Starting /dee2/code/volunteer_pipeline.sh ERR5262808 current disk space = 1551413514240 free memory = 1605381336 ERR5262808 SRAfilesize 1913c8f63ed7185ada2f5d8d89384729 ERR5262808.sra ERR5262808.sra file validated ERR5262808 is paired end ERR5262808 is conventional basespace ERR5262808 read1 length is 49-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262808_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 49-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6115 37.0 37.0 37.0 37.0 37.0 2 36.47875 37.0 37.0 37.0 37.0 37.0 3 36.6615 37.0 37.0 37.0 37.0 37.0 4 36.595 37.0 37.0 37.0 37.0 37.0 5 36.6565 37.0 37.0 37.0 37.0 37.0 6 36.686 37.0 37.0 37.0 37.0 37.0 7 36.555 37.0 37.0 37.0 37.0 37.0 8 36.6385 37.0 37.0 37.0 37.0 37.0 9 36.571 37.0 37.0 37.0 37.0 37.0 10-14 36.61280000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.620799999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.603100000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.5367 37.0 37.0 37.0 37.0 37.0 30-34 36.548 37.0 37.0 37.0 37.0 37.0 35-39 36.522499999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.472899999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.470800000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.52168042010503 37.0 37.0 37.0 37.0 37.0 55-59 36.441460365091274 37.0 37.0 37.0 37.0 37.0 60-64 36.42975743935985 37.0 37.0 37.0 37.0 37.0 65-69 36.42235558889722 37.0 37.0 37.0 37.0 37.0 70-74 36.40414141004436 37.0 37.0 37.0 37.0 37.0 75-79 36.32712817975712 37.0 37.0 37.0 37.0 37.0 80-84 36.36858260092367 37.0 37.0 37.0 37.0 37.0 85-89 36.343885309468156 37.0 37.0 37.0 37.0 37.0 90-94 36.265330360872724 37.0 37.0 37.0 37.0 37.0 95-99 36.27345434841469 37.0 37.0 37.0 37.0 37.0 100-104 36.153374475308155 37.0 37.0 37.0 37.0 37.0 105-109 36.19434440542405 37.0 37.0 37.0 37.0 37.0 110-114 36.13001889688367 37.0 37.0 37.0 37.0 37.0 115-119 36.12564232349215 37.0 37.0 37.0 37.0 37.0 120-124 36.05985557935575 37.0 37.0 37.0 37.0 37.0 125-129 35.98757158479475 37.0 37.0 37.0 37.0 37.0 130-134 36.04227624504582 37.0 37.0 37.0 37.0 37.0 135-139 35.928344103198256 37.0 37.0 37.0 37.0 37.0 140-144 35.91462134733567 37.0 37.0 37.0 37.0 37.0 145-149 35.90360446966824 37.0 37.0 37.0 37.0 37.0 150 35.768400113668655 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 3.0 25 3.0 26 5.0 27 10.0 28 9.0 29 15.0 30 25.0 31 34.0 32 45.0 33 64.0 34 107.0 35 216.0 36 2911.0 37 550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.975 10.225 6.7 31.1 2 17.647058823529413 11.13892365456821 39.023779724655824 32.19023779724656 3 17.375 19.7 28.525 34.4 4 19.425 25.775 25.55 29.25 5 22.55 32.074999999999996 22.075 23.3 6 23.375 35.725 19.7 21.2 7 13.275 34.5 33.125 19.1 8 15.675 30.8 29.349999999999998 24.175 9 15.65 29.975 30.625000000000004 23.75 10-14 18.29 30.505 26.43 24.775 15-19 16.619999999999997 30.205 26.900000000000002 26.275 20-24 18.095 32.275 24.215 25.415 25-29 17.025000000000002 31.7 23.755000000000003 27.52 30-34 14.799999999999999 31.385 25.995 27.82 35-39 14.495 32.84 25.465 27.200000000000003 40-44 15.770000000000001 35.335 22.57 26.325 45-49 13.66 33.155 22.189999999999998 30.995 50-54 17.189297324331083 34.42360590147537 20.59014753688422 27.796949237309327 55-59 18.57464366091523 34.058514628657164 22.465616404101024 24.901225306326584 60-64 21.005251312828207 29.612403100775193 23.980995248812203 25.401350337584393 65-69 19.70492623155789 29.592398099524882 26.156539134783696 24.546136534133534 70-74 21.23774264558735 27.776665999599757 26.205723434060435 24.77986792075245 75-79 22.193852007609895 28.692299989986985 25.408030439571444 23.705817562831683 80-84 21.223958333333336 33.22315705128205 24.21374198717949 21.339142628205128 85-89 18.06154154555478 30.83592262203067 24.55647990377869 26.546055928635866 90-94 18.906171600602107 32.05218263923733 24.1294530858003 24.912192674360263 95-99 19.15866713574911 33.84932401869629 22.933105493290444 24.05890335226416 100-104 17.834973504920516 35.40247287408529 24.673227353015392 22.089326267978805 105-109 20.158882760714466 30.42048272023478 25.335222385265393 24.085412133785354 110-114 21.560637701828554 29.205928793358122 27.703356593490554 21.530076911322773 115-119 20.037029417815262 29.53096070767332 30.13783172186793 20.29417815264349 120-124 18.472459712938495 29.436758381263278 29.996372869060572 22.094409036737655 125-129 18.187536034383353 32.826668064363965 27.16075265999266 21.825043241260026 130-134 22.58904837852206 26.698564593301437 27.527910685805423 23.18447634237108 135-139 18.598733012074288 29.34105798906275 25.583410038442793 26.47679896042016 140-144 18.138174594415627 26.459551925836 27.43626531287937 27.966008166869 145-149 15.736782902137234 29.010123734533185 25.731158605174354 29.521934758155233 150 19.494174481386757 25.547030406365444 22.47797669792555 32.48081841432225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 1.0 17 1.5 18 1.0 19 1.0 20 1.0 21 1.0 22 0.5 23 0.5 24 2.0 25 2.5 26 3.5 27 9.0 28 14.5 29 20.0 30 21.0 31 20.0 32 36.0 33 58.0 34 82.5 35 132.5 36 198.0 37 213.0 38 149.5 39 121.5 40 120.0 41 143.0 42 241.5 43 245.5 44 177.5 45 138.0 46 117.0 47 97.0 48 122.0 49 165.0 50 181.0 51 247.0 52 308.0 53 206.5 54 99.5 55 118.5 56 83.5 57 22.0 58 6.5 59 11.5 60 14.0 61 16.0 62 11.5 63 2.0 64 1.5 65 3.5 66 3.5 67 1.5 68 0.0 69 0.0 70 4.0 71 4.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 45-49 1.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 1.0 70-74 2.0 75-79 2.0 80-84 1.0 85-89 5.0 90-94 5.0 95-99 12.0 100-104 13.0 105-109 17.0 110-114 42.0 115-119 23.0 120-124 47.0 125-129 42.0 130-134 60.0 135-139 83.0 140-144 59.0 145-149 66.0 150-151 3519.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.349999999999998 #Duplication Level Percentage of deduplicated Percentage of total 1 49.65928449744463 14.575 2 19.6763202725724 11.55 3 10.136286201022147 8.924999999999999 4 5.621805792163544 6.6000000000000005 5 3.9182282793867125 5.75 6 2.2146507666098807 3.9 7 2.1294718909710393 4.375 8 1.1073253833049403 2.6 9 0.7666098807495741 2.025 >10 4.173764906303236 24.9 >50 0.34071550255536626 5.800000000000001 >100 0.2555366269165247 9.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACC 133 3.325 No Hit GTTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCG 124 3.1 No Hit GTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATC 103 2.5749999999999997 No Hit GGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACC 66 1.6500000000000001 No Hit CTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAG 58 1.4500000000000002 No Hit GTCCTACACTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACC 54 1.35 No Hit CACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAGTGA 54 1.35 No Hit GCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAGTG 49 1.225 No Hit GGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACC 48 1.2 No Hit GCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCA 46 1.15 No Hit CCTAGCAATAAACCAATACACAAGTGCCCTTATGTAGACAAACCCAACAA 42 1.05 No Hit CTAGCAATAAACCAATACACAAGTGCCCTTATGTAGACAAACCCAACAAC 38 0.95 No Hit CACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCA 38 0.95 No Hit GTCCAGTCAGGTACCTTCCAGAGACAGTCTAAAAGAATTTCATAATTCTC 34 0.8500000000000001 No Hit AGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCAC 33 0.8250000000000001 No Hit GTGTTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAG 31 0.775 No Hit GCTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTC 28 0.7000000000000001 No Hit GTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGC 28 0.7000000000000001 No Hit GCATTACCAAAATCTCTGTTATGAGAACCTTCTGTCAAGGTAAATGGCGC 27 0.675 No Hit GCCTAGCAATAAACCAATACACAAGTGCCCTTATGTAGACAAACCCAACA 25 0.625 No Hit CCCCAGTTCATCAAACATTGCACAAATGACTGTTTCAACACTATCTACAG 23 0.575 No Hit CCTACACTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACCAA 22 0.5499999999999999 No Hit GGTGTTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACA 22 0.5499999999999999 No Hit GCGACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAATAAC 22 0.5499999999999999 No Hit GTCAAGGTAAATGGCGCCGCTGGATAGGTACTCGATTTCTAGTTTTCTAC 19 0.475 No Hit GTTCCATTAACAACTGGAATGTGCAGTTTGAAGGCTCAATAACCATAACA 19 0.475 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 19 0.475 No Hit CAATAAACCAATACACAAGTGCCCTTATGTAGACAAACCCAACAACATCC 18 0.44999999999999996 No Hit GAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCA 17 0.42500000000000004 No Hit CCGTACTCTTCGGTCATAACATGTTATGAAGCATCCCGATAACCCGAGTA 17 0.42500000000000004 No Hit CTCCGTACTCTTCGGTCATAACATGTTATGAAGCATCCCGATAACCCGAG 17 0.42500000000000004 No Hit TAGCAATAAACCAATACACAAGTGCCCTTATGTAGACAAACCCAACAACA 17 0.42500000000000004 No Hit GCCGTTTTCATGGTGCCTCAAGATCCATTCATACAGCTTTTCCATGGCGT 15 0.375 No Hit GTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCGACTAACTGA 15 0.375 No Hit GTTGCATTACCAAAATCTCTGTTATGAGAACCTTCTGTCAAGGTAAATGG 15 0.375 No Hit GCAGTTTGAAGGCTCAATAACCATAACACCGGAGTTGAAGAGTGTTGCAT 15 0.375 No Hit GTCATAACATGTTATGAAGCATCCCGATAACCCGAGTAAAGCAAAAAACA 14 0.35000000000000003 No Hit TGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAAC 14 0.35000000000000003 No Hit GCGGAAATCATTAATATCAAGGCATAAATGCATAATGCCTAGCAATAAAC 14 0.35000000000000003 No Hit CCTCAATACAGTCAACCACCACCAAAGCACCATCAGTGATACGAAGGGCG 14 0.35000000000000003 No Hit GCTACTTGTTGCATTACCAAAATCTCTGTTATGAGAACCTTCTGTCAAGG 14 0.35000000000000003 No Hit GCTGGATGTGCGAGGCTATATCAGCAACTGTCAGCCTCAAGCCGTTTTCA 13 0.325 No Hit GTGTTGTTTACCACTGAACTTAGCGGGTATTGCCTAAATCCAGCCATTGC 12 0.3 No Hit CTTGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCA 12 0.3 No Hit TCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCA 12 0.3 No Hit CTCGCATAGGGTATTCATTCAAGCTGATAAACCAAAACAAGTGCATTTTG 12 0.3 No Hit CTCTGTTATGAGAACCTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTA 12 0.3 No Hit GTGCTGAACAGGAAGCAGCCAGCTGCTCATGAGGCTGCTGCCATGGCCTA 11 0.27499999999999997 No Hit GTTATGAGAACCTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTACTCG 11 0.27499999999999997 No Hit GACCAGACAGCATTGACACTAGCCCTGTAGTAGACGGCCCTACACCAACA 11 0.27499999999999997 No Hit GCCTGCACATTTTTTGGTGTCAGAATAATACTATCACGCTGATCCATTAA 11 0.27499999999999997 No Hit CCCAGTTCATCAAACATTGCACAAATGACTGTTTCAACACTATCTACAGA 10 0.25 No Hit GTGCGGAACACCCTTCTTTTGGAGTTCATGTCCATGATGATGTTGCAAGG 10 0.25 No Hit GTGCAGTTTGAAGGCTCAATAACCATAACACCGGAGTTGAAGAGTGTTGC 10 0.25 No Hit ACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAGTGAT 10 0.25 No Hit AGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCAT 10 0.25 No Hit CCACTGAATAAAAGCATCAGACTGTTTAACAAAAACTTTATCAGCCTCGT 9 0.22499999999999998 No Hit GTGAACAGTAATTACTTGTAACACAGCGACTAACTGAACACTCACCTTGT 9 0.22499999999999998 No Hit GCTTCCTTAAGCCACTGAATAAAAGCATCAGACTGTTTAACAAAAACTTT 9 0.22499999999999998 No Hit CTGATCTTGAAGTTTAATCCTTGGATGCAGTCTTGGATATCTGACAGTTT 9 0.22499999999999998 No Hit GCCTTCCATTTTCCAAACACCTCATGCCTCTCAATCCTATCAGCACCCTC 9 0.22499999999999998 No Hit CCCTACACCAACATTGCCTTCCGCAGAAACATCCCTCCTTAGCTTTGTCC 9 0.22499999999999998 No Hit CTGCAGCTTTGCTTGTCGACAAGCTGGACCCATTTCTAACAGCACCATGC 9 0.22499999999999998 No Hit ACTCACCTTGTCTTTTAATCGAAACTTACAAATAACCAAAAAAGACATCT 9 0.22499999999999998 No Hit CCTCAGCTTCCTTAAGCCACTGAATAAAAGCATCAGACTGTTTAACAAAA 9 0.22499999999999998 No Hit CCAGAGACAGTCTAAAAGAATTTCATAATTCTCCACGCCTTTTCCGAAAT 8 0.2 No Hit GTTTGAAGGCTCAATAACCATAACACCGGAGTTGAAGAGTGTTGCATTGT 8 0.2 No Hit GGCAACATTATGTCTTGCTTAAACACCTTAGGTCAGACATAGCTTGGTGA 8 0.2 No Hit AGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAG 8 0.2 No Hit GTTCGGATGTATTTTCCCTTGGAAGAACTGTTGGTGGTGTACTAAATCTG 8 0.2 No Hit CTTCGGTCATAACATGTTATGAAGCATCCCGATAACCCGAGTAAAGCAAA 8 0.2 No Hit GTTTTCTGGTGAAAAGTTATTTGTGGCAGCTTCCATCTCAGAGAATTCGA 8 0.2 No Hit GTGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCA 8 0.2 No Hit GTCAGACATAGCTTGGTGATTAGTTTCTGATAACCAACACCAAGGCTCAA 8 0.2 No Hit CCCTTCTTTTGGAGTTCATGTCCATGATGATGTTGCAAGGAAGGATCATG 8 0.2 No Hit GCTCAATAACCATAACACCGGAGTTGAAGAGTGTTGCATTGTTTCCAGTT 8 0.2 No Hit CACCATTGTAAGATGTTATCTCATTGATATGTTCCATTAACAACTGGAAT 8 0.2 No Hit CCTTGCTCAGAAACTTCTGACCTGCAGCTTTGCTTGTCGACAAGCTGGAC 8 0.2 No Hit GGTCATAACATGTTATGAAGCATCCCGATAACCCGAGTAAAGCAAAAAAC 7 0.17500000000000002 No Hit GTACGGTATCACTGAGACAGCGCGCAAGTTGGCACCTGCAGTCTCTCCTC 7 0.17500000000000002 No Hit GTACTCTTCGGTCATAACATGTTATGAAGCATCCCGATAACCCGAGTAAA 7 0.17500000000000002 No Hit GATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACCAAAATCTCTGTT 7 0.17500000000000002 No Hit CCTGCAGCTTTGCTTGTCGACAAGCTGGACCCATTTCTAACAGCACCATG 7 0.17500000000000002 No Hit AGTCAGGTACCTTCCAGAGACAGTCTAAAAGAATTTCATAATTCTCCACG 7 0.17500000000000002 No Hit GCAGAAAATAACTAGAACAAGGTTTATATTAAGCAATATGCACAAAAGAA 7 0.17500000000000002 No Hit CTCTTCTTCGTCGTCGTCTTCCTCCTCAGCTTCCTTAAGCCACTGAATAA 7 0.17500000000000002 No Hit GCCAAGTCACTCCGTACTCTTCGGTCATAACATGTTATGAAGCATCCCGA 7 0.17500000000000002 No Hit CTCGGCTGGTGTAGCATTTTTATTCTTCTTGCCTCTCGGATCTCTTTTCT 7 0.17500000000000002 No Hit ATATTTGAATGCATAAGATACCGCCTAGAAAATTTGAAATTATGATTACT 7 0.17500000000000002 No Hit CGGAAATCATTAATATCAAGGCATAAATGCATAATGCCTAGCAATAAACC 7 0.17500000000000002 No Hit GGCAATAAACCAATACACAAGTGCCCTTATGTAGACAAACCCAACAACAT 7 0.17500000000000002 No Hit GGAAGAACTGTTGGTGGTGTACTAAATCTGGCTGGTATCATCATGAATCT 7 0.17500000000000002 No Hit CACTGAATAAAAGCATCAGACTGTTTAACAAAAACTTTATCAGCCTCGTC 7 0.17500000000000002 No Hit TCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACC 7 0.17500000000000002 No Hit AGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAAC 7 0.17500000000000002 No Hit CTTGCTCAGAAACTTCTGACCTGCAGCTTTGCTTGTCGACAAGCTGGACC 7 0.17500000000000002 No Hit AACTACTATGATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCCTC 7 0.17500000000000002 No Hit GGCTCAATAACCATAACACCGGAGTTGAAGAGTGTTGCATTGTTTCCAGT 7 0.17500000000000002 No Hit CCCTGTAGTAGACGGCCCTACACCAACATTGCCTTCCGCAGAAACATCCC 7 0.17500000000000002 No Hit CACTCACCTTGTCTTTTAATCGAAACTTACAAATAACCAAAAAAGACATC 7 0.17500000000000002 No Hit GTCGCGAGATGAGCATAGTATGCAGGAGGCACAATTGATACAGAACGAGT 7 0.17500000000000002 No Hit GGCCATAAATGTTTCAGAATCAGAAGCCTCATTTGGACTTGTATTTTCAT 7 0.17500000000000002 No Hit GTCAGCCTCAAGCCGTTTTCATGGTGCCTCAAGATCCATTCATACAGCTT 7 0.17500000000000002 No Hit GGTCTGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCAT 6 0.15 No Hit GTATCACTGAGACAGCGCGCAAGTTGGCACCTGCAGTCTCTCCTCAAAGG 6 0.15 No Hit GCTCCTAGAGTGGCAAGCTTTATGAAATTTTTTAATTAGTATATTTGAAT 6 0.15 No Hit GGCATAAGTAATGAAAAAACAAGATTTCCAGATGAAGCAATGGACCCAAG 6 0.15 No Hit TTTTTTTTGACGCAAGACGACACAGGTCTGTACATATTAGTACATCCGTA 6 0.15 No Hit GCACAAATGACTGTTTCAACACTATCTACAGAACGCAAATGAGAAACCTC 6 0.15 No Hit GGTATCACTGAGACAGCGCGCAAGTTGGCACCTGCAGTCTCTCCTCAAAG 6 0.15 No Hit CTGTAGTAGACGGCCCTACACCAACATTGCCTTCCGCAGAAACATCCCTC 6 0.15 No Hit GCTACAAATATATCACTCAGCGTAAAACACATATATCACTCAAAGAACAT 6 0.15 No Hit ATGAGAACCTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTACTCGATT 6 0.15 No Hit GTCAGTGAACAGTAATTACTTGTAACACAGCGACTAACTGAACACTCACC 6 0.15 No Hit CCAAGCTTTCAAATGGATGCAACTACTATGATCTCGAACTCTACTCTTCT 6 0.15 No Hit GTCGTTTCATTCCTCGATCAACAAGTGAACTCGTCCCACGACAAAACTTG 6 0.15 No Hit ATGATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCCTCAGCTTCC 6 0.15 No Hit GCTCCGTACTCTTCGGTCATAACATGTTATGAAGCATCCCGATAACCCGA 6 0.15 No Hit GGAAGAAGCAGCGGGCTACCATCTGGCAGGGAGGGCAGGCGCGGGCGACG 6 0.15 No Hit GGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCACCAC 6 0.15 No Hit GTCCATTCTCCGGTCACCTCTCCAGGTGGATGGAAAACTCTTGCCACTGG 6 0.15 No Hit GCTTCCACCATAAATTGATCGATGCATCTCTCATGGCCTGATCTTCGGCA 6 0.15 No Hit CTCATTGATATGTTCCATTAACAACTGGAATGTGCAGTTTGAAGGCTCAA 6 0.15 No Hit GTCCAACAGCCAGTTGGCAACATACCATCTTGTAGTTAAAAGACCTTCAA 6 0.15 No Hit CAGGGCTCTTCATTGCTAGTTGACTGCAAACAAAAGAAAAGAAAAAACAA 6 0.15 No Hit GGCACATGAAAACTGATAGCCAAGCTTTTCCATGTTCTATCTTTGTCCTA 6 0.15 No Hit CTACACTATGATTTGAGCTTTGAATGACAGCTACTTGTTGCATTACCAAA 6 0.15 No Hit CCCATTTCTAACAGCACCATGCATCCTTCCATTGTCAAGTGATGAAGCCC 6 0.15 No Hit CACAGCGACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAA 6 0.15 No Hit CTTCTGTCAAGGTAAATGGCGCCGCTGGATAGGTACTCGATTTCTAGTTT 5 0.125 No Hit GGATGCAACTACTATGATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTC 5 0.125 No Hit GGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCAC 5 0.125 No Hit CTTCATTTGTTCCTCTTCCTGCTCTTTCTTCAGTTTCAGAAGGTCAGCTT 5 0.125 No Hit GCTCAAGTACGGTATCACTGAGACAGCGCGCAAGTTGGCACCTGCAGTCT 5 0.125 No Hit CTGGTATCACTGAGACAGCGCGCAAGTTGGCACCTGCAGTCTCTCCTCAA 5 0.125 No Hit CCTACACCAACATTGCCTTCCGCAGAAACATCCCTCCTTAGCTTTGTCCC 5 0.125 No Hit CCCAGATACATCGTGGTTTTCGATCTGCAACTGGATAAATCCATCTGCAT 5 0.125 No Hit GCCTATAGTAGTTGTTGTAACTATCCAAAAGTGTTTTGATGGTGTTGTTT 5 0.125 No Hit GCCACTGAATAAAAGCATCAGACTGTTTAACAAAAACTTTATCAGCCTCG 5 0.125 No Hit CAAAAAATTTCCAGCCTGTCGGAACCTCAAAAAATGGAACATTTAATTTC 5 0.125 No Hit ATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCCTCAGCTTCCTTA 5 0.125 No Hit ACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCGACT 5 0.125 No Hit GTCGCTTCATTTGTTCCTCTTCCTGCTCTTTCTTCAGTTTCAGAAGGTCA 5 0.125 No Hit CGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCAC 5 0.125 No Hit AGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAACCAC 5 0.125 No Hit TGCACACAGACACCCTCAATACAGTCAACCACCACCAAAGCACCATCAGT 5 0.125 No Hit GACAGCTACTTGTTGCATTACCAAAATCTCTGTTATGAGAACCTTCTGTC 5 0.125 No Hit CATGCTGGATGTGCGAGGCTATATCAGCAACTGTCAGCCTCAAGCCGTTT 5 0.125 No Hit AGTTCATCAAACATTGCACAAATGACTGTTTCAACACTATCTACAGAACG 5 0.125 No Hit CAGCGACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAATA 5 0.125 No Hit TCCTGGTGTGTGCTGAACAGGAAGCAGCCAGCTGCTCATGAGGCTGCTGC 5 0.125 No Hit CCCTTGCTCAGAAACTTCTGACCTGCAGCTTTGCTTGTCGACAAGCTGGA 5 0.125 No Hit GTGCTGACGCCTCGATCCTTCAGGGCAGAAGAGTGCCATGAGAGGAAATT 5 0.125 No Hit GACTAACTGAACACTCACCTTGTCTTTTAATCGAAACTTACAAATAACCA 5 0.125 No Hit CTCTCTCATCTATTGTGCCCGGAACAGCAACATTGATTAACTTGCAAAGT 5 0.125 No Hit GCTCTTCGGAACGCGAACATTTCTTCTTGACCAGACAGCATTGACACTAG 5 0.125 No Hit CCTTAGCTTTGTCCCTTCGGCTTCAGTTTCAAACTGGAAACTCTCTAAAT 5 0.125 No Hit CTCCATTAAATTTGAAAAAGTTTGACTCCGAAGAGAGTTAGGTCTCCTTA 5 0.125 No Hit GCCACAGCCCGCACACGATGAATCAGCAAATCACAAATCAACAATTTAGT 5 0.125 No Hit CTTAGCTTTGTCCCTTCGGCTTCAGTTTCAAACTGGAAACTCTCTAAATC 5 0.125 No Hit ATATTTGTCAAGCTGTACCGAATAGGCGAAGAACACAAAACGAGCTGACG 5 0.125 No Hit CTTTGCTTGTCGACAAGCTGGACCCATTTCTAACAGCACCATGCATCCTT 5 0.125 No Hit CTTGAGAATAGAAATCACTTTCAGAAGTGAATGGGAGCCGGTTGTTCCAG 5 0.125 No Hit GTCAGTGTTCTCGGAATTGGGGTAGCCGACATAGCAAGAACATGTGGAGC 5 0.125 No Hit TGCAGTTTGAAGGCTCAATAACCATAACACCGGAGTTGAAGAGTGTTGCA 5 0.125 No Hit GGGCGGAGCACAGTTTCAGTCTGCACACAGACACCCTCAATACAGTCAAC 5 0.125 No Hit GTCGGACATAGCTTGGTGATTAGTTTCTGATAACCAACACCAAGGCTCAA 5 0.125 No Hit GTTTTGATGGTGTTGTTTACCACTGAACTTAGCGGGTATTGCCTAAATCC 5 0.125 No Hit TACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCGAC 5 0.125 No Hit GTGAAAAGTTATTTGTGGCAGCTTCCATCTCAGAGAATTCGAACCTTTTT 5 0.125 No Hit CTCAATACAGTCAACCACCACCAAAGCACCATCAGTGATACGAAGGGCGG 5 0.125 No Hit CAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCGACTAACTGAACAC 5 0.125 No Hit GCCCTGTAGTAGACGGCCCTACACCAACATTGCCTTCCGCAGAAACATCC 5 0.125 No Hit GCAACTACTATGATCTCGAACTCTACTCTTCTTCGTCGTCGTCTTCCTCC 5 0.125 No Hit CTCGCCTTCCATTTTCCAAACACCTCATGCCTCTCAATCCTATCAGCACC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCATAA 10 0.0070813857 143.24051 1 CTACACT 10 0.0073563093 141.45 4 TACACTA 10 0.0073563093 141.45 5 ACTATGA 10 0.0073563093 141.45 8 GTTACAA 20 3.762658E-4 107.43038 1 ACAAAGG 20 3.9595735E-4 106.087494 4 GGTCACA 20 3.9595735E-4 106.087494 9 TACAAAG 20 3.9595735E-4 106.087494 3 AAAGGTC 20 3.9595735E-4 106.087494 6 AAGGTCA 20 3.9595735E-4 106.087494 7 CAAAGGT 20 3.9595735E-4 106.087494 5 AGGTCAC 20 3.9595735E-4 106.087494 8 GCACAGT 45 0.009391226 47.746838 1 GTCACAA 20 0.006699163 28.289999 10-14 TCACAAG 20 0.006699163 28.289999 10-14 TAGATTT 40 0.0076249205 18.134615 110-114 TACTTGT 55 0.002968523 15.430909 35-39 AGTCTGC 65 0.008997737 13.056923 10-14 >>END_MODULE ERR5262808 read2 length is 49-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262808_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 49-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1475 37.0 37.0 37.0 37.0 37.0 2 35.906 37.0 37.0 37.0 37.0 37.0 3 35.9765 37.0 37.0 37.0 37.0 37.0 4 36.0335 37.0 37.0 37.0 37.0 37.0 5 36.146 37.0 37.0 37.0 37.0 37.0 6 36.1195 37.0 37.0 37.0 37.0 37.0 7 36.2155 37.0 37.0 37.0 37.0 37.0 8 36.1755 37.0 37.0 37.0 37.0 37.0 9 36.203 37.0 37.0 37.0 37.0 37.0 10-14 36.2149 37.0 37.0 37.0 37.0 37.0 15-19 36.2278 37.0 37.0 37.0 37.0 37.0 20-24 36.1464 37.0 37.0 37.0 37.0 37.0 25-29 36.1417 37.0 37.0 37.0 37.0 37.0 30-34 36.102500000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.055899999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.1201 37.0 37.0 37.0 37.0 37.0 45-49 36.05459999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.02475618904727 37.0 37.0 37.0 37.0 37.0 55-59 36.014053513378336 37.0 37.0 37.0 37.0 37.0 60-64 35.932733183295824 37.0 37.0 37.0 37.0 37.0 65-69 35.94873718429608 37.0 37.0 37.0 37.0 37.0 70-74 35.903054680493405 37.0 37.0 37.0 37.0 37.0 75-79 35.880732259550484 37.0 37.0 37.0 37.0 37.0 80-84 35.81930478989208 37.0 37.0 37.0 37.0 37.0 85-89 35.8527621625234 37.0 37.0 37.0 37.0 37.0 90-94 35.75205836294797 37.0 37.0 37.0 37.0 37.0 95-99 35.78240902942029 37.0 37.0 37.0 37.0 37.0 100-104 35.73248359704487 37.0 37.0 37.0 37.0 37.0 105-109 35.69370165246091 37.0 37.0 37.0 37.0 37.0 110-114 35.64956249333752 37.0 37.0 37.0 37.0 37.0 115-119 35.71708609335437 37.0 37.0 37.0 37.0 37.0 120-124 35.66663261807649 37.0 37.0 37.0 37.0 37.0 125-129 35.55855860218295 37.0 37.0 37.0 37.0 37.0 130-134 35.501743726205135 37.0 37.0 37.0 37.0 37.0 135-139 35.48982617771268 37.0 37.0 37.0 37.0 37.0 140-144 35.4660396056906 37.0 37.0 37.0 37.0 37.0 145-149 35.477317502061695 37.0 37.0 37.0 34.6 37.0 150 35.328681287382516 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 2.0 13 1.0 14 1.0 15 0.0 16 0.0 17 1.0 18 0.0 19 1.0 20 0.0 21 5.0 22 7.0 23 3.0 24 5.0 25 11.0 26 9.0 27 12.0 28 17.0 29 10.0 30 25.0 31 43.0 32 54.0 33 101.0 34 195.0 35 588.0 36 2664.0 37 241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.150000000000006 22.650000000000002 9.0 22.2 2 29.349999999999998 23.724999999999998 30.125 16.8 3 22.825 24.825 32.75 19.6 4 23.525 31.3 25.474999999999998 19.7 5 28.000000000000004 32.7 21.25 18.05 6 22.95 36.525 20.575 19.950000000000003 7 24.125 21.25 34.975 19.650000000000002 8 24.275 25.174999999999997 25.900000000000002 24.65 9 24.6 25.0 28.775000000000002 21.625 10-14 26.13 26.77 25.61 21.490000000000002 15-19 25.06 27.775 25.905 21.26 20-24 25.180000000000003 27.800000000000004 25.7 21.32 25-29 25.75 27.38 26.064999999999998 20.805 30-34 25.825 26.44 26.265 21.47 35-39 25.724999999999998 28.494999999999997 26.419999999999998 19.36 40-44 26.284999999999997 26.215 26.619999999999997 20.880000000000003 45-49 23.915 27.83 26.41 21.845 50-54 24.141035258814703 27.09677419354839 27.246811702925733 21.51537884471118 55-59 24.45111277819455 26.541635408852216 28.092023005751436 20.9152288072018 60-64 23.995998999749936 26.046511627906977 29.62240560140035 20.335083770942735 65-69 24.90622655663916 24.681170292573142 28.127031757939484 22.28557139284821 70-74 24.359615769461676 24.5347208324995 29.38262957774665 21.723033820292176 75-79 23.99619505356964 25.658355862621406 28.892560328426953 21.452888755381995 80-84 24.544270833333336 25.55088141025641 29.542267628205128 20.362580128205128 85-89 24.676756540042096 26.18021449333467 27.89916808659918 21.243860880024055 90-94 26.47265429001505 25.619668840943298 27.651781234320122 20.255895634721526 95-99 26.692466200934817 24.295119867316682 29.71302206362768 19.29939186812082 100-104 27.141054756497603 23.981831945495838 29.01337370678779 19.863739591218774 105-109 24.62176795020999 25.4667813591054 28.482517836360877 21.428932854323737 110-114 25.818774512300717 26.047980441094076 28.227983497173128 19.90526154943208 115-119 25.361312554646915 26.05564984827444 29.218741963688732 19.364295633389908 120-124 25.011661052086033 25.581756931847625 29.012697590049235 20.393884426017102 125-129 24.98820569271898 26.974891230277297 28.41117576138806 19.625727315615663 130-134 24.92022973835354 25.1914486279515 29.919166134864923 19.969155498830034 135-139 26.14683873766403 26.504717492679752 28.424248996855006 18.924194772801215 140-144 24.946093879582023 27.01940620335047 29.291756510200695 18.74274340686681 145-149 24.91967758299983 27.033425398793753 28.442590609323037 19.604306408883375 150 23.29820563941897 28.16861293078895 27.741384221019654 20.79179720877243 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 1.0 22 1.0 23 0.0 24 2.0 25 3.5 26 3.0 27 7.5 28 9.0 29 6.0 30 8.0 31 14.5 32 23.0 33 26.5 34 29.5 35 41.5 36 71.5 37 89.0 38 89.5 39 99.0 40 124.5 41 170.5 42 176.5 43 174.0 44 229.5 45 276.0 46 314.0 47 367.5 48 339.0 49 261.5 50 208.5 51 149.0 52 112.0 53 101.5 54 73.0 55 42.5 56 36.0 57 26.5 58 21.0 59 32.0 60 28.5 61 17.5 62 16.0 63 17.5 64 18.5 65 22.0 66 24.0 67 14.0 68 12.5 69 19.5 70 13.0 71 7.0 72 7.5 73 5.0 74 7.5 75 7.0 76 2.0 77 1.5 78 1.0 79 0.5 80 0.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 45-49 1.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 1.0 70-74 2.0 75-79 2.0 80-84 1.0 85-89 5.0 90-94 5.0 95-99 12.0 100-104 13.0 105-109 17.0 110-114 42.0 115-119 24.0 120-124 46.0 125-129 43.0 130-134 64.0 135-139 85.0 140-144 59.0 145-149 67.0 150-151 3511.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.1 #Duplication Level Percentage of deduplicated Percentage of total 1 54.4468546637744 25.1 2 20.98698481561822 19.35 3 10.629067245119305 14.7 4 5.206073752711497 9.6 5 3.14533622559653 7.249999999999999 6 1.735357917570499 4.8 7 1.2472885032537961 4.025 8 0.32537960954446854 1.2 9 0.596529284164859 2.475 >10 1.6811279826464207 11.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCT 24 0.6 No Hit GGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGA 22 0.5499999999999999 No Hit GAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAA 21 0.525 No Hit AGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTTCTACG 21 0.525 No Hit GGGAGGTGGAAGCGAGACCCCTAGGAACTTCTCTTTGTTGCCTGATATTC 20 0.5 No Hit ATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGT 20 0.5 No Hit GGCAAATCTACGCTTACGGATTCCCTTGTGGCAGCTGCTGGGATTATTGC 19 0.475 No Hit TGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACC 18 0.44999999999999996 No Hit CTCATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGACC 17 0.42500000000000004 No Hit CACAAATCTGTTGCGGACAACACCTGCTGCAGTTATAACCTTCACAAGCT 16 0.4 No Hit GGACAAGAAGAACAACATCCGCAACATGTCTGTTATTGCTCATGTGGACC 16 0.4 No Hit GTTGCTGGTGATGTTCGCATGACTGATACCCGTGCAGATGAAGCAGAGCG 16 0.4 No Hit AGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACCTGATC 16 0.4 No Hit ATCTCATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGA 15 0.375 No Hit GGGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTG 15 0.375 No Hit GTTCGCTCTTCGCGGAGACCACCGGCGAACCGTAGTCCCCAGCCATAGCC 14 0.35000000000000003 No Hit GATGAAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTT 13 0.325 No Hit CTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAG 13 0.325 No Hit GCTTCCTCATCTCATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGG 12 0.3 No Hit GTTCGCATGACTGATACCCGTGCAGATGAAGCAGAGCGTGGTATTACGAT 12 0.3 No Hit GCTGGGATTATTGCCCAGGAAGTTGCTGGTGATGTTCGCATGACTGATAC 12 0.3 No Hit AGATGATTCACAGATTCCTTCTCGATCTATGCCCTCCAGAATCTGAACAG 12 0.3 No Hit GGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTT 11 0.27499999999999997 No Hit GGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGG 11 0.27499999999999997 No Hit AATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGATTTTTC 11 0.27499999999999997 No Hit GGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGATTT 11 0.27499999999999997 No Hit CTCTCTTTTCTACGAGATGACTGATGAGTCACTCCAGATGTACAAGGGTG 11 0.27499999999999997 No Hit GGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAA 11 0.27499999999999997 No Hit GGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATT 10 0.25 No Hit GAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACCTGAT 10 0.25 No Hit ATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGACCCCT 10 0.25 No Hit CTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAA 9 0.22499999999999998 No Hit GTCCAGGGTGGACGGGGTTGATCGTCTTGAAGGTCTGGTCTGTGAAGCCC 9 0.22499999999999998 No Hit GGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGATTTT 9 0.22499999999999998 No Hit GTGAAGTTTACAGCAGAAGAGCTTCGCCGGATTATGGACAAGAAGAACAA 9 0.22499999999999998 No Hit GGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGC 9 0.22499999999999998 No Hit GTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCC 9 0.22499999999999998 No Hit CTTCTCTTTGTTGCCTGATATTCAGAAACAGTCAAGATGGTGAAGTTTAC 9 0.22499999999999998 No Hit AGACATCTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAG 9 0.22499999999999998 No Hit GCCACAAATCTGTTGCGGACAACACCTGCTGCAGTTATAACCTTCACAAG 9 0.22499999999999998 No Hit ATTCTACAATGCGAGTACAAGATTCTCCGATGGGGCTCGTTTTGGACTCG 9 0.22499999999999998 No Hit GGTTGATCGTCTTGAAGGTCTGGTCTGTGAAGCCCGTAAGGTGCAGTGAT 9 0.22499999999999998 No Hit GGACAAGGCCGCTGCCGGCGAGCACAAGACCACCGCTTAATCGGAGCCGT 8 0.2 No Hit CTTATTGATTCACCTGGACACGTTGATTTTTCTTCGGAGGTCACAGCCGC 8 0.2 No Hit ATTCCCTTGTGGCAGCTGCTGGGATTATTGCCCAGGAAGTTGCTGGTGAT 8 0.2 No Hit CTTGTGGCAGCTGCTGGGATTATTGCCCAGGAAGTTGCTGGTGATGTTCG 8 0.2 No Hit ATTCATCTTTGCTCTTGTCCAGTTTGTCATCTCTGCATTGGCCTATTTGG 8 0.2 No Hit ACTCGCCTAGGGTTCGGGCCACCGATTCGGACCGAATTAGACGGCGGCGC 8 0.2 No Hit ATTACGATCAAATCCACTGGTATCTCTCTTTTCTACGAGATGACTGATGA 7 0.17500000000000002 No Hit ATGAAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTTC 7 0.17500000000000002 No Hit GGCAGCTTACAGATTATGACAAGATCATATTCATAGATGCCGATCTCCTC 7 0.17500000000000002 No Hit CCTCATCTCATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGC 7 0.17500000000000002 No Hit ATTCAGTTGCATTGCGATGGTATAAGCTTTGCTGCCATCCAATAAAAGGT 7 0.17500000000000002 No Hit TAGCCACCTTGATTTTTCTAACCGCTTAGCTGCGCGTACAAAGCTAGTTC 7 0.17500000000000002 No Hit GTCTGGATCGGGACTTGGGACCGGAACTTGATCGAAGTGTTTCGCTGTGT 7 0.17500000000000002 No Hit CGTCAAGGAGGGGTTCGCATTTTCTCATTGCACCACCTGCAAGGCCCCTT 7 0.17500000000000002 No Hit GTCCAGTTTGTCATCTCTGCATTGGCCTATTTGGTACATTTTATTGATGG 7 0.17500000000000002 No Hit TGAAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTTCT 7 0.17500000000000002 No Hit TCCCTTGTGGCAGCTGCTGGGATTATTGCCCAGGAAGTTGCTGGTGATGT 7 0.17500000000000002 No Hit GGAGACCATCCTTGGCCTCAAAAAGATGTTCACCTGGCGCTTCCAAAGGA 7 0.17500000000000002 No Hit CTCTGAAGCAAGATATCGAGGGGTAATAGACTGTGTTAGAAAGGTTTATC 7 0.17500000000000002 No Hit TAGACATCTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAA 7 0.17500000000000002 No Hit CATTCCTCTCAACCCTCTCGGATTGGGAGGTGGAAGCGAGACCCCTAGGA 7 0.17500000000000002 No Hit CTCACATTGCTTTTTGCTCTGTTGTTTGGGCCTTGGTGCCTTTGAGGAGA 7 0.17500000000000002 No Hit GGTTTATCATGGAGAGGGTATAGCTGGCTTCTACCGTGGGTGTGCCACAA 7 0.17500000000000002 No Hit GTTTCGCTGTGTTGTGTTCTGTTTTGCGTTTTATGTCCAGGGTGGACGGG 7 0.17500000000000002 No Hit CAGAAGAGCTTCGCCGGATTATGGACAAGAAGAACAACATCCGCAACATG 7 0.17500000000000002 No Hit CCTTCACAAGCTTTGAGATGATTCACAGATTCCTTCTCGATCTATGCCCT 7 0.17500000000000002 No Hit GTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGA 7 0.17500000000000002 No Hit GTTTAGCTGTCTCATCCATTGATGATCTTTTTCAAGGAACCGAGGAAGGA 7 0.17500000000000002 No Hit CTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGACCCCTAG 7 0.17500000000000002 No Hit CTTTTACGCAATTACACGAAGACAGTTGATGAGGAGATGGAAATACTGTT 6 0.15 No Hit GTGATTTTCGAGGATAAGGATGTAGACTTTTTTTCGCTGGAGGTCAAGAC 6 0.15 No Hit AGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATTGTT 6 0.15 No Hit GACAAGAAGAACAACATCCGCAACATGTCTGTTATTGCTCATGTGGACCA 6 0.15 No Hit GGTGATGTTCGCATGACTGATACCCGTGCAGATGAAGCAGAGCGTGGTAT 6 0.15 No Hit GAAGAGGAGTACGGGCACAACGAGGAGATGGCGCGGCTGGAAGCGTACAG 6 0.15 No Hit TCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGACCCCTA 6 0.15 No Hit GCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCTTTTCTACGA 6 0.15 No Hit GCTTTTGGGAGTTCTTGCATCCGTACTAGTAAGGAAAAAATGTGTGTTGT 6 0.15 No Hit GTCAAGATGGTGAAGTTTACAGCAGAAGAGCTTCGCCGGATTATGGACAA 6 0.15 No Hit GCCCAGGAAGTTGCTGGTGATGTTCGCATGACTGATACCCGTGCAGATGA 6 0.15 No Hit ATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGACCCCTAGGAA 6 0.15 No Hit ATCTAATCAGGGTCATGAAACAGGAGATAACAATAACTCAATTAGTGCAG 6 0.15 No Hit GCTAGCGAAGCGCATACCATCACTCTTGCCCTTGAAGGTCTCTTGGGTGT 6 0.15 No Hit GAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGATTTTTCTTC 6 0.15 No Hit GTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACCTGATCA 6 0.15 No Hit GCAACATGTCTGTTATTGCTCATGTGGACCATGGCAAATCTACGCTTACG 6 0.15 No Hit GATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAATACCT 6 0.15 No Hit TCTAAAGACAACTGCAGATGCACTTGCCAAGTGGAAGGACCTTTTACGCA 6 0.15 No Hit GATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACACGTTGA 6 0.15 No Hit GGATCGGGAGGTGGAAGCGAGACCCCTAGGAACTTCTCTTTGTTGCCTGA 6 0.15 No Hit CTGAACAGCATCCCCAGCCACTGAAGCATTGACTGTATCGTTACTGTGTC 6 0.15 No Hit CTGATACCCGTGCAGATGAAGCAGAGCGTGGTATTACGATCAAATCCACT 6 0.15 No Hit AGAAGAACAACATCCGCAACATGTCTGTTATTGCTCATGTGGACCATGGC 6 0.15 No Hit AGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCAT 6 0.15 No Hit AGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAA 6 0.15 No Hit GTTTACAGCAGAAGAGCTTCGCCGGATTATGGACAAGAAGAACAACATCC 6 0.15 No Hit CGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCC 6 0.15 No Hit TCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCA 6 0.15 No Hit GTTTGTGCCGCAGGTTGGTTCTGCTGTTCCTAATAGTTTGGAAAAAAATC 6 0.15 No Hit GCAGGGCTGAAGGTCGCTGTTGCATCTAGCGCCGATAGGATTAAGGTGGA 6 0.15 No Hit CCGGAACTTGATCGAAGTGTTTCGCTGTGTTGTGTTCTGTTTTGCGTTTT 6 0.15 No Hit CTGTAGCCTATATGTTGTTACGAAGATACTTAAGTTAAATCAATGGATGG 5 0.125 No Hit GGCCGAGGCACTTAAGGATAATCGGGACTATTCGTTTCTACTCTCAGATG 5 0.125 No Hit GCCATGCCCAAATAGAAATATCCTCTGACCATAGTTCGCTCTTCGCGGAG 5 0.125 No Hit CTACCGTGGGTGTGCCACAAATCTGTTGCGGACAACACCTGCTGCAGTTA 5 0.125 No Hit CGGGGTTGATCGTCTTGAAGGTCTGGTCTGTGAAGCCCGTAAGGTGCAGT 5 0.125 No Hit GGACGAGGAGTCACAGATCGTCCGGGAGGTCCTCCGCATCAAGGAGCTTC 5 0.125 No Hit GATTGTTGATCGCCTAGTCCCTGTGGAGAACACGACAACCCGCAGTTTGG 5 0.125 No Hit AGTTGAGTCAGGTAAACCAGGAAATCAAGGAGCACATGAACATTGCAAAC 5 0.125 No Hit GGAGGACAAGGCCGCTGCCGGCGAGCACAAGACCACCGCTTAATCGGAGC 5 0.125 No Hit CATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGACCCC 5 0.125 No Hit CTGAATAGTTCCCACTTTGATTTGTTTTGCTTGGCGTGGTGTCGCTGCCG 5 0.125 No Hit ATGGACAAGAAGAACAACATCCGCAACATGTCTGTTATTGCTCATGTGGA 5 0.125 No Hit GTGGGATGATATTTCCCATGGTGTGATTTTCGAGGATAAGGATGTAGACT 5 0.125 No Hit CAGAATAATTGAGAGGATCCGAAACCCAAAGGCTGAGCGTGATGCTTACA 5 0.125 No Hit GTGGACCATGGCAAATCTACGCTTACGGATTCCCTTGTGGCAGCTGCTGG 5 0.125 No Hit CTCTTTGTTGCCTGATATTCAGAAACAGTCAAGATGGTGAAGTTTACAGC 5 0.125 No Hit CATCTCATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAG 5 0.125 No Hit GTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGA 5 0.125 No Hit GTAACAAGCTAGGGGATGATCGAGAAGCTATAGCTACGAAGTGAGAAAAG 5 0.125 No Hit ATGACTGATACCCGTGCAGATGAAGCAGAGCGTGGTATTACGATCAAATC 5 0.125 No Hit AACATGTCTGTTATTGCTCATGTGGACCATGGCAAATCTACGCTTACGGA 5 0.125 No Hit TATCAAACCGCTCAGCAAAATAATGGCGAATCTTCCAATGAATGGCAGCT 5 0.125 No Hit GGTCAGAATAATTGAGAGGATCCGAAACCCAAAGGCTGAGCGTGATGCTT 5 0.125 No Hit GATCGGGAGGTGGAAGCGAGACCCCTAGGAACTTCTCTTTGTTGCCTGAT 5 0.125 No Hit GAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCACCTGGACA 5 0.125 No Hit GCCCTTTGAGTTCAATGCTATTCCAGCTGCTAGCCATGAGGTCCACCTCG 5 0.125 No Hit ACAAGGGTGAGAGGGATGGGAATGAATACCTGATCAACCTTATTGATTCA 5 0.125 No Hit GGAAATACTGTTGAAGTTTGAGGAAATGTGTCAAGAGACCACAAAAGAAT 5 0.125 No Hit GACTGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATGAAT 5 0.125 No Hit CTTCGCCGGATTATGGACAAGAAGAACAACATCCGCAACATGTCTGTTAT 5 0.125 No Hit CTTCTAGTAACAAGCTAGGGGATGATCGAGAAGCTATAGCTACGAAGTGA 5 0.125 No Hit TGATATTCAGAAACAGTCAAGATGGTGAAGTTTACAGCAGAAGAGCTTCG 5 0.125 No Hit CCTCTCGGATCGGGAGGTGGAAGCGAGACCCCTAGGAACTTCTCTTTGTT 5 0.125 No Hit AAGAAGAACAACATCCGCAACATGTCTGTTATTGCTCATGTGGACCATGG 5 0.125 No Hit GATTCCCTTGTGGCAGCTGCTGGGATTATTGCCCAGGAAGTTGCTGGTGA 5 0.125 No Hit CCGATACGAGCCAAACAATTGAATTTGAGACGTTCCTCAGGGAGTACTTA 5 0.125 No Hit GGCCGCTGCCGGCGAGCACAAGACCACCGCTTAATCGGAGCCGTAGTAGT 5 0.125 No Hit GTGGAAGGTCAGAATAATTGAGAGGATCCGAAACCCAAAGGCTGAGCGTG 5 0.125 No Hit GATGACTGATGAGTCACTCCAGATGTACAAGGGTGAGAGGGATGGGAATG 5 0.125 No Hit TCTCATCTCATTCCTCTCAACCCTCTCGGATCGGGAGGTGGAAGCGAGAC 5 0.125 No Hit CCGTGGGTGTGCCACAAATCTGTTGCGGACAACACCTGCTGCAGTTATAA 5 0.125 No Hit GGACCTTTTACGCAATTACACGAAGACAGTTGATGAGGAGATGGAAATAC 5 0.125 No Hit GAGCCCCAAAAAAGTTGACAGAGCTTCCATTGTTGTTCTCCTGCTCATCG 5 0.125 No Hit GGTTCATCACCCTAATTGATGAGATGTACAATCATCACTGCCGGCTTATA 5 0.125 No Hit GTGCCACAAATCTGTTGCGGACAACACCTGCTGCAGTTATAACCTTCACA 5 0.125 No Hit GCGAGAAGGGCATCAACCAGGATTGCTCCGTCGTCTGGGAGGTGAGCTAG 5 0.125 No Hit GTCCTATCCATGCTGTCATCCGAGAGTAGAAAACTAGAAATCGAGTACCT 5 0.125 No Hit AAAGGCAACACCTGCCGCTAGGTCTGCCTTGACTCAAAAGTCTGATCGCC 5 0.125 No Hit GGAAATGTGTCAAGAGACCACAAAAGAATTTTGTTCACTGTTTTCAAAGA 5 0.125 No Hit TAAAGGAGAAGCTTAGACTTCCTGTTGGTTCCTGTGAGCTTGCAGTTCCA 5 0.125 No Hit ACAGATTCCTTCTCGATCTATGCCCTCCAGAATCTGAACAGCATCCCCAG 5 0.125 No Hit GCTTAATCGGAGCCGTAGTAGTCTGGATCGGGACTTGGGACCGGAACTTG 5 0.125 No Hit GGGGACGTGGTTTGAGGAGAGGGGATGACGGGGAAGAAGCGGAAGGCGGA 5 0.125 No Hit AGAGCTTCGCCGGATTATGGACAAGAAGAACAACATCCGCAACATGTCTG 5 0.125 No Hit GATATTTCCCATGGTGTGATTTTCGAGGATAAGGATGTAGACTTTTTTTC 5 0.125 No Hit GCAGATGAAGCAGAGCGTGGTATTACGATCAAATCCACTGGTATCTCTCT 5 0.125 No Hit CCTGCTCATCGTGCTTTCGGTTTGTGCCGCAGGTTGGTTCTGCTGTTCCT 5 0.125 No Hit CTCTTTTCTACGAGATGACTGATGAGTCACTCCAGATGTACAAGGGTGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCTTT 10 0.0073621436 141.41249 4 ACATCTT 10 0.0073621436 141.41249 3 GACATCT 10 0.0073621436 141.41249 2 AGACATC 10 0.0073621436 141.41249 1 >>END_MODULE Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489471 spots for ERR5262808.sra Written 1489471 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra Read 1489467 spots for ERR5262808.sra Written 1489467 spots for ERR5262808.sra SRR ids: ['ERR5262808.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_fv6n9oqa ERR5262808.sra spots: 29789344 blocks: [[1, 1489467], [1489468, 2978934], [2978935, 4468401], [4468402, 5957868], [5957869, 7447335], [7447336, 8936802], [8936803, 10426269], [10426270, 11915736], [11915737, 13405203], [13405204, 14894670], [14894671, 16384137], [16384138, 17873604], [17873605, 19363071], [19363072, 20852538], [20852539, 22342005], [22342006, 23831472], [23831473, 25320939], [25320940, 26810406], [26810407, 28299873], [28299874, 29789344]] ERR5262808 file size 9781952 ERR5262808 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262808 ERR5262808_1.fastq ERR5262808_2.fastq Input file: ERR5262808_1.fastq Paired file: ERR5262808_2.fastq trimmed: ERR5262808-trimmed-pair1.fastq, ERR5262808-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:17:39 2024 >> started Fri Dec 6 12:18:20 2024 >> done (40.876s) 29789344 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 29789344 (100.00%) read pairs available; of these: 8615 ( 0.03%) trimmed read pairs available after processing 29780729 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 1 0.00% 28 0 0.00% 29 2 0.00% 30 1 0.00% 31 3 0.00% 32 2 0.00% 33 3 0.00% 34 3 0.00% 35 4 0.00% 36 1 0.00% 37 1 0.00% 38 0 0.00% 39 2 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 1 0.00% 44 1 0.00% 45 1 0.00% 46 6 0.00% 47 2 0.00% 48 1 0.00% 49 281 0.00% 50 284 0.00% 51 375 0.00% 52 405 0.00% 53 464 0.00% 54 445 0.00% 55 466 0.00% 56 622 0.00% 57 671 0.00% 58 775 0.00% 59 931 0.00% 60 1046 0.00% 61 1232 0.00% 62 1365 0.00% 63 1514 0.01% 64 1559 0.01% 65 1632 0.01% 66 2041 0.01% 67 2107 0.01% 68 2462 0.01% 69 2667 0.01% 70 3329 0.01% 71 3755 0.01% 72 4364 0.01% 73 4821 0.02% 74 5345 0.02% 75 6024 0.02% 76 6462 0.02% 77 6954 0.02% 78 7673 0.03% 79 8463 0.03% 80 9410 0.03% 81 11094 0.04% 82 12228 0.04% 83 13356 0.04% 84 14844 0.05% 85 16195 0.05% 86 17115 0.06% 87 17858 0.06% 88 19276 0.06% 89 20273 0.07% 90 22100 0.07% 91 23873 0.08% 92 25332 0.09% 93 27655 0.09% 94 29687 0.10% 95 31358 0.11% 96 33014 0.11% 97 34504 0.12% 98 35178 0.12% 99 36725 0.12% 100 38895 0.13% 101 39968 0.13% 102 42765 0.14% 103 45144 0.15% 104 46727 0.16% 105 49221 0.17% 106 50540 0.17% 107 51604 0.17% 108 53178 0.18% 109 54897 0.18% 110 56939 0.19% 111 58027 0.19% 112 61098 0.21% 113 62922 0.21% 114 65756 0.22% 115 67569 0.23% 116 69679 0.23% 117 71048 0.24% 118 72244 0.24% 119 72058 0.24% 120 73334 0.25% 121 75161 0.25% 122 76872 0.26% 123 79212 0.27% 124 82880 0.28% 125 83220 0.28% 126 85522 0.29% 127 86494 0.29% 128 87726 0.29% 129 88918 0.30% 130 89465 0.30% 131 90327 0.30% 132 92429 0.31% 133 94818 0.32% 134 96425 0.32% 135 98826 0.33% 136 98668 0.33% 137 101295 0.34% 138 101893 0.34% 139 104654 0.35% 140 106712 0.36% 141 111627 0.37% 142 111859 0.38% 143 114364 0.38% 144 117285 0.39% 145 115823 0.39% 146 119775 0.40% 147 254440 0.85% 148 107968 0.36% 149 109346 0.37% 150 25070081 84.16% 29789344 reads passed initial QC criterion=sequence-density sequence-density=0.63 sequence-density-rank=1 fanout-score=4.41 fanout-score-rank=21 prefix-density=0.85 prefix-fanout=3.3 sequence=TCTCCAGCTCCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=105.25 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=11.1 sequence=TCCTCCATGATGGCATTCTCCGGGTTGATCTCCATGGTCTTCTTGCTGGACATGTAACCAGCCATGCTAGAGTCCCTCAGGGCCTGGGCCTTCATGATCCTCTCCATGTTGGCAGTCCATCCATACTCACCAGTGACAAGGCAGCACGGCGAGTCAACAACACGGTCAGAGACAATGACCTTCTCCACCCTGTCGCCGAGCACCTCCTTGATAACCTTGCAGAGACCCTCAAACTTCTCCTTGAGCTCCTCCTTCTTTTTTTTCTCGTCCTCGGTGTCCTCGAGCTTCAGGCCTTCCTTGGTGGCAGAAACAAGTTTCTTGCCCTCAAACTCCTTGAGCTGGCCAATGGCATACTCATCAATGGCGTCAACCATATAGATAACCTCATAGCCCTTCTTCTTCAGCCTCTCCAGGAAGGGGGAGTTCTCCACAGCCTTCTTGC criterion=sequence-density sequence-density=0.45 sequence-density-rank=1 fanout-score=5.01 fanout-score-rank=29 prefix-density=0.63 prefix-fanout=3.6 sequence=GCCATGCTGCTA criterion=fanout-score sequence-density=0.04 sequence-density-rank=28 fanout-score=480.36 fanout-score-rank=1 prefix-density=1.01 prefix-fanout=17.8 sequence=CGCCGCCGCAGTCTCCGAGGAAACCCCCGCGACTCCGATGGCCGCCAGCAAATGGGTCCGGCCCGAGGTGTACCCGCTGTTCGCTGCGACGGGCGTGGCCGTCGGCATCTGCGCGTTCCAGCTGCTCCGCAACATCACTGGCAACCCGGAAGTCAGGGTTAGCAAGGTAGGGAGGGCAGCAGGAGTGCTTGATAATCATGAGGAGGGGAGGCGCTACGCTGAGCATGGTCTCAGAAGCTTTGTGCGTGACAAGACCCCCGAGATCATGCCAGGTATCAACAAGTTCTTCACCGACCCAAAGTGAAGGGTTTGGATTCGGCTATGACTCGCCAGTGCAAATGTTGTTCTGCTACTATTAGTTATGATTGTGGG ERR5262808 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:18:45 Started mapping on | Dec 06 12:18:46 Finished on | Dec 06 12:21:19 Mapping speed, Million of reads per hour | 700.93 Number of input reads | 29789344 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 28611126 Uniquely mapped reads % | 96.04% Average mapped length | 291.05 Number of splices: Total | 26780632 Number of splices: Annotated (sjdb) | 24717576 Number of splices: GT/AG | 26377977 Number of splices: GC/AG | 333233 Number of splices: AT/AC | 16627 Number of splices: Non-canonical | 52795 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.21 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 304480 % of reads mapped to multiple loci | 1.02% Number of reads mapped to too many loci | 938 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.91% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 873738 873738 873738 N_multimapping 304480 304480 304480 N_noFeature 1309507 27781833 1600254 N_ambiguous 636466 3904 98179 UnstrandedReadsAssigned:26665153 PositiveStrandReadsAssigned:825389 NegativeStrandReadsAssigned:26912693 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262808 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262808-trimmed-pair1.fastq ERR5262808-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,789,344 reads, 27,265,533 reads pseudoaligned [quant] estimated average fragment length: 273.058 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,185 rounds 52973 ERR5262808.ke.tsv 35125 ERR5262808.se.tsv 88098 total ==> ERR5262808.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 665.548 0 0 PNS24247 1044 771.942 156.399 10.3614 PNS24249 1928 1655.94 418.327 12.9193 PNS24246 1044 771.942 156.399 10.3614 PNS24248 1044 771.942 156.399 10.3614 PNS24244 1471 1198.94 173.475 7.39954 PNS24243 293 98.8109 3 1.55268 KQK14069 1603 1330.94 67438 2591.27 KQK14071 474 235.808 324.539 70.384 ==> ERR5262808.se.tsv <== BRADI_1g14170v3 68027 BRADI_1g53295v3 253 BRADI_1g59795v3 982 BRADI_1g07683v3 0 BRADI_1g00485v3 19 BRADI_1g20270v3 601 BRADI_1g74790v3 2536 BRADI_1g09890v3 0 BRADI_1g77505v3 324 BRADI_1g48960v3 0 ERR5262808 completed mapping pipeline successfully