Starting /dee2/code/volunteer_pipeline.sh ERR5262810 current disk space = 1551232827392 free memory = 1599031232 ERR5262810 SRAfilesize 3c145e57ca869d934c5a1037b3bd2599 ERR5262810.sra ERR5262810.sra file validated ERR5262810 is paired end ERR5262810 is conventional basespace ERR5262810 read1 length is 106-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262810_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 106-150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7695 37.0 37.0 37.0 37.0 37.0 2 36.6695 37.0 37.0 37.0 37.0 37.0 3 36.775 37.0 37.0 37.0 37.0 37.0 4 36.718 37.0 37.0 37.0 37.0 37.0 5 36.7645 37.0 37.0 37.0 37.0 37.0 6 36.728 37.0 37.0 37.0 37.0 37.0 7 36.6905 37.0 37.0 37.0 37.0 37.0 8 36.787 37.0 37.0 37.0 37.0 37.0 9 36.7595 37.0 37.0 37.0 37.0 37.0 10-14 36.7196 37.0 37.0 37.0 37.0 37.0 15-19 36.686099999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.677499999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.6678 37.0 37.0 37.0 37.0 37.0 30-34 36.6389 37.0 37.0 37.0 37.0 37.0 35-39 36.6642 37.0 37.0 37.0 37.0 37.0 40-44 36.61990000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.6354 37.0 37.0 37.0 37.0 37.0 50-54 36.5881 37.0 37.0 37.0 37.0 37.0 55-59 36.604400000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.5525 37.0 37.0 37.0 37.0 37.0 65-69 36.5401 37.0 37.0 37.0 37.0 37.0 70-74 36.5055 37.0 37.0 37.0 37.0 37.0 75-79 36.5545 37.0 37.0 37.0 37.0 37.0 80-84 36.5247 37.0 37.0 37.0 37.0 37.0 85-89 36.5382 37.0 37.0 37.0 37.0 37.0 90-94 36.4621 37.0 37.0 37.0 37.0 37.0 95-99 36.4704 37.0 37.0 37.0 37.0 37.0 100-104 36.5044 37.0 37.0 37.0 37.0 37.0 105-109 36.56673252346764 37.0 37.0 37.0 37.0 37.0 110-114 36.43766930117816 37.0 37.0 37.0 37.0 37.0 115-119 36.49182011982042 37.0 37.0 37.0 37.0 37.0 120-124 36.49679397680944 37.0 37.0 37.0 37.0 37.0 125-129 36.464277126345614 37.0 37.0 37.0 37.0 37.0 130-134 36.438629322696805 37.0 37.0 37.0 37.0 37.0 135-139 36.34990983818214 37.0 37.0 37.0 37.0 37.0 140-144 36.39911616947965 37.0 37.0 37.0 37.0 37.0 145-149 36.32057899384866 37.0 37.0 37.0 37.0 37.0 150 36.20946416144746 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 1.0 28 3.0 29 9.0 30 9.0 31 25.0 32 30.0 33 37.0 34 67.0 35 162.0 36 2575.0 37 1081.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.575 44.375 2.1 32.95 2 28.342513770655987 22.458688032048073 23.184777165748624 26.01402103154732 3 10.4 28.9 14.249999999999998 46.45 4 24.0 29.525000000000002 25.75 20.724999999999998 5 24.45 27.650000000000002 23.7 24.2 6 21.85 35.35 25.35 17.45 7 18.275 21.775 40.575 19.375 8 19.950000000000003 23.5 29.95 26.6 9 17.75 20.375 37.7 24.175 10-14 25.124999999999996 23.54 26.605 24.73 15-19 22.720000000000002 22.365 28.610000000000003 26.305 20-24 24.52 23.380000000000003 26.834999999999997 25.264999999999997 25-29 22.935 25.235000000000003 26.700000000000003 25.130000000000003 30-34 21.224999999999998 24.73 25.755 28.29 35-39 22.825 26.195 25.590000000000003 25.39 40-44 24.07 24.575 25.61 25.745 45-49 23.135 24.8 25.45 26.615 50-54 23.26 25.72 26.790000000000003 24.23 55-59 26.39 24.365000000000002 25.145 24.099999999999998 60-64 25.215 25.355 26.33 23.1 65-69 27.095000000000002 25.509999999999998 24.89 22.505 70-74 25.21 24.725 26.045 24.02 75-79 27.065 25.069999999999997 25.785000000000004 22.08 80-84 25.735000000000003 26.119999999999997 25.11 23.035 85-89 26.505000000000003 25.595000000000002 24.8 23.1 90-94 25.55 25.900000000000002 25.290000000000003 23.26 95-99 28.17 25.740000000000002 24.240000000000002 21.85 100-104 24.035 27.939999999999998 26.284999999999997 21.740000000000002 105-109 26.264363323692645 24.36488704760416 22.51231142030798 26.85843820839522 110-114 27.09856314595412 30.3629947063272 25.23317368288379 17.30526846483489 115-119 29.515474378488076 29.3505834601725 22.247590055809233 18.88635210553019 120-124 26.78617157490397 35.80025608194622 20.128040973111396 17.285531370038413 125-129 22.37708091366628 32.97199638663053 21.189830945928506 23.461091753774678 130-134 19.26892950391645 30.822454308093995 24.556135770234988 25.35248041775457 135-139 20.809326008307654 26.316494707222297 30.21573093930055 22.658448345169504 140-144 19.641401792991033 27.166530834012498 26.514534093996193 26.677533279000272 145-149 19.814840403482105 31.325134724333285 22.536962829901892 26.323062042282714 150 18.16283924843424 32.84620737647877 29.15796798886569 19.832985386221296 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 1.0 29 2.0 30 2.5 31 8.5 32 14.5 33 14.5 34 24.5 35 35.0 36 38.0 37 51.0 38 64.0 39 73.0 40 104.5 41 130.0 42 150.0 43 169.5 44 157.0 45 140.0 46 141.5 47 144.0 48 167.0 49 260.5 50 349.0 51 344.0 52 337.0 53 256.0 54 142.0 55 115.0 56 80.0 57 52.0 58 50.5 59 43.5 60 33.0 61 32.0 62 27.5 63 23.5 64 18.5 65 15.0 66 17.5 67 31.5 68 35.0 69 26.5 70 24.0 71 13.5 72 11.0 73 13.5 74 7.0 75 4.5 76 2.5 77 0.5 78 0.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.15 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 106 2399.0 107 2.0 108 6.0 109 0.0 110 4.0 111 4.0 112 1.0 113 1.0 114 1.0 115 2.0 116 2.0 117 4.0 118 4.0 119 5.0 120 0.0 121 2.0 122 2.0 123 5.0 124 1.0 125 3.0 126 0.0 127 3.0 128 8.0 129 2.0 130 1.0 131 6.0 132 3.0 133 7.0 134 15.0 135 8.0 136 7.0 137 9.0 138 1.0 139 3.0 140 1.0 141 3.0 142 7.0 143 6.0 144 7.0 145 4.0 146 2.0 147 4.0 148 8.0 149 0.0 150 1437.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.3 #Duplication Level Percentage of deduplicated Percentage of total 1 67.04771371769384 33.725 2 19.980119284294233 20.1 3 6.113320079522863 9.225 4 2.286282306163022 4.6 5 0.7455268389662028 1.875 6 0.44731610337972166 1.35 7 0.5964214711729622 2.1 8 0.19880715705765406 0.8 9 0.2982107355864811 1.35 >10 2.0874751491053676 17.625 >50 0.19880715705765406 7.249999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTG 96 2.4 No Hit GTCGTAGATGGCCTCGTTATCTAGGAGAACAGCAACATCGGTGTGCTCAA 67 1.675 No Hit GCCTGTTCAAGTTGGTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGG 66 1.6500000000000001 No Hit CCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGC 61 1.525 No Hit GTCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAG 45 1.125 No Hit GTTCAAGTTGGTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGGCATA 45 1.125 No Hit CCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGCTTCAGGCGGTTGTGGA 30 0.75 No Hit CATATACTCGCTGTCTGGGAGCTTATTCAGCAGCAGTACGTAGCACAACA 26 0.65 No Hit GTGGTGCGACGCCTCTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGTG 23 0.575 No Hit GCCATCTTATTTTATCATCAAACATGCATCCCATTCCCAGGTAGCAAAAT 21 0.525 No Hit CTCTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTA 20 0.5 No Hit GCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCA 20 0.5 No Hit CCTCTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGT 20 0.5 No Hit GCCTCAGCATGGATGTCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCA 19 0.475 No Hit GTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGGCATATGTCGTAGAT 19 0.475 No Hit GCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGCTTCAGGCGG 19 0.475 No Hit GGCTTCTTGGTGGTGCGACGCCTCTTCTTCGCAGCTTCAGCGTGGATATC 18 0.44999999999999996 No Hit CTCAGCATGGATGTCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGG 18 0.44999999999999996 No Hit GCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGT 17 0.42500000000000004 No Hit GGTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGGCATATGTCGTAGA 16 0.4 No Hit CGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGC 16 0.4 No Hit CTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGCT 15 0.375 No Hit CTTCGCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAG 15 0.375 No Hit GTTGGTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGGCATATGTCGT 15 0.375 No Hit CCGGGAGTATGGCTTCTTGGTGGTGCGACGCCTCTTCTTCGCAGCTTCAG 15 0.375 No Hit CGCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTG 15 0.375 No Hit GCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAG 15 0.375 No Hit CTTTATTATTAATCTGACAATGAAATGAACAACACAAGCATATTATTCAC 14 0.35000000000000003 No Hit GCTTCTTGGTGGTGCGACGCCTCTTCTTCGCAGCTTCAGCGTGGATATCC 13 0.325 No Hit ATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAG 13 0.325 No Hit ATATTATTCACCAAAGCAACAGACGATTGGCCGCACAAGTTGCCATCTTA 13 0.325 No Hit CTCTACCAGTTTTTAACTGCCCATCTGTTTGTAGAACAGCTCGACCACGA 13 0.325 No Hit GGTGGTGCGACGCCTCTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGT 13 0.325 No Hit GGGAGTATGGCTTCTTGGTGGTGCGACGCCTCTTCTTCGCAGCTTCAGCG 13 0.325 No Hit CCGGCATATGTCGTAGATGGCCTCGTTATCTAGGAGAACAGCAACATCGG 13 0.325 No Hit GGCCGCACAAGTTGCCATCTTATTTTATCATCAAACATGCATCCCATTCC 12 0.3 No Hit CTCCGGCATATGTCGTAGATGGCCTCGTTATCTAGGAGAACAGCAACATC 11 0.27499999999999997 No Hit GTGCGACGCCTCTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGTGCTG 11 0.27499999999999997 No Hit GTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGCTTCAGGC 11 0.27499999999999997 No Hit CATGGATGTCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGC 11 0.27499999999999997 No Hit GGCATATGTCGTAGATGGCCTCGTTATCTAGGAGAACAGCAACATCGGTG 11 0.27499999999999997 No Hit ATATACTCGCTGTCTGGGAGCTTATTCAGCAGCAGTACGTAGCACAACAC 11 0.27499999999999997 No Hit AGCCTGTTCAAGTTGGTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCG 10 0.25 No Hit GGCTGCACAAGTTGCCATCTTATTTTATCATCAAACATGCATCCCATTCC 10 0.25 No Hit CACACGACAGGTAGCATCACGGACAAACACCTAATGGTAACCCTTAAACA 10 0.25 No Hit AGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAA 10 0.25 No Hit GCGACGCCTCTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCT 9 0.22499999999999998 No Hit CCTGTTCAAGTTGGTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGGC 9 0.22499999999999998 No Hit CCTCAGCATGGATGTCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAG 9 0.22499999999999998 No Hit CTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAG 9 0.22499999999999998 No Hit GTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCT 9 0.22499999999999998 No Hit CACCAAAGCAACAGACGATTGGCCGCACAAGTTGCCATCTTATTTTATCA 9 0.22499999999999998 No Hit TTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGCTT 8 0.2 No Hit CCATCTTATTTTATCATCAAACATGCATCCCATTCCCAGGTAGCAAAATA 8 0.2 No Hit AGCAAATTCACACACAGTGACAGTGATTAAAGGAACCACACAAGCTCAAG 8 0.2 No Hit GGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTT 8 0.2 No Hit ACGAGATCGACTGAAAGTCTTATTCTTGTCTTATGATAACTGACTGAAAC 7 0.17500000000000002 No Hit TGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGGCATATGTCGTAGATG 7 0.17500000000000002 No Hit ACCCAGGGTGCGTTCTGAGCAGCAGTCCTAGGATAGCTTCCATTATTGGC 7 0.17500000000000002 No Hit CCCATTTGCGACTAAAGTTTGATGTGTCTCAGCCAAACCAAGTTCCCATG 7 0.17500000000000002 No Hit GCCGCACAAGTTGCCATCTTATTTTATCATCAAACATGCATCCCATTCCC 7 0.17500000000000002 No Hit GCAGTTCATAACCTTTATTATTAATCTGACAATGAAATGAACAACACAAG 7 0.17500000000000002 No Hit CTTCTTCGCAGCTTCAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACA 7 0.17500000000000002 No Hit CCAGTTTTTAACTGCCCATCTGTTTGTAGAACAGCTCGACCACGAAGCCC 7 0.17500000000000002 No Hit ACCGGGAGTATGGCTTCTTGGTGGTGCGACGCCTCTTCTTCGCAGCTTCA 7 0.17500000000000002 No Hit GTACATAGCTGTCCAGGTAAGCTTTGCAGGCTTCAGGCGGTTGTGGAAGT 7 0.17500000000000002 No Hit CGGGAGTATGGCTTCTTGGTGGTGCGACGCCTCTTCTTCGCAGCTTCAGC 7 0.17500000000000002 No Hit GTTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGCTTCAGG 7 0.17500000000000002 No Hit CCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGG 6 0.15 No Hit GTAGGTTGGCCTCTCGATGTCAAGAGACCTCCGGCATATGTCGTAGATGG 6 0.15 No Hit GTGGCTTCTTGGTGGTGCGACGCCTCTTCTTCGCAGCTTCAGCGTGGATA 6 0.15 No Hit CACACATTGTTGGCCGAATTTGTACCCTCCTGCGGATGCTTCAAAGGCCG 6 0.15 No Hit AGCGGGTACACCTCGGGCCGGACCCATTTGCTGGCGGCCATCGGAGTCGC 6 0.15 No Hit AGCATGGATGTCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAA 6 0.15 No Hit CTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTAAGCTTTGCAGGCTTCA 6 0.15 No Hit AGCACAGTTCACTGTGGCTGCCACTGGTAGACCCAGTGACATGCGGAACT 6 0.15 No Hit CCCATCTGTTTGTAGAACAGCTCGACCACGAAGCCCATTTGCGACTAAAG 6 0.15 No Hit CGGCATATGTCGTAGATGGCCTCGTTATCTAGGAGAACAGCAACATCGGT 5 0.125 No Hit CAGCGTGGATATCCTTCTTGTGCTGCTTCCTGTACATAGCTGTCCAGGTA 5 0.125 No Hit ATTCACCAAAGCAACAGACGATTGGCCGCACAAGTTGCCATCTTATTTTA 5 0.125 No Hit ACCTGAGTAACCAATATCGTATGAAATCCTTCCATCCGCATTGAACAGCC 5 0.125 No Hit ATACCGTTCCCCGCCGCGCCGCCATGGCCGCCGCCGCCGCCGTCACCGGC 5 0.125 No Hit ATACGGGTGAAGGAAGTGTTCAAATAGCCAAGCACGGAGAATTGATACTG 5 0.125 No Hit ACCTGGGGTTCCCCCTTGAGCTTCCAGAGCCGGGTGCCGCAGTCGTAGAC 5 0.125 No Hit CACAGATCTCGAACAGAGACGACGAAATTTCCTCTCCCTCAGAACGGGTT 5 0.125 No Hit CAACAATGGTAGAATGTCGGAGCAACACCCTACAGGACATGAAACACAGA 5 0.125 No Hit CCCAGGGTGCGTTCTGAGCAGCAGTCCTAGGATAGCTTCCATTATTGGCT 5 0.125 No Hit GGTTGGCCTCTCGATGTCAAGAGACCTCCGGCATATGTCGTAGATGGCCT 5 0.125 No Hit GGCCTGTTCAAGTTGGTGTAGGTTGGCCTCTCGATGTCAAGAGACCTCCG 5 0.125 No Hit CACACACTGACTTGGAGCAGAGCGGGCATGAAAACTGGCAATGTTCCTCC 5 0.125 No Hit ACCGGATATGGCCCTTCAGCTTGTACTCCTCTATGAGACTGTACATCCTC 5 0.125 No Hit ACGAAATATTACATGTATCTAGACGCTTTTTGGATATAGATACATCAAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR5262810 read2 length is 90-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262810_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 90-150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.43 37.0 37.0 37.0 37.0 37.0 2 36.192 37.0 37.0 37.0 37.0 37.0 3 36.244 37.0 37.0 37.0 37.0 37.0 4 36.357 37.0 37.0 37.0 37.0 37.0 5 36.414 37.0 37.0 37.0 37.0 37.0 6 36.3545 37.0 37.0 37.0 37.0 37.0 7 36.3125 37.0 37.0 37.0 37.0 37.0 8 36.513 37.0 37.0 37.0 37.0 37.0 9 36.4225 37.0 37.0 37.0 37.0 37.0 10-14 36.439099999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.454600000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.4216 37.0 37.0 37.0 37.0 37.0 25-29 36.4109 37.0 37.0 37.0 37.0 37.0 30-34 36.302200000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.3703 37.0 37.0 37.0 37.0 37.0 40-44 36.3959 37.0 37.0 37.0 37.0 37.0 45-49 36.2939 37.0 37.0 37.0 37.0 37.0 50-54 36.315200000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.2611 37.0 37.0 37.0 37.0 37.0 60-64 36.263600000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.2899 37.0 37.0 37.0 37.0 37.0 70-74 36.2184 37.0 37.0 37.0 37.0 37.0 75-79 36.207100000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.173 37.0 37.0 37.0 37.0 37.0 85-89 36.2232 37.0 37.0 37.0 37.0 37.0 90-94 36.194638359589895 37.0 37.0 37.0 37.0 37.0 95-99 36.15254595023306 37.0 37.0 37.0 37.0 37.0 100-104 36.0378326287258 37.0 37.0 37.0 37.0 37.0 105-109 35.99800459476715 37.0 37.0 37.0 37.0 37.0 110-114 35.88873104200083 37.0 37.0 37.0 37.0 37.0 115-119 35.839650207271994 37.0 37.0 37.0 37.0 37.0 120-124 35.885444920530226 37.0 37.0 37.0 37.0 37.0 125-129 35.88244441923506 37.0 37.0 37.0 37.0 37.0 130-134 35.86933138003531 37.0 37.0 37.0 37.0 37.0 135-139 35.94933124995205 37.0 37.0 37.0 37.0 37.0 140-144 35.798000674077 37.0 37.0 37.0 37.0 37.0 145-149 35.7583937348611 37.0 37.0 37.0 37.0 37.0 150 35.92672714584787 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 1.0 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 4.0 22 3.0 23 7.0 24 7.0 25 7.0 26 3.0 27 2.0 28 12.0 29 9.0 30 12.0 31 20.0 32 33.0 33 55.0 34 117.0 35 337.0 36 2661.0 37 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.325 21.25 6.35 25.074999999999996 2 28.225 21.5 26.950000000000003 23.325000000000003 3 20.625 23.075000000000003 33.15 23.150000000000002 4 25.95 27.975 23.575 22.5 5 26.775 29.799999999999997 19.45 23.974999999999998 6 22.05 30.525000000000002 22.95 24.474999999999998 7 22.525000000000002 18.775 35.9 22.8 8 22.1 20.175 26.775 30.95 9 23.375 20.875 29.025000000000002 26.724999999999998 10-14 25.5 23.16 25.46 25.88 15-19 24.205 22.67 27.445000000000004 25.679999999999996 20-24 25.865 24.125 27.365000000000002 22.645 25-29 26.365 24.02 27.07 22.545 30-34 25.19 24.88 28.189999999999998 21.740000000000002 35-39 26.450000000000003 23.625 27.52 22.405 40-44 25.259999999999998 24.18 27.994999999999997 22.564999999999998 45-49 25.585 24.23 27.875 22.31 50-54 26.35 24.68 25.71 23.26 55-59 26.355 25.335 26.179999999999996 22.13 60-64 25.255 24.654999999999998 28.21 21.88 65-69 25.39 24.255 27.175 23.18 70-74 26.174999999999997 24.42 27.375 22.03 75-79 25.085 23.880000000000003 27.785 23.25 80-84 25.790000000000003 23.455000000000002 27.63 23.125 85-89 25.655 23.955000000000002 27.215 23.175 90-94 25.08001600320064 23.779755951190236 27.465493098619724 23.6747349469894 95-99 25.17636463701406 24.94121178766198 26.56226547255716 23.3201581027668 100-104 24.351916725052547 22.780502452206985 32.38915023521169 20.478430587528777 105-109 24.10420904396808 20.6618682522297 34.16523235800344 21.068690345798778 110-114 25.425652667423382 28.490351872871738 23.55908689620381 22.52490856350107 115-119 25.383931971062317 27.211575072978807 25.726615052671658 21.677877903287218 120-124 23.686907507045863 25.07045862157315 27.965667435306173 23.276966436074815 125-129 22.20931733126855 27.30674925796877 26.05497483546264 24.42895857530004 130-134 23.14222280266423 24.121718688781506 28.470680423142227 24.26537808541204 135-139 23.12952534191472 24.255832662912308 25.89165996245642 26.722982032716548 140-144 22.250917493543565 25.146119342123146 28.625798559195324 23.97716460513796 145-149 20.41551246537396 25.526315789473685 26.82825484764543 27.22991689750692 150 19.469644103279833 28.401953942777393 28.401953942777393 23.726448011165388 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.5 21 0.5 22 0.5 23 0.5 24 1.0 25 1.5 26 1.0 27 1.0 28 1.0 29 2.0 30 5.5 31 11.0 32 14.0 33 11.0 34 23.0 35 32.5 36 37.5 37 53.0 38 69.5 39 79.5 40 96.5 41 127.5 42 149.0 43 140.5 44 154.5 45 204.0 46 246.0 47 271.5 48 268.0 49 286.0 50 281.5 51 213.0 52 151.5 53 119.0 54 124.5 55 102.5 56 73.0 57 87.0 58 77.5 59 52.5 60 41.0 61 41.5 62 42.5 63 36.0 64 27.0 65 24.5 66 31.5 67 35.0 68 29.5 69 25.5 70 24.5 71 19.0 72 13.5 73 15.0 74 10.5 75 4.0 76 2.5 77 1.5 78 0.5 79 0.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 90-91 1.0 92-93 0.0 94-95 1.0 96-97 1.0 98-99 0.0 100-101 1.0 102-103 0.0 104-105 0.0 106-107 2398.0 108-109 6.0 110-111 8.0 112-113 2.0 114-115 3.0 116-117 6.0 118-119 9.0 120-121 2.0 122-123 6.0 124-125 4.0 126-127 3.0 128-129 10.0 130-131 8.0 132-133 10.0 134-135 23.0 136-137 16.0 138-139 4.0 140-141 4.0 142-143 13.0 144-145 14.0 146-147 6.0 148-149 8.0 150-151 1433.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.49999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 66.73913043478261 38.375 2 19.608695652173914 22.55 3 6.739130434782608 11.625 4 2.4347826086956523 5.6000000000000005 5 1.0434782608695654 3.0 6 1.2173913043478262 4.2 7 0.47826086956521735 1.925 8 0.5217391304347827 2.4 9 0.17391304347826086 0.8999999999999999 >10 1.0434782608695654 9.425 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTG 35 0.8750000000000001 No Hit GCCGCCGCGCCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCT 35 0.8750000000000001 No Hit GCCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGC 28 0.7000000000000001 No Hit CCGCGCCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGG 21 0.525 No Hit GTTTTCTAAGGCTGCCAAGATGGTTCTGAAGACTGAGCTTTGCCGTTTCA 21 0.525 No Hit GCCGCGCCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAG 20 0.5 No Hit GGTTCATTCGTGCTGATTCACAGGTTTTCCTTTTTGCCAACTCAAAATGC 18 0.44999999999999996 No Hit CCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGCCAAGATGGTTCT 16 0.4 No Hit AGATGGTTCTGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATAC 15 0.375 No Hit CTGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATACCCAGGAAA 15 0.375 No Hit GATGGTTCTGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATACC 14 0.35000000000000003 No Hit GGTTTTCTAAGGCTGCCAAGATGGTTCTGAAGACTGAGCTTTGCCGTTTC 13 0.325 No Hit GTTTCAGCGGCCAGAAGATATACCCAGGAAAGGGTATAAGGTTCATTCGT 12 0.3 No Hit GTTCATTCGTGCTGATTCACAGGTTTTCCTTTTTGCCAACTCAAAATGCA 11 0.27499999999999997 No Hit TCTAAGGCTGCCAAGATGGTTCTGAAGACTGAGCTTTGCCGTTTCAGCGG 11 0.27499999999999997 No Hit GGAACTTGTGAAGGTTTTCTAAGGCTGCCAAGATGGTTCTGAAGACTGAG 11 0.27499999999999997 No Hit GGAACTTCTGAAGGTGTTCTAAGGCTGCCAAGATGGTTCTGAAGACTGAG 11 0.27499999999999997 No Hit GTTCTGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATACCCAGG 10 0.25 No Hit GCGGCCAGAAGATATACCCAGGAAAGGGTATAAGGTTCATTCGTGCTGAT 10 0.25 No Hit ACTCAAAATGCAAGCGCTACTTCCACAACCGCCTGAAGCCTGCAAAGCTT 10 0.25 No Hit ATTACACTGTTGGAAAGGAGATTGTAGATCTATGCCTGGACCGTGTGCGC 10 0.25 No Hit GTGATGTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGT 10 0.25 No Hit GCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGCCAA 10 0.25 No Hit AGAAGATATACCCAGGAAAGGGTATAAGGTTCATTCGTGCTGATTCACAG 10 0.25 No Hit CCTTGCTCAAGGAACTTCTGAAGGTGTTCTAAGGCTGCCAAGATGGTTCT 9 0.22499999999999998 No Hit GCTCGAATCAGTGTCAAGTTAGTATCTGAGGCTGGTGTAGGAGTTGTTGC 9 0.22499999999999998 No Hit TGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATACCCAGGAAAG 9 0.22499999999999998 No Hit GGCAAGAAGTCTAAGCTTGGTTTCACCATTTATCCTTCTCCACAGGTCTC 9 0.22499999999999998 No Hit GTAGATCTATGCCTGGACCGTGTGCGCAAGTTGGCAGACAATTGCACTGG 8 0.2 No Hit GTGATATACACGATCTTACCGCACATACAGATCTGAGCTATTTTCATTTG 8 0.2 No Hit GCCGCCGCCCCTCCTCCTTGCTCAAGGAACTTCTGAAGGTGTTCTAAGGC 8 0.2 No Hit GCCACTGATCAAGGCTTTGCAACAGGTCCTCGCTAATAGCTTGCGCTATT 8 0.2 No Hit GCTCATCTCAGGGAAGGAGGATGCCGCTAACAACTTTGCCCGTGGACATT 8 0.2 No Hit CCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGCC 8 0.2 No Hit GCGCCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCT 8 0.2 No Hit AGAAGATTTACCCAGGGAAGGGTATCAGGTTCATTCGTGCGGATTCGCAG 8 0.2 No Hit CCTCCTTGCTCAAGGAACTTCTGAAGGTGTTCTAAGGCTGCCAAGATGGT 8 0.2 No Hit GGTGTTCAATGCTGTCGGTGGTGGAACTGGATCAGGACTTGGTTCTCTGT 8 0.2 No Hit GTGTGCGCAAGTTGGCAGACAATTGCACTGGGCTGCAGGGATTCTTGGTG 8 0.2 No Hit ACTACAGTGGCTATGCTATCACCCTGGCTTGATGGTAGATCATCGATCAG 8 0.2 No Hit CATGCATGCAGATCAGGTCACATGGATGCAAGACTAGTAGTACCAACTAG 7 0.17500000000000002 No Hit ATAAGGTTCATTCGTGCTGATTCACAGGTTTTCCTTTTTGCCAACTCAAA 7 0.17500000000000002 No Hit GCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGCCAAGATGGTTCTGAAG 7 0.17500000000000002 No Hit GTGGAACTGGATCAGGACTTGGTTCTCTGTTGTTGGAGCGTCTCTCAGTG 7 0.17500000000000002 No Hit AAGATGGTTCTGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATA 7 0.17500000000000002 No Hit CAGGTTTTCCTTTTTGCCAACTCAAAATGCAAGCGCTACTTCCACAACCG 7 0.17500000000000002 No Hit CCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGCCAAGAT 7 0.17500000000000002 No Hit ATCACCTCCTGGTTCCAAGATGTCGAAACGAGGGCGCGGAGGTTCCGCTG 7 0.17500000000000002 No Hit CTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGCCAAGATGGTT 7 0.17500000000000002 No Hit CGACAACAGAGGATGCCAGTTTCTAGATGGATGGTTACAAATCGCACGTC 7 0.17500000000000002 No Hit GAAGTCTAAGCTTGGTTTCACCATTTATCCTTCTCCACAGGTCTCAACAG 7 0.17500000000000002 No Hit GTGAAGGTTTTCTAAGGCTGCCAAGATGGTTCTGAAGACTGAGCTTTGCC 6 0.15 No Hit GCCGCCCCTCCTCCTTGCTCAAGGAACTTCTGAAGGTGTTCTAAGGCTGC 6 0.15 No Hit CTTTGCAACAGGTCCTCGCTAATAGCTTGCGCTATTTCTTGAGGAGGCCG 6 0.15 No Hit AGGAGATTGTAGATCTATGCCTGGACCGTGTGCGCAAGTTGGCAGACAAT 6 0.15 No Hit GTTGGCAGACAATTGCACTGGGCTGCAGGGATTCTTGGTGTTCAATGCTG 6 0.15 No Hit GTCTAAGCTTGGTTTCACCATTTATCCTTCTCCACAGGTCTCAACAGCTG 6 0.15 No Hit CTTGGTTCTCTGTTGTTGGAGCGTCTCTCAGTGGATTATGGCAAGAAGTC 6 0.15 No Hit GTCAGCTCTACAGTAACAGAATTTCAGGTTCATCAATCTCAGCAAAATCA 6 0.15 No Hit AAAGGGTATAAGGTTCATTCGTGCTGATTCACAGGTTTTCCTTTTTGCCA 6 0.15 No Hit CTCAGTGGATTATGGCAAGAAGTCTAAGCTTGGTTTCACCATTTATCCTT 6 0.15 No Hit GCTTGGTTTCACCATTTATCCTTCTCCACAGGTCTCAACAGCTGTCGTAG 6 0.15 No Hit CGCGCCGCCGCCCCTCCTCCTTGCTCAAGGAACTTCTGAAGGTGTTCTAA 6 0.15 No Hit GGGATTCTTGGTGTTCAATGCTGTCGGTGGTGGAACTGGATCAGGACTTG 6 0.15 No Hit GTTTTCCTTTTTGCCAACTCAAAATGCAAGCGCTACTTCCACAACCGCCT 6 0.15 No Hit GGGTATAAGGTTCATTCGTGCTGATTCACAGGTTTTCCTTTTTGCCAACT 6 0.15 No Hit AGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATACCCAGGAAAGGGT 6 0.15 No Hit CTTTTTGCCAACTCAAAATGCAAGCGCTACTTCCACAACCGCCTGAAGCC 6 0.15 No Hit GCGCAAGTTGGCAGACAATTGCACTGGGCTGCAGGGATTCTTGGTGTTCA 6 0.15 No Hit GGGCAAGCCACTGATCAAGGCTTTGCAACAGGTCCTCGCTAATAGCTTGC 6 0.15 No Hit GTAAAAGGACATGCGGACCATGTACTTATTTCTGGCCATGATGGCGGCAC 6 0.15 No Hit CTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGCTGCCAAG 6 0.15 No Hit GCCAAGATGGTTCTGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGAT 6 0.15 No Hit CCTCTCAGCTCTCCTCTCTCTCTTTCTCCGCCGCCGCAGTCTCCGAGGAA 6 0.15 No Hit CAAGATGGTTCTGAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATAT 6 0.15 No Hit CTTGAAATATGCATAATTCCCAAAAGGTGATATACACGATCTTACCGCAC 6 0.15 No Hit GAACTTGTGAAGGTTTTCTAAGGCTGCCAAGATGGTTCTGAAGACTGAGC 6 0.15 No Hit GTATAAGGTTCATTCGTGCTGATTCACAGGTTTTCCTTTTTGCCAACTCA 6 0.15 No Hit GAAGATATACCCAGGAAAGGGTATAAGGTTCATTCGTGCTGATTCACAGG 6 0.15 No Hit GTATGTATCCCTTGTACTCCTTCCGTCCCATATTAAGTTACTCAAATTTG 5 0.125 No Hit CACAGGTTTTCCTTTTTGCCAACTCAAAATGCAAGCGCTACTTCCACAAC 5 0.125 No Hit CTCGACCCAATCCTCTCGCCGCCGCCGTAGCAACCTTTGAGAGAACGAGA 5 0.125 No Hit ATCCGCGCCGCCGCCCCTCCTCCTTGCTCAAGGAACTTCTGAAGGTGTTC 5 0.125 No Hit TATGCCTGGACCGTGTGCGCAAGTTGGCAGACAATTGCACTGGGCTGCAG 5 0.125 No Hit TGGAACTGGATCAGGACTTGGTTCTCTGTTGTTGGAGCGTCTCTCAGTGG 5 0.125 No Hit GTATGGTATGTTACAGCAAAATGCTTGGAGGCCACAGAGGGGGCTTCCTG 5 0.125 No Hit CAGCATTCCTATCCCCAGAGTGTGGAGGTTGCGATTGTCTGACCATGCCC 5 0.125 No Hit AGACAATTGCACTGGGCTGCAGGGATTCTTGGTGTTCAATGCTGTCGGTG 5 0.125 No Hit GTTGTTGGAGCGTCTCTCAGTGGATTATGGCAAGAAGTCTAAGCTTGGTT 5 0.125 No Hit GAAGACTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATACCCAGGAAAGG 5 0.125 No Hit CTTTGCGTTATTTAATGAGAACTCGAAGCCTGGACCAAGTTCTGAGAAGC 5 0.125 No Hit CTGAGCTTTGCCGTTTCAGCGGCCAGAAGATATACCCAGGAAAGGGTATA 5 0.125 No Hit CACGCACCACACCAAGAAGCATCCCCTCCCCAAGAAAGAAAAATCTCCCC 5 0.125 No Hit CGCGCCGCTCCTCCTCCTTGCTTAAGGAACTTGTGAAGGTTTTCTAAGGC 5 0.125 No Hit GATGTATCTGTCTTGCCGTGCGGTCACACGATTCATGAAAACTGCTTGAA 5 0.125 No Hit AGGTTTTCTAAGGCTGCCAAGATGGTTCTGAAGACTGAGCTTTGCCGTTT 5 0.125 No Hit GGCTTTGCAACAGGTCCTCGCTAATAGCTTGCGCTATTTCTTGAGGAGGC 5 0.125 No Hit GCGAAGGTGGTGAGCTTCCAGGTCACAAGGTTATTGGTGATATTGCAGTT 5 0.125 No Hit CCGACCCTTACGCGCGCGCGCAGGCCAGGTTCTGGGCCGACTACGTCGAC 5 0.125 No Hit GATATACCCAGGAAAGGGTATAAGGTTCATTCGTGCTGATTCACAGGTTT 5 0.125 No Hit CACCTCTTCGGCTAAGCGACGTCGCTAGACCCGCCGATCGATACGCCACG 5 0.125 No Hit GTTGCTGGTGACCATGGCAAGGAGTCCAAGGATAGGGAGGAGCAGGCTGA 5 0.125 No Hit GGCAGACAATTGCACTGGGCTGCAGGGATTCTTGGTGTTCAATGCTGTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATATC 25 0.009057255 128.97931 140-144 >>END_MODULE Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593972 spots for ERR5262810.sra Written 1593972 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra Read 1593967 spots for ERR5262810.sra Written 1593967 spots for ERR5262810.sra SRR ids: ['ERR5262810.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4wijfn0i ERR5262810.sra spots: 31879345 blocks: [[1, 1593967], [1593968, 3187934], [3187935, 4781901], [4781902, 6375868], [6375869, 7969835], [7969836, 9563802], [9563803, 11157769], [11157770, 12751736], [12751737, 14345703], [14345704, 15939670], [15939671, 17533637], [17533638, 19127604], [19127605, 20721571], [20721572, 22315538], [22315539, 23909505], [23909506, 25503472], [25503473, 27097439], [27097440, 28691406], [28691407, 30285373], [30285374, 31879345]] ERR5262810 file size 10461562 ERR5262810 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262810 ERR5262810_1.fastq ERR5262810_2.fastq Input file: ERR5262810_1.fastq Paired file: ERR5262810_2.fastq trimmed: ERR5262810-trimmed-pair1.fastq, ERR5262810-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:23:41 2024 >> started Fri Dec 6 12:24:17 2024 >> done (35.462s) 31879345 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 31879344 (100.00%) read pairs available; of these: 9086 ( 0.03%) trimmed read pairs available after processing 31870258 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 1 0.00% 29 0 0.00% 30 2 0.00% 31 2 0.00% 32 3 0.00% 33 1 0.00% 34 3 0.00% 35 1 0.00% 36 0 0.00% 37 1 0.00% 38 2 0.00% 39 0 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 0 0.00% 44 0 0.00% 45 1 0.00% 46 3 0.00% 47 2 0.00% 48 2 0.00% 49 323 0.00% 50 365 0.00% 51 408 0.00% 52 419 0.00% 53 424 0.00% 54 507 0.00% 55 602 0.00% 56 629 0.00% 57 716 0.00% 58 830 0.00% 59 974 0.00% 60 1140 0.00% 61 1219 0.00% 62 1380 0.00% 63 1554 0.00% 64 1715 0.01% 65 1934 0.01% 66 2125 0.01% 67 2319 0.01% 68 2619 0.01% 69 3115 0.01% 70 3561 0.01% 71 4033 0.01% 72 4717 0.01% 73 5212 0.02% 74 5818 0.02% 75 6413 0.02% 76 7034 0.02% 77 7503 0.02% 78 8478 0.03% 79 9354 0.03% 80 10575 0.03% 81 11830 0.04% 82 13584 0.04% 83 14654 0.05% 84 16114 0.05% 85 17221 0.05% 86 18464 0.06% 87 19589 0.06% 88 21095 0.07% 89 22279 0.07% 90 24296 0.08% 91 26538 0.08% 92 28343 0.09% 93 30900 0.10% 94 33142 0.10% 95 34380 0.11% 96 36216 0.11% 97 37339 0.12% 98 39025 0.12% 99 40781 0.13% 100 42895 0.13% 101 44754 0.14% 102 47442 0.15% 103 49590 0.16% 104 51427 0.16% 105 54608 0.17% 106 56036 0.18% 107 57459 0.18% 108 59255 0.19% 109 60155 0.19% 110 62897 0.20% 111 64854 0.20% 112 67312 0.21% 113 70229 0.22% 114 73361 0.23% 115 74741 0.23% 116 77102 0.24% 117 78312 0.25% 118 80378 0.25% 119 80655 0.25% 120 81345 0.26% 121 83740 0.26% 122 85662 0.27% 123 88932 0.28% 124 92180 0.29% 125 93738 0.29% 126 94736 0.30% 127 96383 0.30% 128 97075 0.30% 129 99761 0.31% 130 99349 0.31% 131 100851 0.32% 132 103226 0.32% 133 104747 0.33% 134 107386 0.34% 135 109140 0.34% 136 110786 0.35% 137 111599 0.35% 138 113140 0.35% 139 116876 0.37% 140 117731 0.37% 141 123237 0.39% 142 127289 0.40% 143 127960 0.40% 144 131183 0.41% 145 128989 0.40% 146 132972 0.42% 147 258263 0.81% 148 120463 0.38% 149 119488 0.37% 150 26663824 83.64% 31879344 reads passed initial QC criterion=sequence-density sequence-density=2.29 sequence-density-rank=1 fanout-score=2.60 fanout-score-rank=27 prefix-density=2.59 prefix-fanout=2.3 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=806.26 fanout-score-rank=1 prefix-density=5.20 prefix-fanout=1.0 sequence=GGCGTCGCGGCACCGCCCGCGTTCAGCTTGTTGAAGGCCATGCCGGAGCCCTGGCCGGCGAAGACGAAGATGAGCGTGGAGATGAACTCGGCGACGGCCGCCTTGAGGGCGCCGGCCTGGTACACCTCATGGTGGCTCCCCACGGCAACCCTGCTGATCGGCATTCTGACCACCAAAAGCTAGAGATCGAGACCGGCCGGAGCTGGGAGAGATGATGAGAGAGTGTT criterion=sequence-density sequence-density=8.32 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=30 prefix-density=8.29 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.26 sequence-density-rank=14 fanout-score=32.16 fanout-score-rank=1 prefix-density=1.05 prefix-fanout=7.8 sequence=CGGCGGCGGCGGAGCTGACGGGCAGCCACCTGGACGAGGTGAAGCGGATGGTGGCCCAGTTCCGGGAGCCCGTGGTGAAGATCGAGGGGGCCAGCCTCCGCGTGGGCCAGGTGGCCGCCGTGGCCCAGGCAAA Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262810 ERR5262810_1.fastq ERR5262810_2.fastq Input file: ERR5262810_1.fastq Paired file: ERR5262810_2.fastq trimmed: ERR5262810-trimmed-pair1.fastq, ERR5262810-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:27:17 2024 >> started Fri Dec 6 12:27:40 2024 >> done (23.024s) 21252896 read pairs processed; of these: 91 ( 0.00%) short read pairs filtered out after trimming by size control 264 ( 0.00%) empty read pairs filtered out after trimming by size control 21252541 (100.00%) read pairs available; of these: 137 ( 0.00%) trimmed read pairs available after processing 21252404 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 1 0.00% 29 0 0.00% 30 1 0.00% 31 1 0.00% 32 1 0.00% 33 0 0.00% 34 2 0.00% 35 0 0.00% 36 0 0.00% 37 1 0.00% 38 1 0.00% 39 0 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 0 0.00% 44 0 0.00% 45 1 0.00% 46 3 0.00% 47 1 0.00% 48 2 0.00% 49 287 0.00% 50 337 0.00% 51 174 0.00% 52 153 0.00% 53 62 0.00% 54 87 0.00% 55 93 0.00% 56 353 0.00% 57 673 0.00% 58 771 0.00% 59 923 0.00% 60 1086 0.01% 61 533 0.00% 62 164 0.00% 63 472 0.00% 64 699 0.00% 65 1855 0.01% 66 1690 0.01% 67 684 0.00% 68 2513 0.01% 69 2253 0.01% 70 1603 0.01% 71 3274 0.02% 72 3305 0.02% 73 3373 0.02% 74 4138 0.02% 75 5553 0.03% 76 4893 0.02% 77 4446 0.02% 78 5607 0.03% 79 4716 0.02% 80 7027 0.03% 81 9319 0.04% 82 9740 0.05% 83 9529 0.04% 84 9856 0.05% 85 10209 0.05% 86 14012 0.07% 87 12643 0.06% 88 13491 0.06% 89 15442 0.07% 90 16522 0.08% 91 16426 0.08% 92 19716 0.09% 93 20750 0.10% 94 22414 0.11% 95 22743 0.11% 96 24340 0.11% 97 24886 0.12% 98 25964 0.12% 99 27262 0.13% 100 29542 0.14% 101 30121 0.14% 102 30217 0.14% 103 34874 0.16% 104 33273 0.16% 105 35785 0.17% 106 39439 0.19% 107 38181 0.18% 108 38889 0.18% 109 40008 0.19% 110 42680 0.20% 111 43036 0.20% 112 44196 0.21% 113 47599 0.22% 114 47689 0.22% 115 49896 0.23% 116 52200 0.25% 117 51454 0.24% 118 53313 0.25% 119 53337 0.25% 120 53672 0.25% 121 56354 0.27% 122 56680 0.27% 123 59458 0.28% 124 61199 0.29% 125 62644 0.29% 126 63188 0.30% 127 64480 0.30% 128 64722 0.30% 129 66103 0.31% 130 66631 0.31% 131 67179 0.32% 132 69186 0.33% 133 69788 0.33% 134 71485 0.34% 135 72893 0.34% 136 73860 0.35% 137 74291 0.35% 138 75830 0.36% 139 77678 0.37% 140 78942 0.37% 141 83166 0.39% 142 85205 0.40% 143 85110 0.40% 144 88214 0.42% 145 86646 0.41% 146 89106 0.42% 147 171801 0.81% 148 80565 0.38% 149 79616 0.37% 150 17772042 83.62% criterion=sequence-density sequence-density=0.13 sequence-density-rank=1 fanout-score=6.30 fanout-score-rank=37 prefix-density=0.21 prefix-fanout=4.0 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.03 sequence-density-rank=37 fanout-score=361.20 fanout-score-rank=1 prefix-density=0.63 prefix-fanout=16.3 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTG criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=37 prefix-density=0.59 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=12 fanout-score=175.13 fanout-score-rank=1 prefix-density=0.97 prefix-fanout=19.6 sequence=GCCGCCGCCGTC ERR5262810 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:28:40 Started mapping on | Dec 06 12:28:40 Finished on | Dec 06 12:31:33 Mapping speed, Million of reads per hour | 663.38 Number of input reads | 31878989 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 30684943 Uniquely mapped reads % | 96.25% Average mapped length | 290.71 Number of splices: Total | 29240197 Number of splices: Annotated (sjdb) | 27292549 Number of splices: GT/AG | 28827119 Number of splices: GC/AG | 345932 Number of splices: AT/AC | 19174 Number of splices: Non-canonical | 47972 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.45 Insertion rate per base | 0.01% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 353511 % of reads mapped to multiple loci | 1.11% Number of reads mapped to too many loci | 4697 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.61% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 840535 840535 840535 N_multimapping 353511 353511 353511 N_noFeature 1205143 29888655 1452516 N_ambiguous 650141 3995 101878 UnstrandedReadsAssigned:28829659 PositiveStrandReadsAssigned:792293 NegativeStrandReadsAssigned:29130549 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262810 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262810-trimmed-pair1.fastq ERR5262810-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,878,989 reads, 29,576,171 reads pseudoaligned [quant] estimated average fragment length: 263.837 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,253 rounds 52973 ERR5262810.ke.tsv 35125 ERR5262810.se.tsv 88098 total ==> ERR5262810.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 674.012 0 0 PNS24247 1044 781.163 160.552 10.1142 PNS24249 1928 1665.16 325.255 9.61224 PNS24246 1044 781.163 160.552 10.1142 PNS24248 1044 781.163 160.552 10.1142 PNS24244 1471 1208.16 168.088 6.84652 PNS24243 293 104.843 2 0.938751 KQK14069 1603 1340.16 35396.9 1299.77 KQK14071 474 241.528 189.489 38.6077 ==> ERR5262810.se.tsv <== BRADI_1g14170v3 36115 BRADI_1g53295v3 291 BRADI_1g59795v3 560 BRADI_1g07683v3 0 BRADI_1g00485v3 57 BRADI_1g20270v3 1340 BRADI_1g74790v3 1538 BRADI_1g09890v3 0 BRADI_1g77505v3 282 BRADI_1g48960v3 0 ERR5262810 completed mapping pipeline successfully