Starting /dee2/code/volunteer_pipeline.sh ERR5262805 current disk space = 1551426678784 free memory = 1368225848 ERR5262805 SRAfilesize c2916727ec72f5b849d4b3660015798b ERR5262805.sra ERR5262805.sra file validated ERR5262805 is paired end ERR5262805 is conventional basespace ERR5262805 read1 length is 79-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262805_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 79-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4945 37.0 37.0 37.0 37.0 37.0 2 36.5785 37.0 37.0 37.0 37.0 37.0 3 36.6645 37.0 37.0 37.0 37.0 37.0 4 36.7085 37.0 37.0 37.0 37.0 37.0 5 36.645 37.0 37.0 37.0 37.0 37.0 6 36.5045 37.0 37.0 37.0 37.0 37.0 7 36.5775 37.0 37.0 37.0 37.0 37.0 8 36.658 37.0 37.0 37.0 37.0 37.0 9 36.6965 37.0 37.0 37.0 37.0 37.0 10-14 36.63119999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.59889999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.461200000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.4752 37.0 37.0 37.0 37.0 37.0 30-34 36.430600000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.4299 37.0 37.0 37.0 37.0 37.0 40-44 36.1668 37.0 37.0 37.0 37.0 37.0 45-49 36.3216 37.0 37.0 37.0 37.0 37.0 50-54 36.4816 37.0 37.0 37.0 37.0 37.0 55-59 36.493100000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.484899999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.43770000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.511 37.0 37.0 37.0 37.0 37.0 75-79 36.457100000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.46280495567975 37.0 37.0 37.0 37.0 37.0 85-89 36.44725526126577 37.0 37.0 37.0 37.0 37.0 90-94 36.3248048782783 37.0 37.0 37.0 37.0 37.0 95-99 36.35178988015276 37.0 37.0 37.0 37.0 37.0 100-104 36.36033149810219 37.0 37.0 37.0 37.0 37.0 105-109 36.31186037837606 37.0 37.0 37.0 37.0 37.0 110-114 36.111432208855064 37.0 37.0 37.0 37.0 37.0 115-119 36.24838461573053 37.0 37.0 37.0 37.0 37.0 120-124 36.15349201296213 37.0 37.0 37.0 37.0 37.0 125-129 36.12464093462573 37.0 37.0 37.0 37.0 37.0 130-134 36.18354422925411 37.0 37.0 37.0 37.0 37.0 135-139 36.04621718385836 37.0 37.0 37.0 37.0 37.0 140-144 35.98534370540731 37.0 37.0 37.0 37.0 37.0 145-149 36.00145299532618 37.0 37.0 37.0 37.0 37.0 150 35.94303440496334 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1104 1 0.3571428571428541 1104 2 0.24223602484472195 1104 3 0.48136645962733127 1104 4 0.29813664596273526 1104 5 0.30175983436853215 1104 6 0.4704968944099406 1104 7 0.366459627329192 1104 8 0.40062111801242395 1104 9 0.08281573498964434 1104 10-14 0.3698757763974996 1104 15-19 0.4339544513457483 1104 20-24 0.42826086956520726 1104 25-29 0.4900621118012225 1104 30-34 0.41428571428571104 1104 35-39 0.5534161490683118 1104 40-44 0.8229813664595866 1104 45-49 0.6233954451345625 1104 50-54 0.4873706004140743 1104 55-59 0.44637681159419174 1104 60-64 0.44720496894409223 1104 65-69 0.47670807453415165 1104 70-74 0.40890269151137204 1104 75-79 0.5108695652173836 1104 80-84 0.4166666666666572 1104 85-89 0.5655279503105461 1104 90-94 0.7276397515527862 1104 95-99 0.6453416149068261 1104 100-104 0.6684265010351851 1104 105-109 0.7360248447204754 1104 110-114 1.1671842650103343 1104 115-119 0.9860248447204967 1104 120-124 1.2381987577639677 1104 125-129 1.6865424430641625 1104 130-134 1.9863354037267058 1104 135-139 2.98944099378879 1104 140-144 3.367494824016539 1104 145-149 -1.2513457556935599 1104 150 4.965838509316768 1105 1 0.3571428571428541 1105 2 0.24223602484472195 1105 3 0.48136645962733127 1105 4 0.29813664596273526 1105 5 0.30175983436853215 1105 6 0.4704968944099406 1105 7 0.366459627329192 1105 8 0.40062111801242395 1105 9 0.08281573498964434 1105 10-14 0.3698757763974996 1105 15-19 0.4339544513457483 1105 20-24 0.42826086956520726 1105 25-29 0.4900621118012225 1105 30-34 0.41428571428571104 1105 35-39 0.5534161490683118 1105 40-44 0.8229813664595866 1105 45-49 0.6233954451345625 1105 50-54 0.4873706004140743 1105 55-59 0.44637681159419174 1105 60-64 0.44720496894409223 1105 65-69 0.47670807453415165 1105 70-74 0.40890269151137204 1105 75-79 0.5108695652173836 1105 80-84 0.4166666666666572 1105 85-89 0.5655279503105461 1105 90-94 0.7276397515527862 1105 95-99 0.6453416149068261 1105 100-104 0.6684265010351851 1105 105-109 0.7360248447204754 1105 110-114 1.1671842650103343 1105 115-119 0.9860248447204967 1105 120-124 1.2381987577639677 1105 125-129 1.6865424430641625 1105 130-134 -0.4136645962732928 1105 135-139 -2.2105590062112093 1105 140-144 3.367494824016539 1105 145-149 3.9486542443064394 1105 150 -21.034161490683232 1107 1 0.3571428571428541 1107 2 0.24223602484472195 1107 3 0.48136645962733127 1107 4 0.29813664596273526 1107 5 0.30175983436853215 1107 6 0.4704968944099406 1107 7 0.366459627329192 1107 8 0.40062111801242395 1107 9 0.08281573498964434 1107 10-14 -0.8301242236025033 1107 15-19 0.4339544513457483 1107 20-24 0.42826086956520726 1107 25-29 0.4900621118012225 1107 30-34 0.41428571428571104 1107 35-39 -0.646583850931691 1107 40-44 -1.7770186335404148 1107 45-49 0.6233954451345625 1107 50-54 0.4873706004140743 1107 55-59 0.44637681159419174 1107 60-64 0.44720496894409223 1107 65-69 0.47670807453415165 1107 70-74 0.40890269151137204 1107 75-79 0.5108695652173836 1107 80-84 0.4166666666666572 1107 85-89 0.5655279503105461 1107 90-94 0.7276397515527862 1107 95-99 0.6453416149068261 1107 100-104 0.6684265010351851 1107 105-109 0.7360248447204754 1107 110-114 1.1671842650103343 1107 115-119 0.9860248447204967 1107 120-124 1.2381987577639677 1107 125-129 1.6865424430641625 1107 130-134 1.9863354037267058 1107 135-139 2.98944099378879 1107 140-144 3.367494824016539 1107 145-149 3.9486542443064394 1107 150 4.965838509316768 1109 1 0.3571428571428541 1109 2 0.24223602484472195 1109 3 0.48136645962733127 1109 4 0.29813664596273526 1109 5 0.30175983436853215 1109 6 0.4704968944099406 1109 7 0.366459627329192 1109 8 0.40062111801242395 1109 9 0.08281573498964434 1109 10-14 0.3698757763974996 1109 15-19 0.4339544513457483 1109 20-24 0.42826086956520726 1109 25-29 0.4900621118012225 1109 30-34 0.41428571428571104 1109 35-39 0.5534161490683118 1109 40-44 -9.177018633540413 1109 45-49 0.6233954451345625 1109 50-54 -1.9126293995859243 1109 55-59 0.44637681159419174 1109 60-64 0.44720496894409223 1109 65-69 -1.923291925465847 1109 70-74 0.40890269151137204 1109 75-79 0.5108695652173836 1109 80-84 0.4166666666666572 1109 85-89 0.5655279503105461 1109 90-94 0.7276397515527862 1109 95-99 -1.7546583850931725 1109 100-104 0.6684265010351851 1109 105-109 0.7360248447204754 1109 110-114 -1.2328157349896642 1109 115-119 0.9860248447204967 1109 120-124 1.2381987577639677 1109 125-129 1.6865424430641625 1109 130-134 1.9863354037267058 1109 135-139 0.5894409937887914 1109 140-144 0.9674948240165406 1109 145-149 3.9486542443064394 1109 150 4.965838509316768 1111 1 0.3571428571428541 1111 2 0.24223602484472195 1111 3 0.48136645962733127 1111 4 0.29813664596273526 1111 5 0.30175983436853215 1111 6 0.4704968944099406 1111 7 0.366459627329192 1111 8 0.40062111801242395 1111 9 0.08281573498964434 1111 10-14 0.3698757763974996 1111 15-19 0.4339544513457483 1111 20-24 0.42826086956520726 1111 25-29 -1.909937888198776 1111 30-34 0.41428571428571104 1111 35-39 0.5534161490683118 1111 40-44 0.8229813664595866 1111 45-49 0.6233954451345625 1111 50-54 0.4873706004140743 1111 55-59 0.44637681159419174 1111 60-64 0.44720496894409223 1111 65-69 0.47670807453415165 1111 70-74 0.40890269151137204 1111 75-79 0.5108695652173836 1111 80-84 0.4166666666666572 1111 85-89 0.5655279503105461 1111 90-94 0.7276397515527862 1111 95-99 0.6453416149068261 1111 100-104 0.6684265010351851 1111 105-109 0.7360248447204754 1111 110-114 1.1671842650103343 1111 115-119 0.9860248447204967 1111 120-124 1.2381987577639677 1111 125-129 1.6865424430641625 1111 130-134 1.9863354037267058 1111 135-139 2.98944099378879 1111 140-144 0.9674948240165406 1111 145-149 3.9486542443064394 1111 150 -32.03416149068323 1112 1 0.3571428571428541 1112 2 0.24223602484472195 1112 3 0.48136645962733127 1112 4 0.29813664596273526 1112 5 0.30175983436853215 1112 6 0.4704968944099406 1112 7 0.366459627329192 1112 8 0.40062111801242395 1112 9 0.08281573498964434 1112 10-14 0.3698757763974996 1112 15-19 0.4339544513457483 1112 20-24 0.42826086956520726 1112 25-29 0.4900621118012225 1112 30-34 0.41428571428571104 1112 35-39 0.5534161490683118 1112 40-44 0.8229813664595866 1112 45-49 0.6233954451345625 1112 50-54 0.4873706004140743 1112 55-59 0.44637681159419174 1112 60-64 0.44720496894409223 1112 65-69 0.47670807453415165 1112 70-74 0.40890269151137204 1112 75-79 0.5108695652173836 1112 80-84 0.4166666666666572 1112 85-89 0.5655279503105461 1112 90-94 0.7276397515527862 1112 95-99 0.6453416149068261 1112 100-104 0.6684265010351851 1112 105-109 0.7360248447204754 1112 110-114 1.1671842650103343 1112 115-119 0.9860248447204967 1112 120-124 1.2381987577639677 1112 125-129 1.6865424430641625 1112 130-134 1.9863354037267058 1112 135-139 0.5894409937887914 1112 140-144 3.367494824016539 1112 145-149 -33.05134575569356 1112 150 -32.03416149068323 1114 1 0.3571428571428541 1114 2 0.24223602484472195 1114 3 0.48136645962733127 1114 4 0.29813664596273526 1114 5 0.30175983436853215 1114 6 0.4704968944099406 1114 7 0.366459627329192 1114 8 0.40062111801242395 1114 9 0.08281573498964434 1114 10-14 0.3698757763974996 1114 15-19 0.4339544513457483 1114 20-24 0.42826086956520726 1114 25-29 -0.7099378881987803 1114 30-34 0.41428571428571104 1114 35-39 0.5534161490683118 1114 40-44 -1.577018633540412 1114 45-49 -0.5766045548654404 1114 50-54 -0.7126293995859285 1114 55-59 0.44637681159419174 1114 60-64 0.44720496894409223 1114 65-69 0.47670807453415165 1114 70-74 0.40890269151137204 1114 75-79 0.5108695652173836 1114 80-84 -0.7833333333333456 1114 85-89 0.5655279503105461 1114 90-94 0.7276397515527862 1114 95-99 -1.9546583850931754 1114 100-104 0.6684265010351851 1114 105-109 0.7360248447204754 1114 110-114 1.1671842650103343 1114 115-119 0.9860248447204967 1114 120-124 1.2381987577639677 1114 125-129 -0.913457556935839 1114 130-134 1.9863354037267058 1114 135-139 2.98944099378879 1114 140-144 3.367494824016539 1114 145-149 3.9486542443064394 1114 150 4.965838509316768 1121 1 0.3571428571428541 1121 2 0.24223602484472195 1121 3 0.48136645962733127 1121 4 0.29813664596273526 1121 5 0.30175983436853215 1121 6 0.4704968944099406 1121 7 0.366459627329192 1121 8 0.40062111801242395 1121 9 0.08281573498964434 1121 10-14 0.3698757763974996 1121 15-19 0.4339544513457483 1121 20-24 0.42826086956520726 1121 25-29 0.4900621118012225 1121 30-34 0.41428571428571104 1121 35-39 0.5534161490683118 1121 40-44 0.8229813664595866 1121 45-49 0.6233954451345625 1121 50-54 0.4873706004140743 1121 55-59 0.44637681159419174 1121 60-64 0.44720496894409223 1121 65-69 0.47670807453415165 1121 70-74 0.40890269151137204 1121 75-79 0.5108695652173836 1121 80-84 0.4166666666666572 1121 85-89 0.5655279503105461 1121 90-94 -0.4723602484472167 1121 95-99 0.6453416149068261 1121 100-104 0.6684265010351851 1121 105-109 0.7360248447204754 1121 110-114 1.1671842650103343 1121 115-119 0.9860248447204967 1121 120-124 1.2381987577639677 1121 125-129 1.6865424430641625 1121 130-134 0.786335403726703 1121 135-139 2.98944099378879 1121 140-144 3.367494824016539 1121 145-149 3.9486542443064394 1121 150 4.965838509316768 1124 1 0.3571428571428541 1124 2 0.24223602484472195 1124 3 0.48136645962733127 1124 4 0.29813664596273526 1124 5 0.30175983436853215 1124 6 0.4704968944099406 1124 7 0.366459627329192 1124 8 0.40062111801242395 1124 9 0.08281573498964434 1124 10-14 0.3698757763974996 1124 15-19 0.4339544513457483 1124 20-24 0.42826086956520726 1124 25-29 0.4900621118012225 1124 30-34 0.41428571428571104 1124 35-39 0.5534161490683118 1124 40-44 0.8229813664595866 1124 45-49 0.6233954451345625 1124 50-54 0.4873706004140743 1124 55-59 0.44637681159419174 1124 60-64 0.44720496894409223 1124 65-69 0.47670807453415165 1124 70-74 0.40890269151137204 1124 75-79 0.5108695652173836 1124 80-84 0.4166666666666572 1124 85-89 0.5655279503105461 1124 90-94 0.7276397515527862 1124 95-99 0.6453416149068261 1124 100-104 0.6684265010351851 1124 105-109 0.7360248447204754 1124 110-114 1.1671842650103343 1124 115-119 0.9860248447204967 1124 120-124 1.2381987577639677 1124 125-129 1.6865424430641625 1124 130-134 1.9863354037267058 1124 135-139 0.5894409937887914 1124 140-144 3.367494824016539 1124 145-149 3.9486542443064394 1124 150 4.965838509316768 1125 1 0.3571428571428541 1125 2 0.24223602484472195 1125 3 0.48136645962733127 1125 4 0.29813664596273526 1125 5 0.30175983436853215 1125 6 0.4704968944099406 1125 7 0.366459627329192 1125 8 0.40062111801242395 1125 9 0.08281573498964434 1125 10-14 0.3698757763974996 1125 15-19 0.4339544513457483 1125 20-24 -1.9717391304347913 1125 25-29 0.4900621118012225 1125 30-34 0.41428571428571104 1125 35-39 0.5534161490683118 1125 40-44 0.8229813664595866 1125 45-49 0.6233954451345625 1125 50-54 0.4873706004140743 1125 55-59 0.44637681159419174 1125 60-64 0.44720496894409223 1125 65-69 0.47670807453415165 1125 70-74 0.40890269151137204 1125 75-79 0.5108695652173836 1125 80-84 0.4166666666666572 1125 85-89 0.5655279503105461 1125 90-94 0.7276397515527862 1125 95-99 0.6453416149068261 1125 100-104 0.6684265010351851 1125 105-109 -1.6639751552795232 1125 110-114 1.1671842650103343 1125 115-119 0.9860248447204967 1125 120-124 1.2381987577639677 1125 125-129 1.6865424430641625 1125 130-134 -0.4136645962732928 1125 135-139 0.5894409937887914 1125 140-144 3.367494824016539 1125 145-149 1.5486542443064408 1125 150 4.965838509316768 1128 1 0.3571428571428541 1128 2 0.24223602484472195 1128 3 0.48136645962733127 1128 4 0.29813664596273526 1128 5 0.30175983436853215 1128 6 0.4704968944099406 1128 7 0.366459627329192 1128 8 0.40062111801242395 1128 9 0.08281573498964434 1128 10-14 0.3698757763974996 1128 15-19 0.4339544513457483 1128 20-24 0.42826086956520726 1128 25-29 0.4900621118012225 1128 30-34 0.41428571428571104 1128 35-39 0.5534161490683118 1128 40-44 0.8229813664595866 1128 45-49 0.6233954451345625 1128 50-54 0.4873706004140743 1128 55-59 0.44637681159419174 1128 60-64 0.44720496894409223 1128 65-69 0.47670807453415165 1128 70-74 0.40890269151137204 1128 75-79 -4.689130434782616 1128 80-84 0.4166666666666572 1128 85-89 0.5655279503105461 1128 90-94 0.7276397515527862 1128 95-99 0.6453416149068261 1128 100-104 0.6684265010351851 1128 105-109 0.7360248447204754 1128 110-114 1.1671842650103343 1128 115-119 0.9860248447204967 1128 120-124 -1.1618012422360309 1128 125-129 1.6865424430641625 1128 130-134 1.9863354037267058 1128 135-139 2.98944099378879 1128 140-144 3.367494824016539 1128 145-149 3.9486542443064394 1128 150 -21.034161490683232 1129 1 0.3571428571428541 1129 2 0.24223602484472195 1129 3 0.48136645962733127 1129 4 0.29813664596273526 1129 5 0.30175983436853215 1129 6 0.4704968944099406 1129 7 0.366459627329192 1129 8 0.40062111801242395 1129 9 0.08281573498964434 1129 10-14 0.3698757763974996 1129 15-19 0.4339544513457483 1129 20-24 0.42826086956520726 1129 25-29 0.4900621118012225 1129 30-34 0.41428571428571104 1129 35-39 0.5534161490683118 1129 40-44 0.8229813664595866 1129 45-49 0.6233954451345625 1129 50-54 0.4873706004140743 1129 55-59 0.44637681159419174 1129 60-64 0.44720496894409223 1129 65-69 0.47670807453415165 1129 70-74 0.40890269151137204 1129 75-79 0.5108695652173836 1129 80-84 0.4166666666666572 1129 85-89 0.5655279503105461 1129 90-94 -1.8723602484472153 1129 95-99 0.6453416149068261 1129 100-104 0.6684265010351851 1129 105-109 0.7360248447204754 1129 110-114 -0.0328157349896685 1129 115-119 -0.21397515527950617 1129 120-124 1.2381987577639677 1129 125-129 1.6865424430641625 1129 130-134 1.9863354037267058 1129 135-139 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2.98944099378879 2673 140-144 3.367494824016539 2673 145-149 3.9486542443064394 2673 150 4.965838509316768 2676 1 0.3571428571428541 2676 2 0.24223602484472195 2676 3 0.48136645962733127 2676 4 0.29813664596273526 2676 5 0.30175983436853215 2676 6 0.4704968944099406 2676 7 0.366459627329192 2676 8 0.40062111801242395 2676 9 0.08281573498964434 2676 10-14 0.3698757763974996 2676 15-19 0.4339544513457483 2676 20-24 0.42826086956520726 2676 25-29 0.4900621118012225 2676 30-34 0.41428571428571104 2676 35-39 0.5534161490683118 2676 40-44 0.8229813664595866 2676 45-49 0.6233954451345625 2676 50-54 0.4873706004140743 2676 55-59 0.44637681159419174 2676 60-64 0.44720496894409223 2676 65-69 -4.723291925465848 2676 70-74 -1.9910973084886265 2676 75-79 0.5108695652173836 2676 80-84 -1.9833333333333414 2676 85-89 0.5655279503105461 2676 90-94 0.7276397515527862 2676 95-99 0.6453416149068261 2676 100-104 0.6684265010351851 2676 105-109 -1.6639751552795232 2676 110-114 1.1671842650103343 2676 115-119 -1.413975155279502 2676 120-124 1.2381987577639677 2676 125-129 -0.7134575569358361 2676 130-134 1.9863354037267058 2676 135-139 2.98944099378879 2676 140-144 3.367494824016539 2676 145-149 -33.05134575569356 2676 150 -32.03416149068323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 0.0 25 1.0 26 1.0 27 5.0 28 6.0 29 3.0 30 13.0 31 24.0 32 31.0 33 57.0 34 92.0 35 340.0 36 3145.0 37 281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.7 13.0 7.425 45.875 2 19.084542271135568 12.156078039019508 33.06653326663332 35.692846423211606 3 17.525 17.125 32.824999999999996 32.525 4 22.225 17.1 29.775000000000002 30.9 5 27.325 27.700000000000003 26.075 18.9 6 20.474999999999998 33.775 27.125 18.625 7 18.825 26.1 37.55 17.525 8 16.925 37.425000000000004 25.35 20.3 9 14.85 32.725 33.975 18.45 10-14 16.134999999999998 29.409999999999997 28.360000000000003 26.095000000000002 15-19 23.115 30.165 22.32 24.4 20-24 20.625 32.385000000000005 21.759999999999998 25.230000000000004 25-29 16.04 33.975 22.98 27.005000000000003 30-34 17.474999999999998 28.835 27.07 26.619999999999997 35-39 20.085 25.615 29.9 24.4 40-44 21.805 25.264999999999997 26.455000000000002 26.474999999999998 45-49 17.04 25.174999999999997 28.325 29.459999999999997 50-54 15.28 24.89 37.435 22.395 55-59 21.8 21.529999999999998 36.655 20.015 60-64 20.119999999999997 22.595000000000002 29.805 27.48 65-69 19.38 25.064999999999998 27.02 28.535 70-74 23.865 24.82 27.52 23.794999999999998 75-79 23.95 23.525 23.044999999999998 29.48 80-84 21.192417346071124 22.79297754213975 30.955834542089733 25.058770569699394 85-89 21.43714971977582 26.441152922337867 24.96997598078463 27.151721377101683 90-94 20.17526289434151 23.42013019529294 30.215322984476717 26.189283925888834 95-99 27.35531797420067 24.243336846860412 28.750690157104852 19.65065502183406 100-104 23.71450113093742 31.18371450113094 19.592862528273436 25.508921839658207 105-109 18.229874641292856 35.50823138498716 21.960428938226855 24.301465035493127 110-114 19.254532563557174 25.032918059353793 32.19892636483338 23.513623012255646 115-119 19.36553271790687 22.35936145254246 25.649002907125006 32.626102922425666 120-124 16.666666666666664 30.739139386452543 24.17644636606959 28.4177475808112 125-129 16.150649350649353 27.26233766233766 28.436363636363637 28.15064935064935 130-134 20.866079434569336 25.644812490110237 26.504562476923887 26.984545598396544 135-139 25.85753395243974 22.652853078533468 24.84835471576574 26.641258253261046 140-144 20.35601179425576 27.42710494703505 28.497324451239486 23.719558807469696 145-149 19.76064569997217 28.34400222655163 25.143334261063178 26.752017812413026 150 21.29159616469261 26.79075014100395 30.823463056965593 21.094190637337846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 4.0 31 56.5 32 96.5 33 66.5 34 56.0 35 46.5 36 37.5 37 86.0 38 158.0 39 229.5 40 293.0 41 279.0 42 165.0 43 62.0 44 32.5 45 18.0 46 3.0 47 15.5 48 61.5 49 159.5 50 223.0 51 428.0 52 494.5 53 193.0 54 96.0 55 217.0 56 234.0 57 143.0 58 46.0 59 0.5 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 78-79 1.0 80-81 0.0 82-83 2.0 84-85 0.0 86-87 0.0 88-89 2.0 90-91 0.0 92-93 5.0 94-95 3.0 96-97 5.0 98-99 1.0 100-101 3.0 102-103 0.0 104-105 6.0 106-107 0.0 108-109 6.0 110-111 19.0 112-113 10.0 114-115 4.0 116-117 22.0 118-119 13.0 120-121 11.0 122-123 14.0 124-125 16.0 126-127 16.0 128-129 20.0 130-131 29.0 132-133 27.0 134-135 22.0 136-137 34.0 138-139 21.0 140-141 23.0 142-143 26.0 144-145 36.0 146-147 25.0 148-149 32.0 150-151 3546.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.075 #Duplication Level Percentage of deduplicated Percentage of total 1 33.592534992223946 5.4 2 19.284603421461895 6.2 3 12.130637636080872 5.8500000000000005 4 7.4650077760497675 4.8 5 3.421461897356143 2.75 6 3.7325038880248838 3.5999999999999996 7 2.021772939346812 2.275 8 2.177293934681182 2.8000000000000003 9 1.244167962674961 1.7999999999999998 >10 13.374805598755831 40.275 >50 1.088646967340591 10.925 >100 0.46656298600311047 13.325000000000001 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTC 223 5.575 No Hit CTCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCT 206 5.1499999999999995 No Hit GGGGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCATCAAT 104 2.6 No Hit CTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCT 74 1.8499999999999999 No Hit GTCAGCACCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCT 73 1.825 No Hit CCTCAGTGAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGGCTTTC 69 1.725 No Hit GGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGA 58 1.4500000000000002 No Hit CCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCGGCACCATGGACTGCA 56 1.4000000000000001 No Hit CCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCAGCACCATGGACTGCA 54 1.35 No Hit GTCCAAAACACTAGGGGAACATCATACACCAGCAAAAGCCATGAGATATA 53 1.325 No Hit CTCTATTTTACAATAAAGATGTAACACAAACACATTTTGTTTCTGAAGCA 50 1.25 No Hit CCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCTTTTTTTTC 45 1.125 No Hit ACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCTTTTTTTT 42 1.05 No Hit CTCAGTGAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGGCTTTCG 39 0.975 No Hit AGTGAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGGCTTTCGCAT 37 0.9249999999999999 No Hit GGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCATCAATTC 37 0.9249999999999999 No Hit GGGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCATCAATT 34 0.8500000000000001 No Hit CCTCAACTTTGATTCCTCTATTTTACAATAAAGATGTAACACAAACACAT 34 0.8500000000000001 No Hit GGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCGATGGCAAC 33 0.8250000000000001 No Hit GTTTCTGAAGCAGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAA 33 0.8250000000000001 No Hit GCTTCTTATTAGATGTACTTTGCATAAACAAACTTACAGGAAGTCCAAAA 33 0.8250000000000001 No Hit CTCCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCGGCACCATGGACTG 30 0.75 No Hit GCACTAGTCTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAATAAC 28 0.7000000000000001 No Hit CATCAATGTATTTTACTCTTTATTTATCTTGTTAGGATCAAATGAGGCAG 28 0.7000000000000001 No Hit GCATAAACAAACTTACAGGAAGTCCAAAACACTAGGGGAACATCATACAC 27 0.675 No Hit TCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTT 26 0.65 No Hit GTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCGATGGCA 25 0.625 No Hit GACCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGA 25 0.625 No Hit GGACCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGAACATCTTCTT 25 0.625 No Hit CAGCAAAAGCCATGAGATATACACAGTCATCAATTCATCATCTCAGCAAT 23 0.575 No Hit GCTCCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCGGCACCATGGACT 22 0.5499999999999999 No Hit CTAGGGGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCATC 22 0.5499999999999999 No Hit CTCAACTTTGATTCCTCTATTTTACAATAAAGATGTAACACAAACACATT 22 0.5499999999999999 No Hit AGGGGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCATCAA 21 0.525 No Hit CCTTAATTGATTATACACGCACTAGTCTCTCGTCCAGATAACAAATTCAA 21 0.525 No Hit CTTTGCATAAACAAACTTACAGGAAGTCCAAAACACTAGGGGAACATCAT 21 0.525 No Hit GCACCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCC 20 0.5 No Hit GCGTCAGCACCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGT 19 0.475 No Hit GCCATGAGATATACACAGTCATCAATTCATCATCTCAGCAATACAAAAGC 19 0.475 No Hit CCTCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCC 19 0.475 No Hit GCACCTCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCC 19 0.475 No Hit CCAGCAAAAGCCATGAGATATACACAGTCATCAATTCATCATCTCAGCAA 18 0.44999999999999996 No Hit CTTTATTTATCTTGTTAGGATCAAATGAGGCAGTATAATGTATAAACACA 18 0.44999999999999996 No Hit CCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGATT 18 0.44999999999999996 No Hit GCCTCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTC 18 0.44999999999999996 No Hit ACCTCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTC 17 0.42500000000000004 No Hit CACCTCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCT 17 0.42500000000000004 No Hit GCCTCAACTTTGATTCCTCTATTTTACAATAAAGATGTAACACAAACACA 17 0.42500000000000004 No Hit GACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGAC 16 0.4 No Hit AGGAAGTCCAAAACACTAGGGGAACATCATACACCAGCAAAAGCCATGAG 16 0.4 No Hit CACGCACTAGTCTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAAT 16 0.4 No Hit GGACATCTTTTTTTTACAGAAGGATCAGTGTTCCTTTCTAGGCAAGGCAG 16 0.4 No Hit ACGCACTAGTCTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAATA 16 0.4 No Hit TTTTTTTTTTTGTTTCTGAAGCAGTGGGAGCTATGTTTTTATTCATATTG 16 0.4 No Hit GCTCCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCAGCACCATGGACT 15 0.375 No Hit GTTAGGATCAAATGAGGCAGTATAATGTATAAACACATGTCCGAAGACCG 15 0.375 No Hit ATGAGATATACACAGTCATCAATTCATCATCTCAGCAATACAAAAGCAGA 15 0.375 No Hit ACACAGTCATCAATTCATCATCTCAGCAATACAAAAGCAGACTTGGAGAG 15 0.375 No Hit ACCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGAT 15 0.375 No Hit CTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGAACATCTT 15 0.375 No Hit GCAGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAAGTGTTGCGA 14 0.35000000000000003 No Hit CTATTTTACAATAAAGATGTAACACAAACACATTTTGTTTCTGAAGCAGT 14 0.35000000000000003 No Hit CCATGAGATATACACAGTCATCAATTCATCATCTCAGCAATACAAAAGCA 14 0.35000000000000003 No Hit CACAGTCATCAATTCATCATCTCAGCAATACAAAAGCAGACTTGGAGAGT 14 0.35000000000000003 No Hit CCTGGCTTGACCATCAACCAAAGAGAAGGAAAACAAATTATCTGTGAGTT 14 0.35000000000000003 No Hit CCTCTATTTTACAATAAAGATGTAACACAAACACATTTTGTTTCTGAAGC 14 0.35000000000000003 No Hit CTGGATCTTCTCCTCAGTGAATGGAAATGCGTCGGCACCATGGACTGCAA 13 0.325 No Hit TAGGGGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCATCA 13 0.325 No Hit GCACCCTCAACCCTCATATCAAGGGTTCAGGCCTCAACTTTGATTCCTCT 13 0.325 No Hit GCTACAACAGGAACCTTCAAGGTATTTAGCTTTGCTCTTCCTCAGCGCCC 13 0.325 No Hit ACCAGCAAAAGCCATGAGATATACACAGTCATCAATTCATCATCTCAGCA 13 0.325 No Hit ACACGCACTAGTCTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAA 12 0.3 No Hit CACTAGTCTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAATAACA 12 0.3 No Hit GGCCTCAACTTTGATTCCTCTATTTTACAATAAAGATGTAACACAAACAC 12 0.3 No Hit AGCTCCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCGGCACCATGGAC 12 0.3 No Hit CCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACT 12 0.3 No Hit GGCGTCAGCACCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCG 12 0.3 No Hit CTTAATTGATTATACACGCACTAGTCTCTCGTCCAGATAACAAATTCAAA 12 0.3 No Hit CACTAGGGGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCA 11 0.27499999999999997 No Hit CTTCTCCTCAGTGAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGG 11 0.27499999999999997 No Hit CTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAATAACAGTAAAAT 11 0.27499999999999997 No Hit GGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCGATGGCAACA 11 0.27499999999999997 No Hit TGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCTTT 11 0.27499999999999997 No Hit CTCTATCAGAAGGCACATCTGAACTCAGGGCAGGGCCTCGGCTCATAATA 11 0.27499999999999997 No Hit CAGTGAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGGCTTTCGCA 11 0.27499999999999997 No Hit CACCAGCAAAAGCCATGAGATATACACAGTCATCAATTCATCATCTCAGC 11 0.27499999999999997 No Hit CCATCAATGTATTTTACTCTTTATTTATCTTGTTAGGATCAAATGAGGCA 11 0.27499999999999997 No Hit TTCTGAAGCAGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAAGT 11 0.27499999999999997 No Hit ACAGGAAGTCCAAAACACTAGGGGAACATCATACACCAGCAAAAGCCATG 10 0.25 No Hit GTATAATGTATAAACACATGTCCGAAGACCGCAAACTTCCAACGAAGAAA 10 0.25 No Hit GTGAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGGCTTTCGCATC 10 0.25 No Hit CCCCTGTTGACTCAGCATCATCTCCATCGGAAGGCTCATCTGTACTTGGA 10 0.25 No Hit CTTCAAGGTATTTAGCTTTGCTCTTCCTCAGCGCCCGCCGCCTCCCTCTC 10 0.25 No Hit AGCACCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCC 10 0.25 No Hit CTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCGATGGCAACAAGA 10 0.25 No Hit CTTTGGACATCTTTTTTTTACAGAAGGATCAGTGTTCCTTTCTAGGCAAG 10 0.25 No Hit CTCCTCAGTGAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGGCTT 9 0.22499999999999998 No Hit GCAGTATAATGTATAAACACATGTCCGAAGACCGCAAACTTCCAACGAAG 9 0.22499999999999998 No Hit GTCCAGATAACAAATTCAAACACAGGAGAGAAATAACAGTAAAATTAAGC 9 0.22499999999999998 No Hit GATCAAATGAGGCAGTATAATGTATAAACACATGTCCGAAGACCGCAAAC 9 0.22499999999999998 No Hit GAATGGAAATGCGTCAGCACCATGGACTGCAAGTGGGGCTTTCGCATCTG 9 0.22499999999999998 No Hit CAGCACCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTC 9 0.22499999999999998 No Hit GCCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCTTTTTTTT 9 0.22499999999999998 No Hit ACCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGAACATCTTCTTTA 9 0.22499999999999998 No Hit CCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGAACATCTTCTTTAA 8 0.2 No Hit GACCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGAACATCTTCTTT 8 0.2 No Hit CAGGAAGTCCAAAACACTAGGGGAACATCATACACCAGCAAAAGCCATGA 8 0.2 No Hit GTGTTGACCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCC 8 0.2 No Hit GTTGACCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCAC 8 0.2 No Hit CATGAGATATACACAGTCATCAATTCATCATCTCAGCAATACAAAAGCAG 8 0.2 No Hit CTTTTTTTTACAGAAGGATCAGTGTTCCTTTCTAGGCAAGGCAGAGATTT 8 0.2 No Hit ATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGG 8 0.2 No Hit GGCAGTATAATGTATAAACACATGTCCGAAGACCGCAAACTTCCAACGAA 8 0.2 No Hit GCAACATCTCTATCAGAAGGCACATCTGAACTCAGGGCAGGGCCTCGGCT 8 0.2 No Hit TCTGTGTTGACCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGAT 8 0.2 No Hit AGGCAGTATAATGTATAAACACATGTCCGAAGACCGCAAACTTCCAACGA 8 0.2 No Hit GCCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGAT 8 0.2 No Hit GAGATATACACAGTCATCAATTCATCATCTCAGCAATACAAAAGCAGACT 8 0.2 No Hit CTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCG 7 0.17500000000000002 No Hit CTGGATCTTCTCCTCAGTGAATGGAAATGCGTCAGCACCATGGACTGCAA 7 0.17500000000000002 No Hit GTTCCAACAAGTCTGCTTGCTTTTGTTTGAGTGGCATCTGATGGGAATAT 7 0.17500000000000002 No Hit CAGCTCCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCAGCACCATGGA 7 0.17500000000000002 No Hit CTAGTCTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAATAACAGT 7 0.17500000000000002 No Hit GGGACCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGAACATCTTCT 7 0.17500000000000002 No Hit ACCCTGTTGACTCAGCATCATCTCCATCGGAAGGCTCATCTGTACTTGGA 7 0.17500000000000002 No Hit TGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCGATGGCAA 7 0.17500000000000002 No Hit CACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGAACATC 7 0.17500000000000002 No Hit ACACAAACACATTTTGTTTCTGAAGCAGTGGGAGCTATGTTTTTATTCAT 7 0.17500000000000002 No Hit GGACCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGG 7 0.17500000000000002 No Hit AGCTCCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCAGCACCATGGAC 7 0.17500000000000002 No Hit CCCTGTTGACTCAGCATCATCTCCATCGGAAGGCTCATCTGTACTTGGAG 7 0.17500000000000002 No Hit GATTCCTCTATTTTACAATAAAGATGTAACACAAACACATTTTGTTTCTG 6 0.15 No Hit GTTTGACCATCAACCAAAGAGAAGGAAAACAAATTATCTGTGAGTTTGGA 6 0.15 No Hit CACATTTTGTTTCTGAAGCAGTGGGAGCTATGTTTTTATTCATATTGTTT 6 0.15 No Hit CTCGGCTCATAATAAGAACCCCTGTTGACTCAGCATCATCTCCATCGGAA 6 0.15 No Hit ATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCTTTTT 6 0.15 No Hit CTTACAGGAAGTCCAAAACACTAGGGGAACATCATACACCAGCAAAAGCC 6 0.15 No Hit CCTCAGTGAATGGAAATGCGTCGGCACCATGGACTGCAAGTGGGGCTTTC 6 0.15 No Hit CCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTT 6 0.15 No Hit CTCTTTATTTATCTTGTTAGGATCAAATGAGGCAGTATAATGTATAAACA 6 0.15 No Hit GGCACATCTGAACTCAGGGCAGGGCCTCGGCTCATAATAAGAACCCCTGT 6 0.15 No Hit CAGGTGCAAAAATGCTTCTTATTAGATGTACTTTGCATAAACAAACTTAC 6 0.15 No Hit CAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCGATG 6 0.15 No Hit CTGAAGCAGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAAGTGT 6 0.15 No Hit CTTCTTATTAGATGTACTTTGCATAAACAAACTTACAGGAAGTCCAAAAC 6 0.15 No Hit TTTTTTTTTTGTTTCTGAAGCAGTGGGAGCTATGTTTTTATTCATATTGT 6 0.15 No Hit CTCCTTTGGACATCTTTTTTTTACAGAAGGATCAGTGTTCCTTTCTAGGC 6 0.15 No Hit CCTCGGCTCATAATAAGAACCCCTGTTGACTCAGCATCATCTCCATCGGA 6 0.15 No Hit GCACAGTCATCAATTCATCATCTCAGCAATACAAAAGCAGACTTGGAGAG 6 0.15 No Hit AGAGAAATAACAGTAAAATTAAGCTACAACAGGAACCTTCAAGGTATTTA 6 0.15 No Hit AGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAAGTGTTGCGATT 6 0.15 No Hit CCCGTCTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGAT 6 0.15 No Hit GGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGACCGATGGCAACAA 6 0.15 No Hit TTTCTGAAGCAGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAAG 6 0.15 No Hit CAGGAGAGAAATAACAGTAAAATTAAGCTACAACAGGAACCTTCAAGGTA 6 0.15 No Hit GCACCAGCAAAAGCCATGAGATATACACAGTCATCAATTCATCATCTCAG 5 0.125 No Hit ACCTGTTGACTCAGCATCATCTCCATCGGAAGGCTCATCTGTACTTGGAG 5 0.125 No Hit CTTCCCACTGGGACCGATGGCAACAAGAGAAGGGATCCCACGGATTTTGA 5 0.125 No Hit ACTAGGGGAACATCATACACCAGCAAAAGCCATGAGATATACACAGTCAT 5 0.125 No Hit CTGTTGACTCAGCATCATCTCCATCGGAAGGCTCATCTGTACTTGGAGCA 5 0.125 No Hit ACATCATACACCAGCAAAAGCCATGAGATATACACAGTCATCAATTCATC 5 0.125 No Hit GTTCAGGCCTCAACTTTGATTCCTCTATTTTACAATAAAGATGTAACACA 5 0.125 No Hit CACATCTGAACTCAGGGCAGGGCCTCGGCTCATAATAAGAACCCCTGTTG 5 0.125 No Hit GGCCTTAACTTTGATTCCTCTATTTTACAATAAAGATGTAACACAAACAC 5 0.125 No Hit AGAAATAACAGTAAAATTAAGCTACAACAGGAACCTTCAAGGTATTTAGC 5 0.125 No Hit GTCGGCACCATGGACTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCT 5 0.125 No Hit CGCACTAGTCTCTCGTCCAGATAACAAATTCAAACACAGGAGAGAAATAA 5 0.125 No Hit GTCCTGGATCTTCTCCTCAGTGAATGGAAATGCGTCAGCACCATGGACTG 5 0.125 No Hit TTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCTT 5 0.125 No Hit GGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAAGTGTTGCGATT 5 0.125 No Hit CAGATAACAAATTCAAACACAGGAGAGAAATAACAGTAAAATTAAGCTAC 5 0.125 No Hit GAGGCAGTATAATGTATAAACACATGTCCGAAGACCGCAAACTTCCAACG 5 0.125 No Hit GGCAGGGCCTCGGCTCATAATAAGAACCCCTGTTGACTCAGCATCATCTC 5 0.125 No Hit CCTGTTGACTCAGCATCATCTCCATCGGAAGGCTCATCTGTACTTGGAGC 5 0.125 No Hit TTTTTTTTTTCTGAAGCAGTGGGAGCTATGTTTTTATTCATATTGTTTCT 5 0.125 No Hit GGGCTGCAAGTGGGGCTTTCGCATCTGTGTTGACCGTCTTCCCACTGGGA 5 0.125 No Hit CAGTGGGAGCTATGTTTTTATTCATATTGTTTCTGAACAAGTGTTGCGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATCTT 25 0.0010530235 82.95 4 GATCTTC 25 0.0010530235 82.95 5 CTGGATC 25 0.0010530235 82.95 2 TGGATCT 25 0.0010530235 82.95 3 CCTGGAT 25 0.0010530235 82.95 1 TCTTCTC 30 0.0021715884 69.12501 7 CCTTGAT 55 3.602127E-4 50.272724 1 GATAACC 65 8.2112424E-4 42.538464 5 AACCTTG 65 8.2112424E-4 42.538464 8 TGATAAC 65 8.2112424E-4 42.538464 4 ATAACCT 65 8.2112424E-4 42.538464 6 TAACCTT 65 8.2112424E-4 42.538464 7 TTGATAA 65 8.2112424E-4 42.538464 3 CTTGATA 65 8.2112424E-4 42.538464 2 ACCTTGC 70 0.0010535351 39.5 9 TAAGTAC 20 0.002422463 34.779877 140-144 TGGCATA 65 1.4733814E-10 29.429125 140-144 GGCACCA 20 0.0074910643 27.650002 35-39 GCGTCGG 20 0.0074910643 27.650002 30-34 TCTCCTC 25 6.579056E-4 27.65 10-14 >>END_MODULE ERR5262805 read2 length is 79-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262805_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 79-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.155 37.0 37.0 37.0 37.0 37.0 2 35.9305 37.0 37.0 37.0 37.0 37.0 3 36.052 37.0 37.0 37.0 37.0 37.0 4 36.1205 37.0 37.0 37.0 37.0 37.0 5 36.1885 37.0 37.0 37.0 37.0 37.0 6 36.151 37.0 37.0 37.0 37.0 37.0 7 36.141 37.0 37.0 37.0 37.0 37.0 8 36.2625 37.0 37.0 37.0 37.0 37.0 9 36.2105 37.0 37.0 37.0 37.0 37.0 10-14 36.2344 37.0 37.0 37.0 37.0 37.0 15-19 36.2473 37.0 37.0 37.0 37.0 37.0 20-24 36.2184 37.0 37.0 37.0 37.0 37.0 25-29 36.1798 37.0 37.0 37.0 37.0 37.0 30-34 36.212199999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.1119 37.0 37.0 37.0 37.0 37.0 40-44 36.1589 37.0 37.0 37.0 37.0 37.0 45-49 36.14319999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.15089999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.1031 37.0 37.0 37.0 37.0 37.0 60-64 36.073 37.0 37.0 37.0 37.0 37.0 65-69 36.06100000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.083400000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.9961 37.0 37.0 37.0 37.0 37.0 80-84 35.90331554617451 37.0 37.0 37.0 37.0 37.0 85-89 36.00009812163928 37.0 37.0 37.0 37.0 37.0 90-94 35.94131147643829 37.0 37.0 37.0 37.0 37.0 95-99 35.87497059669845 37.0 37.0 37.0 37.0 37.0 100-104 35.8154687296168 37.0 37.0 37.0 37.0 37.0 105-109 35.810831757340964 37.0 37.0 37.0 37.0 37.0 110-114 35.7103989124992 37.0 37.0 37.0 37.0 37.0 115-119 35.74170533547948 37.0 37.0 37.0 37.0 37.0 120-124 35.75036259840849 37.0 37.0 37.0 37.0 37.0 125-129 35.687146698684344 37.0 37.0 37.0 37.0 37.0 130-134 35.672980000565865 37.0 37.0 37.0 37.0 37.0 135-139 35.640704787174855 37.0 37.0 37.0 37.0 37.0 140-144 35.64170095804119 37.0 37.0 37.0 37.0 37.0 145-149 35.6026627430951 37.0 37.0 37.0 37.0 37.0 150 35.65347654041832 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1104 1 0.8126293995859228 1104 2 0.9844720496894439 1104 3 0.7391304347826093 1104 4 0.950310559006212 1104 5 0.8198757763975166 1104 6 0.6045548654244328 1104 7 0.6816770186335432 1104 8 0.5093167701863379 1104 9 0.8369565217391326 1104 10-14 0.6263975155279411 1104 15-19 0.5553830227743219 1104 20-24 0.725362318840574 1104 25-29 0.6515527950310513 1104 30-34 0.6965838509316669 1104 35-39 0.7316770186335333 1104 40-44 0.6086956521739211 1104 45-49 -1.6193581780538366 1104 50-54 0.63788819875775 1104 55-59 0.7638716356107551 1104 60-64 0.8305383022774109 1104 65-69 0.8188405797101339 1104 70-74 0.6936853002070364 1104 75-79 -1.4919254658385057 1104 80-84 -1.4086956521739182 1104 85-89 1.107556935817776 1104 90-94 0.9786749482401405 1104 95-99 0.9714285714285609 1104 100-104 1.1663561076604338 1104 105-109 0.9813664596273242 1104 110-114 1.3407867494824046 1104 115-119 1.5669772256728578 1104 120-124 1.6844720496894183 1104 125-129 1.9418219461697532 1104 130-134 2.479606625258775 1104 135-139 3.2587991718426323 1104 140-144 -1.5163561076604744 1104 145-149 -0.5162525879917439 1104 150 5.142857142857142 1105 1 0.8126293995859228 1105 2 0.9844720496894439 1105 3 0.7391304347826093 1105 4 0.950310559006212 1105 5 0.8198757763975166 1105 6 0.6045548654244328 1105 7 0.6816770186335432 1105 8 0.5093167701863379 1105 9 0.8369565217391326 1105 10-14 0.6263975155279411 1105 15-19 0.5553830227743219 1105 20-24 0.725362318840574 1105 25-29 0.6515527950310513 1105 30-34 0.6965838509316669 1105 35-39 0.7316770186335333 1105 40-44 0.6086956521739211 1105 45-49 0.7806418219461619 1105 50-54 0.63788819875775 1105 55-59 -1.6361283643892435 1105 60-64 0.8305383022774109 1105 65-69 0.8188405797101339 1105 70-74 0.6936853002070364 1105 75-79 0.9080745341614929 1105 80-84 0.9913043478260803 1105 85-89 1.107556935817776 1105 90-94 0.9786749482401405 1105 95-99 0.9714285714285609 1105 100-104 1.1663561076604338 1105 105-109 0.9813664596273242 1105 110-114 1.3407867494824046 1105 115-119 1.5669772256728578 1105 120-124 1.6844720496894183 1105 125-129 1.9418219461697532 1105 130-134 2.479606625258775 1105 135-139 3.2587991718426323 1105 140-144 3.683643892339525 1105 145-149 4.283747412008253 1105 150 5.142857142857142 1107 1 0.8126293995859228 1107 2 0.9844720496894439 1107 3 0.7391304347826093 1107 4 0.950310559006212 1107 5 0.8198757763975166 1107 6 0.6045548654244328 1107 7 0.6816770186335432 1107 8 0.5093167701863379 1107 9 0.8369565217391326 1107 10-14 0.6263975155279411 1107 15-19 0.5553830227743219 1107 20-24 0.725362318840574 1107 25-29 0.6515527950310513 1107 30-34 0.6965838509316669 1107 35-39 0.7316770186335333 1107 40-44 0.6086956521739211 1107 45-49 0.7806418219461619 1107 50-54 -0.5621118012422528 1107 55-59 0.7638716356107551 1107 60-64 0.8305383022774109 1107 65-69 -0.38115942028986893 1107 70-74 -0.5063146997929664 1107 75-79 0.9080745341614929 1107 80-84 0.9913043478260803 1107 85-89 1.107556935817776 1107 90-94 0.9786749482401405 1107 95-99 0.9714285714285609 1107 100-104 1.1663561076604338 1107 105-109 -1.6186335403726773 1107 110-114 1.3407867494824046 1107 115-119 0.36697722567285496 1107 120-124 1.6844720496894183 1107 125-129 1.9418219461697532 1107 130-134 2.479606625258775 1107 135-139 -0.5412008281573648 1107 140-144 3.683643892339525 1107 145-149 -2.1162525879917453 1107 150 5.142857142857142 1109 1 -11.187370600414077 1109 2 0.9844720496894439 1109 3 0.7391304347826093 1109 4 -11.049689440993788 1109 5 0.8198757763975166 1109 6 0.6045548654244328 1109 7 0.6816770186335432 1109 8 0.5093167701863379 1109 9 0.8369565217391326 1109 10-14 0.6263975155279411 1109 15-19 0.5553830227743219 1109 20-24 0.725362318840574 1109 25-29 0.6515527950310513 1109 30-34 0.6965838509316669 1109 35-39 0.7316770186335333 1109 40-44 0.6086956521739211 1109 45-49 0.7806418219461619 1109 50-54 0.63788819875775 1109 55-59 0.7638716356107551 1109 60-64 0.8305383022774109 1109 65-69 0.8188405797101339 1109 70-74 0.6936853002070364 1109 75-79 0.9080745341614929 1109 80-84 0.9913043478260803 1109 85-89 1.107556935817776 1109 90-94 0.9786749482401405 1109 95-99 0.9714285714285609 1109 100-104 1.1663561076604338 1109 105-109 0.9813664596273242 1109 110-114 1.3407867494824046 1109 115-119 1.5669772256728578 1109 120-124 1.6844720496894183 1109 125-129 1.9418219461697532 1109 130-134 2.479606625258775 1109 135-139 3.2587991718426323 1109 140-144 3.683643892339525 1109 145-149 4.283747412008253 1109 150 5.142857142857142 1111 1 0.8126293995859228 1111 2 0.9844720496894439 1111 3 0.7391304347826093 1111 4 0.950310559006212 1111 5 0.8198757763975166 1111 6 0.6045548654244328 1111 7 -11.318322981366457 1111 8 0.5093167701863379 1111 9 -11.163043478260867 1111 10-14 -1.7736024844720575 1111 15-19 0.5553830227743219 1111 20-24 0.725362318840574 1111 25-29 0.6515527950310513 1111 30-34 0.6965838509316669 1111 35-39 -4.468322981366466 1111 40-44 0.6086956521739211 1111 45-49 0.7806418219461619 1111 50-54 0.63788819875775 1111 55-59 0.7638716356107551 1111 60-64 0.8305383022774109 1111 65-69 0.8188405797101339 1111 70-74 0.6936853002070364 1111 75-79 0.9080745341614929 1111 80-84 0.9913043478260803 1111 85-89 -4.092443064182223 1111 90-94 0.9786749482401405 1111 95-99 0.9714285714285609 1111 100-104 -6.433643892339568 1111 105-109 0.9813664596273242 1111 110-114 1.3407867494824046 1111 115-119 1.5669772256728578 1111 120-124 1.6844720496894183 1111 125-129 1.9418219461697532 1111 130-134 0.07960662525877638 1111 135-139 3.2587991718426323 1111 140-144 3.683643892339525 1111 145-149 4.283747412008253 1111 150 -31.857142857142858 1112 1 0.8126293995859228 1112 2 0.9844720496894439 1112 3 0.7391304347826093 1112 4 0.950310559006212 1112 5 0.8198757763975166 1112 6 0.6045548654244328 1112 7 0.6816770186335432 1112 8 0.5093167701863379 1112 9 0.8369565217391326 1112 10-14 0.6263975155279411 1112 15-19 0.5553830227743219 1112 20-24 0.725362318840574 1112 25-29 0.6515527950310513 1112 30-34 0.6965838509316669 1112 35-39 0.7316770186335333 1112 40-44 0.6086956521739211 1112 45-49 0.7806418219461619 1112 50-54 0.63788819875775 1112 55-59 0.7638716356107551 1112 60-64 0.8305383022774109 1112 65-69 0.8188405797101339 1112 70-74 0.6936853002070364 1112 75-79 0.9080745341614929 1112 80-84 0.9913043478260803 1112 85-89 1.107556935817776 1112 90-94 0.9786749482401405 1112 95-99 0.9714285714285609 1112 100-104 1.1663561076604338 1112 105-109 0.9813664596273242 1112 110-114 1.3407867494824046 1112 115-119 1.5669772256728578 1112 120-124 1.6844720496894183 1112 125-129 1.9418219461697532 1112 130-134 2.479606625258775 1112 135-139 3.2587991718426323 1112 140-144 3.683643892339525 1112 145-149 -32.71625258799175 1112 150 -31.857142857142858 1114 1 0.8126293995859228 1114 2 -5.015527950310556 1114 3 0.7391304347826093 1114 4 0.950310559006212 1114 5 0.8198757763975166 1114 6 0.6045548654244328 1114 7 0.6816770186335432 1114 8 0.5093167701863379 1114 9 0.8369565217391326 1114 10-14 0.6263975155279411 1114 15-19 0.5553830227743219 1114 20-24 0.725362318840574 1114 25-29 0.6515527950310513 1114 30-34 0.6965838509316669 1114 35-39 0.7316770186335333 1114 40-44 0.6086956521739211 1114 45-49 0.7806418219461619 1114 50-54 0.63788819875775 1114 55-59 0.7638716356107551 1114 60-64 0.8305383022774109 1114 65-69 -0.38115942028986893 1114 70-74 0.6936853002070364 1114 75-79 0.9080745341614929 1114 80-84 0.9913043478260803 1114 85-89 1.107556935817776 1114 90-94 0.9786749482401405 1114 95-99 0.9714285714285609 1114 100-104 1.1663561076604338 1114 105-109 -0.21863354037267868 1114 110-114 1.3407867494824046 1114 115-119 1.5669772256728578 1114 120-124 1.6844720496894183 1114 125-129 1.9418219461697532 1114 130-134 2.479606625258775 1114 135-139 2.0587991718426295 1114 140-144 3.683643892339525 1114 145-149 4.283747412008253 1114 150 5.142857142857142 1121 1 0.8126293995859228 1121 2 0.9844720496894439 1121 3 0.7391304347826093 1121 4 0.950310559006212 1121 5 0.8198757763975166 1121 6 0.6045548654244328 1121 7 0.6816770186335432 1121 8 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0.9913043478260803 2655 85-89 0.5075569358177745 2655 90-94 -0.9213250517598581 2655 95-99 -0.22857142857144197 2655 100-104 -0.6336438923395633 2655 105-109 -0.318633540372673 2655 110-114 -1.059213250517594 2655 115-119 -1.5330227743271436 2655 120-124 1.084472049689417 2655 125-129 -2.4581780538302453 2655 130-134 1.2796066252587721 2655 135-139 0.058799171842629505 2655 140-144 1.2836438923395264 2655 145-149 0.5837474120082504 2655 150 2.1428571428571423 2659 1 0.8126293995859228 2659 2 0.9844720496894439 2659 3 0.7391304347826093 2659 4 0.950310559006212 2659 5 0.8198757763975166 2659 6 0.6045548654244328 2659 7 -11.318322981366457 2659 8 0.5093167701863379 2659 9 -25.163043478260867 2659 10-14 -1.7736024844720575 2659 15-19 0.5553830227743219 2659 20-24 0.725362318840574 2659 25-29 0.6515527950310513 2659 30-34 -6.903416149068335 2659 35-39 0.7316770186335333 2659 40-44 0.6086956521739211 2659 45-49 -1.6193581780538366 2659 50-54 -1.7621118012422485 2659 55-59 -1.6361283643892435 2659 60-64 -6.7694616977225905 2659 65-69 -6.7811594202898675 2659 70-74 0.6936853002070364 2659 75-79 -1.4919254658385057 2659 80-84 -11.80869565217392 2659 85-89 1.107556935817776 2659 90-94 0.9786749482401405 2659 95-99 0.9714285714285609 2659 100-104 1.1663561076604338 2659 105-109 -6.618633540372677 2659 110-114 1.3407867494824046 2659 115-119 -0.8330227743271408 2659 120-124 -0.7155279503105803 2659 125-129 -2.858178053830244 2659 130-134 2.479606625258775 2659 135-139 3.2587991718426323 2659 140-144 1.2836438923395264 2659 145-149 -3.316252587991748 2659 150 -6.857142857142858 2660 1 0.8126293995859228 2660 2 0.9844720496894439 2660 3 0.7391304347826093 2660 4 0.950310559006212 2660 5 0.8198757763975166 2660 6 0.6045548654244328 2660 7 0.6816770186335432 2660 8 0.5093167701863379 2660 9 0.8369565217391326 2660 10-14 0.6263975155279411 2660 15-19 0.5553830227743219 2660 20-24 0.725362318840574 2660 25-29 0.6515527950310513 2660 30-34 0.6965838509316669 2660 35-39 0.7316770186335333 2660 40-44 -1.7913043478260775 2660 45-49 0.7806418219461619 2660 50-54 0.63788819875775 2660 55-59 0.7638716356107551 2660 60-64 0.8305383022774109 2660 65-69 -4.381159420289865 2660 70-74 0.6936853002070364 2660 75-79 -4.291925465838506 2660 80-84 0.9913043478260803 2660 85-89 -1.2924430641822227 2660 90-94 0.9786749482401405 2660 95-99 -1.4285714285714377 2660 100-104 -3.6336438923395633 2660 105-109 -4.218633540372675 2660 110-114 -1.059213250517594 2660 115-119 1.5669772256728578 2660 120-124 -0.7155279503105803 2660 125-129 1.9418219461697532 2660 130-134 0.07960662525877638 2660 135-139 3.2587991718426323 2660 140-144 3.683643892339525 2660 145-149 4.283747412008253 2660 150 5.142857142857142 2661 1 0.8126293995859228 2661 2 0.9844720496894439 2661 3 0.7391304347826093 2661 4 0.950310559006212 2661 5 0.8198757763975166 2661 6 0.6045548654244328 2661 7 0.6816770186335432 2661 8 0.5093167701863379 2661 9 0.8369565217391326 2661 10-14 0.6263975155279411 2661 15-19 -1.8446169772256766 2661 20-24 0.725362318840574 2661 25-29 0.6515527950310513 2661 30-34 0.6965838509316669 2661 35-39 0.7316770186335333 2661 40-44 0.6086956521739211 2661 45-49 0.7806418219461619 2661 50-54 0.63788819875775 2661 55-59 0.7638716356107551 2661 60-64 0.8305383022774109 2661 65-69 0.8188405797101339 2661 70-74 0.6936853002070364 2661 75-79 0.9080745341614929 2661 80-84 0.9913043478260803 2661 85-89 1.107556935817776 2661 90-94 0.9786749482401405 2661 95-99 0.9714285714285609 2661 100-104 1.1663561076604338 2661 105-109 0.9813664596273242 2661 110-114 1.3407867494824046 2661 115-119 1.5669772256728578 2661 120-124 1.6844720496894183 2661 125-129 1.9418219461697532 2661 130-134 2.479606625258775 2661 135-139 -33.74120082815737 2661 140-144 -33.316356107660475 2661 145-149 -32.71625258799175 2661 150 -31.857142857142858 2665 1 0.8126293995859228 2665 2 0.9844720496894439 2665 3 0.7391304347826093 2665 4 0.950310559006212 2665 5 0.8198757763975166 2665 6 0.6045548654244328 2665 7 0.6816770186335432 2665 8 0.5093167701863379 2665 9 0.8369565217391326 2665 10-14 0.6263975155279411 2665 15-19 0.5553830227743219 2665 20-24 0.725362318840574 2665 25-29 0.6515527950310513 2665 30-34 0.6965838509316669 2665 35-39 0.7316770186335333 2665 40-44 0.6086956521739211 2665 45-49 0.7806418219461619 2665 50-54 0.63788819875775 2665 55-59 0.7638716356107551 2665 60-64 -4.369461697722588 2665 65-69 0.8188405797101339 2665 70-74 0.6936853002070364 2665 75-79 0.9080745341614929 2665 80-84 -4.208695652173919 2665 85-89 1.107556935817776 2665 90-94 0.9786749482401405 2665 95-99 0.9714285714285609 2665 100-104 1.1663561076604338 2665 105-109 0.9813664596273242 2665 110-114 -1.059213250517594 2665 115-119 1.5669772256728578 2665 120-124 1.6844720496894183 2665 125-129 1.9418219461697532 2665 130-134 2.479606625258775 2665 135-139 3.2587991718426323 2665 140-144 3.683643892339525 2665 145-149 4.283747412008253 2665 150 5.142857142857142 2668 1 0.8126293995859228 2668 2 0.9844720496894439 2668 3 0.7391304347826093 2668 4 -11.049689440993788 2668 5 -25.180124223602483 2668 6 0.6045548654244328 2668 7 0.6816770186335432 2668 8 0.5093167701863379 2668 9 0.8369565217391326 2668 10-14 0.6263975155279411 2668 15-19 0.5553830227743219 2668 20-24 -1.6746376811594246 2668 25-29 0.6515527950310513 2668 30-34 -1.7034161490683317 2668 35-39 -6.868322981366468 2668 40-44 0.6086956521739211 2668 45-49 0.7806418219461619 2668 50-54 -4.562111801242249 2668 55-59 -9.636128364389243 2668 60-64 -4.369461697722588 2668 65-69 -4.381159420289865 2668 70-74 -4.506314699792963 2668 75-79 -1.4919254658385057 2668 80-84 0.9913043478260803 2668 85-89 -6.4924430641822255 2668 90-94 -3.8213250517598567 2668 95-99 -4.228571428571438 2668 100-104 -14.433643892339568 2668 105-109 -1.4186335403726744 2668 110-114 -3.8592132505175947 2668 115-119 -8.433022774327142 2668 120-124 -3.515527950310581 2668 125-129 1.9418219461697532 2668 130-134 -7.920393374741224 2668 135-139 -1.941200828157367 2668 140-144 -1.5163561076604744 2668 145-149 4.283747412008253 2668 150 5.142857142857142 2672 1 0.8126293995859228 2672 2 0.9844720496894439 2672 3 0.7391304347826093 2672 4 0.950310559006212 2672 5 -5.180124223602483 2672 6 0.6045548654244328 2672 7 0.6816770186335432 2672 8 0.5093167701863379 2672 9 0.8369565217391326 2672 10-14 0.6263975155279411 2672 15-19 0.5553830227743219 2672 20-24 0.725362318840574 2672 25-29 -0.5484472049689515 2672 30-34 0.6965838509316669 2672 35-39 -1.6683229813664653 2672 40-44 0.6086956521739211 2672 45-49 0.7806418219461619 2672 50-54 0.63788819875775 2672 55-59 -1.6361283643892435 2672 60-64 -2.9694616977225863 2672 65-69 -1.5811594202898647 2672 70-74 0.6936853002070364 2672 75-79 -6.491925465838506 2672 80-84 -2.808695652173917 2672 85-89 1.107556935817776 2672 90-94 -0.2213250517598624 2672 95-99 0.9714285714285609 2672 100-104 -6.433643892339568 2672 105-109 -0.21863354037267868 2672 110-114 -3.6592132505175954 2672 115-119 1.5669772256728578 2672 120-124 -0.7155279503105803 2672 125-129 1.9418219461697532 2672 130-134 0.07960662525877638 2672 135-139 0.8587991718426338 2672 140-144 3.683643892339525 2672 145-149 4.283747412008253 2672 150 5.142857142857142 2673 1 0.8126293995859228 2673 2 0.9844720496894439 2673 3 0.7391304347826093 2673 4 0.950310559006212 2673 5 0.8198757763975166 2673 6 0.6045548654244328 2673 7 0.6816770186335432 2673 8 0.5093167701863379 2673 9 0.8369565217391326 2673 10-14 0.6263975155279411 2673 15-19 0.5553830227743219 2673 20-24 0.725362318840574 2673 25-29 0.6515527950310513 2673 30-34 0.6965838509316669 2673 35-39 0.7316770186335333 2673 40-44 0.6086956521739211 2673 45-49 0.7806418219461619 2673 50-54 0.63788819875775 2673 55-59 0.7638716356107551 2673 60-64 0.8305383022774109 2673 65-69 0.8188405797101339 2673 70-74 -1.7063146997929621 2673 75-79 0.9080745341614929 2673 80-84 -4.208695652173919 2673 85-89 -1.2924430641822227 2673 90-94 0.9786749482401405 2673 95-99 -1.4285714285714377 2673 100-104 1.1663561076604338 2673 105-109 0.9813664596273242 2673 110-114 1.3407867494824046 2673 115-119 -0.8330227743271408 2673 120-124 -3.515527950310581 2673 125-129 1.9418219461697532 2673 130-134 2.479606625258775 2673 135-139 -9.541200828157368 2673 140-144 3.683643892339525 2673 145-149 1.8837474120082547 2673 150 -20.857142857142858 2676 1 0.8126293995859228 2676 2 0.9844720496894439 2676 3 0.7391304347826093 2676 4 0.950310559006212 2676 5 0.8198757763975166 2676 6 0.6045548654244328 2676 7 0.6816770186335432 2676 8 0.5093167701863379 2676 9 0.8369565217391326 2676 10-14 0.6263975155279411 2676 15-19 0.5553830227743219 2676 20-24 0.725362318840574 2676 25-29 0.6515527950310513 2676 30-34 0.6965838509316669 2676 35-39 0.7316770186335333 2676 40-44 0.6086956521739211 2676 45-49 -1.6193581780538366 2676 50-54 0.63788819875775 2676 55-59 0.7638716356107551 2676 60-64 0.8305383022774109 2676 65-69 0.8188405797101339 2676 70-74 0.6936853002070364 2676 75-79 -4.291925465838506 2676 80-84 -3.808695652173917 2676 85-89 1.107556935817776 2676 90-94 0.9786749482401405 2676 95-99 0.9714285714285609 2676 100-104 1.1663561076604338 2676 105-109 0.9813664596273242 2676 110-114 1.3407867494824046 2676 115-119 1.5669772256728578 2676 120-124 1.6844720496894183 2676 125-129 -0.45817805383024535 2676 130-134 2.479606625258775 2676 135-139 0.8587991718426338 2676 140-144 3.683643892339525 2676 145-149 -32.71625258799175 2676 150 -31.857142857142858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 6.0 13 2.0 14 3.0 15 0.0 16 0.0 17 1.0 18 0.0 19 0.0 20 1.0 21 2.0 22 7.0 23 4.0 24 6.0 25 5.0 26 3.0 27 4.0 28 7.0 29 13.0 30 17.0 31 33.0 32 54.0 33 100.0 34 193.0 35 519.0 36 2735.0 37 281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6 25.074999999999996 8.475000000000001 21.85 2 31.05 28.1 22.650000000000002 18.2 3 22.8 30.0 27.575 19.625 4 25.85 35.875 19.175 19.1 5 26.400000000000002 35.949999999999996 20.025000000000002 17.625 6 24.375 37.85 18.675 19.1 7 23.425 23.150000000000002 32.800000000000004 20.625 8 23.724999999999998 27.275 25.924999999999997 23.075000000000003 9 24.474999999999998 27.325 25.7 22.5 10-14 26.445 28.310000000000002 24.25 20.995 15-19 25.965 28.09 25.080000000000002 20.865000000000002 20-24 24.935 28.38 25.145 21.54 25-29 25.240000000000002 26.495 27.384999999999998 20.880000000000003 30-34 27.29 28.825 24.715 19.17 35-39 27.62 27.944999999999997 24.23 20.205000000000002 40-44 26.07 27.605 25.324999999999996 21.0 45-49 25.080000000000002 27.255000000000003 25.41 22.255 50-54 26.25 27.744999999999997 24.73 21.275 55-59 25.355 27.505000000000003 25.64 21.5 60-64 26.155 28.33 24.65 20.865000000000002 65-69 26.450000000000003 28.155 24.95 20.445 70-74 26.645000000000003 28.16 24.62 20.575 75-79 25.979999999999997 28.875 24.36 20.785 80-84 25.588956134647127 28.189866453258638 25.784024408542987 20.43715300355124 85-89 27.431945556445157 27.426941553242596 24.479583666933546 20.661529223378704 90-94 24.722083124687032 27.71657486229344 25.748622934401606 21.812719078617924 95-99 24.92094564071676 28.48968528836019 24.85569442353059 21.733674647392462 100-104 26.941442573510933 28.34380497612465 25.373209349082682 19.34154310128173 105-109 26.345466445149274 27.886019231737404 25.882293711926696 19.886220611186626 110-114 25.904163711883292 29.11052578259548 24.30351534798906 20.681795157532164 115-119 26.507499234771963 26.828895010713193 27.30843791449852 19.355167840016325 120-124 25.046334431630974 28.279448105436572 26.287067545304776 20.387149917627678 125-129 24.961030863556065 27.938272887872806 26.42107450898888 20.679621739582252 130-134 26.00105652403592 27.31114632857897 25.441098784997358 21.246698362387743 135-139 27.25267631394911 26.967561461079132 25.697993436978862 20.0817687879929 140-144 25.74637085729937 29.044097507532186 25.25335524513832 19.956176390030127 145-149 26.262344473581432 26.43530658929867 26.251185627406127 21.051163309713775 150 27.61447145279819 25.57942340305257 26.540418315432447 20.265686828716788 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 1.0 24 0.5 25 0.0 26 0.0 27 0.5 28 2.5 29 2.5 30 0.5 31 0.5 32 1.0 33 4.5 34 7.5 35 7.0 36 5.0 37 17.5 38 41.5 39 78.5 40 138.0 41 197.5 42 237.0 43 213.5 44 212.5 45 272.5 46 325.0 47 322.0 48 258.0 49 253.0 50 330.5 51 347.5 52 243.0 53 141.0 54 87.0 55 48.0 56 38.0 57 25.0 58 13.0 59 10.5 60 2.0 61 4.5 62 6.0 63 4.5 64 5.0 65 7.0 66 13.5 67 25.5 68 28.0 69 13.5 70 2.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 1.0 78 1.0 79 0.0 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 78-79 1.0 80-81 0.0 82-83 2.0 84-85 0.0 86-87 0.0 88-89 2.0 90-91 0.0 92-93 5.0 94-95 3.0 96-97 5.0 98-99 1.0 100-101 3.0 102-103 0.0 104-105 6.0 106-107 0.0 108-109 6.0 110-111 20.0 112-113 10.0 114-115 4.0 116-117 22.0 118-119 13.0 120-121 11.0 122-123 13.0 124-125 17.0 126-127 16.0 128-129 23.0 130-131 31.0 132-133 28.0 134-135 22.0 136-137 36.0 138-139 23.0 140-141 23.0 142-143 28.0 144-145 30.0 146-147 25.0 148-149 33.0 150-151 3538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.125 #Duplication Level Percentage of deduplicated Percentage of total 1 44.175824175824175 15.075 2 19.26739926739927 13.15 3 11.428571428571429 11.700000000000001 4 7.6190476190476195 10.4 5 4.468864468864469 7.625 6 3.0036630036630036 6.15 7 2.564102564102564 6.125 8 1.0256410256410255 2.8000000000000003 9 1.5384615384615385 4.725 >10 4.908424908424909 22.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGACC 37 0.9249999999999999 No Hit ACCATGCCGAGCCTTCCTGCCTACACTTGTCGATGTATACAACAAGATCA 24 0.6 No Hit GACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCCCT 24 0.6 No Hit GCCATGGCTTGCCCTTCCCTTGGAAGATGAAAGGAAGGCATACTTAAAGA 21 0.525 No Hit CTTCATCTCTAGCGACAGGGACCAGAGCTCCTTCGATGACTTCTTCTCTG 18 0.44999999999999996 No Hit GTGGACCATGCCGAGCCTTCCTGCCTACACTTGTCGATGTATACAACAAG 18 0.44999999999999996 No Hit ACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCCCTT 17 0.42500000000000004 No Hit GGAGGAATACAGCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCTT 17 0.42500000000000004 No Hit CCTGCCTACACTTGTCGATGTATACAACAAGATCAAGGAAAAGAATAGTG 17 0.42500000000000004 No Hit CAAGATCAAGGAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAGCG 16 0.4 No Hit AGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGAAGGGCTATGAGGTTATC 16 0.4 No Hit AGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGACCAGAGCTCCTT 15 0.375 No Hit GAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCCCTTCCCT 15 0.375 No Hit GGGAGGATTAACTATGACGAATTCTGCGCAATGATGAGAGGAGGAATACA 15 0.375 No Hit GTACCATTAGCAAACTGCTGTTGGTTCTTTTTTCCGTGGTACGTAGTTGG 15 0.375 No Hit GTTGACTCTGAGGACCTTCCCCTCAACATCTCACGTGAGACCCTTCAGCA 15 0.375 No Hit ATTAACTATGACGAATTCTGCGCAATGATGAGAGGAGGAATACAGCAGCA 15 0.375 No Hit GAGGAATACAGCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCTTT 15 0.375 No Hit CATGGCTTGCCCTTCCCTTGGAAGATGAAAGGAAGGCATACTTAAAGAAG 14 0.35000000000000003 No Hit GTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGACCAGAGCTCCTTC 14 0.35000000000000003 No Hit GAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGACCA 14 0.35000000000000003 No Hit GCTCAAGGAGTTTGAGGGCAAGAAACTTGTTTCTGCCACCAAGGAAGGCC 14 0.35000000000000003 No Hit CGGCATGGCTATTGCAACTGTGGTGACCGTTGCTGAAATTCTGAAAAATA 13 0.325 No Hit GGAAGACTGTCCTCCTTTACTTTTCAGCTCAATGGTGTGGACCATGCCGA 13 0.325 No Hit CTTGTCGATGTATACAACAAGATCAAGGAAAAGAATAGTGACTTTGAGAT 13 0.325 No Hit GGACCATGCCGAGCCTTCCTGCCTACACTTGTCGATGTATACAACAAGAT 13 0.325 No Hit GGTAAATTAGTTTTGATGTTTCCAGGGTTGTGTATTATGTACAGTAGCAC 13 0.325 No Hit GGAGAGGCTGAAGAAGAAGGGCTATGAGGTTATCTATATGGTTGACGCCA 13 0.325 No Hit AACAAGATCAAGGAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAG 13 0.325 No Hit CAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCCCTTCC 13 0.325 No Hit GTCATGGGAGGGGTTCCCCTTCAATGCAGAGAAGCTGGAAATTCTTGCTG 12 0.3 No Hit ACTACATCACTGGTGAGAGCAAGAAGGCTGTGGAGAACTCCCCCTTCCTG 12 0.3 No Hit CATATCAGAAGTTGACACGGATAACGATGGGAGGATTAACTATGACGAAT 12 0.3 No Hit CAGGGACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTG 12 0.3 No Hit GGCAAATGAAGCGGGTAAATTAGTTTTGATGTTTCCAGGGTTGTGTATTA 12 0.3 No Hit GCTCAAGTAGTCAGTCTGGTTCTTTGTAGAAACGATGTACCATTAGCAAA 12 0.3 No Hit GAATAGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGACCAGAGCT 12 0.3 No Hit AGGGACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGC 12 0.3 No Hit ATTGTCTTCATCTCTAGCGACAGGGACCAGAGCTCCTTCGATGACTTCTT 12 0.3 No Hit GGTTCTTTGTAGAAACGATGTACCATTAGCAAACTGCTGTTGGTTCTTTT 12 0.3 No Hit GAAGAAGAAGGGCTATGAGGTTATCTATATGGTTGACGCCATTGATGAGT 12 0.3 No Hit GTCTGGTTCTTTGTAGAAACGATGTACCATTAGCAAACTGCTGTTGGTTC 11 0.27499999999999997 No Hit AGCAAAGGTTCCTGTGTCACAACTTGTTGGGAAGACTGTCCTCCTTTACT 11 0.27499999999999997 No Hit GAGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGAAGGGCTATGAGGTTAT 11 0.27499999999999997 No Hit CGGATAACGATGGGAGGATTAACTATGACGAATTCTGCGCAATGATGAGA 11 0.27499999999999997 No Hit GGAGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGAAGGGCTATGAGGTTA 11 0.27499999999999997 No Hit AGAAGGCTGTGGAGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGAAGGGC 11 0.27499999999999997 No Hit CATTAGCAAACTGCTGTTGGTTCTTTTTTCCGTGGTACGTAGTTGGACGG 11 0.27499999999999997 No Hit CTGGAAACTGCTTTGATTGAGCATGAAATGGGAGACACGGATACCATAAA 11 0.27499999999999997 No Hit AAGAAGGCTGTGGAGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGAAGGG 10 0.25 No Hit GTAGAAACGATGTACCATTAGCAAACTGCTGTTGGTTCTTTTTTCCGTGG 10 0.25 No Hit AGAATAGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGACCAGAGC 10 0.25 No Hit CCTACACTTGTCGATGTATACAACAAGATCAAGGAAAAGAATAGTGACTT 10 0.25 No Hit AGAAGAAGGGCTATGAGGTTATCTATATGGTTGACGCCATTGATGAGTAT 10 0.25 No Hit GGACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCCC 10 0.25 No Hit GGGACAGAATGACATCTACTACATCACTGGTGAGAGCAAGAAGGCTGTGG 10 0.25 No Hit GCTCAATGGTGTGGACCATGCCGAGCCTTCCTGCCTACACTTGTCGATGT 10 0.25 No Hit GGAACAGAAGCATTTCAGCGTTGATGCATACAGAGTACATTCTAATCCAC 10 0.25 No Hit CTCAAGGAGTTTGAGGGCAAGAAACTTGTTTCTGCCACCAAGGAAGGCCT 10 0.25 No Hit AGGTTATCTATATGGTTGACGCCATTGATGAGTATGCCATTGGCCAGCTC 10 0.25 No Hit GTTCTTTGTAGAAACGATGTACCATTAGCAAACTGCTGTTGGTTCTTTTT 10 0.25 No Hit ATGTACAGTAGCACATGTTCCCAAGGCTGATACGGTCCGTAATCGCAACA 10 0.25 No Hit AGCACATGTTCCCAAGGCTGATACGGTCCGTAATCGCAACACTTGTTCAG 10 0.25 No Hit GCCAGCTCAAGGAGTTTGAGGGCAAGAAACTTGTTTCTGCCACCAAGGAA 10 0.25 No Hit AAGGAGTTTGAGGGCAAGAAACTTGTTTCTGCCACCAAGGAAGGCCTGAA 10 0.25 No Hit GCCATTGGCCAGCTCAAGGAGTTTGAGGGCAAGAAACTTGTTTCTGCCAC 10 0.25 No Hit AGATTGCTGAGCTGCTGAGGTACCACTCCACTAAGAGTGGTGATGAGCTG 10 0.25 No Hit GCTATTTGCAAGGCAAATGAAGCGGGTAAATTAGTTTTGATGTTTCCAGG 9 0.22499999999999998 No Hit ATGGAGCAAAGGTTCCTGTGTCACAACTTGTTGGGAAGACTGTCCTCCTT 9 0.22499999999999998 No Hit CTCGTCTTCCACCCTACTCGCGCTCCCCAAGCTACCTACCAAGTGCTGCT 9 0.22499999999999998 No Hit ATGCCATTGGCCAGCTCAAGGAGTTTGAGGGCAAGAAACTTGTTTCTGCC 9 0.22499999999999998 No Hit CCATGGCTTGCCCTTCCCTTGGAAGATGAAAGGAAGGCATACTTAAAGAA 9 0.22499999999999998 No Hit GTCAGTCTGGTTCTTTGTAGAAACGATGTACCATTAGCAAACTGCTGTTG 9 0.22499999999999998 No Hit AGATTACTGGCTTCTGGCAAATCAGTGGAACAGAGGCTGGGGCGATGATG 9 0.22499999999999998 No Hit CATAATTGAAGAGAATGGTGTGGAGTCATGGGAGGGGTTCCCCTTCAATG 9 0.22499999999999998 No Hit GAGAGGAGGAATACAGCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGT 9 0.22499999999999998 No Hit GACAGAATGACATCTACTACATCACTGGTGAGAGCAAGAAGGCTGTGGAG 9 0.22499999999999998 No Hit GGACAGAATGACATCTACTACATCACTGGTGAGAGCAAGAAGGCTGTGGA 9 0.22499999999999998 No Hit GACGAATTCTGCGCAATGATGAGAGGAGGAATACAGCAGCAGCCAGTAAG 9 0.22499999999999998 No Hit CCATTGATGAGTATGCCATTGGCCAGCTCAAGGAGTTTGAGGGCAAGAAA 9 0.22499999999999998 No Hit ATACAACAAGATCAAGGAAAAGAATAGTGACTTTGAGATTGTCTTCATCT 9 0.22499999999999998 No Hit CTTCTTCTCTGGCATGCCATGGCTTGCCCTTCCCTTGGAAGATGAAAGGA 9 0.22499999999999998 No Hit ACAAGATCAAGGAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAGC 9 0.22499999999999998 No Hit AGGAGTTTGAGGGCAAGAAACTTGTTTCTGCCACCAAGGAAGGCCTGAAG 9 0.22499999999999998 No Hit CTTTGAACAGCGGCTTGACCTCAAATGCAGTTGGAATTAGCTATTTGCAA 9 0.22499999999999998 No Hit GGAATACAGCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCTTTGT 9 0.22499999999999998 No Hit TGTACCATTAGCAAACTGCTGTTGGTTCTTTTTTCCGTGGTACGTAGTTG 9 0.22499999999999998 No Hit CTTGGAAGATGAAAGGAAGGCATACTTAAAGAAGATGTTCAAAATCCGTG 9 0.22499999999999998 No Hit CCCTGATCCAGAAGGTTGATGAGATAAAAAAAGAGGTGGATTCCATAGTT 8 0.2 No Hit GTGGAGTCATGGGAGGGGTTCCCCTTCAATGCAGAGAAGCTGGAAATTCT 8 0.2 No Hit GGCAAGGGCTAAAGCAGAGTCACAGACTTTGCAGTCCCTTCTGGTCACTG 8 0.2 No Hit CGGCTATACTTTGAACAGCGGCTTGACCTCAAATGCAGTTGGAATTAGCT 8 0.2 No Hit AATGCATAGACACAAGCTCGAAAGAGATGAGCATTTGTTCAAAGCATTCC 8 0.2 No Hit CGATGTACCATTAGCAAACTGCTGTTGGTTCTTTTTTCCGTGGTACGTAG 8 0.2 No Hit GGAAACTGCTTTGATTGAGCATGAAATGGGAGACACGGATACCATAAAGG 8 0.2 No Hit CATACTTAAAGAAGATGTTCAAAATCCGTGGGATCCCTTCTCTTGTTGCC 8 0.2 No Hit ATCTACTACATCACTGGTGAGAGCAAGAAGGCTGTGGAGAACTCCCCCTT 8 0.2 No Hit AGAAATTCGACGAACTGATGGCAGCAGCTGCAGAAGAGAGGGAGGCGGCG 8 0.2 No Hit AGTTGATCCTATCATTGGCAAGGTCATGAAGGTCGAAGGAGGGATCGTAG 8 0.2 No Hit GCTGAAGAAGAAGGGCTATGAGGTTATCTATATGGTTGACGCCATTGATG 8 0.2 No Hit AAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGACCAGA 8 0.2 No Hit CCAGCCTCAAGGACTATGTGACCAGGATGAAGGAGGGACAGAATGACATC 8 0.2 No Hit GTTATCTATATGGTTGACGCCATTGATGAGTATGCCATTGGCCAGCTCAA 7 0.17500000000000002 No Hit GGGACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCC 7 0.17500000000000002 No Hit CACTGCAAGCATAGCCCCGCAACAGAGGCGAGGCGAGCCTGTTCCGCAGG 7 0.17500000000000002 No Hit GGGCTATGAGGTTATCTATATGGTTGACGCCATTGATGAGTATGCCATTG 7 0.17500000000000002 No Hit ATGTGACCAGGATGAAGGAGGGACAGAATGACATCTACTACATCACTGGT 7 0.17500000000000002 No Hit GAATACAGCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCTTTGTA 7 0.17500000000000002 No Hit GCTAGGAACCGCCCAATTCAGAAAGCCAAGATTGAAATATTGATTGGCAA 7 0.17500000000000002 No Hit CTCTAGCGACAGGGACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGC 7 0.17500000000000002 No Hit GGAGCAAAGGTTCCTGTGTCACAACTTGTTGGGAAGACTGTCCTCCTTTA 7 0.17500000000000002 No Hit GGTTCTTTTTTCCGTGGTACGTAGTTGGACGGATAACAAGTTTACTCTCC 7 0.17500000000000002 No Hit GAAAGATGGAGCAAAGGTTCCTGTGTCACAACTTGTTGGGAAGACTGTCC 7 0.17500000000000002 No Hit GCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCCCTTCCCTTG 7 0.17500000000000002 No Hit AGGAAGTCACTGAGGCGGTGAACAACCTCAGCATAGCCGAAGGAGCGGCC 7 0.17500000000000002 No Hit GTCAACCTCGCCAAGAGGTACATGCAGCTGCACGAAGAGGTGGAGCTCTC 7 0.17500000000000002 No Hit GTTGTGTATTATGTACAGTAGCACATGTTCCCAAGGCTGATACGGTCCGT 7 0.17500000000000002 No Hit GGATGAAGGAGGGACAGAATGACATCTACTACATCACTGGTGAGAGCAAG 7 0.17500000000000002 No Hit AGGAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGGAC 7 0.17500000000000002 No Hit CGTACTTAAAGAAGATGTTCAAAATCCGTGGGATCCCTTCTCTTGTTGCC 7 0.17500000000000002 No Hit GTACAAATGAGCCTTCCGATGGAGATGATGGTGATTCCACAGGGGTTAGT 7 0.17500000000000002 No Hit CTATTTGCAAGGCAAATGAAGCGGGTAAATTAGTTTTGATGTTTCCAGGG 7 0.17500000000000002 No Hit GCAAGGGCTAAAGCAGAGTCACAGACTTTGCAGTCCCTTCTGGTCACTGG 7 0.17500000000000002 No Hit ATGAGGTTATCTATATGGTTGACGCCATTGATGAGTATGCCATTGGCCAG 7 0.17500000000000002 No Hit GTTACTTTGAGCTTAATGATCTTCCTACACTTGTGCTGATTGGTCCTGAT 7 0.17500000000000002 No Hit GAAAGGAAGGCATACTTAAAGAAGATGTTCAAAATCCGTGGGATCCCTTC 7 0.17500000000000002 No Hit GGACTATGTGACCAGGATGAAGGAGGGACAGAATGACATCTACTACATCA 7 0.17500000000000002 No Hit CAAAGATGATGCTAGGAACCGCCCAATTCAGAAAGCCAAGATTGAAATAT 7 0.17500000000000002 No Hit GGAGGATTAACTATGACGAATTCTGCGCAATGATGAGAGGAGGAATACAG 7 0.17500000000000002 No Hit GTTGCTGGTATGCCCTCGACAAAGAACATGGCCAGAAACTACGACGACGC 7 0.17500000000000002 No Hit GTCCCTTCTGGTCACTGGTGATTTAGACTTTGTCATTGGGAAAGATGGAG 7 0.17500000000000002 No Hit GAAATTCTTGCTGAGAAGGCAAGGGCTAAAGCAGAGTCACAGACTTTGCA 7 0.17500000000000002 No Hit TCTAGCGACAGGGACCAGAGCTCCTTCGATGACTTCTTCTCTGGCATGCC 7 0.17500000000000002 No Hit GTCGAAGGAGGGATCGTAGAGAAAGAAGGTGATAAATAGAAACAAACTGG 7 0.17500000000000002 No Hit ATTATGTACAGTAGCACATGTTCCCAAGGCTGATACGGTCCGTAATCGCA 7 0.17500000000000002 No Hit GATGGGAGGATTAACTATGACGAATTCTGCGCAATGATGAGAGGAGGAAT 7 0.17500000000000002 No Hit GCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCTTTGTAGAAACGATGTACC 7 0.17500000000000002 No Hit AGGACATCATATCAGAAGTTGACACGGATAACGATGGGAGGATTAACTAT 6 0.15 No Hit GTCCTCCTTTACTTTTCAGCTCAATGGTGTGGACCATGCCGAGCCTTCCT 6 0.15 No Hit TCCTGCCTACACTTGTCGATGTATACAACAAGATCAAGGAAAAGAATAGT 6 0.15 No Hit GGAAGGCATACTTAAAGAAGATGTTCAAAATCCGTGGGATCCCTTCTCTT 6 0.15 No Hit GGCGTACTTAAAGAAGATGTTCAAAATCCGTGGGATCCCTTCTCTTGTTG 6 0.15 No Hit AAGAAGGGCTATGAGGTTATCTATATGGTTGACGCCATTGATGAGTATGC 6 0.15 No Hit GCAAGAAGGCTGTGGAGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGAAG 6 0.15 No Hit CACTGAGGCGGTGAACAACCTCAGCATAGCCGAAGGAGCGGCCGGAGCAG 6 0.15 No Hit GTGACCAGGATGAAGGAGGGACAGAATGACATCTACTACATCACTGGTGA 6 0.15 No Hit TCTCTTTGCCTGAGGTGGAGGCGCTAGTGGACGCTGTCGAACAGCTTGGG 6 0.15 No Hit GCCATGGCTTGCCCTTCCCTTGGAAGATGAAAGGAAGGCGTACTTAAAGA 6 0.15 No Hit CAGCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCTTTGTAGAAAC 6 0.15 No Hit TGAGTATGCCATTGGCCAGCTCAAGGAGTTTGAGGGCAAGAAACTTGTTT 6 0.15 No Hit CGGATACCATAAAGGACATCATATCAGAAGTTGACACGGATAACGATGGG 6 0.15 No Hit GGATTAACTATGACGAATTCTGCGCAATGATGAGAGGAGGAATACAGCAG 6 0.15 No Hit CTTTGATTGAGCATGAAATGGGAGACACGGATACCATAAAGGACATCATA 6 0.15 No Hit GAGGGACAGAATGACATCTACTACATCACTGGTGAGAGCAAGAAGGCTGT 6 0.15 No Hit CATGAAGGTCGAAGGAGGGATCGTAGAGAAAGAAGGTGATAAATAGAAAC 6 0.15 No Hit GGTGAATCTGTTGAGTCGACTGCAGATGGAGATGATGGTGACTCCACAGG 6 0.15 No Hit GCCGCCGAAATCTCTCGTCTTCCACCCTACTCGCGCTCCCCAAGCTACCT 6 0.15 No Hit AGGAGGAATACAGCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCT 6 0.15 No Hit GAAGATGAAAGGAAGGCATACTTAAAGAAGATGTTCAAAATCCGTGGGAT 6 0.15 No Hit GAGCAAGAAGGCTGTGGAGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGA 6 0.15 No Hit GTCTTCATCTCTAGCGACAGGGACCAGAGCTCCTTCGATGACTTCTTCTC 6 0.15 No Hit GAATGAATGTGGAATTGAAGAAGACGTTGTTGCTGGTATGCCCTCGACAA 6 0.15 No Hit GGATGGGGAACCAGTGATGCTGGGGAAGATTACTGGCTTCTGGCAAATCA 6 0.15 No Hit GCAGCAGCCAGTAAGGCTCAAGTAGTCAGTCTGGTTCTTTGTAGAAACGA 6 0.15 No Hit CGCCATTGATGAGTATGCCATTGGCCAGCTCAAGGAGTTTGAGGGCAAGA 6 0.15 No Hit AGAAGGGCTATGAGGTTATCTATATGGTTGACGCCATTGATGAGTATGCC 6 0.15 No Hit AAGATGTGTCAGAAGCTGGTCAGTTACTTTGAGCTTAATGATCTTCCTAC 6 0.15 No Hit TCAAGGACTATGTGACCAGGATGAAGGAGGGACAGAATGACATCTACTAC 6 0.15 No Hit GCTTCATTACAAGAGATGAACTGGAAACTGCTTTGATTGAGCATGAAATG 6 0.15 No Hit GCCGAAATCTCTCGTCTTCCACCCTACTCGCGCTCCCCAAGCTACCTACC 6 0.15 No Hit TGTGGACCATGCCGAGCCTTCCTGCCTACACTTGTCGATGTATACAACAA 6 0.15 No Hit AGAGCTCCTTCGATGACTTCTTCTCTGGCATGCCATGGCTTGCCCTTCCC 6 0.15 No Hit GAGCTGCTGAGGTACCACTCCACTAAGAGTGGTGATGAGCTGACCAGCCT 6 0.15 No Hit GAGCTGACCAGCCTCAAGGACTATGTGACCAGGATGAAGGAGGGACAGAA 6 0.15 No Hit GTAAGGCTCAAGTAGTCAGTCTGGTTCTTTGTAGAAACGATGTACCATTA 6 0.15 No Hit CAAGGACTATGTGACCAGGATGAAGGAGGGACAGAATGACATCTACTACA 6 0.15 No Hit CTTTTTTCCGTGGTACGTAGTTGGACGGATAACAAGTTTACTCTCCAAGT 6 0.15 No Hit GGTGACTCCACAGGCGTTACTATTATGAGTCGACGCCCTGCTCCAACTAC 6 0.15 No Hit GACACGGATACCATAAAGGACATCATATCAGAAGTTGACACGGATAACGA 5 0.125 No Hit GAGGTACCACTCCACTAAGAGTGGTGATGAGCTGACCAGCCTCAAGGACT 5 0.125 No Hit GTATGCCATTGGCCAGCTCAAGGAGTTTGAGGGCAAGAAACTTGTTTCTG 5 0.125 No Hit ATTAGCAAACTGCTGTTGGTTCTTTTTTCCGTGGTACGTAGTTGGACGGA 5 0.125 No Hit GGAAGGCGTACTTAAAGAAGATGTTCAAAATCCGTGGGATCCCTTCTCTT 5 0.125 No Hit AGCAAGAAGGCTGTGGAGAACTCCCCCTTCCTGGAGAGGCTGAAGAAGAA 5 0.125 No Hit CCTTAAACTTGGTATCCATGAAGACTCTGCCAACAGGACCAAGATTGCTG 5 0.125 No Hit CAAGTTTACTCTCCAAGTCTGCTTTTGTATTGCTGAGATGATGAATTGAT 5 0.125 No Hit AGATCATAAGGGGCAAGAATGAATGTGGAATTGAAGAAGACGTTGTTGCT 5 0.125 No Hit TCTCTGGCATGCCATGGCTTGCCCTTCCCTTGGAAGATGAAAGGAAGGCA 5 0.125 No Hit AAACAATTCAGAGCTATGAATAAGCTGAAAAAGATGGCTCTGAAGGTTAT 5 0.125 No Hit ATCATTGGCAAGGTCATGAAGGTCGAAGGAGGGATCGTAGAGAAAGAAGG 5 0.125 No Hit GTCAAAGATGATGCTAGGAACCGCCCAATTCAGAAAGCCAAGATTGAAAT 5 0.125 No Hit ATACAGAGTACATTCTAATCCACATGACATCATGGCAGAGGTCTATACTA 5 0.125 No Hit GTTCATCACAGCCACAATGCATAGACACAAGCTCGAAAGAGATGAGCATT 5 0.125 No Hit GTTGGACGGATAACAAGTTTACTCTCCAAGTCTGCTTTTGTATTGCTGAG 5 0.125 No Hit GGCTCAAGTAGTCAGTCTGGTTCTTTGTAGAAACGATGTACCATTAGCAA 5 0.125 No Hit AGAAAGCCAAGATTGAAATATTGATTGGCAAGACGGAGAAATTCGACGAA 5 0.125 No Hit GTACAGTAGCACATGTTCCCAAGGCTGATACGGTCCGTAATCGCAACACT 5 0.125 No Hit CAGAAGCTGAAGTAAAGCAGTTGATGGATGCCGCTGATGTGGACGGCAGT 5 0.125 No Hit CTACATCACTGGTGAGAGCAAGAAGGCTGTGGAGAACTCCCCCTTCCTGG 5 0.125 No Hit CAAGGAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTAGCGACAGGG 5 0.125 No Hit CCTTGGAAGATGAAAGGAAGGCATACTTAAAGAAGATGTTCAAAATCCGT 5 0.125 No Hit GATCAAGGGCTTGAAACAAATGTTCAGTAACATGGACACAGACAACAGTG 5 0.125 No Hit GCATACTTAAAGAAGATGTTCAAAATCCGTGGGATCCCTTCTCTTGTTGC 5 0.125 No Hit CAGAAAGCCAAGATTGAAATATTGATTGGCAAGACGGAGAAATTCGACGA 5 0.125 No Hit TATGAGGTTATCTATATGGTTGACGCCATTGATGAGTATGCCATTGGCCA 5 0.125 No Hit GGCTGAAGAAGAAGGGCTATGAGGTTATCTATATGGTTGACGCCATTGAT 5 0.125 No Hit AGTAAGGCTCAAGTAGTCAGTCTGGTTCTTTGTAGAAACGATGTACCATT 5 0.125 No Hit CATGACATCAACTGTTGATGTCAAAGATGATGCTAGGAACCGCCCAATTC 5 0.125 No Hit ATTAGCTATTTGCAAGGCAAATGAAGCGGGTAAATTAGTTTTGATGTTTC 5 0.125 No Hit GCACAAAGGCGCATAAGGAGGCCATTCTCTTTGCCTGAGGTGGAGGCGCT 5 0.125 No Hit AGGGATCGTAGAGAAAGAAGGTGATAAATAGAAACAAACTGGATGCTTAC 5 0.125 No Hit GACTGTCCTCCTTTACTTTTCAGCTCAATGGTGTGGACCATGCCGAGCCT 5 0.125 No Hit GCCAGAAACTACGACGACGCCTTTGGAACGGCTATACTTTGAACAGCGGC 5 0.125 No Hit CATGAAGACTCTGCCAACAGGACCAAGATTGCTGAGCTGCTGAGGTACCA 5 0.125 No Hit CTTGCCCTTCCCTTGGAAGATGAAAGGAAGGCATACTTAAAGAAGATGTT 5 0.125 No Hit TAGAAACGATGTACCATTAGCAAACTGCTGTTGGTTCTTTTTTCCGTGGT 5 0.125 No Hit GCACAAAGGAGGTTACGGCTACAAGATTCATTGTCTGCTGTAAATATTGA 5 0.125 No Hit AGAATGAATGTGGAATTGAAGAAGACGTTGTTGCTGGTATGCCCTCGACA 5 0.125 No Hit GTGGGACAATCACATACGAAGAACTCAAAGCAGGATTGGCCAAACTTGGA 5 0.125 No Hit GAAATCTCTCGTCTTCCACCCTACTCGCGCTCCCCAAGCTACCTACCAAG 5 0.125 No Hit GTGTGGACCATGCCGAGCCTTCCTGCCTACACTTGTCGATGTATACAACA 5 0.125 No Hit ATTTGTTCAAAGCATTCCAGTATTTTGACAAAGACAGTAGTGGCTTCATT 5 0.125 No Hit CAAGCTCGAAAGAGATGAGCATTTGTTCAAAGCATTCCAGTATTTTGACA 5 0.125 No Hit AAATTAGTTTTGATGTTTCCAGGGTTGTGTATTATGTACAGTAGCACATG 5 0.125 No Hit GCCTCAAGGACTATGTGACCAGGATGAAGGAGGGACAGAATGACATCTAC 5 0.125 No Hit GAGGTTATCTATATGGTTGACGCCATTGATGAGTATGCCATTGGCCAGCT 5 0.125 No Hit TGTTGGTTCTTTTTTCCGTGGTACGTAGTTGGACGGATAACAAGTTTACT 5 0.125 No Hit GGAAAATGCAGAATTTGAGGCCATTGCCTTTCTTCGTTGGAAGTTTGCGG 5 0.125 No Hit GAGAGGCTGAAGAAGAAGGGCTATGAGGTTATCTATATGGTTGACGCCAT 5 0.125 No Hit CGGGTAAATTAGTTTTGATGTTTCCAGGGTTGTGTATTATGTACAGTAGC 5 0.125 No Hit GCAAAGGTTCCTGTGTCACAACTTGTTGGGAAGACTGTCCTCCTTTACTT 5 0.125 No Hit AAGTAGTCAGTCTGGTTCTTTGTAGAAACGATGTACCATTAGCAAACTGC 5 0.125 No Hit CATCTCTAGCGACAGGGACCAGAGCTCCTTCGATGACTTCTTCTCTGGCA 5 0.125 No Hit GCAGAACAAGATCCTCAAGGTCATCCGCAAGAACCTTGTCAAGAAGTCCA 5 0.125 No Hit AGCTGACCAGCCTCAAGGACTATGTGACCAGGATGAAGGAGGGACAGAAT 5 0.125 No Hit CACGGATAACGATGGGAGGATTAACTATGACGAATTCTGCGCAATGATGA 5 0.125 No Hit CGGATAACAAGTTTACTCTCCAAGTCTGCTTTTGTATTGCTGAGATGATG 5 0.125 No Hit GAATTCTGCGCAATGATGAGAGGAGGAATACAGCAGCAGCCAGTAAGGCT 5 0.125 No Hit CAACAAGATCAAGGAAAAGAATAGTGACTTTGAGATTGTCTTCATCTCTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGATGC 30 0.0028022358 33.66819 115-119 >>END_MODULE Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531776 spots for ERR5262805.sra Written 1531776 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra Read 1531763 spots for ERR5262805.sra Written 1531763 spots for ERR5262805.sra SRR ids: ['ERR5262805.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6v1yxliv ERR5262805.sra spots: 30635273 blocks: [[1, 1531763], [1531764, 3063526], [3063527, 4595289], [4595290, 6127052], [6127053, 7658815], [7658816, 9190578], [9190579, 10722341], [10722342, 12254104], [12254105, 13785867], [13785868, 15317630], [15317631, 16849393], [16849394, 18381156], [18381157, 19912919], [19912920, 21444682], [21444683, 22976445], [22976446, 24508208], [24508209, 26039971], [26039972, 27571734], [27571735, 29103497], [29103498, 30635273]] ERR5262805 file size 10948591 ERR5262805 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262805 ERR5262805_1.fastq ERR5262805_2.fastq Input file: ERR5262805_1.fastq Paired file: ERR5262805_2.fastq trimmed: ERR5262805-trimmed-pair1.fastq, ERR5262805-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:13:03 2024 >> started Fri Dec 6 12:13:57 2024 >> done (53.139s) 30635273 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 30635273 (100.00%) read pairs available; of these: 8793 ( 0.03%) trimmed read pairs available after processing 30626480 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 2 0.00% 24 1 0.00% 25 2 0.00% 26 0 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 3 0.00% 31 0 0.00% 32 0 0.00% 33 1 0.00% 34 5 0.00% 35 2 0.00% 36 2 0.00% 37 3 0.00% 38 3 0.00% 39 7 0.00% 40 2 0.00% 41 3 0.00% 42 1 0.00% 43 0 0.00% 44 1 0.00% 45 3 0.00% 46 3 0.00% 47 0 0.00% 48 4 0.00% 49 340 0.00% 50 382 0.00% 51 400 0.00% 52 475 0.00% 53 482 0.00% 54 457 0.00% 55 567 0.00% 56 607 0.00% 57 732 0.00% 58 822 0.00% 59 1005 0.00% 60 1061 0.00% 61 1189 0.00% 62 1375 0.00% 63 1535 0.01% 64 1803 0.01% 65 1860 0.01% 66 2126 0.01% 67 2379 0.01% 68 2655 0.01% 69 2990 0.01% 70 3464 0.01% 71 4039 0.01% 72 4589 0.01% 73 5153 0.02% 74 5839 0.02% 75 6288 0.02% 76 6934 0.02% 77 7665 0.03% 78 8566 0.03% 79 9573 0.03% 80 10612 0.03% 81 11984 0.04% 82 13352 0.04% 83 15233 0.05% 84 16729 0.05% 85 18130 0.06% 86 19485 0.06% 87 21068 0.07% 88 22555 0.07% 89 24137 0.08% 90 25669 0.08% 91 28186 0.09% 92 30828 0.10% 93 32939 0.11% 94 35482 0.12% 95 37688 0.12% 96 38919 0.13% 97 41064 0.13% 98 42912 0.14% 99 44145 0.14% 100 46531 0.15% 101 48992 0.16% 102 51606 0.17% 103 53904 0.18% 104 56374 0.18% 105 59531 0.19% 106 61466 0.20% 107 62974 0.21% 108 64563 0.21% 109 66932 0.22% 110 68201 0.22% 111 69934 0.23% 112 73499 0.24% 113 74698 0.24% 114 78753 0.26% 115 80244 0.26% 116 82898 0.27% 117 84159 0.27% 118 86611 0.28% 119 86712 0.28% 120 87653 0.29% 121 89561 0.29% 122 90725 0.30% 123 93072 0.30% 124 96785 0.32% 125 97922 0.32% 126 100860 0.33% 127 101410 0.33% 128 102340 0.33% 129 103856 0.34% 130 104526 0.34% 131 106039 0.35% 132 106937 0.35% 133 108025 0.35% 134 110673 0.36% 135 113470 0.37% 136 115081 0.38% 137 115570 0.38% 138 116805 0.38% 139 118686 0.39% 140 120160 0.39% 141 123658 0.40% 142 125504 0.41% 143 125995 0.41% 144 128256 0.42% 145 126991 0.41% 146 131527 0.43% 147 259729 0.85% 148 121356 0.40% 149 121185 0.40% 150 25193816 82.24% 30635273 reads passed initial QC criterion=sequence-density sequence-density=1.07 sequence-density-rank=1 fanout-score=3.54 fanout-score-rank=20 prefix-density=1.33 prefix-fanout=2.8 sequence=TCGGCGCACCGG criterion=fanout-score sequence-density=0.04 sequence-density-rank=29 fanout-score=66.86 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=3.1 sequence=CCGCCGCCGCCTCCTCCGCCACGACCGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAAC criterion=sequence-density sequence-density=8.23 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=35 prefix-density=8.19 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=290.50 fanout-score-rank=1 prefix-density=1.89 prefix-fanout=1.0 sequence=CAAAACACAACACATTCTAGCTTTCGAAGTAGAGGTACATATTATATATACACATATATACAGATTGGGGATCAGTGACCATGAAGAGCAG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TCGGCGCACCGG -y CGGTTCCGGTTC -o ERR5262805 ERR5262805_1.fastq ERR5262805_2.fastq Input file: ERR5262805_1.fastq Paired file: ERR5262805_2.fastq trimmed: ERR5262805-trimmed-pair1.fastq, ERR5262805-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TCGGCGCACCGG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:18:27 2024 >> started Fri Dec 6 12:18:49 2024 >> done (22.474s) 20423515 read pairs processed; of these: 749 ( 0.00%) short read pairs filtered out after trimming by size control 442 ( 0.00%) empty read pairs filtered out after trimming by size control 20422324 (99.99%) read pairs available; of these: 170 ( 0.00%) trimmed read pairs available after processing 20422154 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 1 0.00% 25 2 0.00% 26 0 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 3 0.00% 31 0 0.00% 32 0 0.00% 33 0 0.00% 34 5 0.00% 35 1 0.00% 36 1 0.00% 37 2 0.00% 38 1 0.00% 39 5 0.00% 40 2 0.00% 41 0 0.00% 42 1 0.00% 43 0 0.00% 44 1 0.00% 45 2 0.00% 46 1 0.00% 47 0 0.00% 48 2 0.00% 49 247 0.00% 50 299 0.00% 51 322 0.00% 52 360 0.00% 53 361 0.00% 54 330 0.00% 55 417 0.00% 56 468 0.00% 57 557 0.00% 58 616 0.00% 59 776 0.00% 60 793 0.00% 61 929 0.00% 62 1043 0.01% 63 1215 0.01% 64 1357 0.01% 65 1231 0.01% 66 1065 0.01% 67 1095 0.01% 68 1647 0.01% 69 2323 0.01% 70 2598 0.01% 71 3004 0.01% 72 3456 0.02% 73 3935 0.02% 74 2747 0.01% 75 4106 0.02% 76 4920 0.02% 77 4012 0.02% 78 5510 0.03% 79 6211 0.03% 80 7003 0.03% 81 7594 0.04% 82 8207 0.04% 83 9304 0.05% 84 11536 0.06% 85 13084 0.06% 86 11553 0.06% 87 13513 0.07% 88 16462 0.08% 89 16348 0.08% 90 16139 0.08% 91 18874 0.09% 92 19374 0.09% 93 21942 0.11% 94 22911 0.11% 95 24504 0.12% 96 27319 0.13% 97 28528 0.14% 98 27832 0.14% 99 27757 0.14% 100 30978 0.15% 101 34917 0.17% 102 34328 0.17% 103 36320 0.18% 104 36219 0.18% 105 40321 0.20% 106 40494 0.20% 107 43131 0.21% 108 42527 0.21% 109 44652 0.22% 110 43695 0.21% 111 47364 0.23% 112 48421 0.24% 113 49741 0.24% 114 52357 0.26% 115 54550 0.27% 116 54714 0.27% 117 57450 0.28% 118 58009 0.28% 119 56642 0.28% 120 58873 0.29% 121 58819 0.29% 122 60312 0.30% 123 61666 0.30% 124 64458 0.32% 125 65241 0.32% 126 67157 0.33% 127 67652 0.33% 128 67954 0.33% 129 69027 0.34% 130 69764 0.34% 131 71006 0.35% 132 72304 0.35% 133 72406 0.35% 134 74046 0.36% 135 76134 0.37% 136 76942 0.38% 137 77270 0.38% 138 77593 0.38% 139 79712 0.39% 140 80431 0.39% 141 83023 0.41% 142 83725 0.41% 143 84266 0.41% 144 85768 0.42% 145 84616 0.41% 146 88033 0.43% 147 172563 0.84% 148 81249 0.40% 149 80660 0.39% 150 16795058 82.24% criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=4.45 fanout-score-rank=32 prefix-density=0.31 prefix-fanout=3.3 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.04 sequence-density-rank=35 fanout-score=252.10 fanout-score-rank=1 prefix-density=0.54 prefix-fanout=17.3 sequence=GCCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTG criterion=sequence-density sequence-density=0.86 sequence-density-rank=1 fanout-score=2.04 fanout-score-rank=38 prefix-density=0.87 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.05 sequence-density-rank=32 fanout-score=468.30 fanout-score-rank=1 prefix-density=1.12 prefix-fanout=19.0 sequence=CCGCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTACGCGTGGGTGCTTGACAAGCTGAAGGCTGAGCGTGAGAGAGGTATCACCATCGATATTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGATGCCCCTGGACACCGTGACTTCATCAAGAACATGATTACCGGTACCTCCCAGGCTGACTGTGCCGTGCTTATCATTGACTCCACGACTGGAGGTTTTGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCATGCCCTCCTTGCTTTCACTCTTGGAGTGAAGCAGAT ERR5262805 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:20:08 Started mapping on | Dec 06 12:20:08 Finished on | Dec 06 12:22:36 Mapping speed, Million of reads per hour | 745.15 Number of input reads | 30634082 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 29530864 Uniquely mapped reads % | 96.40% Average mapped length | 289.87 Number of splices: Total | 27614877 Number of splices: Annotated (sjdb) | 25688645 Number of splices: GT/AG | 27206806 Number of splices: GC/AG | 342192 Number of splices: AT/AC | 18271 Number of splices: Non-canonical | 47608 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.13 Insertion rate per base | 0.01% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 343720 % of reads mapped to multiple loci | 1.12% Number of reads mapped to too many loci | 1306 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.45% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 759498 759498 759498 N_multimapping 343720 343720 343720 N_noFeature 1038289 28689522 1286996 N_ambiguous 688519 4129 96401 UnstrandedReadsAssigned:27804056 PositiveStrandReadsAssigned:837213 NegativeStrandReadsAssigned:28147467 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262805 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262805-trimmed-pair1.fastq ERR5262805-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,634,082 reads, 28,548,169 reads pseudoaligned [quant] estimated average fragment length: 257.842 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,184 rounds 52973 ERR5262805.ke.tsv 35125 ERR5262805.se.tsv 88098 total ==> ERR5262805.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 679.71 0 0 PNS24247 1044 787.158 178.283 11.0051 PNS24249 1928 1671.16 315.81 9.18239 PNS24246 1044 787.158 178.283 11.0051 PNS24248 1044 787.158 178.283 11.0051 PNS24244 1471 1214.16 353.342 14.1406 PNS24243 293 105.66 3 1.37961 KQK14069 1603 1346.16 68424.3 2469.8 KQK14071 474 243.533 1028.74 205.256 ==> ERR5262805.se.tsv <== BRADI_1g14170v3 72730 BRADI_1g53295v3 307 BRADI_1g59795v3 815 BRADI_1g07683v3 0 BRADI_1g00485v3 6 BRADI_1g20270v3 727 BRADI_1g74790v3 2953 BRADI_1g09890v3 0 BRADI_1g77505v3 524 BRADI_1g48960v3 0 ERR5262805 completed mapping pipeline successfully