Starting /dee2/code/volunteer_pipeline.sh ERR6133373 current disk space = 1547911213056 free memory = 1444319544 ERR6133373 SRAfilesize 798bbd2031b1e3ef8203b8dfbd87c05c ERR6133373.sra ERR6133373.sra file validated ERR6133373 is single end ERR6133373 is conventional basespace ERR6133373 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133373_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.88275 37.0 37.0 37.0 37.0 37.0 2 36.78525 37.0 37.0 37.0 37.0 37.0 3 36.67275 37.0 37.0 37.0 37.0 37.0 4 36.18175 37.0 37.0 37.0 33.0 37.0 5 36.135 37.0 37.0 37.0 33.0 37.0 6 36.2835 37.0 37.0 37.0 37.0 37.0 7 38.301 40.0 37.0 40.0 37.0 40.0 8 38.341 40.0 37.0 40.0 37.0 40.0 9 38.28925 40.0 37.0 40.0 37.0 40.0 10-11 38.241 40.0 37.0 40.0 37.0 40.0 12-13 38.277 40.0 37.0 40.0 37.0 40.0 14-15 38.211625 40.0 37.0 40.0 37.0 40.0 16-17 38.160375 40.0 37.0 40.0 37.0 40.0 18-19 38.016999999999996 40.0 37.0 40.0 33.0 40.0 20-21 37.956374999999994 40.0 37.0 40.0 33.0 40.0 22-23 37.920249999999996 40.0 37.0 40.0 33.0 40.0 24-25 37.736125 40.0 37.0 40.0 33.0 40.0 26-27 37.624375 40.0 37.0 40.0 33.0 40.0 28-29 37.552375 40.0 37.0 40.0 33.0 40.0 30-31 37.462374999999994 37.0 37.0 40.0 33.0 40.0 32-33 37.190875 37.0 37.0 40.0 33.0 40.0 34-35 37.005875 37.0 37.0 40.0 33.0 40.0 36-37 36.959625 37.0 37.0 40.0 33.0 40.0 38-39 37.084 37.0 37.0 40.0 33.0 40.0 40-41 37.230374999999995 37.0 37.0 40.0 33.0 40.0 42-43 37.17525 37.0 37.0 40.0 33.0 40.0 44-45 37.1595 37.0 37.0 40.0 33.0 40.0 46-47 36.7985 37.0 37.0 40.0 33.0 40.0 48-49 36.779375 37.0 37.0 40.0 33.0 40.0 50-51 36.640625 37.0 37.0 40.0 33.0 40.0 52-53 36.44262500000001 37.0 37.0 37.0 33.0 40.0 54-55 36.258625 37.0 37.0 37.0 33.0 40.0 56-57 36.051625 37.0 37.0 37.0 33.0 40.0 58-59 35.895875 37.0 37.0 37.0 33.0 40.0 60-61 35.802125000000004 37.0 37.0 37.0 33.0 38.5 62-63 35.542625 37.0 35.0 37.0 33.0 37.0 64-65 35.242125 37.0 33.0 37.0 33.0 37.0 66-67 35.212625 37.0 33.0 37.0 33.0 37.0 68-69 34.208375000000004 35.0 33.0 37.0 30.0 37.0 70-71 34.4956137359199 37.0 33.0 37.0 33.0 37.0 72-73 34.968856616896616 37.0 33.0 37.0 33.0 37.0 74-75 34.913767088535565 37.0 33.0 37.0 33.0 37.0 76-77 34.7438954329853 37.0 33.0 37.0 33.0 37.0 78-79 34.90262828731355 37.0 33.0 37.0 33.0 37.0 80-81 34.82502072220406 37.0 33.0 37.0 33.0 37.0 82-83 34.43182467175414 37.0 33.0 37.0 33.0 37.0 84-85 34.531454901684654 37.0 33.0 37.0 33.0 37.0 86-87 34.49771225216065 37.0 33.0 37.0 33.0 37.0 88-89 34.462252160650735 37.0 33.0 37.0 33.0 37.0 90-91 34.13472292831723 37.0 33.0 37.0 30.0 37.0 92-93 33.966319267920696 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 3.0 21 8.0 22 8.0 23 12.0 24 15.0 25 13.0 26 17.0 27 19.0 28 26.0 29 35.0 30 55.0 31 70.0 32 87.0 33 132.0 34 180.0 35 383.0 36 866.0 37 1080.0 38 967.0 39 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 79.875 6.75 5.45 7.925 2 56.55 24.125 13.05 6.275 3 32.2 35.375 18.15 14.274999999999999 4 33.1 26.75 19.3 20.849999999999998 5 23.825 30.75 26.400000000000002 19.025 6 20.525 37.625 25.275 16.575 7 35.35 28.425 21.375 14.85 8 30.725 28.825 22.25 18.2 9 25.424999999999997 27.075 28.025 19.475 10-11 25.7125 27.725 27.325 19.2375 12-13 28.9375 24.9 27.800000000000004 18.3625 14-15 22.725 27.325 30.587500000000002 19.3625 16-17 25.2625 31.4375 24.837500000000002 18.462500000000002 18-19 23.9875 27.212500000000002 26.200000000000003 22.6 20-21 25.924999999999997 26.5 27.275 20.3 22-23 27.3 23.2625 28.575 20.8625 24-25 26.625 24.0125 28.125 21.2375 26-27 25.2875 26.2125 28.5625 19.9375 28-29 25.724999999999998 26.0125 26.787499999999998 21.475 30-31 28.037499999999998 25.324999999999996 26.8 19.8375 32-33 24.6875 28.487499999999997 26.75 20.075000000000003 34-35 24.7 25.525 28.299999999999997 21.475 36-37 25.0125 24.175 27.275 23.5375 38-39 26.787499999999998 25.412499999999998 28.8875 18.912499999999998 40-41 27.075 23.7375 27.6625 21.525 42-43 24.725 28.6875 26.25 20.3375 44-45 24.099999999999998 24.85 29.1875 21.8625 46-47 24.65 23.2875 28.425 23.6375 48-49 25.674999999999997 24.8125 28.3125 21.2 50-51 25.2125 25.6125 28.4125 20.7625 52-53 26.674999999999997 25.074999999999996 26.787499999999998 21.462500000000002 54-55 24.175 26.724999999999998 27.775 21.325 56-57 25.674999999999997 25.900000000000002 27.625 20.8 58-59 23.225 24.887500000000003 28.875 23.0125 60-61 25.95 24.075 29.549999999999997 20.424999999999997 62-63 22.7625 27.0625 30.112499999999997 20.0625 64-65 24.9 26.387500000000003 28.1625 20.549999999999997 66-67 25.662499999999998 27.6625 27.6375 19.037499999999998 68-69 24.474999999999998 25.900000000000002 27.8875 21.7375 70-71 24.90306441525954 25.57848655409631 28.042526579111943 21.47592245153221 72-73 26.873903233893202 25.36976685886187 27.688643770368515 20.06768613687641 74-75 23.43474981141564 27.244153884837818 28.400804626602966 20.920291677143577 76-77 22.877299067775258 25.951121189216426 28.621819098009578 22.54976064499874 78-79 25.35051155740811 24.84526967285588 29.05140836175319 20.75281040798282 80-81 23.553980508796354 27.768636881407417 29.160865713200863 19.516516896595366 82-83 25.30135769572389 23.448800913589647 30.92247176754219 20.32736962314427 84-85 23.764137755750415 24.005591561824883 30.486720040665904 21.743550641758798 86-87 22.35638027452974 26.677681748856124 30.439755973563802 20.52618200305033 88-89 21.97508896797153 29.067107269954246 29.295882053889173 19.661921708185055 90-91 25.31774275546518 26.56329435688866 29.016268429079815 19.102694458566344 92-93 23.690899847483475 27.872394509405186 28.10116929334011 20.335536349771225 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 15.0 18 16.5 19 3.0 20 3.0 21 3.0 22 2.5 23 2.0 24 1.5 25 3.5 26 7.5 27 13.5 28 18.0 29 19.0 30 22.0 31 25.5 32 29.5 33 42.5 34 65.5 35 79.5 36 94.5 37 116.5 38 140.0 39 149.5 40 157.0 41 169.0 42 180.0 43 193.0 44 204.5 45 198.5 46 235.0 47 242.0 48 193.0 49 198.0 50 194.5 51 187.5 52 156.0 53 130.0 54 125.5 55 95.5 56 67.5 57 56.5 58 63.0 59 62.5 60 51.0 61 40.5 62 34.5 63 42.0 64 39.0 65 23.0 66 18.0 67 16.5 68 13.5 69 10.5 70 6.0 71 3.0 72 4.0 73 3.5 74 1.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 5.0 71 3.0 72 6.0 73 7.0 74 4.0 75 5.0 76 2.0 77 6.0 78 7.0 79 3.0 80 3.0 81 7.0 82 3.0 83 4.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3934.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.375 #Duplication Level Percentage of deduplicated Percentage of total 1 94.23368740515933 77.625 2 3.0349013657056148 5.0 3 1.2139605462822458 3.0 4 0.4552352048558422 1.5 5 0.3338391502276176 1.375 6 0.06069802731411229 0.3 7 0.09104704097116845 0.525 8 0.030349013657056147 0.2 9 0.06069802731411229 0.44999999999999996 >10 0.48558421851289835 10.025 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 48 1.2 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 46 1.15 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 39 0.975 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 36 0.8999999999999999 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 30 0.75 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 30 0.75 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 24 0.6 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 22 0.5499999999999999 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 21 0.525 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 20 0.5 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 18 0.44999999999999996 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 16 0.4 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 14 0.35000000000000003 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 14 0.35000000000000003 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 12 0.3 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 11 0.27499999999999997 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 9 0.22499999999999998 No Hit GCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGA 9 0.22499999999999998 No Hit GGGACGCATGCAACGACCATCTACATATAGCTACTCGATCTACCGCTACC 8 0.2 No Hit AGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACG 7 0.17500000000000002 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 7 0.17500000000000002 No Hit GTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTA 7 0.17500000000000002 No Hit CATTACTGATGGAGTGATGGTCCATAGAGCATTAGTTTCACTACCTTCGC 6 0.15 No Hit AAATCATATGGTCGTACTGTAAATCTTTTGTGTCTAGTATAATTCTATAT 6 0.15 No Hit GAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGG 5 0.125 No Hit GTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAG 5 0.125 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 5 0.125 No Hit TGAGTATGATGAGTCTGGTCCAGCGATTGTTCACAGGAAGTGCTTCTAAG 5 0.125 No Hit GGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTC 5 0.125 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 5 0.125 No Hit GTGAGCATATATATTTATACGACGAATAAAAGTCTGCCACGTGGCGGGTA 5 0.125 No Hit GACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTA 5 0.125 No Hit AACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGC 5 0.125 No Hit GGGGAAGGGCGCGCTGGCGCAGCTCAACATCGAGACCGGCGTGCCCATCA 5 0.125 No Hit GGAACTCTACTCTCTGGTCACCGTTGCTGAGATCCCCCAGGAAGGTCTGA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150782 READS because READLEN < 1 Read 150782 spots for ERR6133373.sra Written 150782 spots for ERR6133373.sra Rejected 150797 READS because READLEN < 1 Read 150797 spots for ERR6133373.sra Written 150797 spots for ERR6133373.sra SRR ids: ['ERR6133373.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_s5gldti6 ERR6133373.sra spots: 3015655 blocks: [[1, 150782], [150783, 301564], [301565, 452346], [452347, 603128], [603129, 753910], [753911, 904692], [904693, 1055474], [1055475, 1206256], [1206257, 1357038], [1357039, 1507820], [1507821, 1658602], [1658603, 1809384], [1809385, 1960166], [1960167, 2110948], [2110949, 2261730], [2261731, 2412512], [2412513, 2563294], [2563295, 2714076], [2714077, 2864858], [2864859, 3015655]] ERR6133373 file size 667361 ERR6133373 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133373 ERR6133373_1.fastq Input file: ERR6133373_1.fastq trimmed: ERR6133373-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:00:12 2024 >> started Sat Dec 7 02:00:14 2024 >> done (1.945s) 3015655 reads processed; of these: 81 ( 0.00%) short reads filtered out after trimming by size control 4 ( 0.00%) empty reads filtered out after trimming by size control 3015570 (100.00%) reads available; of these: 125126 ( 4.15%) trimmed reads available after processing 2890444 (95.85%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 3 0.00% 20 5 0.00% 21 5 0.00% 22 4 0.00% 23 5 0.00% 24 4 0.00% 25 5 0.00% 26 2 0.00% 27 5 0.00% 28 4 0.00% 29 17 0.00% 30 1 0.00% 31 1 0.00% 32 3 0.00% 33 2 0.00% 34 4 0.00% 35 5 0.00% 36 3 0.00% 37 6 0.00% 38 5 0.00% 39 4 0.00% 40 5 0.00% 41 7 0.00% 42 10 0.00% 43 9 0.00% 44 8 0.00% 45 22 0.00% 46 38 0.00% 47 86 0.00% 48 214 0.01% 49 1006 0.03% 50 283 0.01% 51 283 0.01% 52 283 0.01% 53 335 0.01% 54 417 0.01% 55 519 0.02% 56 530 0.02% 57 746 0.02% 58 595 0.02% 59 669 0.02% 60 934 0.03% 61 705 0.02% 62 816 0.03% 63 1047 0.03% 64 970 0.03% 65 1003 0.03% 66 1129 0.04% 67 1228 0.04% 68 1473 0.05% 69 701 0.02% 70 4161 0.14% 71 4276 0.14% 72 5501 0.18% 73 4813 0.16% 74 5187 0.17% 75 5713 0.19% 76 5219 0.17% 77 5788 0.19% 78 6560 0.22% 79 7312 0.24% 80 7127 0.24% 81 7873 0.26% 82 8354 0.28% 83 10522 0.35% 84 9087 0.30% 85 5150 0.17% 86 5949 0.20% 87 6897 0.23% 88 8481 0.28% 89 8622 0.29% 90 11535 0.38% 91 13181 0.44% 92 14018 0.46% 93 2828078 93.78% 3015570 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=5.43 fanout-score-rank=31 prefix-density=0.65 prefix-fanout=3.9 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=372.27 fanout-score-rank=1 prefix-density=0.70 prefix-fanout=8.5 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTGCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAATAGATACAGGGTCTCAAACCTTGCTATAGAGTTTTTGCTT Started job on | Dec 07 02:00:31 Started mapping on | Dec 07 02:00:31 Finished on | Dec 07 02:00:38 Mapping speed, Million of reads per hour | 1550.86 Number of input reads | 3015570 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 2232681 Uniquely mapped reads % | 74.04% Average mapped length | 91.86 Number of splices: Total | 168968 Number of splices: Annotated (sjdb) | 142901 Number of splices: GT/AG | 159177 Number of splices: GC/AG | 3582 Number of splices: AT/AC | 55 Number of splices: Non-canonical | 6154 Mismatch rate per base, % | 0.48% Deletion rate per base | 0.03% Deletion average length | 1.79 Insertion rate per base | 0.02% Insertion average length | 1.90 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 684711 % of reads mapped to multiple loci | 22.71% Number of reads mapped to too many loci | 13369 % of reads mapped to too many loci | 0.44% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.74% % of reads unmapped: other | 0.07% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 98178 98178 98178 N_multimapping 684711 684711 684711 N_noFeature 104693 128468 2129228 N_ambiguous 87145 7452 228 UnstrandedReadsAssigned:2040843 PositiveStrandReadsAssigned:2096761 NegativeStrandReadsAssigned:103225 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133373 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133373-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,015,570 reads, 2,552,270 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,009 rounds 52973 ERR6133373.ke.tsv 35125 ERR6133373.se.tsv 88098 total ==> ERR6133373.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 61 23.3853 PNS24243 293 194 0 0 KQK14069 1603 1504 44 15.3876 KQK14071 474 375 1 1.4026 ==> ERR6133373.se.tsv <== BRADI_1g14170v3 45 BRADI_1g53295v3 69 BRADI_1g59795v3 19 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 49 BRADI_1g74790v3 19 BRADI_1g09890v3 0 BRADI_1g77505v3 85 BRADI_1g48960v3 0 ERR6133373 completed mapping pipeline successfully