Starting /dee2/code/volunteer_pipeline.sh ERR5262812 current disk space = 1551017947136 free memory = 1598989424 ERR5262812 SRAfilesize e4f855d371b6197b048930da401539c4 ERR5262812.sra ERR5262812.sra file validated ERR5262812 is paired end ERR5262812 is conventional basespace ERR5262812 read1 length is 60-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262812_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 60-150 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.661 37.0 37.0 37.0 37.0 37.0 2 36.5045 37.0 37.0 37.0 37.0 37.0 3 36.626 37.0 37.0 37.0 37.0 37.0 4 36.646 37.0 37.0 37.0 37.0 37.0 5 36.6945 37.0 37.0 37.0 37.0 37.0 6 36.7425 37.0 37.0 37.0 37.0 37.0 7 36.701 37.0 37.0 37.0 37.0 37.0 8 36.7165 37.0 37.0 37.0 37.0 37.0 9 36.729 37.0 37.0 37.0 37.0 37.0 10-14 36.680499999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.6472 37.0 37.0 37.0 37.0 37.0 20-24 36.6173 37.0 37.0 37.0 37.0 37.0 25-29 36.621500000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.5973 37.0 37.0 37.0 37.0 37.0 35-39 36.5377 37.0 37.0 37.0 37.0 37.0 40-44 36.538 37.0 37.0 37.0 37.0 37.0 45-49 36.50789999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.500600000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.434000000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.429686371592894 37.0 37.0 37.0 37.0 37.0 65-69 36.37954488622156 37.0 37.0 37.0 37.0 37.0 70-74 36.31112778194549 37.0 37.0 37.0 37.0 37.0 75-79 36.3327663831916 37.0 37.0 37.0 37.0 37.0 80-84 36.22121924514075 37.0 37.0 37.0 37.0 37.0 85-89 36.32885253971655 37.0 37.0 37.0 37.0 37.0 90-94 36.27629749582636 37.0 37.0 37.0 37.0 37.0 95-99 36.25262838881888 37.0 37.0 37.0 37.0 37.0 100-104 36.19880754870099 37.0 37.0 37.0 37.0 37.0 105-109 36.201135085141416 37.0 37.0 37.0 37.0 37.0 110-114 36.13102670456162 37.0 37.0 37.0 37.0 37.0 115-119 36.15972869860227 37.0 37.0 37.0 37.0 37.0 120-124 36.15003693186192 37.0 37.0 37.0 37.0 37.0 125-129 36.12522458519301 37.0 37.0 37.0 37.0 37.0 130-134 36.04272028916357 37.0 37.0 37.0 37.0 37.0 135-139 36.03061269065047 37.0 37.0 37.0 37.0 37.0 140-144 35.95219901041824 37.0 37.0 37.0 37.0 37.0 145-149 35.9511493602099 37.0 37.0 37.0 37.0 37.0 150 35.94100029949087 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 0.0 24 2.0 25 0.0 26 5.0 27 3.0 28 2.0 29 6.0 30 14.0 31 27.0 32 40.0 33 57.0 34 104.0 35 311.0 36 3105.0 37 322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75 12.425 8.35 37.475 2 18.86886886886887 10.71071071071071 36.211211211211214 34.209209209209206 3 14.124999999999998 15.975 35.275 34.625 4 20.549999999999997 20.05 25.275 34.125 5 26.375 27.025 26.174999999999997 20.424999999999997 6 25.35 30.825000000000003 24.8 19.025 7 17.150000000000002 23.575 42.0 17.275 8 16.05 26.575 27.6 29.775000000000002 9 15.2 22.6 39.074999999999996 23.125 10-14 19.325 26.645000000000003 27.395000000000003 26.634999999999998 15-19 18.68 27.644999999999996 29.044999999999998 24.63 20-24 18.535 28.465 26.669999999999998 26.33 25-29 19.97 30.380000000000003 24.83 24.82 30-34 18.990000000000002 29.065 26.82 25.124999999999996 35-39 18.56 31.009999999999998 24.595 25.835 40-44 19.41 30.28 24.82 25.490000000000002 45-49 19.36 31.259999999999998 25.424999999999997 23.955000000000002 50-54 22.915 28.849999999999998 28.694999999999997 19.54 55-59 18.165 30.564999999999998 31.380000000000003 19.89 60-64 18.78375675135027 31.176235247049412 28.670734146829368 21.369273854770952 65-69 19.059764941235308 31.707926981745437 27.56689172293073 21.66541635408852 70-74 23.055763940985248 30.38259564891223 25.9964991247812 20.56514128532133 75-79 19.029514757378692 31.535767883941972 27.283641820910454 22.151075537768886 80-84 18.854140605454088 33.82536902677008 27.180385288966725 20.140105078809107 85-89 24.20656806217097 28.799197793933317 27.65104036099273 19.343193782902983 90-94 18.48836624956028 31.061862405145984 31.489019548721043 18.96075179657269 95-99 18.709644874899112 34.100080710250204 27.138821630347053 20.05145278450363 100-104 19.505858380030567 31.569026999490575 27.554763117677023 21.370351502801835 105-109 21.69636419816665 29.266659800185398 29.256360078277886 19.780615923370068 110-114 21.49028643242394 33.87966696339739 25.778918154683982 18.851128449494688 115-119 17.745969243867396 32.320544883733305 26.243814186133136 23.689671686266163 120-124 20.07208952012051 29.648160103292447 29.927910479879493 20.351839896707553 125-129 18.415312465917765 35.54367979059876 25.635292834551205 20.40571490893227 130-134 20.235320235320238 36.646686646686646 24.453324453324456 18.664668664668664 135-139 19.76796830786644 32.88058856819468 28.330503678551217 19.020939445387665 140-144 21.08294930875576 31.62442396313364 26.394009216589865 20.898617511520737 145-149 21.96933962264151 28.20754716981132 27.441037735849054 22.382075471698112 150 16.951182988918838 28.930817610062892 27.19377058999701 26.924228811021262 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.5 27 4.5 28 5.0 29 6.5 30 16.0 31 28.5 32 55.0 33 60.0 34 70.5 35 137.0 36 152.0 37 261.5 38 377.5 39 261.0 40 190.5 41 251.5 42 296.5 43 266.5 44 203.5 45 166.5 46 172.5 47 152.5 48 139.5 49 121.5 50 87.5 51 78.5 52 52.0 53 35.0 54 21.0 55 23.5 56 29.5 57 21.0 58 13.5 59 12.0 60 10.0 61 12.5 62 25.0 63 19.5 64 6.5 65 9.5 66 12.5 67 11.0 68 12.0 69 18.0 70 24.5 71 22.0 72 17.0 73 19.0 74 11.0 75 3.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 60-61 1.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 1.0 84-85 5.0 86-87 5.0 88-89 4.0 90-91 0.0 92-93 10.0 94-95 3.0 96-97 7.0 98-99 21.0 100-101 16.0 102-103 13.0 104-105 16.0 106-107 21.0 108-109 26.0 110-111 31.0 112-113 25.0 114-115 28.0 116-117 15.0 118-119 16.0 120-121 17.0 122-123 19.0 124-125 15.0 126-127 26.0 128-129 32.0 130-131 25.0 132-133 15.0 134-135 32.0 136-137 35.0 138-139 23.0 140-141 22.0 142-143 27.0 144-145 38.0 146-147 32.0 148-149 37.0 150-151 3339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.8 #Duplication Level Percentage of deduplicated Percentage of total 1 42.23790322580645 10.475 2 17.641129032258064 8.75 3 12.298387096774194 9.15 4 7.459677419354839 7.3999999999999995 5 3.931451612903226 4.875 6 2.82258064516129 4.2 7 2.4193548387096775 4.2 8 2.1169354838709675 4.2 9 1.411290322580645 3.15 >10 7.358870967741936 35.075 >50 0.20161290322580644 3.65 >100 0.10080645161290322 4.875 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACGTA 195 4.875 No Hit CTCCTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACG 81 2.025 No Hit GTTCGAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATAT 65 1.625 No Hit GCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTACTGGGTCTCAA 49 1.225 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 44 1.0999999999999999 No Hit CTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACGTAG 44 1.0999999999999999 No Hit ATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTAT 42 1.05 No Hit GTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACGTAGTATT 40 1.0 No Hit AGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 39 0.975 No Hit CACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCG 36 0.8999999999999999 No Hit GTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAACATGCACG 35 0.8750000000000001 No Hit GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 32 0.8 No Hit CCAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGC 31 0.775 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 31 0.775 No Hit GCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATA 30 0.75 No Hit CCTGAAGATTATAAATATAATATATAAGATGTTTCTCCTCGGTCTCACTT 30 0.75 No Hit GGTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACGTAGTAT 26 0.65 No Hit GTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCA 26 0.65 No Hit CTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCAT 26 0.65 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 25 0.625 No Hit GGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAAT 25 0.625 No Hit GCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAAAATGACCAAC 23 0.575 No Hit GTTTCTCCTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACAC 22 0.5499999999999999 No Hit GCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATG 22 0.5499999999999999 No Hit GTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATA 21 0.525 No Hit CTGAAGATTATAAATATAATATATAAGATGTTTCTCCTCGGTCTCACTTC 21 0.525 No Hit GGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGG 20 0.5 No Hit TAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAG 20 0.5 No Hit CTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCA 20 0.5 No Hit GCCGGCACGAGCACTGCCCCTTCCCTGAGCCATATCGACCGGCCGGGACC 19 0.475 No Hit CTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGACCT 18 0.44999999999999996 No Hit GCCCCTTCCCTGAGCCATATCGACCGGCCGGGACCTTTGGTTCAGGAAGA 18 0.44999999999999996 No Hit CTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTA 17 0.42500000000000004 No Hit GTCGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCC 17 0.42500000000000004 No Hit GGGTAATAATTCTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAA 17 0.42500000000000004 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 17 0.42500000000000004 No Hit CCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAA 17 0.42500000000000004 No Hit GTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAG 16 0.4 No Hit CCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGG 16 0.4 No Hit GTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGC 16 0.4 No Hit GTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCA 16 0.4 No Hit CCCTGAGCCATATCGACCGGCCGGGACCTTTGGTTCAGGAAGAGAAAAAA 15 0.375 No Hit GGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGT 15 0.375 No Hit GCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGA 15 0.375 No Hit CCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATAC 15 0.375 No Hit CACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCT 14 0.35000000000000003 No Hit GCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGT 14 0.35000000000000003 No Hit GTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCA 14 0.35000000000000003 No Hit GCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGG 14 0.35000000000000003 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 14 0.35000000000000003 No Hit CCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCA 14 0.35000000000000003 No Hit AGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAGCACT 13 0.325 No Hit GGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTCAG 13 0.325 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 12 0.3 No Hit GCCAACACTAATTCTAACTAGTTTCAGAAGGATGGTAGAAAGCATGGAGT 12 0.3 No Hit GCCCTTAAAATGTTTTAGAACATGAACACTTGTAGGAGATGATTGGATGG 12 0.3 No Hit GGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATA 12 0.3 No Hit GGCAACCATTTTTACGAATTGCTTCATGTGTAGGCAGAGGAAATGGGGGC 12 0.3 No Hit TTCTCCTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAA 12 0.3 No Hit CTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTGCTCATTAGCATAGA 11 0.27499999999999997 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 11 0.27499999999999997 No Hit CTTCCCTGAGCCATATCGACCGGCCGGGACCTTTGGTTCAGGAAGAGAAA 11 0.27499999999999997 No Hit CTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCCT 11 0.27499999999999997 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 11 0.27499999999999997 No Hit GCCAGTGGATCTTTTTCAATTGTACTGGGTCTCAAAGGGTTTGGTGTGGG 11 0.27499999999999997 No Hit CTTTGGATGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTAT 11 0.27499999999999997 No Hit CAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGAT 10 0.25 No Hit GCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAG 10 0.25 No Hit GTTAGGTGGTAGCTCTCCACTTAAACCACAACTATCAATATACCTGGAAT 10 0.25 No Hit CCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGA 10 0.25 No Hit AGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTT 10 0.25 No Hit CCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAAC 10 0.25 No Hit AGCCAGTGGATCTTTTTCAATTGTACTGGGTCTCAAAGGGTTTGGTGTGG 10 0.25 No Hit CCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGACAG 10 0.25 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 10 0.25 No Hit GGGGCAACCATTTTTACGAATTGCTTCATGTGTAGGCAGAGGAAATGGGG 10 0.25 No Hit CATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGC 9 0.22499999999999998 No Hit GGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGC 9 0.22499999999999998 No Hit TCCTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACGT 9 0.22499999999999998 No Hit GACCACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAG 9 0.22499999999999998 No Hit GCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAGCACTGCCCCTTCCCTG 9 0.22499999999999998 No Hit AGGTAGTTGGACTTGATAAGACACATTCCCAACTTCAAGAGTTCTCTTAA 9 0.22499999999999998 No Hit CTGCGCGTTGCCGCCGCCCATGGCTTCTTTCTCCTCGGCGAGAACGAGAG 9 0.22499999999999998 No Hit GAAGATTATAAATATAATATATAAGATGTTTCTCCTCGGTCTCACTTCTA 9 0.22499999999999998 No Hit CTCTTATCCTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGA 9 0.22499999999999998 No Hit GTAAGGTAGCCAACACTAATTCTAACTAGTTTCAGAAGGATGGTAGAAAG 9 0.22499999999999998 No Hit GACAAAGTAGAGAAAACGACTCGAGTTAGGGTTTGTACAGCGCCATTCGC 9 0.22499999999999998 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 9 0.22499999999999998 No Hit GCTTGAAGACAATGCTATCCCATATAAGCCTCTCGGGTGCCCACGATCCA 9 0.22499999999999998 No Hit GCCTGAAGATTATAAATATAATATATAAGATGTTTCTCCTCGGTCTCACT 9 0.22499999999999998 No Hit CGGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAAATA 8 0.2 No Hit TGAAGATTATAAATATAATATATAAGATGTTTCTCCTCGGTCTCACTTCT 8 0.2 No Hit GTGGAGGAGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGC 8 0.2 No Hit GTTCTTTGGATGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACAT 8 0.2 No Hit GGATGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTATCCGG 8 0.2 No Hit GTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACGTAG 8 0.2 No Hit GCTCAAAAGCCAAGGGGCAACCATTTTTACGAATTGCTTCATGTGTAGGC 8 0.2 No Hit GATCGAACCTCCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAA 8 0.2 No Hit CTTCTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACT 8 0.2 No Hit AGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCA 8 0.2 No Hit CTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACGCTCACTTAA 8 0.2 No Hit ACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCA 8 0.2 No Hit CAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCA 8 0.2 No Hit CTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACA 8 0.2 No Hit CTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTACTGGGTCTCAAA 8 0.2 No Hit GAACAGAGGTGCAAAATGTTCATCTTTGGCATGTTGTTCGAAATATGAAA 8 0.2 No Hit CATGGAGTTAGCAATTGCGCTTCTGATGCCGGAGAAGCTGATCGGTGCGG 8 0.2 No Hit GCCGCCGGCACGAGCACTGCCCCTTCCCTGAGCCATATCGACCGGCCGGG 8 0.2 No Hit GCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGT 8 0.2 No Hit TGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAG 8 0.2 No Hit CTCTTGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 8 0.2 No Hit GCGCGTTGCCGCCGCCCATGGCTTCTTTCTCCTCGGCGAGAACGAGAGGA 7 0.17500000000000002 No Hit GTCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTAT 7 0.17500000000000002 No Hit GCTAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCTCAAA 7 0.17500000000000002 No Hit GAATGAAAAGGCTGGGATCTATGAGCTTCTGAACAATTGGATGTTCTGCA 7 0.17500000000000002 No Hit CGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAG 7 0.17500000000000002 No Hit CTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAA 7 0.17500000000000002 No Hit CGCTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGG 7 0.17500000000000002 No Hit GCCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGC 7 0.17500000000000002 No Hit GGAATAATCACAAATATTTCAATGATATGTGTGGTCACAACAGAAAATAC 7 0.17500000000000002 No Hit CACATGTCTATGAATTTCACAGGTAGTTGGACTTGATAAGACACATTCCC 7 0.17500000000000002 No Hit GCCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 7 0.17500000000000002 No Hit CGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTTCTCATAATATC 7 0.17500000000000002 No Hit ATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATCTTTGG 7 0.17500000000000002 No Hit AAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTT 7 0.17500000000000002 No Hit ATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTACTGGGTCTCAAAGGG 7 0.17500000000000002 No Hit CTTCTTAGGTCAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGTT 7 0.17500000000000002 No Hit GCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCA 7 0.17500000000000002 No Hit GGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGA 7 0.17500000000000002 No Hit ATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGA 7 0.17500000000000002 No Hit CCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTG 7 0.17500000000000002 No Hit CTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTT 7 0.17500000000000002 No Hit CGTGGAGGAGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTG 7 0.17500000000000002 No Hit GATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATC 7 0.17500000000000002 No Hit GCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTT 7 0.17500000000000002 No Hit CTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 6 0.15 No Hit AGCATGAACAGTCTAGCATAATAGCCTGAAGATTATAAATATAATATATA 6 0.15 No Hit GGTGGGCATAAATGCATGATCATTTCTTGGTTCTTGCAGATCAAAAGTGA 6 0.15 No Hit GCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCA 6 0.15 No Hit ATTTGGAAATAGGAAGAACATCAAGATTCCCAAAATTCAGACATTATCGA 6 0.15 No Hit CAGTAAGGTAGCCAACACTAATTCTAACTAGTTTCAGAAGGATGGTAGAA 6 0.15 No Hit GGCTTCTTTCTCCTCGGCGAGAACGAGAGGAGAGGAGAGGAGAGGAGAGG 6 0.15 No Hit TTTTTTTTTTGCGATGAACAACCACAGGGGGTAATAATTCTCTTATCCTT 6 0.15 No Hit GTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAGTAATTTTTGCTTG 6 0.15 No Hit GCCGACTCGGCGAACTCGCCGTGGAGGAGCACGCAGAGGAGAACCAGCGC 6 0.15 No Hit CTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAG 6 0.15 No Hit CAAACGGAGGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAAT 6 0.15 No Hit CATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAA 6 0.15 No Hit CGCGCCGTTGCCGCCGGCACGAGCACTGCCCCTTCCCTGAGCCATATCGA 6 0.15 No Hit CGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGT 6 0.15 No Hit AGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTT 6 0.15 No Hit CGACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTC 6 0.15 No Hit CTATATAATACTTGTTTATGTTTGGTGGGCATAAATGCATGATCATTTCT 6 0.15 No Hit GGAGGCTGCAACCGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTC 6 0.15 No Hit CCCAACTTCAAGAGTTCTCTTAATAAGCTCCTTTGTTTCAGGATCGCAAG 6 0.15 No Hit CTCCACTTAAACCACAACTATCAATATACCTGGAATAAAAAAAAGAGAAA 6 0.15 No Hit GTTGGACTTGATAAGACACATTCCCAACTTCAAGAGTTCTCTTAATAAGC 6 0.15 No Hit AAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATT 6 0.15 No Hit CGGGCATCCTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTG 6 0.15 No Hit TGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCT 6 0.15 No Hit ACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAGCACTGCCCCT 6 0.15 No Hit CTCCTGTATTAACTCTTAAACATCTGACATCTCTATCTAGAAAAACAGAC 6 0.15 No Hit GTAGCCAACACTAATTCTAACTAGTTTCAGAAGGATGGTAGAAAGCATGG 6 0.15 No Hit TAGTTGCACTGTCGTGCCATCCGACCACCCAACCAAATTACGCTCAAAAG 5 0.125 No Hit CGGCCGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAA 5 0.125 No Hit ACCACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGC 5 0.125 No Hit GTTCTGTAGTTCAGGAAACAAAGTTCTCAGGTTCTTCTTCTCTGCTTGAC 5 0.125 No Hit CTACAGTCTACGGAATAATCACAAATATTTCAATGATATGTGTGGTCACA 5 0.125 No Hit ATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGG 5 0.125 No Hit TGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAG 5 0.125 No Hit ATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCC 5 0.125 No Hit TGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGT 5 0.125 No Hit CTTAGGATTTTTCTCTCTGTAAGTATGCTTGAGTTCTGAAAGCCTCGTGA 5 0.125 No Hit AGCATAATAGCCTGAAGATTATAAATATAATATATAAGATGTTTCTCCTC 5 0.125 No Hit GAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCA 5 0.125 No Hit CCGCCGACTCGGCGAACTCGCCGTGGAGGAGCACGCAGAGGAGAACCAGC 5 0.125 No Hit GGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCC 5 0.125 No Hit GAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAA 5 0.125 No Hit GGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTATCCGGTGAT 5 0.125 No Hit AGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGC 5 0.125 No Hit GTTGCCGCCGCCCATGGCTTCTTTCTCCTCGGCGAGAACGAGAGGAGAGG 5 0.125 No Hit GTTTGGTGGGCATAAATGCATGATCATTTCTTGGTTCTTGCAGATCAAAA 5 0.125 No Hit ACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGG 5 0.125 No Hit TTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCT 5 0.125 No Hit GTGGGCATAAATGCATGATCATTTCTTGGTTCTTGCAGATCAAAAGTGAA 5 0.125 No Hit CCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAG 5 0.125 No Hit GATGTTTCTCCTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTA 5 0.125 No Hit GTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGC 5 0.125 No Hit CTCATAATATCTGACAGGCAAACTTCAATAGATATAAGAAGTTAACTACC 5 0.125 No Hit CTCTTAAACATCTGACATCTCTATCTAGAAAAACAGACAGATAGCCTGGG 5 0.125 No Hit ACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTT 5 0.125 No Hit AGTTTCTCCTCGGTCTCACTTCTATGCAACTGGCAAACATGACCATTACA 5 0.125 No Hit GTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTG 5 0.125 No Hit CATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTACTG 5 0.125 No Hit CGTAGCGCCTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTG 5 0.125 No Hit GTCTAGCATAATAGCCTGAAGATTATAAATATAATATATAAGATGTTTCT 5 0.125 No Hit CAAACGTATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTG 5 0.125 No Hit ATCCATTCCTGACTCCTGATGAACAACCGGGAGAAAACTTCAACGGCTAT 5 0.125 No Hit TCACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGC 5 0.125 No Hit CACAAGCAAAACCATCCATTCCTGACTCCTGATGAACAGCCGGGAGAAAA 5 0.125 No Hit CATTCCTGACTCCTGATGAACAGCCGGGAGAAAACTTCAACGGCTATACG 5 0.125 No Hit TTTCACAGCCACTCAGACTCAACTTGTGGATCTCCATATCTTCAAAGTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGAGG 10 0.007690679 139.3625 7 AGAGGTG 10 0.007690679 139.3625 9 TCGAACA 10 0.007690679 139.3625 3 TTCGAAC 10 0.007690679 139.3625 2 CAGAGGT 10 0.007690679 139.3625 8 CGAACAG 10 0.007690679 139.3625 4 GAACAGA 10 0.007690679 139.3625 5 AACAGAG 10 0.007690679 139.3625 6 CCTCGGT 50 2.1619181E-4 55.745003 1 CTCGGTC 55 3.4623445E-4 50.677273 2 TCGGTCT 60 5.319623E-4 46.454166 3 CGGTCTC 60 5.319623E-4 46.454166 4 GTCTCAC 65 7.893315E-4 42.880768 6 TCTCACT 65 7.893315E-4 42.880768 7 TCACTTC 70 0.0011369749 39.81786 9 GTGCCAC 30 1.9056935E-5 32.50437 140-144 ACTGTGC 40 4.070711E-6 28.441328 140-144 CTGTGCC 40 4.070711E-6 28.441328 140-144 TGTGCCA 30 7.3761836E-4 27.086977 140-144 TAGTAGA 50 1.202734E-6 24.706924 120-124 >>END_MODULE ERR5262812 read2 length is 60-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262812_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 60-150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.204 37.0 37.0 37.0 37.0 37.0 2 36.023 37.0 37.0 37.0 37.0 37.0 3 36.0175 37.0 37.0 37.0 37.0 37.0 4 36.101 37.0 37.0 37.0 37.0 37.0 5 36.214 37.0 37.0 37.0 37.0 37.0 6 36.302 37.0 37.0 37.0 37.0 37.0 7 36.187 37.0 37.0 37.0 37.0 37.0 8 36.2025 37.0 37.0 37.0 37.0 37.0 9 36.2205 37.0 37.0 37.0 37.0 37.0 10-14 36.248000000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.2109 37.0 37.0 37.0 37.0 37.0 20-24 36.259100000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.167199999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.200700000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.1594 37.0 37.0 37.0 37.0 37.0 40-44 36.0873 37.0 37.0 37.0 37.0 37.0 45-49 36.05669999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.070299999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.973800000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.911284121030256 37.0 37.0 37.0 37.0 37.0 65-69 36.0048512128032 37.0 37.0 37.0 37.0 37.0 70-74 35.88082020505126 37.0 37.0 37.0 37.0 37.0 75-79 35.91395697848924 37.0 37.0 37.0 37.0 37.0 80-84 35.81370046060324 37.0 37.0 37.0 37.0 37.0 85-89 35.81811772226195 37.0 37.0 37.0 37.0 37.0 90-94 35.79761905288724 37.0 37.0 37.0 37.0 37.0 95-99 35.72689873789407 37.0 37.0 37.0 37.0 37.0 100-104 35.67191144642282 37.0 37.0 37.0 37.0 37.0 105-109 35.641470371041855 37.0 37.0 37.0 37.0 37.0 110-114 35.57754496525199 37.0 37.0 37.0 37.0 37.0 115-119 35.50012037454147 37.0 37.0 37.0 37.0 37.0 120-124 35.51655357508353 37.0 37.0 37.0 37.0 37.0 125-129 35.47550652946522 37.0 37.0 37.0 37.0 37.0 130-134 35.410458657727865 37.0 37.0 37.0 34.6 37.0 135-139 35.32238152870851 37.0 37.0 37.0 32.2 37.0 140-144 35.18971012575033 37.0 37.0 37.0 29.8 37.0 145-149 35.2824482688264 37.0 37.0 37.0 32.2 37.0 150 35.19922225545917 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 3.0 15 3.0 16 0.0 17 1.0 18 1.0 19 1.0 20 1.0 21 4.0 22 14.0 23 9.0 24 10.0 25 7.0 26 9.0 27 13.0 28 11.0 29 20.0 30 22.0 31 27.0 32 49.0 33 105.0 34 221.0 35 529.0 36 2631.0 37 308.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.675000000000004 23.05 8.125 25.15 2 27.175 24.8 27.55 20.474999999999998 3 18.625 28.225 32.75 20.4 4 23.599999999999998 30.349999999999998 21.0 25.05 5 23.5 35.825 19.975 20.7 6 22.125 35.775 20.95 21.15 7 20.724999999999998 20.325 36.525 22.425 8 19.425 23.925 29.675 26.974999999999998 9 18.275 23.025000000000002 28.549999999999997 30.15 10-14 22.134999999999998 27.134999999999998 25.814999999999998 24.915000000000003 15-19 22.55 26.790000000000003 27.195000000000004 23.465 20-24 21.310000000000002 27.115000000000002 28.444999999999997 23.13 25-29 23.01 25.240000000000002 28.515 23.235 30-34 23.494999999999997 27.715 26.900000000000002 21.89 35-39 22.605 28.34 27.205000000000002 21.85 40-44 21.84 28.365000000000002 27.0 22.795 45-49 22.23 26.99 27.845 22.935 50-54 20.419999999999998 27.810000000000002 28.58 23.189999999999998 55-59 21.165 26.0 28.4 24.435000000000002 60-64 22.534506901380276 26.615323064612923 27.920584116823367 22.929585917183438 65-69 23.220805201300326 26.691672918229557 27.321830457614404 22.765691422855713 70-74 24.281070267566893 27.04176044011003 26.16654163540885 22.510627656914227 75-79 23.261630815407706 28.344172086043024 25.23261630815408 23.1615807903952 80-84 23.45258944208156 26.53490117588191 27.72579434575932 22.286715036277208 85-89 24.78816746051642 27.901729756831283 26.89897217347706 20.41113060917523 90-94 23.73988642645359 27.564199205990253 27.30287954168551 21.393034825870647 95-99 25.398506860371267 26.926957223567392 26.533494753833736 21.1410411622276 100-104 25.399898115129904 26.016301579215483 26.12328069281712 22.460519612837494 105-109 24.951076320939332 27.386960552065094 27.06766917293233 20.59429395406324 110-114 23.019322406660734 27.06184217416348 27.758286641880925 22.160548777294863 115-119 23.90529396115988 27.188081936685286 28.326682628358608 20.57994147379622 120-124 23.923805424020664 26.791863969005597 27.685105467068443 21.599225139905297 125-129 24.091851205410713 28.138976764481292 26.84629649830915 20.922875531798844 130-134 25.301137940605052 27.62142658895365 26.738828753816264 20.338606716625033 135-139 23.29992639148406 26.20463167431063 28.667685861502747 21.827756072702563 140-144 23.162994578382744 27.644480332218247 28.019379397854422 21.173145691544583 145-149 23.55505803334708 28.15059211689153 28.462852766158015 19.83149708360337 150 25.037391564463057 24.618606042476816 30.002991325157048 20.341011067903082 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 2.0 18 2.5 19 1.0 20 1.0 21 0.5 22 1.0 23 2.5 24 3.0 25 2.5 26 2.0 27 1.5 28 1.0 29 20.0 30 32.0 31 50.5 32 81.0 33 67.0 34 74.0 35 88.0 36 101.0 37 100.0 38 113.5 39 165.0 40 150.5 41 138.0 42 171.0 43 176.5 44 204.0 45 227.0 46 176.5 47 172.5 48 180.5 49 150.5 50 170.5 51 209.5 52 188.0 53 104.5 54 58.0 55 64.5 56 62.5 57 75.5 58 66.5 59 36.5 60 29.0 61 24.0 62 30.5 63 36.0 64 25.0 65 20.0 66 18.0 67 19.0 68 23.5 69 18.0 70 13.0 71 11.5 72 7.5 73 7.0 74 9.5 75 6.5 76 3.5 77 3.0 78 1.0 79 0.0 80 0.0 81 1.5 82 1.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 60-61 1.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 1.0 84-85 5.0 86-87 5.0 88-89 4.0 90-91 0.0 92-93 10.0 94-95 3.0 96-97 7.0 98-99 21.0 100-101 16.0 102-103 13.0 104-105 16.0 106-107 21.0 108-109 26.0 110-111 31.0 112-113 25.0 114-115 27.0 116-117 16.0 118-119 16.0 120-121 18.0 122-123 19.0 124-125 15.0 126-127 26.0 128-129 31.0 130-131 26.0 132-133 15.0 134-135 33.0 136-137 35.0 138-139 25.0 140-141 22.0 142-143 29.0 144-145 28.0 146-147 32.0 148-149 37.0 150-151 3343.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 50.790861159929705 21.675 2 20.796719390743995 17.75 3 12.595196250732279 16.125 4 5.799648506151142 9.9 5 3.7492677211482133 8.0 6 2.2261277094317515 5.7 7 1.1130638547158758 3.325 8 0.5858230814294083 2.0 9 0.35149384885764495 1.35 >10 1.9917984768599881 14.174999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAAAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCC 42 1.05 No Hit GTTCATTTCACAAGAAGTCCGCAGTCCACAACAATATCCTCATAGCTAAA 41 1.0250000000000001 No Hit GTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGA 32 0.8 No Hit GCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCC 30 0.75 No Hit CTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTTC 28 0.7000000000000001 No Hit CAACAATATCCTCATAGCTAAATATTTTGTGAGATGAAACTTAAGGAAAC 22 0.5499999999999999 No Hit CCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTT 21 0.525 No Hit GCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGAACAGCCATGCTGGTG 20 0.5 No Hit AAAATTTCTCCAGCCGAAAAACTTCCGCCGAGGAAGAGCATCCTCTCCTC 19 0.475 No Hit GTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTT 19 0.475 No Hit GGCACAGTATATCTTATTCTTCTACTAAGTGAGGTTATCCCTAGATGAAT 18 0.44999999999999996 No Hit AGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACC 18 0.44999999999999996 No Hit CGCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAA 17 0.42500000000000004 No Hit GTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTGCTCAGATA 15 0.375 No Hit GGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAAC 15 0.375 No Hit GGATGATGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTT 14 0.35000000000000003 No Hit AGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAG 14 0.35000000000000003 No Hit GTCCAAAACCTCGGCAGTCTTCATCCCTCACGGGCCAGGTGCTGTCAAAG 12 0.3 No Hit CCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTT 12 0.3 No Hit GTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCC 12 0.3 No Hit GGCCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATG 12 0.3 No Hit AGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCT 12 0.3 No Hit GCACAGTATATCTTATTCTTCTACTAAGTGAGGTTATCCCTAGATGAATG 11 0.27499999999999997 No Hit GTTTCTGAGTTTTGTGGAAGTCTTTTTGCCTAGCTTGTTTCTGTACTTCT 11 0.27499999999999997 No Hit GGGTGCTCTCGAAAAAACAAAGGTTATTTGAAGGTTGGCATGCCCTTTGG 10 0.25 No Hit TGTAGAGATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTTTATGGG 10 0.25 No Hit CAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATT 10 0.25 No Hit GTCCACAACAATATCCTCATAGCTAAATATTTTGTGAGATGAAACTTAAG 10 0.25 No Hit CAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCT 10 0.25 No Hit CTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTT 10 0.25 No Hit CTTGCAAACACCCTTTTTTCTCTTCCTGAACCAAAGGTCCCGGCCGGTCG 10 0.25 No Hit AGCTATCAAGCAGGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGA 10 0.25 No Hit GTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTACAT 10 0.25 No Hit GCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTTGTGGA 10 0.25 No Hit GCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAAC 9 0.22499999999999998 No Hit CTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACA 9 0.22499999999999998 No Hit TGGGAACCCTAAAGATGCCAACAAAGCGCAATGCTCGCCAAAGAATGGTG 9 0.22499999999999998 No Hit ATTTGACTTCATTGTTCAGAAGAGAGGGGTTCACCGCTTCTGCTTCACCA 9 0.22499999999999998 No Hit CTTAAGGAAACAATACTATTATTTTGTGAGTGGCACAGTATATCTTATTC 9 0.22499999999999998 No Hit CCTAGATGAATGTTTATAGACTTCCATTGTTGCATCACTAGTTTCTGTAA 9 0.22499999999999998 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 8 0.2 No Hit CATCAGGCCTCTTCCAATCCTTTTGCCGGCTCGTACATGCCCAGCAGCGG 8 0.2 No Hit TGCGGTTGAGGAGTTGGGCAAAACATCTCATACAGATCCAAGATTACCAT 8 0.2 No Hit AGCAGAGGCCGACCGGCGACCCAAGGAGTTCTCTATCTGACAAAAGAAAT 8 0.2 No Hit GGTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCAT 8 0.2 No Hit AACTTAAGGAAACAATACTATTATTTTGTGAGTGGCACAGTATATCTTAT 8 0.2 No Hit GTGCTTTTAAGTTCGCCCTGGATGCTGACTTCATTCTGACTTACACTGAT 8 0.2 No Hit ATTAGATTCTCTGTGTGATGATGGACATCTGGACTGCAATAATATGCTGT 8 0.2 No Hit GGAAACAATACTATTATTTTGTGAGTGGCACAGTATATCTTATTCTTCTA 8 0.2 No Hit CTTCACAGATAAGAGATGGTCAGCTCCAGGCCAAGATGGGTTAAAATTGC 8 0.2 No Hit GGAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGACCA 7 0.17500000000000002 No Hit GTCAGCTCCAGGCCAAGATGGGTTAAAATTGCCGGAAAATGGTGTTCATT 7 0.17500000000000002 No Hit ATCAGGCCTCTTCCAATCCTTTTGCCGGCTCGTACATGCCCAGCAGCGGC 7 0.17500000000000002 No Hit GGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTG 7 0.17500000000000002 No Hit CTATTACTTGGAGTATGAACCTCATCCATCATCAACTAAAAACTTGCATC 7 0.17500000000000002 No Hit CTCGCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCA 7 0.17500000000000002 No Hit GGACGATCCATTACCAGGCATAGAAGGTCTTCGAATAACGGGTGAAGCTT 7 0.17500000000000002 No Hit CTTATGGTCAGCTCCATGCCCGCAACACGTACACTGGAACGGGGATGATG 7 0.17500000000000002 No Hit CTCATAGCTAAATATTTTGTGAGATGAAACTTAAGGAAACAATACTATTA 7 0.17500000000000002 No Hit CCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTC 7 0.17500000000000002 No Hit AAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGC 7 0.17500000000000002 No Hit CTATCATTGACATGGCTCACAAAATTTATACACTAGCTGTTGACCATAAT 7 0.17500000000000002 No Hit GTTAAAATTGCCGGAAAATGGTGTTCATTTCACAAGAAGTCCGCAGTCCA 7 0.17500000000000002 No Hit GGGAGGAGTGCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCAT 7 0.17500000000000002 No Hit GCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAAACCC 7 0.17500000000000002 No Hit GGAAAATTGTTGCTTCAATGCATCACGGATTGACTATTACTTGGAGTATG 7 0.17500000000000002 No Hit ACCAGACTTATGTTATTGCCACATCCACAAAGGTTGACATCTCTGGTGTT 7 0.17500000000000002 No Hit CCAAGATGGGTTAAAATTGCCGGAAAATGGTGTTCATTTCACAAGAAGTC 7 0.17500000000000002 No Hit AGTATATCTTATTCTTCTACTAAGTGAGGTTATCCCTAGATGAATGTTTA 7 0.17500000000000002 No Hit GAAGCTTTAAAACCCACACCAAACCCTTTGAGACCCAGTACAATTGAAAA 6 0.15 No Hit GGTTAAAATTGCCGGAAAATGGTGTTCATTTCACAAGAAGTCCGCAGTCC 6 0.15 No Hit GCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTTCCTGAACCAAAGG 6 0.15 No Hit AGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCA 6 0.15 No Hit GATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGA 6 0.15 No Hit CACGTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTA 6 0.15 No Hit GACTGATACATCAAATAAGATAAAGAAGGGGACAAAAGGAGATGTTTCTG 6 0.15 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCGGCTCTGACTATGACTG 6 0.15 No Hit GGACATGGTCTTGGTGACCCAATATTTTGACACTATGAAGGATATCGGGG 6 0.15 No Hit AGATGGTCAGCTCCAGGCCAAGATGGGTTAAAATTGCCGGAAAATGGTGT 6 0.15 No Hit GAAACTTAAGGAAACAATACTATTATTTTGTGAGTGGCACAGTATATCTT 6 0.15 No Hit GGTCAGTTGCAGCAAGAGCTAAACACATTGTACTGCCGAGAAGAAATAGA 6 0.15 No Hit AAGGAGACTACCAAGAATCTGCCTGACTTCAAGAAGGATGACCAGAAGAC 6 0.15 No Hit GCAGCAGCAGCAGCAGCAGGCCTTCATGCTCCAGCAGCAGCAGCAGCAGC 6 0.15 No Hit GTGAGATGAAACTTAAGGAAACAATACTATTATTTTGTGAGTGGCACAGT 6 0.15 No Hit CTTATTCTTCTACTAAGTGAGGTTATCCCTAGATGAATGTTTATAGACTT 6 0.15 No Hit AATAAAGGAGATAGAAGATGTGATAGGTAAGGGCAATCTAGGAAAACATC 6 0.15 No Hit TAAAATTGCCGGAAAATGGTGTTCATTTCACAAGAAGTCCGCAGTCCACA 6 0.15 No Hit ATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAA 6 0.15 No Hit GTTCAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAAAAAGCTGTTTGATG 6 0.15 No Hit GCAGCTATCAAGCAGGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAAA 6 0.15 No Hit GATGATGATGACTGGACAACAGTCTGTTCATCATCAGGCCTCTTCCAATC 6 0.15 No Hit GGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGAC 6 0.15 No Hit GTTGACATCTCTGGTGTTAAGGTGGAGAAGTTTGATGACAAGTACTTTGC 6 0.15 No Hit AGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAATTT 6 0.15 No Hit AAATATTTTGTGAGATGAAACTTAAGGAAACAATACTATTATTTTGTGAG 6 0.15 No Hit GTGGGTGCGCCATTTGGAAGATGGCTCCGTAAAATTCATAGAAGGAGCAA 6 0.15 No Hit GCCCGCAACACGTACACTGGAACGGGGATGATGTAGAGATGCTTGGTTTT 6 0.15 No Hit AGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACCCA 6 0.15 No Hit GGACGGGGATGTTGTCATGCTGGATGATGACGAAGACTTGCATGATGCAG 6 0.15 No Hit CAGCAGATGATGATGATGACTGGACAACAGTCTGTTCATCATCAGGCCTC 6 0.15 No Hit ATTTTGTGAGTGGCACAGTATATCTTATTCTTCTACTAAGTGAGGTTATC 6 0.15 No Hit AGTTGATATTTGCTTTGGTGATCCCAATGGTATCGAGAATCACTGCTTAC 6 0.15 No Hit ACAGTCTGTTCATCATCAGGCCTCTTCCAATCCTTTTGCCGGCTCGTACA 6 0.15 No Hit GCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCG 6 0.15 No Hit GACGAAGACTTGCATGATGCAGCTATCAAGCAGGAGCTGAATCCTCTGAG 6 0.15 No Hit ACAACAATATCCTCATAGCTAAATATTTTGTGAGATGAAACTTAAGGAAA 6 0.15 No Hit GACACACTTAACCAACTGAAACACTTGACAACATTATCTTTCCATGCGGT 6 0.15 No Hit CCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTTCCTGAACC 5 0.125 No Hit CAAACCCTTTGAGACCCAGTACAATTGAAAAAGATCCACTGGCTCATTTG 5 0.125 No Hit ACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGAGACTGTCATGTTA 5 0.125 No Hit GTTATCCCTAGATGAATGTTTATAGACTTCCATTGTTGCATCACTAGTTT 5 0.125 No Hit GGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGAACAGCCATGCTG 5 0.125 No Hit GCTCGGCTCTCTCGACTGCGTCCTCTTCCTCGCCGAGAAGGGGAACGGAA 5 0.125 No Hit GTACAACAAAGCAAATAGTCCAAATCCACGCGCGGCCTGGAATAATGTTT 5 0.125 No Hit CAGATTCCAGCGGAATTGCAAAATCTAACTTACCTGACAAACCTGTATGA 5 0.125 No Hit ACTCGCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGC 5 0.125 No Hit GCTAAAATTAGTTAACATACCAAACTTGAAAAGATGGGTAAAAAATGACA 5 0.125 No Hit CGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGAGGGGT 5 0.125 No Hit GTTCTCTATCTGACAAAAGAAATGTACATGTTCTCATTTTCAAAGAGGCG 5 0.125 No Hit GCTAAATATTTTGTGAGATGAAACTTAAGGAAACAATACTATTATTTTGT 5 0.125 No Hit GATGACACCTGGGAGCCAATTGATGGATTAGGGAACTGCAATGAGGCAAT 5 0.125 No Hit CAACAAAGCCTAGTATGTCTGGATCAGAGAGGTCTAAGCGTACAATTCCT 5 0.125 No Hit AGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTT 5 0.125 No Hit GTCTGTTCATCATCAGGCCTCTTCCAATCCTTTTGCCGGCTCGTACATGC 5 0.125 No Hit GATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAAACCCTTTGA 5 0.125 No Hit CAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGC 5 0.125 No Hit GGGGAAAATTGTTGCTTCAATGCATCACGGATTGACTATTACTTGGAGTA 5 0.125 No Hit GTCAAAGATTGGTGCTGATGATTGAACTTGGGAAGTTAAATCTAAAGGCT 5 0.125 No Hit GTTTATAGACTTCCATTGTTGCATCACTAGTTTCTGTAAAAATAATACTA 5 0.125 No Hit CCATCGTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAA 5 0.125 No Hit GGACCATTCAAGATCAATGGAGTGCCAATTCGCCGCGTCAACCAGACTTA 5 0.125 No Hit CGGTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGT 5 0.125 No Hit GATCATTACTTGTTAAAATTTCAGAATTGCAATCTAGAATGATTACTTTG 5 0.125 No Hit AAACAATACTATTATTTTGTGAGTGGCACAGTATATCTTATTCTTCTACT 5 0.125 No Hit CCTGGGATCAGCAAGCCGCCCCAGTGGCATCATCAGCTCCGATGGCAATG 5 0.125 No Hit GGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGC 5 0.125 No Hit GGGTGCGCCCCTCTGCATCCGACCTGGGAACCACGGGGCCGACCAAGGCC 5 0.125 No Hit TATCAAGCAGGAGCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGAACA 5 0.125 No Hit GCTGTCTTCGATAACAGGGGAAAATTGTTGCTTCAATGCATCACGGATTG 5 0.125 No Hit ATCAGCTCCGATGGCAATGACGATGATGCAGCAGCAGCAGCCGATGAACA 5 0.125 No Hit AGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTT 5 0.125 No Hit AGTTGCTATGCGTTACCAAGCCAAACTAGGGATCATGGCTGCTGGATGTT 5 0.125 No Hit CTCAGATAAAATCAGTTATTGATGAAGCTTTAAAACCCACACCAAACCCT 5 0.125 No Hit CAAGAAGGTTGCTGAGAAGGGCGGCATGAAAAAAAAGCTGTTTGATGTTG 5 0.125 No Hit GTTATTGATGAAGCTTTAAAACCCACACCAAACCCTTTGAGACCCAGTAC 5 0.125 No Hit CATCAAGCAGTGATACCGCAGTTATTATGTATACAAGTGGAAGTACAGGT 5 0.125 No Hit GCTTCTCGCATAACGTGGAGTACGAGGGGGACACTGTCCATGTATCCTTC 5 0.125 No Hit GCAGGCCTTCATGCTCCAGCAGCAGCAGCAGCAGCAGTATCAGCAGCAGT 5 0.125 No Hit CTGGACAACAGTCTGTTCATCATCAGGCCTCTTCCAATCCTTTTGCCGGC 5 0.125 No Hit CATGGACATGGTCTTGGTGACCCAATATTTTGACACTATGAAGGATATCG 5 0.125 No Hit CAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCC 5 0.125 No Hit CTCCAGGCCAAGATGGGTTAAAATTGCCGGAAAATGGTGTTCATTTCACA 5 0.125 No Hit GAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCA 5 0.125 No Hit GTCTCTATGGCTTATTCATGCTTGAATTTGAAGAGCATAGTGAAACAAAT 5 0.125 No Hit AGAGAATTCGATGCCCAATTTAAGTGTGAGGAAAACAAGGAGATCATTTT 5 0.125 No Hit ATTGGATATGGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAA 5 0.125 No Hit ATCGCATAAGAGACGCCGTGTTCAAGAAGGTTGCTGAGAAGGGCGGCATG 5 0.125 No Hit GATGCTTGGTTTTTTGCATTACATTTTTTCAATTTTTTTATGGGAATTGG 5 0.125 No Hit AGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAGTTCCTGCAA 5 0.125 No Hit GACCAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCGTCCC 5 0.125 No Hit CTTGTGAGTCCTTAACTTGCTATGCTCTTTTCAAAATTACAAGAGCTCTG 5 0.125 No Hit GTTCCAAAGATGAACTTGGATGATGTATTTGAGCAGAAGAATGAAATCGC 5 0.125 No Hit GGGATGTTGTCATGCTGGATGATGACGAAGACTTGCATGATGCAGCTATC 5 0.125 No Hit GCATTGGGAACCCTAAAGATGCCAACAAAGCGCAATGCTCGCCAAAGAAT 5 0.125 No Hit AACAACACTCGCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGC 5 0.125 No Hit GGGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCT 5 0.125 No Hit GCTTGGGAACTATGTCTGCCTCAATCATTGGCATATTAGGTGTGCTGTTT 5 0.125 No Hit GACACGCCGTGGCATTATAGCGAGGATGGGGTGGATCTTGTTGTTAAAGA 5 0.125 No Hit CTCACGGGCCAGGTGCTGTCAAAGATATCGCTTCACAGATAAGAGATGGT 5 0.125 No Hit GGATGACCAGAAGACCATTGACGCTGAGTTGATCAAGGCTATTGAGGCCG 5 0.125 No Hit GAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAAGTCCTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGATGC 10 0.007690679 139.3625 8 AATTCGA 10 0.007690679 139.3625 5 TTCGATG 10 0.007690679 139.3625 7 AGAATTC 10 0.007690679 139.3625 3 ATTCGAT 10 0.007690679 139.3625 6 GAATTCG 10 0.007690679 139.3625 4 CGATGCC 10 0.007690679 139.3625 9 >>END_MODULE Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra Read 2009834 spots for ERR5262812.sra Written 2009834 spots for ERR5262812.sra Read 2009817 spots for ERR5262812.sra Written 2009817 spots for ERR5262812.sra SRR ids: ['ERR5262812.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bf94r384 ERR5262812.sra spots: 40196357 blocks: [[1, 2009817], [2009818, 4019634], [4019635, 6029451], [6029452, 8039268], [8039269, 10049085], [10049086, 12058902], [12058903, 14068719], [14068720, 16078536], [16078537, 18088353], [18088354, 20098170], [20098171, 22107987], [22107988, 24117804], [24117805, 26127621], [26127622, 28137438], [28137439, 30147255], [30147256, 32157072], [32157073, 34166889], [34166890, 36176706], [36176707, 38186523], [38186524, 40196357]] ERR5262812 file size 13190922 ERR5262812 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262812 ERR5262812_1.fastq ERR5262812_2.fastq Input file: ERR5262812_1.fastq Paired file: ERR5262812_2.fastq trimmed: ERR5262812-trimmed-pair1.fastq, ERR5262812-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:27:52 2024 >> started Fri Dec 6 12:28:44 2024 >> done (51.107s) 40196357 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 40196356 (100.00%) read pairs available; of these: 12115 ( 0.03%) trimmed read pairs available after processing 40184241 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 2 0.00% 24 1 0.00% 25 5 0.00% 26 3 0.00% 27 5 0.00% 28 4 0.00% 29 2 0.00% 30 1 0.00% 31 2 0.00% 32 7 0.00% 33 5 0.00% 34 6 0.00% 35 5 0.00% 36 3 0.00% 37 5 0.00% 38 9 0.00% 39 5 0.00% 40 1 0.00% 41 5 0.00% 42 6 0.00% 43 2 0.00% 44 9 0.00% 45 7 0.00% 46 2 0.00% 47 6 0.00% 48 2 0.00% 49 370 0.00% 50 434 0.00% 51 504 0.00% 52 528 0.00% 53 546 0.00% 54 584 0.00% 55 661 0.00% 56 762 0.00% 57 851 0.00% 58 1054 0.00% 59 1106 0.00% 60 1274 0.00% 61 1503 0.00% 62 1608 0.00% 63 1775 0.00% 64 2061 0.01% 65 2107 0.01% 66 2402 0.01% 67 2749 0.01% 68 3061 0.01% 69 3513 0.01% 70 4186 0.01% 71 4552 0.01% 72 5275 0.01% 73 5936 0.01% 74 6611 0.02% 75 7346 0.02% 76 8160 0.02% 77 8700 0.02% 78 9889 0.02% 79 11135 0.03% 80 12538 0.03% 81 13941 0.03% 82 15717 0.04% 83 17255 0.04% 84 19339 0.05% 85 20770 0.05% 86 22603 0.06% 87 24169 0.06% 88 26097 0.06% 89 27939 0.07% 90 30362 0.08% 91 33052 0.08% 92 36010 0.09% 93 38874 0.10% 94 41851 0.10% 95 43834 0.11% 96 46275 0.12% 97 48730 0.12% 98 50177 0.12% 99 53224 0.13% 100 55444 0.14% 101 58275 0.14% 102 62541 0.16% 103 65538 0.16% 104 68580 0.17% 105 71594 0.18% 106 73338 0.18% 107 75800 0.19% 108 77017 0.19% 109 80001 0.20% 110 82284 0.20% 111 85269 0.21% 112 88701 0.22% 113 92776 0.23% 114 95463 0.24% 115 98182 0.24% 116 99687 0.25% 117 103107 0.26% 118 104122 0.26% 119 104728 0.26% 120 105821 0.26% 121 109734 0.27% 122 111981 0.28% 123 115938 0.29% 124 118788 0.30% 125 120990 0.30% 126 123547 0.31% 127 124691 0.31% 128 125360 0.31% 129 127292 0.32% 130 127957 0.32% 131 128706 0.32% 132 132695 0.33% 133 135448 0.34% 134 136435 0.34% 135 139954 0.35% 136 142311 0.35% 137 143067 0.36% 138 144215 0.36% 139 147403 0.37% 140 150259 0.37% 141 154818 0.39% 142 157624 0.39% 143 162259 0.40% 144 164951 0.41% 145 163197 0.41% 146 167791 0.42% 147 348378 0.87% 148 152525 0.38% 149 153273 0.38% 150 33492389 83.32% 40196356 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=11.48 fanout-score-rank=24 prefix-density=0.45 prefix-fanout=5.8 sequence=AGCTTCTTCAGGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=134.45 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=6.7 sequence=CCATCACAAATCAAATGTTTAGACAGACAGATAACACCAGATAAAGCCACTCATGGCAGATCACAGGATAACAGCAATAGCAGGCCTTGGGATTGCAACAGGATACAGGCTCTAGTACTCATCGCCCTCATCGCCTTCATCACCCTCGTCGAACTCTGCACCAACCTCTTCATAATCCTTCTCCAGGGCAGCCAAATCCTCACGGGCCTCAGAGAACTCTCCCTCCTCCATGCCCTCACCCACATACCAGTGGACAAAGGCACGCTTGGCGTACATGAGATCAAACTTGTGGTCAATGCGGGAGAAGACCTCAACAACACTGGTGGAGTTGGAGATCATGCACACTGCCCTCTGGACCTTGGCAAGGTCACCACCTGGGACAACGCTAGGTGGCTGGTAGTTGATGCCACACTTGAAGCCAGTGGGGCACCAGTCAACAAA criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=3.45 fanout-score-rank=29 prefix-density=0.32 prefix-fanout=2.9 sequence=AGCAGAGCAGAG criterion=fanout-score sequence-density=0.13 sequence-density-rank=9 fanout-score=95.89 fanout-score-rank=1 prefix-density=0.74 prefix-fanout=16.7 sequence=CGCCGCCGCCGC ERR5262812 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:29:12 Started mapping on | Dec 06 12:29:12 Finished on | Dec 06 12:31:53 Mapping speed, Million of reads per hour | 898.80 Number of input reads | 40196356 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 38536317 Uniquely mapped reads % | 95.87% Average mapped length | 290.53 Number of splices: Total | 35384509 Number of splices: Annotated (sjdb) | 32829221 Number of splices: GT/AG | 34878667 Number of splices: GC/AG | 420995 Number of splices: AT/AC | 20949 Number of splices: Non-canonical | 63898 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.34 Insertion rate per base | 0.01% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 391183 % of reads mapped to multiple loci | 0.97% Number of reads mapped to too many loci | 1261 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.13% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1268856 1268856 1268856 N_multimapping 391183 391183 391183 N_noFeature 1522454 37535547 1841786 N_ambiguous 812189 4934 130459 UnstrandedReadsAssigned:36201674 PositiveStrandReadsAssigned:995836 NegativeStrandReadsAssigned:36564072 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262812 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262812-trimmed-pair1.fastq ERR5262812-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 40,196,356 reads, 37,080,222 reads pseudoaligned [quant] estimated average fragment length: 262.362 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,214 rounds 52973 ERR5262812.ke.tsv 35125 ERR5262812.se.tsv 88098 total ==> ERR5262812.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 675.37 0 0 PNS24247 1044 782.638 200.788 10.0937 PNS24249 1928 1666.64 652.938 15.4136 PNS24246 1044 782.638 200.788 10.0937 PNS24248 1044 782.638 200.788 10.0937 PNS24244 1471 1209.64 210.698 6.85295 PNS24243 293 102.073 0 0 KQK14069 1603 1341.64 30524.5 895.129 KQK14071 474 240.864 993.01 162.201 ==> ERR5262812.se.tsv <== BRADI_1g14170v3 34871 BRADI_1g53295v3 190 BRADI_1g59795v3 997 BRADI_1g07683v3 0 BRADI_1g00485v3 23 BRADI_1g20270v3 1040 BRADI_1g74790v3 3257 BRADI_1g09890v3 0 BRADI_1g77505v3 278 BRADI_1g48960v3 0 ERR5262812 completed mapping pipeline successfully