Starting /dee2/code/volunteer_pipeline.sh ERR5262811 current disk space = 1550673711104 free memory = 1604039188 ERR5262811 SRAfilesize 806d0b76d29e73e95044bd2a6433b2cb ERR5262811.sra ERR5262811.sra file validated ERR5262811 is paired end ERR5262811 is conventional basespace ERR5262811 read1 length is 60-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262811_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 60-150 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.44375 37.0 37.0 37.0 37.0 37.0 2 36.342 37.0 37.0 37.0 37.0 37.0 3 36.593 37.0 37.0 37.0 37.0 37.0 4 36.5755 37.0 37.0 37.0 37.0 37.0 5 36.661 37.0 37.0 37.0 37.0 37.0 6 36.657 37.0 37.0 37.0 37.0 37.0 7 36.4825 37.0 37.0 37.0 37.0 37.0 8 36.519 37.0 37.0 37.0 37.0 37.0 9 36.506 37.0 37.0 37.0 37.0 37.0 10-14 36.5259 37.0 37.0 37.0 37.0 37.0 15-19 36.4627 37.0 37.0 37.0 37.0 37.0 20-24 36.394999999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.3524 37.0 37.0 37.0 37.0 37.0 30-34 36.299400000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.2219 37.0 37.0 37.0 37.0 37.0 40-44 36.11 37.0 37.0 37.0 37.0 37.0 45-49 36.1109 37.0 37.0 37.0 37.0 37.0 50-54 36.1573 37.0 37.0 37.0 37.0 37.0 55-59 36.1306 37.0 37.0 37.0 37.0 37.0 60-64 36.07701875468867 37.0 37.0 37.0 37.0 37.0 65-69 36.04116029007252 37.0 37.0 37.0 37.0 37.0 70-74 36.03236271799316 37.0 37.0 37.0 37.0 37.0 75-79 35.896248124062026 37.0 37.0 37.0 37.0 37.0 80-84 35.932089330686736 37.0 37.0 37.0 37.0 37.0 85-89 35.977992008660316 37.0 37.0 37.0 37.0 37.0 90-94 35.851193507160765 37.0 37.0 37.0 37.0 37.0 95-99 35.867646725687585 37.0 37.0 37.0 37.0 37.0 100-104 35.661865481787444 37.0 37.0 37.0 37.0 37.0 105-109 35.715044135370015 37.0 37.0 37.0 37.0 37.0 110-114 35.68592247635126 37.0 37.0 37.0 37.0 37.0 115-119 35.7316584833192 37.0 37.0 37.0 37.0 37.0 120-124 35.59270958925806 37.0 37.0 37.0 37.0 37.0 125-129 35.54644168225239 37.0 37.0 37.0 37.0 37.0 130-134 35.564249297147015 37.0 37.0 37.0 37.0 37.0 135-139 35.464866396866356 37.0 37.0 37.0 37.0 37.0 140-144 35.463497168440384 37.0 37.0 37.0 37.0 37.0 145-149 35.396375062935405 37.0 37.0 37.0 37.0 37.0 150 35.455688965122924 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 3.0 21 1.0 22 3.0 23 4.0 24 10.0 25 12.0 26 13.0 27 14.0 28 19.0 29 23.0 30 31.0 31 46.0 32 49.0 33 100.0 34 139.0 35 446.0 36 2795.0 37 291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.0847711927982 12.10302575643911 9.127281820455114 39.68492123030758 2 16.208104052026012 10.355177588794398 41.695847923961985 31.740870435217612 3 18.224999999999998 13.575000000000001 31.825 36.375 4 17.375 26.0 25.7 30.925000000000004 5 19.375 32.475 24.575 23.575 6 16.425 39.95 25.0 18.625 7 10.2 30.9 41.949999999999996 16.950000000000003 8 14.299999999999999 26.900000000000002 36.6 22.2 9 17.125 21.175 37.4 24.3 10-14 17.169999999999998 31.15 29.98 21.7 15-19 18.315 30.345 29.335 22.005 20-24 18.815 30.625000000000004 28.04 22.52 25-29 19.66 31.005 29.035 20.3 30-34 16.02 32.68 30.330000000000002 20.97 35-39 17.06 30.759999999999998 29.12 23.06 40-44 18.685 31.8 28.410000000000004 21.105 45-49 19.134999999999998 31.535000000000004 26.215 23.115 50-54 20.5 30.925000000000004 27.57 21.005 55-59 18.35 31.819999999999997 26.700000000000003 23.13 60-64 18.88877775555111 31.28125625125025 28.370674134826967 21.459291858371675 65-69 19.444861215303828 28.687171792948234 28.037009252313077 23.830957739434858 70-74 17.866253188616017 31.310958835592455 29.000150052518382 21.822637923273145 75-79 18.714357178589296 31.910955477738867 28.009004502251127 21.36568284142071 80-84 18.337506259389084 32.06309464196295 27.481221832749124 22.118177265898847 85-89 18.668204382490096 30.837887980745123 29.00767186481472 21.486235771950057 90-94 18.139838377754355 34.78391808462581 26.381569040807108 20.69467449681273 95-99 18.727025529986406 33.29976333148698 27.5290800140994 20.444131124427212 100-104 18.366521783078944 30.951177600323458 29.530981502072173 21.15131911452542 105-109 20.75864520387955 30.56415985375514 27.659574468085108 21.017620474280204 110-114 19.124177001990507 30.66911652120655 28.025315163578828 22.18139131322411 115-119 18.99835289273214 29.44718962322421 31.037677578752316 20.516779905291333 120-124 18.33679833679834 30.254677754677754 29.412681912681915 21.995841995841996 125-129 18.19859726836471 33.50735643094447 24.996044929599748 23.298001371091072 130-134 21.128945960406632 31.690743713215625 26.800428036383096 20.37988228999465 135-139 19.979279131904683 32.03555264736354 24.75598451387753 23.229183706854243 140-144 19.634728544465414 31.86965693349617 24.830687243255245 23.664927278783168 145-149 19.407633933627817 30.903839619435953 24.951863178162874 24.73666326877336 150 18.267581475128644 28.530588907947397 24.842767295597483 28.359062321326473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 2.0 13 2.0 14 1.0 15 0.5 16 0.0 17 1.0 18 2.5 19 3.0 20 3.5 21 3.5 22 4.5 23 4.5 24 2.5 25 2.5 26 3.0 27 30.0 28 36.0 29 22.0 30 42.5 31 77.0 32 99.5 33 113.5 34 112.0 35 103.5 36 178.0 37 227.5 38 227.5 39 257.0 40 248.5 41 254.0 42 310.0 43 281.0 44 210.0 45 194.0 46 176.5 47 189.5 48 150.0 49 77.5 50 65.5 51 71.5 52 52.0 53 16.0 54 14.5 55 14.0 56 5.5 57 5.0 58 13.5 59 29.5 60 24.0 61 7.0 62 5.0 63 5.0 64 3.0 65 4.0 66 5.5 67 2.5 68 0.5 69 0.0 70 0.5 71 1.0 72 1.5 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 60-61 1.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0 74-75 0.0 76-77 0.0 78-79 2.0 80-81 1.0 82-83 4.0 84-85 1.0 86-87 1.0 88-89 4.0 90-91 0.0 92-93 2.0 94-95 8.0 96-97 7.0 98-99 6.0 100-101 4.0 102-103 10.0 104-105 6.0 106-107 7.0 108-109 6.0 110-111 12.0 112-113 8.0 114-115 15.0 116-117 16.0 118-119 10.0 120-121 22.0 122-123 19.0 124-125 23.0 126-127 24.0 128-129 15.0 130-131 28.0 132-133 28.0 134-135 23.0 136-137 31.0 138-139 26.0 140-141 28.0 142-143 15.0 144-145 42.0 146-147 34.0 148-149 12.0 150-151 3498.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.375 #Duplication Level Percentage of deduplicated Percentage of total 1 50.36789297658862 18.825 2 18.996655518394647 14.2 3 10.96989966555184 12.3 4 7.558528428093646 11.3 5 3.2107023411371234 6.0 6 2.608695652173913 5.8500000000000005 7 1.3377926421404682 3.5000000000000004 8 0.9364548494983277 2.8000000000000003 9 0.7357859531772575 2.475 >10 3.2107023411371234 20.200000000000003 >50 0.0 0.0 >100 0.06688963210702341 2.55 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCG 102 2.55 No Hit GCCAAACTCATCGACCGGTGTCACCTTTCAACGATCCTGCACTGACCAAT 48 1.2 No Hit CTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTACAAGGG 46 1.15 No Hit GTCCACATCAACCACCTCTGTCGGGGGCTCTATCCCAAAAGTTGCACCCA 29 0.7250000000000001 No Hit GTCCGCACAAATTGCTTTCTTGGTGACTGGTTTTTTTTGTTCTCCGGGGC 26 0.65 No Hit CCCCAGTTCATCAAACATTGCACAAATGACTGTTTCAACACTATCTACAG 25 0.625 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 25 0.625 No Hit GTGTTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAG 24 0.6 No Hit CTGCCAAACTCATCGACCGGTGTCACCTTTCAACGATCCTGCACTGACCA 24 0.6 No Hit CCCTGATCCGTTTTTTAGCATATAGCTGAAATGCCCGTGCGATGCCACAG 22 0.5499999999999999 No Hit CCCCACTCTTTGATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCGT 20 0.5 No Hit GCCACATTTATCATCATTTGCTCCAGGTATTAAATCCCGATGAGACCCCA 20 0.5 No Hit CTAGCATCCATGCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCTC 19 0.475 No Hit GTTTGACTTCAGCAGATCTTTCAGCTCATCAACAATTGCACGGAAGCAGA 19 0.475 No Hit GCTTTCTTGGTGACTGGTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATT 18 0.44999999999999996 No Hit CATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGTTTTGATTC 18 0.44999999999999996 No Hit GCCTTTTGCAGCACCAAGTGCAATTTTCATCCTGATGGCCCACGGAAGAG 18 0.44999999999999996 No Hit GTTCGGTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGT 17 0.42500000000000004 No Hit CCGGAAGTCTGAGGAATGATGAATACCCAACCCCTGGACAGGTTTAAGAT 17 0.42500000000000004 No Hit GGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTATAT 16 0.4 No Hit GTGCTTTTTTTTCTCTCAACCTGTAAATTTATGGTCGACTAAAAAAATGT 16 0.4 No Hit GTCACATATATCAATTGATTTTGCAAATTGCTTGAGTTCGGTCCATGGAT 16 0.4 No Hit GGAGCATTGTGGAAGCATGGTTCAATGGACATGCCGCGGCCACAAGCCGG 15 0.375 No Hit GCCCATTTTTCTGCAAGCCTCACAAGGATAACAATGTATGGAGTATGCTT 15 0.375 No Hit CTTGCACTTTCCACAGAGAACATTGCGTGATAAAGATAGCTTTTTGGTAG 15 0.375 No Hit CCTGAATCAGATCCCCTCAGGGAGAATGACGCATCACTATGTCTTCTGGA 14 0.35000000000000003 No Hit CCTCACTCCCGCACCACGACAACCATGACAAGTAGCCGTTGCCCCACTCT 14 0.35000000000000003 No Hit TGCCAAACTCATCGACCGGTGTCACCTTTCAACGATCCTGCACTGACCAA 14 0.35000000000000003 No Hit CCCAAACTCTCTCTCGGCGGATAATGCGACTGCAACAAAAAAAAAAGGGG 14 0.35000000000000003 No Hit CCTCAATGGTTTGACTTCAGCAGATCTTTCAGCTCATCAACAATTGCACG 13 0.325 No Hit CCTCACATTACATAAAACTCAAGAGGTGCAAAGCTTCTGTAAAAAAGAAG 13 0.325 No Hit TTTTTTTTTTCAAAAAACTCGCATAGGGTATTCATTCAAGCTGATAAACC 12 0.3 No Hit CTTCAGTATATCAAGGGCATCACCTAACTTGATAGCTCGTCGATCCAACT 12 0.3 No Hit CTTTCTTGGTGACTGGTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATTG 12 0.3 No Hit GTGACTGGTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATTGGACTCAAG 12 0.3 No Hit CAACAATCTAGCACTTTCCTCTCTACACTTACTATCCATACTTTGATCAA 12 0.3 No Hit CACCGAAGGAGAAATCAAAAATAAATTAGCAACACCCCGAAACAAACAAA 12 0.3 No Hit CTCCCCAGTTCATCAAACATTGCACAAATGACTGTTTCAACACTATCTAC 11 0.27499999999999997 No Hit GAAGGAAGTAAACATAAACTGCCAAACTCATCGACCGGTGTCACCTTTCA 11 0.27499999999999997 No Hit CGCACAAATTGCTTTCTTGGTGACTGGTTTTTTTTGTTCTCCGGGGCTTG 11 0.27499999999999997 No Hit CGGAGTAACACAATTATCCACCGATAATTACAGAAATACTAAAAGGGAAG 11 0.27499999999999997 No Hit GTGCAAAGTATCACTGCCGTACTGCGCAGCAAATGACGACACACATTTCT 11 0.27499999999999997 No Hit CTGCACCACAAGACAGTTTTTTACACAAATTTTCATCAGCAATTTAAATT 11 0.27499999999999997 No Hit CCTCTTCCTATTTTTTTCAACAGAATATCTGAACACTTTTATGAGCTCTG 10 0.25 No Hit CCCGCACCACGACAACCATGACAAGTAGCCGTTGCCCCACTCTTTGATCC 10 0.25 No Hit GCTCGACCCAAAAATTATTGAGGAAGGAAGTAAACATAAACTGCCAAACT 10 0.25 No Hit GTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGTTTTGA 10 0.25 No Hit CCTTACTCTTATATGCATCATGACTGAGTGATAAGAAAAATTGCATCGCT 10 0.25 No Hit GCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTATATG 10 0.25 No Hit GCACCACTGACACCATACTTGAACACAAACATAAATATTGGATCCAAAAC 9 0.22499999999999998 No Hit GCCTAAATGAGACTGTGCATTCTTCAGAATATGCCAGTAGGAGAAGCAAT 9 0.22499999999999998 No Hit CCCATCACTTCCTTCAGTATATCAAGGGCATCACCTAACTTGATAGCTCG 9 0.22499999999999998 No Hit CCCCATCACTTCCTTCAGTATATCAAGGGCATCACCTAACTTGATAGCTC 9 0.22499999999999998 No Hit GTCTCATTCAACTCTTCTGACATCTGAAAGGATCCATTTGTAGAAAGACA 9 0.22499999999999998 No Hit CCCACTCTTTGATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCGTG 9 0.22499999999999998 No Hit GCCACAGCTCACAACACATCCACGAACAAGGCTGGAGACTCCCGTTCCTC 9 0.22499999999999998 No Hit GGGAAATTCAGAGATATGAAGCTATGGTGCAGAGGTCGACCTGACCTAAA 9 0.22499999999999998 No Hit GCATCCATGCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTC 9 0.22499999999999998 No Hit CTGGTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATTGGACTCAAGACAG 9 0.22499999999999998 No Hit CTCCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGAC 9 0.22499999999999998 No Hit CTCAAAATCACTTTCACCTACTCCATCAATATGGACCTTTTTTGGTACAC 8 0.2 No Hit GCTTGAGTTCGGTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGG 8 0.2 No Hit CCGCGAAATTCACGGCTATTTAGATAGCTAGCATCCATGCCCTCTAGAAG 8 0.2 No Hit CTTGGCGCCGACGAGCACAGATGCAAAAACAAAACAGCCCGCCTCTCCTG 8 0.2 No Hit GGGGTAGGTGATCATAAGCATCCTCACAGAAAAGTGAACCATTTTTTTTT 8 0.2 No Hit CTTGAGTTCGGTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGT 8 0.2 No Hit ATTGCTGTTATCCCGGAAGTCTGAGGAATGATGAATACCCAACCCCTGGA 8 0.2 No Hit CTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTTC 8 0.2 No Hit CTGCCTTTTTTTTTAGAGGATCTTTCTGCCTTTTTGTTATTCATTAAAAC 8 0.2 No Hit GGTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGTTTTG 8 0.2 No Hit GAGCGTTGCTCGACCCAAAAATTATTGAGGAAGGAAGTAAACATAAACTG 8 0.2 No Hit CTCCTGAATCAGATCCCCTCAGGGAGAATGACGCATCACTATGTCTTCTG 8 0.2 No Hit ATCATTTGCTCCAGGTATTAAATCCCGATGAGACCCCACATCAGATTTTT 8 0.2 No Hit GCCACACACACATCACCAGCATGAAAATAAAGGAAAGGGTGATGGGTACC 8 0.2 No Hit GCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTT 7 0.17500000000000002 No Hit GGGTAAGAAAAAAGAAAGATATAACTAACACATTGGAGGGAACAAGATGT 7 0.17500000000000002 No Hit GGCTAGCTACCTTCTTTGCACCAGCCAGTCTATAGGGACTGACACGTGTT 7 0.17500000000000002 No Hit ACTGGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTA 7 0.17500000000000002 No Hit GGACGGAGTAGGTGATCATAAGCATCCTCACAGAAAAGTGAACCAATTTT 7 0.17500000000000002 No Hit GCCCCACTCTTTGATCCTTTTCCCTTGCACTTTCCACAGAGAACATTGCG 7 0.17500000000000002 No Hit TGGTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATTGGACTCAAGACAGT 7 0.17500000000000002 No Hit CACACATTTCTGCACCACAAGACAGTTTTTTACACAAATTTTCATCAGCA 7 0.17500000000000002 No Hit GTTCATCGTCACTGGGCTGTTCAAAAGTTTCAAATTCCCCATCACTTCCT 7 0.17500000000000002 No Hit TTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGCGA 7 0.17500000000000002 No Hit GCCACCAGACGATCTTTGTATTTTTTGGAATCTTTAAACTTCAGAACATC 7 0.17500000000000002 No Hit CCATTTTTCTGCAAGCCTCACAAGGATAACAATGTATGGAGTATGCTTGT 7 0.17500000000000002 No Hit TTTTTTTTTCATGCAAGTTTACAGAGCATATCTTCCAGCAGCAGTCTGCT 7 0.17500000000000002 No Hit GCCTTTTTTTTTAGAGGATCTTTCTGCCTTTTTGTTATTCATTAAAACCA 7 0.17500000000000002 No Hit GTTCTTTATTAATGTGATGCTGTTCGCCTCTCGCGATCTCAATTCTCAGA 7 0.17500000000000002 No Hit GCTACATACAGTCAATACAAAACAGAAGTCACAGAAAAAGCAAACTTGGT 7 0.17500000000000002 No Hit ATCATGACTGAGTGATAAGAAAAATTGCATCGCTGCGAAGATTTTTTTTT 7 0.17500000000000002 No Hit TTTTTTTTTTTAAAGTTTGAATGAAAATCAAGCATCACAAAGTCCCCCGT 7 0.17500000000000002 No Hit CTCCACTACCAAAACTACTTGACGATCGAATGCGCCACCGTCCACCATCA 7 0.17500000000000002 No Hit GTGACATCTCTTTTTGGCAACTGAAACAATACTCACTTTATCTTTTTCTT 7 0.17500000000000002 No Hit GGTCGGACGGAGTAGGTGATCATAAGCATCCTCACAGAAAAGTGAACCAT 6 0.15 No Hit GTTTTGTTTCCATCATTGTCCTCCAAACCAAGAGTAAAAAAAAAAAAAAA 6 0.15 No Hit CTCCCGCACCACGACAACCATGACAAGTAGCCGTTGCCCCACTCTTTGAT 6 0.15 No Hit GTATGGAGTATGCTTGTGCACAACAGGATCTTTGTCACATATATCAATTG 6 0.15 No Hit GTCACATGGCACTTGATGTGCTGACGCCTCGATCCTTCAGGGCAGAAGAG 6 0.15 No Hit GCCCATTCCCCTTACTCTTATATGCATCATGACTGAGTGATAAGAAAAAT 6 0.15 No Hit CCCAGAACTCAGAGTCGGTATCAGTAGCTTCTTTTTATCCCTCTTTCCAC 6 0.15 No Hit ACCAATTTAGTGTTATCTGACGGTGCTTTTTTTTCTCTCAACCTGTAAAT 6 0.15 No Hit CTTCAGCAGATCTTTCAGCTCATCAACAATTGCACGGAAGCAGAACATGT 6 0.15 No Hit GCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTTCAACAGAAT 6 0.15 No Hit ACTTATTTTGGGACGGAGTGAGTACAACGAATGTCTTTCGACACCGCGGT 6 0.15 No Hit CCCGCCTCTCCTGTCTTCTCACGAGCGAGCAGAAATCAGAGCGGCAAATG 6 0.15 No Hit CCACAAGACAGTTTTTTACACAAATTTTCATCAGCAATTTAAATTTGCAC 6 0.15 No Hit GGACGGAGTAGGTGATCATAAGCATCCTCACAGAAAAGTGAACCATTTTT 6 0.15 No Hit CCTAACTTGATAGCTCGTCGATCCAACTTCTTTTTTTTTTTAAACCATCG 6 0.15 No Hit GTGCTGACGCCTCGATCCTTCAGGGCAGAAGAGTGCCATGAGAGGAAATT 6 0.15 No Hit CCTCAAACATCTAAAGTAATTACACTACCGCGAAATTCACGGCTATTTAG 6 0.15 No Hit CCACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTACAA 6 0.15 No Hit CTGTACCAATTTAGTGTTATCTGACGGTGCTTTTTTTTCTCTCAACCTGT 6 0.15 No Hit ACCACCTCAAACATCTAAAGTAATTACACTACCGCGAAATTCACGGCTAT 6 0.15 No Hit CTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTTCAACAGAATAT 6 0.15 No Hit GCCCGCCTCTCCTGTCTTCTCACGAGCGAGCAGAAATCAGAGCGGCAAAT 6 0.15 No Hit ATCTACAGAACGCAAATGAGAAACCTCAATAGTAACTGATTAAGCTGAGA 6 0.15 No Hit GTGCCATAGTGCCACACACACATCACCAGCATGAAAATAAAGGAAAGGGT 6 0.15 No Hit CTTATTTTGGGACGGAGTGAGTACAACGAATGTCTTTCGACACCGCGGTC 6 0.15 No Hit CGGTGCTTTTTTTTCTCTCAACCTGTAAATTTATGGTCGACTAAAAAAAT 6 0.15 No Hit CTCCAAAGCGGCAATTATTGGTCCACTTAAAGTAAGTTGTGCACTTTAAT 6 0.15 No Hit CCAATTTAGTGTTATCTGACGGTGCTTTTTTTTCTCTCAACCTGTAAATT 6 0.15 No Hit GTCAAATTAACAAGTGATGCAAAAAAAGTGATGCAGTCTGGAGGTGACGG 6 0.15 No Hit GTGTTATCTGACGGTGCTTTTTTTTCTCTCAACCTGTAAATTTATGGTCG 6 0.15 No Hit GCACAAATTGCTTTCTTGGTGACTGGTTTTTTTTGTTCTCCGGGGCTTGT 6 0.15 No Hit GTACCACTGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTA 6 0.15 No Hit TGTTACAAAGGTCACAAGAGTCAGTGAACAGTAATTACTTGTAACACAGC 6 0.15 No Hit CTCGCATAGGGTATTCATTCAAGCTGATAAACCAAAACAAGTGCATTTTG 6 0.15 No Hit CTGGTGAAAAGTTATTTGTGGCAGCTTCCATCTCAGAGAATTCGAACCTT 6 0.15 No Hit CAGAGGTAAAGAGGGATTGCTGTTATCCCGGAAGTCTGAGGAATGATGAA 6 0.15 No Hit GTTGCAATAAATCAACGTGTAGCCTTAATCTCCATAATAGTATGGCTGCA 6 0.15 No Hit CCTCTAGAAGCCTGATACAAACATCTGAACATTCCTCTTCCTATTTTTTT 6 0.15 No Hit GGGAGAATGACGCATCACTATGTCTTCTGGAAGAGAAAGGTGGTGATGAA 6 0.15 No Hit ATCGCCATGAAAAATTCAGACATATGCTTGTCATTTATCTTATTAATCAT 5 0.125 No Hit AGCTCATCAACAATTGCACGGAAGCAGAACATGTACTGGTCCTCGGTTTG 5 0.125 No Hit CTCCAATTATCTGGACAGCGACGTCAAGAAAAAAATGCCATCGCCAAAGT 5 0.125 No Hit CCTACGTCCACGAACCAAAAACTTCACAGAAACCAGGCTTAAACATGTTA 5 0.125 No Hit GGCACTGATACTGCTATAATAGCTAAATGACACTAGATCATACAGAACTA 5 0.125 No Hit TGGAATTTAAACTAAAACTATCTATATGTACCTATTTTATTTACAAGGGC 5 0.125 No Hit GGTTATTTAGCTAGTGTTTATAGATGTCGTGGCTGGTTGAAGCTCCAAAA 5 0.125 No Hit GCCTTATTTCTTTGGTTTAGAGGGCAGGCAGACTGCACCCTGAGACCCCT 5 0.125 No Hit ATCAGCCCATTCCCCTTACTCTTATATGCATCATGACTGAGTGATAAGAA 5 0.125 No Hit ACCTCAAACATCTAAAGTAATTACACTACCGCGAAATTCACGGCTATTTA 5 0.125 No Hit AGCTACATACAGTCAATACAAAACAGAAGTCACAGAAAAAGCAAACTTGG 5 0.125 No Hit AGTTGAGGTAGAAACTGAAGAACACGGCATACTAAATCCCTTATGATTGC 5 0.125 No Hit CGGTCCATGGATTGTGCAGCCGTAAATCATCCAAAAAGTGGTCTGGTTTT 5 0.125 No Hit CTGGCTATACATAGGTTAGTACCACTGGAATTTAAACTAAAACTATCTAT 5 0.125 No Hit GTATGCTTGTGCACAACAGGATCTTTGTCACATATATCAATTGATTTTGC 5 0.125 No Hit CCAAGAATTACTATATTAATATGTAAGTTATATGATACGACACCCTGATC 5 0.125 No Hit GTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATTGGACTCAAGACAGTCT 5 0.125 No Hit GGCAGCTATATTGTGACAATGTCCAAGGGAAAAAAAAGAAGAATGGACAT 5 0.125 No Hit CACCACAAGACAGTTTTTTACACAAATTTTCATCAGCAATTTAAATTTGC 5 0.125 No Hit GCATCACCTAACTTGATAGCTCGTCGATCCAACTTCTTTTTTTTTTTAAA 5 0.125 No Hit CTACCATCTCTATTTACAACAGTGGGCACACAGGTCACCATGGGTTACTA 5 0.125 No Hit CTCCAATCTCATGGGGAGGTACAACAGCTGAAATGAAATTGCGCTCAAAA 5 0.125 No Hit GTACCAATTTAGTGTTATCTGACGGTGCTTTTTTTTCTCTCAACCTGTAA 5 0.125 No Hit GGTGACTGGTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATTGGACTCAA 5 0.125 No Hit CTGGAAGAGAAAGGTGGTGATGAATCTTTTTACAAAATCAGTAGACAAGC 5 0.125 No Hit ATCTGACGGTGCTTTTTTTTCTCTCAACCTGTAAATTTATGGTCGACTAA 5 0.125 No Hit CTCGGTTTGGACCATTCCATTTCGTTGGGACCTAAATTTTTTTACAGTTT 5 0.125 No Hit CACGAAATTTCACAAGGGCTGCTGAGAAATAAAGAACTTCAATGGCACCA 5 0.125 No Hit TTCTTCTTTTTCTTCTTCTCGCCATCTGCATCAGCTAAAGGGGCTTCCTC 5 0.125 No Hit CCTCAATGCCTGCACATTTTTTGGTGTCAGAATAATACTATCACGCTGAT 5 0.125 No Hit CTCCATAATAGTATGGCTGCAATGAAATATTGTGATATAACATGAGCAAT 5 0.125 No Hit GAGTATGCTTGTGCACAACAGGATCTTTGTCACATATATCAATTGATTTT 5 0.125 No Hit GGCTCGTCGATCCAACTTCTTTTTTTTTTTAAACCATCGTTGATCCAACT 5 0.125 No Hit CCGCACCACGACAACCATGACAAGTAGCCGTTGCCCCACTCTTTGATCCT 5 0.125 No Hit CTCACAGAAAAGTGAACCATTTTTTTTTGGCAAACGAACGTGGCCCACAT 5 0.125 No Hit ATCCTGATGGCCCACGGAAGAGGAAACGACCTCCTAAAAAGATGGTTCTC 5 0.125 No Hit GGCAGATCTTTCAGCTCATCAACAATTGCACGGAAGCAGAACATGTACTG 5 0.125 No Hit ACAGAAAAGAAAAACAATTGGAACGAACGTTATAGTCTGAGAAAAAAATA 5 0.125 No Hit ATCCACCGATAATTACAGAAATACTAAAAGGGAAGTAAAAAAGGCAAAAA 5 0.125 No Hit ATCAACAATTGCACGGAAGCAGAACATGTACTGGTCCTCGGTTTGGACCA 5 0.125 No Hit GGTGCTTTTTTTTCTCTCAACCTGTAAATTTATGGTCGACTAAAAAAATG 5 0.125 No Hit GTTGCTACAAGTATTTTTGCGTATGTTCCCTCAATAATTGGTAGTGCTGC 5 0.125 No Hit AGCCCATTCCCCTTACTCTTATATGCATCATGACTGAGTGATAAGAAAAA 5 0.125 No Hit GACTGGTTTTTTTTGTTCTCCGGGGCTTGTTCATCCATTGGACTCAAGAC 5 0.125 No Hit CTGAATCAGATCCCCTCAGGGAGAATGACGCATCACTATGTCTTCTGGAA 5 0.125 No Hit TATCAATTGATTTTGCAAATTGCTTGAGTTCGGTCCATGGATTGTGCAGC 5 0.125 No Hit GCTAGCATCCATGCCCTCTAGAAGCCTGATACAAACATCTGAACATTCCT 5 0.125 No Hit GGAAGGAAGTAAACATAAACTGCCAAACTCATCGACCGGTGTCACCTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGCA 10 0.007596589 139.9375 3 CTAACTG 20 0.0070602535 27.987501 50-54 >>END_MODULE ERR5262811 read2 length is 60-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262811_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 60-150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1785 37.0 37.0 37.0 37.0 37.0 2 35.8905 37.0 37.0 37.0 37.0 37.0 3 36.034 37.0 37.0 37.0 37.0 37.0 4 36.0235 37.0 37.0 37.0 37.0 37.0 5 36.183 37.0 37.0 37.0 37.0 37.0 6 36.14 37.0 37.0 37.0 37.0 37.0 7 35.999 37.0 37.0 37.0 37.0 37.0 8 36.167 37.0 37.0 37.0 37.0 37.0 9 36.073 37.0 37.0 37.0 37.0 37.0 10-14 36.13009999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.1787 37.0 37.0 37.0 37.0 37.0 20-24 36.126599999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.1473 37.0 37.0 37.0 37.0 37.0 30-34 36.0492 37.0 37.0 37.0 37.0 37.0 35-39 36.0181 37.0 37.0 37.0 37.0 37.0 40-44 36.0185 37.0 37.0 37.0 37.0 37.0 45-49 36.018299999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.0041 37.0 37.0 37.0 37.0 37.0 55-59 35.89149999999999 37.0 37.0 37.0 37.0 37.0 60-64 35.869372193048264 37.0 37.0 37.0 37.0 37.0 65-69 35.87781945486371 37.0 37.0 37.0 37.0 37.0 70-74 35.768277261911784 37.0 37.0 37.0 37.0 37.0 75-79 35.77908954477239 37.0 37.0 37.0 37.0 37.0 80-84 35.74740392359968 37.0 37.0 37.0 37.0 37.0 85-89 35.69919071066878 37.0 37.0 37.0 37.0 37.0 90-94 35.637858098927964 37.0 37.0 37.0 37.0 37.0 95-99 35.64015502346408 37.0 37.0 37.0 37.0 37.0 100-104 35.5313756031975 37.0 37.0 37.0 37.0 37.0 105-109 35.43989804749369 37.0 37.0 37.0 37.0 37.0 110-114 35.42547847444873 37.0 37.0 37.0 37.0 37.0 115-119 35.483941354529215 37.0 37.0 37.0 37.0 37.0 120-124 35.36637881614699 37.0 37.0 37.0 34.6 37.0 125-129 35.329746593593185 37.0 37.0 37.0 32.2 37.0 130-134 35.24553604186584 37.0 37.0 37.0 29.8 37.0 135-139 35.25191121039428 37.0 37.0 37.0 29.8 37.0 140-144 35.20490237807248 37.0 37.0 37.0 27.4 37.0 145-149 35.157121322943375 37.0 37.0 37.0 25.0 37.0 150 35.21505989731888 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 1.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 1.0 22 2.0 23 3.0 24 4.0 25 15.0 26 14.0 27 14.0 28 25.0 29 21.0 30 46.0 31 46.0 32 72.0 33 142.0 34 239.0 35 623.0 36 2510.0 37 219.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.9 25.4 9.35 27.35 2 28.125 25.825 30.925000000000004 15.125 3 21.575 28.275 33.300000000000004 16.85 4 25.0 31.95 23.95 19.1 5 25.275 34.5 23.674999999999997 16.55 6 23.05 35.3 23.849999999999998 17.8 7 20.5 22.95 38.824999999999996 17.724999999999998 8 23.125 25.35 27.400000000000002 24.125 9 23.35 22.35 32.074999999999996 22.225 10-14 25.2 27.744999999999997 26.31 20.745 15-19 24.995 26.400000000000002 27.555000000000003 21.05 20-24 25.025 27.055 27.339999999999996 20.580000000000002 25-29 24.98 27.675 27.43 19.915 30-34 24.025 27.85 27.605 20.52 35-39 24.335 28.294999999999998 27.04 20.330000000000002 40-44 23.52 27.22 28.51 20.75 45-49 24.515 26.840000000000003 28.449999999999996 20.195 50-54 23.445 28.035 28.26 20.26 55-59 24.169999999999998 27.41 28.835 19.585 60-64 23.059611922384477 27.18043608721744 28.720744148829763 21.039207841568313 65-69 23.585896474118528 27.53188297074269 28.047011752938232 20.83520880220055 70-74 23.128094833191618 28.10983844345521 29.300255089281247 19.461811634071925 75-79 23.33166583291646 27.22361180590295 28.574287143571787 20.870435217608804 80-84 23.610415623435152 26.720080120180274 30.195292939409114 19.474211316975463 85-89 22.00270771699343 27.36298450584165 29.850072707215563 20.784235069949357 90-94 22.822868041961552 26.647593233950712 29.192390704211213 21.337148019876526 95-99 23.651744800845965 26.00332343018279 29.09008509995468 21.254846669016565 100-104 24.14333367027191 26.402506823006167 29.470332558374608 19.983826948347318 105-109 23.551515767023815 26.603361600568732 30.117300563652062 19.727822068755398 110-114 23.564538355535138 26.963711529627926 29.24003470627265 20.231715408564284 115-119 23.86246654313362 27.100061766522543 29.524397776405188 19.513073913938644 120-124 22.385654885654883 27.92099792099792 29.54261954261954 20.150727650727653 125-129 24.56362389917207 27.137056372936776 28.476506881822495 19.82281284606866 130-134 23.71983519717481 27.615174701696183 28.56225587243833 20.102734228690675 135-139 23.470055634340568 27.59354205301625 30.337078651685395 18.599323660957783 140-144 24.01576989283136 26.458992725859293 29.918374146260202 19.606863235049143 145-149 24.895444783542445 27.348253645303494 28.309031310048603 19.44727026110546 150 21.0781517398745 30.576155162578438 30.576155162578438 17.769537934968625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 2.0 19 2.0 20 0.0 21 1.0 22 1.0 23 0.5 24 3.0 25 6.0 26 5.5 27 4.5 28 14.5 29 21.0 30 22.5 31 35.0 32 39.5 33 52.0 34 78.0 35 90.5 36 109.5 37 130.0 38 155.0 39 176.5 40 195.0 41 204.0 42 210.5 43 247.5 44 253.5 45 237.0 46 240.5 47 241.5 48 199.0 49 146.5 50 117.5 51 89.5 52 83.5 53 84.0 54 68.0 55 54.5 56 61.5 57 57.5 58 35.0 59 21.5 60 23.5 61 23.5 62 21.0 63 21.0 64 14.0 65 15.5 66 14.5 67 12.5 68 9.5 69 6.0 70 9.0 71 7.5 72 6.5 73 5.5 74 3.0 75 2.0 76 1.5 77 1.5 78 1.0 79 0.5 80 2.5 81 3.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 60-61 1.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0 74-75 0.0 76-77 0.0 78-79 2.0 80-81 1.0 82-83 4.0 84-85 1.0 86-87 1.0 88-89 4.0 90-91 0.0 92-93 2.0 94-95 8.0 96-97 7.0 98-99 6.0 100-101 4.0 102-103 10.0 104-105 6.0 106-107 7.0 108-109 6.0 110-111 12.0 112-113 8.0 114-115 15.0 116-117 16.0 118-119 10.0 120-121 22.0 122-123 19.0 124-125 23.0 126-127 24.0 128-129 15.0 130-131 28.0 132-133 29.0 134-135 23.0 136-137 31.0 138-139 26.0 140-141 28.0 142-143 15.0 144-145 33.0 146-147 34.0 148-149 12.0 150-151 3506.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.2 #Duplication Level Percentage of deduplicated Percentage of total 1 56.17816091954023 29.325000000000003 2 22.126436781609197 23.1 3 11.015325670498084 17.25 4 4.885057471264368 10.2 5 1.9157088122605364 5.0 6 1.6762452107279693 5.25 7 1.1494252873563218 4.2 8 0.4789272030651341 2.0 9 0.14367816091954022 0.675 >10 0.43103448275862066 3.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATCTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAG 16 0.4 No Hit GTTCGCTCTTCGCGGAGACCACCGGCGAACCGTAGTCCCCAGCCATAGCC 16 0.4 No Hit AGAAGATGCGCTTGCTGGAGTTCAAGCTGCAAAAGCTGCAGAAATAAGAT 15 0.375 No Hit GGGGACGCAGACAGAAGCGTGGTGAAGATGTGGTGCACACCATGAAGGTT 15 0.375 No Hit CAGACAGAAGCGTGGTGAAGATGTGGTGCACACCATGAAGGTTTCGTTAG 13 0.325 No Hit CTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAG 13 0.325 No Hit CGGCAGGACTTGCAGGCCAGGAACCTACAGATTGGTGAGATCATATCCCA 11 0.27499999999999997 No Hit ATTTGAACAGACATGCAACTCCGGTCCGTAAACTTGAGAATCCCACATGT 11 0.27499999999999997 No Hit GATAGATACAAATCCTTCCTTCTTTTCCCAGTTCACTGTTGTCATTGCTA 10 0.25 No Hit GTTTTGGTTGCGTCTTCAAAGGTTGGATTGAAGAGAATGGAACTGCTCCT 9 0.22499999999999998 No Hit GGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGA 9 0.22499999999999998 No Hit GAAGGTGTTGCGCAAGAGATTTGCTCGCCAAGATGAAGAATACGATTCCA 9 0.22499999999999998 No Hit AGTTGCAAGGCAAATGATTCCACTCATCCCTCTACTTCAGTCTGTTTTTG 8 0.2 No Hit CGACTGTCTCCAGCCTGTGATTAACATTATACCATTACAGTTGCTTGCAT 8 0.2 No Hit CTTACACATGCGCTTCTGCAAGGAGAGAATGAGAATCAAATGAGAGAGAA 8 0.2 No Hit AGACAGAAGCGTGGTGAAGATGTGGTGCACACCATGAAGGTTTCGTTAGA 8 0.2 No Hit TCCAGAGAGAAAGGTTGGGGAGTGGGGTGGAGAGGTGATTTTGACCTCCT 8 0.2 No Hit CATTACAGTTGCTTGCATACCATCTGACTGTTCTTCGTGGATTCAACGTC 8 0.2 No Hit AGAGATTTGCTCGCCAAGATGAAGAATACGATTCCATGCTTGGTGTCACT 8 0.2 No Hit GCAATAACAGCTGACAAGGAGGCTTTGAGAGCTTGGTGATGGAAGAAAAT 8 0.2 No Hit CCGACCATGGAGTACCAACCAGTACTGATGCTGTAAGACAAATCCGAAAA 8 0.2 No Hit GTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAG 8 0.2 No Hit GTCGTGGGTTTTCATAGCTCGGATATGTGTGACTGATCTTACACATGCGC 7 0.17500000000000002 No Hit GGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATT 7 0.17500000000000002 No Hit TATGAAGAGGTAGATTTGATCTCCAAGGGTGGAAACTATGGGTGGCGGGC 7 0.17500000000000002 No Hit CATGGAGTACCAACCAGTACTGATGCTGTAAGACAAATCCGAAAACGGTT 7 0.17500000000000002 No Hit GCAGACTGATCGAAGTCCCAGAGGTTGCCGACTGTCTCCAGCCTGTGATT 7 0.17500000000000002 No Hit GTCCTATGGTTTTGGCATTACAACAATGCATAAATTTTTCGATTATGTTG 7 0.17500000000000002 No Hit GACAATTATTAACAACATGCTTGAGTATCATCCTGTAATTTCCTTCACCT 7 0.17500000000000002 No Hit GAGAAGAGTACACATTTGGGAGCTCAGAAGAGGACACCAATGGATGATGT 7 0.17500000000000002 No Hit AGGGGACGCAGACAGAAGCGTGGTGAAGATGTGGTGCACACCATGAAGGT 7 0.17500000000000002 No Hit GTTTATTCGTTTCAGTGTTGACCTGTGCACATCTTTAGTTTTATAGGACT 7 0.17500000000000002 No Hit AAAAGATTGAGTTTGAGAAGGTGTTGCGCAAGAGATTTGCTCGCCAAGAT 7 0.17500000000000002 No Hit ATTAGCTTGGGTGGAAGGAAGAAGAAGAAGAAAATGAGCCCCAAAAAAGT 7 0.17500000000000002 No Hit AGACATCTTTGTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAG 7 0.17500000000000002 No Hit TTACGATTGCAAGGTTTAGGTTTTGATAATTGCTACTGATGCATTGTGCC 7 0.17500000000000002 No Hit AGTTCATCCATCCACTAGTAGCTTCCCTGTGCTTCTTGCAAGCTCTTCTA 7 0.17500000000000002 No Hit CCTGAATTCAAGAGGAAGCTAGACAGGATGGTAGATATCAACACGAAGAT 7 0.17500000000000002 No Hit CCAACAAAGACGTGGCCTCGGAGTCGGAGTCGGAGCAGAGGCCACACCCG 7 0.17500000000000002 No Hit GTCCCAGAGGTTGCCGACTGTCTCCAGCCTGTGATTAACATTATACCATT 7 0.17500000000000002 No Hit GGCCCATCCGGAGCTGACACCCCCCAGCCCTGCCCAATAAGCCTTTCGCT 7 0.17500000000000002 No Hit ATTCAAAACAACTTGGAGCTACCATGATTGGATGTTACTCTAGGTCCTGC 7 0.17500000000000002 No Hit CAGATATCGTGTTGGTTGCTGCACGTTCATATGGTCTAACTGGTCTTGTC 7 0.17500000000000002 No Hit CGCGTATGCGTCTGGCCTCTTTCATGGACGAGGGGAGTGAGGTTGAATTA 7 0.17500000000000002 No Hit AGAAGATTCTCTTGGGATGTCTCCCTCAAGCCGGAGATGGAGACTTTGGC 7 0.17500000000000002 No Hit ATGAAGAATACGATTCCATGCTTGGTGTCACTCGAGAGGCCCATCCGGAG 7 0.17500000000000002 No Hit GAGAGAATGAGAATCAAATGAGAGAGAAAACTGACAATGCCTTGGTTCAT 6 0.15 No Hit TTAAATTTCTGATTTCAGCGGCCAGTTTATTCGTTTCAGTGTTGACCTGT 6 0.15 No Hit TGAGAATCAAATGAGAGAGAAAACTGACAATGCCTTGGTTCATTTCTATG 6 0.15 No Hit AGATTGTGCACACATCCTCTACAGTACATGGCCAGATATACATACCAGAG 6 0.15 No Hit GTTGATTTTGCTGAATTTGAGCCCAAGCTTGTCTACTGATTTTGTAAAAA 6 0.15 No Hit CAGAATGAAAAGCTGCTGCCAGAAATCGGTGCAACTGCTGAAGCAGCAGC 6 0.15 No Hit GGATCTTCTTCCTCCTCGGCCGAGCGCCGCCACCCATCCCGCTGCCGAGC 6 0.15 No Hit ACTACACCTAGATTTGATGTTGGTAGTAGTTTTTCCATTCTACTTAGTAG 6 0.15 No Hit AAACATTATAAGAGCTATGGCAATCTTCGTAGAGTTCAAATGTTGTGGTT 6 0.15 No Hit GTCTATCCTTCACGTATGATGGGGTTCCTCTTCTAGCTATGTTGGATGAG 6 0.15 No Hit GTTCTTTGTTGAAATTAGATGGTATCTGCAGGGCAGCAGATATCGTGTTG 6 0.15 No Hit TAGCACTTTTGATTTTGGGCGAGAGGGCCCTGTTTCTGGAAAAAAATCTG 6 0.15 No Hit GAAGATTCTCTTGGGATGTCTCCCTCAAGCCGGAGATGGAGACTTTGGCC 6 0.15 No Hit CTGAGATCATCGCCGCGTACCTCATGCCCCCGATCGAGTCTGGATCTGTT 6 0.15 No Hit TTTTGACTTCAAAGAAACTTCCAGAATGATGCATCTGCTCATTTTTGAAA 6 0.15 No Hit CGGATATATTCTTGATGTCACTCCAGGAAAGATTGAAAAAATGCTCTTTG 6 0.15 No Hit AGATGGTATCTGCAGGGCAGCAGATATCGTGTTGGTTGCTGCACGTTCAT 6 0.15 No Hit GTGACCATGTACCTGAATGACTGCCAACGTACTGCATTCTACGAAGGAAT 6 0.15 No Hit AGATAGTCGAAAAACTTCTTATTGCTGCAGTTGCTGATGCTGACGTTGGT 6 0.15 No Hit ATAACTGTTATGCTTGTCACAACTTGCTTGATGTCACTGGTTATAGTTCT 6 0.15 No Hit GTGGAGTAATGACTGAGTGTGGCACCAACCCTCCTGTTGAACACATTCTT 6 0.15 No Hit AACATCCTATAGTTGTACACTGCAGTGCAGGCATTGGAAGAACTGGTGCT 6 0.15 No Hit GCAGCATCAGGCAGGCGATGAGAACAGAAGACAGGAGGATCAGGCAGTAG 6 0.15 No Hit GCAAGCAGGAAGCATTATTTATAGAACCGATTACTGAATAGAGGAAAAGA 6 0.15 No Hit CTTGGTGTCACTCGAGAGGCCCATCCGGAGCTGACACCCCCCAGCCCTGC 6 0.15 No Hit GTAAAGTTTCTCATATTATTCTTGGGAAGGCTGTATTTGGTCCAGACTTC 6 0.15 No Hit TCTGAAACCGGACTCTGGAATGCTGAATCCTGCATTACAGTACTTGGTAC 6 0.15 No Hit GGCGTTCGCGAGCAAGAACCCGGCCTTGCTGAAGAACATGACTCTCCCGT 6 0.15 No Hit GTTCATCCATCCACTAGTAGCTTCCCTGTGCTTCTTGCAAGCTCTTCTAA 6 0.15 No Hit GAAGTGATGGGGAATTTGAAATTTTTGAACAGCCCAGTGACGATGAAGAG 6 0.15 No Hit CAGGATTAACTGTTGCTGTCAAGACACTCAACCATGATGGACTTCAGGGG 6 0.15 No Hit GTCCAGACTTCAGTGTAGAACCTATTGATGCTATTCCAGTTGAACTCCAG 6 0.15 No Hit GTACACAGGGAGTTGCAAGGCAAATGATTCCACTCATCCCTCTACTTCAG 6 0.15 No Hit GTGATCATTTAGCTATAAATTTTAGTTTAATATTTGTTGTATTGTTTATA 6 0.15 No Hit TTTAGGGCTAAATGTTGTGAAAGCTAGTGTTTGTCTTGATTCTTCTGGCA 6 0.15 No Hit CTTGAATCCACAACACACGACCACCGTGGTCATGATCCTTCCTTGCAACA 5 0.125 No Hit CTTTTTTGATCTCTCTCGATTGTTTATTTCAGTCTTCGGCATAAGCCTGT 5 0.125 No Hit GTTAACTACCGAAGTTCCATAGTTTCACTCCCAAGTAATTATTCTCAATA 5 0.125 No Hit GAAAGCTTTGCAGCTTCCAGTATGATATAACCCCCCAGGACGGAAGTATT 5 0.125 No Hit CTTTGTTGAAATTAGATGGTATCTGCAGGGCAGCAGATATCGTGTTGGTT 5 0.125 No Hit CCCATCCGGAGCTGACACCCCCCAGCCCTGCCCAATAAGCCTTTCGCTTA 5 0.125 No Hit ACTCTGAGTGCAACGTCATCATAACACTGGAGGCGCAGCAGAAGGCGCCA 5 0.125 No Hit GGTCCCATCCCATCAAAGAAAGTGGAATGATTCAACAGCCCGGGACAACA 5 0.125 No Hit CTTGTGGGAACTTTTTGCTAGAAGATGAAATATTGAATGTTTGATATCTG 5 0.125 No Hit AGAAAACTGACAATGCCTTGGTTCATTTCTATGTCTCATCTATCTATTTA 5 0.125 No Hit TATTATTCATGAATCGAGAAATATAGACCATACTGTGACTGTGAAGAACA 5 0.125 No Hit GTTCGAGGTCGGTCTGAGGATTAGCTTGGGTGGAAGGAAGAAGAAGAAGA 5 0.125 No Hit GCACATGTGCACATGCTCGAAATGTGCGAATGAGTTGGTTCGAAGCGGAG 5 0.125 No Hit GCGTATCCCACGTCGTGGTGAGGTGGGTCTGTCTGCAGATGAGATTCAGA 5 0.125 No Hit CAGAGATACAAAGCACTTGACAAATGCACAAGGTCTGGCGATCATAACTG 5 0.125 No Hit CACCTGGTTCGAGAGAAGATTCTCTTGGGATGTCTCCCTCAAGCCGGAGA 5 0.125 No Hit AGAACCTGAAGAGGGTTCCTCTTATTGCCCAACTGGTGTCTGAGATCATC 5 0.125 No Hit AGAGATAGTGTCACTCTTTATCTGGAAGACGAGGATAGCAGTACCCGAAA 5 0.125 No Hit ATGGGGTGGAAACCCTGCCAAATTTCTGAGGAAGCTCACTGATGAGGAGA 5 0.125 No Hit CTGTCGAGAACGCTAAGCCTTTGGAAAAGATTGAGTTTGAGAAGGTGTTG 5 0.125 No Hit GTTCAAGGGGACGCAGACAGAAGCGTGGTGAAGATGTGGTGCACACCATG 5 0.125 No Hit CATAGTTTGGGTCTCTATGGCTTATTCATGCTTGAATTTGAAGAGCATAG 5 0.125 No Hit ATTCCATGCTTGGTGTCACTCGAGAGGCCCATCCGGAGCTGACACCCCCC 5 0.125 No Hit CAATTTAGCAGTTACAGCTGCGTTTGTGGATTTTAATTCAATCACATAGC 5 0.125 No Hit GTTGTACACTGCAGTGCAGGCATTGGAAGAACTGGTGCTTACATCACCAT 5 0.125 No Hit GTTACAGTAGCCATGACACGCACAACCCCAATGGCATGCCGGACAACGGC 5 0.125 No Hit GCTCAATGATGCTGTGAAAGTCAAGTATGTTGAGGAGTCTCCAGGGACAA 5 0.125 No Hit CAATGAAAAAGCTAATAGATGAAGAATTCTCAAAAGATGTCAATGCTCGC 5 0.125 No Hit AGAGTTCTTTGTTGAAATTAGATGGTATCTGCAGGGCAGCAGATATCGTG 5 0.125 No Hit AGAACCTATTGATGCTATTCCAGTTGAACTCCAGGAAACACCTGGTTCGA 5 0.125 No Hit GGAGGCTTTGAGAGCTTGGTGATGGAAGAAAATGGAGTGTGACTGTGTTG 5 0.125 No Hit GTTTGTGCCGCAGGTTGGTTCTGCTGTTCCTAATAGTTTGGAAAAAAATC 5 0.125 No Hit ATTGGTAGTGATGGAAACATTATAAGAGCTATGGCAATCTTCGTAGAGTT 5 0.125 No Hit CATGCTGAAGGGTGCAGGAGTGGCACACGGGACTGCTAATATCCACGATC 5 0.125 No Hit CGGAAAAGTATTCCATCGGTTTCGACATCATTACTACTTGCTGGGGTGCT 5 0.125 No Hit CAAATCCGAAAACGGTTACATAATGTTCGGGAAGAACATCCTATAGTTGT 5 0.125 No Hit GAAGGTCGTTGGATGGGCTAGAAGTCACTACTTGTCATCAGCTATTCATC 5 0.125 No Hit CCGCTGCCGAGCGCCCATCGTCTTCCTCCTCGCGCGAGCGCCTCCACCGC 5 0.125 No Hit CCGCAGGTTGGTTCTGCTGTTCCTAATAGTTTGGAAAAAAATCTAGATGT 5 0.125 No Hit GGAGAGTTCTTTGTTGAAATTAGATGGTATCTGCAGGGCAGCAGATATCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCAGA 10 0.007596589 139.9375 5 GACGCAG 10 0.007596589 139.9375 4 CGCAGAC 10 0.007596589 139.9375 6 GCAGACA 10 0.007596589 139.9375 7 GGGACGC 10 0.007596589 139.9375 2 CAGACAG 10 0.007596589 139.9375 8 GGGGACG 10 0.007596589 139.9375 1 AGACAGA 10 0.007596589 139.9375 9 >>END_MODULE Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947229 spots for ERR5262811.sra Written 1947229 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra Read 1947225 spots for ERR5262811.sra Written 1947225 spots for ERR5262811.sra SRR ids: ['ERR5262811.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_eapjw1ow ERR5262811.sra spots: 38944504 blocks: [[1, 1947225], [1947226, 3894450], [3894451, 5841675], [5841676, 7788900], [7788901, 9736125], [9736126, 11683350], [11683351, 13630575], [13630576, 15577800], [15577801, 17525025], [17525026, 19472250], [19472251, 21419475], [21419476, 23366700], [23366701, 25313925], [25313926, 27261150], [27261151, 29208375], [29208376, 31155600], [31155601, 33102825], [33102826, 35050050], [35050051, 36997275], [36997276, 38944504]] ERR5262811 file size 12851879 ERR5262811 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262811 ERR5262811_1.fastq ERR5262811_2.fastq Input file: ERR5262811_1.fastq Paired file: ERR5262811_2.fastq trimmed: ERR5262811-trimmed-pair1.fastq, ERR5262811-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:26:15 2024 >> started Fri Dec 6 12:27:13 2024 >> done (57.404s) 38944504 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 38944504 (100.00%) read pairs available; of these: 9265 ( 0.02%) trimmed read pairs available after processing 38935239 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 23 1 0.00% 24 0 0.00% 25 0 0.00% 26 1 0.00% 27 1 0.00% 28 1 0.00% 29 1 0.00% 30 1 0.00% 31 1 0.00% 32 3 0.00% 33 0 0.00% 34 1 0.00% 35 4 0.00% 36 5 0.00% 37 6 0.00% 38 3 0.00% 39 3 0.00% 40 0 0.00% 41 0 0.00% 42 1 0.00% 43 0 0.00% 44 1 0.00% 45 2 0.00% 46 1 0.00% 47 4 0.00% 48 2 0.00% 49 269 0.00% 50 290 0.00% 51 317 0.00% 52 353 0.00% 53 317 0.00% 54 335 0.00% 55 404 0.00% 56 413 0.00% 57 505 0.00% 58 574 0.00% 59 664 0.00% 60 756 0.00% 61 874 0.00% 62 1064 0.00% 63 1024 0.00% 64 1109 0.00% 65 1254 0.00% 66 1389 0.00% 67 1545 0.00% 68 1735 0.00% 69 1991 0.01% 70 2273 0.01% 71 2699 0.01% 72 3153 0.01% 73 3439 0.01% 74 3870 0.01% 75 4182 0.01% 76 4528 0.01% 77 5226 0.01% 78 5705 0.01% 79 6351 0.02% 80 7162 0.02% 81 8378 0.02% 82 9251 0.02% 83 10451 0.03% 84 11617 0.03% 85 12985 0.03% 86 13976 0.04% 87 14930 0.04% 88 16683 0.04% 89 17468 0.04% 90 19338 0.05% 91 21165 0.05% 92 23298 0.06% 93 25093 0.06% 94 27617 0.07% 95 29339 0.08% 96 31708 0.08% 97 32717 0.08% 98 34390 0.09% 99 37039 0.10% 100 38683 0.10% 101 40671 0.10% 102 43372 0.11% 103 46719 0.12% 104 48639 0.12% 105 51366 0.13% 106 53611 0.14% 107 55421 0.14% 108 56694 0.15% 109 59696 0.15% 110 62015 0.16% 111 63640 0.16% 112 67097 0.17% 113 68813 0.18% 114 72906 0.19% 115 75325 0.19% 116 78083 0.20% 117 79843 0.21% 118 82751 0.21% 119 83521 0.21% 120 86089 0.22% 121 87856 0.23% 122 89802 0.23% 123 92468 0.24% 124 97006 0.25% 125 98531 0.25% 126 101237 0.26% 127 103078 0.26% 128 103912 0.27% 129 106754 0.27% 130 108300 0.28% 131 108931 0.28% 132 112313 0.29% 133 113902 0.29% 134 116169 0.30% 135 120836 0.31% 136 122186 0.31% 137 124355 0.32% 138 125186 0.32% 139 127593 0.33% 140 129117 0.33% 141 130785 0.34% 142 133722 0.34% 143 136235 0.35% 144 139178 0.36% 145 140465 0.36% 146 145757 0.37% 147 295085 0.76% 148 138032 0.35% 149 138153 0.35% 150 33579349 86.22% 38944504 reads passed initial QC criterion=sequence-density sequence-density=0.79 sequence-density-rank=1 fanout-score=3.39 fanout-score-rank=26 prefix-density=0.96 prefix-fanout=2.8 sequence=TCTCCAGCTCCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=61.74 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=3.3 sequence=TGGCACTTGGCCATGGCGTCGTCGCAGACCATGACCCCCGAGAAGGTCCTGCAGTTGTCGCAGCACTTCGGCCAATACCCCACGGCATTCACCTCTGCAAGGATTCCCATGACCAGGATGGCCTGGAGAACTAGGATCGCCGTGAGCGTGCTGCTCTTCATCGTTGCTTCTTAGATTTCCGCGCAAAGCTGCCGCGCCTTGTCCGGTCTACTTGGGGAATGCTTTTAAGTTG criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=2.33 fanout-score-rank=36 prefix-density=0.47 prefix-fanout=2.2 sequence=GACGCCATTGATG criterion=fanout-score sequence-density=0.13 sequence-density-rank=20 fanout-score=114.39 fanout-score-rank=1 prefix-density=0.82 prefix-fanout=17.8 sequence=CGCCGCCGCCGGAGC ERR5262811 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:27:52 Started mapping on | Dec 06 12:28:17 Finished on | Dec 06 12:30:45 Mapping speed, Million of reads per hour | 947.30 Number of input reads | 38944504 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 37988079 Uniquely mapped reads % | 97.54% Average mapped length | 292.60 Number of splices: Total | 36412877 Number of splices: Annotated (sjdb) | 33709088 Number of splices: GT/AG | 35890329 Number of splices: GC/AG | 440101 Number of splices: AT/AC | 22016 Number of splices: Non-canonical | 60431 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.25 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 394040 % of reads mapped to multiple loci | 1.01% Number of reads mapped to too many loci | 746 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.43% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 562385 562385 562385 N_multimapping 394040 394040 394040 N_noFeature 1615823 36971600 1933500 N_ambiguous 834554 5339 135603 UnstrandedReadsAssigned:35537702 PositiveStrandReadsAssigned:1011140 NegativeStrandReadsAssigned:35918976 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262811 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262811-trimmed-pair1.fastq ERR5262811-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 38,944,504 reads, 36,181,276 reads pseudoaligned [quant] estimated average fragment length: 280.915 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,221 rounds 52973 ERR5262811.ke.tsv 35125 ERR5262811.se.tsv 88098 total ==> ERR5262811.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 657.768 0 0 PNS24247 1044 764.085 234.494 12.3684 PNS24249 1928 1648.08 419.437 10.2568 PNS24246 1044 764.085 234.494 12.3684 PNS24248 1044 764.085 234.494 12.3684 PNS24244 1471 1191.08 267.081 9.03702 PNS24243 293 97.9689 0 0 KQK14069 1603 1323.08 50235.6 1530.2 KQK14071 474 230.84 262.829 45.8867 ==> ERR5262811.se.tsv <== BRADI_1g14170v3 51347 BRADI_1g53295v3 391 BRADI_1g59795v3 976 BRADI_1g07683v3 0 BRADI_1g00485v3 11 BRADI_1g20270v3 951 BRADI_1g74790v3 2773 BRADI_1g09890v3 0 BRADI_1g77505v3 370 BRADI_1g48960v3 0 ERR5262811 completed mapping pipeline successfully