Starting /dee2/code/volunteer_pipeline.sh ERR5262803 current disk space = 1551039225856 free memory = 1603730264 ERR5262803 SRAfilesize 1a8bab8e02291df5cbc8aa1ceefb7b07 ERR5262803.sra ERR5262803.sra file validated ERR5262803 is paired end ERR5262803 is conventional basespace ERR5262803 read1 length is 65-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262803_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 65-150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2475 37.0 37.0 37.0 37.0 37.0 2 36.45125 37.0 37.0 37.0 37.0 37.0 3 36.561 37.0 37.0 37.0 37.0 37.0 4 36.6835 37.0 37.0 37.0 37.0 37.0 5 36.6635 37.0 37.0 37.0 37.0 37.0 6 36.594 37.0 37.0 37.0 37.0 37.0 7 36.6205 37.0 37.0 37.0 37.0 37.0 8 36.645 37.0 37.0 37.0 37.0 37.0 9 36.671 37.0 37.0 37.0 37.0 37.0 10-14 36.6633 37.0 37.0 37.0 37.0 37.0 15-19 36.6438 37.0 37.0 37.0 37.0 37.0 20-24 36.6295 37.0 37.0 37.0 37.0 37.0 25-29 36.66459999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.67809999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.629 37.0 37.0 37.0 37.0 37.0 40-44 36.6777 37.0 37.0 37.0 37.0 37.0 45-49 36.6422 37.0 37.0 37.0 37.0 37.0 50-54 36.581900000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.569 37.0 37.0 37.0 37.0 37.0 60-64 36.571400000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.497599924981245 37.0 37.0 37.0 37.0 37.0 70-74 36.47376333700638 37.0 37.0 37.0 37.0 37.0 75-79 36.531410069063305 37.0 37.0 37.0 37.0 37.0 80-84 36.55814997434821 37.0 37.0 37.0 37.0 37.0 85-89 36.51325990808043 37.0 37.0 37.0 37.0 37.0 90-94 36.466525246607745 37.0 37.0 37.0 37.0 37.0 95-99 36.44268520160369 37.0 37.0 37.0 37.0 37.0 100-104 36.49859867242316 37.0 37.0 37.0 37.0 37.0 105-109 36.51681442443549 37.0 37.0 37.0 37.0 37.0 110-114 36.420879027047256 37.0 37.0 37.0 37.0 37.0 115-119 36.493876619779066 37.0 37.0 37.0 37.0 37.0 120-124 36.470258687420596 37.0 37.0 37.0 37.0 37.0 125-129 36.420300648375715 37.0 37.0 37.0 37.0 37.0 130-134 36.39301482275483 37.0 37.0 37.0 37.0 37.0 135-139 36.363655745658185 37.0 37.0 37.0 37.0 37.0 140-144 36.41190014340895 37.0 37.0 37.0 37.0 37.0 145-149 36.35005921419631 37.0 37.0 37.0 37.0 37.0 150 36.310491206791994 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 0.0 26 0.0 27 2.0 28 2.0 29 6.0 30 11.0 31 21.0 32 38.0 33 61.0 34 91.0 35 202.0 36 2546.0 37 1019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.175 27.125 2.175 49.525000000000006 2 6.129597197898424 29.622216662496875 22.041531148361273 42.20665499124343 3 8.525 29.875 22.225 39.375 4 14.75 36.5 17.525 31.225 5 12.9 44.2 14.325 28.575 6 19.0 48.0 20.225 12.775 7 9.25 31.55 39.65 19.55 8 13.675 41.449999999999996 22.625 22.25 9 21.0 32.475 26.875 19.650000000000002 10-14 16.155 41.760000000000005 20.919999999999998 21.165 15-19 9.725 39.36 22.945 27.97 20-24 12.905 34.175 22.27 30.65 25-29 15.485 35.885 20.77 27.860000000000003 30-34 15.325 35.125 16.04 33.51 35-39 20.3 28.58 19.470000000000002 31.65 40-44 20.71 27.565 17.215 34.510000000000005 45-49 15.39 38.64 15.64 30.330000000000002 50-54 20.395 33.36 20.495 25.75 55-59 18.655 29.659999999999997 21.705 29.98 60-64 27.560000000000002 26.02 11.594999999999999 34.825 65-69 18.87377475495099 28.395679135827166 22.829565913182638 29.900980196039207 70-74 18.632110872066843 28.088257367288737 27.122629709311052 26.157002051333368 75-79 21.96366911875094 37.882199869889405 16.549066706700696 23.60506430465896 80-84 21.321246118401284 35.8509466092357 15.115696684363417 27.7121105879996 85-89 20.980283951236643 38.12772788842623 14.287864345557619 26.604123814779513 90-94 15.552873736611858 37.00910142304018 20.882988887212754 26.555035953135214 95-99 17.84217700812844 32.18054223254405 24.42065936285152 25.556621396475993 100-104 15.194686676130603 28.330967349422025 27.524842831068746 28.949503143378625 105-109 15.410539215686276 30.443218954248362 20.818014705882355 33.32822712418301 110-114 22.993604291314217 32.00949040643697 16.123375283680627 28.87353001856819 115-119 22.6957745011202 29.26066795185745 18.006564893450737 30.036992653571616 120-124 20.871498914597343 28.659924816011014 17.82178217821782 32.646794091173824 125-129 22.24132373482325 27.500805845062853 18.582787149457396 31.675083270656497 130-134 24.72852912142152 32.25841833936602 16.151146210376222 26.861906328836238 135-139 21.59923363011383 21.954243209737406 18.46613321311845 37.98038994703032 140-144 35.01517667945708 13.607468071702652 17.98866044327358 33.38869480556669 145-149 26.08926360474029 16.56152349507694 26.714226755497904 30.634986144684866 150 19.43602183141298 18.859915100060643 23.86294724075197 37.84111582777441 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 1.0 28 2.5 29 2.0 30 4.5 31 4.0 32 28.5 33 170.0 34 168.0 35 26.5 36 1.5 37 2.5 38 1.0 39 7.0 40 24.0 41 84.5 42 243.0 43 297.0 44 179.0 45 77.0 46 197.0 47 281.0 48 182.5 49 91.0 50 181.5 51 658.5 52 751.0 53 298.5 54 37.0 55 0.5 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 64-65 1.0 66-67 0.0 68-69 0.0 70-71 2.0 72-73 0.0 74-75 0.0 76-77 1.0 78-79 1.0 80-81 1.0 82-83 4.0 84-85 1.0 86-87 3.0 88-89 4.0 90-91 4.0 92-93 7.0 94-95 8.0 96-97 3.0 98-99 10.0 100-101 2.0 102-103 13.0 104-105 2.0 106-107 28.0 108-109 14.0 110-111 10.0 112-113 17.0 114-115 14.0 116-117 23.0 118-119 28.0 120-121 24.0 122-123 18.0 124-125 28.0 126-127 14.0 128-129 27.0 130-131 46.0 132-133 31.0 134-135 50.0 136-137 25.0 138-139 21.0 140-141 22.0 142-143 31.0 144-145 40.0 146-147 51.0 148-149 73.0 150-151 3298.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.55 #Duplication Level Percentage of deduplicated Percentage of total 1 39.76608187134503 3.4000000000000004 2 11.695906432748536 2.0 3 7.894736842105263 2.025 4 5.263157894736842 1.7999999999999998 5 5.555555555555555 2.375 6 2.3391812865497075 1.2 7 2.923976608187134 1.7500000000000002 8 2.3391812865497075 1.6 9 1.461988304093567 1.125 >10 14.912280701754385 28.4 >50 4.093567251461988 25.825 >100 1.7543859649122806 28.499999999999996 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAAT 334 8.35 No Hit CCATCTTATAAGAATCTCACTTGATTGCTCGACTACATATGAACTGCACT 199 4.9750000000000005 No Hit CTTGAATTGCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGGC 196 4.9 No Hit CACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAA 167 4.175 No Hit CACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACAT 122 3.05 No Hit CCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAATG 122 3.05 No Hit AAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAATGGC 96 2.4 No Hit GCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGGCGCAACACA 94 2.35 No Hit CACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATACAC 92 2.3 No Hit CACCCTTCCACATTGATAATCCTTGAATTGCTGGATGTCTCGCGCTAGCT 90 2.25 No Hit CCCTTCCACATTGATAATCCTTGAATTGCTGGATGTCTCGCGCTAGCTTG 87 2.175 No Hit ATCGAAAAAACGTTACAACACCAAGTAGTACAACACATACACAGCAACAA 73 1.825 No Hit CATCTTATAAGAATCTCACTTGATTGCTCGACTACATATGAACTGCACTA 70 1.7500000000000002 No Hit ACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCA 68 1.7000000000000002 No Hit GTTACAACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCT 65 1.625 No Hit AAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATACACAGC 64 1.6 No Hit ACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATACACA 61 1.525 No Hit ACAACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAA 61 1.525 No Hit GCACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCA 58 1.4500000000000002 No Hit ATCTTATAAGAATCTCACTTGATTGCTCGACTACATATGAACTGCACTAG 54 1.35 No Hit CAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACAAATA 50 1.25 No Hit CAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAATGG 47 1.175 No Hit CCTTGAATTGCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGG 45 1.125 No Hit ACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATACA 44 1.0999999999999999 No Hit CTCAATTGTTCTATCTTTTCCACTTGTTTTCCAGTAACCATTGGGTGTGG 43 1.075 No Hit CTACATTCTTCGGCCAGCAAACTTGATCGCCATAATCTCAGGGACAGACG 41 1.0250000000000001 No Hit CAACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAAC 39 0.975 No Hit GCAACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAAC 34 0.8500000000000001 No Hit GTTCTACATTCTTCGGCCAGCAAACTTGATCGCCATAATCTCAGGGACAG 33 0.8250000000000001 No Hit GGCGCAACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTAC 31 0.775 No Hit CTTATAAGAATCTCACTTGATTGCTCGACTACATATGAACTGCACTAGAA 30 0.75 No Hit CACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATAC 29 0.7250000000000001 No Hit GTTCAGTTCTACATTCTTCGGCCAGCAAACTTGATCGCCATAATCTCAGG 29 0.7250000000000001 No Hit GTTCAGCTCAATTGTTCTATCTTTTCCACTTGTTTTCCAGTAACCATTGG 28 0.7000000000000001 No Hit CAGCTCAATTGTTCTATCTTTTCCACTTGTTTTCCAGTAACCATTGGGTG 26 0.65 No Hit GCTCAATTGTTCTATCTTTTCCACTTGTTTTCCAGTAACCATTGGGTGTG 26 0.65 No Hit TACAACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCA 24 0.6 No Hit GCGCAACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACA 24 0.6 No Hit AACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACC 24 0.6 No Hit GTACCATCCAAATACTGTTCTGCTCCTGCAATTTCAGTGTCTTCATGAAT 23 0.575 No Hit GCCATAATCTCAGGGACAGACGTTAACAAACAAATAGAAAAAACGATACA 23 0.575 No Hit CTACAGTTCAGTTCTACATTCTTCGGCCAGCAAACTTGATCGCCATAATC 22 0.5499999999999999 No Hit CATCGAAAAAACGTTACAACACCAAGTAGTACAACACATACACAGCAACA 21 0.525 No Hit CTTCGGCCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAA 18 0.44999999999999996 No Hit GGACAGACGTTAACAAACAAATAGAAAAAACGATACACGGCGCAGTCAAA 18 0.44999999999999996 No Hit CCAACAGTTCGAGAGTTCAGCTCAATTGTTCTATCTTTTCCACTTGTTTT 18 0.44999999999999996 No Hit GGCCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACAA 17 0.42500000000000004 No Hit GCCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAAT 17 0.42500000000000004 No Hit GCCCTTCCACATTGATAATCCTTGAATTGCTGGATGTCTCGCGCTAGCTT 17 0.42500000000000004 No Hit ACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATA 17 0.42500000000000004 No Hit CCTTCCACATTGATAATCCTTGAATTGCTGGATGTCTCGCGCTAGCTTGC 16 0.4 No Hit GAATTGCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGGCGCA 16 0.4 No Hit CCCAACAGTTCGAGAGTTCAGCTCAATTGTTCTATCTTTTCCACTTGTTT 16 0.4 No Hit CCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACAAAT 15 0.375 No Hit CTCAGGGACAGACGTTAACAAACAAATAGAAAAAACGATACACGGCGCAG 15 0.375 No Hit ACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACA 14 0.35000000000000003 No Hit GTTGTACCATCCAAATACTGTTCTGCTCCTGCAATTTCAGTGTCTTCATG 14 0.35000000000000003 No Hit GCTTGCTCACGCCCGCGCGCAGGCGCAACACACACAAAACATCGAAAAAA 14 0.35000000000000003 No Hit AGCTTGCTCACGCCCGCGCGCAGGCGCAACACACACAAAACATCGAAAAA 14 0.35000000000000003 No Hit CAACAGTTCGAGAGTTCAGCTCAATTGTTCTATCTTTTCCACTTGTTTTC 14 0.35000000000000003 No Hit CAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATACACAG 13 0.325 No Hit GCAACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAA 13 0.325 No Hit GTTCGAGAGTTCAGCTCAATTGTTCTATCTTTTCCACTTGTTTTCCAGTA 13 0.325 No Hit CATTCTTCGGCCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTA 12 0.3 No Hit CGCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGGCGCAACAC 12 0.3 No Hit ACCCTTCCACATTGATAATCCTTGAATTGCTGGATGTCTCGCGCTAGCTT 12 0.3 No Hit AGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAATGGCT 12 0.3 No Hit GCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACAAATAGA 11 0.27499999999999997 No Hit AGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACAAATAG 11 0.27499999999999997 No Hit GCCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACAAA 11 0.27499999999999997 No Hit GACAGACGTTAACAAACAAATAGAAAAAACGATACACGGCGCAGTCAAAA 10 0.25 No Hit GCCATCTTATAAGAATCTCACTTGATTGCTCGACTACATATGAACTGCAC 9 0.22499999999999998 No Hit ATTGCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGGCGCAAC 9 0.22499999999999998 No Hit CTCACGCCCGCGCGCAGGCGCAACACACACAAAACATCGAAAAAACGTTA 9 0.22499999999999998 No Hit GCACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACA 9 0.22499999999999998 No Hit GGTCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACAA 9 0.22499999999999998 No Hit CTAGTGTTGTACCATCCAAATACTGTTCTGCTCCTGCAATTTCAGTGTCT 8 0.2 No Hit CCACACTTGTTTCAGAGGATTTTTCTAGTGTTGTACCATCCAAATACTGT 8 0.2 No Hit GTCCGTGTCAGATAGCTCCTTTGGAGAAGCTTCCACACTTGTTTCAGAGG 8 0.2 No Hit CAATATTCTACAGTTCAGTTCTACATTCTTCGGCCAGCAAACTTGATCGC 8 0.2 No Hit CTTCCACATTGATAATCCTTGAATTGCTGGATGTCTCGCGCTAGCTTGCT 8 0.2 No Hit CAGTTCAGTTCTACATTCTTCGGCCAGCAAACTTGATCGCCATAATCTCA 8 0.2 No Hit GAGGATTTTTCTAGTGTTGTACCATCCAAATACTGTTCTGCTCCTGCAAT 8 0.2 No Hit GGGACAGACGTTAACAAACAAATAGAAAAAACGATACACGGCGCAGTCAA 8 0.2 No Hit GCAGGCGCAACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAG 7 0.17500000000000002 No Hit CATAATCTCAGGGACAGACGTTAACAAACAAATAGAAAAAACGATACACG 7 0.17500000000000002 No Hit AGGCGCAACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTA 7 0.17500000000000002 No Hit AACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACAC 7 0.17500000000000002 No Hit CCCAGAACTGCTGTCCGTGTCAGATAGCTCCTTTGGAGAAGCTTCCACAC 7 0.17500000000000002 No Hit CACACTTGTTTCAGAGGATTTTTCTAGTGTTGTACCATCCAAATACTGTT 7 0.17500000000000002 No Hit ATCCAAATACTGTTCTGCTCCTGCAATTTCAGTGTCTTCATGAATATCAT 7 0.17500000000000002 No Hit TCGAAAAAACGTTACAACACCAAGTAGTACAACACATACACAGCAACAAC 7 0.17500000000000002 No Hit CATCCAAATACTGTTCTGCTCCTGCAATTTCAGTGTCTTCATGAATATCA 7 0.17500000000000002 No Hit CGCCATAATCTCAGGGACAGACGTTAACAAACAAATAGAAAAAACGATAC 7 0.17500000000000002 No Hit TCTTATAAGAATCTCACTTGATTGCTCGACTACATATGAACTGCACTAGA 6 0.15 No Hit AACAGTTCGAGAGTTCAGCTCAATTGTTCTATCTTTTCCACTTGTTTTCC 6 0.15 No Hit GCCCGCGCGCAGGCGCAACACACACAAAACATCGAAAAAACGTTACAACA 6 0.15 No Hit CCATAATCTCAGGGACAGACGTTAACAAACAAATAGAAAAAACGATACAC 6 0.15 No Hit CGGCCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAACA 6 0.15 No Hit TCTACATTCTTCGGCCAGCAAACTTGATCGCCATAATCTCAGGGACAGAC 6 0.15 No Hit AGGGACAGACGTTAACAAACAAATAGAAAAAACGATACACGGCGCAGTCA 6 0.15 No Hit TGAATTGCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGGCGC 6 0.15 No Hit CCTCAGGGACAGACGTTAACAAACAAATAGAAAAAACGATACACGGCGCA 5 0.125 No Hit CCGTCTTATAAGAATCTCACTTGATTGCTCGACTACATATGAACTGCACT 5 0.125 No Hit AACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTCAACCAA 5 0.125 No Hit GATCGCCATAATCTCAGGGACAGACGTTAACAAACAAATAGAAAAAACGA 5 0.125 No Hit TAGTGTTGTACCATCCAAATACTGTTCTGCTCCTGCAATTTCAGTGTCTT 5 0.125 No Hit GTTAACAAACAAATAGAAAAAACGATACACGGCGCAGTCAAAACTTTGAT 5 0.125 No Hit GCACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACACATA 5 0.125 No Hit CCCCTCCACATTGATAATCCTTGAATTGCTGGATGTCTCGCGCTAGCTTG 5 0.125 No Hit CAACACACACAAAACATCGAAAAAACGTTACAACACCAAGTAGTACAACA 5 0.125 No Hit GGATTTTTCTAGTGTTGTACCATCCAAATACTGTTCTGCTCCTGCAATTT 5 0.125 No Hit CAGTTCTACATTCTTCGGCCAGCAAACTTGATCGCCATAATCTCAGGGAC 5 0.125 No Hit CGGCGCAGTCAAAACTTTGATGATTCCCTGTGCCTTCTCACTCAAAATCC 5 0.125 No Hit CGGCTCAATTGTTCTATCTTTTCCACTTGTTTTCCAGTAACCATTGGGTG 5 0.125 No Hit CAACCCAACAGTTCGAGAGTTCAGCTCAATTGTTCTATCTTTTCCACTTG 5 0.125 No Hit CACGCCCGCGCGCAGGCGCAACACACACAAAACATCGAAAAAACGTTACA 5 0.125 No Hit GCTGTCCGTGTCAGATAGCTCCTTTGGAGAAGCTTCCACACTTGTTTCAG 5 0.125 No Hit TTACAACACCAAGTAGTACAACACATACACAGCAACAACACCGGCTGCTC 5 0.125 No Hit TTGAATTGCTGGATGTCTCGCGCTAGCTTGCTCACGCCCGCGCGCAGGCG 5 0.125 No Hit CTCGGCCAGCAAACTTGATCGCCATAATCTCAGGGACAGACGTTAACAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCCTT 10 0.0070870025 143.20253 1 CCATCTT 20 1.9964991E-6 143.20253 1 CATCTTA 25 6.059723E-6 114.562035 2 ATCTTAT 35 3.4368895E-5 80.807144 3 TCTTATA 35 3.4368895E-5 80.807144 4 TATAAGA 40 6.6638866E-5 70.706245 7 TTATAAG 40 6.6638866E-5 70.706245 6 CTTATAA 40 6.6638866E-5 70.706245 5 ATAAGAA 50 2.0113261E-4 56.565 8 TAAGAAT 50 2.0113261E-4 56.565 9 TGCTGGA 55 3.2214308E-4 51.422726 8 ATTGCTG 55 3.2214308E-4 51.422726 6 GAATTGC 55 3.2214308E-4 51.422726 4 TGAATTG 55 3.2214308E-4 51.422726 3 AATTGCT 55 3.2214308E-4 51.422726 5 TTGCTGG 55 3.2214308E-4 51.422726 7 CTTGAAT 45 0.009401072 47.734177 1 GCTGGAT 70 0.0010581203 40.403572 9 AGAATCT 40 0.008844503 35.353123 9 TTATCCC 25 2.0234297E-4 33.77015 140-144 >>END_MODULE ERR5262803 read2 length is 65-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262803_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 65-150 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0775 37.0 37.0 37.0 37.0 37.0 2 35.9835 37.0 37.0 37.0 37.0 37.0 3 36.0235 37.0 37.0 37.0 37.0 37.0 4 36.017 37.0 37.0 37.0 37.0 37.0 5 36.0375 37.0 37.0 37.0 37.0 37.0 6 36.052 37.0 37.0 37.0 37.0 37.0 7 36.184 37.0 37.0 37.0 37.0 37.0 8 36.2 37.0 37.0 37.0 37.0 37.0 9 36.2375 37.0 37.0 37.0 37.0 37.0 10-14 36.2188 37.0 37.0 37.0 37.0 37.0 15-19 36.24640000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.125800000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.1342 37.0 37.0 37.0 37.0 37.0 30-34 36.1274 37.0 37.0 37.0 37.0 37.0 35-39 36.1019 37.0 37.0 37.0 37.0 37.0 40-44 36.1361 37.0 37.0 37.0 37.0 37.0 45-49 36.1534 37.0 37.0 37.0 37.0 37.0 50-54 36.158 37.0 37.0 37.0 37.0 37.0 55-59 35.99040000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.0413 37.0 37.0 37.0 37.0 37.0 65-69 36.05951477869468 37.0 37.0 37.0 37.0 37.0 70-74 36.02026016887008 37.0 37.0 37.0 37.0 37.0 75-79 35.948403018980954 37.0 37.0 37.0 37.0 37.0 80-84 35.87153380595956 37.0 37.0 37.0 37.0 37.0 85-89 35.90474583457901 37.0 37.0 37.0 37.0 37.0 90-94 35.85987298736814 37.0 37.0 37.0 37.0 37.0 95-99 35.78637580667324 37.0 37.0 37.0 37.0 37.0 100-104 35.669897473522475 37.0 37.0 37.0 37.0 37.0 105-109 35.68623199237062 37.0 37.0 37.0 37.0 37.0 110-114 35.7243701597169 37.0 37.0 37.0 37.0 37.0 115-119 35.70892112933277 37.0 37.0 37.0 37.0 37.0 120-124 35.57946624712237 37.0 37.0 37.0 37.0 37.0 125-129 35.64675689049261 37.0 37.0 37.0 37.0 37.0 130-134 35.46552960419527 37.0 37.0 37.0 37.0 37.0 135-139 35.61782743642126 37.0 37.0 37.0 37.0 37.0 140-144 35.502571162095414 37.0 37.0 37.0 37.0 37.0 145-149 35.42610897204606 37.0 37.0 37.0 37.0 37.0 150 35.37120291616039 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 0.0 18 1.0 19 1.0 20 5.0 21 9.0 22 9.0 23 10.0 24 9.0 25 4.0 26 7.0 27 16.0 28 10.0 29 11.0 30 22.0 31 20.0 32 58.0 33 94.0 34 201.0 35 528.0 36 2748.0 37 235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.474999999999994 17.675 4.55 24.3 2 37.25 19.15 21.775 21.825 3 28.075 22.7 23.625 25.6 4 29.7 32.175 15.2 22.925 5 28.249999999999996 34.775 14.875 22.1 6 28.1 35.875 12.3 23.724999999999998 7 25.3 21.05 25.3 28.349999999999998 8 25.15 19.7 18.575 36.575 9 29.15 22.025 19.875 28.95 10-14 32.910000000000004 23.885 17.28 25.924999999999997 15-19 31.255 22.39 19.82 26.534999999999997 20-24 29.955 22.68 21.505 25.86 25-29 29.015 21.825 22.95 26.21 30-34 33.305 21.47 20.380000000000003 24.845 35-39 30.925000000000004 21.93 21.39 25.755 40-44 30.89 21.375 22.735 25.0 45-49 32.205 22.79 20.255000000000003 24.75 50-54 30.695 23.77 20.28 25.255 55-59 30.964999999999996 22.515 21.68 24.84 60-64 29.79 21.7 23.119999999999997 25.39 65-69 29.185837167433487 23.179635927185437 23.309661932386476 24.324864972994597 70-74 28.9238004703057 21.609045879821885 24.691049181968282 24.776104467904137 75-79 31.466746734724516 18.8109893409398 25.231446729720265 24.49081719461542 80-84 28.122808774917356 20.55494340378644 25.999198637684064 25.323049183612138 85-89 29.589123563939197 19.219384939547485 26.87503135503938 24.31646014147394 90-94 28.395434203248353 18.323527932820436 29.391059486096445 23.889978377834765 95-99 28.833240773463924 19.644570101479275 27.62659665774726 23.895592467309537 100-104 27.65159196917461 19.77793551003853 29.050902453863316 23.519570066923546 105-109 30.356413398692812 20.85375816993464 26.78717320261438 22.00265522875817 110-114 28.744584278935427 20.105219723540337 25.763358778625957 25.38683721889829 115-119 30.255822435262857 18.465065388422865 24.71213463241807 26.566977543896215 120-124 28.64933552178747 20.606766559008843 26.62678032509133 24.117117594112354 125-129 30.49854947888686 21.564413882024283 25.443214784570756 22.493821854518103 130-134 31.322327647671806 21.50496352766961 24.78473098228487 22.38797784237372 135-139 31.204554421960427 19.98759934614734 24.378558142156585 24.42928808973564 140-144 29.234471693788677 21.286958514783404 25.739170295668117 23.7393994957598 145-149 29.492706549341523 19.76023150062009 26.663910706903682 24.083151243134708 150 33.44471445929526 20.018226002430133 26.032806804374243 20.504252733900362 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.0 24 0.5 25 1.0 26 0.5 27 0.5 28 0.5 29 0.0 30 0.0 31 4.0 32 9.0 33 7.5 34 4.5 35 2.5 36 4.5 37 19.5 38 21.0 39 16.0 40 59.0 41 93.0 42 169.5 43 190.0 44 95.5 45 83.5 46 120.0 47 123.0 48 89.5 49 74.5 50 100.5 51 124.0 52 104.0 53 67.5 54 70.5 55 78.5 56 77.0 57 81.5 58 99.0 59 188.5 60 307.5 61 328.5 62 191.0 63 87.0 64 102.5 65 85.0 66 77.0 67 67.0 68 56.5 69 85.5 70 107.5 71 124.5 72 103.5 73 66.5 74 30.5 75 2.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 64-65 1.0 66-67 0.0 68-69 0.0 70-71 2.0 72-73 0.0 74-75 0.0 76-77 1.0 78-79 1.0 80-81 1.0 82-83 4.0 84-85 1.0 86-87 3.0 88-89 4.0 90-91 4.0 92-93 7.0 94-95 8.0 96-97 3.0 98-99 10.0 100-101 2.0 102-103 13.0 104-105 2.0 106-107 28.0 108-109 14.0 110-111 10.0 112-113 17.0 114-115 14.0 116-117 23.0 118-119 28.0 120-121 24.0 122-123 18.0 124-125 28.0 126-127 14.0 128-129 27.0 130-131 46.0 132-133 32.0 134-135 50.0 136-137 25.0 138-139 21.0 140-141 23.0 142-143 32.0 144-145 43.0 146-147 51.0 148-149 73.0 150-151 3292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.775 #Duplication Level Percentage of deduplicated Percentage of total 1 46.75072744907857 12.049999999999999 2 14.936954413191076 7.7 3 10.184287099903006 7.875 4 6.110572259941804 6.3 5 5.431619786614937 7.000000000000001 6 2.909796314258002 4.5 7 2.0368574199806013 3.675 8 1.3579049466537343 2.8000000000000003 9 1.939864209505335 4.5 >10 7.953443258971872 36.85 >50 0.38797284190106696 6.75 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTGAACACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGG 82 2.0500000000000003 No Hit GGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCT 67 1.675 No Hit GGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTA 66 1.6500000000000001 No Hit GTGAACACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGC 55 1.375 No Hit GCCACATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGGATTCTGGGA 50 1.25 No Hit GGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGAT 49 1.225 No Hit TGAACACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCA 46 1.15 No Hit GGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGA 45 1.125 No Hit CAGGAGGTGAACACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGA 44 1.0999999999999999 No Hit GCCGAACGGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCC 41 1.0250000000000001 No Hit GGCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAACGG 35 0.8750000000000001 No Hit GAACACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCAC 30 0.75 No Hit GCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGGATT 29 0.7250000000000001 No Hit GACGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAAA 29 0.7250000000000001 No Hit AGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGT 28 0.7000000000000001 No Hit CTCAGAACTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTT 28 0.7000000000000001 No Hit CATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGGATTCTGGGATAAC 26 0.65 No Hit CGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGT 25 0.625 No Hit GCGCAGTGGAAAGCCTCCAACGACCCCTCCGGCCGGCTCATCGCCTCGCA 24 0.6 No Hit GTTGGCCATACCAGCTGCAAAGGCATCGTTAACGGGGTGGTGACGGCCAA 24 0.6 No Hit GCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGATATGGCTAGTAGTTCTG 24 0.6 No Hit AGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCACAGGGAATCATC 24 0.6 No Hit GCCAGCCGTAGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAA 22 0.5499999999999999 No Hit GGCCACATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGGATTCTGGG 22 0.5499999999999999 No Hit GTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGATATGGCTAGTAGTTC 21 0.525 No Hit GTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCACAGGGAATCATCA 21 0.525 No Hit GGCCAGCCGTAGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGA 21 0.525 No Hit CAGAACTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGC 20 0.5 No Hit GTTCAACAGGAGGTGAACACTGAGCAGTACATCCATTTTTGCGGGATTTT 19 0.475 No Hit GTGACGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAA 18 0.44999999999999996 No Hit CCTCAGAACTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGT 18 0.44999999999999996 No Hit AGGAGGTGAACACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAG 17 0.42500000000000004 No Hit GGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAG 17 0.42500000000000004 No Hit GCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTGCAAGCCATT 17 0.42500000000000004 No Hit CATTGGTTGAGCAGCCGGTGTTGTTGCTGTGTATGTGTTGTACTACTTGG 16 0.4 No Hit CTTGGAGCAAGCTAGAGTCAGAGCCCCGCAAGCACTTAATTAGTCTCTTC 16 0.4 No Hit GATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGCTGTGTAT 16 0.4 No Hit GTCAGAGCCCCGCAAGCACTTAATTAGTCTCTTCATTCGTTTTGCTATTC 16 0.4 No Hit CAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCG 16 0.4 No Hit GTGCGTGTCCTCAGAACTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCC 15 0.375 No Hit GGGAGGTTCAACAGGAGGTGAACACTGAGCAGTACATCCATTTTTGCGGG 15 0.375 No Hit CTTCGAGAGGCTCTACCCTCAGGACTCGGCGAGGGAGACCGTGCCCTGGG 15 0.375 No Hit AGCACGCCGGCGGCAATCGAGTGGGTGGCGCAGTGGAAAGCCTCCAACGA 15 0.375 No Hit GAAGAGTACCCATACAACACGCCTATCAACAAGGAGGCGTACTACTACCG 14 0.35000000000000003 No Hit ACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGATATGGC 14 0.35000000000000003 No Hit CGGCAATCGAGTGGGTGGCGCAGTGGAAAGCCTCCAACGACCCCTCCGGC 14 0.35000000000000003 No Hit AAGGCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAAC 14 0.35000000000000003 No Hit GCACGCCGGCGGCAATCGAGTGGGTGGCGCAGTGGAAAGCCTCCAACGAC 14 0.35000000000000003 No Hit CGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAAACC 13 0.325 No Hit CCTGCGGCTAGGCCAGCCGTAGCAGAGGATATGGCTAGTAGTTCTGTGCG 13 0.325 No Hit CACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCACAGG 13 0.325 No Hit GGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGGAT 13 0.325 No Hit ACCAGCTGCAAAGGCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGG 13 0.325 No Hit AACGGAGTGGTGCCGAACGGGAAAACCAACGGGATTCTGGGATAACCCGG 12 0.3 No Hit CCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGATATGGCT 12 0.3 No Hit GTGGAAAGCCTCCAACGACCCCTCCGGCCGGCTCATCGCCTCGCACAACG 12 0.3 No Hit GGTGCCGAACGGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCT 12 0.3 No Hit GGCTCATCGCCTCGCACAACGACTCCTCCGCTGCCGGCCCCGCCGCTCAC 12 0.3 No Hit GTGCCGAACGGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTC 12 0.3 No Hit AGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCC 12 0.3 No Hit CCTCCAACGACCCCTCCGGCCGGCTCATCGCCTCGCACAACGACTCCTCC 12 0.3 No Hit GGAGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGGCGGCAA 12 0.3 No Hit CTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAG 12 0.3 No Hit GGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTGCAAGCCAT 12 0.3 No Hit CGAGCATCGCGTGCAGCACGCCGGCGGCAATCGAGTGGGTGGCGCAGTGG 11 0.27499999999999997 No Hit GAACGGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGC 11 0.27499999999999997 No Hit GTGGTGCCGAACGGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTG 11 0.27499999999999997 No Hit CTCCAACGACCCCTCCGGCCGGCTCATCGCCTCGCACAACGACTCCTCCG 11 0.27499999999999997 No Hit ATCGAGTGGGTGGCGCAGTGGAAAGCCTCCAACGACCCCTCCGGCCGGCT 11 0.27499999999999997 No Hit CATCGCCTCGCACAACGACTCCTCCGCTGCCGGCCCCGCCGCTCACACCG 11 0.27499999999999997 No Hit GTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAACGGAGTGGT 11 0.27499999999999997 No Hit GGGGTGGTGACGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAA 11 0.27499999999999997 No Hit GCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAACGGA 11 0.27499999999999997 No Hit GTTTAGTGACGGTGTGGGGTACAACTGGATAGACGGCCTGAAGGCCTTCA 11 0.27499999999999997 No Hit GTCGGCTCTGGCACTGGCTCTGGAGGAGGACAAACCAGCACCGGTTCAGG 11 0.27499999999999997 No Hit GGAGGTGAACACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGA 11 0.27499999999999997 No Hit GTACAACTGGATAGACGGCCTGAAGGCCTTCACGGAGCAGCAGGTCACCG 11 0.27499999999999997 No Hit ACTTTGTGTTCATGCTATATCAGAACTTGAGCTGTACAAATTTGCTCCCT 10 0.25 No Hit GTCCTCAGAACTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTT 10 0.25 No Hit GGGTAATGGTAGCGGCACCGGCGGCGGCGGCGGTAGCAGCTCGAACGGTG 10 0.25 No Hit GATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGATA 10 0.25 No Hit AGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTGCAAGCCATTGGT 10 0.25 No Hit GCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAAACCAA 10 0.25 No Hit AACGACTCCTCCGCTGCCGGCCCCGCCGCTCACACCGAGGACGTTGGCCA 10 0.25 No Hit AGGAGGACAAACCAGCACCGGTTCAGGCTCTGCCACGGGTAATGGTAGCG 10 0.25 No Hit GCGAGGGAGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGGC 10 0.25 No Hit ATCGCGTGCAGCACGCCGGCGGCAATCGAGTGGGTGGCGCAGTGGAAAGC 9 0.22499999999999998 No Hit GCTAGGCCAGCCGTAGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCT 9 0.22499999999999998 No Hit AAAGCCTCCAACGACCCCTCCGGCCGGCTCATCGCCTCGCACAACGACTC 9 0.22499999999999998 No Hit CGAGGACGTTGGCCATACCAGCTGCAAAGGCATCGTTAACGGGGTGGTGA 9 0.22499999999999998 No Hit GCCGTAGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGA 9 0.22499999999999998 No Hit GAGTGGGTGGCGCAGTGGAAAGCCTCCAACGACCCCTCCGGCCGGCTCAT 9 0.22499999999999998 No Hit GCCTTACCTGCCGAAGCATATCCTCTACCGCCAGAAGGAGCAGTTTAGTG 9 0.22499999999999998 No Hit CGCCGCTCACACCGAGGACGTTGGCCATACCAGCTGCAAAGGCATCGTTA 9 0.22499999999999998 No Hit CCGAACGGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCT 9 0.22499999999999998 No Hit GCCGGCCCCGCCGCTCACACCGAGGACGTTGGCCATACCAGCTGCAAAGG 9 0.22499999999999998 No Hit GACTCGGCGAGGGAGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCAC 9 0.22499999999999998 No Hit CGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGGA 9 0.22499999999999998 No Hit GTTGGAACTTGGAGCAAGCTAGAGTCAGAGCCCCGCAAGCACTTAATTAG 9 0.22499999999999998 No Hit GGCCATACCAGCTGCAAAGGCATCGTTAACGGGGTGGTGACGGCCAACGG 9 0.22499999999999998 No Hit CTCACACCGAGGACGTTGGCCATACCAGCTGCAAAGGCATCGTTAACGGG 9 0.22499999999999998 No Hit GAACTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGCTG 9 0.22499999999999998 No Hit GAGATGATGAAAAACGCCGCAGAAGAGTACCCATACAACACGCCTATCAA 9 0.22499999999999998 No Hit GCAATCGAGTGGGTGGCGCAGTGGAAAGCCTCCAACGACCCCTCCGGCCG 9 0.22499999999999998 No Hit CAACGGAGTGGTGCCGAACGGGAAAACCAACGGGATTCTGGGATAACCCG 9 0.22499999999999998 No Hit ACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCG 9 0.22499999999999998 No Hit ATTTGCTCCCTGGGACCTTCCTGAAAAATCCTGTCTTCGAAGCAAAGATC 8 0.2 No Hit GGTTCAACAGGAGGTGAACACTGAGCAGTACATCCATTTTTGCGGGATTT 8 0.2 No Hit GCCATTGGTTGAGCAGCCGGTGTTGTTGCTGTGTATGTGTTGTACTACTT 8 0.2 No Hit CCGAGGACGTTGGCCATACCAGCTGCAAAGGCATCGTTAACGGGGTGGTG 8 0.2 No Hit GGCAGCGGTTCCGGTACCGGTAACGGTGGGTTCCATTGAAACCCCATGGT 8 0.2 No Hit CTCAGGACTCGGCGAGGGAGACCGTGCCCTGGGGCCCGAGCATCGCGTGC 8 0.2 No Hit GGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGCTGTGTATGTG 8 0.2 No Hit GTAGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGG 8 0.2 No Hit GTGGTGACGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGG 8 0.2 No Hit CGAACGGGAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTG 8 0.2 No Hit GCACACACTACGGTGATGGCTCAAACTTGCCTGCCACCTGGCTTTCGTTT 8 0.2 No Hit GCAGTGGAAAGCCTCCAACGACCCCTCCGGCCGGCTCATCGCCTCGCACA 8 0.2 No Hit AGGACGTTGGCCATACCAGCTGCAAAGGCATCGTTAACGGGGTGGTGACG 8 0.2 No Hit ACTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCACAGGG 8 0.2 No Hit CCACATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGGATTCTGGGAT 7 0.17500000000000002 No Hit GGCGAGGGAGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGG 7 0.17500000000000002 No Hit CTCTACCCTCAGGACTCGGCGAGGGAGACCGTGCCCTGGGGCCCGAGCAT 7 0.17500000000000002 No Hit CCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGATATGGCTA 7 0.17500000000000002 No Hit AGTGGGTGGCGCAGTGGAAAGCCTCCAACGACCCCTCCGGCCGGCTCATC 7 0.17500000000000002 No Hit AGTACCCATACAACACGCCTATCAACAAGGAGGCGTACTACTACCGAATG 7 0.17500000000000002 No Hit GGCTCTGCCAGTGGATCGGGTAGCGGCGAGGGTGGTGGTACAGCTGCAGG 7 0.17500000000000002 No Hit CAGCGGTTCCGGTACCGGTAACGGTGGGTTCCATTGAAACCCCATGGTTG 7 0.17500000000000002 No Hit CTACCCTCAGGACTCGGCGAGGGAGACCGTGCCCTGGGGCCCGAGCATCG 7 0.17500000000000002 No Hit GCCGCTCACACCGAGGACGTTGGCCATACCAGCTGCAAAGGCATCGTTAA 7 0.17500000000000002 No Hit GACCCCTCCGGCCGGCTCATCGCCTCGCACAACGACTCCTCCGCTGCCGG 7 0.17500000000000002 No Hit ACTGGATAGACGGCCTGAAGGCCTTCACGGAGCAGCAGGTCACCGATGAG 7 0.17500000000000002 No Hit GTGGGTCTGGCAGCGGTGGAGGCAGCGGTTCCGGTACCGGTAACGGTGGG 7 0.17500000000000002 No Hit GTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTGCAAGCCATTGGTTGAG 7 0.17500000000000002 No Hit GGTGCTCCCTGCGGCTAGGCCAGCCGTAGCAGAGGATATGGCTAGTAGTT 7 0.17500000000000002 No Hit CAGCTGCAAAGGCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCC 7 0.17500000000000002 No Hit CTTTGTGTTCATGCTATATCAGAACTTGAGCTGTACAAATTTGCTCCCTG 7 0.17500000000000002 No Hit AAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGC 7 0.17500000000000002 No Hit CTCGCACAACGACTCCTCCGCTGCCGGCCCCGCCGCTCACACCGAGGACG 7 0.17500000000000002 No Hit ACGACCCCTCCGGCCGGCTCATCGCCTCGCACAACGACTCCTCCGCTGCC 7 0.17500000000000002 No Hit GCCATACCAGCTGCAAAGGCATCGTTAACGGGGTGGTGACGGCCAACGGC 7 0.17500000000000002 No Hit GGTGGAGGCAGCGGTTCCGGTACCGGTAACGGTGGGTTCCATTGAAACCC 6 0.15 No Hit AGAACTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGCT 6 0.15 No Hit GCTATATCAGAACTTGAGCTGTACAAATTTGCTCCCTGGGACCTTCCTGA 6 0.15 No Hit GGTGACGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGA 6 0.15 No Hit TGACGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAA 6 0.15 No Hit AGCATATCCTCTACCGCCAGAAGGAGCAGTTTAGTGACGGTGTGGGGTAC 6 0.15 No Hit GTCTCATTCTGCCTTCCCGGAGGTGCACAGGTTAATGCTGTTGAACGGAC 6 0.15 No Hit GTAACGGTGGGTTCCATTGAAACCCCATGGTTGGAACTTGGAGCAAGCTA 6 0.15 No Hit AACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCC 6 0.15 No Hit CTGAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCACAGGGA 6 0.15 No Hit CTGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGCTGTGT 6 0.15 No Hit CCTGAAGGCCTTCACGGAGCAGCAGGTCACCGATGAGATGATGAAAAACG 6 0.15 No Hit ACTTAATTAGTCTCTTCATTCGTTTTGCTATTCCAGATCAATTTAGTTTC 6 0.15 No Hit AACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAACGGAGTGGTGCC 6 0.15 No Hit AGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCACAGGGAATC 6 0.15 No Hit GGGGCGGCGGCGGGAGGTTCAACAGGAGGTGAACACTGAGCAGTACATCC 6 0.15 No Hit CTTTCGTTTCCATCCAACAGATGTCGAGCTCGTTTCATACTACCTAAAAA 6 0.15 No Hit TGATGGCCCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGCTGTGTA 6 0.15 No Hit AATTAGTCTCTTCATTCGTTTTGCTATTCCAGATCAATTTAGTTTCCTTT 6 0.15 No Hit CGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGGCGGCAATCGAGT 6 0.15 No Hit CAGTGGAAAGCCTCCAACGACCCCTCCGGCCGGCTCATCGCCTCGCACAA 6 0.15 No Hit GCAGAGTATTCTTATGGAAGAACGGCTTGAATGGCTGACGGATGGTGTTA 6 0.15 No Hit CCATACAACACGCCTATCAACAAGGAGGCGTACTACTACCGAATGATCTT 6 0.15 No Hit CTCCTCCGCTGCCGGCCCCGCCGCTCACACCGAGGACGTTGGCCATACCA 6 0.15 No Hit GCTTGAATGGCTGACGGATGGTGTTAGCATGCTAACTTCGTTATCACCGG 6 0.15 No Hit GGGAGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGGCGGCA 6 0.15 No Hit AGTAACCACACCCCGGTGTTATTGCATCCTGTCCAGGCTCCCATTCTTTG 6 0.15 No Hit GGATTTTGAGTGAGAAGGCACAGGGAATCATCAAAGTTTTGACTGCGCCG 6 0.15 No Hit TAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTGCAAGCCATTGG 6 0.15 No Hit AGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGGCGGCAATC 6 0.15 No Hit GTCGAGCTCGTTTCATACTACCTAAAAAGGAAGATTATGGGGAAGAAACT 5 0.125 No Hit ATTTTTGCGGGATTTTGAGTGAGAAGGCACAGGGAATCATCAAAGTTTTG 5 0.125 No Hit CGGTGCTCCCAGCGTTGGTGGCGGTTCTGGCTTTGGGGCTGGACAGGCTG 5 0.125 No Hit AATTTGCTCCCTGGGACCTTCCTGAAAAATCCTGTCTTCGAAGCAAAGAT 5 0.125 No Hit GTTTCATACTACCTAAAAAGGAAGATTATGGGGAAGAAACTTTGTGTTCA 5 0.125 No Hit GGTTGAGCAGCCGGTGTTGTTGCTGTGTATGTGTTGTACTACTTGGTGTT 5 0.125 No Hit GAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTG 5 0.125 No Hit GGCCTGAAGGCCTTCACGGAGCAGCAGGTCACCGATGAGATGATGAAAAA 5 0.125 No Hit GGTGGTACAGCTGCAGGTGATGCTAATGCACCAAGTGTTGGAGTCGGCTC 5 0.125 No Hit CTAGAGTCAGAGCCCCGCAAGCACTTAATTAGTCTCTTCATTCGTTTTGC 5 0.125 No Hit GCATCGCGTGCAGCACGCCGGCGGCAATCGAGTGGGTGGCGCAGTGGAAA 5 0.125 No Hit AAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGG 5 0.125 No Hit GTCATGATGGCCAAGTGAAGGTCCCGTGAAATAAGATTGAAATTATCAGA 5 0.125 No Hit GGAGAAGTGGGTATTGAGGAAGGCCTTCGATGTCGAGGAGGAGCCTTACC 5 0.125 No Hit ATCGCCTCGCACAACGACTCCTCCGCTGCCGGCCCCGCCGCTCACACCGA 5 0.125 No Hit AGGACAAACCAGCACCGGTTCAGGCTCTGCCACGGGTAATGGTAGCGGCA 5 0.125 No Hit GGCTAGGCCAGCCGTAGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCC 5 0.125 No Hit GAGGGAGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGGCGG 5 0.125 No Hit GCTCGTTTCATACTACCTAAAAAGGAAGATTATGGGGAAGAAACTTTGTG 5 0.125 No Hit GGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTGCA 5 0.125 No Hit GAGACCGTGCCCTGGGGCCCGAGCATCGCGTGCAGCACGCCGGCGGCAAT 5 0.125 No Hit AGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAGAACTGATGGCCCGTGC 5 0.125 No Hit GAGGACGTTGGCCATACCAGCTGCAAAGGCATCGTTAACGGGGTGGTGAC 5 0.125 No Hit CGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAACGGAGTGG 5 0.125 No Hit CGGGAGGTTCAACAGGAGGTGAACACTGAGCAGTACATCCATTTTTGCGG 5 0.125 No Hit GTACTACTACCGAATGATCTTCGAGAGGCTCTACCCTCAGGACTCGGCGA 5 0.125 No Hit ACGGCAACGGCCACATCAACGGAGTGGTGCCGAACGGGAAAACCAACGGG 5 0.125 No Hit CTTTGAACTGCATTTTGGGGTACTGCAGAGTATTCTTATGGAAGAACGGC 5 0.125 No Hit CCGTGCAAGCCATTGGTTGAGCAGCCGGTGTTGTTGCTGTGTATGTGTTG 5 0.125 No Hit GGTAGCAGCTCGAACGGTGGGTCTGGCAGCGGTGGAGGCAGCGGTTCCGG 5 0.125 No Hit GGAGGTTCAACAGGAGGTGAACACTGAGCAGTACATCCATTTTTGCGGGA 5 0.125 No Hit GCTGCAAAGGCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCAC 5 0.125 No Hit GTTCCATTGAAACCCCATGGTTGGAACTTGGAGCAAGCTAGAGTCAGAGC 5 0.125 No Hit CTTCACGGAGCAGCAGGTCACCGATGAGATGATGAAAAACGCCGCAGAAG 5 0.125 No Hit GACTCCTCCGCTGCCGGCCCCGCCGCTCACACCGAGGACGTTGGCCATAC 5 0.125 No Hit GAGCAGTACATCCATTTTTGCGGGATTTTGAGTGAGAAGGCACAGGGAAT 5 0.125 No Hit GTTGATTTGTGGCCCTGAGTTCTCATATCAGGACTGACCAACATCCCCTT 5 0.125 No Hit GGTGGCGCAGTGGAAAGCCTCCAACGACCCCTCCGGCCGGCTCATCGCCT 5 0.125 No Hit AGGCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAACG 5 0.125 No Hit GATGAAAAACGCCGCAGAAGAGTACCCATACAACACGCCTATCAACAAGG 5 0.125 No Hit AGATGATGAAAAACGCCGCAGAAGAGTACCCATACAACACGCCTATCAAC 5 0.125 No Hit ATACAACACGCCTATCAACAAGGAGGCGTACTACTACCGAATGATCTTCG 5 0.125 No Hit CATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATCAACGGAG 5 0.125 No Hit AGGCCAGCCGTAGCAGAGGATATGGCTAGTAGTTCTGTGCGTGTCCTCAG 5 0.125 No Hit TGAGCAGCCGGTGTTGTTGCTGTGTATGTGTTGTACTACTTGGTGTTGTA 5 0.125 No Hit GGCACACACTACGGTGATGGCTCAAACTTGCCTGCCACCTGGCTTTCGTT 5 0.125 No Hit GGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTA 5 0.125 No Hit ATTGAAACCCCATGGTTGGAACTTGGAGCAAGCTAGAGTCAGAGCCCCGC 5 0.125 No Hit GCTCGAACGGTGGGTCTGGCAGCGGTGGAGGCAGCGGTTCCGGTACCGGT 5 0.125 No Hit GGCCCCGCCGCTCACACCGAGGACGTTGGCCATACCAGCTGCAAAGGCAT 5 0.125 No Hit GATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAGGCCAGCCGTAGC 5 0.125 No Hit ATGAAATTCTCCTGGGGCAGGAACAGCTTAAAGAAAGCAATCAGTCTTTC 5 0.125 No Hit GAAAACCAACGGGATTCTGGGATAACCCGGTGGGTGCTCCCTGCGGCTAG 5 0.125 No Hit GCAAAGGCATCGTTAACGGGGTGGTGACGGCCAACGGCAACGGCCACATC 5 0.125 No Hit CATGGTTGGAACTTGGAGCAAGCTAGAGTCAGAGCCCCGCAAGCACTTAA 5 0.125 No Hit GGGGAAGAAACTTTGTGTTCATGCTATATCAGAACTTGAGCTGTACAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCGAT 25 0.0055328575 29.376621 120-124 TGTGTGT 40 0.004959782 19.526934 130-134 GTTGTAC 70 0.005825323 13.928572 135-139 ACTACTT 75 0.006453254 13.709091 140-144 >>END_MODULE Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550655 spots for ERR5262803.sra Written 1550655 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra Read 1550647 spots for ERR5262803.sra Written 1550647 spots for ERR5262803.sra SRR ids: ['ERR5262803.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xj2z22z8 ERR5262803.sra spots: 31012948 blocks: [[1, 1550647], [1550648, 3101294], [3101295, 4651941], [4651942, 6202588], [6202589, 7753235], [7753236, 9303882], [9303883, 10854529], [10854530, 12405176], [12405177, 13955823], [13955824, 15506470], [15506471, 17057117], [17057118, 18607764], [18607765, 20158411], [20158412, 21709058], [21709059, 23259705], [23259706, 24810352], [24810353, 26360999], [26361000, 27911646], [27911647, 29462293], [29462294, 31012948]] ERR5262803 file size 10150006 ERR5262803 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262803 ERR5262803_1.fastq ERR5262803_2.fastq Input file: ERR5262803_1.fastq Paired file: ERR5262803_2.fastq trimmed: ERR5262803-trimmed-pair1.fastq, ERR5262803-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:07:51 2024 >> started Fri Dec 6 12:08:27 2024 >> done (36.427s) 31012948 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 31012948 (100.00%) read pairs available; of these: 8846 ( 0.03%) trimmed read pairs available after processing 31004102 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 25 3 0.00% 26 0 0.00% 27 4 0.00% 28 0 0.00% 29 2 0.00% 30 4 0.00% 31 9 0.00% 32 3 0.00% 33 1 0.00% 34 4 0.00% 35 3 0.00% 36 2 0.00% 37 3 0.00% 38 1 0.00% 39 1 0.00% 40 0 0.00% 41 1 0.00% 42 0 0.00% 43 4 0.00% 44 4 0.00% 45 4 0.00% 46 0 0.00% 47 4 0.00% 48 3 0.00% 49 333 0.00% 50 378 0.00% 51 429 0.00% 52 420 0.00% 53 403 0.00% 54 490 0.00% 55 570 0.00% 56 633 0.00% 57 721 0.00% 58 787 0.00% 59 911 0.00% 60 1054 0.00% 61 1274 0.00% 62 1447 0.00% 63 1535 0.00% 64 1684 0.01% 65 1863 0.01% 66 2022 0.01% 67 2290 0.01% 68 2724 0.01% 69 3155 0.01% 70 3609 0.01% 71 4137 0.01% 72 4928 0.02% 73 5661 0.02% 74 6151 0.02% 75 6866 0.02% 76 7394 0.02% 77 8307 0.03% 78 8921 0.03% 79 10219 0.03% 80 11051 0.04% 81 12500 0.04% 82 14301 0.05% 83 16082 0.05% 84 17263 0.06% 85 19078 0.06% 86 20189 0.07% 87 21785 0.07% 88 22891 0.07% 89 24931 0.08% 90 27192 0.09% 91 28605 0.09% 92 31312 0.10% 93 33455 0.11% 94 36371 0.12% 95 38121 0.12% 96 40263 0.13% 97 41617 0.13% 98 43220 0.14% 99 44833 0.14% 100 47596 0.15% 101 49098 0.16% 102 52335 0.17% 103 55483 0.18% 104 57838 0.19% 105 60230 0.19% 106 62458 0.20% 107 63453 0.20% 108 65143 0.21% 109 67495 0.22% 110 68393 0.22% 111 70325 0.23% 112 73251 0.24% 113 76408 0.25% 114 79009 0.25% 115 81580 0.26% 116 82790 0.27% 117 84665 0.27% 118 85872 0.28% 119 86293 0.28% 120 87640 0.28% 121 90082 0.29% 122 92018 0.30% 123 93958 0.30% 124 97839 0.32% 125 98976 0.32% 126 101638 0.33% 127 102296 0.33% 128 102639 0.33% 129 103835 0.33% 130 104671 0.34% 131 103895 0.34% 132 107418 0.35% 133 108989 0.35% 134 110692 0.36% 135 113180 0.36% 136 114040 0.37% 137 114818 0.37% 138 115970 0.37% 139 119475 0.39% 140 118494 0.38% 141 123203 0.40% 142 124885 0.40% 143 126042 0.41% 144 128949 0.42% 145 128699 0.41% 146 132740 0.43% 147 261885 0.84% 148 122203 0.39% 149 121293 0.39% 150 25534335 82.33% 31012948 reads passed initial QC criterion=sequence-density sequence-density=2.54 sequence-density-rank=1 fanout-score=2.54 fanout-score-rank=27 prefix-density=2.93 prefix-fanout=2.2 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.04 sequence-density-rank=25 fanout-score=92.85 fanout-score-rank=1 prefix-density=0.81 prefix-fanout=4.5 sequence=CCGCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=9.74 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=37 prefix-density=9.73 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.05 sequence-density-rank=29 fanout-score=145.91 fanout-score-rank=1 prefix-density=1.57 prefix-fanout=4.6 sequence=AAGAAGAAGGTTGAAGCCAAGAACACCCTGGAGAACTATGCTTACAACATGAGGAACACCATCAGTGATGAGAAGATTGCTTCCAAGCTGCCAGCGGACGACAAGAAGAAGAT Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262803 ERR5262803_1.fastq ERR5262803_2.fastq Input file: ERR5262803_1.fastq Paired file: ERR5262803_2.fastq trimmed: ERR5262803-trimmed-pair1.fastq, ERR5262803-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:10:47 2024 >> started Fri Dec 6 12:11:13 2024 >> done (25.731s) 22152106 read pairs processed; of these: 113 ( 0.00%) short read pairs filtered out after trimming by size control 262 ( 0.00%) empty read pairs filtered out after trimming by size control 22151731 (100.00%) read pairs available; of these: 167 ( 0.00%) trimmed read pairs available after processing 22151564 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 25 1 0.00% 26 0 0.00% 27 2 0.00% 28 0 0.00% 29 1 0.00% 30 3 0.00% 31 4 0.00% 32 2 0.00% 33 1 0.00% 34 4 0.00% 35 1 0.00% 36 2 0.00% 37 3 0.00% 38 1 0.00% 39 1 0.00% 40 0 0.00% 41 1 0.00% 42 0 0.00% 43 1 0.00% 44 2 0.00% 45 4 0.00% 46 0 0.00% 47 4 0.00% 48 2 0.00% 49 154 0.00% 50 172 0.00% 51 198 0.00% 52 185 0.00% 53 196 0.00% 54 286 0.00% 55 473 0.00% 56 454 0.00% 57 615 0.00% 58 676 0.00% 59 767 0.00% 60 887 0.00% 61 1091 0.00% 62 1231 0.01% 63 1291 0.01% 64 1138 0.01% 65 928 0.00% 66 1168 0.01% 67 1888 0.01% 68 2281 0.01% 69 2651 0.01% 70 3073 0.01% 71 3379 0.02% 72 2744 0.01% 73 4735 0.02% 74 3930 0.02% 75 4361 0.02% 76 5467 0.02% 77 5485 0.02% 78 7402 0.03% 79 5602 0.03% 80 8539 0.04% 81 9001 0.04% 82 9270 0.04% 83 11753 0.05% 84 12967 0.06% 85 14036 0.06% 86 15309 0.07% 87 14635 0.07% 88 15675 0.07% 89 17699 0.08% 90 20930 0.09% 91 20492 0.09% 92 22375 0.10% 93 23037 0.10% 94 25424 0.11% 95 28110 0.13% 96 28694 0.13% 97 29052 0.13% 98 30714 0.14% 99 33214 0.15% 100 31819 0.14% 101 35734 0.16% 102 38523 0.17% 103 39023 0.18% 104 41362 0.19% 105 42850 0.19% 106 45518 0.21% 107 46577 0.21% 108 47273 0.21% 109 47918 0.22% 110 48767 0.22% 111 51358 0.23% 112 53035 0.24% 113 53197 0.24% 114 56324 0.25% 115 57750 0.26% 116 58898 0.27% 117 60378 0.27% 118 61287 0.28% 119 60826 0.27% 120 62786 0.28% 121 63183 0.29% 122 65149 0.29% 123 67170 0.30% 124 69443 0.31% 125 70188 0.32% 126 72041 0.33% 127 72791 0.33% 128 72805 0.33% 129 74288 0.34% 130 74513 0.34% 131 73875 0.33% 132 76817 0.35% 133 77888 0.35% 134 79147 0.36% 135 80588 0.36% 136 80765 0.36% 137 81695 0.37% 138 83111 0.38% 139 85429 0.39% 140 84740 0.38% 141 88187 0.40% 142 89310 0.40% 143 90366 0.41% 144 92259 0.42% 145 91666 0.41% 146 94693 0.43% 147 186135 0.84% 148 87452 0.39% 149 86642 0.39% 150 18242318 82.35% criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=3.82 fanout-score-rank=33 prefix-density=0.24 prefix-fanout=2.8 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.08 sequence-density-rank=13 fanout-score=149.30 fanout-score-rank=1 prefix-density=0.60 prefix-fanout=20.7 sequence=GCGGCGGCGGCGC criterion=sequence-density sequence-density=0.67 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=38 prefix-density=0.69 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=1036.55 fanout-score-rank=1 prefix-density=0.98 prefix-fanout=21.3 sequence=GCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTACGCGTGGGTGCTTGACAAGCTGAAGGCTGAGCGTGAGAGAGGTATCACCATCGATATTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGATGCCCCTGGACACCGTGACTTCATCAAGAACATGATTACCGGTACCTCCCAGGCTGACTGTGCCGTGCTTATCATTGACTCCACGACTGGAGGTTTTGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCATGCCCTCCTTGCTTTCACTCTTGGAGTGAAGCAGATGATCTGCTGCTGCAACAAGATGGA ERR5262803 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:12:11 Started mapping on | Dec 06 12:12:11 Finished on | Dec 06 12:14:33 Mapping speed, Million of reads per hour | 786.23 Number of input reads | 31012573 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 29826313 Uniquely mapped reads % | 96.17% Average mapped length | 289.85 Number of splices: Total | 29617286 Number of splices: Annotated (sjdb) | 27740760 Number of splices: GT/AG | 29201330 Number of splices: GC/AG | 348055 Number of splices: AT/AC | 20815 Number of splices: Non-canonical | 47086 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.50 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 364589 % of reads mapped to multiple loci | 1.18% Number of reads mapped to too many loci | 4864 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.62% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 821671 821671 821671 N_multimapping 364589 364589 364589 N_noFeature 967439 29069557 1205143 N_ambiguous 600480 4112 82586 UnstrandedReadsAssigned:28258394 PositiveStrandReadsAssigned:752644 NegativeStrandReadsAssigned:28538584 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262803 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262803-trimmed-pair1.fastq ERR5262803-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,012,573 reads, 28,929,873 reads pseudoaligned [quant] estimated average fragment length: 258.779 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,381 rounds 52973 ERR5262803.ke.tsv 35125 ERR5262803.se.tsv 88098 total ==> ERR5262803.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 678.733 0 0 PNS24247 1044 786.221 121.467 7.49472 PNS24249 1928 1670.22 404.022 11.7347 PNS24246 1044 786.221 121.467 7.49472 PNS24248 1044 786.221 121.467 7.49472 PNS24244 1471 1213.22 143.577 5.74099 PNS24243 293 109.079 1 0.444734 KQK14069 1603 1345.22 28712.3 1035.42 KQK14071 474 245.802 419.321 82.7566 ==> ERR5262803.se.tsv <== BRADI_1g14170v3 30631 BRADI_1g53295v3 183 BRADI_1g59795v3 728 BRADI_1g07683v3 0 BRADI_1g00485v3 54 BRADI_1g20270v3 1670 BRADI_1g74790v3 2046 BRADI_1g09890v3 0 BRADI_1g77505v3 353 BRADI_1g48960v3 0 ERR5262803 completed mapping pipeline successfully