Starting /dee2/code/volunteer_pipeline.sh ERR6133375 current disk space = 1547923927040 free memory = 1603441396 ERR6133375 SRAfilesize 86738b782a6f90257cfd9b8e967c1525 ERR6133375.sra ERR6133375.sra file validated ERR6133375 is single end ERR6133375 is conventional basespace ERR6133375 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133375_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.9125 37.0 37.0 37.0 37.0 37.0 2 36.77 37.0 37.0 37.0 37.0 37.0 3 36.58625 37.0 37.0 37.0 37.0 37.0 4 36.23725 37.0 37.0 37.0 37.0 37.0 5 36.251 37.0 37.0 37.0 33.0 37.0 6 36.23 37.0 37.0 37.0 33.0 37.0 7 38.32425 40.0 37.0 40.0 37.0 40.0 8 38.29625 40.0 37.0 40.0 37.0 40.0 9 38.366 40.0 37.0 40.0 37.0 40.0 10-11 38.289 40.0 37.0 40.0 37.0 40.0 12-13 38.288 40.0 37.0 40.0 37.0 40.0 14-15 38.1765 40.0 37.0 40.0 37.0 40.0 16-17 38.1345 40.0 37.0 40.0 35.0 40.0 18-19 38.039875 40.0 37.0 40.0 33.0 40.0 20-21 37.942375 40.0 37.0 40.0 33.0 40.0 22-23 37.92275 40.0 37.0 40.0 33.0 40.0 24-25 37.82325 40.0 37.0 40.0 33.0 40.0 26-27 37.678625 40.0 37.0 40.0 33.0 40.0 28-29 37.525375 38.5 37.0 40.0 33.0 40.0 30-31 37.3815 37.0 37.0 40.0 33.0 40.0 32-33 37.132875 37.0 37.0 40.0 33.0 40.0 34-35 36.9715 37.0 37.0 40.0 33.0 40.0 36-37 36.98225 37.0 37.0 40.0 33.0 40.0 38-39 37.045625 37.0 37.0 40.0 33.0 40.0 40-41 37.26375 37.0 37.0 40.0 33.0 40.0 42-43 37.1655 37.0 37.0 40.0 33.0 40.0 44-45 37.116749999999996 37.0 37.0 40.0 33.0 40.0 46-47 36.819625 37.0 37.0 40.0 33.0 40.0 48-49 36.72025 37.0 37.0 40.0 33.0 40.0 50-51 36.60575 37.0 37.0 40.0 33.0 40.0 52-53 36.392125 37.0 37.0 37.0 33.0 40.0 54-55 36.1915 37.0 37.0 37.0 33.0 40.0 56-57 35.930875 37.0 37.0 37.0 33.0 40.0 58-59 35.871375 37.0 37.0 37.0 33.0 40.0 60-61 35.736999999999995 37.0 37.0 37.0 33.0 38.5 62-63 35.414249999999996 37.0 33.0 37.0 33.0 37.0 64-65 35.223875 37.0 33.0 37.0 33.0 37.0 66-67 35.179 37.0 33.0 37.0 33.0 37.0 68-69 34.23425 35.0 33.0 37.0 30.0 37.0 70-71 34.35875982724086 37.0 33.0 37.0 33.0 37.0 72-73 34.93240571757984 37.0 33.0 37.0 33.0 37.0 74-75 34.88575900308307 37.0 33.0 37.0 33.0 37.0 76-77 34.737523188300955 37.0 33.0 37.0 33.0 37.0 78-79 34.87512671065807 37.0 33.0 37.0 33.0 37.0 80-81 34.77437305712175 37.0 33.0 37.0 33.0 37.0 82-83 34.474635030836446 37.0 33.0 37.0 33.0 37.0 84-85 34.4817392232181 37.0 33.0 37.0 33.0 37.0 86-87 34.38923076923077 37.0 33.0 37.0 33.0 37.0 88-89 34.317564102564106 37.0 33.0 37.0 33.0 37.0 90-91 33.95320512820513 37.0 33.0 37.0 27.0 37.0 92-93 33.858974358974365 37.0 33.0 37.0 30.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 5.0 21 9.0 22 7.0 23 7.0 24 9.0 25 16.0 26 17.0 27 28.0 28 28.0 29 40.0 30 42.0 31 76.0 32 97.0 33 126.0 34 192.0 35 395.0 36 841.0 37 1134.0 38 899.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 77.825 6.550000000000001 6.875000000000001 8.75 2 54.25 25.874999999999996 12.85 7.025 3 32.074999999999996 33.300000000000004 18.25 16.375 4 32.975 26.325 19.400000000000002 21.3 5 23.9 31.7 25.8 18.6 6 19.175 37.5 26.8 16.525000000000002 7 34.25 29.599999999999998 20.0 16.150000000000002 8 32.5 27.450000000000003 21.7 18.35 9 24.9 26.025 28.9 20.175 10-11 25.5375 27.8375 26.224999999999998 20.4 12-13 28.849999999999998 23.75 26.924999999999997 20.474999999999998 14-15 23.674999999999997 26.737499999999997 29.612500000000004 19.975 16-17 26.700000000000003 30.025000000000002 24.925 18.35 18-19 23.775 27.150000000000002 25.912499999999998 23.1625 20-21 26.2875 26.5625 26.125 21.025 22-23 27.762500000000003 23.45 27.450000000000003 21.337500000000002 24-25 26.1625 24.8125 27.5875 21.4375 26-27 27.3625 25.7625 27.85 19.025 28-29 25.7625 26.05 26.575 21.6125 30-31 28.525 25.275 25.362499999999997 20.837500000000002 32-33 24.837500000000002 28.475 26.5875 20.1 34-35 27.0625 24.962500000000002 26.950000000000003 21.025 36-37 24.675 24.775 28.1875 22.3625 38-39 25.7 24.375 29.912499999999998 20.0125 40-41 25.874999999999996 26.0375 27.3625 20.724999999999998 42-43 24.837500000000002 28.525 25.662499999999998 20.974999999999998 44-45 23.45 26.200000000000003 28.462500000000002 21.8875 46-47 24.95 23.275000000000002 27.6375 24.1375 48-49 24.7875 25.45 29.312500000000004 20.45 50-51 25.1 26.0 27.675 21.224999999999998 52-53 26.887499999999996 25.7 26.174999999999997 21.2375 54-55 26.0375 26.5 26.5625 20.9 56-57 25.5625 25.0375 28.1375 21.2625 58-59 23.925 24.0 29.849999999999998 22.225 60-61 26.1625 24.05 28.499999999999996 21.2875 62-63 22.5625 28.599999999999998 29.312500000000004 19.525000000000002 64-65 24.6625 25.837500000000002 28.0875 21.4125 66-67 25.900000000000002 27.6875 26.937499999999996 19.475 68-69 24.762500000000003 24.8625 28.349999999999998 22.025 70-71 26.82011508631474 25.156367275456592 26.682511883912934 21.341005754315738 72-73 26.871473354231973 25.16614420062696 27.42319749216301 20.539184952978058 74-75 23.80593262946204 26.59627953745601 28.431372549019606 21.166415284062342 76-77 22.898258015652612 26.495834385256252 28.932087856601868 21.67381974248927 78-79 26.197111730428173 24.119584494552825 28.52799594628832 21.15530782873068 80-81 25.56810968642884 27.320045702678687 28.386441538656847 18.725403072235622 82-83 25.283982131461393 24.62029355456286 29.240587109125716 20.85513720485003 84-85 23.715566944266495 23.215887251761693 30.147341447789877 22.921204356181935 86-87 22.87179487179487 26.615384615384613 29.97435897435897 20.53846153846154 88-89 22.76923076923077 29.384615384615387 28.46153846153846 19.384615384615383 90-91 26.064102564102566 25.73076923076923 29.05128205128205 19.153846153846153 92-93 23.717948717948715 27.487179487179485 28.94871794871795 19.846153846153847 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 12.0 18 12.0 19 1.0 20 1.0 21 1.0 22 2.5 23 5.0 24 4.0 25 2.5 26 4.5 27 6.0 28 9.0 29 11.5 30 15.0 31 19.0 32 27.5 33 47.0 34 61.5 35 80.5 36 101.5 37 112.5 38 130.5 39 146.0 40 161.0 41 180.0 42 183.0 43 178.0 44 190.5 45 197.5 46 211.5 47 219.0 48 188.5 49 185.5 50 206.5 51 197.5 52 167.0 53 167.0 54 158.0 55 107.0 56 65.5 57 51.5 58 58.0 59 56.0 60 50.5 61 47.0 62 34.5 63 32.0 64 33.5 65 27.0 66 20.0 67 18.0 68 13.5 69 10.0 70 8.0 71 4.0 72 6.5 73 6.0 74 3.5 75 2.5 76 0.5 77 0.0 78 0.0 79 0.5 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 6.0 71 5.0 72 3.0 73 4.0 74 8.0 75 7.0 76 12.0 77 5.0 78 6.0 79 4.0 80 3.0 81 14.0 82 11.0 83 7.0 84 5.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3900.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.0 #Duplication Level Percentage of deduplicated Percentage of total 1 94.57831325301204 78.5 2 3.283132530120482 5.45 3 0.8132530120481927 2.025 4 0.2710843373493976 0.8999999999999999 5 0.15060240963855423 0.625 6 0.18072289156626506 0.8999999999999999 7 0.12048192771084339 0.7000000000000001 8 0.06024096385542169 0.4 9 0.030120481927710847 0.22499999999999998 >10 0.4518072289156626 7.5249999999999995 >50 0.06024096385542169 2.75 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 58 1.4500000000000002 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 52 1.3 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 39 0.975 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 34 0.8500000000000001 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 27 0.675 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 26 0.65 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 24 0.6 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 20 0.5 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 18 0.44999999999999996 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 18 0.44999999999999996 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 18 0.44999999999999996 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 17 0.42500000000000004 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 15 0.375 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 14 0.35000000000000003 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 11 0.27499999999999997 No Hit CTGAGCCGTGCTCTCTTCTGTGTACATCTTCATGTCATTTTAGCGATTCT 10 0.25 No Hit GGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTC 10 0.25 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 9 0.22499999999999998 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 8 0.2 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 8 0.2 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 7 0.17500000000000002 No Hit AATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCC 7 0.17500000000000002 No Hit GACCACAGCTATCACACACAGGCAAAACACAGCTGATTCGTGTACTCGAT 7 0.17500000000000002 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 7 0.17500000000000002 No Hit AGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATGAGCCT 6 0.15 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 6 0.15 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 6 0.15 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 6 0.15 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 6 0.15 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 6 0.15 No Hit GGTCTTGATCCCTCTGTGTTTCCCGTGTAACGGCTACTGATCCAGTGGTT 5 0.125 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 5 0.125 No Hit GAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGG 5 0.125 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 5 0.125 No Hit GAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCACCC 15 8.997531E-4 86.299995 7 AGGCACC 15 8.997531E-4 86.299995 6 ACCTAGG 15 8.997531E-4 86.299995 2 CCTAGGC 15 8.997531E-4 86.299995 3 CTAGGCA 20 0.002818753 64.725 4 CACCCAG 20 0.002818753 64.725 9 GCACCCA 20 0.002818753 64.725 8 TACCTAG 20 0.002818753 64.725 1 TAGGCAC 20 0.002818753 64.725 5 GGGGAGT 20 8.142527E-4 43.149998 52-53 >>END_MODULE Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90548 READS because READLEN < 1 Read 90548 spots for ERR6133375.sra Written 90548 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra Rejected 90538 READS because READLEN < 1 Read 90538 spots for ERR6133375.sra Written 90538 spots for ERR6133375.sra SRR ids: ['ERR6133375.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ir5beqgg ERR6133375.sra spots: 1810770 blocks: [[1, 90538], [90539, 181076], [181077, 271614], [271615, 362152], [362153, 452690], [452691, 543228], [543229, 633766], [633767, 724304], [724305, 814842], [814843, 905380], [905381, 995918], [995919, 1086456], [1086457, 1176994], [1176995, 1267532], [1267533, 1358070], [1358071, 1448608], [1448609, 1539146], [1539147, 1629684], [1629685, 1720222], [1720223, 1810770]] ERR6133375 file size 399566 ERR6133375 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133375 ERR6133375_1.fastq Input file: ERR6133375_1.fastq trimmed: ERR6133375-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 01:59:30 2024 >> started Sat Dec 7 01:59:32 2024 >> done (1.414s) 1810770 reads processed; of these: 65 ( 0.00%) short reads filtered out after trimming by size control 1 ( 0.00%) empty reads filtered out after trimming by size control 1810704 (100.00%) reads available; of these: 76006 ( 4.20%) trimmed reads available after processing 1734698 (95.80%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 4 0.00% 20 5 0.00% 21 1 0.00% 22 2 0.00% 23 2 0.00% 24 1 0.00% 25 1 0.00% 26 2 0.00% 27 1 0.00% 28 2 0.00% 29 14 0.00% 30 0 0.00% 31 1 0.00% 32 2 0.00% 33 1 0.00% 34 1 0.00% 35 2 0.00% 36 1 0.00% 37 4 0.00% 38 4 0.00% 39 7 0.00% 40 9 0.00% 41 9 0.00% 42 2 0.00% 43 5 0.00% 44 9 0.00% 45 12 0.00% 46 24 0.00% 47 48 0.00% 48 112 0.01% 49 572 0.03% 50 160 0.01% 51 156 0.01% 52 174 0.01% 53 211 0.01% 54 231 0.01% 55 292 0.02% 56 293 0.02% 57 353 0.02% 58 399 0.02% 59 406 0.02% 60 538 0.03% 61 432 0.02% 62 480 0.03% 63 618 0.03% 64 592 0.03% 65 617 0.03% 66 645 0.04% 67 713 0.04% 68 845 0.05% 69 442 0.02% 70 3293 0.18% 71 3212 0.18% 72 4087 0.23% 73 3591 0.20% 74 3832 0.21% 75 4222 0.23% 76 3708 0.20% 77 3778 0.21% 78 4623 0.26% 79 4947 0.27% 80 4818 0.27% 81 4675 0.26% 82 5537 0.31% 83 7164 0.40% 84 5826 0.32% 85 3147 0.17% 86 3577 0.20% 87 4188 0.23% 88 5363 0.30% 89 5263 0.29% 90 6999 0.39% 91 8412 0.46% 92 8432 0.47% 93 1688549 93.25% 1810704 reads passed initial QC criterion=sequence-density sequence-density=0.48 sequence-density-rank=1 fanout-score=4.38 fanout-score-rank=28 prefix-density=0.64 prefix-fanout=3.3 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.08 sequence-density-rank=15 fanout-score=57.04 fanout-score-rank=1 prefix-density=0.39 prefix-fanout=11.2 sequence=AGAAGAAGGACCCAACCGGCGCCAAGGTCACCAAGGCGGCTGCCAAGAAGAAATGATTTGTTCTGTGTTGGGGATTGAGATGTCTGGCTGCAGTTTTAGTGTTTACTAAGTTTTGTCTATGTGCATTGCTCTTATTGCTGAGACTCTTGAAACTATGTAAGCAATGTTCATGTCTGTCGTTTAAATACTGTATTCCTATTTCGGTTCGGTTAA Started job on | Dec 07 01:59:45 Started mapping on | Dec 07 01:59:45 Finished on | Dec 07 01:59:50 Mapping speed, Million of reads per hour | 1303.71 Number of input reads | 1810704 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 1319471 Uniquely mapped reads % | 72.87% Average mapped length | 91.72 Number of splices: Total | 109815 Number of splices: Annotated (sjdb) | 92588 Number of splices: GT/AG | 103388 Number of splices: GC/AG | 2190 Number of splices: AT/AC | 31 Number of splices: Non-canonical | 4206 Mismatch rate per base, % | 0.50% Deletion rate per base | 0.04% Deletion average length | 1.74 Insertion rate per base | 0.02% Insertion average length | 1.73 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 426225 % of reads mapped to multiple loci | 23.54% Number of reads mapped to too many loci | 10550 % of reads mapped to too many loci | 0.58% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.93% % of reads unmapped: other | 0.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 65008 65008 65008 N_multimapping 426225 426225 426225 N_noFeature 58997 73648 1258274 N_ambiguous 51000 4505 96 UnstrandedReadsAssigned:1209474 PositiveStrandReadsAssigned:1241318 NegativeStrandReadsAssigned:61101 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133375 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133375-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,810,704 reads, 1,517,224 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 953 rounds 52973 ERR6133375.ke.tsv 35125 ERR6133375.se.tsv 88098 total ==> ERR6133375.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 29 18.6262 PNS24243 293 194 0 0 KQK14069 1603 1504 17 9.96052 KQK14071 474 375 0 0 ==> ERR6133375.se.tsv <== BRADI_1g14170v3 17 BRADI_1g53295v3 56 BRADI_1g59795v3 8 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 23 BRADI_1g74790v3 19 BRADI_1g09890v3 0 BRADI_1g77505v3 39 BRADI_1g48960v3 0 ERR6133375 completed mapping pipeline successfully