Starting /dee2/code/volunteer_pipeline.sh ERR6133368 current disk space = 1548064022528 free memory = 1359231876 ERR6133368 SRAfilesize c4093a3a9dce49d3a46109e4b483ea25 ERR6133368.sra ERR6133368.sra file validated ERR6133368 is single end ERR6133368 is conventional basespace ERR6133368 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133368_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.887 37.0 37.0 37.0 37.0 37.0 2 36.78775 37.0 37.0 37.0 37.0 37.0 3 36.56875 37.0 37.0 37.0 37.0 37.0 4 36.06 37.0 37.0 37.0 33.0 37.0 5 36.10675 37.0 37.0 37.0 33.0 37.0 6 36.27 37.0 37.0 37.0 33.0 37.0 7 38.23825 40.0 37.0 40.0 37.0 40.0 8 38.1595 40.0 37.0 40.0 37.0 40.0 9 38.19575 40.0 37.0 40.0 37.0 40.0 10-11 38.144 40.0 37.0 40.0 37.0 40.0 12-13 38.184 40.0 37.0 40.0 37.0 40.0 14-15 38.1205 40.0 37.0 40.0 37.0 40.0 16-17 38.072874999999996 40.0 37.0 40.0 35.0 40.0 18-19 37.9845 40.0 37.0 40.0 33.0 40.0 20-21 37.79075 40.0 37.0 40.0 33.0 40.0 22-23 37.859875 40.0 37.0 40.0 33.0 40.0 24-25 37.707375 40.0 37.0 40.0 33.0 40.0 26-27 37.60025 40.0 37.0 40.0 33.0 40.0 28-29 37.489000000000004 37.0 37.0 40.0 33.0 40.0 30-31 37.33775 37.0 37.0 40.0 33.0 40.0 32-33 37.233374999999995 37.0 37.0 40.0 33.0 40.0 34-35 37.027249999999995 37.0 37.0 40.0 33.0 40.0 36-37 36.920249999999996 37.0 37.0 40.0 33.0 40.0 38-39 37.0325 37.0 37.0 40.0 33.0 40.0 40-41 37.308625 37.0 37.0 40.0 33.0 40.0 42-43 37.24825 37.0 37.0 40.0 33.0 40.0 44-45 37.166624999999996 37.0 37.0 40.0 33.0 40.0 46-47 36.932875 37.0 37.0 40.0 33.0 40.0 48-49 36.816625 37.0 37.0 40.0 33.0 40.0 50-51 36.678875 37.0 37.0 40.0 33.0 40.0 52-53 36.518249999999995 37.0 37.0 37.0 33.0 40.0 54-55 36.286125 37.0 37.0 37.0 33.0 40.0 56-57 36.1265 37.0 37.0 37.0 33.0 40.0 58-59 35.971999999999994 37.0 37.0 37.0 33.0 40.0 60-61 35.847375 37.0 37.0 37.0 33.0 38.5 62-63 35.5985 37.0 35.0 37.0 33.0 37.0 64-65 35.366375000000005 37.0 33.0 37.0 33.0 37.0 66-67 35.275875 37.0 33.0 37.0 33.0 37.0 68-69 34.33725 35.0 33.0 37.0 30.0 37.0 70-71 34.57326965448172 37.0 33.0 37.0 33.0 37.0 72-73 35.13147763556508 37.0 33.0 37.0 33.0 37.0 74-75 35.11384210137676 37.0 33.0 37.0 33.0 37.0 76-77 34.95262461476618 37.0 33.0 37.0 33.0 37.0 78-79 35.11197517125873 37.0 33.0 37.0 33.0 37.0 80-81 34.994433671937884 37.0 33.0 37.0 33.0 37.0 82-83 34.66954689363595 37.0 33.0 37.0 33.0 37.0 84-85 34.769050795553156 37.0 33.0 37.0 33.0 37.0 86-87 34.731919875130075 37.0 33.0 37.0 33.0 37.0 88-89 34.642819979188346 37.0 33.0 37.0 33.0 37.0 90-91 34.24895941727367 37.0 33.0 37.0 33.0 37.0 92-93 34.17377731529657 37.0 33.0 37.0 33.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 3.0 21 6.0 22 6.0 23 9.0 24 8.0 25 12.0 26 17.0 27 17.0 28 26.0 29 43.0 30 59.0 31 82.0 32 74.0 33 110.0 34 200.0 35 371.0 36 878.0 37 1055.0 38 990.0 39 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 82.22500000000001 4.05 4.55 9.175 2 62.025 21.8 11.15 5.025 3 34.150000000000006 36.625 16.55 12.675 4 33.15 26.05 20.3 20.5 5 21.65 33.4 26.224999999999998 18.725 6 20.325 37.875 26.150000000000002 15.65 7 36.275 28.95 19.85 14.924999999999999 8 29.925 30.025000000000002 24.7 15.35 9 27.925 27.500000000000004 26.85 17.724999999999998 10-11 24.8625 28.775000000000002 27.650000000000002 18.712500000000002 12-13 28.4125 25.0625 28.9 17.625 14-15 21.8625 25.8625 32.425 19.85 16-17 24.625 31.85 26.137500000000003 17.3875 18-19 23.9875 26.087500000000002 28.1875 21.7375 20-21 25.074999999999996 25.4625 28.5875 20.875 22-23 26.487500000000004 24.45 28.5875 20.474999999999998 24-25 25.0 24.474999999999998 30.049999999999997 20.474999999999998 26-27 25.224999999999998 25.2875 30.025000000000002 19.4625 28-29 24.65 25.7375 28.7375 20.875 30-31 26.150000000000002 25.2625 27.85 20.7375 32-33 23.599999999999998 25.825 30.025000000000002 20.549999999999997 34-35 25.137500000000003 23.8125 29.1625 21.8875 36-37 24.337500000000002 25.074999999999996 29.9 20.6875 38-39 24.8 25.624999999999996 30.7375 18.8375 40-41 25.4 25.0125 28.6375 20.95 42-43 23.799999999999997 27.6 28.675 19.925 44-45 23.2125 25.45 30.1375 21.2 46-47 25.5375 24.212500000000002 28.575 21.675 48-49 23.7625 25.587500000000002 29.75 20.9 50-51 24.587500000000002 25.837500000000002 29.025000000000002 20.549999999999997 52-53 25.7125 26.200000000000003 27.5875 20.5 54-55 23.8375 27.3625 28.875 19.925 56-57 24.1875 25.775 30.162499999999998 19.875 58-59 23.775 24.375 30.475 21.375 60-61 24.2375 25.687500000000004 29.65 20.424999999999997 62-63 22.4375 27.55 30.7125 19.3 64-65 23.5625 27.0875 29.65 19.7 66-67 24.2375 26.687499999999996 29.4375 19.6375 68-69 23.05 25.912499999999998 31.05 19.9875 70-71 24.55591693770328 25.431573680260193 29.597197898423815 20.41531148361271 72-73 24.450583950772323 25.404998116287832 30.516137134245884 19.628280798693957 74-75 22.83335435852151 25.974517471931375 31.18455910180396 20.007569067743155 76-77 21.93793540215326 25.763141228625713 31.323622545915136 20.97530082330589 78-79 23.864070255822835 25.31500572737686 30.698739977090494 20.12218403970981 80-81 23.647782619841067 26.339400153806714 31.54319405280697 18.469623173545244 82-83 22.840542285345382 25.848934796642997 31.71078114912847 19.59974176888315 84-85 23.119885699441486 24.652552279516822 31.900246785296794 20.3273152357449 86-87 22.90582726326743 25.676378772112386 31.72476586888658 19.69302809573361 88-89 21.709157127991674 27.458376690946928 31.763787721123833 19.068678459937566 90-91 24.167533818938605 26.834027055150884 30.072840790842868 18.925598335067637 92-93 22.736732570239333 27.99167533818939 30.853277835587928 18.41831425598335 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 3.0 18 4.5 19 2.0 20 1.5 21 2.5 22 2.5 23 5.5 24 8.5 25 7.0 26 11.0 27 19.5 28 28.5 29 36.0 30 32.0 31 38.0 32 54.5 33 70.5 34 86.5 35 112.5 36 119.5 37 120.0 38 169.5 39 207.5 40 205.0 41 208.0 42 214.5 43 211.5 44 210.5 45 198.0 46 194.5 47 195.5 48 179.0 49 178.0 50 171.0 51 147.5 52 129.0 53 114.0 54 99.0 55 78.0 56 62.5 57 58.0 58 54.5 59 43.0 60 40.5 61 42.0 62 33.0 63 29.0 64 28.5 65 17.5 66 11.0 67 13.5 68 9.5 69 6.5 70 6.0 71 5.0 72 3.5 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 6.0 71 7.0 72 11.0 73 9.0 74 7.0 75 9.0 76 7.0 77 10.0 78 11.0 79 15.0 80 14.0 81 15.0 82 13.0 83 11.0 84 11.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3844.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.65 #Duplication Level Percentage of deduplicated Percentage of total 1 93.65262550490479 81.15 2 4.010386612810156 6.950000000000001 3 0.8078476630121176 2.1 4 0.5770340450086555 2.0 5 0.2308136180034622 1.0 6 0.17311021350259664 0.8999999999999999 7 0.1154068090017311 0.7000000000000001 8 0.028851702250432775 0.2 9 0.05770340450086555 0.44999999999999996 >10 0.3462204270051933 4.55 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 27 0.675 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 19 0.475 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 18 0.44999999999999996 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 18 0.44999999999999996 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 16 0.4 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 15 0.375 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 15 0.375 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 12 0.3 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 11 0.27499999999999997 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 11 0.27499999999999997 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 10 0.25 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 10 0.25 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 9 0.22499999999999998 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 9 0.22499999999999998 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 8 0.2 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 7 0.17500000000000002 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 7 0.17500000000000002 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 7 0.17500000000000002 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 7 0.17500000000000002 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 6 0.15 No Hit GGCAAGGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAAG 6 0.15 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 6 0.15 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 6 0.15 No Hit TAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTTGACGGCGGACT 6 0.15 No Hit AAATCATATGGTCGTACTGTAAATCTTTTGTGTCTAGTATAATTCTATAT 6 0.15 No Hit GGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTT 5 0.125 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 5 0.125 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 5 0.125 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 5 0.125 No Hit GGGCTCGAGGAGCATATGTACATTTGAACCCTGACTACACATATACACAC 5 0.125 No Hit GAATCAAGTCATCTCATTCTCATCTATGCAATTGCAAACACAACACAGGG 5 0.125 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 5 0.125 No Hit AATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158218 READS because READLEN < 1 Read 158218 spots for ERR6133368.sra Written 158218 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra Rejected 158217 READS because READLEN < 1 Read 158217 spots for ERR6133368.sra Written 158217 spots for ERR6133368.sra SRR ids: ['ERR6133368.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_l7abf7s8 ERR6133368.sra spots: 3164341 blocks: [[1, 158217], [158218, 316434], [316435, 474651], [474652, 632868], [632869, 791085], [791086, 949302], [949303, 1107519], [1107520, 1265736], [1265737, 1423953], [1423954, 1582170], [1582171, 1740387], [1740388, 1898604], [1898605, 2056821], [2056822, 2215038], [2215039, 2373255], [2373256, 2531472], [2531473, 2689689], [2689690, 2847906], [2847907, 3006123], [3006124, 3164341]] ERR6133368 file size 698193 ERR6133368 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133368 ERR6133368_1.fastq Input file: ERR6133368_1.fastq trimmed: ERR6133368-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 01:38:21 2024 >> started Sat Dec 7 01:38:24 2024 >> done (2.473s) 3164341 reads processed; of these: 71 ( 0.00%) short reads filtered out after trimming by size control 5 ( 0.00%) empty reads filtered out after trimming by size control 3164265 (100.00%) reads available; of these: 118865 ( 3.76%) trimmed reads available after processing 3045400 (96.24%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 9 0.00% 20 9 0.00% 21 1 0.00% 22 5 0.00% 23 1 0.00% 24 3 0.00% 25 1 0.00% 26 3 0.00% 27 7 0.00% 28 3 0.00% 29 64 0.00% 30 5 0.00% 31 2 0.00% 32 6 0.00% 33 1 0.00% 34 1 0.00% 35 2 0.00% 36 2 0.00% 37 3 0.00% 38 20 0.00% 39 30 0.00% 40 36 0.00% 41 17 0.00% 42 13 0.00% 43 13 0.00% 44 22 0.00% 45 26 0.00% 46 50 0.00% 47 79 0.00% 48 166 0.01% 49 1043 0.03% 50 275 0.01% 51 305 0.01% 52 287 0.01% 53 371 0.01% 54 431 0.01% 55 495 0.02% 56 515 0.02% 57 675 0.02% 58 570 0.02% 59 681 0.02% 60 863 0.03% 61 680 0.02% 62 704 0.02% 63 949 0.03% 64 950 0.03% 65 945 0.03% 66 1013 0.03% 67 1149 0.04% 68 1375 0.04% 69 730 0.02% 70 9063 0.29% 71 8898 0.28% 72 10401 0.33% 73 9469 0.30% 74 10203 0.32% 75 10737 0.34% 76 9712 0.31% 77 10197 0.32% 78 11027 0.35% 79 11802 0.37% 80 11364 0.36% 81 11393 0.36% 82 13791 0.44% 83 15904 0.50% 84 14061 0.44% 85 4913 0.16% 86 5559 0.18% 87 6328 0.20% 88 8223 0.26% 89 7864 0.25% 90 10795 0.34% 91 11851 0.37% 92 13661 0.43% 93 2911433 92.01% 3164265 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=9.25 fanout-score-rank=20 prefix-density=0.63 prefix-fanout=5.5 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=48.30 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=2.6 sequence=GTCGCCGCCTACCGCGAGCTCATCAATGGTGACCTCGTCGTCGACGACGCCGACATCGGATACTAATTGGCGGGCCGTGCGTGCACGCATGGTTGGCTAGTCGGTCGGTCCCCGGTCAGCTGGGCAATGGGGGATGTGAAGGCTTGTGGTGCCTGTGTGGGCTTGGTGTAGGAGCTGATCGATAGGATAAAGGAGGAATGGAAGCTCTCTTGTATCTAAGAAACTGAAATCTTGTATAACTGTACCTCTCTTGTGTGTATTTTGCCAAGTTCAAATTGTTGAGAACG Started job on | Dec 07 01:38:41 Started mapping on | Dec 07 01:38:41 Finished on | Dec 07 01:38:48 Mapping speed, Million of reads per hour | 1627.34 Number of input reads | 3164265 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 2500865 Uniquely mapped reads % | 79.03% Average mapped length | 91.42 Number of splices: Total | 86311 Number of splices: Annotated (sjdb) | 67844 Number of splices: GT/AG | 79467 Number of splices: GC/AG | 2853 Number of splices: AT/AC | 29 Number of splices: Non-canonical | 3962 Mismatch rate per base, % | 0.58% Deletion rate per base | 0.05% Deletion average length | 1.90 Insertion rate per base | 0.02% Insertion average length | 1.73 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 543267 % of reads mapped to multiple loci | 17.17% Number of reads mapped to too many loci | 25442 % of reads mapped to too many loci | 0.80% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.89% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 120133 120133 120133 N_multimapping 543267 543267 543267 N_noFeature 133516 158519 2379103 N_ambiguous 105109 8375 325 UnstrandedReadsAssigned:2262240 PositiveStrandReadsAssigned:2333971 NegativeStrandReadsAssigned:121437 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133368 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133368-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,164,265 reads, 2,648,351 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,011 rounds 52973 ERR6133368.ke.tsv 35125 ERR6133368.se.tsv 88098 total ==> ERR6133368.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 65 25.0024 PNS24243 293 194 0 0 KQK14069 1603 1504 167.079 58.6268 KQK14071 474 375 0 0 ==> ERR6133368.se.tsv <== BRADI_1g14170v3 174 BRADI_1g53295v3 42 BRADI_1g59795v3 34 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 37 BRADI_1g74790v3 22 BRADI_1g09890v3 0 BRADI_1g77505v3 51 BRADI_1g48960v3 0 ERR6133368 completed mapping pipeline successfully