Starting /dee2/code/volunteer_pipeline.sh ERR5262804 current disk space = 1551405350912 free memory = 1595191776 ERR5262804 SRAfilesize 43e5df460e677402cae40160ba9db73d ERR5262804.sra ERR5262804.sra file validated ERR5262804 is paired end ERR5262804 is conventional basespace ERR5262804 read1 length is 77-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262804_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 77-150 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.651 37.0 37.0 37.0 37.0 37.0 2 36.58225 37.0 37.0 37.0 37.0 37.0 3 36.6905 37.0 37.0 37.0 37.0 37.0 4 36.7175 37.0 37.0 37.0 37.0 37.0 5 36.73 37.0 37.0 37.0 37.0 37.0 6 36.713 37.0 37.0 37.0 37.0 37.0 7 36.7525 37.0 37.0 37.0 37.0 37.0 8 36.7285 37.0 37.0 37.0 37.0 37.0 9 36.719 37.0 37.0 37.0 37.0 37.0 10-14 36.769099999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.71379999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.703199999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.687799999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.6164 37.0 37.0 37.0 37.0 37.0 35-39 36.688700000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.7016 37.0 37.0 37.0 37.0 37.0 45-49 36.6519 37.0 37.0 37.0 37.0 37.0 50-54 36.653 37.0 37.0 37.0 37.0 37.0 55-59 36.6383 37.0 37.0 37.0 37.0 37.0 60-64 36.6115 37.0 37.0 37.0 37.0 37.0 65-69 36.548 37.0 37.0 37.0 37.0 37.0 70-74 36.562599999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.6192615403851 37.0 37.0 37.0 37.0 37.0 80-84 36.54014820770406 37.0 37.0 37.0 37.0 37.0 85-89 36.520493022419465 37.0 37.0 37.0 37.0 37.0 90-94 36.489918665220884 37.0 37.0 37.0 37.0 37.0 95-99 36.44119541141166 37.0 37.0 37.0 37.0 37.0 100-104 36.44889772455777 37.0 37.0 37.0 37.0 37.0 105-109 36.403942237525584 37.0 37.0 37.0 37.0 37.0 110-114 36.43594265079357 37.0 37.0 37.0 37.0 37.0 115-119 36.342490755740265 37.0 37.0 37.0 37.0 37.0 120-124 36.30796493093565 37.0 37.0 37.0 37.0 37.0 125-129 36.34138730773989 37.0 37.0 37.0 37.0 37.0 130-134 36.30530735658409 37.0 37.0 37.0 37.0 37.0 135-139 36.209433430904724 37.0 37.0 37.0 37.0 37.0 140-144 36.22987445887937 37.0 37.0 37.0 37.0 37.0 145-149 36.124401915486644 37.0 37.0 37.0 37.0 37.0 150 36.1496407372696 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 2.0 28 2.0 29 5.0 30 8.0 31 19.0 32 26.0 33 52.0 34 85.0 35 195.0 36 2911.0 37 694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.925 19.625 3.775 35.675000000000004 2 25.831457864466117 10.527631907976994 30.9827456864216 32.65816454113528 3 22.55 14.95 25.974999999999998 36.525 4 24.925 20.9 25.75 28.425 5 24.975 24.925 26.924999999999997 23.175 6 24.625 28.075 24.55 22.75 7 18.35 26.950000000000003 38.324999999999996 16.375 8 14.374999999999998 30.55 31.0 24.075 9 20.05 18.775 33.7 27.474999999999998 10-14 20.65 27.38 24.060000000000002 27.91 15-19 24.395 21.6 26.615 27.389999999999997 20-24 22.735 26.255 23.515 27.495000000000005 25-29 23.415 26.450000000000003 24.505 25.629999999999995 30-34 20.169999999999998 26.695 25.569999999999997 27.565 35-39 24.03 27.01 24.465 24.495 40-44 22.515 29.5 22.34 25.645 45-49 19.77 31.369999999999997 23.235 25.624999999999996 50-54 19.189999999999998 31.495 25.569999999999997 23.745 55-59 19.265 27.250000000000004 23.575 29.909999999999997 60-64 22.465 30.365 22.795 24.375 65-69 22.935 27.694999999999997 23.76 25.61 70-74 20.94 24.15 29.92 24.990000000000002 75-79 25.512551255125516 25.24252425242524 23.957395739573958 25.28752875287529 80-84 23.5029266096353 29.070989043974187 25.318925408974934 22.107158937415576 85-89 24.435770404844117 26.137216634139016 24.16053645598759 25.266476505029274 90-94 23.716503881793138 26.90708740295517 24.938642624593037 24.437766090658652 95-99 20.359221352598837 28.39654826409793 25.180614087898856 26.063616295404373 100-104 26.21754880257597 24.899376132018514 25.784866170255583 23.09820889514993 105-109 21.3630757681377 27.572523995924712 25.77081881066009 25.293581425277495 110-114 26.362206493652394 24.26131895292664 25.72744635434221 23.649028199078757 115-119 25.335522962795174 28.891783226683277 21.49612095815165 24.276572852369895 120-124 26.14828831928436 29.80102070072825 22.87401800561959 21.176672974367797 125-129 22.745508459794177 29.600558171986748 23.733938019652307 23.91999534856678 130-134 25.7533761868255 25.487999056436873 27.728961490829747 21.029663265907885 135-139 20.66782151603847 28.630308822650974 28.648228899109967 22.053640762200587 140-144 24.16949563530553 31.201503394762366 22.247817652764308 22.3811833171678 145-149 24.432766615146832 24.95208655332303 28.54404945904173 22.071097372488406 150 30.677913152139958 17.96313651983755 34.89534520462356 16.46360512339894 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 1.0 30 1.0 31 0.0 32 1.0 33 5.0 34 22.5 35 28.5 36 12.5 37 3.5 38 8.5 39 32.5 40 93.5 41 123.5 42 193.5 43 179.5 44 88.0 45 105.5 46 203.5 47 359.0 48 501.5 49 430.0 50 187.0 51 212.5 52 399.5 53 357.5 54 174.5 55 65.0 56 19.5 57 7.5 58 30.5 59 31.5 60 9.0 61 8.0 62 5.5 63 3.5 64 7.5 65 9.5 66 10.5 67 8.5 68 6.5 69 6.5 70 5.0 71 9.0 72 9.0 73 5.5 74 6.0 75 6.5 76 5.0 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 76-77 1.0 78-79 1.0 80-81 0.0 82-83 1.0 84-85 0.0 86-87 1.0 88-89 1.0 90-91 2.0 92-93 2.0 94-95 1.0 96-97 6.0 98-99 6.0 100-101 3.0 102-103 2.0 104-105 2.0 106-107 402.0 108-109 3.0 110-111 5.0 112-113 8.0 114-115 16.0 116-117 9.0 118-119 22.0 120-121 17.0 122-123 20.0 124-125 19.0 126-127 23.0 128-129 18.0 130-131 17.0 132-133 16.0 134-135 20.0 136-137 18.0 138-139 17.0 140-141 18.0 142-143 29.0 144-145 28.0 146-147 21.0 148-149 24.0 150-151 3201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.15 #Duplication Level Percentage of deduplicated Percentage of total 1 47.635933806146575 10.075000000000001 2 19.976359338061467 8.450000000000001 3 9.219858156028367 5.8500000000000005 4 4.7281323877068555 4.0 5 2.7186761229314422 2.875 6 1.6548463356973995 2.1 7 2.127659574468085 3.15 8 0.8274231678486997 1.4000000000000001 9 0.5910165484633569 1.125 >10 9.219858156028367 40.1 >50 1.0638297872340425 15.174999999999999 >100 0.2364066193853428 5.7 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGTTTCAA 116 2.9000000000000004 No Hit CCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGAAGAATCAT 112 2.8000000000000003 No Hit GCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACACTTGACAGAAC 78 1.95 No Hit GCACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGGCA 78 1.95 No Hit GCCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCATCA 76 1.9 No Hit CTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACATAGGCCT 72 1.7999999999999998 No Hit GTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACACTTGACAGA 68 1.7000000000000002 No Hit GTTTGATATGACGAAGAAGAAAGCCACCTATTACCTCTATCCTTTAGAAA 66 1.6500000000000001 No Hit GCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCT 64 1.6 No Hit CACCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGAAGAATC 54 1.35 No Hit GCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACACTTGAC 51 1.275 No Hit GTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGG 49 1.225 No Hit CCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGAAGAATCATG 48 1.2 No Hit CTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCATCATC 47 1.175 No Hit GTAAAGAACAGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACA 43 1.075 No Hit GCTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACATAGGCC 42 1.05 No Hit CTTTATTTTTCCCTGGCGCTATTCCTCCCTTGGTGGTGGGTGCTATACGG 42 1.05 No Hit CACTTCAACACCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAG 41 1.0250000000000001 No Hit CTCCTTTATTTTTCCCTGGCGCTATTCCTCCCTTGGTGGTGGGTGCTATA 40 1.0 No Hit GCCACCTATTACCTCTATCCTTTAGAAACATCCCCCTTGCGCACTGTTCA 36 0.8999999999999999 No Hit CCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCATCAT 34 0.8500000000000001 No Hit CTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGA 32 0.8 No Hit CCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTT 31 0.775 No Hit CTGGTTACGGCGCAGGCACCGAACAACAAATCCACACACTTGACAGAACA 30 0.75 No Hit CCAGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGTTTCAATC 30 0.75 No Hit AGGTAAAGAACAGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAA 29 0.7250000000000001 No Hit ACTTCAACACCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGG 29 0.7250000000000001 No Hit GGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGTTT 29 0.7250000000000001 No Hit ATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACACTTG 27 0.675 No Hit CTTCAACACCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGA 26 0.65 No Hit CACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGGCAA 26 0.65 No Hit GGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCG 25 0.625 No Hit CACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGATT 25 0.625 No Hit GGTAAAGAACAGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAAC 25 0.625 No Hit GGGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCT 25 0.625 No Hit CAGGTAAAGAACAGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACA 25 0.625 No Hit GTCGATATCCCTAGTGCCCGTATGAATTTGCGGTAGTACCATTTGTCAAA 25 0.625 No Hit GCTGAGGGGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGAT 22 0.5499999999999999 No Hit CCTTTATTTTTCCCTGGCGCTATTCCTCCCTTGGTGGTGGGTGCTATACG 21 0.525 No Hit ATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGA 21 0.525 No Hit CGCCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCATC 20 0.5 No Hit CCTGGCGCTATTCCTCCCTTGGTGGTGGGTGCTATACGGCTCTATACGCA 20 0.5 No Hit GTACTCCTGATGCTACCGGTTGTCAGAGCATCATCCATCATAAATGACAG 19 0.475 No Hit GCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACAT 19 0.475 No Hit ACCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGAAGAATCA 19 0.475 No Hit CTTTTCTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCATCATCCA 18 0.44999999999999996 No Hit CATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACACTT 18 0.44999999999999996 No Hit CTCTGCGCTATCCCTCACAATCAATTTGCCTTCTGGCCTCAATATCCGGT 18 0.44999999999999996 No Hit CCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGGCAAAT 18 0.44999999999999996 No Hit CTCAACTGTTGTCAAAGTTGTATGACCTTCAGGCTACCATGGACCGGTCA 17 0.42500000000000004 No Hit GGGGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGC 17 0.42500000000000004 No Hit CTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTAC 17 0.42500000000000004 No Hit GATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACA 16 0.4 No Hit AGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACAC 16 0.4 No Hit CTTGGCGTAGGTCATGGTTACCTCCCAGTGTAGGGACTTTGCCATGCCTT 15 0.375 No Hit GTGGCTGAGGGGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACA 15 0.375 No Hit CAGGCACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAG 15 0.375 No Hit ATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACAC 15 0.375 No Hit GGCTGAGGGGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGA 14 0.35000000000000003 No Hit CCTCTATCCTTTAGAAACATCCCCCTTGCGCACTGTTCATAATAAGAACA 14 0.35000000000000003 No Hit CTGTACTCCTGATGCTACCGGTTGTCAGAGCATCATCCATCATAAATGAC 14 0.35000000000000003 No Hit CTCCAACTTAACATTGCCTGACAGTATTTCTTCGACAACTTTCTCGATAC 14 0.35000000000000003 No Hit GCGCAGGCACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAA 14 0.35000000000000003 No Hit CTTTGCTAAACGCCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTTG 14 0.35000000000000003 No Hit GCCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGAAGAATCA 14 0.35000000000000003 No Hit CCTGATGCTACCGGTTGTCAGAGCATCATCCATCATAAATGACAGGAGTC 13 0.325 No Hit GGAGGAGGAGCTCGCCATGGCGAACCTAAATTTAATCTCCTTTATTTTTC 13 0.325 No Hit GGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACATAGGCCTCGT 13 0.325 No Hit GGGCCATCAAATTTCTCTCTGTCTCGCTGCCCTTGAATTCCAGAACTAGC 12 0.3 No Hit GGTTTGATATGACGAAGAAGAAAGCCACCTATTACCTCTATCCTTTAGAA 12 0.3 No Hit GGATGGATTACAGCGTGAAAAGTATAAAGAGTCGTGATCTTGCATTACTC 12 0.3 No Hit CTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCATCATCCATCATA 12 0.3 No Hit GCGGTAGTACCATTTGTCAAATGCTCCAACTTAACATTGCCTGACAGTAT 12 0.3 No Hit CTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACATA 12 0.3 No Hit ACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGGCAAA 11 0.27499999999999997 No Hit CATCTGTACTCCTGATGCTACCGGTTGTCAGAGCATCATCCATCATAAAT 11 0.27499999999999997 No Hit GTTACGGCGCAGGCACCGAACAACAAATCCACACACTTGACAGAACAGTC 11 0.27499999999999997 No Hit GCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTA 11 0.27499999999999997 No Hit CTCAGCTGCAGTGGCTGAGGGGACAAGGTGGGAGTCCAGAAGCTTGAGGA 11 0.27499999999999997 No Hit GTGGTTTGATATGACGAAGAAGAAAGCCACCTATTACCTCTATCCTTTAG 11 0.27499999999999997 No Hit GCAGGTAAAGAACAGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAAC 11 0.27499999999999997 No Hit TGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGT 11 0.27499999999999997 No Hit GGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCTA 10 0.25 No Hit CTCCTGATGCTACCGGTTGTCAGAGCATCATCCATCATAAATGACAGGAG 10 0.25 No Hit CAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACACACTTGACA 10 0.25 No Hit GCTCAACGAAGAAGAAGAAGAGGAGGAGGAGCTCGCCATGGCGAACCTAA 10 0.25 No Hit CAGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCC 10 0.25 No Hit GAACAGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCC 10 0.25 No Hit GCGCCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCAT 10 0.25 No Hit GGCGAACCTAAATTTAATCTCCTTTATTTTTCCCTGGCGCTATTCCTCCC 9 0.22499999999999998 No Hit CGCTTTTTCTCTGTCACAAAATTGCTTGCTCTCAAAATGAAAATTTACGA 9 0.22499999999999998 No Hit TCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCG 9 0.22499999999999998 No Hit GACGAAGAAGAAAGCCACCTATTACCTCTATCCTTTAGAAACATCCCCCT 9 0.22499999999999998 No Hit GTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACATAGGCCTCGTT 9 0.22499999999999998 No Hit GGCGCAGGCACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCA 8 0.2 No Hit GTCTCTGCGCTATCCCTCACAATCAATTTGCCTTCTGGCCTCAATATCCG 8 0.2 No Hit TGGCGCTATTCCTCCCTTGGTGGTGGGTGCTATACGGCTCTATACGCAGA 8 0.2 No Hit GTCACAAAATTGCTTGCTCTCAAAATGAAAATTTACGAAGGTAAAAAAAC 8 0.2 No Hit GCCCTCTTGTTGCTCAACGAAGAAGAAGAAGAGGAGGAGGAGCTCGCCAT 8 0.2 No Hit GCTTGCTCTCAAAATGAAAATTTACGAAGGTAAAAAAACATGATTTACCA 8 0.2 No Hit AGCGTGCAAGCATTTGTATCAAACCATCGATGCTTATTTCTCCAGCAAAC 8 0.2 No Hit CGGCGCAGGCACCGAACAACAAATCCACACACTTGACAGAACAGTCACTC 7 0.17500000000000002 No Hit AGCAAGTGATACGGATGGATTACAGCGTGAAAAGTATAAAGAGTCGTGAT 7 0.17500000000000002 No Hit CGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGGCAAATG 7 0.17500000000000002 No Hit GCTATCCCTCACAATCAATTTGCCTTCTGGCCTCAATATCCGGTCAACCT 7 0.17500000000000002 No Hit CCTTGGCGTAGGTCATGGTTACCTCCCAGTGTAGGGACTTTGCCATGCCT 7 0.17500000000000002 No Hit AACAGATCATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCA 7 0.17500000000000002 No Hit CTTGCTCTCAAAATGAAAATTTACGAAGGTAAAAAAACATGATTTACCAC 7 0.17500000000000002 No Hit GTGCATATCAATGAAGAGTGGTTTGATATGACGAAGAAGAAAGCCACCTA 7 0.17500000000000002 No Hit CCCTTGCGCACTGTTCATAATAAGAACAGCATTGGGAAAGTAATGTTTCT 7 0.17500000000000002 No Hit GCCTTTGCTAAACGCCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGT 7 0.17500000000000002 No Hit CCCTAGTGCCCGTATGAATTTGCGGTAGTACCATTTGTCAAATGCTCCAA 7 0.17500000000000002 No Hit CATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTG 7 0.17500000000000002 No Hit GTCGGCTTCCCTCGCCCTCTTGTTGCTCAACGAAGAAGAAGAAGAGGAGG 7 0.17500000000000002 No Hit GGTTACGGCGCAGGCACCGAACAACAAATCCACACACTTGACAGAACAGT 7 0.17500000000000002 No Hit CTTCGAGTTCGATTATTGTCTCTGCGCTATCCCTCACAATCAATTTGCCT 7 0.17500000000000002 No Hit AGTCGATATCCCTAGTGCCCGTATGAATTTGCGGTAGTACCATTTGTCAA 7 0.17500000000000002 No Hit GCAGTGGCTGAGGGGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATC 7 0.17500000000000002 No Hit GGATGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTAC 7 0.17500000000000002 No Hit AGGAGAGCTATGAGGGAGGCTGCTGGCCTCCGGCGTGCGAGCGCCATTGT 6 0.15 No Hit CCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCG 6 0.15 No Hit AGGTCTCTGACTTTTCAGGGTAGTTCAGATCATCAACAGTTGATATTGAA 6 0.15 No Hit AGTATTGTTTGGCGGGAAAGAGAAAGAAGACAGTAGCTGGCCGACGCTTG 6 0.15 No Hit AACCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGAAGAATC 6 0.15 No Hit ACTCCTGATGCTACCGGTTGTCAGAGCATCATCCATCATAAATGACAGGA 6 0.15 No Hit AGAAGCTTGAGGATGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCT 6 0.15 No Hit CGAAGAAGAAAGCCACCTATTACCTCTATCCTTTAGAAACATCCCCCTTG 6 0.15 No Hit GCATCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGGCA 6 0.15 No Hit CGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCC 6 0.15 No Hit CCTCGCCCTCTTGTTGCTCAACGAAGAAGAAGAAGAGGAGGAGGAGCTCG 6 0.15 No Hit CTGAGGGGACAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATC 6 0.15 No Hit ATCCCATCTTCATAGCCTCCTTGAATGAGGTAGCTCCAGTAGGAAGAATC 6 0.15 No Hit GGCACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGGC 6 0.15 No Hit GGGGCAAGGTGGGAGTCCAGAAGCTTGAGGATGCCATCACAGATCTTGCT 5 0.125 No Hit GGATTACAGCGTGAAAAGTATAAAGAGTCGTGATCTTGCATTACTCGATA 5 0.125 No Hit CCGCCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTTGTCAGAGCAT 5 0.125 No Hit GAAGAAGAAAGCCACCTATTACCTCTATCCTTTAGAAACATCCCCCTTGC 5 0.125 No Hit ACGGCGCAGGCACCGAACAACAAATCCACACACTTGACAGAACAGTCACT 5 0.125 No Hit GTGACGTTCAGAACCGATTGGTTCCAGTGCAAACGAGCATTTCTTTCCAG 5 0.125 No Hit CCTCAATATCCGGTCAACCTCGGCAAAGACTCCCAACAATTTGCATCTCT 5 0.125 No Hit ACGAAGAAGAAAGCCACCTATTACCTCTATCCTTTAGAAACATCCCCCTT 5 0.125 No Hit AGGAGGAGGAGCTCGCCATGGCGAACCTAAATTTAATCTCCTTTATTTTT 5 0.125 No Hit GGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCC 5 0.125 No Hit ACCACTCTTTTATACAGCAAGTGATACGGATGGATTACAGCGTGAAAAGT 5 0.125 No Hit GCTCCTGATGCTACCGGTTGTCAGAGCATCATCCATCATAAATGACAGGA 5 0.125 No Hit CCTTTGCTAAACGCCTCTTTTCTCCATCTGTACTCCTGATGCTACCGGTT 5 0.125 No Hit ATCTCCTTTATTTTTCCCTGGCGCTATTCCTCCCTTGGTGGTGGGTGCTA 5 0.125 No Hit AGGCACCGAACAACAAATCCACACACTTGACAGAACAGTCACTCAAAAGG 5 0.125 No Hit GCCCGTATGAATTTGCGGTAGTACCATTTGTCAAATGCTCCAACTTAACA 5 0.125 No Hit GATGGATTACAGCGTGAAAAGTATAAAGAGTCGTGATCTTGCATTACTCG 5 0.125 No Hit CGATTATGCAGTGCTGGTTACGGCGCAGGCACCGAACAACAAATCCACAC 5 0.125 No Hit GATGCCATCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACT 5 0.125 No Hit CCACCACTCTTTTATACAGCAAGTGATACGGATGGATTACAGCGTGAAAA 5 0.125 No Hit TGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACA 5 0.125 No Hit GCCTTCACAGATCTTGCTAAGCTCGGTTTCAATCCTGGTACGGTACTCCT 5 0.125 No Hit GTTCGGTTTCAATCCTGGTACGGTACTCCTTGATTGAAGCGACATAGGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.025 0.0 0.0 0.0 0.0 122-123 0.05 0.0 0.0 0.0 0.0 124-125 0.05 0.0 0.0 0.0 0.0 126-127 0.05 0.0 0.0 0.0 0.0 128-129 0.05 0.0 0.0 0.0 0.0 130-131 0.05 0.0 0.0 0.0 0.0 132-133 0.05 0.0 0.0 0.0 0.0 134-135 0.05 0.0 0.0 0.0 0.0 136-137 0.05 0.0 0.0 0.0 0.0 138 0.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGATTG 20 0.0027615381 33.867695 140-144 TACCAGC 30 1.4338038E-5 33.867695 135-139 GCAAGAT 35 4.1130297E-5 29.029451 140-144 AGCAAGA 30 5.787329E-4 28.223078 140-144 ACCAGCA 30 5.787329E-4 28.223078 135-139 CTTGTTA 40 1.18197655E-4 24.865211 130-134 TTACCAG 35 0.0014236453 24.19121 135-139 GAACCAA 40 1.537606E-4 23.92826 120-124 AGGCAGA 40 1.537606E-4 23.92826 115-119 GGAACAG 40 1.730083E-4 23.519232 110-114 ATGCAGG 40 1.9800931E-4 23.059357 105-109 GTTGAAT 40 2.3899406E-4 22.432745 100-104 AAGTGCT 45 2.9687583E-4 21.71006 125-129 TTGTTAC 40 0.0035048593 20.72101 130-134 AGCCTCC 40 4.2176544E-4 20.638126 10-14 AGCTTGT 40 4.2176544E-4 20.638126 65-69 CGTTAAT 40 4.2176544E-4 20.638126 90-94 ATGACGT 40 4.2176544E-4 20.638126 95-99 CTCCAGT 40 4.2176544E-4 20.638126 30-34 GTTTCCG 40 4.2176544E-4 20.638126 70-74 >>END_MODULE ERR5262804 read2 length is 77-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262804_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 77-150 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.24 37.0 37.0 37.0 37.0 37.0 2 36.103 37.0 37.0 37.0 37.0 37.0 3 36.0515 37.0 37.0 37.0 37.0 37.0 4 36.1975 37.0 37.0 37.0 37.0 37.0 5 36.2615 37.0 37.0 37.0 37.0 37.0 6 36.2115 37.0 37.0 37.0 37.0 37.0 7 36.246 37.0 37.0 37.0 37.0 37.0 8 36.3205 37.0 37.0 37.0 37.0 37.0 9 36.322 37.0 37.0 37.0 37.0 37.0 10-14 36.3302 37.0 37.0 37.0 37.0 37.0 15-19 36.3319 37.0 37.0 37.0 37.0 37.0 20-24 36.293400000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.2375 37.0 37.0 37.0 37.0 37.0 30-34 36.184 37.0 37.0 37.0 37.0 37.0 35-39 36.1803 37.0 37.0 37.0 37.0 37.0 40-44 36.22709999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.1582 37.0 37.0 37.0 37.0 37.0 50-54 36.1309 37.0 37.0 37.0 37.0 37.0 55-59 36.10635 37.0 37.0 37.0 37.0 37.0 60-64 36.0755 37.0 37.0 37.0 37.0 37.0 65-69 36.0811 37.0 37.0 37.0 37.0 37.0 70-74 35.9839 37.0 37.0 37.0 37.0 37.0 75-79 36.083805676419104 37.0 37.0 37.0 37.0 37.0 80-84 35.96473363026272 37.0 37.0 37.0 37.0 37.0 85-89 35.95661303034333 37.0 37.0 37.0 37.0 37.0 90-94 35.871867201066145 37.0 37.0 37.0 37.0 37.0 95-99 35.86687725980922 37.0 37.0 37.0 37.0 37.0 100-104 35.79467398196731 37.0 37.0 37.0 37.0 37.0 105-109 35.72769098220474 37.0 37.0 37.0 37.0 37.0 110-114 35.66424312727554 37.0 37.0 37.0 37.0 37.0 115-119 35.6469686307895 37.0 37.0 37.0 37.0 37.0 120-124 35.634340027057725 37.0 37.0 37.0 37.0 37.0 125-129 35.64098420155922 37.0 37.0 37.0 37.0 37.0 130-134 35.395643182438604 37.0 37.0 37.0 34.6 37.0 135-139 35.51482116306239 37.0 37.0 37.0 37.0 37.0 140-144 35.50574284890173 37.0 37.0 37.0 34.6 37.0 145-149 35.489762473469916 37.0 37.0 37.0 37.0 37.0 150 35.57840375586854 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 3.0 14 2.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 3.0 22 4.0 23 3.0 24 8.0 25 4.0 26 6.0 27 8.0 28 13.0 29 12.0 30 26.0 31 37.0 32 58.0 33 76.0 34 180.0 35 569.0 36 2705.0 37 279.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.55 19.375 7.000000000000001 30.075000000000003 2 28.549999999999997 23.625 24.625 23.200000000000003 3 23.0 23.849999999999998 30.275000000000002 22.875 4 27.700000000000003 28.325 22.675 21.3 5 25.75 34.300000000000004 17.675 22.275 6 25.224999999999998 29.875 22.075 22.825 7 21.825 20.075000000000003 33.475 24.625 8 24.85 20.4 25.924999999999997 28.825 9 22.7 19.825 27.525 29.95 10-14 25.665 23.595 24.349999999999998 26.39 15-19 26.290000000000003 25.485000000000003 23.335 24.89 20-24 26.72 23.849999999999998 24.635 24.795 25-29 25.82 24.395 23.785 26.0 30-34 27.18 23.82 24.895 24.104999999999997 35-39 26.93 25.474999999999998 24.39 23.205000000000002 40-44 25.480000000000004 24.65 24.695 25.174999999999997 45-49 26.43 23.815 25.605 24.15 50-54 26.265 23.695 25.505 24.535 55-59 26.66133306665333 24.98624931246562 25.056252812640633 23.296164808240412 60-64 25.345000000000002 24.915000000000003 25.145 24.595 65-69 27.08 23.985 25.56 23.375 70-74 26.240000000000002 25.169999999999998 25.509999999999998 23.080000000000002 75-79 25.802580258025802 24.557455745574558 25.6025602560256 24.03740374037404 80-84 26.159387663214765 25.378958427134922 24.42343288808845 24.03822102156186 85-89 26.427463343842266 23.104638943101634 25.957063503978382 24.510834209077718 90-94 24.992485722873457 26.104598737601442 24.81214307183649 24.09077246768861 95-99 25.19195061976213 25.06147438149245 25.623525869423396 24.123049129322023 100-104 26.401610468042275 27.131353799698037 23.024660291897334 23.442375440362355 105-109 25.929103877299298 24.94234997586743 25.355285032444897 23.773261114388372 110-114 25.261206605999327 27.075609482080665 25.042130097741826 22.621053814178183 115-119 26.686300050971283 26.244548904117348 24.211360933340885 22.85779011157048 120-124 26.035333256854422 26.322129172880576 24.016290008030285 23.626247562234713 125-129 26.68643870667597 26.16306117701791 23.732263317050478 23.41823679925564 130-134 26.713864306784664 26.589970501474923 24.761061946902654 21.93510324483776 135-139 27.826606875934228 24.837070254110614 26.31390134529148 21.022421524663677 140-144 27.830360393156173 25.476277150831212 24.7421429438175 21.951219512195124 145-149 25.579090796482102 26.155084850737026 25.826830174656262 22.438994178124613 150 23.380281690140844 26.040688575899846 27.636932707355243 22.94209702660407 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.5 28 1.5 29 1.5 30 2.5 31 4.0 32 5.0 33 6.5 34 7.0 35 8.0 36 12.0 37 13.0 38 26.5 39 60.5 40 80.5 41 83.5 42 139.5 43 166.5 44 158.5 45 199.0 46 178.0 47 130.0 48 147.0 49 190.5 50 198.0 51 213.5 52 247.5 53 244.0 54 229.5 55 265.0 56 264.0 57 158.5 58 85.5 59 58.0 60 52.5 61 57.5 62 41.0 63 37.5 64 33.5 65 29.5 66 33.0 67 30.5 68 19.5 69 13.0 70 15.0 71 11.5 72 8.0 73 7.0 74 7.0 75 7.0 76 5.0 77 2.5 78 3.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.005 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 76-77 1.0 78-79 1.0 80-81 0.0 82-83 1.0 84-85 0.0 86-87 1.0 88-89 1.0 90-91 3.0 92-93 2.0 94-95 1.0 96-97 6.0 98-99 6.0 100-101 3.0 102-103 2.0 104-105 2.0 106-107 401.0 108-109 3.0 110-111 5.0 112-113 8.0 114-115 16.0 116-117 10.0 118-119 22.0 120-121 17.0 122-123 20.0 124-125 18.0 126-127 24.0 128-129 18.0 130-131 17.0 132-133 17.0 134-135 21.0 136-137 18.0 138-139 16.0 140-141 19.0 142-143 29.0 144-145 31.0 146-147 21.0 148-149 24.0 150-151 3195.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.775 #Duplication Level Percentage of deduplicated Percentage of total 1 51.099937146448774 20.325 2 19.610307982401007 15.6 3 10.370835952231301 12.375 4 5.782526712759271 9.2 5 3.331238214959145 6.625 6 2.95411690760528 7.049999999999999 7 1.8856065367693273 5.25 8 1.3199245757385292 4.2 9 0.5656819610307983 2.025 >10 3.0798240100565684 17.349999999999998 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATGAGCTTAGCAAGAGCACTGGGAATGCTGGTGCACGTGTTGCTTGTGG 34 0.8500000000000001 No Hit GGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAGCAAG 30 0.75 No Hit CTGCAGCGCACACACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAG 23 0.575 No Hit AGCAAATCCTCTCTCCTCCTTCCTCCTCCGCTCGATTTGACATAGGCTTC 21 0.525 No Hit AGGAAAGGTACCGAGCAAGTTCGTGTAACAAAGGAGAGGAAAAATGTCTA 21 0.525 No Hit GCCTACTCTTTCTTTCCCACAATCCCACGAGCTGAGCTTCGCGTTGCGTT 20 0.5 No Hit CCCCCTTGCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCACGAGCT 18 0.44999999999999996 No Hit GGTTGGTGCAAGCAGAAGCTTGGTGCTAATGCAATCCTGGCTGTGTCACT 18 0.44999999999999996 No Hit GGAAATGGTTGAATTCATGGAGAAGGTGGCCAAGACTGCCGATGTCGGTG 17 0.42500000000000004 No Hit CATCACCCCCTTGCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCAC 16 0.4 No Hit GTTTGCCCCTCACAAAAGGATATGCCTATCAATGAGTGACTTTCATCCAG 16 0.4 No Hit GGGGTAATCGCTTGCCAACACCGTACAGATCTGGGCACATTCGACATTCA 16 0.4 No Hit CGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGA 15 0.375 No Hit CTTGCCTACTCTTTCTTTCCCACAATCCCACGAGCTGAGCTTCGCGTTGC 15 0.375 No Hit GTCACTAGCTGTTTGCAAAGCCGGAGCTTCCGTGAAGAAGATTCCGCTGT 15 0.375 No Hit CTTTCTTTCCCACAATCCCACGAGCTGAGCTTCGCGTTGCGTTTCTCTCT 15 0.375 No Hit GAACTTTATTGCCGCCATTTTAAATAAGCACCTGATCCATGATCGCTGAT 15 0.375 No Hit CACGAACCTGCAGCGCACACACGAGATCCGCAGCCGAGAGGGGAGCCCGT 14 0.35000000000000003 No Hit GCCAAGACTGCCGATGTCGGTGAGCTCACTGTTGAGGAGCGTAACCTACT 14 0.35000000000000003 No Hit AGAATGTCTACATGGCCAAGCTCGCCGAGCAGGCTGAGCGTTACGAGGAA 14 0.35000000000000003 No Hit CCTTGCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCACGAGCTGAG 14 0.35000000000000003 No Hit CCCCTTGCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCACGAGCTG 13 0.325 No Hit CTTTATTGCCGCCATTTTAAATAAGCACCTGATCCATGATCGCTGATCAG 13 0.325 No Hit GGCACATTCGACATTCAGGATATGAACTTTATTGCCGCCATTTTAAATAA 13 0.325 No Hit CGAACCTGCAGCGCACACACGAGATCCGCAGCCGAGAGGGGAGCCCGTGA 13 0.325 No Hit GAAAGGTACCGAGCAAGTTCGTGTAACAAAGGAGAGGAAAAATGTCTACT 13 0.325 No Hit GGAGAATGTCTACATGGCCAAGCTCGCCGAGCAGGCTGAGCGTTACGAGG 12 0.3 No Hit GGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAGCAAGT 12 0.3 No Hit CATTCATATCCGGAGAGTTTGCTGGCTGCTGCATCAGTTGAATGTCCAGG 12 0.3 No Hit GTACCGAGCAAGTTCGTGTAACAAAGGAGAGGAAAAATGTCTACTGCTGA 11 0.27499999999999997 No Hit GGCTGAGCGTTACGAGGAAATGGTTGAATTCATGGAGAAGGTGGCCAAGA 11 0.27499999999999997 No Hit GCTGGAACAACTTTACCACACATAAACTGCCTCATGACTTCCCGGTTCAC 11 0.27499999999999997 No Hit CACATCATTTCCTATGTGGCTGTTGGAACATTTGCATCACCATCATTTGC 11 0.27499999999999997 No Hit GGTGAGCTCACTGTTGAGGAGCGTAACCTACTTTCTGTGGCTTATAAGAA 11 0.27499999999999997 No Hit GGTGGCCAAGACTGCCGATGTCGGTGAGCTCACTGTTGAGGAGCGTAACC 11 0.27499999999999997 No Hit CCTGCAGCGCACACACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAA 11 0.27499999999999997 No Hit AGCAGGCTGAGCGTTACGAGGAAATGGTTGAATTCATGGAGAAGGTGGCC 11 0.27499999999999997 No Hit GAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAGCAAGTTC 11 0.27499999999999997 No Hit GAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAG 11 0.27499999999999997 No Hit GAGCAAGTTCGTGTAACAAAGGAGAGGAAAAATGTCTACTGCTGAGGCAA 11 0.27499999999999997 No Hit CGGGCTCCAGGGCTAGATGGGGTAATCGCTTGCCAACACCGTACAGATCT 11 0.27499999999999997 No Hit GTTCTGTATAGCTGGAACAACTTTACCACACATAAACTGCCTCATGACTT 10 0.25 No Hit CACTAGCTGTTTGCAAAGCCGGAGCTTCCGTGAAGAAGATTCCGCTGTAC 10 0.25 No Hit GTCTACATGGCCAAGCTCGCCGAGCAGGCTGAGCGTTACGAGGAAATGGT 10 0.25 No Hit GCTCAAATTCCCATCCGATTATCCTTTTAAACCTCCAAGTATCAGCATGA 10 0.25 No Hit ACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGG 10 0.25 No Hit GCGTAACCTACTTTCTGTGGCTTATAAGAACGTGATTGGTGCCCGGAGGG 10 0.25 No Hit GAGGGACGGTGGGTCTGACTACTTGGGCAAGGGTGTTTCCAAGGCTGTTA 10 0.25 No Hit GGAAAAATGTCTACTGCTGAGGCAACCCGTGAGGAGAATGTCTACATGGC 10 0.25 No Hit GTTGAATTCATGGAGAAGGTGGCCAAGACTGCCGATGTCGGTGAGCTCAC 9 0.22499999999999998 No Hit GCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCACGAGCTGAGCTTC 9 0.22499999999999998 No Hit CACAATCCCACGAGCTGAGCTTCGCGTTGCGTTTCTCTCTCGCGTCATCT 9 0.22499999999999998 No Hit CAGCACATTGCCAATCTTGCTGGTAACAAGCACTTGGTTCTGCCTGTTCC 9 0.22499999999999998 No Hit GGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAGCAAGTTCGTGTAACAAA 9 0.22499999999999998 No Hit GTCGGTGAGCTCACTGTTGAGGAGCGTAACCTACTTTCTGTGGCTTATAA 9 0.22499999999999998 No Hit CCCAGATCCCCCTGACTGGACCGCATTCGATCATTGGCCGTGCTGTTGTT 9 0.22499999999999998 No Hit CCCCACGAACCTGCAGCGCACACACGAGATCCGCAGCCGAGAGGGGAGCC 9 0.22499999999999998 No Hit GTTACGGCCTAGGGTTTTGCCATGGCCGACAGGGGCTGCCTGAAGCGCCT 9 0.22499999999999998 No Hit CTCTTTCTTTCCCACAATCCCACGAGCTGAGCTTCGCGTTGCGTTTCTCT 8 0.2 No Hit CCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAG 8 0.2 No Hit ATTGAGGGTTGCCGATCGAGGGGCGCGCGTGCGGGGGGATTTGAGCTAGG 8 0.2 No Hit GGCGGATTCTACTATGGGAAGCTCAAATTCCCATCCGATTATCCTTTTAA 8 0.2 No Hit ACGTGATTGGTGCCCGGAGGGCATCATGGAGGATCATCTCATCCATTGAG 8 0.2 No Hit GCTCACTGTTGAGGAGCGTAACCTACTTTCTGTGGCTTATAAGAACGTGA 8 0.2 No Hit CCTACTCTTTCTTTCCCACAATCCCACGAGCTGAGCTTCGCGTTGCGTTT 8 0.2 No Hit GTTGAGGAGCGTAACCTACTTTCTGTGGCTTATAAGAACGTGATTGGTGC 8 0.2 No Hit GAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAG 8 0.2 No Hit GCTGAGCTTCGCGTTGCGTTTCTCTCTCGCGTCATCTCTCGGTGCTCTCT 8 0.2 No Hit CCCTTGCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCACGAGCTGA 8 0.2 No Hit GGGAATGCTGGTGCACGTGTTGCTTGTGGGATCATCGGGCTCCAGGGCTA 8 0.2 No Hit AGAAGATTCCGCTGTACCAGCACATTGCCAATCTTGCTGGTAACAAGCAC 8 0.2 No Hit GCATGACAACCCCCAGCGGCAGGTTTGCCCCTCACAAAAGGATATGCCTA 8 0.2 No Hit GCTGGAGTTTCCTTCACAAATGGCCACACCCTATTTTGCCATCAAAGATT 8 0.2 No Hit CTTGCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCACGAGCTGAGC 8 0.2 No Hit AGCTGAGCTTCGCGTTGCGTTTCTCTCTCGCGTCATCTCTCGGTGCTCTC 8 0.2 No Hit CCTGAGGGAGGCTCTGATGACATTGAAGCACAGGCAAATTTATATTTTCA 8 0.2 No Hit GAAACTCGCCATGCTGGTGATCTTGGAAATGTGACGGCTGGAGTGGATGG 8 0.2 No Hit CTTTTAAACCTCCAAGTATCAGCATGACAACCCCCAGCGGCAGGTTTGCC 8 0.2 No Hit CTTTCATCCAGAATCTTGGAATCCAATGTGGTCTGTAGCAAGCATTCTCA 8 0.2 No Hit CTTTGAAGGCGGATTCTACTATGGGAAGCTCAAATTCCCATCCGATTATC 7 0.17500000000000002 No Hit AATGTCTACATGGCCAAGCTCGCCGAGCAGGCTGAGCGTTACGAGGAAAT 7 0.17500000000000002 No Hit AAGGAGAGGAAAAATGTCTACTGCTGAGGCAACCCGTGAGGAGAATGTCT 7 0.17500000000000002 No Hit GGAGAGGAAAAATGTCTACTGCTGAGGCAACCCGTGAGGAGAATGTCTAC 7 0.17500000000000002 No Hit CTCAAATTCCCATCCGATTATCCTTTTAAACCTCCAAGTATCAGCATGAC 7 0.17500000000000002 No Hit CACGAGCTGAGCTTCGCGTTGCGTTTCTCTCTCGCGTCATCTCTCGGTGC 7 0.17500000000000002 No Hit GTTTCCTTCACAAATGGCCACACCCTATTTTGCCATCAAAGATTATAGCA 7 0.17500000000000002 No Hit GGTTTGGGAAGGTTGAGGGCGGGGATCTGTCATCGCTGTCTCCGGTGCCG 7 0.17500000000000002 No Hit ACACCGTACAGATCTGGGCACATTCGACATTCAGGATATGAACTTTATTG 7 0.17500000000000002 No Hit GCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGT 7 0.17500000000000002 No Hit CATTCACATCATTTCCTATGTGGCTGTTGGAACATTTGCATCACCATCAT 7 0.17500000000000002 No Hit GATCCATGATCGCTGATCAGTGTGCACCATTCACATCATTTCCTATGTGG 7 0.17500000000000002 No Hit CGGTGAGCTCACTGTTGAGGAGCGTAACCTACTTTCTGTGGCTTATAAGA 7 0.17500000000000002 No Hit AGGGTGTCAAGGGCACCATCTTTTTCACCCAAGAGGGAGATGGTCCGACC 7 0.17500000000000002 No Hit CCTGACTGGACCGCATTCGATCATTGGCCGTGCTGTTGTTGTCCATGGTG 7 0.17500000000000002 No Hit GTACTCTGCAATCTGTTAGGGATTGAATGGACCTGAAAAATCCTAAGTCG 7 0.17500000000000002 No Hit GTACAGATCTGGGCACATTCGACATTCAGGATATGAACTTTATTGCCGCC 7 0.17500000000000002 No Hit GAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAGCA 7 0.17500000000000002 No Hit GCCTGTTCCTGCATTCAACGTCATTAACGGTGGATCCCATGCCGGAAACA 7 0.17500000000000002 No Hit ATTCGACATTCAGGATATGAACTTTATTGCCGCCATTTTAAATAAGCACC 7 0.17500000000000002 No Hit GTTACGAGGAAATGGTTGAATTCATGGAGAAGGTGGCCAAGACTGCCGAT 7 0.17500000000000002 No Hit CATGGCCAAGCTCGCCGAGCAGGCTGAGCGTTACGAGGAAATGGTTGAAT 7 0.17500000000000002 No Hit CTTTACCACACATAAACTGCCTCATGACTTCCCGGTTCACATTAATTGAC 7 0.17500000000000002 No Hit CAATCCCACGAGCTGAGCTTCGCGTTGCGTTTCTCTCTCGCGTCATCTCT 7 0.17500000000000002 No Hit TGTTGCTTGTGGGATCATCGGGCTCCAGGGCTAGATGGGGTAATCGCTTG 7 0.17500000000000002 No Hit ATAATTTATGAGCGTGGCTTGTTTGGAATGTATCATGATTGGTGTGAATC 7 0.17500000000000002 No Hit ACTGTTGAGGAGCGTAACCTACTTTCTGTGGCTTATAAGAACGTGATTGG 7 0.17500000000000002 No Hit ATGTACTTGAAGGAAGTGCAGGCACACCCTTTGAAGGCGGATTCTACTAT 7 0.17500000000000002 No Hit GGCGGTTTTGCTGCAGCTCTGAGGGGCCAGCGACAAGTCTGGGTTATGAA 7 0.17500000000000002 No Hit GTGAGCTCACTGTTGAGGAGCGTAACCTACTTTCTGTGGCTTATAAGAAC 7 0.17500000000000002 No Hit AAGACTGCCGATGTCGGTGAGCTCACTGTTGAGGAGCGTAACCTACTTTC 6 0.15 No Hit AAAATGTCTACTGCTGAGGCAACCCGTGAGGAGAATGTCTACATGGCCAA 6 0.15 No Hit ATCAGCTAAGGCTTTAGAGTTAATGAAGGAAAATGAGAAGGCAACAACTC 6 0.15 No Hit AGAACGTGATTGGTGCCCGGAGGGCATCATGGAGGATCATCTCATCCATT 6 0.15 No Hit CGTGAGGAGAATGTCTACATGGCCAAGCTCGCCGAGCAGGCTGAGCGTTA 6 0.15 No Hit AGGAAATGGTTGAATTCATGGAGAAGGTGGCCAAGACTGCCGATGTCGGT 6 0.15 No Hit GCACTTGGTTCTGCCTGTTCCTGCATTCAACGTCATTAACGGTGGATCCC 6 0.15 No Hit GTGGCCAAGACTGCCGATGTCGGTGAGCTCACTGTTGAGGAGCGTAACCT 6 0.15 No Hit CACGCGCTCTGCAAGGAGCCGCCGCCGCAGATCGTGGCGCGCCCGCTGCC 6 0.15 No Hit GAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAGCAAGTTCGTGTAACA 6 0.15 No Hit GGCCAAGCTCGCCGAGCAGGCTGAGCGTTACGAGGAAATGGTTGAATTCA 6 0.15 No Hit GCAACCCGTGAGGAGAATGTCTACATGGCCAAGCTCGCCGAGCAGGCTGA 6 0.15 No Hit GATCGCTGATCAGTGTGCACCATTCACATCATTTCCTATGTGGCTGTTGG 6 0.15 No Hit GGTCCGACCACCGTGACAGGAAGTGTCTCTGGACTCAAGGAAGGGCTCCA 6 0.15 No Hit CTTGGAAATGTGACGGCTGGAGTGGATGGTGTTGCTAACATCAATGTTGT 6 0.15 No Hit GCACATTCGACATTCAGGATATGAACTTTATTGCCGCCATTTTAAATAAG 6 0.15 No Hit GTTCTTGACATGACTGGTTTGAAGTTGTCACAACTGAGCCAAATGAAGAT 6 0.15 No Hit GCTGTTTGCAAAGCCGGAGCTTCCGTGAAGAAGATTCCGCTGTACCAGCA 6 0.15 No Hit GTAATCGCTTGCCAACACCGTACAGATCTGGGCACATTCGACATTCAGGA 6 0.15 No Hit GAGCAGGCTGAGCGTTACGAGGAAATGGTTGAATTCATGGAGAAGGTGGC 6 0.15 No Hit GTTGGAACATTTGCATCACCATCATTTGCCTTTTGAGTGACTGTTCTGTC 6 0.15 No Hit GGTGGGTCTGACTACTTGGGCAAGGGTGTTTCCAAGGCTGTTAACAATGT 6 0.15 No Hit GCAGCGCACACACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTT 6 0.15 No Hit GGTAATCGCTTGCCAACACCGTACAGATCTGGGCACATTCGACATTCAGG 6 0.15 No Hit AGATACTGACGTCCAGTATGCATTGACATGTATCGAGAAAGTTGTCGAAG 6 0.15 No Hit TCGGGCTCCAGGGCTAGATGGGGTAATCGCTTGCCAACACCGTACAGATC 6 0.15 No Hit GTTTCCAAGGCTGTTAACAATGTGAACTCTATTATTGCACCAGCTTTGAT 6 0.15 No Hit CGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCGAGCAAGTTCGTGTA 6 0.15 No Hit CGACATTCAGGATATGAACTTTATTGCCGCCATTTTAAATAAGCACCTGA 6 0.15 No Hit GCTGAGCGTTACGAGGAAATGGTTGAATTCATGGAGAAGGTGGCCAAGAC 6 0.15 No Hit ATAAGAACGTGATTGGTGCCCGGAGGGCATCATGGAGGATCATCTCATCC 6 0.15 No Hit CCTCTCTCTATTCTGACTACTCCTGACTGCTGGAGTTTCCTTCACAAATG 6 0.15 No Hit CTTAGCAGCAAAACAAAGCAAATTAGTAACAAAAGAAACCTCCTCCACAT 6 0.15 No Hit CGTACAGATCTGGGCACATTCGACATTCAGGATATGAACTTTATTGCCGC 6 0.15 No Hit GTTCCAAGCGCCGGAGGAAAGGTACCGAGCAAGTTCGTGTAACAAAGGAG 6 0.15 No Hit CCCACGAACCTGCAGCGCACACACGAGATCCGCAGCCGAGAGGGGAGCCC 6 0.15 No Hit CCAAGTATCAGCATGACAACCCCCAGCGGCAGGTTTGCCCCTCACAAAAG 6 0.15 No Hit CACACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGC 6 0.15 No Hit ATTTTAAATAAGCACCTGATCCATGATCGCTGATCAGTGTGCACCATTCA 6 0.15 No Hit CACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCG 6 0.15 No Hit GTGACTTTCATCCAGAATCTTGGAATCCAATGTGGTCTGTAGCAAGCATT 6 0.15 No Hit TGGGAATGCTGGTGCACGTGTTGCTTGTGGGATCATCGGGCTCCAGGGCT 6 0.15 No Hit GTTTCTGATACCACTTGTAGGAAAGCTTCATCATTAGGTAGCACATGAGT 6 0.15 No Hit GCGGATTCTACTATGGGAAGCTCAAATTCCCATCCGATTATCCTTTTAAA 6 0.15 No Hit GCACACACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCAAG 6 0.15 No Hit ATTGGAGTGGCACTATGTACTTGAAGGAAGTGCAGGCACACCCTTTGAAG 6 0.15 No Hit GCCCGGAGGGCATCATGGAGGATCATCTCATCCATTGAGCAGAAGGAGGA 6 0.15 No Hit AGTTAATGAAGGAAAATGAGAAGGCAACAACTCCACCGGCGCAACAAAAG 5 0.125 No Hit TGATCGCTGATCAGTGTGCACCATTCACATCATTTCCTATGTGGCTGTTG 5 0.125 No Hit GATGGGGTAATCGCTTGCCAACACCGTACAGATCTGGGCACATTCGACAT 5 0.125 No Hit CGTGATTGGTGCCCGGAGGGCATCATGGAGGATCATCTCATCCATTGAGC 5 0.125 No Hit TTTTAAACCTCCAAGTATCAGCATGACAACCCCCAGCGGCAGGTTTGCCC 5 0.125 No Hit CAACCCGTGAGGAGAATGTCTACATGGCCAAGCTCGCCGAGCAGGCTGAG 5 0.125 No Hit GATTGGTGCCCGGAGGGCATCATGGAGGATCATCTCATCCATTGAGCAGA 5 0.125 No Hit GTGATTGGTGCCCGGAGGGCATCATGGAGGATCATCTCATCCATTGAGCA 5 0.125 No Hit CTACCATCCGATACTCACAGCGAGACGACATCAGAACCAAAATCTGCAGG 5 0.125 No Hit ACCCTATTTTGCCATCAAAGATTATAGCACCATTTTCATCATACCTAGGT 5 0.125 No Hit ATCATTTCCTATGTGGCTGTTGGAACATTTGCATCACCATCATTTGCCTT 5 0.125 No Hit GCCTGAAGCGCCTCCAGAAGGAGTATCACGCGCTCTGCAAGGAGCCGCCG 5 0.125 No Hit ACCTGATCCATGATCGCTGATCAGTGTGCACCATTCACATCATTTCCTAT 5 0.125 No Hit CAATATCTTCATCAGGCCACATGTTCTGTATAGCTGGAACAACTTTACCA 5 0.125 No Hit ATGTCCCCCTCTCTCTATTCTGACTACTCCTGACTGCTGGAGTTTCCTTC 5 0.125 No Hit GAGGAAATGGTTGAATTCATGGAGAAGGTGGCCAAGACTGCCGATGTCGG 5 0.125 No Hit GGCGGATTGAGGGTTGCCGATCGAGGGGCGCGCGTGCGGGGGGATTTGAG 5 0.125 No Hit CATCGGGCTCCAGGGCTAGATGGGGTAATCGCTTGCCAACACCGTACAGA 5 0.125 No Hit GTTGGGATGAGCTGATGGATAAGCTTTTCAGCAGGGATGAAGATGGTAAG 5 0.125 No Hit CATAAATTGCCTGTTGGTCCATCAGTGAGGGGGTCAAAATGGCCAGAGTT 5 0.125 No Hit ATCACCCCCTTGCTTTTTCTTGCCTACTCTTTCTTTCCCACAATCCCACG 5 0.125 No Hit TGTACTTGAAGGAAGTGCAGGCACACCCTTTGAAGGCGGATTCTACTATG 5 0.125 No Hit GCTTGATGGAACCAAAAACGAGTGGGGTTGGTGCAAGCAGAAGCTTGGTG 5 0.125 No Hit GGTACCGAGCAAGTTCGTGTAACAAAGGAGAGGAAAAATGTCTACTGCTG 5 0.125 No Hit ATCCTGATGATCTTGGCAAGGGTGGACATGAGCTTAGCAAGAGCACTGGG 5 0.125 No Hit GATCATCGGGCTCCAGGGCTAGATGGGGTAATCGCTTGCCAACACCGTAC 5 0.125 No Hit ATGAGCTTAGCAAGAGCACTGGGAATGCTGGTGCACGTGTTGCTTGTGGG 5 0.125 No Hit CATTAATTGACTTAATTTCTAATGTCCCCCTCTCTCTATTCTGACTACTC 5 0.125 No Hit CAGTGCTAAAGTGTTCTCGTACAGTTCAGCGTCACAATCTAGAGAAGCAG 5 0.125 No Hit GGATATGAACTTTATTGCCGCCATTTTAAATAAGCACCTGATCCATGATC 5 0.125 No Hit GTGCAAGCAGAAGCTTGGTGCTAATGCAATCCTGGCTGTGTCACTAGCTG 5 0.125 No Hit GGCTAGATGGGGTAATCGCTTGCCAACACCGTACAGATCTGGGCACATTC 5 0.125 No Hit ATCGGGCTCCAGGGCTAGATGGGGTAATCGCTTGCCAACACCGTACAGAT 5 0.125 No Hit AAGCACCTGATCCATGATCGCTGATCAGTGTGCACCATTCACATCATTTC 5 0.125 No Hit CACCAATGGCTGCATGTCAACTGGACCACACTTTAACCCCGCTGGTAAGG 5 0.125 No Hit CCTATTTTGCCATCAAAGATTATAGCACCATTTTCATCATACCTAGGTCT 5 0.125 No Hit CGCTGATCAGTGTGCACCATTCACATCATTTCCTATGTGGCTGTTGGAAC 5 0.125 No Hit GTGAACTCTATTATTGCACCAGCTTTGATTGGCAAGGACCCTACCACACA 5 0.125 No Hit AAAAATGTCTACTGCTGAGGCAACCCGTGAGGAGAATGTCTACATGGCCA 5 0.125 No Hit GCGCACACACGAGATCCGCAGCCGAGAGGGGAGCCCGTGAGGAAGTTCCA 5 0.125 No Hit ACGCGGCCGCCGATCCCCATCCCCTTTCCCTCCTCTCCGGTTCACCCCAA 5 0.125 No Hit CAAAGGAGAGGAAAAATGTCTACTGCTGAGGCAACCCGTGAGGAGAATGT 5 0.125 No Hit CTTCATCAGGCCACATGTTCTGTATAGCTGGAACAACTTTACCACACATA 5 0.125 No Hit GCTGGTGCACGTGTTGCTTGTGGGATCATCGGGCTCCAGGGCTAGATGGG 5 0.125 No Hit AACTCTATTATTGCACCAGCTTTGATTGGCAAGGACCCTACCACACAAGC 5 0.125 No Hit AATCCCACGAGCTGAGCTTCGCGTTGCGTTTCTCTCTCGCGTCATCTCTC 5 0.125 No Hit TGGTGATCCTGATGATCTTGGCAAGGGTGGACATGAGCTTAGCAAGAGCA 5 0.125 No Hit TATAAGAACGTGATTGGTGCCCGGAGGGCATCATGGAGGATCATCTCATC 5 0.125 No Hit CCGAGAGGGGAGCCCGTGAGGAAGTTCCAAGCGCCGGAGGAAAGGTACCG 5 0.125 No Hit GGGTGGACATGAGCTTAGCAAGAGCACTGGGAATGCTGGTGCACGTGTTG 5 0.125 No Hit GGAGCGTAACCTACTTTCTGTGGCTTATAAGAACGTGATTGGTGCCCGGA 5 0.125 No Hit GCAAGCAGAAGCTTGGTGCTAATGCAATCCTGGCTGTGTCACTAGCTGTT 5 0.125 No Hit ATTTCCTATGTGGCTGTTGGAACATTTGCATCACCATCATTTGCCTTTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.025 0.0 0.0 0.0 0.0 124-125 0.025 0.0 0.0 0.0 0.0 126-127 0.025 0.0 0.0 0.0 0.0 128-129 0.025 0.0 0.0 0.0 0.0 130-131 0.025 0.0 0.0 0.0 0.0 132-133 0.025 0.0 0.0 0.0 0.0 134-135 0.025 0.0 0.0 0.0 0.0 136-137 0.025 0.0 0.0 0.0 0.0 138 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCAGA 10 0.007991117 137.58751 4 >>END_MODULE Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487758 spots for ERR5262804.sra Written 1487758 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra Read 1487748 spots for ERR5262804.sra Written 1487748 spots for ERR5262804.sra SRR ids: ['ERR5262804.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_09eyn_b2 ERR5262804.sra spots: 29754970 blocks: [[1, 1487748], [1487749, 2975496], [2975497, 4463244], [4463245, 5950992], [5950993, 7438740], [7438741, 8926488], [8926489, 10414236], [10414237, 11901984], [11901985, 13389732], [13389733, 14877480], [14877481, 16365228], [16365229, 17852976], [17852977, 19340724], [19340725, 20828472], [20828473, 22316220], [22316221, 23803968], [23803969, 25291716], [25291717, 26779464], [26779465, 28267212], [28267213, 29754970]] ERR5262804 file size 9816158 ERR5262804 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262804 ERR5262804_1.fastq ERR5262804_2.fastq Input file: ERR5262804_1.fastq Paired file: ERR5262804_2.fastq trimmed: ERR5262804-trimmed-pair1.fastq, ERR5262804-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:11:12 2024 >> started Fri Dec 6 12:11:41 2024 >> done (29.528s) 29754970 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 29754969 (100.00%) read pairs available; of these: 7193 ( 0.02%) trimmed read pairs available after processing 29747776 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 1 0.00% 23 1 0.00% 24 1 0.00% 25 1 0.00% 26 3 0.00% 27 5 0.00% 28 7 0.00% 29 3 0.00% 30 2 0.00% 31 3 0.00% 32 6 0.00% 33 3 0.00% 34 4 0.00% 35 5 0.00% 36 7 0.00% 37 5 0.00% 38 6 0.00% 39 9 0.00% 40 5 0.00% 41 2 0.00% 42 9 0.00% 43 5 0.00% 44 2 0.00% 45 4 0.00% 46 2 0.00% 47 7 0.00% 48 1 0.00% 49 259 0.00% 50 255 0.00% 51 262 0.00% 52 316 0.00% 53 329 0.00% 54 351 0.00% 55 378 0.00% 56 433 0.00% 57 471 0.00% 58 491 0.00% 59 620 0.00% 60 651 0.00% 61 756 0.00% 62 877 0.00% 63 926 0.00% 64 989 0.00% 65 1046 0.00% 66 1203 0.00% 67 1544 0.01% 68 1626 0.01% 69 1924 0.01% 70 2116 0.01% 71 2439 0.01% 72 2730 0.01% 73 3297 0.01% 74 3368 0.01% 75 3700 0.01% 76 4279 0.01% 77 4756 0.02% 78 5179 0.02% 79 5699 0.02% 80 6492 0.02% 81 7177 0.02% 82 8400 0.03% 83 9205 0.03% 84 9981 0.03% 85 11189 0.04% 86 11907 0.04% 87 12946 0.04% 88 13808 0.05% 89 14755 0.05% 90 16383 0.06% 91 17479 0.06% 92 18750 0.06% 93 20620 0.07% 94 22345 0.08% 95 23467 0.08% 96 24928 0.08% 97 26549 0.09% 98 27380 0.09% 99 28490 0.10% 100 30157 0.10% 101 31285 0.11% 102 33300 0.11% 103 35599 0.12% 104 37269 0.13% 105 39102 0.13% 106 40401 0.14% 107 41829 0.14% 108 43050 0.14% 109 44755 0.15% 110 46027 0.15% 111 47944 0.16% 112 49823 0.17% 113 51204 0.17% 114 53644 0.18% 115 55916 0.19% 116 57190 0.19% 117 59440 0.20% 118 60298 0.20% 119 61456 0.21% 120 62632 0.21% 121 63756 0.21% 122 65008 0.22% 123 66404 0.22% 124 69687 0.23% 125 70673 0.24% 126 72605 0.24% 127 73499 0.25% 128 75256 0.25% 129 76631 0.26% 130 76492 0.26% 131 77759 0.26% 132 79613 0.27% 133 80758 0.27% 134 83390 0.28% 135 85343 0.29% 136 86389 0.29% 137 87761 0.29% 138 88819 0.30% 139 91312 0.31% 140 91151 0.31% 141 94264 0.32% 142 97221 0.33% 143 97670 0.33% 144 100461 0.34% 145 99649 0.33% 146 103755 0.35% 147 247370 0.83% 148 96551 0.32% 149 96872 0.33% 150 25790898 86.68% 29754969 reads passed initial QC criterion=sequence-density sequence-density=2.62 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=31 prefix-density=2.84 prefix-fanout=2.1 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.05 sequence-density-rank=25 fanout-score=176.19 fanout-score-rank=1 prefix-density=1.42 prefix-fanout=6.1 sequence=CCGCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=10.07 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=30 prefix-density=10.04 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.21 sequence-density-rank=24 fanout-score=103.39 fanout-score-rank=1 prefix-density=10.24 prefix-fanout=2.1 sequence=GTTCCGGTTCGCGGCTAGCAGTAGTTGTTGTAGTAGCAGCTAGGGTTTCCGGTAGGGTTCCGTCGAGATCGCCATGGATGAGTACCGCTGCTTCGTGGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262804 ERR5262804_1.fastq ERR5262804_2.fastq Input file: ERR5262804_1.fastq Paired file: ERR5262804_2.fastq trimmed: ERR5262804-trimmed-pair1.fastq, ERR5262804-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:14:23 2024 >> started Fri Dec 6 12:14:43 2024 >> done (20.133s) 21253549 read pairs processed; of these: 104 ( 0.00%) short read pairs filtered out after trimming by size control 443 ( 0.00%) empty read pairs filtered out after trimming by size control 21253002 (100.00%) read pairs available; of these: 124 ( 0.00%) trimmed read pairs available after processing 21252878 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 1 0.00% 23 0 0.00% 24 1 0.00% 25 1 0.00% 26 2 0.00% 27 5 0.00% 28 7 0.00% 29 1 0.00% 30 2 0.00% 31 2 0.00% 32 4 0.00% 33 2 0.00% 34 3 0.00% 35 3 0.00% 36 5 0.00% 37 2 0.00% 38 2 0.00% 39 8 0.00% 40 5 0.00% 41 2 0.00% 42 5 0.00% 43 4 0.00% 44 2 0.00% 45 3 0.00% 46 1 0.00% 47 6 0.00% 48 1 0.00% 49 234 0.00% 50 219 0.00% 51 236 0.00% 52 288 0.00% 53 293 0.00% 54 311 0.00% 55 330 0.00% 56 179 0.00% 57 328 0.00% 58 129 0.00% 59 185 0.00% 60 181 0.00% 61 517 0.00% 62 805 0.00% 63 840 0.00% 64 916 0.00% 65 947 0.00% 66 1115 0.01% 67 598 0.00% 68 774 0.00% 69 1718 0.01% 70 1059 0.00% 71 2091 0.01% 72 2463 0.01% 73 1445 0.01% 74 3063 0.01% 75 2095 0.01% 76 2197 0.01% 77 4224 0.02% 78 3204 0.02% 79 4642 0.02% 80 4386 0.02% 81 5010 0.02% 82 7469 0.04% 83 5806 0.03% 84 7256 0.03% 85 7071 0.03% 86 7849 0.04% 87 10181 0.05% 88 10172 0.05% 89 10368 0.05% 90 11744 0.06% 91 12149 0.06% 92 13863 0.07% 93 14002 0.07% 94 15720 0.07% 95 18235 0.09% 96 16752 0.08% 97 18747 0.09% 98 20031 0.09% 99 19492 0.09% 100 21902 0.10% 101 21999 0.10% 102 24351 0.11% 103 24795 0.12% 104 27227 0.13% 105 27000 0.13% 106 29760 0.14% 107 29084 0.14% 108 30972 0.15% 109 30862 0.15% 110 33113 0.16% 111 34492 0.16% 112 34766 0.16% 113 36585 0.17% 114 38827 0.18% 115 39154 0.18% 116 40567 0.19% 117 41617 0.20% 118 43397 0.20% 119 44384 0.21% 120 43689 0.21% 121 46067 0.22% 122 45983 0.22% 123 47432 0.22% 124 49641 0.23% 125 50333 0.24% 126 51643 0.24% 127 52180 0.25% 128 53412 0.25% 129 54040 0.25% 130 54388 0.26% 131 55168 0.26% 132 56685 0.27% 133 57317 0.27% 134 59428 0.28% 135 60842 0.29% 136 61227 0.29% 137 62612 0.29% 138 62827 0.30% 139 64965 0.31% 140 64867 0.31% 141 67163 0.32% 142 69079 0.33% 143 70199 0.33% 144 71546 0.34% 145 70849 0.33% 146 74023 0.35% 147 175921 0.83% 148 69024 0.32% 149 69012 0.32% 150 18432576 86.73% criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=4.48 fanout-score-rank=37 prefix-density=0.34 prefix-fanout=3.7 sequence=TGCAGTTGTCGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=36 fanout-score=679.38 fanout-score-rank=1 prefix-density=0.69 prefix-fanout=19.5 sequence=CGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTG criterion=sequence-density sequence-density=0.89 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=39 prefix-density=0.89 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=721.00 fanout-score-rank=1 prefix-density=1.09 prefix-fanout=15.5 sequence=GCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTACGCGTGGGTGCTTGACAAGCTGAAGGCTGAGCGTGAGAGAGGTATCACCATCGATATTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGATGCCCCTGGACACCGTGACTTCATCAAGAACATGATTACCGGTACCTCCCAGGCTGACTGTGCCGTGCTTATCATTGACTCCACGACTGGAGGTTTTGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCATGCCCTCCTTGCTTTCACTCTTGGAGTGAAGCAGATGATCTGCTGCTGCAACAAGATGGA ERR5262804 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:16:01 Started mapping on | Dec 06 12:16:01 Finished on | Dec 06 12:18:55 Mapping speed, Million of reads per hour | 615.61 Number of input reads | 29754422 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 28438964 Uniquely mapped reads % | 95.58% Average mapped length | 292.54 Number of splices: Total | 28253938 Number of splices: Annotated (sjdb) | 26281427 Number of splices: GT/AG | 27841588 Number of splices: GC/AG | 345128 Number of splices: AT/AC | 18520 Number of splices: Non-canonical | 48702 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.35 Insertion rate per base | 0.01% Insertion average length | 2.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 323429 % of reads mapped to multiple loci | 1.09% Number of reads mapped to too many loci | 678 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.32% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 992029 992029 992029 N_multimapping 323429 323429 323429 N_noFeature 1028045 27702999 1256414 N_ambiguous 597428 4046 90248 UnstrandedReadsAssigned:26813491 PositiveStrandReadsAssigned:731919 NegativeStrandReadsAssigned:27092302 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262804 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262804-trimmed-pair1.fastq ERR5262804-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,754,422 reads, 27,418,203 reads pseudoaligned [quant] estimated average fragment length: 277.901 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,412 rounds 52973 ERR5262804.ke.tsv 35125 ERR5262804.se.tsv 88098 total ==> ERR5262804.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 659.695 0 0 PNS24247 1044 767.099 113.837 7.58391 PNS24249 1928 1651.1 341.366 10.5659 PNS24246 1044 767.099 113.837 7.58391 PNS24248 1044 767.099 113.837 7.58391 PNS24244 1471 1194.1 128.123 5.48336 PNS24243 293 101.034 0 0 KQK14069 1603 1326.1 60279.7 2323.04 KQK14071 474 231.759 610.949 134.719 ==> ERR5262804.se.tsv <== BRADI_1g14170v3 63205 BRADI_1g53295v3 293 BRADI_1g59795v3 822 BRADI_1g07683v3 0 BRADI_1g00485v3 10 BRADI_1g20270v3 933 BRADI_1g74790v3 2756 BRADI_1g09890v3 1 BRADI_1g77505v3 410 BRADI_1g48960v3 0 ERR5262804 completed mapping pipeline successfully