Starting /dee2/code/volunteer_pipeline.sh ERR5262809 current disk space = 1551287083008 free memory = 1604061204 ERR5262809 SRAfilesize 433d31ba8f0d495719b98f617e6cee95 ERR5262809.sra ERR5262809.sra file validated ERR5262809 is paired end ERR5262809 is conventional basespace ERR5262809 read1 length is 76-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262809_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 76-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6565 37.0 37.0 37.0 37.0 37.0 2 36.5185 37.0 37.0 37.0 37.0 37.0 3 36.678 37.0 37.0 37.0 37.0 37.0 4 36.678 37.0 37.0 37.0 37.0 37.0 5 36.6925 37.0 37.0 37.0 37.0 37.0 6 36.727 37.0 37.0 37.0 37.0 37.0 7 36.752 37.0 37.0 37.0 37.0 37.0 8 36.698 37.0 37.0 37.0 37.0 37.0 9 36.668 37.0 37.0 37.0 37.0 37.0 10-14 36.6984 37.0 37.0 37.0 37.0 37.0 15-19 36.6477 37.0 37.0 37.0 37.0 37.0 20-24 36.611599999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.5831 37.0 37.0 37.0 37.0 37.0 30-34 36.5763 37.0 37.0 37.0 37.0 37.0 35-39 36.5271 37.0 37.0 37.0 37.0 37.0 40-44 36.4824 37.0 37.0 37.0 37.0 37.0 45-49 36.50599999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.46040000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.3848 37.0 37.0 37.0 37.0 37.0 60-64 36.4051 37.0 37.0 37.0 37.0 37.0 65-69 36.2328 37.0 37.0 37.0 37.0 37.0 70-74 36.208800000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.29569424712356 37.0 37.0 37.0 37.0 37.0 80-84 36.11495747873936 37.0 37.0 37.0 37.0 37.0 85-89 36.29927370490349 37.0 37.0 37.0 37.0 37.0 90-94 36.13111575423309 37.0 37.0 37.0 37.0 37.0 95-99 36.13130981724781 37.0 37.0 37.0 37.0 37.0 100-104 36.2421878091268 37.0 37.0 37.0 37.0 37.0 105-109 36.1923396652537 37.0 37.0 37.0 37.0 37.0 110-114 36.091044578430704 37.0 37.0 37.0 37.0 37.0 115-119 36.12115354131969 37.0 37.0 37.0 37.0 37.0 120-124 36.12574762213318 37.0 37.0 37.0 37.0 37.0 125-129 35.99830890850704 37.0 37.0 37.0 37.0 37.0 130-134 36.054582698321084 37.0 37.0 37.0 37.0 37.0 135-139 35.94445809614771 37.0 37.0 37.0 37.0 37.0 140-144 35.89259771647985 37.0 37.0 37.0 37.0 37.0 145-149 35.934541587251985 37.0 37.0 37.0 37.0 37.0 150 35.76945328319352 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 0.0 23 0.0 24 0.0 25 2.0 26 4.0 27 3.0 28 3.0 29 5.0 30 20.0 31 27.0 32 47.0 33 79.0 34 156.0 35 374.0 36 2799.0 37 479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.025000000000006 12.825000000000001 6.550000000000001 37.6 2 23.1615807903952 11.455727863931967 38.744372186093045 26.638319159579787 3 22.725 16.175 27.05 34.050000000000004 4 21.55 21.725 24.099999999999998 32.625 5 21.825 34.775 25.2 18.2 6 23.075000000000003 41.15 20.275000000000002 15.5 7 16.1 34.175 36.4 13.325000000000001 8 18.85 29.849999999999998 26.3 25.0 9 16.625 29.7 28.975 24.7 10-14 18.154999999999998 31.135 26.595000000000002 24.115000000000002 15-19 20.115 28.9 26.825 24.16 20-24 21.795 29.45 24.060000000000002 24.695 25-29 20.26 29.32 26.474999999999998 23.945 30-34 19.975 27.35 25.679999999999996 26.995 35-39 19.605 31.775 24.995 23.625 40-44 20.82 30.115 27.265 21.8 45-49 17.755000000000003 31.03 27.575 23.64 50-54 22.365 27.61 27.265 22.759999999999998 55-59 20.375 33.005 25.635 20.985 60-64 19.794999999999998 33.295 24.775 22.134999999999998 65-69 18.32 31.39 29.270000000000003 21.02 70-74 21.55 31.085 25.965 21.4 75-79 22.361708512553765 31.224367310193056 23.477043112933877 22.936881064319294 80-84 21.650825412706354 29.789894947473737 28.169084542271133 20.390195097548773 85-89 20.017010206123675 30.123073844306585 27.501500900540325 22.358415049029418 90-94 19.432489240316283 32.984686217595836 27.559803823441097 20.023020718646784 95-99 21.124574404165834 30.36751452032846 27.173042259162827 21.33486881634288 100-104 22.44887730553328 30.082197273456295 26.94967923015237 20.51924619085806 105-109 23.951795129299523 29.47024855636455 24.940999246798896 21.636957067537033 110-114 22.69765092194999 30.416771912099016 27.26446072240465 19.62111644354635 115-119 21.366998214741137 29.49757714868656 27.569497577148688 21.56592705942362 120-124 21.95109395109395 30.090090090090087 24.962676962676962 22.996138996138995 125-129 21.590968483771494 30.146866670151045 25.871530862907022 22.39063398317044 130-134 23.265089072055304 28.396703004520074 25.07311885136932 23.265089072055304 135-139 20.790471034109366 27.698971304818627 28.538170005414187 22.972387655657823 140-144 19.410143329658215 28.947078280044103 26.400220507166484 25.2425578831312 145-149 23.067735881248932 27.32184496388557 26.844110788830122 22.766308366035375 150 23.77784205958924 30.633497251952562 22.678623083598495 22.910037604859703 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 0.5 21 0.5 22 0.5 23 0.0 24 0.0 25 1.0 26 3.0 27 4.0 28 6.5 29 5.5 30 6.0 31 37.5 32 38.5 33 13.5 34 47.0 35 80.5 36 95.0 37 149.5 38 224.0 39 235.0 40 213.0 41 186.0 42 172.5 43 228.0 44 401.5 45 409.5 46 267.0 47 212.5 48 180.5 49 218.0 50 222.0 51 108.5 52 18.5 53 12.0 54 11.5 55 15.5 56 32.0 57 24.5 58 11.0 59 16.0 60 9.0 61 5.0 62 17.0 63 22.5 64 21.5 65 13.5 66 1.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 76-77 2.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.0 88-89 0.0 90-91 1.0 92-93 0.0 94-95 0.0 96-97 3.0 98-99 1.0 100-101 2.0 102-103 2.0 104-105 0.0 106-107 8.0 108-109 8.0 110-111 12.0 112-113 12.0 114-115 19.0 116-117 15.0 118-119 15.0 120-121 10.0 122-123 24.0 124-125 20.0 126-127 32.0 128-129 26.0 130-131 26.0 132-133 28.0 134-135 25.0 136-137 27.0 138-139 27.0 140-141 22.0 142-143 39.0 144-145 58.0 146-147 42.0 148-149 36.0 150-151 3457.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.674999999999997 #Duplication Level Percentage of deduplicated Percentage of total 1 43.135345666991235 11.075 2 18.597857838364167 9.55 3 11.002921129503408 8.475000000000001 4 6.134371957156767 6.3 5 4.284323271665044 5.5 6 3.79746835443038 5.8500000000000005 7 2.8237585199610513 5.075 8 0.9737098344693282 2.0 9 1.1684518013631937 2.7 >10 7.6923076923076925 36.925000000000004 >50 0.3894839337877313 6.550000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 74 1.8499999999999999 No Hit GTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 63 1.575 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 63 1.575 No Hit GCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACAC 62 1.55 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 50 1.25 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 46 1.15 No Hit CTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGA 43 1.075 No Hit GCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGC 39 0.975 No Hit CTCCAGTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTG 38 0.95 No Hit CCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAAC 37 0.9249999999999999 No Hit CTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATG 36 0.8999999999999999 No Hit TAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAG 34 0.8500000000000001 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 32 0.8 No Hit CTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCT 31 0.775 No Hit CTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGC 30 0.75 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 30 0.75 No Hit AGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCA 29 0.7250000000000001 No Hit CTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCAT 29 0.7250000000000001 No Hit GTGGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAAT 28 0.7000000000000001 No Hit GTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCA 28 0.7000000000000001 No Hit GTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAACATGCACG 27 0.675 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 24 0.6 No Hit GACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCC 23 0.575 No Hit GTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAG 22 0.5499999999999999 No Hit GTAGGAAGTTCTCCAACTAACCCGATTCCACTCGCTAAAAAGATTTGCAG 21 0.525 No Hit CTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACT 21 0.525 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 20 0.5 No Hit GTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCC 20 0.5 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 19 0.475 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 18 0.44999999999999996 No Hit CAGTGACTGACTCCTTACATAAATTAACTTAATTGAAAAATGACAAAGTA 18 0.44999999999999996 No Hit GGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAA 18 0.44999999999999996 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 18 0.44999999999999996 No Hit GCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATAT 18 0.44999999999999996 No Hit CGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCT 17 0.42500000000000004 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACA 17 0.42500000000000004 No Hit GTCCTTGGCAGCACAAGCAAAAGCAGTAAAAAAAGACAGGGCGTCGAAAT 17 0.42500000000000004 No Hit ATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAG 17 0.42500000000000004 No Hit CTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACC 17 0.42500000000000004 No Hit GGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAAT 17 0.42500000000000004 No Hit CTTTGGATGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTAT 17 0.42500000000000004 No Hit CATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCA 17 0.42500000000000004 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 16 0.4 No Hit GCTAGGGTAAATGCATTGCACCATTGTGAAACGAGAGGCCAATGGCCGAC 16 0.4 No Hit GGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAATTCGGAATGTTAT 16 0.4 No Hit GTTCTTTGGATGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACAT 15 0.375 No Hit GGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACG 15 0.375 No Hit AGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTA 15 0.375 No Hit CTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACA 14 0.35000000000000003 No Hit CATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAA 14 0.35000000000000003 No Hit CTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 14 0.35000000000000003 No Hit GGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCT 13 0.325 No Hit GTACAAAGAAGTTTTCATATTCCAGTCATAGGAAAAAATAGTGTGCAGGG 13 0.325 No Hit CAGGCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAG 13 0.325 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 13 0.325 No Hit CCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGG 13 0.325 No Hit CCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTCAC 12 0.3 No Hit ACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCA 12 0.3 No Hit GGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTCAG 12 0.3 No Hit AGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTTCAACTCCAGT 12 0.3 No Hit GTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAA 12 0.3 No Hit GCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAA 12 0.3 No Hit CCGGAATGCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGC 11 0.27499999999999997 No Hit ACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAG 11 0.27499999999999997 No Hit GGCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGC 11 0.27499999999999997 No Hit CAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTC 11 0.27499999999999997 No Hit CTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACC 11 0.27499999999999997 No Hit GGCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTC 11 0.27499999999999997 No Hit GCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATA 11 0.27499999999999997 No Hit CTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAAGCA 11 0.27499999999999997 No Hit CAGGCGAACAGCGACCAGGTAGCAAAGCCAACCAACAGGGACTGAGGGGC 11 0.27499999999999997 No Hit CTCTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGA 11 0.27499999999999997 No Hit ATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAGGC 11 0.27499999999999997 No Hit AGGAAGTTCTCCAACTAACCCGATTCCACTCGCTAAAAAGATTTGCAGCT 11 0.27499999999999997 No Hit GCACCATTGTGAAACGAGAGGCCAATGGCCGACGATATAGAGGTCCTTGG 10 0.25 No Hit CCTGCTGCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATG 10 0.25 No Hit GTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCTAAGCATCAT 10 0.25 No Hit TGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCTG 10 0.25 No Hit CTGGCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTC 10 0.25 No Hit GCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTT 10 0.25 No Hit GCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGC 10 0.25 No Hit AGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 10 0.25 No Hit CTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGA 10 0.25 No Hit CTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTTAATTGAAA 9 0.22499999999999998 No Hit GGTGACTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTTAAT 9 0.22499999999999998 No Hit ATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCT 9 0.22499999999999998 No Hit CCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTT 9 0.22499999999999998 No Hit GTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATA 9 0.22499999999999998 No Hit AGGCGAACAGCGACCAGGTAGCAAAGCCAACCAACAGGGACTGAGGGGCA 9 0.22499999999999998 No Hit CAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATGATGCACCT 9 0.22499999999999998 No Hit GTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGC 9 0.22499999999999998 No Hit CTGCGCGTTGCCGCCGCCCATGGCTTCTTTCTCCTCGGCGAGAACGAGAG 9 0.22499999999999998 No Hit GCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCT 9 0.22499999999999998 No Hit GTCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCG 9 0.22499999999999998 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 9 0.22499999999999998 No Hit AGCCATATGTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACA 8 0.2 No Hit AGCTAGGGTAAATGCATTGCACCATTGTGAAACGAGAGGCCAATGGCCGA 8 0.2 No Hit CCTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCT 8 0.2 No Hit GTCAACAAGATTGATTGTGCCATCACTGATGGCTGTGTAAATCTTAACAC 8 0.2 No Hit GTCACAACAGAAAATACACAGGACTGCAATTTACGGCCAGCAAAACCCAT 8 0.2 No Hit GTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTG 8 0.2 No Hit CTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGAT 8 0.2 No Hit GAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTAC 8 0.2 No Hit CTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCT 8 0.2 No Hit GGTAAATGCATTGCACCATTGTGAAACGAGAGGCCAATGGCCGACGATAT 8 0.2 No Hit CACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCA 7 0.17500000000000002 No Hit GGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTC 7 0.17500000000000002 No Hit CTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACA 7 0.17500000000000002 No Hit GTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAGATATTCTCTCTGCA 7 0.17500000000000002 No Hit GTGAAACGAGAGGCCAATGGCCGACGATATAGAGGTCCTTGGCAGCACAA 7 0.17500000000000002 No Hit GCCGGAGGTAATAAAGCTTCCATTTTCTAAAAAATAATAATCCAGATTCA 7 0.17500000000000002 No Hit ACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCC 7 0.17500000000000002 No Hit GGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCA 7 0.17500000000000002 No Hit CAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAATTC 7 0.17500000000000002 No Hit AGGGGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTT 7 0.17500000000000002 No Hit GTGCCATCCGACCACCCAACCAAATTACGCTCAAAAGCCAAGGGGCAACC 7 0.17500000000000002 No Hit ACTCTCCAAAGCAACCATTGAAAGCGCGTGCTGGATTATAATATTATAAG 7 0.17500000000000002 No Hit CGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTT 7 0.17500000000000002 No Hit CGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAG 7 0.17500000000000002 No Hit GGGCAGTTTACAATCTTCAGGTTCTGTAGTTCAGGAAACAAAGTTCTCAG 7 0.17500000000000002 No Hit CATGAAACAAGTAAAGCAAGAACTACATGCACTGCATGTAAACTAGGTAT 7 0.17500000000000002 No Hit GATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCGAGG 7 0.17500000000000002 No Hit GTGTAAATCTTAACACTCTCCAAAGCAACCATTGAAAGCGCGTGCTGGAT 7 0.17500000000000002 No Hit CTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCTAA 7 0.17500000000000002 No Hit ACCGCATACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTT 7 0.17500000000000002 No Hit GCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAAT 7 0.17500000000000002 No Hit CTTCAGGCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGTCA 7 0.17500000000000002 No Hit AGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCAT 7 0.17500000000000002 No Hit GTACAAGCGACAGCTAGGGTAAATGCATTGCACCATTGTGAAACGAGAGG 7 0.17500000000000002 No Hit CTCCGCGGGGGGAAAAGGCCTGCCGTTCGTTCGTTCGTTCGTCTCTCTAG 7 0.17500000000000002 No Hit GTTCGGACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 7 0.17500000000000002 No Hit CTAGATTGTAAAGCTAAATAAGAGTTCAACTCCAGTACAAAGAAGTTTTC 7 0.17500000000000002 No Hit CTCGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAA 7 0.17500000000000002 No Hit CCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAA 7 0.17500000000000002 No Hit CCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTT 6 0.15 No Hit CTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 6 0.15 No Hit GTTCTGTAGTTCAGGAAACAAAGTTCTCAGGTTCTTCTTCTCTGCTTGAC 6 0.15 No Hit GGTCCTTGGCAGCACAAGCAAAAGCAGTAAAAAAAGACAGGGCGTCGAAA 6 0.15 No Hit TTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTA 6 0.15 No Hit CCCAGCTCTGTCTTTTTTTAATCATCATCAAGGCTATTGATGATTTTATC 6 0.15 No Hit GCTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCA 6 0.15 No Hit ATCCAGATGCGTGTACAAACAATCTCTAACCCAGTAAAAATCGACAGCTA 6 0.15 No Hit GCTTTTTCTTTTTACTGCTTGGGCTGGCATTTTGCATGTTCCCATCCAAG 6 0.15 No Hit CTAGGGTAAATGCATTGCACCATTGTGAAACGAGAGGCCAATGGCCGACG 6 0.15 No Hit CCCAGTGACTGACTCCTTACATAAATTAACTTAATTGAAAAATGACAAAG 6 0.15 No Hit GGAAAGCTTATCTGGAGCTCGGCAAGGTAGTGCAAAGAATGAGCAAATCT 6 0.15 No Hit TTCCGGAATGCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGC 6 0.15 No Hit CTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTT 6 0.15 No Hit GTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTCAGA 6 0.15 No Hit GCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGCGGAT 6 0.15 No Hit ATTCGGAATGTTATTGCCATCAGTAGATTCGCCAAAAAAGTGCTCCCGGT 6 0.15 No Hit CTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAA 6 0.15 No Hit AACGACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGC 6 0.15 No Hit CGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATC 6 0.15 No Hit GCTGTGTAAATCTTAACACTCTCCAAAGCAACCATTGAAAGCGCGTGCTG 6 0.15 No Hit GCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAG 6 0.15 No Hit GTCATGTGTTTCAAGTTGTCATGAATAATGTAAGACTTTAGGCTGTATGC 6 0.15 No Hit CATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGC 6 0.15 No Hit ATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCT 6 0.15 No Hit CAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGG 6 0.15 No Hit GCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGT 6 0.15 No Hit ATCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 6 0.15 No Hit GCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAAC 6 0.15 No Hit GTTCGTCTCTCTAGGCTCTAGCACTAGCTGGGTGCGTGTGGGGGTCCTCG 6 0.15 No Hit CCCTGCTACAGTACATAAATTCGGAATGTTATTGCCATCAGTAGATTCGC 6 0.15 No Hit GGTGCATCCTCTGGGGCAGTTGATAGAACAATCGAGACATCTGGCTTTTT 6 0.15 No Hit CACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTTTT 6 0.15 No Hit GCCACTTTTTTATGCGTGTATGGTGTGAAGGATCCTCTTTCGGCCTCTTT 6 0.15 No Hit ACCACGCCGAGGCATACCGCATACACAATCTCGATCCATCAGCAGCTTTT 6 0.15 No Hit CACAAAATAATCATACTATTTCAGAGCTTCAGGCCTTCAGTGTCCAGATC 6 0.15 No Hit CTTGGCCTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGT 6 0.15 No Hit CTCAATTTTTTTTTAATCAGTCTTCTCGTAGAATGACCAATTGTCTCACA 6 0.15 No Hit AACCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATGATGCACCTT 6 0.15 No Hit CTTCCTCACCGGCGCCGGCCTCTCCATCTCTACCTCCGCGGGGGGAAAAG 5 0.125 No Hit GACTTCCTCACCGGCGCCGGCCTCTCCATCTCTACCTCCGCGGGGGGAAA 5 0.125 No Hit CTGAAAGAATGTCTTCAAGGACAATCGGCTTCGCTAGTTGCACTGTCGTG 5 0.125 No Hit GGGACATTATCCGGTGATCCAGGGATGAAAAACTTTGTGTGGTGGTTTTT 5 0.125 No Hit CCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCC 5 0.125 No Hit GTCAAGAATCTAGGCTAATAGAATTAGGAGCCCTGCTACAGTACATAAAT 5 0.125 No Hit AGCTTCAGGCCTTCAGTGTCCAGATCCAATTTATGGTATCCATTACAGGT 5 0.125 No Hit GAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTTCAACT 5 0.125 No Hit GCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATAC 5 0.125 No Hit TGTATGTTGACCTACACCTACACGCATATATATCTCTCGTGTGCACGGCT 5 0.125 No Hit CCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTA 5 0.125 No Hit GTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGC 5 0.125 No Hit CCAGTGACTGACTCCTTACATAAATTAACTTAATTGAAAAATGACAAAGT 5 0.125 No Hit CAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGCTAAATAAGAGTTC 5 0.125 No Hit CCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGA 5 0.125 No Hit TGGCGCCGCCGTGAGAATCGCCCGCGCCTCCCGAAGGTACCTGTCGATCA 5 0.125 No Hit CTTTGTTTAGACAGGTTTCTGGGATGGCGGATAGCATCAGATATTCTCTC 5 0.125 No Hit GTCCTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGC 5 0.125 No Hit GCTAAATAAGAGTTCAACTCCAGTACAAAGAAGTTTTCATATTCCAGTCA 5 0.125 No Hit GGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAG 5 0.125 No Hit ACACAATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCA 5 0.125 No Hit GGCCAATGGCCGACGATATAGAGGTCCTTGGCAGCACAAGCAAAAGCAGT 5 0.125 No Hit ACCAGATCCAATTTATGGTATCCATTACAGGTCACAGGCTAAGCATCATC 5 0.125 No Hit GTACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCG 5 0.125 No Hit CCCTGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 5 0.125 No Hit ACGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCC 5 0.125 No Hit GGCCCAGTGACTGACTCCTTACATAAATTAACTTAATTGAAAAATGACAA 5 0.125 No Hit GTGCTGGATTATAATATTATAAGATGATGCACCTTGTGGCTGGCAAGCAA 5 0.125 No Hit ATCTCGATCCATCAGCAGCTTTTTTCGCTTTTCGTTTTTTTTTCATCTTT 5 0.125 No Hit CCTGATGAAAACAAATAGCCATATGTACACACCATGCAAGTACCACGCCG 5 0.125 No Hit CCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGATCCATC 5 0.125 No Hit CGGCAAGGTAGTGCAAAGAATGAGCAAATCTGAAATCTAGATTGTAAAGC 5 0.125 No Hit GCACTTCTGACTCTTTATCCTGTATTTTTCTGAGGATCTTATCTTCAACA 5 0.125 No Hit CATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAACATGCACGTCA 5 0.125 No Hit CCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATACG 5 0.125 No Hit TGCTGCCACTTTGCGTATTGTACTTTGTTTAGACAGGTTTCTGGGATGGC 5 0.125 No Hit CACACAATGAAATACTCTGTATCCTTTTATATTGTCCAATAATGCAACCA 5 0.125 No Hit GGACAATCGGCTTCGCTAGTTGCACTGTCGTGCCATCCGACCACCCAACC 5 0.125 No Hit GTTAAATTTGAAATTGTGGTAGGAAGTTCTCCAACTAACCCGATTCCACT 5 0.125 No Hit ACACACCATGCAAGTACCACGCCGAGGCATACCGCATACACAATCTCGAT 5 0.125 No Hit GGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAA 5 0.125 No Hit CTGGAAGATCTGAAAGAATGTCTTCAAGGACAATCGGCTTCGCTAGTTGC 5 0.125 No Hit CCATTGAAAGCGCGTGCTGGATTATAATATTATAAGATGATGCACCTTGT 5 0.125 No Hit GTGACTTTAAACGACCCAGTGACTGACTCCTTACATAAATTAACTTAATT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAATGC 10 0.00744447 140.8875 8 TCTGCCT 20 0.0039598723 31.48324 135-139 CTCTGCC 25 0.003974278 31.417423 135-139 ATCTTCT 25 3.4335328E-4 30.879454 130-134 GATCTTC 25 3.4335328E-4 30.879454 130-134 TCTCTGC 35 0.0050766063 29.817461 135-139 TGGCGAT 30 0.0010636793 25.45393 125-129 CTCCTTG 30 0.0011693387 25.046667 120-124 TCTCCTT 30 0.0011693387 25.046667 120-124 CGCATTC 35 0.002957794 21.354681 115-119 GCATTCT 40 0.0064078835 18.685345 115-119 TCTACGC 40 0.0067099114 18.537828 110-114 TTCTACG 40 0.0067099114 18.537828 110-114 GTGAGGA 40 0.008278003 17.879124 100-104 TGAGGAG 40 0.008278003 17.879124 100-104 ATCCTGG 40 0.009036094 17.610937 45-49 GCCTCCT 40 0.009036094 17.610937 25-29 TGACCAC 40 0.009036094 17.610937 80-84 CCTGCCT 40 0.009036094 17.610937 30-34 GGCCGGG 40 0.009036094 17.610937 20-24 >>END_MODULE ERR5262809 read2 length is 76-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262809_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 76-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.122 37.0 37.0 37.0 37.0 37.0 2 36.1325 37.0 37.0 37.0 37.0 37.0 3 36.048 37.0 37.0 37.0 37.0 37.0 4 36.2395 37.0 37.0 37.0 37.0 37.0 5 36.356 37.0 37.0 37.0 37.0 37.0 6 36.2895 37.0 37.0 37.0 37.0 37.0 7 36.2145 37.0 37.0 37.0 37.0 37.0 8 36.2525 37.0 37.0 37.0 37.0 37.0 9 36.275 37.0 37.0 37.0 37.0 37.0 10-14 36.289 37.0 37.0 37.0 37.0 37.0 15-19 36.313900000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.2115 37.0 37.0 37.0 37.0 37.0 25-29 36.247499999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.1706 37.0 37.0 37.0 37.0 37.0 35-39 36.1432 37.0 37.0 37.0 37.0 37.0 40-44 36.06570000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.072300000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.0255 37.0 37.0 37.0 37.0 37.0 55-59 36.046 37.0 37.0 37.0 37.0 37.0 60-64 36.0237 37.0 37.0 37.0 37.0 37.0 65-69 36.009100000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.9467 37.0 37.0 37.0 37.0 37.0 75-79 35.90066378189094 37.0 37.0 37.0 37.0 37.0 80-84 35.85762881440721 37.0 37.0 37.0 37.0 37.0 85-89 35.880429631878734 37.0 37.0 37.0 37.0 37.0 90-94 35.77589773912015 37.0 37.0 37.0 37.0 37.0 95-99 35.72962066165591 37.0 37.0 37.0 37.0 37.0 100-104 35.73103471876395 37.0 37.0 37.0 37.0 37.0 105-109 35.591277418317205 37.0 37.0 37.0 37.0 37.0 110-114 35.61992826250258 37.0 37.0 37.0 37.0 37.0 115-119 35.53061753910247 37.0 37.0 37.0 37.0 37.0 120-124 35.522122585006564 37.0 37.0 37.0 34.6 37.0 125-129 35.44235615775664 37.0 37.0 37.0 34.6 37.0 130-134 35.35434602830754 37.0 37.0 37.0 32.2 37.0 135-139 35.25181473038375 37.0 37.0 37.0 27.4 37.0 140-144 35.20048212543038 37.0 37.0 37.0 25.0 37.0 145-149 35.1917479716148 37.0 37.0 37.0 27.4 37.0 150 34.99370709382151 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 5.0 22 3.0 23 6.0 24 5.0 25 2.0 26 10.0 27 11.0 28 13.0 29 15.0 30 18.0 31 44.0 32 65.0 33 134.0 34 266.0 35 669.0 36 2526.0 37 207.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.475 22.875 9.225 27.425 2 31.525 24.65 29.95 13.875000000000002 3 20.4 27.800000000000004 31.05 20.75 4 25.35 31.7 21.475 21.475 5 25.5 34.725 21.55 18.224999999999998 6 22.2 38.675 21.825 17.299999999999997 7 20.674999999999997 22.8 36.25 20.275000000000002 8 22.6 24.375 28.199999999999996 24.825 9 21.975 24.15 30.2 23.674999999999997 10-14 23.82 27.85 25.014999999999997 23.315 15-19 24.685000000000002 26.19 26.540000000000003 22.585 20-24 24.41 27.145000000000003 27.384999999999998 21.060000000000002 25-29 24.58 26.035000000000004 27.965 21.42 30-34 24.43 27.295 26.029999999999998 22.245 35-39 23.455000000000002 27.93 26.655 21.959999999999997 40-44 23.990000000000002 26.584999999999997 27.650000000000002 21.775 45-49 24.525 26.97 27.165 21.34 50-54 23.865 27.839999999999996 27.155 21.14 55-59 23.86 27.74 26.685 21.715 60-64 23.43 27.534999999999997 27.139999999999997 21.895 65-69 24.605 26.985 27.415 20.995 70-74 23.72 27.334999999999997 26.3 22.645 75-79 23.59707912373712 26.552965889766927 27.328198459537862 22.521756526958086 80-84 24.302151075537772 27.293646823411706 26.7983991995998 21.605802901450723 85-89 24.14948969381629 26.620972583550127 27.596557934760856 21.632979787872724 90-94 23.461115003503153 27.43969572615354 27.744970473426083 21.354218796917227 95-99 24.829761666332868 26.832565591828562 27.443420789104746 20.89425195273383 100-104 24.969927826784282 26.443464314354454 27.27044907778669 21.316158781074577 105-109 24.293246296761232 26.58799899573186 27.90861159929701 21.21014310820989 110-114 24.632482950239957 27.900985097246778 26.49659004799192 20.969941904521345 115-119 23.2479853106192 28.118943180658984 26.99683770274406 21.63623380597776 120-124 23.875450334534225 26.93772516726711 27.956767884714363 21.2300566134843 125-129 23.565680844393352 28.15341205977636 27.75107116731111 20.529835928519176 130-134 24.11060888061686 26.668439244881682 28.838074980058494 20.382876894442965 135-139 24.208475401850947 26.681820641879096 27.99155707095308 21.11814688531688 140-144 24.403679832534568 26.425384234010906 27.609761471933012 21.561174461521514 145-149 22.99898682877406 28.779691545648994 27.158617584149496 21.062704041427445 150 24.399313501144164 25.886727688787186 27.860411899313505 21.85354691075515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 1.5 21 1.0 22 0.0 23 0.5 24 0.5 25 0.0 26 0.0 27 2.0 28 2.0 29 6.0 30 9.0 31 6.0 32 10.0 33 18.5 34 25.5 35 35.5 36 60.0 37 97.5 38 135.5 39 181.5 40 187.5 41 241.5 42 290.0 43 263.0 44 293.0 45 282.0 46 225.5 47 209.5 48 193.0 49 165.0 50 112.5 51 76.0 52 87.5 53 99.0 54 97.0 55 85.5 56 69.0 57 39.0 58 60.5 59 73.5 60 38.5 61 29.5 62 24.5 63 19.0 64 15.5 65 13.0 66 14.5 67 17.0 68 21.0 69 22.5 70 17.5 71 9.5 72 5.0 73 2.5 74 1.0 75 2.0 76 2.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 76-77 2.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.0 88-89 0.0 90-91 1.0 92-93 0.0 94-95 0.0 96-97 3.0 98-99 1.0 100-101 2.0 102-103 2.0 104-105 0.0 106-107 8.0 108-109 8.0 110-111 12.0 112-113 12.0 114-115 19.0 116-117 15.0 118-119 14.0 120-121 10.0 122-123 24.0 124-125 20.0 126-127 32.0 128-129 27.0 130-131 26.0 132-133 28.0 134-135 25.0 136-137 24.0 138-139 27.0 140-141 23.0 142-143 37.0 144-145 19.0 146-147 42.0 148-149 40.0 150-151 3496.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.625 #Duplication Level Percentage of deduplicated Percentage of total 1 53.67609254498714 26.1 2 22.31362467866324 21.7 3 11.15681233933162 16.275000000000002 4 5.552699228791774 10.8 5 3.033419023136247 7.375 6 1.5938303341902313 4.65 7 0.7712082262210797 2.625 8 0.5141388174807198 2.0 9 0.2056555269922879 0.8999999999999999 >10 1.1825192802056554 7.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCACTCCACTCTCTCTCTCTCTGTTCGCCTCTCGCCTTTTTAGAGAGA 23 0.575 No Hit GTCATTTTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGA 18 0.44999999999999996 No Hit ATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTG 17 0.42500000000000004 No Hit AAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCCTC 17 0.42500000000000004 No Hit GTTCGACTCCACTCCACTCTCTCTCTCTCTGTTCGCCTCTCGCCTTTTTA 15 0.375 No Hit GTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAGTCTTCAGTGACTTC 15 0.375 No Hit GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 14 0.35000000000000003 No Hit AAACAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTGTGTC 14 0.35000000000000003 No Hit TGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTT 13 0.325 No Hit TGTTAGTGCAACTTTTGTTCTCAGAGCAGTCGGCATCCATCTTGACCAGA 13 0.325 No Hit GTTCATACTCAAGCAGGAGGAAATAGTCATCAGCGCTTCTATATTCATGT 12 0.3 No Hit CAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCCAGA 12 0.3 No Hit GTTTCTGAGTTTTGTGGAAGTCTTTTTGCCTAGCTTGTTTCTGTACTTCT 12 0.3 No Hit GATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGGTCAT 12 0.3 No Hit CGGAGAGGCAGCCACCACCAGCGCAGCATCTCATCGGTCACATCGTCACA 12 0.3 No Hit ATGGATTCCAGGAGTCTTCAGTGACTTCTGCCATGCCAAAAAAAAGATGA 11 0.27499999999999997 No Hit CCAACACGTGCGAAATCACCAAAGTTTACAAGGAGAAAGAGCTACGGTGA 11 0.27499999999999997 No Hit GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 11 0.27499999999999997 No Hit GGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAAT 11 0.27499999999999997 No Hit GTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTT 10 0.25 No Hit GTTCGCGTTACATCCAGGTATTCAAGTTTCTTCTACCCAGATGTATTTGC 10 0.25 No Hit CAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTC 10 0.25 No Hit CATGCCCTTTGGCCTGTTGCCATCGGTGTGTATATCACTAGTTTTTGTTT 10 0.25 No Hit AGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATT 9 0.22499999999999998 No Hit GATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACT 9 0.22499999999999998 No Hit GTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGA 9 0.22499999999999998 No Hit CCCAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTG 9 0.22499999999999998 No Hit GGAAAAGCTACAGCTTCTCAACTCTTCTCAAGAAAGAGCGCGCAGAATAA 8 0.2 No Hit CTTACATTGCATAGGTCTCTCAATTGTTTCTTTGGCGTGAGTCCGTTCTC 8 0.2 No Hit CGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGTGGTC 8 0.2 No Hit GTCTGGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTG 8 0.2 No Hit CTACGGTGATACTCCGCCAAACGGAGAGGCAGCCACCACCAGCGCAGCAT 8 0.2 No Hit ATCTCATCGGTCACATCGTCACAGCATTGGGAACCCTAAAGATGCCAACA 8 0.2 No Hit CACAGGTTTCAACTCTAATTTGTGATTCTAAGCAACTTAAGAAGCTGCTT 8 0.2 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 8 0.2 No Hit GGATTGACTATTACTTGGAGTATGAACCTCATCCATCATCAACTAAAAAC 8 0.2 No Hit AGAAGACAGAGGGGGAGCTTTTTGAGACAGAGAAGGAGACTACCAAGAAT 8 0.2 No Hit AGGAGAAAGAGCTACGGTGATACTCCGCCAAACGGAGAGGCAGCCACCAC 7 0.17500000000000002 No Hit GTCATTTTACATGGTTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGA 7 0.17500000000000002 No Hit AGGGTGTATTGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTAC 7 0.17500000000000002 No Hit GAATGGATGAGCTCAGTGAGGATGATAAGTTAACAGTTGCTCGCGCTAGG 7 0.17500000000000002 No Hit GACAGATTTCTGAGCTTGGTATTTATCCTGCTGTCGATCCTTTGGACTCC 7 0.17500000000000002 No Hit GGAAAATATACAATGTGGTGGATGACGATCCTGCCCCAAGAGCTGATGTT 7 0.17500000000000002 No Hit TGAAGAGCTATAACCATCAGTGTTGATGCCTCTCTCACACACAAATGGAA 7 0.17500000000000002 No Hit GGAGAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGGTCCACCG 7 0.17500000000000002 No Hit GGAAAATTCAGCGGTTCCTTAGCCAGCCTTTTCATGTTGCTGAAGTGTTC 7 0.17500000000000002 No Hit GTCCGTTCTCTCCCCCATCCCCTGCACACTATTTTTTCCTATGACTGGAA 7 0.17500000000000002 No Hit GAAAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCC 7 0.17500000000000002 No Hit CATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTTCCATTGG 7 0.17500000000000002 No Hit GACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTT 7 0.17500000000000002 No Hit AATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGT 7 0.17500000000000002 No Hit GTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTAT 7 0.17500000000000002 No Hit GGTGGTCCACCGCTCCAAGGTGGAGCAGGGATGTCTCGAGTATTTGTGCC 6 0.15 No Hit CAAACTACTACCTATGCAATATCGATAACAGGAACATCTTTTGTGTCCTG 6 0.15 No Hit CTTCATGTTCGTCAACTGGACCTTCCTGTGATCCGACATACTGCATATTT 6 0.15 No Hit GCTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAA 6 0.15 No Hit CCTAAAGATGCCAACAAAGCGCAATGCTCGCCAAAGAATGGTGTAGCTGC 6 0.15 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 6 0.15 No Hit GTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATG 6 0.15 No Hit CACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGAAGTC 6 0.15 No Hit CAAGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAGAGA 6 0.15 No Hit GGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGGTCTCTCAA 6 0.15 No Hit GGCACGACCATTCAAAAGATTGAGGGTTCATACTCAAGCAGGAGGAAATA 6 0.15 No Hit AGAAGTTTGATGACAAGTACTTTGCTAGGGACAAGAAGGCAAGGGCAAAG 6 0.15 No Hit CAAGCAATCTTCCCCAAAGGGTTTAGGAGCAGAGTAAAATACTTCAGCAT 6 0.15 No Hit CTTCCTCGCCGAGAAGGGGAACGGAAGCGCCGACCAGAACCGAGGGCGAG 6 0.15 No Hit ATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACT 6 0.15 No Hit AGCAGGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGC 6 0.15 No Hit GTTCTCTCCCCCATCCCCTGCACACTATTTTTTCCTATGACTGGAATATG 6 0.15 No Hit GTCTTTTTGCCTAGCTTGTTTCTGTACTTCTGAACTGTGTACCCCGGTAG 6 0.15 No Hit CACCATACCTACCTATATAGTGCATCTCTCGCTATTCCATAGTAGTTGAT 6 0.15 No Hit GTGTTGATGCCTCTCTCACACACAAATGGAAAAAAAAATGGAAAAAAAAA 6 0.15 No Hit GCCCAACCAAGAATGTTCCGAACTGCAGTGCAATAGATGGATTCCAGGAG 6 0.15 No Hit ATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAA 6 0.15 No Hit TGATCGATAGGGAGGAGTGCTTCTCGCATAACGTGGAGTACGAGGGGGAC 6 0.15 No Hit GATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGGT 6 0.15 No Hit AGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGT 6 0.15 No Hit AGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTT 6 0.15 No Hit CTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAA 6 0.15 No Hit GTTTCTTCTACCCAGATGTATTTGCAACCCTGAGACTGAATGCAATGGAA 6 0.15 No Hit GTGGAGTCAGATCCTCCGAGGACTCGGGAACTTGATACTGTTGGTGTGCT 6 0.15 No Hit TATGCTTTATATTTAGAAGAGAGACTTGAATGTTTCCGAGTGCTGAAGTA 6 0.15 No Hit GTTTCTTTGGCGTGAGTCCGTTCTCTCCCCCATCCCCTGCACACTATTTT 6 0.15 No Hit CTGGAAAGTATGTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTA 5 0.125 No Hit CAGACCTTAAAAACTATCTTGGTGCCCGGTTCTCTCTCAGGGACGGTGAC 5 0.125 No Hit AGAAGGGGAACGGAAGCGCCGACCAGAACCGAGGGCGAGCGGCGGTAGTT 5 0.125 No Hit GTGCTGACAAGAGGAAGGAGTTCTACACGAAACTAGAAGAGAAACACAAA 5 0.125 No Hit GGATTATCAAAAAGCTGTTGTACGAATTGTAAATGGGAAAAAATCAGACG 5 0.125 No Hit ATTATAGCTGCTGAAAAACGGGTCTCAAATGAACGGTTGAAACAGGAACT 5 0.125 No Hit TGTCGATGTTGTATGTGGAGGTCCACCATGCCAAGGTATTAGTGGCTACA 5 0.125 No Hit GGATCATTGCTGCCCAGGGATGCTTTCGACAAAATTTAACTGTTGTAACC 5 0.125 No Hit GGTTGACATCTCTGGTGTTAAGGTGGAGAAGTTTGATGACAAGTACTTTG 5 0.125 No Hit CCAAAGTTTACAAGGAGAAAGAGCTACGGTGATACTCCGCCAAACGGAGA 5 0.125 No Hit ATTCTTTTTGGTCGTCAACTGCAGGTCATGACCATCAAATTGAGCTCAAG 5 0.125 No Hit GGGAACGGAAGCGCCGACCAGAACCGAGGGCGAGCGGCGGTAGTTCTCCG 5 0.125 No Hit GAATAAATGAAGTTCTAGAAGTGCATGTTGACCCCCATATGGATCCTGAT 5 0.125 No Hit AAGATATCCTGACCTTGCGATGGATCCGGCAAGATCAAACATTCATGATA 5 0.125 No Hit GGAATATATGGGCCTGGAAGAAGTGCTTTGGACACAATAGCTAAGGGTAA 5 0.125 No Hit GGGATGTCTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTG 5 0.125 No Hit GATGTGTCAATGTTTAAAAAGCATCTAGATACATGTAATATTTCGACAAG 5 0.125 No Hit TGCATCTTTTCTTATGCATGAGGGTGCTGAGCTATGGACTGTTCGCGTTA 5 0.125 No Hit CGAGAAGATTCAAAATTGCTTTATTGTCATTGACATCAATCCTGCAAATT 5 0.125 No Hit GGTGCTGAGCTATGGACTGTTCGCGTTACATCCAGGTATTCAAGTTTCTT 5 0.125 No Hit CACGCCGTGGCATTATAGCGAGGATGGGGTGGATCTTGTTGTTAAAGATC 5 0.125 No Hit GATCAAATAAATGAGGCATGCAAATTCTTGGATGAAAGTTGGTCCCCCAA 5 0.125 No Hit GTCAACCAGACTTATGTTATTGCCACATCCACAAAGGTTGACATCTCTGG 5 0.125 No Hit CAATTATTTTATGGAATTGGATCTTACTCCTTCCATACCATATTAAGTGA 5 0.125 No Hit AGAGCTTATTAATTATGGCAGATCTAGGTCACACATGCTAAACATGGCCT 5 0.125 No Hit GGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAG 5 0.125 No Hit GCAATATCGATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATT 5 0.125 No Hit GAGAAACACAAAGCTTTGGAAGCTGAGAAGGACCAGGCTGAAACAAGGAA 5 0.125 No Hit GACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCATTG 5 0.125 No Hit TGGGCGGTTGCACTGAAGACATTAATTGTCATACACCGTGCCCTTCGAGA 5 0.125 No Hit CCCATTTCCTCTGCCTACACATGAAGCAATTCGTAAAAATGGTTGCCCCT 5 0.125 No Hit GGTGCCTCCAACACGTGCGAAATCACCAAAGTTTACAAGGAGAAAGAGCT 5 0.125 No Hit GTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCC 5 0.125 No Hit ATGCACTGGTGCATTCACTAAATGAGACACAGGTTTCAACTCTAATTTGT 5 0.125 No Hit CAAGAAGGTGCCTCCAACACGTGCGAAATCACCAAAGTTTACAAGGAGAA 5 0.125 No Hit CGACGTGGCAGGCGCCCCGCACTGCCTCGCCTTCATGTTCGTCAACTGGA 5 0.125 No Hit AGCATAAAATGCCTCGACCTGAAGGTTGATGGGCCATGATTGGTGGACTA 5 0.125 No Hit ATCTAAACAAAAAAGAGATTATCACGATGGATACAATATCAAATCAGGAC 5 0.125 No Hit GCTGGAGACGACGCTGGTCAACCTGCAGGACATCTCGCTGGAGGCGATCC 5 0.125 No Hit AGTTGCTATGCGTTACCAAGCCAAACTAGGGATCATGGCTGCTGGATGTT 5 0.125 No Hit AGGAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACC 5 0.125 No Hit TGAGGAGGTGGTGGTCATCAAGGGTGCTGGGCACTTCATCCAGCAGGAGA 5 0.125 No Hit GGAAAACAAGGAGATCATTTTCTTTATGGATGTCATGCAATTCTTGAAGC 5 0.125 No Hit GTTTGATGACAAGTACTTTGCTAGGGACAAGAAGGCAAGGGCAAAGAAGA 5 0.125 No Hit CGTTGGAGTAAAGGCCATTCAACTTTGTCTGTTCATGATAAGCTTTTCTG 5 0.125 No Hit GAGAAATTTTCCATTCCATTTCTGCTGCAAGGCCACGGGCAGATGTAGCT 5 0.125 No Hit CAAATGGAAAAAAAAATGGAAAAAAAAATGAAAAGAGAGGGGCACGACCA 5 0.125 No Hit CGATAACAGGAACATCTTTTGTGTCCTGGGATCACCTTACATTGCATAGG 5 0.125 No Hit GATGGATTCCAGGAGTCTTCAGTGACTTCTGCCATGCCAAAAAAAAGATG 5 0.125 No Hit CATGCAAATTCTTGGATGAAAGTTGGTCCCCCAAATTCACACTAATTGTT 5 0.125 No Hit GAATTGGCTTGCGCCCTGTTGAGGTTTGCAGGTCTGGTGGTCCACCGCTC 5 0.125 No Hit CAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACT 5 0.125 No Hit AGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTA 5 0.125 No Hit CGCAAATGGATGGCACAATGGAGACCACCCTGCTTCACCCTGGATGATGG 5 0.125 No Hit GGACAAGAAGGCAAGGGCAAAGAAGACAGAGGGGGAGCTTTTTGAGACAG 5 0.125 No Hit GGCTATTGAGGCCGTCCCAGACCTTAAAAACTATCTTGGTGCCCGGTTCT 5 0.125 No Hit CCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGAA 5 0.125 No Hit TGTGATTCTAAGCAACTTAAGAAGCTGCCTTCAGTCAGTTCGAAGTTGCA 5 0.125 No Hit CTCGAGTATTTGTGCCCAACCAAGAATGTTCCGAACTGCAGTGCAATAGA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTTA 10 0.0074306633 140.975 9 TATGCGT 10 0.0074306633 140.975 7 GCTATGC 10 0.0074306633 140.975 5 TGCTATG 10 0.0074306633 140.975 4 CTATGCG 10 0.0074306633 140.975 6 >>END_MODULE Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018281 spots for ERR5262809.sra Written 2018281 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra Read 2018264 spots for ERR5262809.sra Written 2018264 spots for ERR5262809.sra SRR ids: ['ERR5262809.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_yy0tfu2i ERR5262809.sra spots: 40365297 blocks: [[1, 2018264], [2018265, 4036528], [4036529, 6054792], [6054793, 8073056], [8073057, 10091320], [10091321, 12109584], [12109585, 14127848], [14127849, 16146112], [16146113, 18164376], [18164377, 20182640], [20182641, 22200904], [22200905, 24219168], [24219169, 26237432], [26237433, 28255696], [28255697, 30273960], [30273961, 32292224], [32292225, 34310488], [34310489, 36328752], [36328753, 38347016], [38347017, 40365297]] ERR5262809 file size 13239324 ERR5262809 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262809 ERR5262809_1.fastq ERR5262809_2.fastq Input file: ERR5262809_1.fastq Paired file: ERR5262809_2.fastq trimmed: ERR5262809-trimmed-pair1.fastq, ERR5262809-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:24:56 2024 >> started Fri Dec 6 12:25:37 2024 >> done (40.561s) 40365297 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 40365296 (100.00%) read pairs available; of these: 10692 ( 0.03%) trimmed read pairs available after processing 40354604 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 2 0.00% 26 1 0.00% 27 5 0.00% 28 1 0.00% 29 2 0.00% 30 1 0.00% 31 2 0.00% 32 2 0.00% 33 4 0.00% 34 5 0.00% 35 4 0.00% 36 4 0.00% 37 5 0.00% 38 4 0.00% 39 7 0.00% 40 0 0.00% 41 1 0.00% 42 3 0.00% 43 1 0.00% 44 4 0.00% 45 2 0.00% 46 1 0.00% 47 5 0.00% 48 5 0.00% 49 352 0.00% 50 375 0.00% 51 373 0.00% 52 482 0.00% 53 543 0.00% 54 523 0.00% 55 531 0.00% 56 639 0.00% 57 645 0.00% 58 828 0.00% 59 917 0.00% 60 1085 0.00% 61 1300 0.00% 62 1507 0.00% 63 1558 0.00% 64 1738 0.00% 65 1848 0.00% 66 2067 0.01% 67 2254 0.01% 68 2426 0.01% 69 3053 0.01% 70 3475 0.01% 71 4027 0.01% 72 4544 0.01% 73 5400 0.01% 74 5856 0.01% 75 6560 0.02% 76 7160 0.02% 77 7832 0.02% 78 8879 0.02% 79 9857 0.02% 80 10895 0.03% 81 12485 0.03% 82 14276 0.04% 83 16009 0.04% 84 17839 0.04% 85 20009 0.05% 86 21505 0.05% 87 22994 0.06% 88 24867 0.06% 89 26711 0.07% 90 29018 0.07% 91 32233 0.08% 92 34569 0.09% 93 37879 0.09% 94 41581 0.10% 95 44035 0.11% 96 47342 0.12% 97 49689 0.12% 98 52044 0.13% 99 53763 0.13% 100 56473 0.14% 101 59817 0.15% 102 62488 0.15% 103 67227 0.17% 104 70336 0.17% 105 73605 0.18% 106 77355 0.19% 107 79237 0.20% 108 81322 0.20% 109 84365 0.21% 110 87029 0.22% 111 89617 0.22% 112 92628 0.23% 113 96630 0.24% 114 99775 0.25% 115 103568 0.26% 116 106242 0.26% 117 108682 0.27% 118 111411 0.28% 119 112702 0.28% 120 114318 0.28% 121 116155 0.29% 122 119165 0.30% 123 120722 0.30% 124 125786 0.31% 125 126292 0.31% 126 129907 0.32% 127 133185 0.33% 128 133832 0.33% 129 134809 0.33% 130 136558 0.34% 131 137212 0.34% 132 140189 0.35% 133 141148 0.35% 134 144101 0.36% 135 147633 0.37% 136 149550 0.37% 137 149928 0.37% 138 151572 0.38% 139 153833 0.38% 140 155209 0.38% 141 157330 0.39% 142 160665 0.40% 143 160758 0.40% 144 163091 0.40% 145 164161 0.41% 146 167342 0.41% 147 314483 0.78% 148 160981 0.40% 149 159769 0.40% 150 33476653 82.93% 40365296 reads passed initial QC criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=6.65 fanout-score-rank=31 prefix-density=0.32 prefix-fanout=3.5 sequence=GCCGCCCTTGGGGG criterion=fanout-score sequence-density=0.03 sequence-density-rank=22 fanout-score=148.31 fanout-score-rank=1 prefix-density=0.63 prefix-fanout=7.0 sequence=CCTTCTTCTTCGTCTCCGGCGACGTCGTCTTGTCGGCGGCCTTGAGCTTGCGTGTGAGTGTGCGCCAGTAGTTCTTGATCTCGTTGTCGGTGCGGCCGGGGAGCCTCCGTGCGATGCGTGACCATCGGCTGCCCCACTGGGAGTGGAGCTGCAGGATGAGGCGCTCCTCGTCGGGCGTGATCCGGCCGCGCTTCAGCCCTGGGTGCAGGTAGTTCACCCACCGGAGACG criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=2.61 fanout-score-rank=32 prefix-density=0.21 prefix-fanout=2.4 sequence=ATCAAGCCCGAC criterion=fanout-score sequence-density=0.10 sequence-density-rank=13 fanout-score=126.38 fanout-score-rank=1 prefix-density=0.89 prefix-fanout=15.0 sequence=CGCCGCCGCCGTC ERR5262809 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:27:01 Started mapping on | Dec 06 12:27:02 Finished on | Dec 06 12:29:25 Mapping speed, Million of reads per hour | 1016.19 Number of input reads | 40365296 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 34657400 Uniquely mapped reads % | 85.86% Average mapped length | 290.07 Number of splices: Total | 34272643 Number of splices: Annotated (sjdb) | 31803236 Number of splices: GT/AG | 33806553 Number of splices: GC/AG | 389941 Number of splices: AT/AC | 22820 Number of splices: Non-canonical | 53329 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.27 Insertion rate per base | 0.01% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 377884 % of reads mapped to multiple loci | 0.94% Number of reads mapped to too many loci | 372 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 13.19% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5330012 5330012 5330012 N_multimapping 377884 377884 377884 N_noFeature 1370229 33761197 1645725 N_ambiguous 817269 6710 196771 UnstrandedReadsAssigned:32469902 PositiveStrandReadsAssigned:889493 NegativeStrandReadsAssigned:32814904 Dataset is classified negative stranded MeadianReadLen=146 20thPercentileLength=146 echo kmer=141 ERR5262809 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262809-trimmed-pair1.fastq ERR5262809-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 40,365,296 reads, 37,604,129 reads pseudoaligned [quant] estimated average fragment length: 271.232 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,493 rounds 52973 ERR5262809.ke.tsv 35125 ERR5262809.se.tsv 88098 total ==> ERR5262809.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 666.766 0 0 PNS24247 1044 773.768 222.186 11.0151 PNS24249 1928 1657.77 516.587 11.9537 PNS24246 1044 773.768 222.186 11.0151 PNS24248 1044 773.768 222.186 11.0151 PNS24244 1471 1200.77 264.855 8.4612 PNS24243 293 104.095 0 0 KQK14069 1603 1332.77 41566.5 1196.39 KQK14071 474 239.09 177.948 28.5506 ==> ERR5262809.se.tsv <== BRADI_1g14170v3 36993 BRADI_1g53295v3 287 BRADI_1g59795v3 866 BRADI_1g07683v3 0 BRADI_1g00485v3 34 BRADI_1g20270v3 1322 BRADI_1g74790v3 2634 BRADI_1g09890v3 0 BRADI_1g77505v3 426 BRADI_1g48960v3 0 ERR5262809 completed mapping pipeline successfully