Starting /dee2/code/volunteer_pipeline.sh ERR6133367 current disk space = 1548137955328 free memory = 1597128528 ERR6133367 SRAfilesize 7c74a18e8d4102dae5e45bd444b7386c ERR6133367.sra ERR6133367.sra file validated ERR6133367 is single end ERR6133367 is conventional basespace ERR6133367 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133367_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.88425 37.0 37.0 37.0 37.0 37.0 2 36.76175 37.0 37.0 37.0 37.0 37.0 3 36.545 37.0 37.0 37.0 37.0 37.0 4 36.0345 37.0 37.0 37.0 33.0 37.0 5 36.029 37.0 37.0 37.0 33.0 37.0 6 36.22075 37.0 37.0 37.0 33.0 37.0 7 38.22275 40.0 37.0 40.0 37.0 40.0 8 38.16525 40.0 37.0 40.0 37.0 40.0 9 38.16225 40.0 37.0 40.0 37.0 40.0 10-11 38.167874999999995 40.0 37.0 40.0 37.0 40.0 12-13 38.23675 40.0 37.0 40.0 37.0 40.0 14-15 38.144125 40.0 37.0 40.0 37.0 40.0 16-17 38.094625 40.0 37.0 40.0 35.0 40.0 18-19 38.03375 40.0 37.0 40.0 33.0 40.0 20-21 37.810500000000005 40.0 37.0 40.0 33.0 40.0 22-23 37.798625 40.0 37.0 40.0 33.0 40.0 24-25 37.710125 40.0 37.0 40.0 33.0 40.0 26-27 37.504125 40.0 37.0 40.0 33.0 40.0 28-29 37.4385 38.5 37.0 40.0 33.0 40.0 30-31 37.339875 37.0 37.0 40.0 33.0 40.0 32-33 37.013875 37.0 37.0 40.0 33.0 40.0 34-35 36.910375 37.0 37.0 40.0 33.0 40.0 36-37 36.913125 37.0 37.0 40.0 33.0 40.0 38-39 37.07275 37.0 37.0 40.0 33.0 40.0 40-41 37.252875 37.0 37.0 40.0 33.0 40.0 42-43 37.14 37.0 37.0 40.0 33.0 40.0 44-45 37.082750000000004 37.0 37.0 40.0 33.0 40.0 46-47 36.83425 37.0 37.0 40.0 33.0 40.0 48-49 36.763 37.0 37.0 40.0 33.0 40.0 50-51 36.6085 37.0 37.0 40.0 33.0 40.0 52-53 36.44499999999999 37.0 37.0 37.0 33.0 40.0 54-55 36.332 37.0 37.0 37.0 33.0 40.0 56-57 36.013875 37.0 37.0 37.0 33.0 40.0 58-59 35.946375 37.0 37.0 37.0 33.0 40.0 60-61 35.809875 37.0 37.0 37.0 33.0 38.5 62-63 35.52675 37.0 35.0 37.0 33.0 37.0 64-65 35.252125 37.0 33.0 37.0 33.0 37.0 66-67 35.248125 37.0 33.0 37.0 33.0 37.0 68-69 34.305625 35.0 33.0 37.0 30.0 37.0 70-71 34.50119384692346 37.0 33.0 37.0 30.0 37.0 72-73 34.90268608577968 37.0 33.0 37.0 33.0 37.0 74-75 34.993708509238076 37.0 33.0 37.0 33.0 37.0 76-77 34.80811841478523 37.0 33.0 37.0 33.0 37.0 78-79 34.95985782759503 37.0 33.0 37.0 33.0 37.0 80-81 34.825554071670965 37.0 33.0 37.0 33.0 37.0 82-83 34.40375448492232 37.0 33.0 37.0 33.0 37.0 84-85 34.504758972137815 37.0 33.0 37.0 33.0 37.0 86-87 34.48150078165712 37.0 33.0 37.0 33.0 37.0 88-89 34.40424700364773 37.0 33.0 37.0 33.0 37.0 90-91 34.01693590411673 37.0 33.0 37.0 27.0 37.0 92-93 33.866336633663366 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 8.0 21 3.0 22 13.0 23 13.0 24 16.0 25 16.0 26 17.0 27 29.0 28 33.0 29 41.0 30 57.0 31 64.0 32 94.0 33 112.0 34 190.0 35 331.0 36 844.0 37 1062.0 38 1018.0 39 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 82.6 3.8249999999999997 4.2 9.375 2 59.099999999999994 23.799999999999997 11.0 6.1 3 33.4 36.65 16.425 13.525 4 34.475 25.900000000000002 18.925 20.7 5 21.925 31.900000000000002 25.55 20.625 6 20.275000000000002 37.85 26.05 15.825 7 35.675000000000004 29.925 18.875 15.525 8 29.775000000000002 29.5 24.7 16.025 9 25.75 26.200000000000003 29.325000000000003 18.725 10-11 24.625 27.962500000000002 27.675 19.7375 12-13 27.525 24.9875 29.475 18.0125 14-15 22.25 25.650000000000002 31.9875 20.1125 16-17 25.45 30.6875 25.8 18.0625 18-19 23.1875 26.6625 26.625 23.525 20-21 25.05 26.150000000000002 28.537499999999998 20.2625 22-23 26.5125 24.3625 28.287499999999998 20.837500000000002 24-25 25.8125 24.825 28.9125 20.45 26-27 25.324999999999996 24.6125 30.337500000000002 19.725 28-29 25.15 25.6125 28.4375 20.8 30-31 27.125 25.525 26.950000000000003 20.4 32-33 23.9125 27.500000000000004 29.275000000000002 19.3125 34-35 25.087500000000002 25.074999999999996 28.325 21.512500000000003 36-37 24.5625 24.224999999999998 29.675 21.5375 38-39 25.324999999999996 25.275 29.2375 20.1625 40-41 25.424999999999997 25.687500000000004 28.237499999999997 20.65 42-43 24.3125 27.525 27.8875 20.275000000000002 44-45 23.3625 24.7875 30.312499999999996 21.5375 46-47 25.2 24.349999999999998 28.475 21.975 48-49 25.124999999999996 24.962500000000002 29.2 20.7125 50-51 25.112499999999997 25.112499999999997 29.062500000000004 20.7125 52-53 25.4 26.437500000000004 27.6125 20.549999999999997 54-55 25.074999999999996 26.125 28.525 20.275000000000002 56-57 24.25 26.325 28.7375 20.6875 58-59 23.3 24.762500000000003 30.075000000000003 21.8625 60-61 24.1875 25.6125 29.825000000000003 20.375 62-63 22.662499999999998 28.15 30.175 19.0125 64-65 23.6375 26.337500000000002 29.299999999999997 20.724999999999998 66-67 24.212500000000002 27.3125 29.6375 18.8375 68-69 23.8625 26.700000000000003 28.375 21.0625 70-71 25.64391097774444 25.09377344336084 28.95723930982746 20.305076269067268 72-73 24.789652141152832 26.058018334798444 29.05939972372222 20.09292980032651 74-75 24.117053481331986 26.034308779011102 29.805751765893035 20.042885973763873 76-77 22.522179974651458 26.4638783269962 30.228136882129274 20.785804816223067 78-79 24.151569277877012 25.606021944373563 30.35213064557285 19.890278132176576 80-81 23.981493381313456 28.042668037527307 29.186479886904 18.789358694255238 82-83 23.911639323084874 24.89342462214184 30.293243766955175 20.90169228781811 84-85 23.322060353798125 24.81789802289282 30.619146722164416 21.240894901144642 86-87 22.45961438249088 26.146430432516937 31.20114643043252 20.192808754559664 88-89 23.00677436164669 28.895257946847313 30.263157894736842 17.83480979676915 90-91 24.166232412714955 26.941115164147995 30.250130276185512 18.64252214695154 92-93 21.912454403335072 27.892131318395 30.953621677957273 19.241792600312664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 5.0 18 6.0 19 4.5 20 3.5 21 2.5 22 3.5 23 3.5 24 4.5 25 5.5 26 6.5 27 11.5 28 20.5 29 24.0 30 28.0 31 43.0 32 56.5 33 65.5 34 91.0 35 115.5 36 119.0 37 137.5 38 163.5 39 175.5 40 190.0 41 196.5 42 185.0 43 176.5 44 188.0 45 197.0 46 200.5 47 193.0 48 181.0 49 174.0 50 165.5 51 156.5 52 143.0 53 139.5 54 126.0 55 89.0 56 67.5 57 68.0 58 65.0 59 45.5 60 33.0 61 39.5 62 33.0 63 33.5 64 36.5 65 24.5 66 20.5 67 16.5 68 9.5 69 7.0 70 4.0 71 2.0 72 3.5 73 2.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 2.0 71 9.0 72 15.0 73 7.0 74 6.0 75 10.0 76 12.0 77 15.0 78 10.0 79 17.0 80 13.0 81 9.0 82 9.0 83 16.0 84 12.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3838.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.55 #Duplication Level Percentage of deduplicated Percentage of total 1 95.03141062250144 83.2 2 3.026841804683038 5.3 3 0.7709880068532268 2.025 4 0.2284408909194746 0.8 5 0.2284408909194746 1.0 6 0.1427755568246716 0.75 7 0.08566533409480297 0.525 8 0.08566533409480297 0.6 9 0.028555111364934323 0.22499999999999998 >10 0.37121644774414625 5.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 37 0.9249999999999999 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 24 0.6 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 24 0.6 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 19 0.475 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 18 0.44999999999999996 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 14 0.35000000000000003 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 14 0.35000000000000003 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 14 0.35000000000000003 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 14 0.35000000000000003 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 13 0.325 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 11 0.27499999999999997 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 11 0.27499999999999997 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 10 0.25 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 9 0.22499999999999998 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 8 0.2 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 8 0.2 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 8 0.2 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 7 0.17500000000000002 No Hit GGGACGCATGCAACGACCATCTACATATAGCTACTCGATCTACCGCTACC 7 0.17500000000000002 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 7 0.17500000000000002 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 6 0.15 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 6 0.15 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 6 0.15 No Hit GGGCCTGTTATCTCTATCAATATGATTCTAATTCGTCAGATATTATTTAT 6 0.15 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 6 0.15 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 5 0.125 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 5 0.125 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 5 0.125 No Hit TAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTTGACGGCGGACT 5 0.125 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 5 0.125 No Hit GGGAAATTAACAGTTGGAAAGGGCGATCGGTCTTGATCCCTCTGTGTTTC 5 0.125 No Hit GGGTGTGAGCTGGAGAGACGATCGGGTCTCTCAGCCGGCGTCTTCATCAG 5 0.125 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGTGC 20 8.0731796E-4 43.225 12-13 ACCTGGT 25 0.002418236 34.579998 10-11 GGTGCAG 25 0.002418236 34.579998 14-15 >>END_MODULE Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204273 READS because READLEN < 1 Read 204273 spots for ERR6133367.sra Written 204273 spots for ERR6133367.sra Rejected 204277 READS because READLEN < 1 Read 204277 spots for ERR6133367.sra Written 204277 spots for ERR6133367.sra SRR ids: ['ERR6133367.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_fucubpb0 ERR6133367.sra spots: 4085464 blocks: [[1, 204273], [204274, 408546], [408547, 612819], [612820, 817092], [817093, 1021365], [1021366, 1225638], [1225639, 1429911], [1429912, 1634184], [1634185, 1838457], [1838458, 2042730], [2042731, 2247003], [2247004, 2451276], [2451277, 2655549], [2655550, 2859822], [2859823, 3064095], [3064096, 3268368], [3268369, 3472641], [3472642, 3676914], [3676915, 3881187], [3881188, 4085464]] ERR6133367 file size 902109 ERR6133367 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133367 ERR6133367_1.fastq Input file: ERR6133367_1.fastq trimmed: ERR6133367-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 01:32:40 2024 >> started Sat Dec 7 01:32:44 2024 >> done (3.786s) 4085464 reads processed; of these: 152 ( 0.00%) short reads filtered out after trimming by size control 0 ( 0.00%) empty reads filtered out after trimming by size control 4085312 (100.00%) reads available; of these: 161512 ( 3.95%) trimmed reads available after processing 3923800 (96.05%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 33 0.00% 20 11 0.00% 21 11 0.00% 22 11 0.00% 23 8 0.00% 24 7 0.00% 25 11 0.00% 26 10 0.00% 27 10 0.00% 28 9 0.00% 29 106 0.00% 30 4 0.00% 31 9 0.00% 32 18 0.00% 33 7 0.00% 34 7 0.00% 35 8 0.00% 36 8 0.00% 37 13 0.00% 38 12 0.00% 39 45 0.00% 40 55 0.00% 41 18 0.00% 42 23 0.00% 43 18 0.00% 44 24 0.00% 45 42 0.00% 46 66 0.00% 47 114 0.00% 48 234 0.01% 49 1404 0.03% 50 379 0.01% 51 413 0.01% 52 415 0.01% 53 427 0.01% 54 522 0.01% 55 619 0.02% 56 668 0.02% 57 804 0.02% 58 742 0.02% 59 904 0.02% 60 1313 0.03% 61 1010 0.02% 62 1056 0.03% 63 1428 0.03% 64 1253 0.03% 65 1345 0.03% 66 1487 0.04% 67 1498 0.04% 68 2108 0.05% 69 986 0.02% 70 11537 0.28% 71 12086 0.30% 72 14120 0.35% 73 12798 0.31% 74 12836 0.31% 75 13710 0.34% 76 12556 0.31% 77 12711 0.31% 78 13980 0.34% 79 15069 0.37% 80 14634 0.36% 81 15091 0.37% 82 17183 0.42% 83 20076 0.49% 84 17583 0.43% 85 6518 0.16% 86 7422 0.18% 87 8506 0.21% 88 11013 0.27% 89 11015 0.27% 90 14787 0.36% 91 16576 0.41% 92 18167 0.44% 93 3753591 91.88% 4085312 reads passed initial QC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=6.28 fanout-score-rank=24 prefix-density=0.80 prefix-fanout=4.3 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=17 fanout-score=118.04 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=19.8 sequence=TTTTTTTTTAAGAATCCTCCATTTTTGTTCTTCCACCCATGCAATAGAGAGCAAATGGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGAGTCATGGAATAATGGTGAATTCAAATGTTTAGTTCTTTAGTATAAGAAGTATAAGAA Started job on | Dec 07 01:32:59 Started mapping on | Dec 07 01:32:59 Finished on | Dec 07 01:33:07 Mapping speed, Million of reads per hour | 1838.39 Number of input reads | 4085312 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 3170006 Uniquely mapped reads % | 77.60% Average mapped length | 91.53 Number of splices: Total | 156203 Number of splices: Annotated (sjdb) | 132022 Number of splices: GT/AG | 149748 Number of splices: GC/AG | 3204 Number of splices: AT/AC | 52 Number of splices: Non-canonical | 3199 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.03% Deletion average length | 1.77 Insertion rate per base | 0.02% Insertion average length | 1.82 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 774170 % of reads mapped to multiple loci | 18.95% Number of reads mapped to too many loci | 44565 % of reads mapped to too many loci | 1.09% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.22% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 141136 141136 141136 N_multimapping 774170 774170 774170 N_noFeature 162245 190294 3029947 N_ambiguous 123780 11805 368 UnstrandedReadsAssigned:2883981 PositiveStrandReadsAssigned:2967907 NegativeStrandReadsAssigned:139691 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133367 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133367-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 4,085,312 reads, 3,447,478 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,063 rounds 52973 ERR6133367.ke.tsv 35125 ERR6133367.se.tsv 88098 total ==> ERR6133367.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 93 26.7083 PNS24243 293 194 0 0 KQK14069 1603 1504 68 17.8147 KQK14071 474 375 0 0 ==> ERR6133367.se.tsv <== BRADI_1g14170v3 68 BRADI_1g53295v3 37 BRADI_1g59795v3 26 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 51 BRADI_1g74790v3 43 BRADI_1g09890v3 0 BRADI_1g77505v3 107 BRADI_1g48960v3 0 ERR6133367 completed mapping pipeline successfully