Starting /dee2/code/volunteer_pipeline.sh ERR5262807 current disk space = 1551203622912 free memory = 1393471596 ERR5262807 SRAfilesize 1dac4ad19082eda52d81668c49e68cdf ERR5262807.sra ERR5262807.sra file validated ERR5262807 is paired end ERR5262807 is conventional basespace ERR5262807 read1 length is 71-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262807_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 71-150 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.58 37.0 37.0 37.0 37.0 37.0 2 36.31075 37.0 37.0 37.0 37.0 37.0 3 36.562 37.0 37.0 37.0 37.0 37.0 4 36.6655 37.0 37.0 37.0 37.0 37.0 5 36.672 37.0 37.0 37.0 37.0 37.0 6 36.7085 37.0 37.0 37.0 37.0 37.0 7 36.521 37.0 37.0 37.0 37.0 37.0 8 36.7085 37.0 37.0 37.0 37.0 37.0 9 36.6025 37.0 37.0 37.0 37.0 37.0 10-14 36.618100000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.5418 37.0 37.0 37.0 37.0 37.0 20-24 36.4774 37.0 37.0 37.0 37.0 37.0 25-29 36.44369999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.4726 37.0 37.0 37.0 37.0 37.0 35-39 36.37220000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.371500000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.2975 37.0 37.0 37.0 37.0 37.0 50-54 36.3509 37.0 37.0 37.0 37.0 37.0 55-59 36.3153 37.0 37.0 37.0 37.0 37.0 60-64 36.2781 37.0 37.0 37.0 37.0 37.0 65-69 36.2759 37.0 37.0 37.0 37.0 37.0 70-74 36.22928087021755 37.0 37.0 37.0 37.0 37.0 75-79 36.22080421969761 37.0 37.0 37.0 37.0 37.0 80-84 36.090049529330216 37.0 37.0 37.0 37.0 37.0 85-89 36.16879961645089 37.0 37.0 37.0 37.0 37.0 90-94 36.06675308274644 37.0 37.0 37.0 37.0 37.0 95-99 36.02716957418936 37.0 37.0 37.0 37.0 37.0 100-104 36.045989897034495 37.0 37.0 37.0 37.0 37.0 105-109 36.07187219569438 37.0 37.0 37.0 37.0 37.0 110-114 36.056765111810286 37.0 37.0 37.0 37.0 37.0 115-119 36.06242545441184 37.0 37.0 37.0 37.0 37.0 120-124 36.00138652865477 37.0 37.0 37.0 37.0 37.0 125-129 35.895111060858994 37.0 37.0 37.0 37.0 37.0 130-134 35.794442983365514 37.0 37.0 37.0 37.0 37.0 135-139 35.76464561495824 37.0 37.0 37.0 37.0 37.0 140-144 35.864617231903026 37.0 37.0 37.0 37.0 37.0 145-149 35.67189622349451 37.0 37.0 37.0 37.0 37.0 150 35.58224325076538 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 4.0 24 3.0 25 0.0 26 4.0 27 9.0 28 8.0 29 14.0 30 32.0 31 41.0 32 39.0 33 70.0 34 107.0 35 381.0 36 2968.0 37 316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.824999999999996 13.125 8.175 26.875 2 21.8523153942428 10.212765957446807 37.196495619524406 30.738423028785984 3 17.974999999999998 15.075 34.949999999999996 32.0 4 23.525 22.85 27.575 26.05 5 23.025000000000002 31.825 23.825 21.325 6 19.575 39.825 23.275000000000002 17.325 7 14.924999999999999 30.349999999999998 39.7 15.024999999999999 8 16.5 30.349999999999998 25.424999999999997 27.725 9 17.5 26.974999999999998 34.175 21.349999999999998 10-14 21.240000000000002 27.93 26.840000000000003 23.990000000000002 15-19 21.154999999999998 26.584999999999997 27.845 24.415 20-24 21.955 26.69 25.61 25.745 25-29 22.335 28.605000000000004 25.009999999999998 24.05 30-34 19.11 27.38 27.779999999999998 25.729999999999997 35-39 18.78 29.755 25.785000000000004 25.679999999999996 40-44 18.43 31.240000000000002 28.875 21.455 45-49 15.915000000000001 31.34 30.095 22.650000000000002 50-54 21.175 28.155 28.32 22.35 55-59 19.2 33.56 26.22 21.02 60-64 17.549999999999997 32.37 29.73 20.349999999999998 65-69 18.29 34.21 28.005000000000003 19.495 70-74 18.94784217632645 31.92978946842026 27.229084362654397 21.89328399259889 75-79 17.901636063441238 34.98774203232101 26.692350027517886 20.418271876719867 80-84 20.1904284640441 31.671260335755452 29.42119769481333 18.717113505387122 85-89 19.01228378039609 30.85485083980948 27.986964151416394 22.14590122837804 90-94 19.536074710046694 33.78520861575539 28.91499723853994 17.763719435657983 95-99 19.74406771121971 34.026903118545015 28.041714947856317 18.187314222378962 100-104 20.42434958322809 30.214700681990404 29.931800959838345 19.429148774943165 105-109 21.608473116099937 28.632240409466377 29.949830233618812 19.809456240814878 110-114 19.397296473218823 28.320967578005895 31.390385201748146 20.891350747027136 115-119 20.266188891732785 28.717686204248782 28.927565907345787 22.08855899667264 120-124 19.817450495049506 27.387582508250823 29.940181518151814 22.854785478547853 125-129 20.420921025213588 30.126067930818923 28.193373619504065 21.259637424463428 130-134 22.92688070008962 29.32679635194264 24.87743159892456 22.868891349043174 135-139 20.325246600906425 27.67262063449747 28.552386030391897 23.44974673420421 140-144 22.659165900362417 29.44231081300373 25.704549142640776 22.193974143993074 145-149 21.048854540436103 29.660502346121998 25.86254485233232 23.42809826110958 150 16.198163094906764 32.869468410798774 27.720567770665184 23.211800723629278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.5 18 0.5 19 0.5 20 1.5 21 1.5 22 1.5 23 1.0 24 4.0 25 9.5 26 11.5 27 13.5 28 23.0 29 28.0 30 30.5 31 57.0 32 78.5 33 80.0 34 110.0 35 114.0 36 110.5 37 178.0 38 187.0 39 155.0 40 184.5 41 215.5 42 242.0 43 267.5 44 353.5 45 346.0 46 231.0 47 216.5 48 172.5 49 159.5 50 160.0 51 79.5 52 29.5 53 21.0 54 17.0 55 16.5 56 15.5 57 9.0 58 19.5 59 17.0 60 4.0 61 2.5 62 3.5 63 4.5 64 7.5 65 7.0 66 2.5 67 0.5 68 0.0 69 0.0 70 0.0 71 1.0 72 1.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70-71 1.0 72-73 0.0 74-75 1.0 76-77 1.0 78-79 2.0 80-81 5.0 82-83 0.0 84-85 0.0 86-87 2.0 88-89 1.0 90-91 2.0 92-93 6.0 94-95 6.0 96-97 5.0 98-99 5.0 100-101 6.0 102-103 1.0 104-105 6.0 106-107 7.0 108-109 2.0 110-111 6.0 112-113 5.0 114-115 17.0 116-117 14.0 118-119 8.0 120-121 14.0 122-123 10.0 124-125 20.0 126-127 15.0 128-129 20.0 130-131 17.0 132-133 15.0 134-135 12.0 136-137 30.0 138-139 15.0 140-141 26.0 142-143 28.0 144-145 40.0 146-147 20.0 148-149 16.0 150-151 3593.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.525 #Duplication Level Percentage of deduplicated Percentage of total 1 47.67747589833479 13.600000000000001 2 18.054338299737072 10.299999999999999 3 10.429447852760736 8.924999999999999 4 6.222611744084137 7.1 5 2.5416301489921125 3.6249999999999996 6 3.1551270815074495 5.4 7 1.4899211218229622 2.9749999999999996 8 1.7528483786152498 4.0 9 1.0517090271691498 2.7 >10 7.099035933391762 30.975 >50 0.5258545135845749 10.4 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 89 2.225 No Hit GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 81 2.025 No Hit GTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 67 1.675 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 66 1.6500000000000001 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 57 1.425 No Hit GCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGG 56 1.4000000000000001 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 36 0.8999999999999999 No Hit GGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGG 31 0.775 No Hit GGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCC 31 0.775 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 26 0.65 No Hit AGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 26 0.65 No Hit CCCACATCCAACACCATGCAGAGTGTTCATGGACAGTCTTCTTTTTCTCC 25 0.625 No Hit CCAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGC 22 0.5499999999999999 No Hit GGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACG 22 0.5499999999999999 No Hit GCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATG 22 0.5499999999999999 No Hit GCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGA 21 0.525 No Hit GCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATAC 21 0.525 No Hit CTCCAATTAAGCTCTTTTTTCAGAATTTTAACAATCTCACGCCTTGGCAC 20 0.5 No Hit GTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCG 20 0.5 No Hit GCCAGACATGGTGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTG 20 0.5 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 19 0.475 No Hit CACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCATTTTTACGAAT 19 0.475 No Hit ATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCTTTGG 19 0.475 No Hit CTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCT 19 0.475 No Hit GCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAA 18 0.44999999999999996 No Hit GAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTAC 18 0.44999999999999996 No Hit CTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGC 17 0.42500000000000004 No Hit GCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGC 17 0.42500000000000004 No Hit CACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCT 16 0.4 No Hit GTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCA 16 0.4 No Hit GCTCAAAAGCCAAGGGGCAACCATTTTTACGAATTGCTTCATGTGTAGGC 16 0.4 No Hit ATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGA 16 0.4 No Hit GTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAA 16 0.4 No Hit GTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCC 16 0.4 No Hit GCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTATATG 16 0.4 No Hit ATACAATCTAGTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACC 15 0.375 No Hit ATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCT 15 0.375 No Hit GCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCTCAAA 15 0.375 No Hit ACTGGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTA 15 0.375 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 15 0.375 No Hit GTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAAT 15 0.375 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 15 0.375 No Hit CCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGG 15 0.375 No Hit CTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGA 14 0.35000000000000003 No Hit ATCGATTTGTTTGAGATCTACAAGATTAGAACTTTGTTCTAGCTCTATAG 14 0.35000000000000003 No Hit GTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCA 14 0.35000000000000003 No Hit GCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATA 14 0.35000000000000003 No Hit GTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAG 13 0.325 No Hit TACCACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTAC 13 0.325 No Hit GGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTC 13 0.325 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 13 0.325 No Hit CTCCAGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTG 13 0.325 No Hit GGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGC 13 0.325 No Hit GGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGC 13 0.325 No Hit CTCACATCAGTTCTGTTACTCTCACCACATCATCTGCCTAAAAAAAACAC 13 0.325 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 13 0.325 No Hit GTTAGTACCACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTA 13 0.325 No Hit GTCTTCAAGGACAATCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACC 13 0.325 No Hit GCTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGATCCA 13 0.325 No Hit GCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCG 13 0.325 No Hit GGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCATGCA 13 0.325 No Hit ATCGGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAA 12 0.3 No Hit GTAGGAAGTTCTCCAACTAACCCGATTCCACTCGCTAAAAAGATTTGCAG 12 0.3 No Hit GTCCTGTCCCCCCAGCCACACATGAGATCATAGTACGAACACGGAGAGAG 12 0.3 No Hit CACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCA 12 0.3 No Hit GTCTTCTTTTTCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAATCTCA 12 0.3 No Hit CCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAAC 11 0.27499999999999997 No Hit GGTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCA 11 0.27499999999999997 No Hit ATTGGCTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGA 11 0.27499999999999997 No Hit ATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAA 11 0.27499999999999997 No Hit TTTTTTTTTTCCAGAATAAAAGTCTTGCTTGATATTATCATTAAAAGCTG 11 0.27499999999999997 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 11 0.27499999999999997 No Hit GCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAAT 11 0.27499999999999997 No Hit GCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAG 11 0.27499999999999997 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 11 0.27499999999999997 No Hit CCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACAT 11 0.27499999999999997 No Hit CAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGAT 10 0.25 No Hit CTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCA 10 0.25 No Hit GCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCA 10 0.25 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 10 0.25 No Hit CCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAA 10 0.25 No Hit GTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTC 10 0.25 No Hit TGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTG 10 0.25 No Hit GTACCATTCCGATTGTACCTTATGACAACTCGAGTAATAGCATACGCTGG 10 0.25 No Hit GCTGACAACACTTATGACAACTCGAGTAATAGCATACGCTGGTTTTCCCA 10 0.25 No Hit CTCAGAAAAACCTTGTTCTTGACGCAGCCCAATCTTTATTAATGAGGAGA 10 0.25 No Hit TTCTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTG 10 0.25 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 9 0.22499999999999998 No Hit ATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAAT 9 0.22499999999999998 No Hit CACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATAA 9 0.22499999999999998 No Hit ACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAG 9 0.22499999999999998 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 9 0.22499999999999998 No Hit CTCATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCT 9 0.22499999999999998 No Hit CTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAG 9 0.22499999999999998 No Hit GCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 9 0.22499999999999998 No Hit CTTCATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTTTAACAAA 9 0.22499999999999998 No Hit CTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGATCCAA 9 0.22499999999999998 No Hit GCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAATTTGT 9 0.22499999999999998 No Hit GTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAA 9 0.22499999999999998 No Hit GTCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATT 8 0.2 No Hit TAGGAGGAATAGTATCGGGAACTGATGAGAGTTCCACTTTAAAATGGCTA 8 0.2 No Hit GTGGCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTG 8 0.2 No Hit CGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCT 8 0.2 No Hit ACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTATATGTACCT 8 0.2 No Hit GGACAGTCTTCTTTTTCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAA 8 0.2 No Hit CCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATA 8 0.2 No Hit CTGGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTAT 8 0.2 No Hit GTTCTTGACGCAGCCCAATCTTTATTAATGAGGAGAACTAAACATGAGTC 8 0.2 No Hit CTTTGTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAGATATTCTCTC 8 0.2 No Hit TTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCTC 8 0.2 No Hit CAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCA 8 0.2 No Hit GGCCATAGTATGGCTCGGGCACAGACCACCTTGCACTACGATATATCCCA 8 0.2 No Hit GCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGAT 8 0.2 No Hit GAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAA 8 0.2 No Hit GCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTT 8 0.2 No Hit AGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 8 0.2 No Hit GTCCAGTTATCTGAAGTTTTAAACCTATTATATGGTAACTTCAATGTGTT 8 0.2 No Hit TGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCT 8 0.2 No Hit GTGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCT 8 0.2 No Hit CTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATT 7 0.17500000000000002 No Hit CTCGGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCAT 7 0.17500000000000002 No Hit CGATTAAGTATGGTATAAAACAGGCGTCAGTTAAAGTTACCAAAATCCAG 7 0.17500000000000002 No Hit GCTTTTTCTTTTTACTGCTTGGGCTGGCATTTTGCATGTTCCCATCCAAG 7 0.17500000000000002 No Hit CTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAA 7 0.17500000000000002 No Hit GGCACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCTT 7 0.17500000000000002 No Hit GGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCT 7 0.17500000000000002 No Hit CATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAAC 7 0.17500000000000002 No Hit CCTATTTTTTCCTCAAATAATCATCTCGCTGAGCTGGATTGAGTCCTAAA 7 0.17500000000000002 No Hit GCTCAGTTGTACACTTGTATGCCAGCGGTTTCATCACTAGCATCCATCTT 7 0.17500000000000002 No Hit CTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTT 7 0.17500000000000002 No Hit CTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCT 7 0.17500000000000002 No Hit GTATGGTATAAAACAGGCGTCAGTTAAAGTTACCAAAATCCAGTGAGACA 7 0.17500000000000002 No Hit AAACTTGTAACAACGCTCAGTTGTACACTTGTATGCCAGCGGTTTCATCA 7 0.17500000000000002 No Hit GTGCTCTAGGAGGAATAGTATCGGGAACTGATGAGAGTTCCACTTTAAAA 7 0.17500000000000002 No Hit GCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCCT 7 0.17500000000000002 No Hit GCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTT 7 0.17500000000000002 No Hit TGGGCACAGACCACCTTGCACTACGATATATCCCACATCCAACACCATGC 6 0.15 No Hit AGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAAATA 6 0.15 No Hit CCTGCTGCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATG 6 0.15 No Hit GGCAGAGATAGCAGTTCACATTCTTCCTTTTTCATGAAAAAAAAGGGTTC 6 0.15 No Hit GCCACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCATTTTTACGA 6 0.15 No Hit GTCTTTTTTCCTGATCGATGCGTGCGGACCTTTTTGGTGCGACAAAATTT 6 0.15 No Hit CCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCTGTCCCCCCAGCCAC 6 0.15 No Hit GTCCTCGAGAGCATCTGAAATTCCAGGTATGGCAGAAATATCACCACCAA 6 0.15 No Hit CCATCAACGGCCCCTTCCATCTCCTCAGAGATATCATCGTCATACTGAAG 6 0.15 No Hit GCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCTCAAAAGCCAAG 6 0.15 No Hit CGGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTATA 6 0.15 No Hit GTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGC 6 0.15 No Hit CCATGGTGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGG 6 0.15 No Hit CGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATA 6 0.15 No Hit CGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTC 6 0.15 No Hit GCTGGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTA 6 0.15 No Hit GCCAAGGGGCAACCATTTTTACGAATTGCTTCATGTGTAGGCAGAGGAAA 6 0.15 No Hit ACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTT 6 0.15 No Hit GCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCA 6 0.15 No Hit ATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTATATGTACCTAT 6 0.15 No Hit TTTCTCCAATTAAGCTCTTTTTTCAGAATTTTAACAATCTCACGCCTTGG 6 0.15 No Hit CACCATTCCAGCATCAACTTTCCATATAATCCTAAAGCTAGTAAACACAC 6 0.15 No Hit ATCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATT 6 0.15 No Hit CTCTTTTTTCAGAATTTTAACAATCTCACGCCTTGGCACGAAAAGAGTTT 6 0.15 No Hit CCTTGCACTACGATATATCCCACATCCAACACCATGCAGAGTGTTCATGG 6 0.15 No Hit GTGACGGTACTCGAGGCTCCTCTTGAGACCATGACGAAAAAACTGGGGGT 6 0.15 No Hit CAAACGTATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTG 6 0.15 No Hit CCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGAT 6 0.15 No Hit ACTCATGATTAGTCCAGAAATAGAACGAAAAATAGCGACAATATTTTTTC 6 0.15 No Hit GCCATAGTATGGCTCGGGCACAGACCACCTTGCACTACGATATATCCCAC 6 0.15 No Hit GGACAATCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACC 6 0.15 No Hit GGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTATAT 6 0.15 No Hit AAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATT 6 0.15 No Hit TGGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTG 6 0.15 No Hit GCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCAG 6 0.15 No Hit AGCTTATAAAAAAAATTCAATTTTCACTCAGAAAAACCTTGTTCTTGACG 6 0.15 No Hit GGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTC 5 0.125 No Hit TGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAG 5 0.125 No Hit GGCCCTTATAGGCAGTTTTACTAAAGTGAGAAGTGGCACTACTGACTGCA 5 0.125 No Hit CCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACCATTTTTACGAATTG 5 0.125 No Hit AGAAAATTATCGATTTGTTTGAGATCTACAAGATTAGAACTTTGTTCTAG 5 0.125 No Hit CTGCTGTCCAATTATATATTGCGCTAGCACACAACTGTCCAAAAGACAAT 5 0.125 No Hit GTATAAAACAGGCGTCAGTTAAAGTTACCAAAATCCAGTGAGACATCATA 5 0.125 No Hit GAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCC 5 0.125 No Hit CCTGGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTA 5 0.125 No Hit CATGGCCATAGTATGGCTCGGGCACAGACCACCTTGCACTACGATATATC 5 0.125 No Hit CAGGTTTCTGGGATGGCGGATAGCATCAGATATTCTCTCTGCATGATTTT 5 0.125 No Hit GGAAACTTGTAACAACGCTCAGTTGTACACTTGTATGCCAGCGGTTTCAT 5 0.125 No Hit CTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCCTCAAATAA 5 0.125 No Hit GCCTACAGAAAATTATCGATTTGTTTGAGATCTACAAGATTAGAACTTTG 5 0.125 No Hit GGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTA 5 0.125 No Hit GCAACGAGACCCTTATAGGCAGTTTTACTAAAGTGAGAAGTGGCACTACT 5 0.125 No Hit GCTCGCTCAGCACGCACCACCCGCTGCCGGCTTCGTCTTTTTTCCTGATC 5 0.125 No Hit GGGCTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGC 5 0.125 No Hit GCACGCAGATCTTACCCCCCTCCAGGCACCGACACTGCCATGGCTGTCCT 5 0.125 No Hit GGATCTGAAAGAATGTCTTCAAGGACAATCGGCTTCGCTAGTTGCACTGT 5 0.125 No Hit CAATAAGATTTTTTGAACCTTGTGCGATACCATTTAATAGCTCAACAGGA 5 0.125 No Hit GACGCCCCTGCAACTTGCAAGTTTGCTCATTCTCTGTAGTGCACATGCAT 5 0.125 No Hit GTGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAG 5 0.125 No Hit ATGTAGTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCT 5 0.125 No Hit GCTTGATCTTTCCTAAAACAGTAGATTTTTTTGTGTGTTATTCACAAGAT 5 0.125 No Hit CTTACAGTTACAGTTTCACCAGTGGCTTTTAATTGCAAGGTCTCTGCAGA 5 0.125 No Hit TCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCG 5 0.125 No Hit ACTAGGAGGAATAGTATCGGGAACTGATGAGAGTTCCACTTTAAAATGGC 5 0.125 No Hit CAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTGGCTAACATAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR5262807 read2 length is 71-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262807_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 71-150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1105 37.0 37.0 37.0 37.0 37.0 2 35.899 37.0 37.0 37.0 37.0 37.0 3 35.984 37.0 37.0 37.0 37.0 37.0 4 36.071 37.0 37.0 37.0 37.0 37.0 5 36.174 37.0 37.0 37.0 37.0 37.0 6 36.114 37.0 37.0 37.0 37.0 37.0 7 36.0765 37.0 37.0 37.0 37.0 37.0 8 36.2205 37.0 37.0 37.0 37.0 37.0 9 36.165 37.0 37.0 37.0 37.0 37.0 10-14 36.259 37.0 37.0 37.0 37.0 37.0 15-19 36.2285 37.0 37.0 37.0 37.0 37.0 20-24 36.1387 37.0 37.0 37.0 37.0 37.0 25-29 36.1759 37.0 37.0 37.0 37.0 37.0 30-34 36.169 37.0 37.0 37.0 37.0 37.0 35-39 36.08630000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.1228 37.0 37.0 37.0 37.0 37.0 45-49 36.1303 37.0 37.0 37.0 37.0 37.0 50-54 35.998599999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.9645 37.0 37.0 37.0 37.0 37.0 60-64 35.9337 37.0 37.0 37.0 37.0 37.0 65-69 35.9149 37.0 37.0 37.0 37.0 37.0 70-74 35.88063230807702 37.0 37.0 37.0 37.0 37.0 75-79 35.8505691648037 37.0 37.0 37.0 37.0 37.0 80-84 35.808403863225024 37.0 37.0 37.0 37.0 37.0 85-89 35.8113296689437 37.0 37.0 37.0 37.0 37.0 90-94 35.7194409746548 37.0 37.0 37.0 37.0 37.0 95-99 35.698682556977396 37.0 37.0 37.0 37.0 37.0 100-104 35.62559322436051 37.0 37.0 37.0 37.0 37.0 105-109 35.58250311356214 37.0 37.0 37.0 37.0 37.0 110-114 35.608485946614024 37.0 37.0 37.0 37.0 37.0 115-119 35.48995002228546 37.0 37.0 37.0 37.0 37.0 120-124 35.5371744520795 37.0 37.0 37.0 37.0 37.0 125-129 35.39323539874883 37.0 37.0 37.0 34.6 37.0 130-134 35.380784008568185 37.0 37.0 37.0 34.6 37.0 135-139 35.357691964262884 37.0 37.0 37.0 32.2 37.0 140-144 35.29672181918248 37.0 37.0 37.0 29.8 37.0 145-149 35.21924540021563 37.0 37.0 37.0 29.8 37.0 150 35.20471567267684 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 4.0 21 5.0 22 3.0 23 5.0 24 4.0 25 8.0 26 8.0 27 11.0 28 15.0 29 15.0 30 33.0 31 32.0 32 67.0 33 128.0 34 258.0 35 626.0 36 2574.0 37 200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.825 22.175 9.375 21.625 2 30.55 23.025000000000002 28.575 17.849999999999998 3 21.6 25.974999999999998 34.325 18.099999999999998 4 23.45 28.775000000000002 27.700000000000003 20.075000000000003 5 25.025 34.2 22.825 17.95 6 22.25 36.1 22.45 19.2 7 23.325000000000003 21.2 36.375 19.1 8 21.475 24.425 28.7 25.4 9 19.275000000000002 25.275 31.3 24.15 10-14 24.37 26.650000000000002 26.810000000000002 22.17 15-19 24.16 26.224999999999998 27.975 21.64 20-24 24.03 27.49 26.6 21.88 25-29 24.560000000000002 26.015 28.74 20.685000000000002 30-34 23.474999999999998 27.425 27.855 21.245 35-39 23.815 27.305 27.22 21.66 40-44 24.32 26.035000000000004 28.139999999999997 21.505 45-49 23.02 26.815 29.29 20.875 50-54 22.955000000000002 28.16 28.26 20.625 55-59 23.330000000000002 27.18 28.68 20.810000000000002 60-64 22.495 26.895000000000003 29.054999999999996 21.555 65-69 22.595000000000002 26.6 28.665000000000003 22.14 70-74 23.66855028254238 26.143921588238232 29.919487923188477 20.268040206030904 75-79 22.919897933656877 26.43718416971031 30.024515935357982 20.61840196127483 80-84 24.16938110749186 27.191180155349535 29.255825607617137 19.38361312954147 85-89 23.57483078465781 27.851591877663573 27.56079217849085 21.012785159187768 90-94 23.542702214188886 29.025455640909776 27.82547572425566 19.606366420645678 95-99 24.107007909718373 27.48753085797773 27.729356642652025 20.67610458965187 100-104 23.8545087143218 26.759282647133116 29.72972972972973 19.65647890881536 105-109 22.931130593422186 28.064663254446863 29.062990928900827 19.94121522323012 110-114 23.42717755869499 27.548531354812482 29.0629128976522 19.961378188840328 115-119 23.85461991297671 27.361146659841314 28.973637061684155 19.810596365497823 120-124 23.70565181518152 27.475247524752476 28.8263201320132 19.992780528052805 125-129 23.61429464471765 26.797249426963948 28.95394873932069 20.63450718899771 130-134 24.065580684274344 27.20754915915441 29.163371817175392 19.563498339395856 135-139 23.44974673420421 27.50199946680885 28.333777659290853 20.71447613969608 140-144 21.69704180412092 27.218646909307232 30.090314206911472 20.993997079660375 145-149 22.74578883628757 26.57161730705714 29.98458659033359 20.6980072663217 150 22.385575589459084 28.349514563106798 27.489597780859913 21.7753120665742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.5 21 0.5 22 0.0 23 0.0 24 0.5 25 3.0 26 4.5 27 5.0 28 8.5 29 16.0 30 24.0 31 22.0 32 21.0 33 36.0 34 54.0 35 61.0 36 101.5 37 138.5 38 151.5 39 172.0 40 174.0 41 207.5 42 236.5 43 257.5 44 308.0 45 324.5 46 278.5 47 226.5 48 196.5 49 163.0 50 130.0 51 98.5 52 76.5 53 64.0 54 46.5 55 31.5 56 25.0 57 33.5 58 49.0 59 35.5 60 20.0 61 21.5 62 19.5 63 18.5 64 17.0 65 10.5 66 6.5 67 4.5 68 14.5 69 20.5 70 12.0 71 11.0 72 10.0 73 5.5 74 7.0 75 7.0 76 3.0 77 3.5 78 4.0 79 2.0 80 1.0 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70-71 1.0 72-73 0.0 74-75 1.0 76-77 1.0 78-79 2.0 80-81 5.0 82-83 0.0 84-85 0.0 86-87 2.0 88-89 1.0 90-91 2.0 92-93 6.0 94-95 6.0 96-97 5.0 98-99 5.0 100-101 6.0 102-103 1.0 104-105 6.0 106-107 7.0 108-109 2.0 110-111 6.0 112-113 5.0 114-115 17.0 116-117 14.0 118-119 8.0 120-121 14.0 122-123 10.0 124-125 20.0 126-127 15.0 128-129 20.0 130-131 17.0 132-133 15.0 134-135 12.0 136-137 30.0 138-139 17.0 140-141 23.0 142-143 26.0 144-145 31.0 146-147 20.0 148-149 16.0 150-151 3605.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.449999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 55.10835913312694 26.700000000000003 2 20.94943240454076 20.3 3 10.113519091847266 14.7 4 5.779153766769865 11.200000000000001 5 3.0959752321981426 7.5 6 1.7543859649122806 5.1 7 0.9803921568627451 3.325 8 0.7223942208462332 2.8000000000000003 9 0.5675954592363261 2.475 >10 0.9287925696594427 5.8999999999999995 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 29 0.7250000000000001 No Hit GGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTC 26 0.65 No Hit GCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACAT 15 0.375 No Hit ATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCA 13 0.325 No Hit TGGTAGGTCAACAACTCAATTCAACTTATAGTTTTCAAGAGATATACACA 13 0.325 No Hit CCTAAATTAAGTTACAGGTCGTGGGTTTTCATAGCTCGGATATGTGTGAC 12 0.3 No Hit GTGGCTACAACCGGAATAGAGAATTCGATGCCCAATTTAAGTGTGAGGAA 12 0.3 No Hit GTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGA 12 0.3 No Hit TGTAGAATCAAGAAGGCTGGGATTGGAGGCCCCCTTATTAATTTTGACGG 12 0.3 No Hit GAGAAGGCCAAATCCCTACAGTCTATTGCTATTGACGCGAACGCGTCATC 11 0.27499999999999997 No Hit GTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGA 11 0.27499999999999997 No Hit TTTAAGTCGCCTAGTTGCTATGCGTTACCAAGCCAAACTAGGGATCATGG 10 0.25 No Hit CTCCACTCCACTCTCTCTCTCTCTGTTCGCCTCTCGCCTTTTTAGAGAGA 10 0.25 No Hit CAATAAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTC 10 0.25 No Hit GGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTCAA 10 0.25 No Hit GGATGAGCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAA 10 0.25 No Hit GGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAAC 10 0.25 No Hit AGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCT 10 0.25 No Hit GAAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAA 9 0.22499999999999998 No Hit GCTTTAAGTCGCCTAGTTGCTATGCGTTACCAAGCCAAACTAGGGATCAT 9 0.22499999999999998 No Hit GAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTT 9 0.22499999999999998 No Hit CTGCTGGTGTCATAGGACATAAGCTATGCATATGTGCTTCTTTTAAATTT 9 0.22499999999999998 No Hit GGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGG 9 0.22499999999999998 No Hit GTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTT 9 0.22499999999999998 No Hit GTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTAT 9 0.22499999999999998 No Hit GGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAA 9 0.22499999999999998 No Hit GTTGCTGAAGTGTTCACAGGTGCACCTGGAAAGTATGTTGAACTAAAGGA 9 0.22499999999999998 No Hit GAATTCGATGCCCAATTTAAGTGTGAGGAAAACAAGGAGATCATTTTCTT 9 0.22499999999999998 No Hit GCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCG 9 0.22499999999999998 No Hit CTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTT 8 0.2 No Hit GGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAG 8 0.2 No Hit CTTCATCAAGTTTACACAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCT 8 0.2 No Hit GTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATT 8 0.2 No Hit GTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCC 8 0.2 No Hit GAGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATC 8 0.2 No Hit GACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGACG 8 0.2 No Hit CGCCGAGAAGGGGAACGGAAGCGCCGACCAGAACCGAGGGCGAGCGGCGG 8 0.2 No Hit GATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGGTCAT 8 0.2 No Hit ACGTGGAGTACGAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAG 8 0.2 No Hit TGATAAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTAGCC 8 0.2 No Hit GCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGC 8 0.2 No Hit GTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGA 8 0.2 No Hit AGAAGGTTCTCCAGAATTACAAGAATCTTCAAGATATTATTGCCATTTTG 8 0.2 No Hit AGGTTATTGAAGTGTTGCGGGGGAGACGGTCTGATTTAAAAAGCTACCAT 7 0.17500000000000002 No Hit GCTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAA 7 0.17500000000000002 No Hit GAATGGATGAGCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGG 7 0.17500000000000002 No Hit ACAGATTTGACACAGAATACTTGCTCGGATGTCGTTCTCGAGTACTTGCG 7 0.17500000000000002 No Hit AGTGGCTATAGGGCGTCAAATAAGTGAAGGATTTCTAATGGCCACAGAAG 7 0.17500000000000002 No Hit GGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAG 7 0.17500000000000002 No Hit GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 7 0.17500000000000002 No Hit TGAAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCA 7 0.17500000000000002 No Hit CACGAAACCATAGACTTTGACGTGCATGTTGGTCATTTTTCATATTTCGA 7 0.17500000000000002 No Hit GGGCCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGAT 7 0.17500000000000002 No Hit ATTGAAGTTTGCTGATGCAACACTTGCAAGATATGCTTTAAGTCGCCTAG 7 0.17500000000000002 No Hit AATAAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTCA 7 0.17500000000000002 No Hit CGTTCTCGAGTACTTGCGTCCACTTGTAGAATCAAGAAGGCTGGGATTGG 7 0.17500000000000002 No Hit AGGGAATCCTATGGGTTTTGCCAGGAATGTTGGGTTTGGTTTAAAAGACT 7 0.17500000000000002 No Hit GGGAGGAGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCAT 7 0.17500000000000002 No Hit AGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTT 7 0.17500000000000002 No Hit GGCCCCATTATCTCTGAAAAACATTTCTGAGGGGTGTGCTACTTTCATGC 7 0.17500000000000002 No Hit CAGAGATTTGAGAGCTACTTCTACGTGCTACTGAGGACTGATTGAGGCTT 7 0.17500000000000002 No Hit GTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCA 7 0.17500000000000002 No Hit GTATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGT 6 0.15 No Hit GTGGAGTACGAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGC 6 0.15 No Hit CAAAACTGACTCTTGGTACTGTTGCACCACAGTTTACTGGTATTTCTATC 6 0.15 No Hit CAGAAGCTTGGTTTGTCTCGGACAGAGTCGGATCCTTCTCCAGTGAAGGC 6 0.15 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 6 0.15 No Hit GTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAAT 6 0.15 No Hit ATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGA 6 0.15 No Hit GGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTCGTG 6 0.15 No Hit GGTGAATTAGGTGTAACAACATCTAGCAAGCATTGATATCTTAATTTCTT 6 0.15 No Hit GCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCAT 6 0.15 No Hit ATTTAAACAATGGCTCGATCAGTTTAGATACATTGATGTGTTTGTACTTT 6 0.15 No Hit GCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGAACAGCCATGCTGGTG 6 0.15 No Hit CTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTCGTGACAA 6 0.15 No Hit AGAGAATGAGAATCAAATGAGAGAGAAAACTGACAATGCCTTGGTTCATT 6 0.15 No Hit GATGAGCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAAT 6 0.15 No Hit CTTTAAAACCCACACCAAACCCTTTGAGACCCAGTACAATTGAAAAAGAT 6 0.15 No Hit TGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGAGGGGTTC 6 0.15 No Hit GTTGTGTTTGCACAGTGAGCCAGTGAGGTAGCCTGGTGCCCCGCGCTAAA 6 0.15 No Hit GTTAACAGTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTAGCCAGCCTT 6 0.15 No Hit GTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCC 6 0.15 No Hit AGAAAGAGCGCGCAGAATAAATGAAGTTCTAGAAGTGCATGTTGACCCCC 6 0.15 No Hit CTTTGGACTCCACATCCAGAATGTTGTCTCCCCACGTTCTTGGTGAGGAG 6 0.15 No Hit GTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTAGCCAGCCTTTTCATGT 6 0.15 No Hit GTTCACAGGTGCACCTGGAAAGTATGTTGAACTAAAGGAGAGTGTTCAAA 6 0.15 No Hit GCTAATCAAAGTCCGGTAGGTGACTTGGCACTAGCTACCCTTGAGGACCA 6 0.15 No Hit CTACATGGAAAACGTCCTTGATATATTGAAGTTTGCTGATGCAACACTTG 6 0.15 No Hit GCCTGATGTAATTTATCCATCATCTCTGCATTTTTTTTATGGTTTACATC 6 0.15 No Hit GGTTTCCATTGCATTCGTCCATTTGATATCTGCTGTAAAGAAGATGATTT 6 0.15 No Hit GTGTATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATG 6 0.15 No Hit AAACCCTTTGAGACCCAGTACAATTGAAAAAGATCCACTGGCTCATTTGA 6 0.15 No Hit ACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCCAC 6 0.15 No Hit GGATGTTGTCATGCTGGATGATGACGAAGACTTGCATGATGCAGCTATCA 6 0.15 No Hit GGGGATGTTGTCATGCTGGATGATGACGAAGACTTGCATGATGCAGCTAT 6 0.15 No Hit CTTTTAGTAGTATGGGACAAAGTCTTCGCTTGTTGCAACAATATGCTTCT 6 0.15 No Hit AGTACTTGCGTCCACTTGTAGAATCAAGAAGGCTGGGATTGGAGGCCCCC 5 0.125 No Hit ATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTGCTC 5 0.125 No Hit GCGGAAGATACTACCACTTCCTGGTGATGTCGATGTTGTATGTGGAGGTC 5 0.125 No Hit CCTCTGTGTGCTGCCCTTCTTGCGCCCGGCTGCGGGTATCCGCTTCGTGA 5 0.125 No Hit CCAGTCCAATTCAGTTCAGCCAATTGTCAATGTCGAGGAGAAGTTTCATG 5 0.125 No Hit TGTCGATGTTGTATGTGGAGGTCCACCATGCCAAGGTATTAGTGGCTACA 5 0.125 No Hit AAGGTAAGAACATTTTTCATTGTACAAACTTGCCGTAGCTAATTTCAGTT 5 0.125 No Hit GAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCG 5 0.125 No Hit GAGGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTC 5 0.125 No Hit GGATGATAAGTTAACAGTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTA 5 0.125 No Hit AAAATACGTCTACATGGAAAACGTCCTTGATATATTGAAGTTTGCTGATG 5 0.125 No Hit TGAAGTTTGCTGATGCAACACTTGCAAGATATGCTTTAAGTCGCCTAGTT 5 0.125 No Hit GCAAGGAGAGAATGAGAATCAAATGAGAGAGAAAACTGACAATGCCTTGG 5 0.125 No Hit AGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTT 5 0.125 No Hit GGATACATGATGGAGGCGCTGAGTTGGAGAACGGCGTGCCTCGTGGTCCT 5 0.125 No Hit TGCCTTATGTTAAGCTTCAGAAATCCAGAAAGAGGGAGTGAGTTCTTTGA 5 0.125 No Hit CCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTCATTTTTCAT 5 0.125 No Hit CTTGATATATTGAAGTTTGCTGATGCAACACTTGCAAGATATGCTTTAAG 5 0.125 No Hit GCAGCTATCAAGCAGGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAAA 5 0.125 No Hit TTTTTGTTCAAAAGTAGCAACACGCTCGATCGAGGAAGGCACGCACGTAT 5 0.125 No Hit CACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAGACTT 5 0.125 No Hit TGTGGCCTTAAGATTCACACCGAACACTCAAACAGGACCTTCCATGTGAA 5 0.125 No Hit CAGTTATTGATGAAGCTTTAAAACCCACACCAAACCCTTTGAGACCCAGT 5 0.125 No Hit TGTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCA 5 0.125 No Hit GCCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGA 5 0.125 No Hit GAGTTGTGTTTGCACAGTGAGCCAGTGAGGTAGCCTGGTGCCCCGCGCTA 5 0.125 No Hit GCTTTATTTAAATTATCATGCATTTCCTTTGTTTCTCCTTTTTCTGTGAA 5 0.125 No Hit CCACTTGTAGAATCAAGAAGGCTGGGATTGGAGGCCCCCTTATTAATTTT 5 0.125 No Hit AGACCTTTTGTTAAATATAATTAACATTGCTAAATACATTTCTGGCAGCC 5 0.125 No Hit GCGTGAGTCCGTTCTCTCCCCCATCCCCTGCACACTATTTTTTCCTATGA 5 0.125 No Hit GGGTGATGTCGATGTTGTATGTGGAGGTCCACCATGCCAAGGTATTAGTG 5 0.125 No Hit AGAGCACCCAAGGTTATTGAAGTGTTGCGGGGGAGACGGTCTGATTTAAA 5 0.125 No Hit GGAAAACGTCCTTGATATATTGAAGTTTGCTGATGCAACACTTGCAAGAT 5 0.125 No Hit CTCCACTCTCTCTCTCTCTGTTCGCCTCTCGCCTTTTTAGAGAGAGAAAG 5 0.125 No Hit GAACCACTATAATGAACGTAAAGAAACTCTTTTCGTGCCAAGGCGTGAGA 5 0.125 No Hit GGCACATTACCCTTACCGCGGACAAACAGATGTCGAGGCAGCATCTTCCC 5 0.125 No Hit GGCATGTACATGTGGTGGGGCTTGCAGGCGCGCGCTATGCCACCAGCGAA 5 0.125 No Hit AGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACCCA 5 0.125 No Hit AAAATTAAACAGTTTACAGTTGATTTAACTTCAGTGGAGACCAATATGGT 5 0.125 No Hit GCCGTGGCATTATAGCGAGGATGGGGTGGATCTTGTTGTTAAAGATCCTA 5 0.125 No Hit GAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCCAAGGTTATTGAA 5 0.125 No Hit TGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGA 5 0.125 No Hit AGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATCC 5 0.125 No Hit GAAACCATAGACTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAACA 5 0.125 No Hit GCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAA 5 0.125 No Hit GTTTTTGAACTAGCAGCAGAGACGGTGATGTTAGCTTCTGGTGTTGCTAT 5 0.125 No Hit CAAGCAGACCTCCTTGCGGACCTTTTGGGTCCTCTTGCAATAGAGGGTCC 5 0.125 No Hit GGAATCGGAAGAGCTAAAAGAAGGGTAGAAACTGAGAACCTTGCATCTAC 5 0.125 No Hit GGGGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCAT 5 0.125 No Hit GACATGTTTCGTCGGTATAGAAAGTTAACTTGCTGTGAGCTTCTATATTG 5 0.125 No Hit GTTGGGTTTGGTTTAAAAGACTTCATGTCTGCCTCGCGGAAAGGGAAATT 5 0.125 No Hit GGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACGCCGTG 5 0.125 No Hit GTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAGA 5 0.125 No Hit AGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCT 5 0.125 No Hit GGGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCT 5 0.125 No Hit GCCAAACTAGGGATCATGGCTGCTGGATGTTATGGCGTTCCACAATTTCG 5 0.125 No Hit CAGAATCTCGCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCATGAAGAA 5 0.125 No Hit GCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCATGAAGAACTCCATTCC 5 0.125 No Hit GCGTTACCAAGCCAAACTAGGGATCATGGCTGCTGGATGTTATGGCGTTC 5 0.125 No Hit CTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAACAACATGCCAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGAAT 10 0.007204877 142.4375 1 >>END_MODULE Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822992 spots for ERR5262807.sra Written 1822992 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra Read 1822979 spots for ERR5262807.sra Written 1822979 spots for ERR5262807.sra SRR ids: ['ERR5262807.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6surc61h ERR5262807.sra spots: 36459593 blocks: [[1, 1822979], [1822980, 3645958], [3645959, 5468937], [5468938, 7291916], [7291917, 9114895], [9114896, 10937874], [10937875, 12760853], [12760854, 14583832], [14583833, 16406811], [16406812, 18229790], [18229791, 20052769], [20052770, 21875748], [21875749, 23698727], [23698728, 25521706], [25521707, 27344685], [27344686, 29167664], [29167665, 30990643], [30990644, 32813622], [32813623, 34636601], [34636602, 36459593]] ERR5262807 file size 12013353 ERR5262807 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262807 ERR5262807_1.fastq ERR5262807_2.fastq Input file: ERR5262807_1.fastq Paired file: ERR5262807_2.fastq trimmed: ERR5262807-trimmed-pair1.fastq, ERR5262807-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:20:23 2024 >> started Fri Dec 6 12:21:18 2024 >> done (55.262s) 36459593 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 36459592 (100.00%) read pairs available; of these: 9857 ( 0.03%) trimmed read pairs available after processing 36449735 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 2 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 2 0.00% 25 0 0.00% 26 2 0.00% 27 1 0.00% 28 3 0.00% 29 1 0.00% 30 0 0.00% 31 4 0.00% 32 1 0.00% 33 1 0.00% 34 5 0.00% 35 4 0.00% 36 2 0.00% 37 2 0.00% 38 4 0.00% 39 4 0.00% 40 2 0.00% 41 1 0.00% 42 3 0.00% 43 2 0.00% 44 0 0.00% 45 2 0.00% 46 4 0.00% 47 4 0.00% 48 5 0.00% 49 302 0.00% 50 344 0.00% 51 318 0.00% 52 401 0.00% 53 393 0.00% 54 426 0.00% 55 482 0.00% 56 514 0.00% 57 615 0.00% 58 714 0.00% 59 887 0.00% 60 948 0.00% 61 1110 0.00% 62 1352 0.00% 63 1417 0.00% 64 1547 0.00% 65 1594 0.00% 66 1852 0.01% 67 2113 0.01% 68 2368 0.01% 69 2717 0.01% 70 3331 0.01% 71 3707 0.01% 72 4280 0.01% 73 4746 0.01% 74 5247 0.01% 75 5952 0.02% 76 6442 0.02% 77 7129 0.02% 78 7624 0.02% 79 8738 0.02% 80 9539 0.03% 81 11118 0.03% 82 12360 0.03% 83 13630 0.04% 84 14793 0.04% 85 15814 0.04% 86 17142 0.05% 87 18121 0.05% 88 19608 0.05% 89 20650 0.06% 90 22477 0.06% 91 24308 0.07% 92 26479 0.07% 93 28557 0.08% 94 30579 0.08% 95 32786 0.09% 96 33836 0.09% 97 35692 0.10% 98 36475 0.10% 99 37635 0.10% 100 39701 0.11% 101 42073 0.12% 102 44446 0.12% 103 47111 0.13% 104 49593 0.14% 105 51759 0.14% 106 53738 0.15% 107 54928 0.15% 108 56673 0.16% 109 58147 0.16% 110 60053 0.16% 111 62369 0.17% 112 64581 0.18% 113 66219 0.18% 114 70895 0.19% 115 72715 0.20% 116 74767 0.21% 117 76993 0.21% 118 78741 0.22% 119 78707 0.22% 120 80304 0.22% 121 82967 0.23% 122 82985 0.23% 123 87619 0.24% 124 90631 0.25% 125 91635 0.25% 126 94555 0.26% 127 96360 0.26% 128 98073 0.27% 129 98728 0.27% 130 100157 0.27% 131 100255 0.27% 132 102419 0.28% 133 105216 0.29% 134 108064 0.30% 135 110974 0.30% 136 112234 0.31% 137 114166 0.31% 138 115456 0.32% 139 118399 0.32% 140 119442 0.33% 141 122331 0.34% 142 124229 0.34% 143 125441 0.34% 144 129021 0.35% 145 129582 0.36% 146 134272 0.37% 147 315977 0.87% 148 124786 0.34% 149 124839 0.34% 150 31272995 85.77% 36459592 reads passed initial QC criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=2.17 fanout-score-rank=43 prefix-density=0.21 prefix-fanout=2.1 sequence=CCCTTGAAACCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=317.97 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=9.5 sequence=CCTTCTCCAGTCCAGCTCTTTCAAACTCGTTCAGTGGACCAAGTGGCAAGATCTCCTCAACGCCACATCGACCTAGGCGCACTTTGGATGCAAAGAAAGGCAGCTCCGTCACCTGAGAAGCTACATAAGAGCACTCTACTATCCCAGCATCACCATGCAATCCTCTTAAGCAAGCATCTGCGAATTTTGCTGCTGCATACGCCATAGAAAGAGTTGCTGATCCT criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.56 fanout-score-rank=37 prefix-density=0.34 prefix-fanout=2.3 sequence=ACCTTGTCAAGA criterion=fanout-score sequence-density=0.11 sequence-density-rank=14 fanout-score=126.53 fanout-score-rank=1 prefix-density=0.78 prefix-fanout=18.7 sequence=CGCCGCCGCCGC ERR5262807 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:21:59 Started mapping on | Dec 06 12:22:00 Finished on | Dec 06 12:24:25 Mapping speed, Million of reads per hour | 905.20 Number of input reads | 36459592 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 34984703 Uniquely mapped reads % | 95.95% Average mapped length | 291.91 Number of splices: Total | 31604114 Number of splices: Annotated (sjdb) | 29212684 Number of splices: GT/AG | 31118836 Number of splices: GC/AG | 402365 Number of splices: AT/AC | 17472 Number of splices: Non-canonical | 65441 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.24 Insertion rate per base | 0.01% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 377880 % of reads mapped to multiple loci | 1.04% Number of reads mapped to too many loci | 1165 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.00% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1097009 1097009 1097009 N_multimapping 377880 377880 377880 N_noFeature 1627164 33922866 1999397 N_ambiguous 809407 5115 119884 UnstrandedReadsAssigned:32548132 PositiveStrandReadsAssigned:1056722 NegativeStrandReadsAssigned:32865422 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262807 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262807-trimmed-pair1.fastq ERR5262807-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 36,459,592 reads, 33,252,571 reads pseudoaligned [quant] estimated average fragment length: 276.899 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,204 rounds 52973 ERR5262807.ke.tsv 35125 ERR5262807.se.tsv 88098 total ==> ERR5262807.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 660.871 0 0 PNS24247 1044 768.101 241.316 13.1346 PNS24249 1928 1652.1 506.978 12.8292 PNS24246 1044 768.101 241.316 13.1346 PNS24248 1044 768.101 241.316 13.1346 PNS24244 1471 1195.1 262.074 9.16784 PNS24243 293 99.5538 2 0.839885 KQK14069 1603 1327.1 81595.8 2570.47 KQK14071 474 232.059 516.629 93.0739 ==> ERR5262807.se.tsv <== BRADI_1g14170v3 83316 BRADI_1g53295v3 369 BRADI_1g59795v3 1186 BRADI_1g07683v3 0 BRADI_1g00485v3 19 BRADI_1g20270v3 441 BRADI_1g74790v3 3431 BRADI_1g09890v3 0 BRADI_1g77505v3 358 BRADI_1g48960v3 0 ERR5262807 completed mapping pipeline successfully