Starting /dee2/code/volunteer_pipeline.sh ERR5262806 current disk space = 1551206608896 free memory = 1600350632 ERR5262806 SRAfilesize 26645c5c766a99cea8fe89519d97488f ERR5262806.sra ERR5262806.sra file validated ERR5262806 is paired end ERR5262806 is conventional basespace ERR5262806 read1 length is 65-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262806_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 65-150 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5195 37.0 37.0 37.0 37.0 37.0 2 36.44475 37.0 37.0 37.0 37.0 37.0 3 36.557 37.0 37.0 37.0 37.0 37.0 4 36.6105 37.0 37.0 37.0 37.0 37.0 5 36.589 37.0 37.0 37.0 37.0 37.0 6 36.609 37.0 37.0 37.0 37.0 37.0 7 36.5475 37.0 37.0 37.0 37.0 37.0 8 36.633 37.0 37.0 37.0 37.0 37.0 9 36.603 37.0 37.0 37.0 37.0 37.0 10-14 36.5581 37.0 37.0 37.0 37.0 37.0 15-19 36.4825 37.0 37.0 37.0 37.0 37.0 20-24 36.4508 37.0 37.0 37.0 37.0 37.0 25-29 36.4123 37.0 37.0 37.0 37.0 37.0 30-34 36.3852 37.0 37.0 37.0 37.0 37.0 35-39 36.3251 37.0 37.0 37.0 37.0 37.0 40-44 36.326800000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.243700000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.25840000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.1205 37.0 37.0 37.0 37.0 37.0 60-64 36.0955 37.0 37.0 37.0 37.0 37.0 65-69 36.03240377594399 37.0 37.0 37.0 37.0 37.0 70-74 36.06256876953789 37.0 37.0 37.0 37.0 37.0 75-79 36.025375237634435 37.0 37.0 37.0 37.0 37.0 80-84 36.08855240722575 37.0 37.0 37.0 37.0 37.0 85-89 35.918127325055124 37.0 37.0 37.0 37.0 37.0 90-94 35.89336055569786 37.0 37.0 37.0 37.0 37.0 95-99 35.88178268462158 37.0 37.0 37.0 37.0 37.0 100-104 35.80386999747901 37.0 37.0 37.0 37.0 37.0 105-109 35.82793188673464 37.0 37.0 37.0 37.0 37.0 110-114 35.81802246461374 37.0 37.0 37.0 37.0 37.0 115-119 35.763511869729726 37.0 37.0 37.0 37.0 37.0 120-124 35.64135708917627 37.0 37.0 37.0 37.0 37.0 125-129 35.673212524650786 37.0 37.0 37.0 37.0 37.0 130-134 35.61713878127561 37.0 37.0 37.0 37.0 37.0 135-139 35.56645194345059 37.0 37.0 37.0 37.0 37.0 140-144 35.49149188873831 37.0 37.0 37.0 37.0 37.0 145-149 35.41392749613634 37.0 37.0 37.0 37.0 37.0 150 35.20283155157469 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 2.0 21 3.0 22 7.0 23 3.0 24 4.0 25 10.0 26 8.0 27 13.0 28 22.0 29 36.0 30 34.0 31 44.0 32 57.0 33 78.0 34 129.0 35 339.0 36 2773.0 37 437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.949999999999996 14.825 12.45 30.775000000000002 2 22.88072018004501 12.728182045511376 38.234558639659916 26.156539134783696 3 20.45 23.275000000000002 31.2 25.074999999999996 4 23.674999999999997 25.174999999999997 25.7 25.45 5 23.474999999999998 31.424999999999997 27.925 17.175 6 17.325 36.8 28.075 17.8 7 14.75 28.725 41.349999999999994 15.174999999999999 8 15.325 27.575 32.7 24.4 9 16.55 23.375 35.75 24.325 10-14 19.575 30.755 26.605 23.064999999999998 15-19 21.09 29.98 26.090000000000003 22.84 20-24 20.48 30.044999999999998 24.6 24.875 25-29 23.1 29.494999999999997 25.085 22.32 30-34 20.095 29.565 26.325 24.015 35-39 20.74 30.599999999999998 25.650000000000002 23.01 40-44 21.34 30.885 25.580000000000002 22.195 45-49 21.125 29.909999999999997 26.340000000000003 22.625 50-54 23.93 28.315 24.705 23.05 55-59 19.475 32.269999999999996 25.580000000000002 22.675 60-64 19.165 33.650000000000006 24.905 22.28 65-69 17.578515703140628 32.30146029205841 26.405281056211244 23.714742948589716 70-74 20.23208122842995 32.531385985094786 24.64862701945681 22.587905767018455 75-79 19.753766077773886 32.40578549622141 25.409138681747663 22.431309744257042 80-84 19.712669570005506 33.35335635981379 25.309105471291986 21.62486859888872 85-89 19.262931250312953 33.047919483250716 24.911121125632167 22.778028140804167 90-94 18.0565300190438 36.50897063245465 25.558785205973738 19.875714142527816 95-99 20.14640994785399 33.950060168471715 27.702567188126753 18.20096269554753 100-104 20.345450893753767 32.958425386623816 26.556537457320744 20.139586262301666 105-109 21.61441296361532 35.59458507372553 24.66408333752705 18.126918625132102 110-114 20.999191755910285 33.870478884623154 25.646595271772078 19.483734087694486 115-119 18.694270779952856 37.045198319155475 24.57210208055755 19.68842882033412 120-124 19.166926029671128 34.95694574125947 26.68845315904139 19.187675070028014 125-129 18.96506297096485 33.640722980450015 28.75059282289087 18.64362122569426 130-134 19.736489743452413 33.05125595843822 26.313534358095442 20.898719940013923 135-139 20.373801220575412 30.78683522231909 28.37837837837838 20.460985178727114 140-144 18.782094217459257 31.887698146907788 26.144228622460368 23.185979013172584 145-149 19.776887871853546 31.12128146453089 28.066361556064074 21.03546910755149 150 18.318405085235483 32.302802658191275 26.495232591736496 22.883559664836753 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.5 14 2.0 15 1.0 16 0.5 17 2.0 18 2.0 19 0.5 20 0.5 21 2.0 22 6.0 23 6.5 24 5.0 25 6.0 26 6.5 27 30.0 28 38.0 29 32.0 30 53.0 31 102.5 32 171.5 33 175.5 34 141.5 35 126.0 36 96.0 37 84.0 38 89.5 39 90.5 40 109.0 41 177.5 42 266.5 43 269.0 44 247.5 45 220.5 46 167.5 47 152.0 48 199.5 49 207.5 50 146.5 51 81.0 52 40.5 53 39.5 54 27.5 55 22.0 56 24.5 57 23.5 58 16.0 59 7.5 60 4.0 61 8.0 62 20.5 63 27.5 64 34.5 65 34.0 66 29.0 67 17.5 68 14.0 69 18.5 70 17.5 71 15.0 72 7.5 73 12.0 74 11.0 75 6.0 76 3.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 64-65 1.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.0 74-75 1.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 0.0 84-85 0.0 86-87 1.0 88-89 2.0 90-91 2.0 92-93 0.0 94-95 0.0 96-97 2.0 98-99 2.0 100-101 2.0 102-103 5.0 104-105 2.0 106-107 6.0 108-109 1.0 110-111 4.0 112-113 27.0 114-115 22.0 116-117 26.0 118-119 21.0 120-121 11.0 122-123 21.0 124-125 29.0 126-127 28.0 128-129 24.0 130-131 25.0 132-133 18.0 134-135 27.0 136-137 29.0 138-139 37.0 140-141 41.0 142-143 33.0 144-145 38.0 146-147 26.0 148-149 22.0 150-151 3461.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.175 #Duplication Level Percentage of deduplicated Percentage of total 1 50.31982942430704 17.7 2 19.758351101634684 13.900000000000002 3 10.874200426439232 11.475 4 5.899076048329779 8.3 5 2.6297085998578535 4.625 6 2.345415778251599 4.95 7 1.2082444918265813 2.9749999999999996 8 1.5636105188343994 4.3999999999999995 9 1.0660980810234542 3.375 >10 4.193319118692253 25.7 >50 0.14214641080312723 2.6 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTAGCTCTACTAGATCTGCACACAGGGAAGTGCAAACTCTACAGGTTTT 53 1.325 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 51 1.275 No Hit GTAGTATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCTATAAA 43 1.075 No Hit GGTAGAACAAAAACCTGCTGCTCCAATTTACCATACGCAGCACCTGAATT 41 1.0250000000000001 No Hit AGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 39 0.975 No Hit ATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTAT 37 0.9249999999999999 No Hit CCAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGC 35 0.8750000000000001 No Hit GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 34 0.8500000000000001 No Hit GACCATTACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAA 33 0.8250000000000001 No Hit CTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTACAAGGG 29 0.7250000000000001 No Hit CATTACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAATGG 29 0.7250000000000001 No Hit ACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGT 27 0.675 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 26 0.65 No Hit GTGGAAACATCTTGTAATCTTTTTGCATAAAAAAGCCCTGGCATTCCTCC 25 0.625 No Hit CCATTACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAATG 24 0.6 No Hit GTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCTTCACGCA 20 0.5 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 20 0.5 No Hit CTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATG 19 0.475 No Hit CACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCG 18 0.44999999999999996 No Hit CTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGC 17 0.42500000000000004 No Hit GGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGC 17 0.42500000000000004 No Hit GTAGAACAAAAACCTGCTGCTCCAATTTACCATACGCAGCACCTGAATTA 17 0.42500000000000004 No Hit GGCAAACATGACCATTACACAACGTAGTATTATTTTTACAGAAACTAGTG 17 0.42500000000000004 No Hit GTATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCTATAAACAT 16 0.4 No Hit GCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGC 16 0.4 No Hit GTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCAT 15 0.375 No Hit GTTACAGACGCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCA 15 0.375 No Hit TTTTTTTTTTAGAATAGCAAGTTCACAGATCAGATTAAACCATGCAAGTT 15 0.375 No Hit CTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGCA 15 0.375 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 15 0.375 No Hit GCTTGATACAATGCAATGCCATCACGTGATATTCAGCTGCAGGGGCACAA 15 0.375 No Hit CTGCAACTAAGATCCTGATTCCTGAGTACGTGTATACACATATTTTTGGT 14 0.35000000000000003 No Hit ATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCTATAAACATTCATC 14 0.35000000000000003 No Hit GTCGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCC 14 0.35000000000000003 No Hit CCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATAC 14 0.35000000000000003 No Hit GCACCATTGTGAAACGAGAGGCCAATGGCCGACGATATAGAGGTCCTTGG 13 0.325 No Hit ATCCAAGCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGG 13 0.325 No Hit TTTTTTTTTAGAATAGCAAGTTCACAGATCAGATTAAACCATGCAAGTTT 13 0.325 No Hit CCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAA 13 0.325 No Hit CAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCTA 12 0.3 No Hit ACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAG 11 0.27499999999999997 No Hit GTAGGAAGTTCTCCAACTAACCCGATTCCACTCGCTAAAAAGATTTGCAG 11 0.27499999999999997 No Hit AGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAGCACT 11 0.27499999999999997 No Hit CTCCAATTTACCATACGCAGCACCTGAATTACAGGGGATTCAGCACCAAA 11 0.27499999999999997 No Hit CCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGA 11 0.27499999999999997 No Hit CTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAA 11 0.27499999999999997 No Hit CGTAGTATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCTATAA 11 0.27499999999999997 No Hit GCGCGTGCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAA 11 0.27499999999999997 No Hit GTACCACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTA 11 0.27499999999999997 No Hit CTGGCTTAGGATTTTTCTCTCTGTAAGTATGCTTGAGTTCTGAAAGCCTC 10 0.25 No Hit CTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACA 10 0.25 No Hit AGGAGGAATAGTATCGGGAACTGATGAGAGTTCCACTTTAAAATGGCTAG 10 0.25 No Hit AGAACAAAAACCTGCTGCTCCAATTTACCATACGCAGCACCTGAATTACA 10 0.25 No Hit GAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTAC 10 0.25 No Hit GTTAGTACCACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTA 10 0.25 No Hit CAAGCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTA 10 0.25 No Hit GTCTTCAAGGACAATCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACC 10 0.25 No Hit GGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAA 10 0.25 No Hit CCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGG 10 0.25 No Hit CTTGGATATGGAGGAACTGTTAATCATGATTAGATTTTTTTTCAAAAAGG 10 0.25 No Hit GTCCGGTATCTCCTCTTACTCCACATTTCACCAAAAGTGATTTCTCTGTA 10 0.25 No Hit AAGCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTAC 9 0.22499999999999998 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 9 0.22499999999999998 No Hit GCATCCTTGGCATCAATCGTTTCTGAGACATCATGAGTTTTTCCCTTCCC 9 0.22499999999999998 No Hit GCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATAC 9 0.22499999999999998 No Hit ATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCT 9 0.22499999999999998 No Hit TTTTTTTTTCAGCAATGAAACCAACTTTGTTTGATCAAACAGATTTCAGA 9 0.22499999999999998 No Hit ATTTTTACTGCTGAGATGGCGACAGCAAGAGTAGTACTCCGTATAAGGTA 9 0.22499999999999998 No Hit TGGCAAACATGACCATTACACAACGTAGTATTATTTTTACAGAAACTAGT 9 0.22499999999999998 No Hit TGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTG 9 0.22499999999999998 No Hit ATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCTATAAACATTC 9 0.22499999999999998 No Hit CAACAAGATTGATTGTGCCATCACTGATGGCTGTGTAAATCTTAACACTC 9 0.22499999999999998 No Hit GTGAGGGCCTAGCTGGAGCAGATGGAGCATTAATTGGTTGGGCCCTGTGT 9 0.22499999999999998 No Hit GGTTAGTACCACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTT 9 0.22499999999999998 No Hit GCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTG 9 0.22499999999999998 No Hit CTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAAAAAGAGAAA 9 0.22499999999999998 No Hit GCCATCACGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGC 8 0.2 No Hit GCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAG 8 0.2 No Hit CCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTGGCGCGAT 8 0.2 No Hit GGTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCA 8 0.2 No Hit TCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCC 8 0.2 No Hit GGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTATCCGGTGAT 8 0.2 No Hit GACCACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAG 8 0.2 No Hit GTCCTTGGCAGCACAAGCAAAAGCAGTAAAAAAAGACAGGGCGTCGAAAT 8 0.2 No Hit GTATTTTCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTT 8 0.2 No Hit CTCTACTAGATCTGCACACAGGGAAGTGCAAACTCTACAGGTTTTTGGTC 8 0.2 No Hit GTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACAAC 8 0.2 No Hit ACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAAC 8 0.2 No Hit CTGGCAAACATGACCATTACACAACGTAGTATTATTTTTACAGAAACTAG 8 0.2 No Hit CACTTCTATGCAACTGGCAAACATGACCATTACACAACGTAGTATTATTT 8 0.2 No Hit CTGCGCGTTGCCGCCGCCCATGGCTTCTTTCTCCTCGGCGAGAACGAGAG 8 0.2 No Hit CATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAG 8 0.2 No Hit GGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATA 8 0.2 No Hit GGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTC 8 0.2 No Hit CCAATGCTTGCTCGTCTCCTCCTTGCAGTGGGCCCGCCCATTGAACGAGC 8 0.2 No Hit GTGGTAGCTCTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAA 8 0.2 No Hit GCAGTTTACAATCTTCAGGTTCTGTAGTTCAGGAAACAAAGTTCTCAGGT 8 0.2 No Hit CACGAGTTCAGGGCAATCTGTAATTTCGACAGCCTCTAGACAAGAGAAAA 8 0.2 No Hit AACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAACACTGC 7 0.17500000000000002 No Hit TTTTTTTTTTTACTCCCGATGCATCGTGAACTTCAGCAACATTTGTCCAA 7 0.17500000000000002 No Hit CACTTCTGGAAGATCTGAAAGAATGTCTTCAAGGACAATCGGCTTCGCTA 7 0.17500000000000002 No Hit ACCACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGC 7 0.17500000000000002 No Hit CGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCT 7 0.17500000000000002 No Hit GCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTGGCGCGATGACAACT 7 0.17500000000000002 No Hit GGCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGC 7 0.17500000000000002 No Hit CACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTACAAG 7 0.17500000000000002 No Hit CTACTAGATCTGCACACAGGGAAGTGCAAACTCTACAGGTTTTTGGTCTA 7 0.17500000000000002 No Hit CAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCA 7 0.17500000000000002 No Hit CTTCTATGCAACTGGCAAACATGACCATTACACAACGTAGTATTATTTTT 7 0.17500000000000002 No Hit GGATCTATGAGCTTCTGAACAATTGGATGTTCTGCAAGTAGCAGAACTTG 7 0.17500000000000002 No Hit CAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAGC 7 0.17500000000000002 No Hit GCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCT 7 0.17500000000000002 No Hit GCCCGAAACTTGTCTGGACTATTAACGCTTCAATGGAAATTATAGCCATG 7 0.17500000000000002 No Hit AGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAGCA 7 0.17500000000000002 No Hit TATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCTATAAACATT 7 0.17500000000000002 No Hit GGTAGCTCTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAAAA 6 0.15 No Hit TTTTTTTTTACGATGCCACCATTAGGGCAAGAAATATTGATTATCAAGTA 6 0.15 No Hit GTTGGTGCTAATGGATGAGAAAAGCAGTTTCTTAAGGAAATGGCATTCTT 6 0.15 No Hit AGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACA 6 0.15 No Hit ATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCC 6 0.15 No Hit GGAGGAACTGTTAATCATGATTAGATTTTTTTTCAAAAAGGAGGGAAGCC 6 0.15 No Hit GTCTCACAACATCAAGGTTTAGGCAACAGACCAAAACTGCAACCAGTTAA 6 0.15 No Hit TTTTTTTTTACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAAC 6 0.15 No Hit GCCATTACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAAT 6 0.15 No Hit GCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAGCACTGCCCCTTCCCTG 6 0.15 No Hit GCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAG 6 0.15 No Hit GCTAGGGTAAATGCATTGCACCATTGTGAAACGAGAGGCCAATGGCCGAC 6 0.15 No Hit GGTGGAAACATCTTGTAATCTTTTTGCATAAAAAAGCCCTGGCATTCCTC 6 0.15 No Hit CGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCAT 6 0.15 No Hit AGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGC 6 0.15 No Hit CATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGA 6 0.15 No Hit CAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATGATGCACCT 6 0.15 No Hit CAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTGGCGCGATGAC 6 0.15 No Hit AAGCAATCAGCACACCGAAGATCCACTGCAACTAAGATCCTGATTCCTGA 6 0.15 No Hit GTACCGTACAGTGACCAGTGAACCCGACCCATGTGCACCGTGATAAAGTC 6 0.15 No Hit GGCCATTACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAA 6 0.15 No Hit GTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGC 6 0.15 No Hit TTACAGTATTTTCAGGTTCTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTC 6 0.15 No Hit GCTCGTCTCCTCCTTGCAGTGGGCCCGCCCATTGAACGAGCTTTTTTATT 6 0.15 No Hit GTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAG 6 0.15 No Hit CTCGTCTCCTCCTTGCAGTGGGCCCGCCCATTGAACGAGCTTTTTTATTC 6 0.15 No Hit GTAAAAATCGACAGCTAAAGAAACCAATTGAAGGACATTCTTTGCTTGAT 6 0.15 No Hit GCCAAACAAATAGGTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGG 6 0.15 No Hit GCAACCTGGTCCGGTATCTCCTCTTACTCCACATTTCACCAAAAGTGATT 6 0.15 No Hit GAGCACATGAGGATGCAGATCCTGTTAACCATGGATCATCTCCTGAATCA 6 0.15 No Hit TTTTTTTTTGAAAGGCGAAGGCATCACCAAGATATACTATTTCTGTCAGT 6 0.15 No Hit CTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACAACTAT 6 0.15 No Hit CTGGAAGATCTGAAAGAATGTCTTCAAGGACAATCGGCTTCGCTAGTTGC 6 0.15 No Hit GCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTT 5 0.125 No Hit CGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAA 5 0.125 No Hit GCCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCG 5 0.125 No Hit GTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAG 5 0.125 No Hit CCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCC 5 0.125 No Hit GCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTC 5 0.125 No Hit CTCAAAGAACTTGTCAACAAGATTGATTGTGCCATCACTGATGGCTGTGT 5 0.125 No Hit GTGACCATTACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAAC 5 0.125 No Hit GTACCATCTTTTTTAATCTTTTTCCTACCAAAGATTGTTTTCATTGATCT 5 0.125 No Hit GGGAAGTGCAAACTCTACAGGTTTTTGGTCTATTAAAGTACACATATACA 5 0.125 No Hit GGCAGGCGAACAGCGACCAGGTAGCAAAGCCAACCAACAGGGACTGAGGG 5 0.125 No Hit CCCGGACAACCACCACTGCCCTTTCTTCTCAGGGTCCAAAAGCTTACTCC 5 0.125 No Hit CTAGCGAATTACTTGCATCCTTGGCATCAATCGTTTCTGAGACATCATGA 5 0.125 No Hit CCTGACTCCAAAAGTTTCATCAAGCAATCAGCACACCGAAGATCCACTGC 5 0.125 No Hit CAGGGAAGTGCAAACTCTACAGGTTTTTGGTCTATTAAAGTACACATATA 5 0.125 No Hit GGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAAATAC 5 0.125 No Hit ACTTAAACCACAACTATCAATATACCTGGAATAAAAAAAAGAGAAATGGT 5 0.125 No Hit TCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGT 5 0.125 No Hit CTACAGGTTTTTGGTCTATTAAAGTACACATATACATCATAACTCAGCTT 5 0.125 No Hit CTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACC 5 0.125 No Hit GCCGGCCTCTCCATCTCTACCTCCGCGGGGGGAAAAGGCCTGCCGTTCGT 5 0.125 No Hit CTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCCT 5 0.125 No Hit CAGGTTTTTGGTCTATTAAAGTACACATATACATCATAACTCAGCTTCCC 5 0.125 No Hit ACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAATGGAAGTCT 5 0.125 No Hit GTGAACCATTTGTTATAAAAGTATTGATGAACCTTTTTTATACTGTTCAA 5 0.125 No Hit GAGGAATAGTATCGGGAACTGATGAGAGTTCCACTTTAAAATGGCTAGGA 5 0.125 No Hit GCAGCACCTGAATTACAGGGGATTCAGCACCAAAAACCTACTAGGGTAAC 5 0.125 No Hit ACAGGTTTTTGGTCTATTAAAGTACACATATACATCATAACTCAGCTTCC 5 0.125 No Hit GCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATT 5 0.125 No Hit GGATTGACTGAACCCAGATGAAAAAGAAATTACTACATGTATTCTAGGTG 5 0.125 No Hit CGCAAAAGCATGTTAAATAGCAAGGCACATTGCCAAAAAAAAATAGAACA 5 0.125 No Hit GGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGT 5 0.125 No Hit CTTGACGGCAGCCACACTTCATGGGATAGGGAATTTTTCTATCATGACGT 5 0.125 No Hit GGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 5 0.125 No Hit TTTTTTTTTTTTAGAATAGCAAGTTCACAGATCAGATTAAACCATGCAAG 5 0.125 No Hit TACACAACGTAGTATTATTTTTACAGAAACTAGTGATGCAACAATGGAAG 5 0.125 No Hit CCATCACGTGATATTCAGCTGCAGGGGCACAAGGAAAAAAACATCCGGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTTTT 330 3.0389856E-8 21.505682 1 >>END_MODULE ERR5262806 read2 length is 50-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262806_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 50-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0185 37.0 37.0 37.0 37.0 37.0 2 35.709 37.0 37.0 37.0 37.0 37.0 3 35.812 37.0 37.0 37.0 37.0 37.0 4 35.9265 37.0 37.0 37.0 37.0 37.0 5 35.921 37.0 37.0 37.0 37.0 37.0 6 35.951 37.0 37.0 37.0 37.0 37.0 7 35.922 37.0 37.0 37.0 37.0 37.0 8 36.0215 37.0 37.0 37.0 37.0 37.0 9 35.96 37.0 37.0 37.0 37.0 37.0 10-14 36.0386 37.0 37.0 37.0 37.0 37.0 15-19 36.029399999999995 37.0 37.0 37.0 37.0 37.0 20-24 35.9288 37.0 37.0 37.0 37.0 37.0 25-29 35.99050000000001 37.0 37.0 37.0 37.0 37.0 30-34 35.89790000000001 37.0 37.0 37.0 37.0 37.0 35-39 35.8858 37.0 37.0 37.0 37.0 37.0 40-44 35.856399999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.9127 37.0 37.0 37.0 37.0 37.0 50-54 35.79825493873468 37.0 37.0 37.0 37.0 37.0 55-59 35.738784696174044 37.0 37.0 37.0 37.0 37.0 60-64 35.6655663915979 37.0 37.0 37.0 37.0 37.0 65-69 35.74228557139285 37.0 37.0 37.0 37.0 37.0 70-74 35.67103601019094 37.0 37.0 37.0 37.0 37.0 75-79 35.63039278958719 37.0 37.0 37.0 37.0 37.0 80-84 35.54001310446868 37.0 37.0 37.0 37.0 37.0 85-89 35.589944879129206 37.0 37.0 37.0 37.0 37.0 90-94 35.521794415899464 37.0 37.0 37.0 37.0 37.0 95-99 35.45068223040438 37.0 37.0 37.0 37.0 37.0 100-104 35.46013326619252 37.0 37.0 37.0 34.6 37.0 105-109 35.324973329613655 37.0 37.0 37.0 32.2 37.0 110-114 35.32812332570258 37.0 37.0 37.0 32.2 37.0 115-119 35.365248108176004 37.0 37.0 37.0 37.0 37.0 120-124 35.26881266913119 37.0 37.0 37.0 32.2 37.0 125-129 35.220170403651764 37.0 37.0 37.0 27.4 37.0 130-134 35.15569060299743 37.0 37.0 37.0 27.4 37.0 135-139 35.11820150877061 37.0 37.0 37.0 27.4 37.0 140-144 35.09422077053214 37.0 37.0 37.0 25.0 37.0 145-149 35.02584352793237 37.0 37.0 37.0 25.0 37.0 150 35.11278195488722 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 1.0 13 1.0 14 0.0 15 0.0 16 2.0 17 0.0 18 2.0 19 5.0 20 2.0 21 7.0 22 8.0 23 8.0 24 11.0 25 10.0 26 6.0 27 17.0 28 26.0 29 32.0 30 37.0 31 61.0 32 85.0 33 122.0 34 232.0 35 677.0 36 2447.0 37 199.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.824999999999996 22.15 9.225 24.8 2 26.0 25.724999999999998 28.125 20.150000000000002 3 19.2 25.05 34.449999999999996 21.3 4 23.625 30.95 21.925 23.5 5 24.8 34.849999999999994 19.900000000000002 20.45 6 22.75 34.35 23.45 19.45 7 20.0 19.950000000000003 39.475 20.575 8 19.975 22.575 28.599999999999998 28.849999999999998 9 20.925 23.325000000000003 29.7 26.05 10-14 23.200000000000003 26.174999999999997 27.325 23.3 15-19 23.885 25.080000000000002 28.035 23.0 20-24 23.185 25.419999999999998 28.875 22.52 25-29 23.405 24.915000000000003 29.080000000000002 22.6 30-34 23.805 24.845 28.884999999999998 22.465 35-39 23.04 25.415 28.694999999999997 22.85 40-44 23.185 26.88 28.585 21.349999999999998 45-49 23.09 24.805 29.404999999999998 22.7 50-54 22.619523904780955 26.280256051210245 28.690738147629524 22.409481896379276 55-59 22.09552388097024 25.841460365091272 29.492373093273315 22.570642660665165 60-64 21.885471367841962 25.971492873218306 28.97224306076519 23.170792698174544 65-69 23.415853963490875 26.401600400100023 28.562140535133786 21.62040510127532 70-74 23.808332916520783 25.443905366878404 28.564997749212225 22.182763967388585 75-79 23.056904058855913 25.894599869876384 28.67223862669536 22.376257444572346 80-84 23.52705611453171 26.840866997046604 28.092306152074887 21.5397707363468 85-89 23.198638025136447 26.4483501076561 28.606479395122932 21.746532472084525 90-94 23.87491229828606 25.308208880424978 29.342487721760047 21.474391099528916 95-99 24.102486963497793 25.105294825511432 29.778379462494986 21.01383874849579 100-104 24.829282988551917 26.169913637276558 27.18417352882105 21.816629845350473 105-109 24.740763112856136 25.9589247961341 28.209000302023558 21.091311788986207 110-114 23.308574604618258 26.10782678995503 28.86665656106311 21.7169420443636 115-119 23.297288986829294 26.66427509865218 28.71931532824271 21.319120586275815 120-124 23.623068146457836 25.702727932787056 29.410849496940152 21.263354423814956 125-129 23.873755203119238 25.565098266505082 29.07423994941778 21.4869065809579 130-134 24.074768357345615 25.708317711959722 28.766536339778266 21.450377590916396 135-139 23.744342040682774 25.145879914926105 29.110541528058025 21.9992365163331 140-144 23.054143152483654 24.72481421467285 30.172654634855007 22.04838799798849 145-149 23.722021867307802 25.055813154731236 29.400652584578395 21.821512393382562 150 25.910931174089068 24.493927125506072 29.207634470792364 20.387507229612492 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.5 18 0.5 19 0.0 20 1.0 21 1.0 22 0.5 23 0.5 24 1.5 25 3.5 26 3.5 27 6.0 28 8.5 29 22.5 30 38.5 31 48.0 32 62.0 33 73.0 34 79.5 35 94.5 36 107.0 37 113.5 38 135.5 39 168.0 40 175.5 41 195.0 42 218.5 43 190.5 44 189.0 45 193.5 46 172.5 47 148.5 48 143.0 49 141.5 50 100.0 51 122.5 52 157.5 53 98.5 54 82.0 55 80.0 56 59.0 57 61.5 58 66.5 59 59.5 60 39.5 61 36.0 62 40.5 63 36.5 64 25.5 65 21.5 66 15.5 67 19.5 68 25.5 69 26.5 70 18.5 71 13.5 72 14.0 73 11.0 74 10.5 75 5.5 76 4.0 77 4.0 78 2.5 79 1.5 80 0.0 81 1.0 82 1.0 83 1.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 50-54 1.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 2.0 75-79 1.0 80-84 1.0 85-89 3.0 90-94 2.0 95-99 4.0 100-104 8.0 105-109 9.0 110-114 45.0 115-119 54.0 120-124 45.0 125-129 68.0 130-134 58.0 135-139 84.0 140-144 84.0 145-149 73.0 150-151 3458.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.825 #Duplication Level Percentage of deduplicated Percentage of total 1 59.24764890282132 33.074999999999996 2 22.480967308553517 25.1 3 9.493954321540528 15.9 4 4.343931930138827 9.700000000000001 5 1.9256605463502015 5.375 6 1.3434841021047919 4.5 7 0.40304523063143755 1.575 8 0.22391401701746527 1.0 9 0.08956560680698611 0.44999999999999996 >10 0.44782803403493054 3.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCACTCCACTCTCTCTCTCTCTGTTCGCCTCTCGCCTTTTTAGAGAGA 19 0.475 No Hit GCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCC 18 0.44999999999999996 No Hit GAAAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCC 14 0.35000000000000003 No Hit GTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGA 14 0.35000000000000003 No Hit CTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTTC 13 0.325 No Hit GTTCATTTCACAAGAAGTCCGCAGTCCACAACAATATCCTCATAGCTAAA 13 0.325 No Hit AAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCCTC 11 0.27499999999999997 No Hit AGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACC 11 0.27499999999999997 No Hit CCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTT 10 0.25 No Hit GTTTGGTTTTCAGAGTCCTATCTTCGGATATCGAACCTCTTTTTAATAAT 10 0.25 No Hit GATGGGTTAAAATTGCCGGAAAATGGTGTTCATTTCACAAGAAGTCCGCA 9 0.22499999999999998 No Hit GGATGATGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTT 9 0.22499999999999998 No Hit ATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAA 8 0.2 No Hit GCTACTCTTGCTGAGGGCGCTTCTGAGAGCCTTCACGTCAAGGACTACAA 8 0.2 No Hit GTCCAAAACCTCGGCAGTCTTCATCCCTCACGGGCCAGGTGCTGTCAAAG 8 0.2 No Hit ATTAGATTCTCTGTGTGATGATGGACATCTGGACTGCAATAATATGCTGT 8 0.2 No Hit GTGGAGTCAGATCCTCCGAGGACTCGGGAACTTGATACTGTTGGTGTGCT 8 0.2 No Hit AACCAAATATTGTGTAGCATGTTTTATCCTGTTACTGTATCTCTTTTTTA 7 0.17500000000000002 No Hit TGGTATTTATCCTGCTGTCGATCCTTTGGACTCCACATCCAGAATGTTGT 7 0.17500000000000002 No Hit GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 7 0.17500000000000002 No Hit GCTGAAGTATGATGTGGAGTCAGATCCTCCGAGGACTCGGGAACTTGATA 7 0.17500000000000002 No Hit GTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGA 7 0.17500000000000002 No Hit ATTCCATTGGTCTGCTTTCATAATGATCGTGCAAAGTTTGTGGCTGGGTA 7 0.17500000000000002 No Hit AGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTT 7 0.17500000000000002 No Hit GGAAAATTGTTGCTTCAATGCATCACGGATTGACTATTACTTGGAGTATG 7 0.17500000000000002 No Hit AATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGT 7 0.17500000000000002 No Hit TGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTTTATGGG 6 0.15 No Hit ATTTTTTTATGGGAATTGGACAGAATCCTGTGTGTAGTTAAGTGAGCGTA 6 0.15 No Hit GGTGTATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACAT 6 0.15 No Hit GGAATATCGCAATTTATATTTATTTTTTTGAGGAGACGCAATTTTTATAT 6 0.15 No Hit GAAAACTTGACCCAACTTGAGCAACTGTTATGTCTTGGTTATGCTTGACC 6 0.15 No Hit GGACATGGTCTTGGTGACCCAATATTTTGACACTATGAAGGATATCGGGG 6 0.15 No Hit GAATGGATGAGCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGG 6 0.15 No Hit GGTTGAAGATGTCTATCCATCATCAGAGCTGGAATGTGTACTGAACAACG 6 0.15 No Hit GTCCACAACAATATCCTCATAGCTAAATATTTTGTGAGATGAAACTTAAG 6 0.15 No Hit GAAACTTAAGGAAACAATACTATTATTTTGTGAGTGGCACAGTATATCTT 6 0.15 No Hit GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 6 0.15 No Hit GTGCAAAATTACTTAACTGGATCTTTACCAGCATTCATTGGAAAGCTGAC 6 0.15 No Hit CTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTT 6 0.15 No Hit CCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTT 6 0.15 No Hit GTCCTATCTTCGGATATCGAACCTCTTTTTAATAATATGCAACTTGCAAC 6 0.15 No Hit GTCAGATCCTCCGAGGACTCGGGAACTTGATACTGTTGGTGTGCTTGACC 6 0.15 No Hit CCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTC 6 0.15 No Hit AGAAGATGTACTTGCCTCCCGGCGATCATCCGGTTGAAGATGTCTATCCA 6 0.15 No Hit CATCGCACAACTCTCTTAGCCGCCTCCGTCCTTGCCCTAGCCCCCCGGCG 6 0.15 No Hit GCAGTCCACAACAATATCCTCATAGCTAAATATTTTGTGAGATGAAACTT 6 0.15 No Hit AGACGGGAGCAGCGCTTCTTCCTGTGGTTCGATCCCACGGCGGATTTCCA 6 0.15 No Hit CTCTGTGTGATGATGGACATCTGGACTGCAATAATATGCTGTCTTCGATA 6 0.15 No Hit CTCGTACATGCCCAGCAGCGGCCACCCTTATGGTCAGCTCCATGCCCGCA 6 0.15 No Hit TGTTGCTTCAATGCATCACGGATTGACTATTACTTGGAGTATGAACCTCA 6 0.15 No Hit AGGATATCGGGGCCACGTCCAAAACCTCGGCAGTCTTCATCCCTCACGGG 6 0.15 No Hit AGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTC 6 0.15 No Hit CGTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTACA 6 0.15 No Hit GGATTGACTATTACTTGGAGTATGAACCTCATCCATCATCAACTAAAAAC 6 0.15 No Hit CAGGCCTCTTCCAATCCTTTTGCCGGCTCGTACATGCCCAGCAGCGGCCA 6 0.15 No Hit GCAATCAACCAGTAGCGCTGCCCTCAACCTCAATTTTGGATCAGCTTTTA 6 0.15 No Hit GCTGCTTGTCTTTACATTGCCTCCGGCAAAGTAAAAAAGCTTATCTTCTT 5 0.125 No Hit GTCCATGGCGGAGGTCCTTGAGGTGGCCCTCCGGTGCACGAGGCCGGCGC 5 0.125 No Hit CCCGCCCCAAGCCCGGCGTCGAGCGCCTCGCCGTCTCCCTCGACCTCTAC 5 0.125 No Hit GTTCGACTCCACTCCACTCTCTCTCTCTCTGTTCGCCTCTCGCCTTTTTA 5 0.125 No Hit AAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTG 5 0.125 No Hit ATGAATCAGAACATGGACTCAATGGACAGTATGATTGGAAGGATATTCAA 5 0.125 No Hit ATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTG 5 0.125 No Hit AAATATGCGTTCATATTAGTGTGTGTGCACCATCATCTTTTGAAATATTA 5 0.125 No Hit TAGGGATCATGGCTGCTGGATGTTATGGCGTTCCACAATTTCGGATGCGT 5 0.125 No Hit GCCACGTCCAAAACCTCGGCAGTCTTCATCCCTCACGGGCCAGGTGCTGT 5 0.125 No Hit GAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGC 5 0.125 No Hit CAGCCTTTTCATGTTGCTGAAGTGTTCACAGGTGCACCTGGAAAGTATGT 5 0.125 No Hit CATCAGGCCTCTTCCAATCCTTTTGCCGGCTCGTACATGCCCAGCAGCGG 5 0.125 No Hit GTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATG 5 0.125 No Hit GTCTGGCCTTCTGCCTATGAGTATTGTTTGGTTTTCAGAGTCCTATCTTC 5 0.125 No Hit ACAAGAAGTCCGCAGTCCACAACAATATCCTCATAGCTAAATATTTTGTG 5 0.125 No Hit GCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGC 5 0.125 No Hit CAAAAGTCCTCCCAAGAGGTTTCTAATCATATATGGTTACATGGTACAAC 5 0.125 No Hit GTGAGATGAAACTTAAGGAAACAATACTATTATTTTGTGAGTGGCACAGT 5 0.125 No Hit GAAGACATTAATTGTCATACACCGTGCCCTTCGAGAAGTTGATCCCACAT 5 0.125 No Hit GCTGACTTCATTCTGACTTACACTGATGAGGACGGGGATGTTGTCATGCT 5 0.125 No Hit GTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCC 5 0.125 No Hit AGTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAG 5 0.125 No Hit GCAGCAGTACTACCAGCAGATGATGATGATGACTGGACAACAGTCTGTTC 5 0.125 No Hit GATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGGT 5 0.125 No Hit ACTGCAGCAACTTCTTTTTCGGCTGCTTGCTTGCCAGCCACAAGGTGCAT 5 0.125 No Hit AAGAAGTCCTGTTCCAGATCATAAACTACGCGAATGAGAAAAAAGATCAC 5 0.125 No Hit CTTGAATGTTTCCGAGTGCTGAAGTATGATGTGGAGTCAGATCCTCCGAG 5 0.125 No Hit CTTAAGGAAACAATACTATTATTTTGTGAGTGGCACAGTATATCTTATTC 5 0.125 No Hit CATTCACAATGCCTGAAATTGCAAAGATGATTAGTTCTGCTGCACTTCTT 5 0.125 No Hit CATGCTTTGGTTAAATTATGTATGAGTAAGGATCTGTTTGGTTTCAGCCC 5 0.125 No Hit CTCCAGGCCAAGATGGGTTAAAATTGCCGGAAAATGGTGTTCATTTCACA 5 0.125 No Hit GGCCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATG 5 0.125 No Hit GTTAAAATTGCCGGAAAATGGTGTTCATTTCACAAGAAGTCCGCAGTCCA 5 0.125 No Hit CCGCAGTCCACAACAATATCCTCATAGCTAAATATTTTGTGAGATGAAAC 5 0.125 No Hit CTTGACCCAACTTGAGCAACTGTTATGTCTTGGTTATGCTTGACCTACCT 5 0.125 No Hit GTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTACAT 5 0.125 No Hit CAACAATATCCTCATAGCTAAATATTTTGTGAGATGAAACTTAAGGAAAC 5 0.125 No Hit GTTGCTGAAGTGTTCACAGGTGCACCTGGAAAGTATGTTGAACTAAAGGA 5 0.125 No Hit TATATCGCTCACGACCTGTTGCACAAGGAAACGCCTCCACTGGACTTCTG 5 0.125 No Hit CATGGAAGGTGCTGAGCGACGACGTGGCAGGCGCCCCGCACTGCCTCGCC 5 0.125 No Hit GTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGA 5 0.125 No Hit GATCAGCAAGCCGCCCCAGTGGCATCATCAGCTCCGATGGCAATGACGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.025 0.0 0.0 0.0 0.0 122-123 0.025 0.0 0.0 0.0 0.0 124-125 0.025 0.0 0.0 0.0 0.0 126-127 0.025 0.0 0.0 0.0 0.0 128-129 0.025 0.0 0.0 0.0 0.0 130-131 0.025 0.0 0.0 0.0 0.0 132-133 0.025 0.0 0.0 0.0 0.0 134-135 0.025 0.0 0.0 0.0 0.0 136-137 0.025 0.0 0.0 0.0 0.0 138 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATGATT 10 0.007271448 142.0 6 AGATTCT 10 0.007271448 142.0 4 >>END_MODULE Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756133 spots for ERR5262806.sra Written 1756133 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra Read 1756124 spots for ERR5262806.sra Written 1756124 spots for ERR5262806.sra SRR ids: ['ERR5262806.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ahdcumea ERR5262806.sra spots: 35122489 blocks: [[1, 1756124], [1756125, 3512248], [3512249, 5268372], [5268373, 7024496], [7024497, 8780620], [8780621, 10536744], [10536745, 12292868], [12292869, 14048992], [14048993, 15805116], [15805117, 17561240], [17561241, 19317364], [19317365, 21073488], [21073489, 22829612], [22829613, 24585736], [24585737, 26341860], [26341861, 28097984], [28097985, 29854108], [29854109, 31610232], [31610233, 33366356], [33366357, 35122489]] ERR5262806 file size 11469965 ERR5262806 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262806 ERR5262806_1.fastq ERR5262806_2.fastq Input file: ERR5262806_1.fastq Paired file: ERR5262806_2.fastq trimmed: ERR5262806-trimmed-pair1.fastq, ERR5262806-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:20:28 2024 >> started Fri Dec 6 12:23:19 2024 >> done (171.278s) 35122489 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 35122489 (100.00%) read pairs available; of these: 10612 ( 0.03%) trimmed read pairs available after processing 35111877 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 2 0.00% 25 2 0.00% 26 2 0.00% 27 1 0.00% 28 0 0.00% 29 1 0.00% 30 4 0.00% 31 0 0.00% 32 1 0.00% 33 3 0.00% 34 2 0.00% 35 5 0.00% 36 4 0.00% 37 2 0.00% 38 2 0.00% 39 2 0.00% 40 0 0.00% 41 0 0.00% 42 1 0.00% 43 0 0.00% 44 0 0.00% 45 2 0.00% 46 1 0.00% 47 2 0.00% 48 4 0.00% 49 505 0.00% 50 586 0.00% 51 670 0.00% 52 738 0.00% 53 747 0.00% 54 845 0.00% 55 917 0.00% 56 1118 0.00% 57 1248 0.00% 58 1323 0.00% 59 1694 0.00% 60 1953 0.01% 61 2245 0.01% 62 2491 0.01% 63 2716 0.01% 64 2888 0.01% 65 3184 0.01% 66 3585 0.01% 67 3846 0.01% 68 4630 0.01% 69 5534 0.02% 70 6374 0.02% 71 6958 0.02% 72 7983 0.02% 73 8791 0.03% 74 9483 0.03% 75 10556 0.03% 76 11198 0.03% 77 12136 0.03% 78 13732 0.04% 79 15213 0.04% 80 16799 0.05% 81 19348 0.06% 82 21490 0.06% 83 23448 0.07% 84 25880 0.07% 85 27357 0.08% 86 28589 0.08% 87 30590 0.09% 88 32092 0.09% 89 33787 0.10% 90 36991 0.11% 91 39769 0.11% 92 42590 0.12% 93 46433 0.13% 94 48948 0.14% 95 50848 0.14% 96 52945 0.15% 97 53910 0.15% 98 55513 0.16% 99 57309 0.16% 100 59237 0.17% 101 62296 0.18% 102 65618 0.19% 103 70008 0.20% 104 72031 0.21% 105 75685 0.22% 106 76739 0.22% 107 77079 0.22% 108 79539 0.23% 109 80064 0.23% 110 81727 0.23% 111 84666 0.24% 112 87733 0.25% 113 89298 0.25% 114 94907 0.27% 115 96926 0.28% 116 97352 0.28% 117 98324 0.28% 118 98694 0.28% 119 99144 0.28% 120 101146 0.29% 121 102738 0.29% 122 103401 0.29% 123 107437 0.31% 124 111155 0.32% 125 112264 0.32% 126 114702 0.33% 127 114439 0.33% 128 114163 0.33% 129 116569 0.33% 130 116041 0.33% 131 116035 0.33% 132 118385 0.34% 133 119864 0.34% 134 122469 0.35% 135 125331 0.36% 136 125970 0.36% 137 126395 0.36% 138 127755 0.36% 139 129530 0.37% 140 127910 0.36% 141 131530 0.37% 142 133215 0.38% 143 135059 0.38% 144 135945 0.39% 145 136950 0.39% 146 142989 0.41% 147 306608 0.87% 148 130068 0.37% 149 128325 0.37% 150 28750466 81.86% 35122489 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=2.41 fanout-score-rank=41 prefix-density=0.19 prefix-fanout=2.3 sequence=CCCTTGAAACCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=19 fanout-score=220.89 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=9.7 sequence=CCTTCTCCAGTCCAGCTCTTTCAAACTCGTTCAGTGGACCAAGTGGCAAGATCTCCTCAACGCCACATCGACCTAGGCGCACTTTGGATGCAAAGAAAGGCAGCTCCGTCACCTGAGAAGCTACATAAGAGCACTCTACTATCCCAGCATCACCATGCAATCCTCTTAAGCAAGCATCTGCGAATTTTGCTGCTGCATACGCCATAGAAAGAGTTGCTGATCCT criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.48 fanout-score-rank=38 prefix-density=0.28 prefix-fanout=2.3 sequence=ACCTTGTCAAGA criterion=fanout-score sequence-density=0.13 sequence-density-rank=7 fanout-score=103.26 fanout-score-rank=1 prefix-density=0.78 prefix-fanout=17.1 sequence=CGCCGCCGCCGC ERR5262806 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:23:45 Started mapping on | Dec 06 12:23:45 Finished on | Dec 06 12:26:05 Mapping speed, Million of reads per hour | 903.15 Number of input reads | 35122489 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 33626251 Uniquely mapped reads % | 95.74% Average mapped length | 288.83 Number of splices: Total | 30227363 Number of splices: Annotated (sjdb) | 27939014 Number of splices: GT/AG | 29740657 Number of splices: GC/AG | 400977 Number of splices: AT/AC | 17602 Number of splices: Non-canonical | 68127 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.01% Deletion average length | 2.28 Insertion rate per base | 0.01% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 357973 % of reads mapped to multiple loci | 1.02% Number of reads mapped to too many loci | 1050 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.23% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1138265 1138265 1138265 N_multimapping 357973 357973 357973 N_noFeature 1639738 32588646 2028167 N_ambiguous 765978 4702 116911 UnstrandedReadsAssigned:31220535 PositiveStrandReadsAssigned:1032903 NegativeStrandReadsAssigned:31481173 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262806 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262806-trimmed-pair1.fastq ERR5262806-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 35,122,489 reads, 31,870,653 reads pseudoaligned [quant] estimated average fragment length: 274.911 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,197 rounds 52973 ERR5262806.ke.tsv 35125 ERR5262806.se.tsv 88098 total ==> ERR5262806.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 663 0 0 PNS24247 1044 770.089 173.55 9.85157 PNS24249 1928 1654.09 495.633 13.0986 PNS24246 1044 770.089 173.55 9.85157 PNS24248 1044 770.089 173.55 9.85157 PNS24244 1471 1197.09 294.719 10.7623 PNS24243 293 100.419 2 0.87064 KQK14069 1603 1329.09 92508.8 3042.65 KQK14071 474 235.549 496.965 92.2289 ==> ERR5262806.se.tsv <== BRADI_1g14170v3 94170 BRADI_1g53295v3 285 BRADI_1g59795v3 1205 BRADI_1g07683v3 0 BRADI_1g00485v3 33 BRADI_1g20270v3 532 BRADI_1g74790v3 2719 BRADI_1g09890v3 0 BRADI_1g77505v3 321 BRADI_1g48960v3 0 ERR5262806 completed mapping pipeline successfully