Starting /dee2/code/volunteer_pipeline.sh ERR6133366 current disk space = 1548113465344 free memory = 1433219156 ERR6133366 SRAfilesize a873c61741df5b9fd4d58a348b48568a ERR6133366.sra ERR6133366.sra file validated ERR6133366 is single end ERR6133366 is conventional basespace ERR6133366 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133366_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.89325 37.0 37.0 37.0 37.0 37.0 2 36.79025 37.0 37.0 37.0 37.0 37.0 3 36.58575 37.0 37.0 37.0 37.0 37.0 4 36.144 37.0 37.0 37.0 33.0 37.0 5 36.1935 37.0 37.0 37.0 33.0 37.0 6 36.29925 37.0 37.0 37.0 37.0 37.0 7 38.309 40.0 37.0 40.0 37.0 40.0 8 38.238 40.0 37.0 40.0 37.0 40.0 9 38.24125 40.0 37.0 40.0 37.0 40.0 10-11 38.199 40.0 37.0 40.0 37.0 40.0 12-13 38.224625 40.0 37.0 40.0 37.0 40.0 14-15 38.144999999999996 40.0 37.0 40.0 37.0 40.0 16-17 38.02375 40.0 37.0 40.0 35.0 40.0 18-19 37.906875 40.0 37.0 40.0 33.0 40.0 20-21 37.8155 40.0 37.0 40.0 33.0 40.0 22-23 37.762375000000006 40.0 37.0 40.0 33.0 40.0 24-25 37.620374999999996 40.0 37.0 40.0 33.0 40.0 26-27 37.537125 37.0 37.0 40.0 33.0 40.0 28-29 37.3655 37.0 37.0 40.0 33.0 40.0 30-31 37.258875 37.0 37.0 40.0 33.0 40.0 32-33 37.080875 37.0 37.0 40.0 33.0 40.0 34-35 36.859875 37.0 37.0 40.0 33.0 40.0 36-37 36.824124999999995 37.0 37.0 40.0 33.0 40.0 38-39 36.85625 37.0 37.0 40.0 33.0 40.0 40-41 37.138125 37.0 37.0 40.0 33.0 40.0 42-43 37.111125 37.0 37.0 40.0 33.0 40.0 44-45 37.00925 37.0 37.0 40.0 33.0 40.0 46-47 36.739000000000004 37.0 37.0 40.0 33.0 40.0 48-49 36.585125000000005 37.0 37.0 40.0 33.0 40.0 50-51 36.436875 37.0 37.0 40.0 33.0 40.0 52-53 36.38849999999999 37.0 37.0 37.0 33.0 40.0 54-55 36.184124999999995 37.0 37.0 37.0 33.0 40.0 56-57 35.93725 37.0 37.0 37.0 33.0 40.0 58-59 35.816375 37.0 37.0 37.0 33.0 40.0 60-61 35.659875 37.0 35.0 37.0 33.0 38.5 62-63 35.318749999999994 37.0 33.0 37.0 33.0 37.0 64-65 35.1465 37.0 33.0 37.0 33.0 37.0 66-67 35.13475 37.0 33.0 37.0 33.0 37.0 68-69 34.057249999999996 35.0 33.0 37.0 30.0 37.0 70-71 34.217320611402855 35.0 33.0 37.0 30.0 37.0 72-73 34.789715103672236 37.0 33.0 37.0 33.0 37.0 74-75 34.78005307699258 37.0 33.0 37.0 33.0 37.0 76-77 34.60852130129581 37.0 33.0 37.0 33.0 37.0 78-79 34.751366769547715 37.0 33.0 37.0 33.0 37.0 80-81 34.700857985656924 37.0 33.0 37.0 33.0 37.0 82-83 34.3374027199349 37.0 33.0 37.0 33.0 37.0 84-85 34.30494031004979 37.0 33.0 37.0 33.0 37.0 86-87 34.1224750703145 37.0 33.0 37.0 27.0 37.0 88-89 34.085527997954486 37.0 33.0 37.0 33.0 37.0 90-91 33.882638711327026 37.0 33.0 37.0 27.0 37.0 92-93 33.68090002556891 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 6.0 21 14.0 22 7.0 23 13.0 24 14.0 25 15.0 26 18.0 27 34.0 28 31.0 29 43.0 30 56.0 31 76.0 32 96.0 33 133.0 34 203.0 35 378.0 36 819.0 37 1050.0 38 961.0 39 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 80.375 5.7250000000000005 4.3999999999999995 9.5 2 57.99999999999999 24.2 11.275 6.525 3 33.324999999999996 35.75 17.474999999999998 13.450000000000001 4 33.375 25.6 20.575 20.45 5 23.549999999999997 31.25 26.1 19.1 6 19.8 37.125 25.1 17.974999999999998 7 36.475 28.349999999999998 20.25 14.924999999999999 8 31.1 26.55 24.25 18.099999999999998 9 25.424999999999997 26.424999999999997 28.549999999999997 19.6 10-11 25.7375 27.3125 26.724999999999998 20.225 12-13 28.95 24.1375 28.175 18.7375 14-15 22.225 26.5875 30.6375 20.549999999999997 16-17 25.4375 30.125 25.924999999999997 18.512500000000003 18-19 25.162499999999998 25.124999999999996 26.7125 23.0 20-21 26.9625 25.124999999999996 27.3875 20.525 22-23 26.237500000000004 24.15 28.325 21.2875 24-25 26.400000000000002 23.962500000000002 27.025 22.6125 26-27 25.3 25.2125 29.012500000000003 20.474999999999998 28-29 25.7 25.912499999999998 27.3 21.087500000000002 30-31 27.237499999999997 24.675 26.924999999999997 21.1625 32-33 24.6625 26.437500000000004 27.6125 21.2875 34-35 25.525 24.775 27.750000000000004 21.95 36-37 25.3 24.087500000000002 27.5875 23.025000000000002 38-39 25.637500000000003 24.2875 30.012499999999996 20.0625 40-41 26.075 24.837500000000002 28.199999999999996 20.8875 42-43 25.0 26.700000000000003 26.7625 21.5375 44-45 24.25 25.650000000000002 28.6625 21.4375 46-47 25.1875 23.5 28.199999999999996 23.1125 48-49 26.375 24.2625 29.1125 20.25 50-51 25.35 25.35 28.199999999999996 21.099999999999998 52-53 24.575 25.587500000000002 26.825 23.0125 54-55 24.05 26.487500000000004 28.1125 21.349999999999998 56-57 24.9875 25.687500000000004 28.225 21.099999999999998 58-59 24.7 24.7 28.275 22.325 60-61 25.75 24.275 28.462500000000002 21.512500000000003 62-63 24.025 26.387500000000003 29.1875 20.4 64-65 24.762500000000003 25.95 27.962500000000002 21.325 66-67 25.650000000000002 25.9875 28.175 20.1875 68-69 23.625 25.5375 29.2 21.637500000000003 70-71 25.57819727465933 25.453181647705964 27.69096137017127 21.277659707463435 72-73 26.261740763932373 24.871634314339385 27.977457733249842 20.889167188478396 74-75 23.73286379071815 26.462080241479057 28.876870833857378 20.928185133945416 76-77 23.41364955216349 25.5834489718683 29.65813044026744 21.34477103570077 78-79 24.430379746835442 24.645569620253163 30.050632911392405 20.87341772151899 80-81 25.101574403250382 26.701371254443877 29.291518537328592 18.90553580497715 82-83 24.726254138018845 25.515660809778456 29.475426534250065 20.282658517952637 84-85 24.121630254248117 24.77322090200588 30.407563562028873 20.69758528171713 86-87 22.769112758885196 26.681155714650984 30.31194068013296 20.23779084633086 88-89 23.446688826387113 27.959601125031963 29.74942469956533 18.844285349015596 90-91 25.50498593710049 26.208130912810024 28.598823830222447 19.68805931986704 92-93 22.628483763743287 27.93403221682434 29.890053694707237 19.547430324725134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 9.0 18 10.0 19 1.5 20 0.5 21 1.5 22 3.0 23 3.0 24 3.5 25 5.0 26 8.5 27 12.5 28 12.5 29 11.5 30 18.0 31 25.5 32 36.0 33 49.0 34 71.0 35 95.0 36 103.5 37 118.0 38 127.5 39 138.5 40 162.0 41 180.0 42 192.5 43 198.0 44 202.0 45 194.5 46 219.5 47 222.5 48 179.5 49 169.5 50 179.0 51 165.5 52 152.0 53 164.5 54 148.0 55 106.0 56 77.0 57 70.0 58 70.0 59 55.5 60 41.0 61 37.5 62 38.5 63 41.0 64 40.0 65 35.5 66 22.5 67 17.0 68 18.0 69 13.5 70 9.0 71 8.0 72 6.5 73 4.0 74 3.5 75 3.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 1.0 71 3.0 72 7.0 73 10.0 74 7.0 75 6.0 76 5.0 77 7.0 78 8.0 79 5.0 80 6.0 81 6.0 82 4.0 83 9.0 84 5.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3911.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.1 #Duplication Level Percentage of deduplicated Percentage of total 1 94.9483352468427 82.69999999999999 2 3.329506314580941 5.800000000000001 3 0.5453501722158438 1.425 4 0.3731343283582089 1.3 5 0.20091848450057406 0.8750000000000001 6 0.08610792192881744 0.44999999999999996 7 0.1148105625717566 0.7000000000000001 8 0.0 0.0 9 0.02870264064293915 0.22499999999999998 >10 0.3731343283582089 6.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 44 1.0999999999999999 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 43 1.075 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 25 0.625 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 21 0.525 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 19 0.475 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 17 0.42500000000000004 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 17 0.42500000000000004 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 15 0.375 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 14 0.35000000000000003 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 13 0.325 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 12 0.3 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 11 0.27499999999999997 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 10 0.25 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 9 0.22499999999999998 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 7 0.17500000000000002 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 7 0.17500000000000002 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 7 0.17500000000000002 No Hit GATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAG 7 0.17500000000000002 No Hit GGTCTTGATCCCTCTGTGTTTCCCGTGTAACGGCTACTGATCCAGTGGTT 6 0.15 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 6 0.15 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 6 0.15 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 5 0.125 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 5 0.125 No Hit CTGAGCCGTGCTCTCTTCTGTGTACATCTCATGTCATTTTAGCGATTCTC 5 0.125 No Hit GATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGGG 5 0.125 No Hit AACAGTAAAGCTTCATAGGGTCTTTCTGTCCAGGTGCAGGTAGTCCGCAT 5 0.125 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 5 0.125 No Hit AAATCATATGGTCGTACTGTAAATCTTTTGTGTCTAGTATAATTCTATAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTTGG 30 0.005906258 28.816666 44-45 CTGTATT 30 0.005906258 28.816666 8 >>END_MODULE Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84925 READS because READLEN < 1 Read 84925 spots for ERR6133366.sra Written 84925 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra Rejected 84920 READS because READLEN < 1 Read 84920 spots for ERR6133366.sra Written 84920 spots for ERR6133366.sra SRR ids: ['ERR6133366.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_72vgdhnz ERR6133366.sra spots: 1698405 blocks: [[1, 84920], [84921, 169840], [169841, 254760], [254761, 339680], [339681, 424600], [424601, 509520], [509521, 594440], [594441, 679360], [679361, 764280], [764281, 849200], [849201, 934120], [934121, 1019040], [1019041, 1103960], [1103961, 1188880], [1188881, 1273800], [1273801, 1358720], [1358721, 1443640], [1443641, 1528560], [1528561, 1613480], [1613481, 1698405]] ERR6133366 file size 374406 ERR6133366 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133366 ERR6133366_1.fastq Input file: ERR6133366_1.fastq trimmed: ERR6133366-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 01:29:38 2024 >> started Sat Dec 7 01:29:39 2024 >> done (1.181s) 1698405 reads processed; of these: 44 ( 0.00%) short reads filtered out after trimming by size control 1 ( 0.00%) empty reads filtered out after trimming by size control 1698360 (100.00%) reads available; of these: 73293 ( 4.32%) trimmed reads available after processing 1625067 (95.68%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 6 0.00% 20 3 0.00% 21 3 0.00% 22 3 0.00% 23 4 0.00% 24 7 0.00% 25 1 0.00% 26 2 0.00% 27 4 0.00% 28 2 0.00% 29 67 0.00% 30 2 0.00% 31 4 0.00% 32 1 0.00% 33 2 0.00% 34 4 0.00% 35 2 0.00% 36 2 0.00% 37 2 0.00% 38 4 0.00% 39 7 0.00% 40 16 0.00% 41 6 0.00% 42 9 0.00% 43 7 0.00% 44 13 0.00% 45 13 0.00% 46 42 0.00% 47 45 0.00% 48 87 0.01% 49 535 0.03% 50 161 0.01% 51 143 0.01% 52 192 0.01% 53 179 0.01% 54 219 0.01% 55 280 0.02% 56 284 0.02% 57 341 0.02% 58 338 0.02% 59 369 0.02% 60 515 0.03% 61 440 0.03% 62 518 0.03% 63 660 0.04% 64 558 0.03% 65 594 0.03% 66 677 0.04% 67 740 0.04% 68 899 0.05% 69 420 0.02% 70 3429 0.20% 71 3691 0.22% 72 4347 0.26% 73 4148 0.24% 74 4160 0.24% 75 4521 0.27% 76 4054 0.24% 77 4195 0.25% 78 4775 0.28% 79 4952 0.29% 80 5010 0.29% 81 5153 0.30% 82 5952 0.35% 83 6716 0.40% 84 6193 0.36% 85 2999 0.18% 86 3420 0.20% 87 3925 0.23% 88 5075 0.30% 89 5077 0.30% 90 6638 0.39% 91 7420 0.44% 92 8228 0.48% 93 1574846 92.73% 1698360 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=10.04 fanout-score-rank=9 prefix-density=0.61 prefix-fanout=5.4 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=26 fanout-score=61.40 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=6.8 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTATAGGGAAAACATAATTTATGAGACAATCATACTGTGCAACTCTTGGCTCCATCGATTAATTAATACTCCTTGTTATTGCTTGCATGGT Started job on | Dec 07 01:29:57 Started mapping on | Dec 07 01:29:57 Finished on | Dec 07 01:30:04 Mapping speed, Million of reads per hour | 873.44 Number of input reads | 1698360 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 1307382 Uniquely mapped reads % | 76.98% Average mapped length | 91.65 Number of splices: Total | 80802 Number of splices: Annotated (sjdb) | 68700 Number of splices: GT/AG | 77801 Number of splices: GC/AG | 1602 Number of splices: AT/AC | 23 Number of splices: Non-canonical | 1376 Mismatch rate per base, % | 0.49% Deletion rate per base | 0.04% Deletion average length | 1.82 Insertion rate per base | 0.02% Insertion average length | 1.76 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 312420 % of reads mapped to multiple loci | 18.40% Number of reads mapped to too many loci | 14314 % of reads mapped to too many loci | 0.84% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.66% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 78558 78558 78558 N_multimapping 312420 312420 312420 N_noFeature 60687 73596 1249276 N_ambiguous 48981 3828 125 UnstrandedReadsAssigned:1197714 PositiveStrandReadsAssigned:1229958 NegativeStrandReadsAssigned:57981 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133366 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133366-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,698,360 reads, 1,422,937 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 936 rounds 52973 ERR6133366.ke.tsv 35125 ERR6133366.se.tsv 88098 total ==> ERR6133366.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 51 35.2765 PNS24243 293 194 0 0 KQK14069 1603 1504 14 8.83384 KQK14071 474 375 0 0 ==> ERR6133366.se.tsv <== BRADI_1g14170v3 14 BRADI_1g53295v3 9 BRADI_1g59795v3 5 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 11 BRADI_1g74790v3 20 BRADI_1g09890v3 0 BRADI_1g77505v3 36 BRADI_1g48960v3 0 ERR6133366 completed mapping pipeline successfully