Starting /dee2/code/volunteer_pipeline.sh ERR5262813 current disk space = 1551110213632 free memory = 1599910876 ERR5262813 SRAfilesize 38c72f41629d67b62dd108e6782233e7 ERR5262813.sra ERR5262813.sra file validated ERR5262813 is paired end ERR5262813 is conventional basespace ERR5262813 read1 length is 77-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262813_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 77-150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.675 37.0 37.0 37.0 37.0 37.0 2 36.321 37.0 37.0 37.0 37.0 37.0 3 36.616 37.0 37.0 37.0 37.0 37.0 4 36.6575 37.0 37.0 37.0 37.0 37.0 5 36.642 37.0 37.0 37.0 37.0 37.0 6 36.609 37.0 37.0 37.0 37.0 37.0 7 36.612 37.0 37.0 37.0 37.0 37.0 8 36.6375 37.0 37.0 37.0 37.0 37.0 9 36.6785 37.0 37.0 37.0 37.0 37.0 10-14 36.5909 37.0 37.0 37.0 37.0 37.0 15-19 36.564 37.0 37.0 37.0 37.0 37.0 20-24 36.4338 37.0 37.0 37.0 37.0 37.0 25-29 36.40220000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.3398 37.0 37.0 37.0 37.0 37.0 35-39 36.3067 37.0 37.0 37.0 37.0 37.0 40-44 36.241200000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.190599999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.161500000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.2116 37.0 37.0 37.0 37.0 37.0 60-64 36.1131 37.0 37.0 37.0 37.0 37.0 65-69 36.0912 37.0 37.0 37.0 37.0 37.0 70-74 36.0342 37.0 37.0 37.0 37.0 37.0 75-79 35.8632371585893 37.0 37.0 37.0 37.0 37.0 80-84 35.92069574708311 37.0 37.0 37.0 37.0 37.0 85-89 35.978091833117986 37.0 37.0 37.0 37.0 37.0 90-94 35.94596245773694 37.0 37.0 37.0 37.0 37.0 95-99 35.86047057259248 37.0 37.0 37.0 37.0 37.0 100-104 35.930215916939304 37.0 37.0 37.0 37.0 37.0 105-109 35.88699280906279 37.0 37.0 37.0 37.0 37.0 110-114 35.83703042074733 37.0 37.0 37.0 37.0 37.0 115-119 35.79888729005978 37.0 37.0 37.0 37.0 37.0 120-124 35.83322382290666 37.0 37.0 37.0 37.0 37.0 125-129 35.71660230282246 37.0 37.0 37.0 37.0 37.0 130-134 35.72735899432969 37.0 37.0 37.0 37.0 37.0 135-139 35.63098524078244 37.0 37.0 37.0 37.0 37.0 140-144 35.592028754197024 37.0 37.0 37.0 37.0 37.0 145-149 35.506037233037475 37.0 37.0 37.0 37.0 37.0 150 35.32076014972646 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 2.0 19 5.0 20 3.0 21 8.0 22 8.0 23 6.0 24 6.0 25 6.0 26 5.0 27 14.0 28 13.0 29 18.0 30 31.0 31 33.0 32 42.0 33 66.0 34 114.0 35 375.0 36 2893.0 37 351.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.625 12.025 12.7 27.650000000000002 2 20.995995995995994 11.036036036036036 38.46346346346346 29.504504504504503 3 18.025 20.849999999999998 31.924999999999997 29.2 4 24.725 26.900000000000002 28.249999999999996 20.125 5 18.725 34.949999999999996 28.125 18.2 6 20.225 39.125 25.374999999999996 15.275 7 14.899999999999999 25.025 44.074999999999996 16.0 8 14.075 28.050000000000004 32.95 24.925 9 18.625 21.85 34.125 25.4 10-14 19.915 29.365000000000002 26.240000000000002 24.48 15-19 20.405 29.035 26.06 24.5 20-24 21.490000000000002 30.125 23.080000000000002 25.305 25-29 21.88 29.34 25.185000000000002 23.595 30-34 21.095 26.064999999999998 24.815 28.025 35-39 21.765 28.865000000000002 24.935 24.435000000000002 40-44 19.48 29.404999999999998 27.21 23.905 45-49 18.205 28.64 29.065 24.09 50-54 21.26 27.994999999999997 26.8 23.945 55-59 19.6 32.1 25.085 23.215 60-64 15.590000000000002 32.47 26.445 25.495 65-69 18.35 29.275000000000002 29.395 22.98 70-74 17.885 31.825 25.555 24.735 75-79 17.847677151572736 32.64989748462269 26.34395159273891 23.158473771065662 80-84 18.732175914344324 30.669935458047732 27.07259718817231 23.525291439435634 85-89 19.439851695976753 30.73300265544366 25.9381732551731 23.888972393406483 90-94 19.18619236365461 29.657317746224475 27.876172796146708 23.28031709397421 95-99 18.47623837063113 29.288408348001006 29.911993965300475 22.323359316067386 100-104 19.20489605988569 28.678367305649687 28.12705477719893 23.989681857265694 105-109 19.008559201141225 31.373547992663543 26.676176890156917 22.941715916038312 110-114 18.624376702822186 30.129028941551432 29.311674291883 21.934920063743384 115-119 20.18533857941603 29.545454545454547 27.45910126320149 22.810105611927938 120-124 18.17518628523787 31.030170392371424 27.778646240425196 23.015997081965505 125-129 20.556552962298024 29.42760587179216 27.99662055127257 22.019220614637234 130-134 21.422455314138926 27.84437546826501 27.865781868778765 22.867387348817296 135-139 20.491936797524026 28.527990443611877 28.305370038551338 22.67470272031275 140-144 21.711146867891028 27.126449536703102 27.703489985019143 23.458913610386727 145-149 21.527107578768096 29.84388305421516 26.806698836219134 21.822310530797616 150 20.932911027929745 30.636337460408868 27.728188885689605 20.702562625971783 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.5 10 1.5 11 1.5 12 1.5 13 1.0 14 0.0 15 1.0 16 2.5 17 2.5 18 2.0 19 3.0 20 3.5 21 3.0 22 3.0 23 3.0 24 6.5 25 10.0 26 11.5 27 14.0 28 18.0 29 20.5 30 39.0 31 53.5 32 62.5 33 94.5 34 131.5 35 110.5 36 59.5 37 94.0 38 161.0 39 143.5 40 141.5 41 195.0 42 215.0 43 256.0 44 363.5 45 347.5 46 234.0 47 220.0 48 213.5 49 163.0 50 119.0 51 73.0 52 28.0 53 13.5 54 11.5 55 11.5 56 8.5 57 5.0 58 4.5 59 2.5 60 1.0 61 2.5 62 3.0 63 8.0 64 28.5 65 38.0 66 29.0 67 13.0 68 1.0 69 4.0 70 30.0 71 46.0 72 46.0 73 44.0 74 19.5 75 2.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 76-77 1.0 78-79 1.0 80-81 0.0 82-83 2.0 84-85 1.0 86-87 5.0 88-89 1.0 90-91 3.0 92-93 1.0 94-95 1.0 96-97 13.0 98-99 12.0 100-101 1.0 102-103 17.0 104-105 16.0 106-107 3.0 108-109 11.0 110-111 24.0 112-113 10.0 114-115 10.0 116-117 9.0 118-119 7.0 120-121 9.0 122-123 23.0 124-125 23.0 126-127 15.0 128-129 24.0 130-131 17.0 132-133 26.0 134-135 16.0 136-137 29.0 138-139 36.0 140-141 33.0 142-143 20.0 144-145 43.0 146-147 35.0 148-149 29.0 150-151 3473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.424999999999997 #Duplication Level Percentage of deduplicated Percentage of total 1 47.812251392203656 15.024999999999999 2 19.72951471758154 12.4 3 11.614956245027845 10.95 4 5.807478122513922 7.3 5 3.1026252983293556 4.875 6 2.6252983293556085 4.95 7 1.352426412092283 2.9749999999999996 8 0.9546539379474941 2.4 9 0.7955449482895784 2.25 >10 6.046141607000796 33.324999999999996 >50 0.15910898965791567 3.55 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGG 88 2.1999999999999997 No Hit GTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCCT 54 1.35 No Hit GGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAG 45 1.125 No Hit CCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATT 39 0.975 No Hit GGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATCC 38 0.95 No Hit GGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCC 37 0.9249999999999999 No Hit GGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTG 34 0.8500000000000001 No Hit GTCGCATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCC 34 0.8500000000000001 No Hit GTAGCCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAA 34 0.8500000000000001 No Hit GCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCATG 31 0.775 No Hit AGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGC 29 0.7250000000000001 No Hit GTTCAGAATCACATCAACAACAAGGTTTTTTTTCCTCCTCTTATGCGATG 28 0.7000000000000001 No Hit GCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCAGA 26 0.65 No Hit CCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACG 26 0.65 No Hit CTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGC 24 0.6 No Hit GGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTA 24 0.6 No Hit GTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCG 24 0.6 No Hit GCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTA 21 0.525 No Hit GTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTCA 20 0.5 No Hit CCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACAT 20 0.5 No Hit GCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATAC 20 0.5 No Hit GCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAAAATGTTCATC 19 0.475 No Hit GCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTACTGGGTCTCAA 19 0.475 No Hit GGAGGAGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCG 19 0.475 No Hit GAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTAC 19 0.475 No Hit GGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACAT 19 0.475 No Hit GCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGA 19 0.475 No Hit GTGGCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTG 18 0.44999999999999996 No Hit CTTCATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTTTAACAAA 18 0.44999999999999996 No Hit CCGGGGTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTC 18 0.44999999999999996 No Hit CTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCAT 18 0.44999999999999996 No Hit GCCGTGGAGGAGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGT 17 0.42500000000000004 No Hit GCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTGGCGC 17 0.42500000000000004 No Hit CTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCCAA 17 0.42500000000000004 No Hit GCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAAT 17 0.42500000000000004 No Hit CTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTT 17 0.42500000000000004 No Hit GGAGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCG 16 0.4 No Hit GGTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCA 16 0.4 No Hit CCTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGA 16 0.4 No Hit GCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCCT 16 0.4 No Hit GTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAG 15 0.375 No Hit GTGGAGGAGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGC 15 0.375 No Hit ACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAG 15 0.375 No Hit GCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGAG 14 0.35000000000000003 No Hit ATACAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCC 14 0.35000000000000003 No Hit GGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGCTC 14 0.35000000000000003 No Hit GTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGGC 14 0.35000000000000003 No Hit GGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGC 13 0.325 No Hit GGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGC 13 0.325 No Hit CCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCC 13 0.325 No Hit AAGCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTAC 12 0.3 No Hit TTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAATTG 12 0.3 No Hit GCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAA 12 0.3 No Hit CCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAA 12 0.3 No Hit GCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTG 12 0.3 No Hit CTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTT 12 0.3 No Hit GGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCAGG 11 0.27499999999999997 No Hit GCAAAAGCATGTTAAATAGCAAGGCACATTGCCAAAAAAAAATAGAACAT 11 0.27499999999999997 No Hit CAATTTTCATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCCTGA 11 0.27499999999999997 No Hit ATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACA 11 0.27499999999999997 No Hit GTCCAAGCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGG 11 0.27499999999999997 No Hit ATATTCTTCATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTTTA 11 0.27499999999999997 No Hit GTATTTTTCTGAGGATCTTATCTTCAACAGAAGCCAGAGCTTCAAATTGC 11 0.27499999999999997 No Hit GCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCA 11 0.27499999999999997 No Hit GCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTT 11 0.27499999999999997 No Hit CCCATATAAGCCTCTCGGGTGCCCACGATCCAAACCAACTCCCTTCAATT 11 0.27499999999999997 No Hit CTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTT 11 0.27499999999999997 No Hit AGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAA 11 0.27499999999999997 No Hit ATTCAATATTGCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGA 10 0.25 No Hit ATGGAAGACCACCCATCCACAAGCAAAACCATCCATTCCTGACTCCTGAT 10 0.25 No Hit CACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACAC 10 0.25 No Hit TATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCCTCAAATAATCAT 10 0.25 No Hit CAAACGTATTGGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTG 10 0.25 No Hit GCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATA 10 0.25 No Hit CAAGCGAGCTCCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTA 10 0.25 No Hit CTTAAGTTTTGAAAAGTTAGGTGGTAGCTCTCCACTTAAACCACAACTAT 10 0.25 No Hit GCTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTT 10 0.25 No Hit GTTGTAAAGCGCTTCATCTAACTTTGCAATTTGTTCGAACAGAGGTGCAA 10 0.25 No Hit GCCGACTCGGCGAACTCGCCGTGGAGGAGCACGCAGAGGAGAACCAGCGC 9 0.22499999999999998 No Hit CCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTT 9 0.22499999999999998 No Hit CTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTC 9 0.22499999999999998 No Hit AGGCAAACTTCAATAGATATAAGAAGTTAACTACCGGGGTACACAGTTCA 9 0.22499999999999998 No Hit CATGTAGTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCC 9 0.22499999999999998 No Hit CTCATGGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCA 9 0.22499999999999998 No Hit AATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCA 9 0.22499999999999998 No Hit GTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCC 9 0.22499999999999998 No Hit AAGGGAATCAGGCTCATGGATAGACTTCATAGCTTCATCAATAGCTGATT 9 0.22499999999999998 No Hit CCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGG 9 0.22499999999999998 No Hit CTAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGAT 8 0.2 No Hit GTCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 8 0.2 No Hit GTCATTTGCAACTCACACACACACAAAACTCAATTGCGCATCAAGAATTT 8 0.2 No Hit GCACTAGCTGATGCTCGAGGAACCTGAGTTGCTCTTATTGAGTATACGTC 8 0.2 No Hit GTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTCAGA 8 0.2 No Hit GTAGTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTAT 8 0.2 No Hit GTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCA 8 0.2 No Hit CGACAATCTTTTTCTGACAACTTGTGTACTCGCCGAGCAGTGCCTGGTAT 8 0.2 No Hit CTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCTTGAACACTGCGTCTC 8 0.2 No Hit GACTCGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCC 8 0.2 No Hit CCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTG 8 0.2 No Hit TGGAGGAGCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCC 8 0.2 No Hit CACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCT 7 0.17500000000000002 No Hit CCCTTCAATTGCTCCAAGATTTCGTTTGTACGCAACATCAAACAGCTTTT 7 0.17500000000000002 No Hit GTTCTGTAGTTCAGGAAACAAAGTTCTCAGGTTCTTCTTCTCTGCTTGAC 7 0.17500000000000002 No Hit CGGCGAACTCGCCGTGGAGGAGCACGCAGAGGAGAACCAGCGCCAGCACG 7 0.17500000000000002 No Hit CCCCAGAGCAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTGGCGCGAT 7 0.17500000000000002 No Hit ATTTTAACAAAGTATAGTATCCCCTATTTTTTCCTCAAATAATCATCTCG 7 0.17500000000000002 No Hit GGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTATCCGGTGAT 7 0.17500000000000002 No Hit ACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACG 7 0.17500000000000002 No Hit GGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCTTTCAG 7 0.17500000000000002 No Hit GTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGCAAGGGAATCA 7 0.17500000000000002 No Hit CATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAA 7 0.17500000000000002 No Hit CGAGTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGA 7 0.17500000000000002 No Hit GTCTTGGATATGGAGGAACTGTTAATCATGATTAGATTTTTTTTCAAAAA 7 0.17500000000000002 No Hit CCCTGATGTGTTGAGTCAACAACTCCTCCTTGAGCCACCTCATCTAACAG 7 0.17500000000000002 No Hit GCACGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCA 7 0.17500000000000002 No Hit CTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATT 7 0.17500000000000002 No Hit GCAGGATACTTGCTATTTTTGCCAACTTCTTATCCAGATACAGGCAGTTC 7 0.17500000000000002 No Hit GTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTT 6 0.15 No Hit CAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACTTTGCAAT 6 0.15 No Hit CTGCAGAACTGACTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCA 6 0.15 No Hit CCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATA 6 0.15 No Hit CGCAACATCAAACAGCTTTTTTTTCATGCCGCCCTTCTCAGCAACCTTCT 6 0.15 No Hit CAAGATTTCGTTTGTACGCAACATCAAACAGCTTTTTTTTCATGCCGCCC 6 0.15 No Hit CACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACATAA 6 0.15 No Hit ATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTCCTCAAATAATCA 6 0.15 No Hit TGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTTTTTC 6 0.15 No Hit CCAGTTAATTGTGGTGGTGCTGCATCAAGTATTTCACGAGATAGCTTTGC 6 0.15 No Hit TTTTTTTTTTGATAATGCAAACATATAAAGGTTCCAATGTCAAGATATTA 6 0.15 No Hit CCAAGATTTCGTTTATACGAAACATCAAACAGCTTTTTTTTCATGCCGCC 6 0.15 No Hit ATCCCATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAA 6 0.15 No Hit CATAAACTGTGCTTCCAATAAGATTTTTTGAACCTTGTGCGATACCATTT 6 0.15 No Hit CCTTGTTCTTGACGCAGCCCAATCTTTATTAATGAGGAGAACTAAACATG 6 0.15 No Hit ACGAGATAGCTTTGCAAGGGAATCAGGCTCATGGATAGACTTCATAGCTT 6 0.15 No Hit CCTATTTTTTCCTCAAATAATCATCTCGCTGAGCTGGATTGAGTCCTAAA 6 0.15 No Hit CAGGGGCCGGCCAGCAGATGGTGGACGGGGTACTGGCGCGATGACAACTA 6 0.15 No Hit ATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTTTAACAAAGTAT 6 0.15 No Hit CTAAAGCTAATATGCGTGCATGAGCACACATATTTCTCCGATAATCAACA 6 0.15 No Hit GCACTACTGACTGCATAAACTGTGCTTCCAATAAGATTTTTTGAACCTTG 6 0.15 No Hit TAAACCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATC 6 0.15 No Hit GGCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGAA 6 0.15 No Hit CTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAACT 6 0.15 No Hit GTGGAGTTGAGTATTCAATTGGGTTACACCAATGGCCATCGGCATAGAAA 6 0.15 No Hit GCACTTCTGACTCTTTATCCTGTATTTTTCTGAGGATCTTATCTTCAACA 6 0.15 No Hit GGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATGTGTTGAGTCAACA 6 0.15 No Hit CCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGAT 6 0.15 No Hit CCACGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTTCGTTTATACG 6 0.15 No Hit ATGTAGTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCT 6 0.15 No Hit GGATAGACTTCATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGA 6 0.15 No Hit CTTTGGATGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACATTAT 6 0.15 No Hit GTACAATTTTTTTCTCCCACTCAAAGCTCGACCTCAAACCTCCACATCAA 6 0.15 No Hit GGCCATGTTATTGAATCTTCAATAGCATTTTTTATAGTGTCCTCGAGAGC 5 0.125 No Hit CCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTAAC 5 0.125 No Hit TTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAATTGTAC 5 0.125 No Hit GTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGGTGAAAACATCCAT 5 0.125 No Hit GGGTGAAAACATCCATCAGCAGGGCCGGGCCTCCTGCCTGTAAGTACATC 5 0.125 No Hit CATATAAGCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGC 5 0.125 No Hit CTCTTATTGAGTATACGTCCGGGTGATATTCTTCATCAAAAAGGCCTTGA 5 0.125 No Hit GCCTCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGA 5 0.125 No Hit GTTCTTTGGATGGCAAACTACGTTATCAATAACAGTGCCGGGAGGGACAT 5 0.125 No Hit ATTCGGGAAAAAGTGGAGGGGTGAAAACATCCATCAGCAGGGCCGGGCCT 5 0.125 No Hit GGGGTACACAGTTCAGAAGTACAGAAACAAGCTAGGCAAAAAGACTTCCA 5 0.125 No Hit GCAAAATCAGAGCCCGCATTGACACAAGAGGTGAAGTATTTTTGTTCCCC 5 0.125 No Hit GGCTTGAAGATAATGCTATCCCATATAAGCCTCTCTGGTGCCCATGATCC 5 0.125 No Hit GGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTCATCTA 5 0.125 No Hit AGTGACTTCAATGATTATTTCTATCCTAGTTGTGGAGTTGAGTATTCAAT 5 0.125 No Hit CCGTATCCTCCCAGCTGCTCCTTCTGGAATTGCAAAGGGGTCCTCAGTTA 5 0.125 No Hit GTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTGTAAAGCGCTTC 5 0.125 No Hit CTCTATTGACAATGATACACATGATTTTTTCGCTGATGTCCTGAAAGAGG 5 0.125 No Hit CTGGAATCTCTTTGTGAATTGAGACAACAGGGAATTCATCTTGTTCCATT 5 0.125 No Hit GTCAAAGTTTCAGAAGTTTGACTTAGGACAAAGTTAGGACTTGTTACATT 5 0.125 No Hit CTCTAAAGCTAATATGCGTGCATGAGCACACATATTTCTCCGATAATCAA 5 0.125 No Hit CGGCTTTTAATTGCAAGGTCTCTGCAGAACTGACTGGAATCTCTTTGTGA 5 0.125 No Hit TTTTTTTTTGAGACAGTATAGACAAATTGAGGCCTCCTTAAATACACGCC 5 0.125 No Hit CTCAGGTTTCCCCACAATCCATAGTCATACCTCGCGAAAGTGCCTTTCCT 5 0.125 No Hit ATAGCTTCATCAATAGCTGATTTCAAATGAGCCAGTGGATCTTTTTCAAT 5 0.125 No Hit AGTCTGGCAAATTCTGATATTATTTTAACAAAGTATAGTATCCCCTATTT 5 0.125 No Hit GCGATGCTCAGGCAGACAAGGCAGGGCTCTGCAGTTCCATAACCATAAAA 5 0.125 No Hit GAGGAATAGTATCGGGAACTGATGAGAGTTCCACTTTAAAATGGCTAGGA 5 0.125 No Hit TCTCTGGTGCCCATGATCCAAACCAACTCCCTTCAATTGCTCCAAGATTT 5 0.125 No Hit TTTTTTTTTAAACATCATAACAGGTTCTCCATTTAGGTAAATAGCATTTC 5 0.125 No Hit GACACATCTGGTGGGCATAGAGCGCAGAGAATAAACAGAAGGCCCTGATG 5 0.125 No Hit CCCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGATACACA 5 0.125 No Hit CCAGCTCTTCCAACATTGACAATCACTGGCTTCACAAGGGCGCTCTTTGC 5 0.125 No Hit CCGCCACCCACACCTCATAAGGGCCGCACCCTTCAAAAAAAGGATCTGAT 5 0.125 No Hit GCCAAACAAATAGGTCACTGCAAGTCCTTATTCGGGAAAAAGTGGAGGGG 5 0.125 No Hit GTTAGGGTTTGTACAGCGCCATTCGCGGCGCTTTTTCTGCTCCATGATCT 5 0.125 No Hit CTTGCCGGTCAGTTTGGGCCTCAAGCCAGTGCTGCTCGAACTGAATGTTG 5 0.125 No Hit CGCAGAGGAGAACCAGCGCCAGCACGGCCGCGCCGTTGCCGCCGGCACGA 5 0.125 No Hit CCATATTCTTCATTTTAGATCATGTAGTCTGGCAAATTCTGATATTATTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR5262813 read2 length is 62-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262813_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 62-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.097 37.0 37.0 37.0 37.0 37.0 2 36.0855 37.0 37.0 37.0 37.0 37.0 3 36.06 37.0 37.0 37.0 37.0 37.0 4 36.123 37.0 37.0 37.0 37.0 37.0 5 36.1735 37.0 37.0 37.0 37.0 37.0 6 36.0985 37.0 37.0 37.0 37.0 37.0 7 35.9985 37.0 37.0 37.0 37.0 37.0 8 36.217 37.0 37.0 37.0 37.0 37.0 9 36.1555 37.0 37.0 37.0 37.0 37.0 10-14 36.218199999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.1821 37.0 37.0 37.0 37.0 37.0 20-24 36.072900000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.0756 37.0 37.0 37.0 37.0 37.0 30-34 36.025099999999995 37.0 37.0 37.0 37.0 37.0 35-39 35.9844 37.0 37.0 37.0 37.0 37.0 40-44 36.005900000000004 37.0 37.0 37.0 37.0 37.0 45-49 35.9386 37.0 37.0 37.0 37.0 37.0 50-54 35.9325 37.0 37.0 37.0 37.0 37.0 55-59 35.8832 37.0 37.0 37.0 37.0 37.0 60-64 35.8591871467867 37.0 37.0 37.0 37.0 37.0 65-69 35.904426106526635 37.0 37.0 37.0 37.0 37.0 70-74 35.782195548887216 37.0 37.0 37.0 37.0 37.0 75-79 35.79691020218973 37.0 37.0 37.0 37.0 37.0 80-84 35.73418119144914 37.0 37.0 37.0 37.0 37.0 85-89 35.70255992732245 37.0 37.0 37.0 37.0 37.0 90-94 35.64421518172368 37.0 37.0 37.0 37.0 37.0 95-99 35.67124035169324 37.0 37.0 37.0 37.0 37.0 100-104 35.54709643802022 37.0 37.0 37.0 37.0 37.0 105-109 35.53682778181196 37.0 37.0 37.0 37.0 37.0 110-114 35.55885883786673 37.0 37.0 37.0 37.0 37.0 115-119 35.498216933658206 37.0 37.0 37.0 37.0 37.0 120-124 35.480077520793344 37.0 37.0 37.0 37.0 37.0 125-129 35.364136619275044 37.0 37.0 37.0 34.6 37.0 130-134 35.38083595669006 37.0 37.0 37.0 34.6 37.0 135-139 35.35631796982489 37.0 37.0 37.0 32.2 37.0 140-144 35.33065194559477 37.0 37.0 37.0 32.2 37.0 145-149 35.25979298533384 37.0 37.0 37.0 32.2 37.0 150 35.201272411798726 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 5.0 13 3.0 14 2.0 15 1.0 16 2.0 17 0.0 18 0.0 19 1.0 20 7.0 21 5.0 22 8.0 23 10.0 24 7.0 25 8.0 26 9.0 27 14.0 28 14.0 29 14.0 30 24.0 31 46.0 32 61.0 33 98.0 34 224.0 35 591.0 36 2596.0 37 248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.625 20.4 8.75 23.225 2 26.924999999999997 21.75 28.775000000000002 22.55 3 22.375 25.624999999999996 31.874999999999996 20.125 4 25.8 29.825000000000003 22.3 22.075 5 23.275000000000002 35.099999999999994 19.725 21.9 6 23.724999999999998 34.55 23.150000000000002 18.575 7 21.8 19.075 35.675000000000004 23.45 8 20.9 22.8 26.025 30.275000000000002 9 19.475 25.650000000000002 28.125 26.75 10-14 24.065 26.105 26.07 23.76 15-19 23.86 26.174999999999997 27.189999999999998 22.775000000000002 20-24 23.57 25.75 27.66 23.02 25-29 23.189999999999998 26.419999999999998 27.755000000000003 22.634999999999998 30-34 23.695 26.979999999999997 27.400000000000002 21.925 35-39 23.18 27.705000000000002 26.685 22.43 40-44 24.68 26.02 26.87 22.43 45-49 23.87 25.759999999999998 27.965 22.405 50-54 23.169999999999998 26.495 28.005000000000003 22.33 55-59 23.36 27.57 26.99 22.08 60-64 23.387338733873385 26.267626762676265 28.797879787978797 21.547154715471546 65-69 25.061265316329084 26.431607901975497 27.106776694173547 21.40035008752188 70-74 24.996249062265566 26.296574143535885 27.021755438859714 21.685421355338836 75-79 23.95958383353341 25.51520608243297 28.671468587434973 21.853741496598637 80-84 25.870696557245797 25.24019215372298 27.942353883106485 20.94675740592474 85-89 24.97119094142993 27.03041234530788 27.1256074953655 20.87278921789669 90-94 26.08499322663188 26.958005117655915 26.56665495961066 20.39034669610155 95-99 26.15036459642947 25.71284888106613 27.08574302237868 21.051043500125726 100-104 25.749835617824086 27.105356329978253 26.144352840018207 21.000455212179457 105-109 25.738740574689217 27.017525983289183 26.553902588139394 20.68983085388221 110-114 24.866337651655357 26.557680444170266 27.51902118034135 21.056960723833022 115-119 25.208430428253326 25.845373103412562 27.228004764124076 21.718191704210035 120-124 25.377879703950796 26.623579693526526 26.696549567392893 21.301991035129785 125-129 25.6536206623356 26.87371256536207 26.092008662124332 21.380658110177997 130-134 25.352112676056336 26.342847962298503 27.46211106945858 20.84292829218658 135-139 24.669531632486535 26.883533699613775 26.562584997008106 21.884349670891584 140-144 24.26671119274225 25.58023042244114 28.368675905827352 21.784382478989258 145-149 24.774877464949277 26.410577909495043 28.222956799270488 20.591587826285192 150 24.03123192596877 26.865240023134763 28.484673221515326 20.618854829381146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 0.5 20 0.5 21 2.0 22 1.5 23 1.0 24 2.5 25 2.0 26 1.0 27 1.5 28 5.5 29 5.0 30 7.0 31 13.5 32 17.5 33 23.5 34 26.5 35 39.5 36 71.5 37 88.0 38 109.5 39 148.0 40 164.5 41 175.5 42 208.0 43 235.0 44 286.0 45 289.5 46 239.5 47 203.0 48 186.5 49 183.0 50 162.5 51 184.0 52 205.0 53 153.0 54 79.0 55 55.0 56 41.0 57 33.0 58 34.5 59 24.0 60 17.5 61 15.0 62 20.5 63 22.0 64 18.5 65 19.0 66 11.5 67 11.0 68 16.5 69 24.5 70 28.5 71 21.0 72 14.5 73 14.5 74 14.0 75 6.0 76 2.5 77 4.0 78 4.0 79 3.0 80 1.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 62-63 1.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.0 78-79 1.0 80-81 0.0 82-83 1.0 84-85 1.0 86-87 5.0 88-89 1.0 90-91 3.0 92-93 1.0 94-95 1.0 96-97 13.0 98-99 12.0 100-101 1.0 102-103 17.0 104-105 16.0 106-107 3.0 108-109 11.0 110-111 24.0 112-113 11.0 114-115 10.0 116-117 9.0 118-119 7.0 120-121 9.0 122-123 23.0 124-125 23.0 126-127 15.0 128-129 25.0 130-131 17.0 132-133 28.0 134-135 18.0 136-137 30.0 138-139 39.0 140-141 34.0 142-143 21.0 144-145 45.0 146-147 35.0 148-149 30.0 150-151 3458.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.9 #Duplication Level Percentage of deduplicated Percentage of total 1 56.1122244488978 28.000000000000004 2 19.789579158316634 19.75 3 11.723446893787576 17.549999999999997 4 5.761523046092185 11.5 5 2.7054108216432864 6.75 6 1.503006012024048 4.5 7 0.6513026052104208 2.275 8 0.6513026052104208 2.6 9 0.30060120240480964 1.35 >10 0.8016032064128256 5.7250000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCC 34 0.8500000000000001 No Hit GTTGAACTAAAGGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAA 20 0.5 No Hit GTCTGATTTAAAAAGCTACCATACCTGTTCCGTTGTGGTCAAATCTTATG 18 0.44999999999999996 No Hit CTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTTCTCTT 15 0.375 No Hit GTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAGCAGCAGA 15 0.375 No Hit ACTTAACCAACTGAAACACTTGACAACATTATCTTTCCATGCGGTTGAGG 15 0.375 No Hit GAAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAA 14 0.35000000000000003 No Hit CAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCT 13 0.325 No Hit CTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTT 12 0.3 No Hit CTCGCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCA 11 0.27499999999999997 No Hit CCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTT 11 0.27499999999999997 No Hit GGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTTAAAAC 11 0.27499999999999997 No Hit GTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGAATCAA 10 0.25 No Hit CCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTT 10 0.25 No Hit GGGAGATGTTTATCTGGCATACCTTCCATTGGCTCATGTTTTTGAACTAG 10 0.25 No Hit GCGACAGGCCAAGAAGAAGGCTCCCAAGCCAAAGACAAACTAGAGCACCC 10 0.25 No Hit GGCTTATTCATGCTTGAATTTGAAGAGCATAGTGAAACAAATGTTCCGAG 9 0.22499999999999998 No Hit GGCTGACGGCCGCTGCGGGCATGGGGTGGAGGCAGCCGAGGGAGCAGGAG 9 0.22499999999999998 No Hit GTCAAGTCCGCGATTCTCGTGACAAGACTAGCGATAGATTTGACTTCATT 9 0.22499999999999998 No Hit GTTGTGTTTGCACAGTGAGCCAGTGAGGTAGCCTGGTGCCCCGCGCTAAA 9 0.22499999999999998 No Hit GACAAACTAGAGCACCCAAGGTTATTGAAGTGTTGCGGGGGAGACGGTCT 9 0.22499999999999998 No Hit GCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGC 9 0.22499999999999998 No Hit CGCACTCTTCGGCGCGCTTCTTTTCTTCCTGGGCATGAAGAACTCCATTC 8 0.2 No Hit GTATTTCTCACAGGTCACACAGTGCGCGCAGCAGCAACACGACCAATGGA 8 0.2 No Hit GCTTCTTTTCTTCCTGGGCATGAAGAACTCCATTCCCAAGCGACAGGCCA 8 0.2 No Hit GGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAAT 8 0.2 No Hit GATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAA 8 0.2 No Hit GATTACGCATGGCAACATGGTGGCCACAACTGCTGCAGTCAGGACAATCA 8 0.2 No Hit AGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACC 8 0.2 No Hit GCTGAATCCTCTGAGGATTAATGTTCAGTTAAAGAACAGCCATGCTGGTG 8 0.2 No Hit CTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTTC 8 0.2 No Hit ATTCACTGATCATAGATCTGGTTCATCTGGTGGAAGGTCAGCTGGCTATG 8 0.2 No Hit AATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGT 8 0.2 No Hit GTGAAATTGGAATCGTGTTTAGGACTCAATCCAGCTCAGCGAGATGATTA 8 0.2 No Hit CTTCATTCTGACTTACACTGATGAGGACGGGGATGTTGTCATGCTGGATG 8 0.2 No Hit TGGATGGCAAGTACGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGT 7 0.17500000000000002 No Hit GAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTT 7 0.17500000000000002 No Hit GGACCTTCCATGTGAAGTAATAGGCTAATACGGAGTAGCTTCCAGGAAAT 7 0.17500000000000002 No Hit GAGAAGGGCCATTCTTTCAGCAGAGAAATAAAGAAATCTTTGAGACAGAA 7 0.17500000000000002 No Hit AGAAGATGAAGCATACTGCATAATTCCTGTTGAAATCTTGCATGTATATC 7 0.17500000000000002 No Hit GCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGTGGTCA 7 0.17500000000000002 No Hit AGTGTTCACAGGTGCACCTGGAAAGTATGTTGAACTAAAGGAGAGTGTTC 7 0.17500000000000002 No Hit GGTGTCTCTGTCGTGGATAGATTTACTTTCTATACACTTGCGTTCTTTGA 7 0.17500000000000002 No Hit GGGACGTGCTGCTGCACTGCGTGGTGGACGCGATTCTTGGCGCCCTGGGG 7 0.17500000000000002 No Hit GGTTATTGAAGTGTTGCGGGGGAGACGGTCTGATTTAAAAAGCTACCATA 7 0.17500000000000002 No Hit CTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAA 7 0.17500000000000002 No Hit CATTCCTAAACTTGGCACGGGAGACGTTTATCTGGCATACCTTCCATTGG 7 0.17500000000000002 No Hit ATTTCAGTTCCTGCAATTTTGGATCGCATAAGAGACGCCGTGTTCAAGAA 7 0.17500000000000002 No Hit CTGCATAATTCCTGTTGAAATCTTGCATGTATATCAGACATTTTTTAGAA 6 0.15 No Hit GCAGCGGCCACCCTTATGGTCAGCTCCATGCCCGCAACACGTACACTGGA 6 0.15 No Hit GTCAAGCCTTGGACATGCTCACAGCCACAGGGTTGGATCTGCCAGGGAAC 6 0.15 No Hit AGAACGGCGTGCCTCGTGGTCCTCTGTGTGCTGCCCTTCTTGCGCCCGGC 6 0.15 No Hit AGGAGATTGAGAAGGGCCATTCTTTCAGCAGAGAAATAAAGAAATCTTTG 6 0.15 No Hit GTCTAGAGAGCTTGGATAACCGGATGTCAAGCGAGGTGACCAGCTGTGCA 6 0.15 No Hit GTTAGATCAAATAAATGAGGCATGCAAATTCTTGGATGAAAGTTGGTCCC 6 0.15 No Hit AGGAGCACTACAACACTGCTCGTGGTGTCCAGAAGGTTCTCCAGAATTAC 6 0.15 No Hit AAAAGATCTGTGAAATTGGAATCGTGTTTAGGACTCAATCCAGCTCAGCG 6 0.15 No Hit GGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCAG 6 0.15 No Hit CGATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGG 6 0.15 No Hit GTACAGGTCTGCCCAAGGGTGTAATGATTACGCATGGCAACATGGTGGCC 6 0.15 No Hit CTTTAAAACCCACACCAAACCCTTTGAGACCCAGTACAATTGAAAAAGAT 6 0.15 No Hit GTTAACAGTTGCTCGCGCTAGGAAAATTCAGCGGTTCCTTAGCCAGCCTT 6 0.15 No Hit AGCAAATAGTCCAAATCCACGCGCGGCCTGGAATAATGTTTGTCTTTCTC 6 0.15 No Hit GACGTTTCTGTACTGAAACCCACTCTTATGATTTCAGTTCCTGCAATTTT 6 0.15 No Hit GATGATCTTTCTGAGCAGTCATTTTACATGGTTGGTGGAATAGATGAGGT 6 0.15 No Hit GGCTCAGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGAC 6 0.15 No Hit GTTCAGTTAAAGAACAGCCATGCTGGTGCATCTCAGGTCATCCAGCAGGA 6 0.15 No Hit GCGAGGATGGGGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTC 6 0.15 No Hit CCGGCTGCGGGTATCCGCTTCGTGATCGATAGGGAGGAGTGCTTCTCGCA 6 0.15 No Hit GGTAGCCTGGTGCCCCGCGCTAAACTAAAAAGATCTGTGAAATTGGAATC 6 0.15 No Hit AGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTT 6 0.15 No Hit CTTCAAGCAGCTTACAGAGGCCAGCCAGCATTTCACCTCCGCGGTCACAG 6 0.15 No Hit GGACAATCATTCCTAAACTTGGCACGGGAGATGTTTATCTGGCATACCTT 6 0.15 No Hit CGCTAGCAGCCTCCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAA 6 0.15 No Hit GACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTTTT 6 0.15 No Hit GCACAAGGAGTTGTGTTTGCACAGTGAGCCAGTGAGGTAGCCTGGTGCCC 6 0.15 No Hit AGGTCATCCAGCAGGAATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAG 6 0.15 No Hit GCTAATTTCAGTTACATGGGAATCGGAAGAGCTAAAAGAAGGGTAGAAAC 6 0.15 No Hit GCTAGCTATTCCCCCACTGCACCGGGCTACTCCCCTTCGTCCACTGGACC 5 0.125 No Hit AAATCCCCATATCACGAAACCATAGACTTTGACGTGCATGTTGGTCATTT 5 0.125 No Hit CATGCACTCTTCAAAGATCCAACCCCGGCTCGACAAGTTCAGACTTCGAC 5 0.125 No Hit GTTCTCTCTCAGGGACGGTGACAAGCCACACGAGATGACTTTCTAAGTTG 5 0.125 No Hit GCTTGCTTTTGCACTTGCAAACACCCTTTTTTCTCTTCCTGAACCAAAGG 5 0.125 No Hit ATCAAGTCCTACGCCGCTTAGGGCCATCGTTCAGGAAGATCCATTTGCTC 5 0.125 No Hit CCTCCGAGAACTCAAGTTCAGCCTTTAACCGAGAGTTATTGGGAAGAGAA 5 0.125 No Hit GTTGGATCTGCCAGGGAACCATGCACTCTTCAAAGATCCAACCCCGGCTC 5 0.125 No Hit GGACAGAGTCGGATCCTTCTCCAGTGAAGGCAGTCGGTGTGAAAAGTGAT 5 0.125 No Hit GAGCAGCGTGAGATGGTTAAACAATCGACACTGCAGAACATCTCTGATAT 5 0.125 No Hit GTGACAAGCCACACGAGATGACTTTCTAAGTTGGTACAAGTTTCTGAGTT 5 0.125 No Hit GGCTATGTCAATGGAACACATTTCACTCTGAATTTATCTGCTCTTCGGTC 5 0.125 No Hit TGTCTCCAGAGATTATTGAATTATCCGACGAATGTTGATGTGCAGAAGCT 5 0.125 No Hit GTTAGCTTCTGGTGTTGCTATTGGATATGGCTCAGCTCTGACTATGACTG 5 0.125 No Hit TTACATGGTTGGTGGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCG 5 0.125 No Hit AGAGGTTCAACGGCTATGTCAATGGAACACATTTCACTCTGAATTTATCT 5 0.125 No Hit ATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGCGTAGAAA 5 0.125 No Hit GTAGAAATCTCCTCACATTTTAACTTTTTGTGGTCAACCTGTTGTGCCTT 5 0.125 No Hit CTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAAAGACAA 5 0.125 No Hit GCTTCACAGGAGTTTTCCGCTTGATTTTCAAACCGCTGGTCGAAGAACTA 5 0.125 No Hit CGTGACAAGACTAGCGATAGATTTGACTTCATTGTTCAGAAGAGAGGGGT 5 0.125 No Hit AGGGATCCCATTTCTTGTTGAATTTAACTGATGTTATGTTTGAGAGATTT 5 0.125 No Hit GGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTCGTG 5 0.125 No Hit GGTTCACCGCTTCTGCTTCACCAATAAATCCCCATATCACGAAACCATAG 5 0.125 No Hit ACGAGGGGGACACTGTCCATGTATCCTTCGTGGTGATCAAGGCCGACACG 5 0.125 No Hit GGAATAGATGAGGTCATTGCCAAGGCAGAGAAGATCGCCAAGGAGAATGC 5 0.125 No Hit AGAGAAGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTT 5 0.125 No Hit ATTGACTGAGGCATCTTGTCCCACTTGTGCATAGTGTTCTTTTTAACCAT 5 0.125 No Hit GTTCAGGAAGATCCATTTGCTCAGATAAAATCAGTTATTGATGAAGCTTT 5 0.125 No Hit GGAGAGTGTTCAAAGTTTCCAGGGTGTATTGGATGGCAAGTACGATGATC 5 0.125 No Hit ATTGTGTAGGAGTACCCTATGAGTCGGTATCACCAGATCCGAACTCACTA 5 0.125 No Hit AGTGAGGTAGCCTGGTGCCCCGCGCTAAACTAAAAAGATCTGTGAAATTG 5 0.125 No Hit GTTGAAGCATGTGATTGCTGCTACCATTGGTTTGAAGGGTCTGGGAGGTC 5 0.125 No Hit AGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAAAG 5 0.125 No Hit GCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACAAAAGG 5 0.125 No Hit CCATCCTTCATTCCTTTGCTTGCTTTTGCACTTGCAAACACCCTTTTTTT 5 0.125 No Hit GTTTTTGAACTAGCAGCAGAGACTGTCATGTTAGCTTCTGGTGTTGCTAT 5 0.125 No Hit GAGTTGTGTTTGCACAGTGAGCCAGTGAGGTAGCCTGGTGCCCCGCGCTA 5 0.125 No Hit AGGGGACAAAAGGAGATGTTTCTGTACTGAAACCTACTCTTATGATTTCA 5 0.125 No Hit GATTGAAAACCGTATCCTCTATGCTGCACGTGGAATCTTCAACAAATTTA 5 0.125 No Hit GAACCAACAAGTTTGAGGGGAATCCTTCATTCCTTTGCTCTTGGATTTGT 5 0.125 No Hit GGCACAAGGAGTTGTGTTTGCACAGTGAGCCAGTGAGGTAGCCTGGTGCC 5 0.125 No Hit GCTCGGCTCTGACTATGACTGATACATCAAATAAGATAAAGAAGGGGACA 5 0.125 No Hit AGATCGCCAAGGAGAATGCGTAGAAATCTCCTCACATTTTAACTTTTTGT 5 0.125 No Hit AAGAATTCAGGGAATTTCTTAAAGCTCAGGGTAATCAGAACAACAATGGC 5 0.125 No Hit GTCCTTGAAGGTAGAAAATGTTGTGGACTTCAGTTGGTCACCCACTGATC 5 0.125 No Hit CTGGAATTGAATCTTTCACATTTGATGAAGTGTATAGCAATGGGCTGGGT 5 0.125 No Hit GGTGGATCTTGTTGTTAAAGATCCTAATGGAGGTCAAGTCCGCGATTCTC 5 0.125 No Hit GGAAAATTGTTGCTTCAATGCATCACGGATTGACTATTACTTGGAGTATG 5 0.125 No Hit AAGAACTCCATTCCCAAGCGACAGGCCAAGAAGAAGGCTCCCAAGCCAAA 5 0.125 No Hit CTGGAACGGGGATGATGTAGAGATGCTTGGTTTTTTGCATTACATTTTTT 5 0.125 No Hit GCTTTTAAGTTCGCCCTGGATGCTGACTTCATTCTGACTTACACTGATGA 5 0.125 No Hit GGCATCAAGTGCGACTGCTCCTACAACGTCAGCACCGTCTGCCACATCAC 5 0.125 No Hit CTTTGACGTGCATGTTGGTCATTTTTCATATTTCGAACAACATGCCAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881600 spots for ERR5262813.sra Written 1881600 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra Read 1881590 spots for ERR5262813.sra Written 1881590 spots for ERR5262813.sra SRR ids: ['ERR5262813.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wd77uc7j ERR5262813.sra spots: 37631810 blocks: [[1, 1881590], [1881591, 3763180], [3763181, 5644770], [5644771, 7526360], [7526361, 9407950], [9407951, 11289540], [11289541, 13171130], [13171131, 15052720], [15052721, 16934310], [16934311, 18815900], [18815901, 20697490], [20697491, 22579080], [22579081, 24460670], [24460671, 26342260], [26342261, 28223850], [28223851, 30105440], [30105441, 31987030], [31987031, 33868620], [33868621, 35750210], [35750211, 37631810]] ERR5262813 file size 12380043 ERR5262813 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262813 ERR5262813_1.fastq ERR5262813_2.fastq Input file: ERR5262813_1.fastq Paired file: ERR5262813_2.fastq trimmed: ERR5262813-trimmed-pair1.fastq, ERR5262813-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:31:16 2024 >> started Fri Dec 6 12:32:00 2024 >> done (43.729s) 37631810 read pairs processed; of these: 1 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 37631809 (100.00%) read pairs available; of these: 11100 ( 0.03%) trimmed read pairs available after processing 37620709 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 2 0.00% 23 1 0.00% 24 1 0.00% 25 1 0.00% 26 1 0.00% 27 1 0.00% 28 2 0.00% 29 2 0.00% 30 3 0.00% 31 2 0.00% 32 3 0.00% 33 8 0.00% 34 3 0.00% 35 0 0.00% 36 2 0.00% 37 3 0.00% 38 3 0.00% 39 6 0.00% 40 5 0.00% 41 0 0.00% 42 2 0.00% 43 2 0.00% 44 2 0.00% 45 5 0.00% 46 2 0.00% 47 6 0.00% 48 3 0.00% 49 541 0.00% 50 558 0.00% 51 639 0.00% 52 740 0.00% 53 684 0.00% 54 745 0.00% 55 858 0.00% 56 959 0.00% 57 1118 0.00% 58 1258 0.00% 59 1404 0.00% 60 1549 0.00% 61 1857 0.00% 62 2017 0.01% 63 2231 0.01% 64 2388 0.01% 65 2606 0.01% 66 2875 0.01% 67 3223 0.01% 68 3593 0.01% 69 4284 0.01% 70 4848 0.01% 71 5388 0.01% 72 6194 0.02% 73 7010 0.02% 74 7564 0.02% 75 8076 0.02% 76 8695 0.02% 77 9348 0.02% 78 10220 0.03% 79 11455 0.03% 80 12600 0.03% 81 14291 0.04% 82 15733 0.04% 83 17353 0.05% 84 18443 0.05% 85 20100 0.05% 86 20710 0.06% 87 21582 0.06% 88 22742 0.06% 89 24074 0.06% 90 26533 0.07% 91 28270 0.08% 92 30540 0.08% 93 33217 0.09% 94 35691 0.09% 95 36226 0.10% 96 37984 0.10% 97 38937 0.10% 98 40480 0.11% 99 41847 0.11% 100 44105 0.12% 101 46376 0.12% 102 49103 0.13% 103 52245 0.14% 104 54138 0.14% 105 56592 0.15% 106 57923 0.15% 107 58991 0.16% 108 60445 0.16% 109 61601 0.16% 110 63353 0.17% 111 66406 0.18% 112 70033 0.19% 113 72724 0.19% 114 76474 0.20% 115 78377 0.21% 116 80664 0.21% 117 81284 0.22% 118 81963 0.22% 119 83298 0.22% 120 84907 0.23% 121 87214 0.23% 122 89799 0.24% 123 94052 0.25% 124 98521 0.26% 125 100860 0.27% 126 102110 0.27% 127 102562 0.27% 128 103616 0.28% 129 104836 0.28% 130 104724 0.28% 131 107710 0.29% 132 109926 0.29% 133 114813 0.31% 134 117256 0.31% 135 120866 0.32% 136 122054 0.32% 137 122592 0.33% 138 123622 0.33% 139 128293 0.34% 140 133689 0.36% 141 141027 0.37% 142 146592 0.39% 143 149897 0.40% 144 156481 0.42% 145 151569 0.40% 146 158442 0.42% 147 334480 0.89% 148 136253 0.36% 149 135161 0.36% 150 31899440 84.77% 37631809 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=15.24 fanout-score-rank=24 prefix-density=0.78 prefix-fanout=5.1 sequence=AGCTTCTTCAGGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=153.51 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=7.7 sequence=ACAAACAAATCTCTTATTAGAGACATTCAGAGAGGTTTCCACAAATCCCAGATGGTGGTTTCTCGAGTCAATTTTTCCAAAACAATGCAAATCAAGAGTGGGGCCGCTGCTGGTTTTCTCCAGTAAGAGTTTCGAAGTTTCAGTGGCTTCAGACTGGCCCTTGTTTGAATAGTTTCAAGCCTATTTACTGCCTCTCCAAACGCAGCAGCAAT criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=3.64 fanout-score-rank=30 prefix-density=0.40 prefix-fanout=3.0 sequence=AGCAGAGCAGAG criterion=fanout-score sequence-density=0.11 sequence-density-rank=18 fanout-score=147.71 fanout-score-rank=1 prefix-density=0.86 prefix-fanout=18.4 sequence=CGCCGCCGCCGTC ERR5262813 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:32:26 Started mapping on | Dec 06 12:32:27 Finished on | Dec 06 12:34:56 Mapping speed, Million of reads per hour | 909.22 Number of input reads | 37631809 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 35719423 Uniquely mapped reads % | 94.92% Average mapped length | 291.29 Number of splices: Total | 31381997 Number of splices: Annotated (sjdb) | 29135771 Number of splices: GT/AG | 30952541 Number of splices: GC/AG | 355089 Number of splices: AT/AC | 18542 Number of splices: Non-canonical | 55825 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.37 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 357309 % of reads mapped to multiple loci | 0.95% Number of reads mapped to too many loci | 1185 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.12% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1555077 1555077 1555077 N_multimapping 357309 357309 357309 N_noFeature 1199188 34771388 1497521 N_ambiguous 764460 4613 114733 UnstrandedReadsAssigned:33755775 PositiveStrandReadsAssigned:943422 NegativeStrandReadsAssigned:34107169 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262813 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262813-trimmed-pair1.fastq ERR5262813-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 37,631,809 reads, 34,759,912 reads pseudoaligned [quant] estimated average fragment length: 267.269 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,198 rounds 52973 ERR5262813.ke.tsv 35125 ERR5262813.se.tsv 88098 total ==> ERR5262813.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 670.5 0 0 PNS24247 1044 777.731 214.963 11.1109 PNS24249 1928 1661.73 610.34 14.7648 PNS24246 1044 777.731 214.963 11.1109 PNS24248 1044 777.731 214.963 11.1109 PNS24244 1471 1204.73 258.77 8.63454 PNS24243 293 102.072 0 0 KQK14069 1603 1336.73 29593.6 889.958 KQK14071 474 238.348 867.653 146.335 ==> ERR5262813.se.tsv <== BRADI_1g14170v3 32750 BRADI_1g53295v3 185 BRADI_1g59795v3 590 BRADI_1g07683v3 0 BRADI_1g00485v3 36 BRADI_1g20270v3 1272 BRADI_1g74790v3 2454 BRADI_1g09890v3 0 BRADI_1g77505v3 297 BRADI_1g48960v3 0 ERR5262813 completed mapping pipeline successfully