTGCCAAACTCATCGACCGGTGTCACCTTT 5 0.125 No Hit GGTTTATCTCATGAAACGTGTTCCTCAACACCAATGACTGAGCCTCTACG 5 0.125 No Hit GGTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGTTTTG 5 0.125 No Hit GCAACTACTATGATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCC 5 0.125 No Hit GCATTACCAAAATCTCTGTTATGAGAACCTTCTGTCAAGGTAAATGGCGC 5 0.125 No Hit AACGGATTTCCAAAAGAATCGGGCAAGAACCAAAAAAAAACTAAACTCGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTT 10 0.007233308 142.25 3 >>END_MODULE ERR5262800 read2 length is 59-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262800_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 59-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0035 37.0 37.0 37.0 37.0 37.0 2 35.9225 37.0 37.0 37.0 37.0 37.0 3 35.927 37.0 37.0 37.0 37.0 37.0 4 36.185 37.0 37.0 37.0 37.0 37.0 5 36.162 37.0 37.0 37.0 37.0 37.0 6 36.1645 37.0 37.0 37.0 37.0 37.0 7 36.109 37.0 37.0 37.0 37.0 37.0 8 36.1125 37.0 37.0 37.0 37.0 37.0 9 36.0835 37.0 37.0 37.0 37.0 37.0 10-14 36.222500000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.1377 37.0 37.0 37.0 37.0 37.0 20-24 36.0717 37.0 37.0 37.0 37.0 37.0 25-29 36.09009999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.011100000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.030800000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.0141 37.0 37.0 37.0 37.0 37.0 45-49 35.9533 37.0 37.0 37.0 37.0 37.0 50-54 35.905100000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.8589 37.0 37.0 37.0 37.0 37.0 60-64 35.899124781195304 37.0 37.0 37.0 37.0 37.0 65-69 35.918229557389346 37.0 37.0 37.0 37.0 37.0 70-74 35.8463004195271 37.0 37.0 37.0 37.0 37.0 75-79 35.79959979989995 37.0 37.0 37.0 37.0 37.0 80-84 35.80171614453711 37.0 37.0 37.0 37.0 37.0 85-89 35.785689266950214 37.0 37.0 37.0 37.0 37.0 90-94 35.693777225101584 37.0 37.0 37.0 37.0 37.0 95-99 35.66084709717641 37.0 37.0 37.0 37.0 37.0 100-104 35.67492452033271 37.0 37.0 37.0 37.0 37.0 105-109 35.546732012907825 37.0 37.0 37.0 37.0 37.0 110-114 35.544120397350625 37.0 37.0 37.0 37.0 37.0 115-119 35.57218144708504 37.0 37.0 37.0 37.0 37.0 120-124 35.46327238061249 37.0 37.0 37.0 37.0 37.0 125-129 35.45769689416984 37.0 37.0 37.0 37.0 37.0 130-134 35.49026812874978 37.0 37.0 37.0 37.0 37.0 135-139 35.397229926886794 37.0 37.0 37.0 34.6 37.0 140-144 35.449893123688156 37.0 37.0 37.0 37.0 37.0 145-149 35.363846240033794 37.0 37.0 37.0 32.2 37.0 150 35.385004462957454 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 5.0 13 5.0 14 3.0 15 3.0 16 0.0 17 0.0 18 2.0 19 0.0 20 1.0 21 6.0 22 8.0 23 14.0 24 3.0 25 15.0 26 10.0 27 9.0 28 8.0 29 19.0 30 27.0 31 28.0 32 45.0 33 89.0 34 214.0 35 548.0 36 2691.0 37 243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0 24.65 8.725 22.625 2 29.225 24.175 25.974999999999998 20.625 3 24.025 26.1 30.0 19.875 4 25.85 32.65 20.875 20.625 5 25.424999999999997 37.15 19.6 17.825 6 26.025 35.55 20.3 18.125 7 24.3 24.55 30.025000000000002 21.125 8 23.75 26.55 23.925 25.775 9 24.675 26.125 26.375 22.825 10-14 26.424999999999997 28.315 23.405 21.855 15-19 24.44 28.139999999999997 23.9 23.52 20-24 25.935000000000002 27.315 24.81 21.94 25-29 24.79 27.500000000000004 24.44 23.27 30-34 26.11 29.75 24.3 19.84 35-39 23.990000000000002 29.409999999999997 25.665 20.935000000000002 40-44 24.34 28.499999999999996 25.95 21.21 45-49 23.285 27.36 27.589999999999996 21.765 50-54 23.935000000000002 26.1 28.804999999999996 21.16 55-59 23.5 27.33 26.93 22.24 60-64 23.85596399099775 26.356589147286826 28.43710927731933 21.350337584396097 65-69 23.59089772443111 25.461365341335334 28.28207051762941 22.665666416604154 70-74 24.24348521982694 25.018756564797677 28.114840194067924 22.62291802130746 75-79 22.756378189094548 26.333166583291646 28.14407203601801 22.7663831915958 80-84 23.475258918296895 25.91184269775354 28.7887126632311 21.824185720718468 85-89 24.148111083312486 25.449086815111333 27.495621716287218 22.907180385288967 90-94 24.744335271706436 26.243232404251053 27.015239623019855 21.99719270102266 95-99 23.532666096665494 28.225116934064275 27.90323391842277 20.33898305084746 100-104 24.173105085088118 27.25344644750795 28.359339494016055 20.21410897338787 105-109 23.658809185124973 27.9363950416582 28.530786425523267 19.87400934769356 110-114 24.34025108890597 27.250832692800408 28.844478606200358 19.56443761209326 115-119 25.300207039337476 26.82194616977226 27.386128364389233 20.491718426501034 120-124 24.788880146865985 28.444794125360605 27.159716758457908 19.606608969315502 125-129 24.52699829931973 26.56781462585034 29.257015306122447 19.648171768707485 130-134 25.117943712380026 26.652567648175264 29.10362778591183 19.12586085353289 135-139 23.984526112185687 25.824813484387953 29.113014644929542 21.07764575849682 140-144 23.611347035841685 28.25434573353717 28.384576184813998 19.749731045807145 145-149 24.458962841976316 27.276439363005306 28.61809484920959 19.646502945808784 150 22.404046414757513 28.830705147277598 30.258851532282062 18.50639690568283 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.5 19 1.0 20 0.0 21 0.0 22 1.5 23 4.0 24 3.0 25 0.5 26 1.0 27 2.0 28 4.5 29 6.0 30 6.0 31 13.5 32 20.0 33 23.0 34 28.0 35 34.5 36 51.0 37 81.5 38 109.0 39 118.0 40 133.0 41 143.0 42 158.5 43 198.0 44 244.5 45 300.0 46 332.0 47 412.0 48 390.5 49 240.5 50 143.5 51 83.0 52 73.5 53 75.0 54 67.0 55 59.0 56 62.0 57 49.0 58 33.5 59 39.0 60 29.5 61 17.5 62 13.0 63 21.0 64 35.0 65 26.0 66 11.0 67 12.0 68 18.5 69 19.0 70 13.0 71 8.5 72 7.5 73 5.0 74 5.5 75 5.0 76 3.0 77 2.5 78 0.0 79 0.5 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 58-59 1.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.0 90-91 2.0 92-93 12.0 94-95 3.0 96-97 4.0 98-99 2.0 100-101 17.0 102-103 4.0 104-105 12.0 106-107 7.0 108-109 15.0 110-111 16.0 112-113 9.0 114-115 15.0 116-117 25.0 118-119 19.0 120-121 18.0 122-123 24.0 124-125 8.0 126-127 36.0 128-129 28.0 130-131 36.0 132-133 20.0 134-135 24.0 136-137 33.0 138-139 48.0 140-141 24.0 142-143 36.0 144-145 51.0 146-147 39.0 148-149 47.0 150-151 3361.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.225 #Duplication Level Percentage of deduplicated Percentage of total 1 48.66785079928952 20.549999999999997 2 20.781527531083483 17.549999999999997 3 12.61101243339254 15.975 4 8.052101835405566 13.600000000000001 5 3.256364712847839 6.875000000000001 6 2.1314387211367674 5.4 7 1.4209591474245116 4.2 8 0.6512729425695678 2.1999999999999997 9 0.5920663114268798 2.25 >10 1.8354055654233272 11.4 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTTGTTACTCACA 26 0.65 No Hit CCGAAACAAGGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTTGT 26 0.65 No Hit GTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAG 24 0.6 No Hit CCTCAAGAGCCACCGCCGCACGCGAACCGAGGATCAGTCGCTCGCGCTTC 22 0.5499999999999999 No Hit GGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTT 21 0.525 No Hit ACCGAAACAAGGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTTG 19 0.475 No Hit GGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAA 17 0.42500000000000004 No Hit GGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATT 16 0.4 No Hit AATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGATTTTTC 16 0.4 No Hit GGGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTG 15 0.375 No Hit CTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAG 15 0.375 No Hit GGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGA 14 0.35000000000000003 No Hit GTCCAAAAGGCGTTTGTGAGGAAGCCTGGAAACTAGAAGATGTCCGATTT 14 0.35000000000000003 No Hit CACACCGAAACAAGGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCG 13 0.325 No Hit AGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACCTGATC 13 0.325 No Hit GAAGCATTGACTGTATCGTTACTGTGTCCTATCCATGCTGTCATCCGAGA 13 0.325 No Hit GTGGTAGTTCAAGGGGACGCAGACAGAAGCGTGGTGAAGATGTGGTGCAC 13 0.325 No Hit CTGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATAC 13 0.325 No Hit GGACAAGAAGAACAACATCCGCAACATGTCTGTTATTGCTCATGTGGACC 12 0.3 No Hit GTTCGCATGACTGATACCCGTGCAGATGAAGCAGAGCGTGGTATTACGAT 12 0.3 No Hit CACCGAAACAAGGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTT 12 0.3 No Hit GTTCATCCATCCACTAGTAGCTTCCCTGTGCTTCTTGCAAGCTCTTCTAA 12 0.3 No Hit TGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACC 12 0.3 No Hit GGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTTG 11 0.27499999999999997 No Hit ATTACGATCAAATCCACTGGTATCTCTCTTTTCTACGAGATGACTGATGA 11 0.27499999999999997 No Hit GCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTTGTTACTCACAGT 11 0.27499999999999997 No Hit GGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGG 11 0.27499999999999997 No Hit GGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGATTTT 11 0.27499999999999997 No Hit GAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACG 11 0.27499999999999997 No Hit AAACAAGGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTTGTTAC 10 0.25 No Hit CCTTGTGGCAGCTGCTGGGATTATTGCCCAGGAAGTTGCTGGTGATGTTC 10 0.25 No Hit CTTAAGATGCAGTACTATTTGCTCCTATCTGTTTGACATGCTGATATTTT 9 0.22499999999999998 No Hit GGTGATGTTCGCATGACTGATACCCGTGCAGATGAAGCAGAGCGTGGTAT 9 0.22499999999999998 No Hit CTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAA 9 0.22499999999999998 No Hit GTTCGCTCTTCGCGGAGACCACCGGCGAACCGTAGTCCCCAGCCATAGCC 9 0.22499999999999998 No Hit GACATCTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGA 9 0.22499999999999998 No Hit GCTGGTGATGTTCGCATGACTGATACCCGTGCAGATGAAGCAGAGCGTGG 9 0.22499999999999998 No Hit TTTGAGATGATTCACAGATTCCTTCTCGATCTATGCCCTCCAGAATCTGA 9 0.22499999999999998 No Hit GTATAGCTGGCTTCTACCGTGGGTGTGCCACAAATCTGTTGCGGACAACA 9 0.22499999999999998 No Hit TCTGAACAGCATCCCCAGCCACTGAAGCATTGACTGTATCGTTACTGTGT 9 0.22499999999999998 No Hit TGGACACTCCAATTGTTTCTGTTCATAGATCTATCATCTACAAATACTTC 9 0.22499999999999998 No Hit AAGCCTTTCGCTTACATGTTTTTTGTTTTTTTGCCGCCTTCACCATCTTC 8 0.2 No Hit GCTTGGTGTCACTCGAGAGGCCCATCCGGAGCTGACACCCCCCAGCCCTG 8 0.2 No Hit GTTTTTCCAACCATCATCGGGGACAATGTCACAGTAGGTCATAGTGCTGT 8 0.2 No Hit GTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCC 8 0.2 No Hit GGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTTGTTACTCACAG 8 0.2 No Hit GAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAA 8 0.2 No Hit GGTATCTCTCTTTTCTACGAGATGACTGATGAGTCACTCCAGATGTACAA 8 0.2 No Hit GATTATGCCTGCCTCGATGAGTATGGAGAGGAGAATCATATTGAAGGCTT 8 0.2 No Hit GGCAAATCTACGCTTACGGATTCCCTTGTGGCAGCTGCTGGGATTATTGC 8 0.2 No Hit GTTACTGTGTCCTATCCATGCTGTCATCCGAGAGTAGAAAACTAGAAATC 8 0.2 No Hit TCTCATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGAC 8 0.2 No Hit ATTGCCCAGGAAGTTGCTGGTGATGTTCGCATGACTGATACCCGTGCAGA 7 0.17500000000000002 No Hit GGCAAACTTAGAGGGAAAACATGTCTCCCAGAGCTCATAAAAGTGTTCAG 7 0.17500000000000002 No Hit ACCGGCGAACCGTAGTCCCCAGCCATAGCCACGAGCAAGGAAAGGAAAAA 7 0.17500000000000002 No Hit CAGACAGAAGCGTGGTGAAGATGTGGTGCACACCATGAAGGTTTCGTTAG 7 0.17500000000000002 No Hit GTTATGGTTTGTGATAAACTGGGAGATGGGAATGCTGTCGCAAACAAGTA 7 0.17500000000000002 No Hit AGCTGGCTTCTACCGTGGGTGTGCCACAAATCTGTTGCGGACAACACCTG 7 0.17500000000000002 No Hit CACAAATCTGTTGCGGACAACACCTGCTGCAGTTATAACCTTCACAAGCT 7 0.17500000000000002 No Hit CACAGATTCCTTCTCGATCTATGCCCTCCAGAATCTGAACAGCATCCCCA 7 0.17500000000000002 No Hit GTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACCTGATCA 7 0.17500000000000002 No Hit GAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATTG 7 0.17500000000000002 No Hit ACAAGGGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCA 7 0.17500000000000002 No Hit AGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCAT 7 0.17500000000000002 No Hit GACTGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAAT 7 0.17500000000000002 No Hit GCAACGCGCAGCCTGCCCCAAAGCATGAGCCGGCACAACTGAATTCAGGT 7 0.17500000000000002 No Hit GCCACAAATCTGTTGCGGACAACACCTGCTGCAGTTATAACCTTCACAAG 7 0.17500000000000002 No Hit GTTTACAGCAGAAGAGCTTCGCCGGATTATGGACAAGAAGAACAACATCC 7 0.17500000000000002 No Hit GAATCATATTGAAGGCTTTTGGCGCTGAACTGATCCTGACTGACCCACTC 7 0.17500000000000002 No Hit CCTTCACAAGCTTTGAGATGATTCACAGATTCCTTCTCGATCTATGCCCT 7 0.17500000000000002 No Hit GGTATAGCTGGCTTCTACCGTGGGTGTGCCACAAATCTGTTGCGGACAAC 7 0.17500000000000002 No Hit ATGGCAGCTTACAGATTATGACAAGATCATATTCATAGATGCCGATCTCC 7 0.17500000000000002 No Hit CATGTCTGTTATTGCTCATGTGGACCATGGCAAATCTACGCTTACGGATT 7 0.17500000000000002 No Hit CTCAAGAGCCACCGCCGCACGCGAACCGAGGATCAGTCGCTCGCGCTTCC 7 0.17500000000000002 No Hit TGTGCTCGATGAGGAGAAGCAGACGCTCAGCTTCACCGGGTATCTGCCCG 7 0.17500000000000002 No Hit ACGATCAAATCCACTGGTATCTCTCTTTTCTACGAGATGACTGATGAGTC 7 0.17500000000000002 No Hit AATAAGCCTTTCGCTTACATGTTTTTTGTTTTTTTGCCGCCTTCACCATC 6 0.15 No Hit GTTCTTCGTGGATTCAACGTCGACCAACCGAGGAATCTGGCAAAGAGTGT 6 0.15 No Hit GGAGAAGCAGACGCTCAGCTTCACCGGGTATCTGCCCGCCATGGGCTTCT 6 0.15 No Hit GATGTACAAGGGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTG 6 0.15 No Hit CGAAACAAGGCAAACCACAGAATCCCGAAGGAGAACAAAAAATCGTTGTT 6 0.15 No Hit GGGAAGATCGGATTGCATGTGAAGTTTTCTCTGCTCAATTATGATACAGA 6 0.15 No Hit TACGATCAAATCCACTGGTATCTCTCTTTTCTACGAGATGACTGATGAGT 6 0.15 No Hit CACTCTGAAGCAAGATATCGAGGGGTAATAGACTGTGTTAGAAAGGTTTA 6 0.15 No Hit ATGACTGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGA 6 0.15 No Hit CATGAATCTATGGTGCTTCAATGGGGTTTTCTGTTTGTGTTCCGCTGAGT 6 0.15 No Hit CGGACGCATGCCAAAGAAGACCAGCAACAACACCAAGTATTACGAGGTGC 6 0.15 No Hit GCCCAGGAAGTTGCTGGTGATGTTCGCATGACTGATACCCGTGCAGATGA 6 0.15 No Hit GGCAAAACTAGCTGCCTCAACCTTGACATATCCTCATGAGGTTGTTCGAT 6 0.15 No Hit ATAGACTGTGTTAGAAAGGTTTATCATGGAGAGGGTATAGCTGGCTTCTA 6 0.15 No Hit GGAAGATTGATGGCCTTATTTCTGGTATTGGAACAGGTGGTACCATTACC 6 0.15 No Hit AAAGATTCACTACGAGACAACTGGGCCTGAAATCTGGAGAAGTACCGGAG 6 0.15 No Hit CAGAAACAGTCAAGATGGTGAAGTTTACAGCAGAAGAGCTTCGCCGGATT 6 0.15 No Hit CAACAGTTCACGTGTTCTTGTCAATGGATTTGTTTGCAAAGACCCAAAGG 6 0.15 No Hit AGACATCTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAG 6 0.15 No Hit ATGTTGCCATTCAGTTCCCTGTATATGAGAAGATGAAAGCTTACCTGGCT 6 0.15 No Hit GATGAAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTT 6 0.15 No Hit CGAGGGGTAATAGACTGTGTTAGAAAGGTTTATCATGGAGAGGGTATAGC 6 0.15 No Hit GTGCAGATGAAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCT 6 0.15 No Hit GATGACTGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATG 6 0.15 No Hit CAGAAGAGCTTCGCCGGATTATGGACAAGAAGAACAACATCCGCAACATG 6 0.15 No Hit ACCAAATATTGCATTACTAACTGGTTCAACTTCCACGAGGGAGTCGAAGA 6 0.15 No Hit AATAGTAATTAACAGCGCACCTTCTCCTCTCATATTGTTCTCAGGTTTAG 6 0.15 No Hit AAGATCGGATTGCATGTGAAGTTTTCTCTGCTCAATTATGATACAGAGCA 6 0.15 No Hit ATCCGCAACATGTCTGTTATTGCTCATGTGGACCATGGCAAATCTACGCT 6 0.15 No Hit GCAAGATATCGAGGGGTAATAGACTGTGTTAGAAAGGTTTATCATGGAGA 6 0.15 No Hit CGAAGATAATTCGAAATGGTCTCAAGACTGGAGAAATAGCGATGGTCATC 6 0.15 No Hit GCCCTCCAGAATCTGAACAGCATCCCCAGCCACTGAAGCATTGACTGTAT 6 0.15 No Hit ATTGTAGATAGCGCAGGTTGTTGTTCATCTTTGTAGTTGTCAGGATTTTG 6 0.15 No Hit TGACTATTGACTAGTTCTCCACTTCTCTGGCTCTCAATAACAGGATCCAA 6 0.15 No Hit GTCGCTCGCGCTTCCTGTCGTATGCTCTTCCACCAGGCACCAGCGCGCCT 6 0.15 No Hit GCAAAGAGTGTGACTACACAGTAATTAGAGGGCAAACTTAGAGGGAAAAC 6 0.15 No Hit ACAGTGAGGAGTCACCAGCTGCTGATGCGGATGGCGGTGAGAAGAAAAAG 5 0.125 No Hit TTTTGATCAGAGAAACACAATGCTCAGTCTGCTTAGCAGACTATCAACCG 5 0.125 No Hit CTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACA 5 0.125 No Hit CTGCCCGCCATGGGCTTCTCCTGCAGCAACTTCAATTTCACTACCTAGGG 5 0.125 No Hit TCGCATGACTGATACCCGTGCAGATGAAGCAGAGCGTGGTATTACGATCA 5 0.125 No Hit GACAAGAAGAACAACATCCGCAACATGTCTGTTATTGCTCATGTGGACCA 5 0.125 No Hit GGTGTAAGGTGGTCACCAAGTGGGAAAACGTTGGCCTATGCAGGGCACAG 5 0.125 No Hit ACTCAGCTTACAAGAGTACTGTCGATTGCTTTGTGCAGACCTTAAAAAAT 5 0.125 No Hit ATCAACTCAGGATTTCAGGCTGCTTTCATGGTTCCAACTGAGATACTTGC 5 0.125 No Hit GCCACGGGCGCCGACAAGCCCGTGGGCTCGCCCCGGCCTGTGGCTATCAA 5 0.125 No Hit GCAGAATAACCACCCCAAGAAAGGAGACTGATACTTGCAAAATTCTTTCA 5 0.125 No Hit AAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATTGTTGTTCTCCT 5 0.125 No Hit GACCAAGCTGGTAAGAGCCAATCGGCTGGTCCATGGGGTGGTTCTGGTGG 5 0.125 No Hit AGATTGATGGCCTTATTTCTGGTATTGGAACAGGTGGTACCATTACCGGA 5 0.125 No Hit CATGTCTCCCAGAGCTCATAAAAGTGTTCAGATATTCTGTTGAAAAAAAT 5 0.125 No Hit TCTGGCAAAGAGTGTGACTACACAGTAATTAGAGGGCAAACTTAGAGGGA 5 0.125 No Hit TCTGTAATGGTGCTAACATTTATAACAGCTTATCTGAATGTGAGATCTGG 5 0.125 No Hit GGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAG 5 0.125 No Hit GTAGAACAGGGTAGCGAAGGCCTGACACATCAAGATGAAGAACCACGGAG 5 0.125 No Hit AGTTATCCTAGAATGTGGAATACTACTTACTGACTTGTCCGATCGCCAAT 5 0.125 No Hit GAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGATTTTTCTTC 5 0.125 No Hit CCTGACTGACCCACTCTTGGGAATGAAAGGAGCTATTCAGAAGGCAGAAG 5 0.125 No Hit AAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTTCTAC 5 0.125 No Hit GTTTTCAAAGATCTTGCCCTACCTCTATGATAAGGATGTAGTTAGTGAAG 5 0.125 No Hit GGTGTGTCCAAGACAGCAACCCCGGATGAGCTGAAGAAAGCTTATCGGAA 5 0.125 No Hit CTTGTGTTGCAGGTTCTGCCATTGGTAGTGATGGAAACATTATAAGAGCT 5 0.125 No Hit GCTTCGCCGGATTATGGACAAGAAGAACAACATCCGCAACATGTCTGTTA 5 0.125 No Hit ACTGAATTCAGGTGTTTACCCTGACGGACCACCCGTCCCTCTTCAGTCAA 5 0.125 No Hit CTCCAGAATCTGAACAGCATCCCCAGCCACTGAAGCATTGACTGTATCGT 5 0.125 No Hit AAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATTGT 5 0.125 No Hit AGGAGATGGAAATACTGTTGAAGTTTGAGGAAATGTGTCAAGAGACCACA 5 0.125 No Hit AGCTCTGTAGTTCATCCATCCACTAGTAGCTTCCCTGTGCTTCTTGCAAG 5 0.125 No Hit CTGGCATACAAGTATCCACTAAACCTGCAACTATAGATCAATTTACCTCT 5 0.125 No Hit GTCGACCAACCGAGGAATCTGGCAAAGAGTGTGACTACACAGTAATTAGA 5 0.125 No Hit GTGGATTCAACGTCGACCAACCGAGGAATCTGGCAAAGAGTGTGACTACA 5 0.125 No Hit CTTTTCTACGAGATGACTGATGAGTCACTCCAGATGTACAAGGGTGAGAG 5 0.125 No Hit GCCACTGAAGCATTGACTGTATCGTTACTGTGTCCTATCCATGCTGTCAT 5 0.125 No Hit GAAACAGTCAAGATGGTGAAGTTTACAGCAGAAGAGCTTCGCCGGATTAT 5 0.125 No Hit GAACAGCATCCCCAGCCACTGAAGCATTGACTGTATCGTTACTGTGTCCT 5 0.125 No Hit CAGATATCGTGTTGGTTGCTGCACGTTCATATGGTCTAACTGGTCTTGTC 5 0.125 No Hit GAAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTTCTA 5 0.125 No Hit AGCACACTCTGAAGCAAGATATCGAGGGGTAATAGACTGTGTTAGAAAGG 5 0.125 No Hit AGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGT 5 0.125 No Hit ATGATAGATACAAATCCTTCCTTCTTTTCCCAGTTCACTGTTGTCATTGC 5 0.125 No Hit AGGGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCT 5 0.125 No Hit AGATGATTCACAGATTCCTTCTCGATCTATGCCCTCCAGAATCTGAACAG 5 0.125 No Hit GAGAAGATGAAAGCTTACCTGGCTGAGCGAGACAATACTACTGTTGAAGC 5 0.125 No Hit GTTGCAGCTAGAGGCCCATCCCACCCACGTCTGCCGGTCTGCATCCATCT 5 0.125 No Hit AGTTTGTTCCTGAGTCATTGTATCGTGTGTTGAAACATTATAGCAATATG 5 0.125 No Hit GTTACTCTCAGTACAGGAACATCTTCCCTATCTGGGCGCTTGGTGAGTAC 5 0.125 No Hit ATTTGCTCGCCAAGATGAAGAATACGATTCCATGCTTGGTGTCACTCGAG 5 0.125 No Hit GGGAGTGTTACTGTCGTAGATGGCTCCAAAGTCGAACCATCAGACCTGGG 5 0.125 No Hit GGCACATGCTGTCGAGAACGCTAAGCCTTTGGAAAAGATTGAGTTTGAGA 5 0.125 No Hit AATTGTTTCTGTTCATAGATCTATCATCTACAAATACTTCTTGTGTTGCA 5 0.125 No Hit CAAGGGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCTGA 10 0.007233308 142.25 4 TCGGTCT 10 0.007233308 142.25 2 GTCGGTC 10 0.007233308 142.25 1 >>END_MODULE Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264772 spots for ERR5262800.sra Written 1264772 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra Read 1264754 spots for ERR5262800.sra Written 1264754 spots for ERR5262800.sra SRR ids: ['ERR5262800.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_pvcipam6 ERR5262800.sra spots: 25295098 blocks: [[1, 1264754], [1264755, 2529508], [2529509, 3794262], [3794263, 5059016], [5059017, 6323770], [6323771, 7588524], [7588525, 8853278], [8853279, 10118032], [10118033, 11382786], [11382787, 12647540], [12647541, 13912294], [13912295, 15177048], [15177049, 16441802], [16441803, 17706556], [17706557, 18971310], [18971311, 20236064], [20236065, 21500818], [21500819, 22765572], [22765573, 24030326], [24030327, 25295098]] ERR5262800 file size 8261055 ERR5262800 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262800 ERR5262800_1.fastq ERR5262800_2.fastq Input file: ERR5262800_1.fastq Paired file: ERR5262800_2.fastq trimmed: ERR5262800-trimmed-pair1.fastq, ERR5262800-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:02:15 2024 >> started Fri Dec 6 12:03:04 2024 >> done (48.600s) 25295098 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 3 ( 0.00%) empty read pairs filtered out after trimming by size control 25295095 (100.00%) read pairs available; of these: 8578 ( 0.03%) trimmed read pairs available after processing 25286517 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 2 0.00% 24 4 0.00% 25 2 0.00% 26 2 0.00% 27 4 0.00% 28 2 0.00% 29 1 0.00% 30 3 0.00% 31 6 0.00% 32 4 0.00% 33 6 0.00% 34 9 0.00% 35 9 0.00% 36 7 0.00% 37 8 0.00% 38 5 0.00% 39 5 0.00% 40 3 0.00% 41 2 0.00% 42 4 0.00% 43 3 0.00% 44 2 0.00% 45 6 0.00% 46 2 0.00% 47 1 0.00% 48 3 0.00% 49 642 0.00% 50 668 0.00% 51 748 0.00% 52 844 0.00% 53 962 0.00% 54 1062 0.00% 55 1261 0.00% 56 1338 0.01% 57 1484 0.01% 58 1756 0.01% 59 1993 0.01% 60 2404 0.01% 61 2650 0.01% 62 3171 0.01% 63 3403 0.01% 64 3621 0.01% 65 4101 0.02% 66 4422 0.02% 67 4954 0.02% 68 5586 0.02% 69 6050 0.02% 70 7119 0.03% 71 7607 0.03% 72 8677 0.03% 73 9404 0.04% 74 9816 0.04% 75 10611 0.04% 76 11610 0.05% 77 12496 0.05% 78 13271 0.05% 79 14105 0.06% 80 14904 0.06% 81 16325 0.06% 82 17288 0.07% 83 18796 0.07% 84 19706 0.08% 85 20980 0.08% 86 21275 0.08% 87 22551 0.09% 88 23542 0.09% 89 24199 0.10% 90 25894 0.10% 91 26972 0.11% 92 28611 0.11% 93 29866 0.12% 94 31773 0.13% 95 32627 0.13% 96 34303 0.14% 97 34419 0.14% 98 35477 0.14% 99 37125 0.15% 100 38438 0.15% 101 39681 0.16% 102 41295 0.16% 103 43053 0.17% 104 45049 0.18% 105 46162 0.18% 106 47881 0.19% 107 48687 0.19% 108 49943 0.20% 109 51120 0.20% 110 52028 0.21% 111 53620 0.21% 112 56730 0.22% 113 57797 0.23% 114 60318 0.24% 115 61339 0.24% 116 63080 0.25% 117 64434 0.25% 118 65404 0.26% 119 66436 0.26% 120 67363 0.27% 121 69287 0.27% 122 70887 0.28% 123 72246 0.29% 124 75151 0.30% 125 75930 0.30% 126 77432 0.31% 127 78244 0.31% 128 78652 0.31% 129 79724 0.32% 130 80861 0.32% 131 81511 0.32% 132 82881 0.33% 133 85875 0.34% 134 87137 0.34% 135 88137 0.35% 136 89923 0.36% 137 90593 0.36% 138 91244 0.36% 139 94412 0.37% 140 96971 0.38% 141 101190 0.40% 142 104965 0.41% 143 105539 0.42% 144 109472 0.43% 145 107871 0.43% 146 111239 0.44% 147 228966 0.91% 148 97874 0.39% 149 97914 0.39% 150 20788534 82.18% 25295095 reads passed initial QC criterion=sequence-density sequence-density=0.86 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=29 prefix-density=0.87 prefix-fanout=2.0 sequence=GACTCGACCTCGAGCGTGATGGTCTTGCCGGTGAGGGTCTTGACAAAGATCTGCATGCCACCACGCAGCCTGAGCACCA criterion=fanout-score sequence-density=0.02 sequence-density-rank=18 fanout-score=470.69 fanout-score-rank=1 prefix-density=0.64 prefix-fanout=16.2 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTGG criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=3.36 fanout-score-rank=21 prefix-density=0.62 prefix-fanout=3.1 sequence=ATCGACAACGTGAAGGC criterion=fanout-score sequence-density=0.04 sequence-density-rank=20 fanout-score=40.82 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=6.3 sequence=GATCAAGGAGGTTTCACACGAGTGGTCCTTGATTAACAAGCAGAAGCCTATCTGGATGAGGAAGCCTGAGGAAATTACCAAGGAGGAATATGCTGCTTTTTACAAGAGCCTGACCAACGACTGGGAGGAGCATCTTGCTGTCAAGCACTTCTCTGTGGAGGGGCAGCTTGAGTTCAAGGCGGTCCTCTTTGTCCCCAAGAGGGCCCCCTTTGACCTCTTTGACACCAAGAAGAAGGCCAACAACATCAAGCTCTATGTGCGCCGTGTCTTCATCATGGACAACTGTGAGGAGCTGATCCCAGAGTGGCTGGCCTTTGTCAAGGGTATTGTTGACTCTGAGGACCTTCCCCTCAACATCTCACGTGAGACCCTTCAGCAGAACAAGATCCTCAA ERR5262800 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:04:03 Started mapping on | Dec 06 12:04:13 Finished on | Dec 06 12:05:50 Mapping speed, Million of reads per hour | 938.79 Number of input reads | 25295095 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 24356296 Uniquely mapped reads % | 96.29% Average mapped length | 289.55 Number of splices: Total | 20185532 Number of splices: Annotated (sjdb) | 18709023 Number of splices: GT/AG | 19927881 Number of splices: GC/AG | 222249 Number of splices: AT/AC | 11878 Number of splices: Non-canonical | 23524 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.00% Deletion average length | 1.49 Insertion rate per base | 0.00% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 212031 % of reads mapped to multiple loci | 0.84% Number of reads mapped to too many loci | 1408 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.84% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 726768 726768 726768 N_multimapping 212031 212031 212031 N_noFeature 760386 23685012 996061 N_ambiguous 502794 3375 66802 UnstrandedReadsAssigned:23093116 PositiveStrandReadsAssigned:667909 NegativeStrandReadsAssigned:23293433 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262800 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262800-trimmed-pair1.fastq ERR5262800-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,295,095 reads, 23,720,597 reads pseudoaligned [quant] estimated average fragment length: 252.657 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,153 rounds 52973 ERR5262800.ke.tsv 35125 ERR5262800.se.tsv 88098 total ==> ERR5262800.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 685.996 0 0 PNS24247 1044 792.343 194.96 13.8319 PNS24249 1928 1676.34 485.808 16.2912 PNS24246 1044 792.343 194.96 13.8319 PNS24248 1044 792.343 194.96 13.8319 PNS24244 1471 1219.34 195.313 9.00443 PNS24243 293 101.117 1 0.555939 KQK14069 1603 1351.34 25101.2 1044.19 KQK14071 474 243.13 594.162 137.378 ==> ERR5262800.se.tsv <== BRADI_1g14170v3 27091 BRADI_1g53295v3 127 BRADI_1g59795v3 414 BRADI_1g07683v3 0 BRADI_1g00485v3 45 BRADI_1g20270v3 754 BRADI_1g74790v3 2476 BRADI_1g09890v3 0 BRADI_1g77505v3 231 BRADI_1g48960v3 0 ERR5262800 completed mapping pipeline successfully