Starting /dee2/code/volunteer_pipeline.sh ERR6133370 current disk space = 1547990487040 free memory = 1599212592 ERR6133370 SRAfilesize e9346c524f8ce8624df3e81b691e621b ERR6133370.sra ERR6133370.sra file validated ERR6133370 is single end ERR6133370 is conventional basespace ERR6133370 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133370_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.9085 37.0 37.0 37.0 37.0 37.0 2 36.80925 37.0 37.0 37.0 37.0 37.0 3 36.61975 37.0 37.0 37.0 37.0 37.0 4 36.03425 37.0 37.0 37.0 33.0 37.0 5 35.983 37.0 37.0 37.0 33.0 37.0 6 36.1765 37.0 37.0 37.0 33.0 37.0 7 38.1485 40.0 37.0 40.0 37.0 40.0 8 38.17225 40.0 37.0 40.0 37.0 40.0 9 38.0795 40.0 37.0 40.0 37.0 40.0 10-11 38.06075 40.0 37.0 40.0 37.0 40.0 12-13 38.135125 40.0 37.0 40.0 37.0 40.0 14-15 38.037499999999994 40.0 37.0 40.0 35.0 40.0 16-17 37.99125 40.0 37.0 40.0 35.0 40.0 18-19 37.89175 40.0 37.0 40.0 33.0 40.0 20-21 37.723625 40.0 37.0 40.0 33.0 40.0 22-23 37.68875 40.0 37.0 40.0 33.0 40.0 24-25 37.5865 37.0 37.0 40.0 33.0 40.0 26-27 37.3815 37.0 37.0 40.0 33.0 40.0 28-29 37.37975 37.0 37.0 40.0 33.0 40.0 30-31 37.228125 37.0 37.0 40.0 33.0 40.0 32-33 36.962999999999994 37.0 37.0 40.0 33.0 40.0 34-35 36.68125 37.0 37.0 40.0 33.0 40.0 36-37 36.758250000000004 37.0 37.0 40.0 33.0 40.0 38-39 36.893125 37.0 37.0 40.0 33.0 40.0 40-41 37.094375 37.0 37.0 40.0 33.0 40.0 42-43 36.9785 37.0 37.0 40.0 33.0 40.0 44-45 36.89175 37.0 37.0 40.0 33.0 40.0 46-47 36.687625 37.0 37.0 40.0 33.0 40.0 48-49 36.49225 37.0 37.0 40.0 33.0 40.0 50-51 36.445499999999996 37.0 37.0 37.0 33.0 40.0 52-53 36.294375 37.0 37.0 37.0 33.0 40.0 54-55 36.130250000000004 37.0 37.0 37.0 33.0 40.0 56-57 35.933 37.0 37.0 37.0 33.0 40.0 58-59 35.698625 37.0 37.0 37.0 33.0 38.5 60-61 35.64275 37.0 35.0 37.0 33.0 37.0 62-63 35.37875 37.0 33.0 37.0 33.0 37.0 64-65 35.148250000000004 37.0 33.0 37.0 33.0 37.0 66-67 35.167 37.0 33.0 37.0 33.0 37.0 68-69 34.237375 35.0 33.0 37.0 30.0 37.0 70-71 34.32790758145363 37.0 33.0 37.0 30.0 37.0 72-73 34.73933075865606 37.0 33.0 37.0 33.0 37.0 74-75 34.791391768310575 37.0 33.0 37.0 33.0 37.0 76-77 34.780072387341306 37.0 33.0 37.0 33.0 37.0 78-79 34.85922914117599 37.0 33.0 37.0 33.0 37.0 80-81 34.615688104863025 37.0 33.0 37.0 33.0 37.0 82-83 34.29448160145417 37.0 33.0 37.0 30.0 37.0 84-85 34.404299422606144 37.0 33.0 37.0 33.0 37.0 86-87 34.40012886597938 37.0 33.0 37.0 33.0 37.0 88-89 34.35451030927835 37.0 33.0 37.0 33.0 37.0 90-91 33.840721649484536 37.0 33.0 37.0 27.0 37.0 92-93 33.8319587628866 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 7.0 22 12.0 23 8.0 24 13.0 25 18.0 26 19.0 27 26.0 28 33.0 29 35.0 30 66.0 31 78.0 32 83.0 33 143.0 34 209.0 35 396.0 36 906.0 37 1052.0 38 870.0 39 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 83.45 3.5249999999999995 4.6 8.425 2 61.8 22.15 10.4 5.65 3 35.725 35.25 15.4 13.625000000000002 4 34.699999999999996 25.1 18.3 21.9 5 22.95 32.7 26.200000000000003 18.15 6 19.625 37.65 26.474999999999998 16.25 7 35.875 29.175 19.1 15.85 8 30.725 28.925 23.3 17.05 9 26.325 26.924999999999997 26.775 19.975 10-11 25.25 27.5875 27.450000000000003 19.7125 12-13 29.475 24.7875 26.437500000000004 19.3 14-15 23.575 26.400000000000002 29.75 20.275000000000002 16-17 25.424999999999997 31.937500000000004 24.587500000000002 18.05 18-19 24.825 26.6125 25.5125 23.05 20-21 25.5375 26.150000000000002 26.5375 21.775 22-23 27.0 23.200000000000003 27.3125 22.4875 24-25 26.974999999999998 23.575 28.299999999999997 21.15 26-27 25.587500000000002 25.674999999999997 28.5875 20.150000000000002 28-29 26.1125 26.737499999999997 25.7375 21.4125 30-31 28.225 24.9375 25.55 21.2875 32-33 24.6625 27.037499999999998 27.2625 21.0375 34-35 26.187500000000004 23.9 27.375 22.537499999999998 36-37 25.5625 22.925 28.325 23.1875 38-39 24.762500000000003 25.2375 29.762499999999996 20.2375 40-41 26.724999999999998 26.05 26.3125 20.9125 42-43 25.0125 28.1875 26.55 20.25 44-45 23.8125 24.9 28.537499999999998 22.75 46-47 24.925 24.0625 27.025 23.9875 48-49 25.224999999999998 24.8125 29.6875 20.275000000000002 50-51 25.4625 25.374999999999996 28.475 20.6875 52-53 26.275 26.337500000000002 26.3 21.087500000000002 54-55 23.849999999999998 27.675 27.1125 21.3625 56-57 25.9625 25.9625 27.1625 20.9125 58-59 24.224999999999998 24.15 29.262500000000003 22.3625 60-61 25.7375 23.525 29.062500000000004 21.675 62-63 22.4625 27.650000000000002 30.312499999999996 19.575 64-65 24.925 24.925 28.6875 21.462500000000002 66-67 25.825 27.025 27.150000000000002 20.0 68-69 25.025 25.8 27.0 22.175 70-71 24.943679599499376 25.06883604505632 27.684605757196497 22.302878598247812 72-73 26.401105805478764 24.7423975873335 27.544609198291027 21.311887408896705 74-75 23.27607981813589 27.241727709017425 28.82040919424097 20.661783278605707 76-77 23.374318671568005 25.922170110280135 28.533400938014957 22.1701102801369 78-79 25.375413591244588 25.031814711122426 28.658691779078644 20.934079918554342 80-81 25.162856048026566 26.23579001149572 28.969217013667137 19.632136926810574 82-83 24.695317511225145 24.105195638229635 29.531751122514432 21.66773572803079 84-85 23.612720484099395 24.578344277069654 29.75408780739024 22.05484743144071 86-87 23.56958762886598 25.592783505154635 30.721649484536083 20.1159793814433 88-89 22.24226804123711 28.4020618556701 29.34278350515464 20.012886597938145 90-91 25.502577319587626 26.25 28.543814432989688 19.70360824742268 92-93 24.484536082474225 26.713917525773194 28.595360824742265 20.20618556701031 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 3.5 18 4.5 19 2.0 20 1.0 21 1.0 22 0.5 23 1.5 24 3.5 25 3.0 26 5.5 27 11.0 28 16.5 29 19.5 30 18.5 31 25.0 32 37.5 33 54.5 34 62.5 35 74.5 36 91.5 37 118.0 38 150.0 39 164.5 40 172.5 41 176.5 42 177.0 43 174.5 44 176.5 45 169.0 46 193.5 47 217.5 48 196.0 49 187.5 50 204.0 51 189.5 52 147.0 53 156.5 54 151.0 55 98.5 56 74.0 57 72.0 58 72.0 59 60.5 60 53.0 61 53.0 62 45.0 63 41.0 64 37.0 65 30.5 66 27.0 67 20.5 68 15.0 69 12.5 70 6.5 71 3.0 72 3.0 73 5.5 74 5.0 75 2.0 76 1.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 10.0 71 5.0 72 12.0 73 11.0 74 6.0 75 9.0 76 5.0 77 8.0 78 10.0 79 7.0 80 5.0 81 11.0 82 7.0 83 7.0 84 7.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3880.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.85000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 93.6195587358378 78.5 2 3.9952295766249257 6.7 3 0.8348240906380441 2.1 4 0.5664877757901015 1.9 5 0.20870602265951102 0.8750000000000001 6 0.1490757304710793 0.75 7 0.08944543828264759 0.525 8 0.08944543828264759 0.6 9 0.0 0.0 >10 0.41741204531902204 6.7 >50 0.02981514609421586 1.35 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 54 1.35 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 36 0.8999999999999999 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 31 0.775 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 27 0.675 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 25 0.625 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 23 0.575 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 20 0.5 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 17 0.42500000000000004 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 16 0.4 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 16 0.4 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 13 0.325 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 13 0.325 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 11 0.27499999999999997 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 10 0.25 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 10 0.25 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 8 0.2 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 8 0.2 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 8 0.2 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 7 0.17500000000000002 No Hit TAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTTGACGGCGGACT 7 0.17500000000000002 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 7 0.17500000000000002 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 6 0.15 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 6 0.15 No Hit AGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATGAGCCT 6 0.15 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 6 0.15 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 6 0.15 No Hit GGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTC 5 0.125 No Hit GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC 5 0.125 No Hit GGGCAAGGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAA 5 0.125 No Hit GTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCA 5 0.125 No Hit GGACTGGGTATCCATGCCAGGTGTTATACCAGTAGCTTCAGGTGGTATTC 5 0.125 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 5 0.125 No Hit TCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATCT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Rejected 195258 READS because READLEN < 1 Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195262 READS because READLEN < 1 Read 195262 spots for ERR6133370.sra Written 195262 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra Rejected 195258 READS because READLEN < 1 Read 195258 spots for ERR6133370.sra Written 195258 spots for ERR6133370.sra SRR ids: ['ERR6133370.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ryz126xk ERR6133370.sra spots: 3905164 blocks: [[1, 195258], [195259, 390516], [390517, 585774], [585775, 781032], [781033, 976290], [976291, 1171548], [1171549, 1366806], [1366807, 1562064], [1562065, 1757322], [1757323, 1952580], [1952581, 2147838], [2147839, 2343096], [2343097, 2538354], [2538355, 2733612], [2733613, 2928870], [2928871, 3124128], [3124129, 3319386], [3319387, 3514644], [3514645, 3709902], [3709903, 3905164]] ERR6133370 file size 863625 ERR6133370 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133370 ERR6133370_1.fastq Input file: ERR6133370_1.fastq trimmed: ERR6133370-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 01:50:22 2024 >> started Sat Dec 7 01:50:24 2024 >> done (2.255s) 3905164 reads processed; of these: 63 ( 0.00%) short reads filtered out after trimming by size control 3 ( 0.00%) empty reads filtered out after trimming by size control 3905098 (100.00%) reads available; of these: 160773 ( 4.12%) trimmed reads available after processing 3744325 (95.88%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 8 0.00% 20 5 0.00% 21 8 0.00% 22 5 0.00% 23 4 0.00% 24 5 0.00% 25 3 0.00% 26 5 0.00% 27 4 0.00% 28 5 0.00% 29 50 0.00% 30 5 0.00% 31 1 0.00% 32 6 0.00% 33 7 0.00% 34 4 0.00% 35 3 0.00% 36 8 0.00% 37 8 0.00% 38 13 0.00% 39 21 0.00% 40 44 0.00% 41 18 0.00% 42 20 0.00% 43 17 0.00% 44 17 0.00% 45 42 0.00% 46 74 0.00% 47 88 0.00% 48 189 0.00% 49 1263 0.03% 50 351 0.01% 51 354 0.01% 52 359 0.01% 53 425 0.01% 54 516 0.01% 55 594 0.02% 56 680 0.02% 57 750 0.02% 58 785 0.02% 59 913 0.02% 60 1116 0.03% 61 956 0.02% 62 1031 0.03% 63 1442 0.04% 64 1210 0.03% 65 1324 0.03% 66 1497 0.04% 67 1614 0.04% 68 2156 0.06% 69 921 0.02% 70 8355 0.21% 71 8275 0.21% 72 10294 0.26% 73 9267 0.24% 74 9760 0.25% 75 9994 0.26% 76 8856 0.23% 77 9450 0.24% 78 10737 0.27% 79 11824 0.30% 80 11217 0.29% 81 11009 0.28% 82 13057 0.33% 83 16961 0.43% 84 13873 0.36% 85 6540 0.17% 86 7281 0.19% 87 8661 0.22% 88 10990 0.28% 89 10734 0.27% 90 14931 0.38% 91 17426 0.45% 92 18479 0.47% 93 3626180 92.86% 3905098 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=7.18 fanout-score-rank=19 prefix-density=0.67 prefix-fanout=2.9 sequence=GCCGCCGCCGCCAAGGAAGGCATGTTCGTCAAGAACTACAGCTACTGATCCTAATCGCATCAAGCTTCAACGCCTGTGAGTGAAAACCAGTGATGAGAGTGCTGCTGCTAGCTAGCGCCGGCATTGATGAGCTTGAGAGGGCACTGTAGCCAGTGTGTCAGTCGTTGTTAAATTACAGGTTGAGATCATCAGCGTACTCCGATGGGAGGTGGACATCAGAAAGTATACTGTGTTTTACCACCCTAATTAAGTAAACAACTTTTGGAATGGTGATCATCTTACTGTTTTAAATAAATCTGTTTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=72.53 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=4.6 sequence=AGTTTGTTGACAAGTACGGCGCCAACGTCGACGGCTACAGCCCGATCTACACGCCGGAGGTATGGTCCGAATCTGGCGACCGCTACGCCGGTGGGACGACGGGGCTCCTGATCTGGGCCGTCACCCTGGCCGGCCTCCTCGGCGGCGGCGCCCTCCTCGTCTACAACACCAGCGCTCTCGCCGGCTAATTAAGAATCAAGTCATCTCATTCTCATCTATGCAATTGCAAACACAACACAGGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTACCTGCTGTGAAATGTACGTATGTGATCGATGATGCCAAGTACTTGATCGAAACGCATCGCTTAATTTTATGTATGTATAACACTTGCTACTACGTACACACTTCCTACTTTGGGATTATC Started job on | Dec 07 01:50:37 Started mapping on | Dec 07 01:50:37 Finished on | Dec 07 01:50:45 Mapping speed, Million of reads per hour | 1757.29 Number of input reads | 3905098 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 2911613 Uniquely mapped reads % | 74.56% Average mapped length | 91.74 Number of splices: Total | 190466 Number of splices: Annotated (sjdb) | 162323 Number of splices: GT/AG | 183071 Number of splices: GC/AG | 3775 Number of splices: AT/AC | 40 Number of splices: Non-canonical | 3580 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.03% Deletion average length | 1.76 Insertion rate per base | 0.02% Insertion average length | 2.05 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 872737 % of reads mapped to multiple loci | 22.35% Number of reads mapped to too many loci | 29912 % of reads mapped to too many loci | 0.77% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.23% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 120748 120748 120748 N_multimapping 872737 872737 872737 N_noFeature 134623 164836 2778494 N_ambiguous 111875 9038 266 UnstrandedReadsAssigned:2665115 PositiveStrandReadsAssigned:2737739 NegativeStrandReadsAssigned:132853 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133370 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133370-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,905,098 reads, 3,332,153 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,133 rounds 52973 ERR6133370.ke.tsv 35125 ERR6133370.se.tsv 88098 total ==> ERR6133370.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 76 22.6965 PNS24243 293 194 0 0 KQK14069 1603 1504 67 18.2527 KQK14071 474 375 0 0 ==> ERR6133370.se.tsv <== BRADI_1g14170v3 67 BRADI_1g53295v3 97 BRADI_1g59795v3 21 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 26 BRADI_1g74790v3 29 BRADI_1g09890v3 0 BRADI_1g77505v3 66 BRADI_1g48960v3 0 ERR6133370 completed mapping pipeline successfully