Starting /dee2/code/volunteer_pipeline.sh ERR5262802 current disk space = 1551255220224 free memory = 1607254612 ERR5262802 SRAfilesize 0b59e6a09fbe0b5a2cc842bf9efad282 ERR5262802.sra ERR5262802.sra file validated ERR5262802 is paired end ERR5262802 is conventional basespace ERR5262802 read1 length is 71-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262802_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 71-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7925 37.0 37.0 37.0 37.0 37.0 2 36.5985 37.0 37.0 37.0 37.0 37.0 3 36.757 37.0 37.0 37.0 37.0 37.0 4 36.7495 37.0 37.0 37.0 37.0 37.0 5 36.7175 37.0 37.0 37.0 37.0 37.0 6 36.775 37.0 37.0 37.0 37.0 37.0 7 36.7735 37.0 37.0 37.0 37.0 37.0 8 36.7985 37.0 37.0 37.0 37.0 37.0 9 36.729 37.0 37.0 37.0 37.0 37.0 10-14 36.7197 37.0 37.0 37.0 37.0 37.0 15-19 36.683499999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.691 37.0 37.0 37.0 37.0 37.0 25-29 36.6332 37.0 37.0 37.0 37.0 37.0 30-34 36.6572 37.0 37.0 37.0 37.0 37.0 35-39 36.5815 37.0 37.0 37.0 37.0 37.0 40-44 36.5342 37.0 37.0 37.0 37.0 37.0 45-49 36.603899999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.6385 37.0 37.0 37.0 37.0 37.0 55-59 36.653499999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.6533 37.0 37.0 37.0 37.0 37.0 65-69 36.6174 37.0 37.0 37.0 37.0 37.0 70-74 36.656350662665666 37.0 37.0 37.0 37.0 37.0 75-79 36.626991023201796 37.0 37.0 37.0 37.0 37.0 80-84 36.54262320214406 37.0 37.0 37.0 37.0 37.0 85-89 36.62334034406476 37.0 37.0 37.0 37.0 37.0 90-94 36.63813075104002 37.0 37.0 37.0 37.0 37.0 95-99 36.50361774127576 37.0 37.0 37.0 37.0 37.0 100-104 36.537418855124635 37.0 37.0 37.0 37.0 37.0 105-109 36.586250668640716 37.0 37.0 37.0 37.0 37.0 110-114 36.42338651662267 37.0 37.0 37.0 37.0 37.0 115-119 36.3194238103919 37.0 37.0 37.0 37.0 37.0 120-124 36.37802807508199 37.0 37.0 37.0 37.0 37.0 125-129 36.280737756018056 37.0 37.0 37.0 37.0 37.0 130-134 36.3113124009499 37.0 37.0 37.0 37.0 37.0 135-139 36.28346221479788 37.0 37.0 37.0 37.0 37.0 140-144 36.284797773018774 37.0 37.0 37.0 37.0 37.0 145-149 36.360508817957076 37.0 37.0 37.0 37.0 37.0 150 36.15824508320726 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 5.0 29 6.0 30 12.0 31 19.0 32 32.0 33 29.0 34 71.0 35 192.0 36 2953.0 37 681.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.949999999999996 6.15 10.525 31.374999999999996 2 17.508754377188595 15.407703851925964 50.0 17.083541770885443 3 14.099999999999998 20.075000000000003 33.35 32.475 4 27.425 25.7 24.525 22.35 5 14.35 31.900000000000002 28.000000000000004 25.75 6 24.75 27.525 28.525 19.2 7 13.05 22.975 42.725 21.25 8 17.224999999999998 38.1 16.275000000000002 28.4 9 12.7 27.325 37.65 22.325 10-14 15.2 44.335 20.09 20.375 15-19 18.135 36.19 21.525 24.15 20-24 24.485 33.515 23.544999999999998 18.455 25-29 20.035 38.275 23.485 18.205 30-34 17.78 32.029999999999994 31.195 18.995 35-39 17.8 35.144999999999996 27.625 19.43 40-44 23.75 30.685000000000002 25.455 20.11 45-49 21.82 35.589999999999996 24.104999999999997 18.485 50-54 24.125 38.145 21.075 16.655 55-59 18.085 34.48 24.485 22.95 60-64 13.605 43.21 20.21 22.975 65-69 18.82 41.13 16.89 23.16 70-74 15.462319347902184 30.519577936690506 27.784167625143773 26.23393509026354 75-79 16.92938998148426 33.32832907971776 20.507431316619126 29.234849622178853 80-84 22.265173954515788 36.24679953812942 18.243887745368742 23.244138761986044 85-89 18.848826669352402 32.45039782455434 21.175344949138886 27.525430556954376 90-94 20.364296886825773 27.514001715525506 21.928452495080478 30.193248902568243 95-99 19.144706661909375 34.19644222437433 21.203934961007185 25.454916152709107 100-104 20.634757600657355 37.977608874281024 15.119145439605589 26.26848808545604 105-109 28.351796252200025 32.405010870690546 18.83217724402112 20.41101563308831 110-114 22.157724087438552 33.75692919150716 23.104277795209708 20.981068925844575 115-119 26.36886847246423 35.4084164950631 13.1210729183167 25.101642114155975 120-124 30.393725992317545 42.41357234314981 10.782116944088775 16.410584720443875 125-129 18.083211915169176 43.6889536452431 14.122281582213589 24.10555285737413 130-134 19.796647430612808 32.728771640560595 20.131904369332236 27.342676559494368 135-139 21.33370692070608 28.982908377696837 26.472401232838326 23.210983468758755 140-144 24.905074214704868 24.174433321827177 22.88574387297204 28.034748590495916 145-149 21.498100664767332 31.024453941120605 24.85161443494777 22.625830959164293 150 15.400907715582452 36.88350983358548 18.245083207261725 29.470499243570348 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.5 28 1.0 29 0.5 30 0.5 31 6.5 32 13.0 33 21.0 34 45.0 35 94.5 36 106.0 37 94.0 38 415.5 39 434.5 40 79.0 41 14.5 42 173.5 43 598.5 44 744.0 45 404.5 46 160.5 47 99.0 48 137.5 49 217.0 50 121.5 51 15.0 52 3.0 53 1.5 54 2.5 55 3.0 56 4.0 57 2.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70-71 1.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 11.0 80-81 3.0 82-83 5.0 84-85 8.0 86-87 2.0 88-89 1.0 90-91 2.0 92-93 9.0 94-95 26.0 96-97 15.0 98-99 8.0 100-101 17.0 102-103 5.0 104-105 9.0 106-107 22.0 108-109 15.0 110-111 18.0 112-113 12.0 114-115 14.0 116-117 16.0 118-119 21.0 120-121 9.0 122-123 14.0 124-125 22.0 126-127 18.0 128-129 28.0 130-131 24.0 132-133 36.0 134-135 21.0 136-137 32.0 138-139 25.0 140-141 56.0 142-143 45.0 144-145 36.0 146-147 35.0 148-149 53.0 150-151 3305.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.125 #Duplication Level Percentage of deduplicated Percentage of total 1 38.63013698630137 3.5249999999999995 2 17.80821917808219 3.25 3 10.684931506849315 2.9250000000000003 4 6.027397260273973 2.1999999999999997 5 3.5616438356164384 1.625 6 2.4657534246575343 1.35 7 1.095890410958904 0.7000000000000001 8 1.36986301369863 1.0 9 1.095890410958904 0.8999999999999999 >10 12.602739726027398 23.3 >50 1.643835616438356 11.700000000000001 >100 3.0136986301369864 47.525 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATAT 367 9.175 No Hit GTGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATG 259 6.4750000000000005 No Hit CAAGCGTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTGGGAAGCA 222 5.55 No Hit GTCAAGCGTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTGGGAAG 171 4.275 No Hit GCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAAC 142 3.55 No Hit TGTTTGTCAAGCGTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTG 133 3.325 No Hit GTTGTTTGTCAAGCGTCGAAGATGTACAGCACTGCACAAAACATCTGTCT 129 3.225 No Hit CTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACA 125 3.125 No Hit GGCTGCTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACG 123 3.075 No Hit GCTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACGGGGA 118 2.9499999999999997 No Hit GTCAACAACAATAACAATGTGACTTCTTAACTACACATAAGAATGTTGTT 112 2.8000000000000003 No Hit CGTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATA 93 2.325 No Hit CTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAACAAC 87 2.175 No Hit GCTGCTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACGG 83 2.075 No Hit CACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAACA 74 1.8499999999999999 No Hit GTTTGTCAAGCGTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTGG 68 1.7000000000000002 No Hit CGAAGATGTACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGT 63 1.575 No Hit GACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTA 50 1.25 No Hit GTACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACA 50 1.25 No Hit CTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGCA 49 1.225 No Hit CTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGCACTGCA 45 1.125 No Hit TTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAG 41 1.0250000000000001 No Hit CCAAAAACTACTTATGTAAATGTCAACAACAATAACAATGTGACTTCTTA 38 0.95 No Hit CTTATGTAAATGTCAACAACAATAACAATGTGACTTCTTAACTACACATA 37 0.9249999999999999 No Hit CAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACA 37 0.9249999999999999 No Hit GTTGCATTTACATCTGGTCCTTACTATTTGTTTAATCCTTCCCGCTTAGG 34 0.8500000000000001 No Hit CGTTTGTCAAGCGTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTG 32 0.8 No Hit ACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAACAA 30 0.75 No Hit CTGCTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACGGG 25 0.625 No Hit CACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGCACTGCACAA 23 0.575 No Hit CTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACGGGGAC 21 0.525 No Hit AGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAA 20 0.5 No Hit ACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGCACTGC 20 0.5 No Hit GCGGCCTTTGGCTGGTCCATCGGTACCATGTGACCAGAATCATGGACCTT 19 0.475 No Hit TACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGCACTGCAC 18 0.44999999999999996 No Hit CTCAGAAATCTGTCGTGAGAGCACAGTAGTAGCATCAAGATGAGCGAAAG 17 0.42500000000000004 No Hit GTAAATGTCAACAACAATAACAATGTGACTTCTTAACTACACATAAGAAT 16 0.4 No Hit CGTGAGAGCACAGTAGTAGCATCAAGATGAGCGAAAGTTGTAGCAGGTGC 16 0.4 No Hit TGTGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGAT 15 0.375 No Hit ACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAAC 14 0.35000000000000003 No Hit ACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGCACTGCACA 14 0.35000000000000003 No Hit GTCGTGAGAGCACAGTAGTAGCATCAAGATGAGCGAAAGTTGTAGCAGGT 13 0.325 No Hit GCCTTTGGCTGGTCCATCGGTACCATGTGACCAGAATCATGGACCTTCAA 13 0.325 No Hit GGACCATAGCTTTTTAGAACACCGGCTTCTTTTCCATCAACTGTGAACGG 13 0.325 No Hit GTGGCTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATG 13 0.325 No Hit GGTTTGTCAAGCGTCGAAGATGTACAGCACTGCACAAAACATCTGTCTTG 12 0.3 No Hit CTACTTATGTAAATGTCAACAACAATAACAATGTGACTTCTTAACTACAC 12 0.3 No Hit CAACAACAATAACAATGTGACTTCTTAACTACACATAAGAATGTTGTTTG 12 0.3 No Hit CAACAATAACAATGTGACTTCTTAACTACACATAAGAATGTTGTTTGTCA 12 0.3 No Hit CCAGAATCATGGACCTTCAAGAAACTCAAAGGACCATAGCTTTTTAGAAC 12 0.3 No Hit GCTTTTTAGAACACCGGCTTCTTTTCCATCAACTGTGAACGGCTCCTCAG 11 0.27499999999999997 No Hit ATGTGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGA 11 0.27499999999999997 No Hit CTCCAAGGCGGCCTTTGGCTGGTCCATCGGTACCATGTGACCAGAATCAT 11 0.27499999999999997 No Hit CAAAAACTACTTATGTAAATGTCAACAACAATAACAATGTGACTTCTTAA 11 0.27499999999999997 No Hit TGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAACAACA 11 0.27499999999999997 No Hit GGCCTTTGGCTGGTCCATCGGTACCATGTGACCAGAATCATGGACCTTCA 11 0.27499999999999997 No Hit CGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGT 11 0.27499999999999997 No Hit GGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAA 11 0.27499999999999997 No Hit GACCATAGCTTTTTAGAACACCGGCTTCTTTTCCATCAACTGTGAACGGC 11 0.27499999999999997 No Hit GCTCAGAAATCTGTCGTGAGAGCACAGTAGTAGCATCAAGATGAGCGAAA 10 0.25 No Hit AATGTGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAG 10 0.25 No Hit CCTTTGGCTGGTCCATCGGTACCATGTGACCAGAATCATGGACCTTCAAG 10 0.25 No Hit GACCAGAATCATGGACCTTCAAGAAACTCAAAGGACCATAGCTTTTTAGA 10 0.25 No Hit GGCTGGTCCATCGGTACCATGTGACCAGAATCATGGACCTTCAAGAAACT 9 0.22499999999999998 No Hit CGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAACAA 9 0.22499999999999998 No Hit CTCTCCTCCATTGGAACAAACGGTGATCCTGCCATGTAACGCAAGTGCAA 9 0.22499999999999998 No Hit AAGATGTACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTAT 9 0.22499999999999998 No Hit TCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGC 8 0.2 No Hit CAGAAATCTGTCGTGAGAGCACAGTAGTAGCATCAAGATGAGCGAAAGTT 8 0.2 No Hit ATAGCTTTTTAGAACACCGGCTTCTTTTCCATCAACTGTGAACGGCTCCT 8 0.2 No Hit CTCATAACATGAAATTATCAGTTTTACCTCTTATAGGCTGAAAATAACTG 8 0.2 No Hit TGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGT 8 0.2 No Hit GATGTACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGT 7 0.17500000000000002 No Hit GGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGT 7 0.17500000000000002 No Hit AACAATAACAATGTGACTTCTTAACTACACATAAGAATGTTGTTTGTCAA 7 0.17500000000000002 No Hit CATCGGTACCATGTGACCAGAATCATGGACCTTCAAGAAACTCAAAGGAC 7 0.17500000000000002 No Hit TGCTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACGGGG 6 0.15 No Hit TCGAAGATGTACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATG 6 0.15 No Hit TTTTTTTTTTTTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTA 6 0.15 No Hit CGTGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGAT 6 0.15 No Hit TTTTTTTTTAAATGTCAACAACAATAACAATGTGACTTCTTAACTACACA 6 0.15 No Hit TTTTTTTTTTTTTTCAACAACAATAACAATGTGACTTCTTAACTACACAT 6 0.15 No Hit TTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTACAGCAC 6 0.15 No Hit CTGTCGTGAGAGCACAGTAGTAGCATCAAGATGAGCGAAAGTTGTAGCAG 6 0.15 No Hit AGACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGT 6 0.15 No Hit TACAGCACTGCACAAAACATCTGTCTTGGGAAGCATATATGTATGTACAA 5 0.125 No Hit CTTTGGCTGGTCCATCGGTACCATGTGACCAGAATCATGGACCTTCAAGA 5 0.125 No Hit AAAAACTACTTATGTAAATGTCAACAACAATAACAATGTGACTTCTTAAC 5 0.125 No Hit TTTTTTTTTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAG 5 0.125 No Hit GGACCAACTTGTTGCATTTACATCTGGTCCTTACTATTTGTTTAATCCTT 5 0.125 No Hit GCACTTACGATAAGCTCATAACATGAAATTATCAGTTTTACCTCTTATAG 5 0.125 No Hit CGGTACCATGTGACCAGAATCATGGACCTTCAAGAAACTCAAAGGACCAT 5 0.125 No Hit AACTACTTATGTAAATGTCAACAACAATAACAATGTGACTTCTTAACTAC 5 0.125 No Hit GCCGCTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACGG 5 0.125 No Hit CCTGGTTGGTAGTCCAATACAAGTGTTATCTTACTACTCATCTTTATCCA 5 0.125 No Hit ACTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGTAC 5 0.125 No Hit GTTGCTTCACAGAATAACCGAAGGGAAATAAGTCAAAACAAATTGCACGG 5 0.125 No Hit TGGCTTCTTAACTACACATAAGAATGTTGTTTGTCAAGCGTCGAAGATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTGCT 10 0.007480128 140.66249 1 GTCAACA 30 0.0020275437 70.33125 1 CAACAAT 30 0.0020275437 70.33125 6 TCAACAA 30 0.0020275437 70.33125 2 AACAATA 35 0.0037360555 60.283924 7 CAATAAC 35 0.0037360555 60.283924 9 ACAATAA 35 0.0037360555 60.283924 8 TGACTTC 65 1.0876769E-5 54.100964 2 GTGACTT 65 1.0876769E-5 54.100964 1 ACTTCTT 70 1.6871809E-5 50.236603 4 GACTTCT 70 1.6871809E-5 50.236603 3 TTCTTAA 95 1.8279407E-6 44.419735 6 CTTCTTA 95 1.8279407E-6 44.419735 5 TCTTAAC 95 1.8279407E-6 44.419735 7 CTTAACT 100 2.602752E-6 42.198746 8 TTAACTA 100 2.602752E-6 42.198746 9 GAATAAC 40 0.009119682 35.165623 9 CCTTCAC 30 1.3695111E-5 34.100002 140-144 GTTTGTG 35 9.845953E-7 34.1 140-144 TTCACAA 20 0.002671701 34.1 140-144 >>END_MODULE ERR5262802 read2 length is 71-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262802_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 71-150 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2295 37.0 37.0 37.0 37.0 37.0 2 36.1985 37.0 37.0 37.0 37.0 37.0 3 36.2845 37.0 37.0 37.0 37.0 37.0 4 36.3325 37.0 37.0 37.0 37.0 37.0 5 36.391 37.0 37.0 37.0 37.0 37.0 6 36.287 37.0 37.0 37.0 37.0 37.0 7 36.403 37.0 37.0 37.0 37.0 37.0 8 36.4085 37.0 37.0 37.0 37.0 37.0 9 36.4265 37.0 37.0 37.0 37.0 37.0 10-14 36.3635 37.0 37.0 37.0 37.0 37.0 15-19 36.34159999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.342600000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.3243 37.0 37.0 37.0 37.0 37.0 30-34 36.296800000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.2089 37.0 37.0 37.0 37.0 37.0 40-44 36.2271 37.0 37.0 37.0 37.0 37.0 45-49 36.198 37.0 37.0 37.0 37.0 37.0 50-54 36.099599999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.0632 37.0 37.0 37.0 37.0 37.0 60-64 35.9951 37.0 37.0 37.0 37.0 37.0 65-69 36.0077 37.0 37.0 37.0 37.0 37.0 70-74 36.015153063265814 37.0 37.0 37.0 37.0 37.0 75-79 35.99113129931992 37.0 37.0 37.0 37.0 37.0 80-84 35.85290240468512 37.0 37.0 37.0 37.0 37.0 85-89 35.86611345523487 37.0 37.0 37.0 37.0 37.0 90-94 35.73463733897128 37.0 37.0 37.0 37.0 37.0 95-99 35.74791859073504 37.0 37.0 37.0 37.0 37.0 100-104 35.681096458904754 37.0 37.0 37.0 37.0 37.0 105-109 35.56059883358869 37.0 37.0 37.0 37.0 37.0 110-114 35.657939096905444 37.0 37.0 37.0 37.0 37.0 115-119 35.60973340306938 37.0 37.0 37.0 37.0 37.0 120-124 35.588639501208604 37.0 37.0 37.0 37.0 37.0 125-129 35.534258333376705 37.0 37.0 37.0 37.0 37.0 130-134 35.45890743448131 37.0 37.0 37.0 34.6 37.0 135-139 35.40165108745501 37.0 37.0 37.0 34.6 37.0 140-144 35.39951740826206 37.0 37.0 37.0 34.6 37.0 145-149 35.388414725293714 37.0 37.0 37.0 32.2 37.0 150 35.29404617253949 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 2.0 13 2.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 0.0 21 0.0 22 2.0 23 5.0 24 5.0 25 7.0 26 9.0 27 6.0 28 14.0 29 21.0 30 25.0 31 32.0 32 40.0 33 98.0 34 229.0 35 606.0 36 2571.0 37 320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.525000000000006 24.025 9.675 22.775000000000002 2 30.099999999999998 29.45 24.525 15.925 3 26.400000000000002 32.125 23.125 18.35 4 27.275 33.95 16.6 22.175 5 28.849999999999998 36.8 15.7 18.65 6 24.175 42.775 15.625 17.424999999999997 7 23.5 28.375 26.825 21.3 8 28.9 27.6 21.475 22.025 9 26.05 29.099999999999998 23.799999999999997 21.05 10-14 28.875 29.794999999999998 21.060000000000002 20.27 15-19 28.04 28.23 23.785 19.945 20-24 27.47 26.51 23.724999999999998 22.295 25-29 25.979999999999997 28.494999999999997 24.285 21.240000000000002 30-34 27.46 25.91 25.069999999999997 21.560000000000002 35-39 26.090000000000003 26.665 24.7 22.545 40-44 25.53 27.37 24.845 22.255 45-49 23.974999999999998 27.694999999999997 25.88 22.45 50-54 25.929999999999996 26.06 25.525 22.485 55-59 26.87 25.71 23.225 24.195 60-64 27.935 27.295 23.91 20.86 65-69 27.750000000000004 26.584999999999997 24.7 20.965 70-74 27.774166124918736 24.788718307746162 26.063909586437966 21.373205980897133 75-79 28.464194565380573 25.34654456287845 27.273182204874143 18.916078666866838 80-84 25.749284602640692 24.243184898840305 28.691199357397462 21.31633114112154 85-89 25.78809547789304 25.621915600765433 27.303857387450904 21.28613153389062 90-94 25.57142136333821 27.413088450476813 27.27180987940865 19.743680306776326 95-99 25.89836383098017 27.534532850807892 26.525307100259955 20.041796217951983 100-104 24.912695152013146 24.75862777321282 28.245686113393592 22.082990961380446 105-109 23.74469406770887 28.724505642406044 26.638368361113983 20.892431928771092 110-114 25.112435937663424 28.020081581424538 25.405292333437924 21.462190147474114 115-119 25.85669781931464 26.590633085168168 27.48825175563652 20.06441733988067 120-124 26.317755014938115 22.87131882202305 28.1423388817755 22.66858728126334 125-129 26.188548918029248 25.217203604770383 29.29685392045761 19.297393556742755 130-134 25.908416249793852 29.959870265515914 26.595569237535045 17.536144247155185 135-139 25.030860733924364 25.44607788127034 29.28964201548648 20.23341936931882 140-144 25.298288085768633 23.419217245950776 30.08242550002882 21.200069168251773 145-149 25.403538030853532 23.074632199654534 28.89391863720293 22.627911132289 150 22.691373025516405 25.759416767922232 30.164034021871206 21.38517618469016 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 1.5 24 1.5 25 0.5 26 0.5 27 1.0 28 1.5 29 1.0 30 2.0 31 3.0 32 4.0 33 4.0 34 5.5 35 8.0 36 11.0 37 13.0 38 38.5 39 57.5 40 48.5 41 50.5 42 140.0 43 298.5 44 493.5 45 450.0 46 343.0 47 357.0 48 242.5 49 129.0 50 71.5 51 57.5 52 82.5 53 88.0 54 127.5 55 153.0 56 135.0 57 144.0 58 93.5 59 37.0 60 49.5 61 54.0 62 51.0 63 57.5 64 48.0 65 32.0 66 10.5 67 5.5 68 3.5 69 2.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70-71 1.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 11.0 80-81 3.0 82-83 5.0 84-85 8.0 86-87 2.0 88-89 1.0 90-91 2.0 92-93 9.0 94-95 26.0 96-97 15.0 98-99 8.0 100-101 17.0 102-103 5.0 104-105 9.0 106-107 22.0 108-109 15.0 110-111 18.0 112-113 12.0 114-115 14.0 116-117 16.0 118-119 21.0 120-121 9.0 122-123 14.0 124-125 22.0 126-127 18.0 128-129 28.0 130-131 25.0 132-133 36.0 134-135 25.0 136-137 32.0 138-139 26.0 140-141 57.0 142-143 45.0 144-145 42.0 146-147 35.0 148-149 53.0 150-151 3292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.075 #Duplication Level Percentage of deduplicated Percentage of total 1 42.782969885773625 10.299999999999999 2 16.510903426791277 7.95 3 10.176531671858775 7.35 4 7.78816199376947 7.5 5 4.361370716510903 5.25 6 2.907580477673936 4.2 7 1.453790238836968 2.45 8 1.557632398753894 3.0 9 1.557632398753894 3.375 >10 10.488058151609552 42.55 >50 0.4153686396677051 6.075 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGTG 73 1.825 No Hit CAAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTC 59 1.4749999999999999 No Hit GCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACC 58 1.4500000000000002 No Hit ATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCC 53 1.325 No Hit AAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCT 47 1.175 No Hit GGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAG 38 0.95 No Hit GAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCAT 36 0.8999999999999999 No Hit CCCGTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGTTGT 34 0.8500000000000001 No Hit GGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACG 34 0.8500000000000001 No Hit GATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTC 33 0.8250000000000001 No Hit GTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCAACTC 33 0.8250000000000001 No Hit GTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGTTGTACATACATATAT 31 0.775 No Hit TGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGT 31 0.775 No Hit GTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGTTGTACATACATAT 30 0.75 No Hit GGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAA 29 0.7250000000000001 No Hit AAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTT 29 0.7250000000000001 No Hit TGAAGAACAAGGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAAGCA 29 0.7250000000000001 No Hit CACAAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTT 28 0.7000000000000001 No Hit GTCACAAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGT 26 0.65 No Hit GAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAA 26 0.65 No Hit GTCTGTTGTTGTTGTACATACATATATGCTTCCCAAGACAGATGTTTTGT 26 0.65 No Hit GCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAG 23 0.575 No Hit CGAGAACGTGGAGAACCATGCTCTTGTGAAGGACGGCAAGCTTGCTAATC 22 0.5499999999999999 No Hit GAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCA 22 0.5499999999999999 No Hit AGGCGAAGCGCGGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAG 21 0.525 No Hit GAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCAA 21 0.525 No Hit AGGAGATGGCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACC 20 0.5 No Hit GAAGAACAAGGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAAGCAA 20 0.5 No Hit CCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGTGCAGTGT 20 0.5 No Hit AGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGTTGTACATACATA 19 0.475 No Hit TGGTCACAAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCC 18 0.44999999999999996 No Hit GTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGTTGTACA 18 0.44999999999999996 No Hit GCTCAACTGAGGAAGATTGAGGAGCAATTGGAAAAGAAGAAGGCTGAATA 18 0.44999999999999996 No Hit TGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAA 17 0.42500000000000004 No Hit GAGATGGCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCAT 17 0.42500000000000004 No Hit GTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTT 17 0.42500000000000004 No Hit CCGAGATCGGTGTTCCACTGCTTCGATGGCGAGAACGTGGAGAACCATGC 17 0.42500000000000004 No Hit GGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTG 17 0.42500000000000004 No Hit TTCACCCGTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTG 17 0.42500000000000004 No Hit GGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGA 17 0.42500000000000004 No Hit AGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGT 16 0.4 No Hit AAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCAAGGCCGAGGAG 16 0.4 No Hit GGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCA 16 0.4 No Hit AGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCAAGG 16 0.4 No Hit AAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCCGAG 16 0.4 No Hit CGCAGAGAAGATGAAGAACAAGGCTGCAATGATCCACAAGGAGGCCGAAG 16 0.4 No Hit AAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATC 15 0.375 No Hit CCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGT 15 0.375 No Hit CCTGTGTGTAAAGTCTGTTGTTGTTGTACATACATATATGCTTCCCAAGA 15 0.375 No Hit GAACAAGGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAAGCAATGG 15 0.375 No Hit GAACAAGGCTGCTAAGAAGGTGTCCGCCATTCTCTCGTGGGAGAACACCA 14 0.35000000000000003 No Hit GTGTGCCCTGTGTGTAAAGTCTGTTGTTGTTGTACATACATATATGCTTC 14 0.35000000000000003 No Hit CAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCC 14 0.35000000000000003 No Hit GGCGAAGCGCGGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGT 14 0.35000000000000003 No Hit CTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGTGCAGTGTGCCCTGTGT 14 0.35000000000000003 No Hit GTAAAGTCTGTTGTTGTTGTACATACATATATGCTTCCCAAGACAGATGT 14 0.35000000000000003 No Hit GTTTTCTTTCACCCGTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCT 14 0.35000000000000003 No Hit AAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTC 13 0.325 No Hit GTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGC 13 0.325 No Hit CTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCAACTCTCC 13 0.325 No Hit GGCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCAATGATCCACAAGG 13 0.325 No Hit ATGGCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGG 13 0.325 No Hit CTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAAC 13 0.325 No Hit CACCAAGAAAGCAAACATAGAAGCTCAACTGAGGAAGATTGAGGAGCAAT 13 0.325 No Hit GAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGG 13 0.325 No Hit TCACAAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTT 13 0.325 No Hit GAAGAAGTTGCTGTCTCTGCCGGGTCGCCGAGATCGGTGTTCCACTGCTT 13 0.325 No Hit CCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACA 13 0.325 No Hit AAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCAAG 13 0.325 No Hit AGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGG 12 0.3 No Hit GTGTAAAGTCTGTTGTTGTTGTACATACATATATGCTTCCCAAGACAGAT 12 0.3 No Hit TGGAAAAGAAGAAGGCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCA 12 0.3 No Hit CGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCT 12 0.3 No Hit AGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTG 12 0.3 No Hit AGGAGAATGAGAAGACAAAGGCTGAGAACAAGGCTGCTAAGAAGGTGTCC 12 0.3 No Hit GGAAAAGAAGAAGGCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCAA 12 0.3 No Hit GCGCGGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCG 12 0.3 No Hit GCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAA 12 0.3 No Hit GTTGCTGTCTCTGCCGGGTCGCCGAGATCGGTGTTCCACTGCTTCGATGG 12 0.3 No Hit AGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCAACT 11 0.27499999999999997 No Hit GTGGGAGAACACCAAGAAAGCAAACATAGAAGCTCAACTGAGGAAGATTG 11 0.27499999999999997 No Hit CGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGC 11 0.27499999999999997 No Hit GTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTG 11 0.27499999999999997 No Hit AAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGTTGTACATACA 11 0.27499999999999997 No Hit GAGCAATTGGAAAAGAAGAAGGCTGAATACGCAGAGAAGATGAAGAACAA 11 0.27499999999999997 No Hit GGCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGT 11 0.27499999999999997 No Hit GCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGTGCAGTGTGCCCTGTG 11 0.27499999999999997 No Hit AGCAATTGGAAAAGAAGAAGGCTGAATACGCAGAGAAGATGAAGAACAAG 11 0.27499999999999997 No Hit CTGGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAA 11 0.27499999999999997 No Hit AGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCA 11 0.27499999999999997 No Hit GTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGTGCAGTGTGCCCT 10 0.25 No Hit GCTTGCTAATCTGCTCCAATGGCGAGAGGACGCTCTGGAGGAGGATGGCG 10 0.25 No Hit AAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCCGAGGT 10 0.25 No Hit GTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGG 10 0.25 No Hit GAGAACACCAAGAAAGCAAACATAGAAGCTCAACTGAGGAAGATTGAGGA 10 0.25 No Hit CACCCGTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGTT 10 0.25 No Hit TTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCA 10 0.25 No Hit GCGAAGCGCGGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTA 10 0.25 No Hit GGAGATGGCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCA 10 0.25 No Hit AGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGC 10 0.25 No Hit CGGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCA 10 0.25 No Hit CTCAAGATGGGTAAACTCTATGGAATGGTCTGGAAAGAAAGCATTTGTGT 10 0.25 No Hit GATCCACAAGGAGGCCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCG 10 0.25 No Hit GTGCCCTGTGTGTAAAGTCTGTTGTTGTTGTACATACATATATGCTTCCC 10 0.25 No Hit AGAAGCTCAACTGAGGAAGATTGAGGAGCAATTGGAAAAGAAGAAGGCTG 10 0.25 No Hit CCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTC 9 0.22499999999999998 No Hit GCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCAATGATCCACAAGGA 9 0.22499999999999998 No Hit TAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGT 9 0.22499999999999998 No Hit CGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCA 9 0.22499999999999998 No Hit GAGAAGAGAAACTCTTTGATTAAAGCATGGGAGGAGAATGAGAAGACAAA 9 0.22499999999999998 No Hit CATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAA 9 0.22499999999999998 No Hit CCGCCATTCTCTCGTGGGAGAACACCAAGAAAGCAAACATAGAAGCTCAA 9 0.22499999999999998 No Hit ACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAA 9 0.22499999999999998 No Hit ATCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGTGCAGT 9 0.22499999999999998 No Hit GGCGAGAACGTGGAGAACCATGCTCTTGTGAAGGACGGCAAGCTTGCTAA 9 0.22499999999999998 No Hit GAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCAAGGCCG 9 0.22499999999999998 No Hit AGTTGCTGTCTCTGCCGGGTCGCCGAGATCGGTGTTCCACTGCTTCGATG 9 0.22499999999999998 No Hit AAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCAAC 9 0.22499999999999998 No Hit CCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCT 9 0.22499999999999998 No Hit CAAGGAGGCCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGG 9 0.22499999999999998 No Hit GCTCCCGTGTGGCGGTGTTCTCATTTACCAGAGCTTTGCTGTGAACGAAG 8 0.2 No Hit CGAAGAAGTTGCTGTCTCTGCCGGGTCGCCGAGATCGGTGTTCCACTGCT 8 0.2 No Hit CTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTG 8 0.2 No Hit AGGAAACTCAAGATGGGTAAACTCTATGGAATGGTCTGGAAAGAAAGCAT 8 0.2 No Hit TGGCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGG 8 0.2 No Hit CATACATATATGCTTCCCAAGACAGATGTTTTGTGCAGTGCTGTACATCT 8 0.2 No Hit AGAAGGCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCAATGATCCAC 8 0.2 No Hit GAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCAA 8 0.2 No Hit CCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTC 8 0.2 No Hit AAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCCGA 8 0.2 No Hit TCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTT 8 0.2 No Hit GGGAGTTGGAGACATAGAGTTTGTTTCATGCAGCCCGACTGTCTACCAGG 8 0.2 No Hit TCCGAGGTTGCTTTCTTCCGTTTTCTTTCACCCGTCCAAAGTGTGCAGTG 8 0.2 No Hit GAGAAGACAAAGGCTGAGAACAAGGCTGCTAAGAAGGTGTCCGCCATTCT 8 0.2 No Hit AAAGTCTGTTGTTGTTGTACATACATATATGCTTCCCAAGACAGATGTTT 8 0.2 No Hit GATGAAGAACAAGGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAAG 7 0.17500000000000002 No Hit ATGATCCACAAGGAGGCCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCG 7 0.17500000000000002 No Hit CAAGGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAAGCAATGGTCG 7 0.17500000000000002 No Hit AAGAAGGCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCAATGATCCA 7 0.17500000000000002 No Hit GCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCTT 7 0.17500000000000002 No Hit GGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCT 7 0.17500000000000002 No Hit AAGAGCTCCCGTGTGGCGGTGTTCTCATTTACCAGAGCTTTGCTGTGAAC 7 0.17500000000000002 No Hit AAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCCG 7 0.17500000000000002 No Hit AGAACAAGGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAAGCAATG 7 0.17500000000000002 No Hit CTTGAGAGCGACATCAAAGTGCTGATTTATGCTGGAGAGTATGATCTCAT 7 0.17500000000000002 No Hit TTTCACCCGTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTT 7 0.17500000000000002 No Hit GGAGGAGAATGAGAAGACAAAGGCTGAGAACAAGGCTGCTAAGAAGGTGT 7 0.17500000000000002 No Hit GAGGCGAAGCGCGGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAA 7 0.17500000000000002 No Hit GCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAA 7 0.17500000000000002 No Hit CGGTGTTCCACTGCTTCGATGGCGAGAACGTGGAGAACCATGCTCTTGTG 6 0.15 No Hit TGAACGAAGAAGTTGCTGTCTCTGCCGGGTCGCCGAGATCGGTGTTCCAC 6 0.15 No Hit GGTTGAGCAAGAGCTCCCGTGTGGCGGTGTTCTCATTTACCAGAGCTTTG 6 0.15 No Hit GGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCAC 6 0.15 No Hit GTCCGCCATTCTCTCGTGGGAGAACACCAAGAAAGCAAACATAGAAGCTC 6 0.15 No Hit GAAGACAAAGGCTGAGAACAAGGCTGCTAAGAAGGTGTCCGCCATTCTCT 6 0.15 No Hit AACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCA 6 0.15 No Hit GGACGCTCTGGAGGAGGATGGCGTGCTGTACGAGTGATCTGATGTCTGCT 6 0.15 No Hit CCACAAGGAGGCCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGG 6 0.15 No Hit GAATGAGAAGACAAAGGCTGAGAACAAGGCTGCTAAGAAGGTGTCCGCCA 6 0.15 No Hit AGAAGACAAAGGCTGAGAACAAGGCTGCTAAGAAGGTGTCCGCCATTCTC 6 0.15 No Hit CGACTGTCTACCAGGCTATGCTCTTAGATTGGATGAGGAACCTTGAAGTT 6 0.15 No Hit CACAAGGAGGCCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGA 6 0.15 No Hit AAGATTGAGGAGCAATTGGAAAAGAAGAAGGCTGAATACGCAGAGAAGAT 6 0.15 No Hit AAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTC 6 0.15 No Hit GGAGAATGAGAAGACAAAGGCTGAGAACAAGGCTGCTAAGAAGGTGTCCG 6 0.15 No Hit CTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAG 6 0.15 No Hit AAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCC 6 0.15 No Hit GTATGATCTCATATGCAATTGGCTAGGAAACTCAAGATGGGTAAACTCTA 6 0.15 No Hit AGGCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCAATGATCCACAAG 6 0.15 No Hit GCCATTCTCTCGTGGGAGAACACCAAGAAAGCAAACATAGAAGCTCAACT 6 0.15 No Hit GCCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCT 6 0.15 No Hit CTTTCACCCGTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGT 6 0.15 No Hit AAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCAAGGCCGAGGA 6 0.15 No Hit AGGAGGCCGAAGAGAAGAAAGCAATGGTCGAGGCGAAGCGCGGGGAGGAA 6 0.15 No Hit GAAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCA 6 0.15 No Hit GGTCGAGGCGAAGCGCGGGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGG 6 0.15 No Hit GTTTTGTTCCAACATTCGGGGCTGAAAAGTTATTTGTTGGATAAAGATGA 6 0.15 No Hit GGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGA 5 0.125 No Hit CACATCTCATGTGCTAAGGAGATGACAAAGGTCTCTGGTATCTCTGAAAT 5 0.125 No Hit AACGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTT 5 0.125 No Hit GCGGCCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTG 5 0.125 No Hit TGGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAA 5 0.125 No Hit GAATTTTCAACAGATTTAGAGCGCACAATTTAACAATTCAGCCTTTGCAC 5 0.125 No Hit AATCACTGAATTTTCAACAGATTTAGAGCGCACAATTTAACAATTCAGCC 5 0.125 No Hit CCAAGTACCGCGCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCTTT 5 0.125 No Hit ATTCCATCTGCTGTGGGATACCAACCAACCCTTGCTACTGATCTGGGAGG 5 0.125 No Hit CGCCGAGATCGGTGTTCCACTGCTTCGATGGCGAGAACGTGGAGAACCAT 5 0.125 No Hit CTGAATTTTCAACAGATTTAGAGCGCACAATTTAACAATTCAGCCTTTGC 5 0.125 No Hit CCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACTGGCAACTCTC 5 0.125 No Hit GAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGG 5 0.125 No Hit AGATGAAGAACAAGGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAA 5 0.125 No Hit CGAGCAAGATTGAAGTGTTCATCCGAGGTTGCTTTCTTCCGTTTTCTTTC 5 0.125 No Hit CATAGAAGCTCAACTGAGGAAGATTGAGGAGCAATTGGAAAAGAAGAAGG 5 0.125 No Hit AGAACACCAAGAAAGCAAACATAGAAGCTCAACTGAGGAAGATTGAGGAG 5 0.125 No Hit TCACCCGTCCAAAGTGTGCAGTGTGCCCTGTGTGTAAAGTCTGTTGTTGT 5 0.125 No Hit CTCAAATGACAGAGATATTGCTCTTGCAAGGGTGGAAACTGAGAAGAGAA 5 0.125 No Hit GGCTGCAATGATCCACAAGGAGGCCGAAGAGAAGAAAGCAATGGTCGAGG 5 0.125 No Hit ATTGGAAAAGAAGAAGGCTGAATACGCAGAGAAGATGAAGAACAAGGCTG 5 0.125 No Hit CTTATATGTCCAAAAATGGCATGTTCAGCGGCCTGTTCCTAGCGTTTGTT 5 0.125 No Hit GGAAACTCAAGATGGGTAAACTCTATGGAATGGTCTGGAAAGAAAGCATT 5 0.125 No Hit AGCAAACATAGAAGCTCAACTGAGGAAGATTGAGGAGCAATTGGAAAAGA 5 0.125 No Hit CAGAGATATTGCTCTTGCAAGGGTGGAAACTGAGAAGAGAAACTCTTTGA 5 0.125 No Hit GAAAGCATTTGTGTCCTCAACTGAGGAGCCGTTCACAGTTGATGGAAAAG 5 0.125 No Hit GGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGATTG 5 0.125 No Hit GGGAGGAAGTCCTCAAGGCCGAGGAGATGGCGGCCAAGTACCGCGCCACT 5 0.125 No Hit AGGATGTGCTTCTCTTCATTGATAACATTTTCCGTTTCACTCAGGCAAAC 5 0.125 No Hit CTGGAAAGAAAGCATTTGTGTCCTCAACTGAGGAGCCGTTCACAGTTGAT 5 0.125 No Hit AGGAGCAATTGGAAAAGAAGAAGGCTGAATACGCAGAGAAGATGAAGAAC 5 0.125 No Hit GAAAAGAAGAAGGCTGAATACGCAGAGAAGATGAAGAACAAGGCTGCAAT 5 0.125 No Hit TTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCACAAACGAGCAAGAT 5 0.125 No Hit GCCACTGGCAACTCTCCCAAGAAAACCATGGGGTGCTTTGGGGCCTAAAG 5 0.125 No Hit CTGTGAACGAAGAAGTTGCTGTCTCTGCCGGGTCGCCGAGATCGGTGTTC 5 0.125 No Hit CGTGGGAGAACACCAAGAAAGCAAACATAGAAGCTCAACTGAGGAAGATT 5 0.125 No Hit CTATGGAATGGTCTGGAAAGAAAGCATTTGTGTCCTCAACTGAGGAGCCG 5 0.125 No Hit AAACGTTCCTTGGTCACAAACGAGCAAGATTGAAGTGTTCATCCGAGGTT 5 0.125 No Hit AAGCAAACATAGAAGCTCAACTGAGGAAGATTGAGGAGCAATTGGAAAAG 5 0.125 No Hit CTGAAATCATCCCGGAGATGGAGGTCTGTGACTTCGACTTTGAGCCCTGC 5 0.125 No Hit AAGAAAACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTT 5 0.125 No Hit ACCATGGGGTGCTTTGGGGCCTAAAGAAAAAAAAAACGTTCCTTGGTCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGTG 120 0.0012541284 10.967836 135-139 AAGACAG 125 0.0027143802 10.002667 125-129 TTCCCAA 125 0.0043610507 9.451339 120-124 TATGCTT 130 0.0065723 8.993406 115-119 GTTGTTG 345 0.008702874 5.4362316 125-129 >>END_MODULE Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460167 spots for ERR5262802.sra Written 1460167 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra Read 1460162 spots for ERR5262802.sra Written 1460162 spots for ERR5262802.sra SRR ids: ['ERR5262802.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_486encfb ERR5262802.sra spots: 29203245 blocks: [[1, 1460162], [1460163, 2920324], [2920325, 4380486], [4380487, 5840648], [5840649, 7300810], [7300811, 8760972], [8760973, 10221134], [10221135, 11681296], [11681297, 13141458], [13141459, 14601620], [14601621, 16061782], [16061783, 17521944], [17521945, 18982106], [18982107, 20442268], [20442269, 21902430], [21902431, 23362592], [23362593, 24822754], [24822755, 26282916], [26282917, 27743078], [27743079, 29203245]] ERR5262802 file size 9507442 ERR5262802 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262802 ERR5262802_1.fastq ERR5262802_2.fastq Input file: ERR5262802_1.fastq Paired file: ERR5262802_2.fastq trimmed: ERR5262802-trimmed-pair1.fastq, ERR5262802-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:02:56 2024 >> started Fri Dec 6 12:03:39 2024 >> done (42.800s) 29203245 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 29203244 (100.00%) read pairs available; of these: 8590 ( 0.03%) trimmed read pairs available after processing 29194654 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 4 0.00% 26 1 0.00% 27 1 0.00% 28 3 0.00% 29 1 0.00% 30 1 0.00% 31 2 0.00% 32 2 0.00% 33 2 0.00% 34 0 0.00% 35 3 0.00% 36 5 0.00% 37 4 0.00% 38 6 0.00% 39 11 0.00% 40 2 0.00% 41 1 0.00% 42 1 0.00% 43 0 0.00% 44 3 0.00% 45 2 0.00% 46 2 0.00% 47 0 0.00% 48 2 0.00% 49 435 0.00% 50 444 0.00% 51 522 0.00% 52 614 0.00% 53 597 0.00% 54 663 0.00% 55 764 0.00% 56 809 0.00% 57 983 0.00% 58 1000 0.00% 59 1181 0.00% 60 1497 0.01% 61 1606 0.01% 62 1892 0.01% 63 2000 0.01% 64 2324 0.01% 65 2472 0.01% 66 2908 0.01% 67 3092 0.01% 68 3531 0.01% 69 4120 0.01% 70 4674 0.02% 71 5310 0.02% 72 6191 0.02% 73 7081 0.02% 74 7762 0.03% 75 8536 0.03% 76 9350 0.03% 77 10298 0.04% 78 11374 0.04% 79 12772 0.04% 80 14077 0.05% 81 15106 0.05% 82 17515 0.06% 83 19411 0.07% 84 21112 0.07% 85 23132 0.08% 86 24429 0.08% 87 26641 0.09% 88 27841 0.10% 89 29935 0.10% 90 31697 0.11% 91 34472 0.12% 92 36859 0.13% 93 39486 0.14% 94 43275 0.15% 95 45097 0.15% 96 47116 0.16% 97 49277 0.17% 98 50577 0.17% 99 52421 0.18% 100 54933 0.19% 101 56465 0.19% 102 59422 0.20% 103 62820 0.22% 104 65069 0.22% 105 67855 0.23% 106 69948 0.24% 107 71353 0.24% 108 72924 0.25% 109 74942 0.26% 110 75521 0.26% 111 77207 0.26% 112 81729 0.28% 113 81952 0.28% 114 86053 0.29% 115 88493 0.30% 116 90289 0.31% 117 92157 0.32% 118 94239 0.32% 119 94182 0.32% 120 93960 0.32% 121 97141 0.33% 122 98243 0.34% 123 100386 0.34% 124 103832 0.36% 125 105253 0.36% 126 106079 0.36% 127 108316 0.37% 128 109530 0.38% 129 110531 0.38% 130 111203 0.38% 131 111124 0.38% 132 112292 0.38% 133 114179 0.39% 134 115738 0.40% 135 116887 0.40% 136 119568 0.41% 137 119559 0.41% 138 120944 0.41% 139 122724 0.42% 140 122835 0.42% 141 124160 0.43% 142 125948 0.43% 143 125807 0.43% 144 128352 0.44% 145 128728 0.44% 146 132022 0.45% 147 241959 0.83% 148 125524 0.43% 149 125657 0.43% 150 23340872 79.93% 29203244 reads passed initial QC criterion=sequence-density sequence-density=2.34 sequence-density-rank=1 fanout-score=2.58 fanout-score-rank=29 prefix-density=2.71 prefix-fanout=2.2 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.04 sequence-density-rank=32 fanout-score=194.96 fanout-score-rank=1 prefix-density=1.57 prefix-fanout=4.4 sequence=CCGCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=8.50 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=29 prefix-density=8.47 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.36 sequence-density-rank=21 fanout-score=52.52 fanout-score-rank=1 prefix-density=8.83 prefix-fanout=2.2 sequence=GTTCCGGTTCGCGGCTAGCAGTAGTTGTTGTAGTAGCAGCTAGGGTTTCCGGTAGGGTTCCGTCGAGATCGCCATGGATGAGTACCGCTGCTTCGTGGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262802 ERR5262802_1.fastq ERR5262802_2.fastq Input file: ERR5262802_1.fastq Paired file: ERR5262802_2.fastq trimmed: ERR5262802-trimmed-pair1.fastq, ERR5262802-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:06:42 2024 >> started Fri Dec 6 12:07:07 2024 >> done (25.060s) 19468829 read pairs processed; of these: 136 ( 0.00%) short read pairs filtered out after trimming by size control 271 ( 0.00%) empty read pairs filtered out after trimming by size control 19468422 (100.00%) read pairs available; of these: 167 ( 0.00%) trimmed read pairs available after processing 19468255 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 25 4 0.00% 26 0 0.00% 27 1 0.00% 28 2 0.00% 29 1 0.00% 30 0 0.00% 31 1 0.00% 32 2 0.00% 33 1 0.00% 34 0 0.00% 35 3 0.00% 36 3 0.00% 37 3 0.00% 38 4 0.00% 39 8 0.00% 40 1 0.00% 41 1 0.00% 42 1 0.00% 43 0 0.00% 44 2 0.00% 45 0 0.00% 46 1 0.00% 47 0 0.00% 48 1 0.00% 49 202 0.00% 50 204 0.00% 51 330 0.00% 52 318 0.00% 53 295 0.00% 54 345 0.00% 55 404 0.00% 56 446 0.00% 57 535 0.00% 58 561 0.00% 59 655 0.00% 60 959 0.00% 61 1026 0.01% 62 1411 0.01% 63 1455 0.01% 64 1706 0.01% 65 1808 0.01% 66 2157 0.01% 67 2292 0.01% 68 2398 0.01% 69 2785 0.01% 70 3390 0.02% 71 3874 0.02% 72 3658 0.02% 73 4555 0.02% 74 5930 0.03% 75 6026 0.03% 76 5487 0.03% 77 7572 0.04% 78 7256 0.04% 79 9238 0.05% 80 8637 0.04% 81 10792 0.06% 82 10913 0.06% 83 12243 0.06% 84 15197 0.08% 85 14863 0.08% 86 16791 0.09% 87 17167 0.09% 88 18455 0.09% 89 20679 0.11% 90 20798 0.11% 91 21551 0.11% 92 23606 0.12% 93 25939 0.13% 94 27603 0.14% 95 30910 0.16% 96 33627 0.17% 97 31839 0.16% 98 32536 0.17% 99 35994 0.18% 100 35058 0.18% 101 37625 0.19% 102 38786 0.20% 103 42671 0.22% 104 43641 0.22% 105 44886 0.23% 106 47057 0.24% 107 47816 0.25% 108 49803 0.26% 109 48944 0.25% 110 50986 0.26% 111 50852 0.26% 112 54664 0.28% 113 55073 0.28% 114 57812 0.30% 115 57717 0.30% 116 60653 0.31% 117 60811 0.31% 118 62286 0.32% 119 62481 0.32% 120 62977 0.32% 121 64519 0.33% 122 66138 0.34% 123 66758 0.34% 124 69468 0.36% 125 69994 0.36% 126 70774 0.36% 127 72243 0.37% 128 72667 0.37% 129 73852 0.38% 130 74480 0.38% 131 73939 0.38% 132 74702 0.38% 133 75738 0.39% 134 77002 0.40% 135 77739 0.40% 136 79585 0.41% 137 79579 0.41% 138 80866 0.42% 139 82303 0.42% 140 82035 0.42% 141 83059 0.43% 142 83910 0.43% 143 84564 0.43% 144 85411 0.44% 145 85787 0.44% 146 88277 0.45% 147 160609 0.82% 148 83566 0.43% 149 83694 0.43% 150 15563107 79.94% criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=4.37 fanout-score-rank=32 prefix-density=0.37 prefix-fanout=3.3 sequence=GTGATGGTCTTGCC criterion=fanout-score sequence-density=0.04 sequence-density-rank=34 fanout-score=348.35 fanout-score-rank=1 prefix-density=0.60 prefix-fanout=23.7 sequence=CGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=2.17 fanout-score-rank=32 prefix-density=0.67 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=28 fanout-score=877.88 fanout-score-rank=1 prefix-density=0.91 prefix-fanout=25.0 sequence=GCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGC ERR5262802 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:08:06 Started mapping on | Dec 06 12:08:06 Finished on | Dec 06 12:10:04 Mapping speed, Million of reads per hour | 890.93 Number of input reads | 29202837 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 28547120 Uniquely mapped reads % | 97.75% Average mapped length | 288.60 Number of splices: Total | 24376257 Number of splices: Annotated (sjdb) | 22512207 Number of splices: GT/AG | 24057336 Number of splices: GC/AG | 276105 Number of splices: AT/AC | 16889 Number of splices: Non-canonical | 25927 Mismatch rate per base, % | 0.11% Deletion rate per base | 0.00% Deletion average length | 1.53 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 241296 % of reads mapped to multiple loci | 0.83% Number of reads mapped to too many loci | 1143 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.40% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 414421 414421 414421 N_multimapping 241296 241296 241296 N_noFeature 1260696 27732540 1531195 N_ambiguous 630618 3845 86825 UnstrandedReadsAssigned:26655806 PositiveStrandReadsAssigned:810735 NegativeStrandReadsAssigned:26929100 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262802 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262802-trimmed-pair1.fastq ERR5262802-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,202,837 reads, 27,201,248 reads pseudoaligned [quant] estimated average fragment length: 245.146 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,180 rounds 52973 ERR5262802.ke.tsv 35125 ERR5262802.se.tsv 88098 total ==> ERR5262802.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 692.811 0 0 PNS24247 1044 799.854 200.527 13.5916 PNS24249 1928 1683.85 505.057 16.261 PNS24246 1044 799.854 200.527 13.5916 PNS24248 1044 799.854 200.527 13.5916 PNS24244 1471 1226.85 278.364 12.3007 PNS24243 293 110.002 0 0 KQK14069 1603 1358.85 21915.4 874.352 KQK14071 474 251.996 753.952 162.204 ==> ERR5262802.se.tsv <== BRADI_1g14170v3 25226 BRADI_1g53295v3 201 BRADI_1g59795v3 974 BRADI_1g07683v3 0 BRADI_1g00485v3 25 BRADI_1g20270v3 983 BRADI_1g74790v3 2623 BRADI_1g09890v3 0 BRADI_1g77505v3 230 BRADI_1g48960v3 0 ERR5262802 completed mapping pipeline successfully