Starting /dee2/code/volunteer_pipeline.sh ERR6133378 current disk space = 1547905302528 free memory = 1599843468 ERR6133378 SRAfilesize 87e288f77740ad3353c4a88113bf387a ERR6133378.sra ERR6133378.sra file validated ERR6133378 is single end ERR6133378 is conventional basespace ERR6133378 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133378_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.911 37.0 37.0 37.0 37.0 37.0 2 36.8435 37.0 37.0 37.0 37.0 37.0 3 36.6015 37.0 37.0 37.0 37.0 37.0 4 36.07775 37.0 37.0 37.0 33.0 37.0 5 36.0415 37.0 37.0 37.0 33.0 37.0 6 36.21575 37.0 37.0 37.0 33.0 37.0 7 38.115 40.0 37.0 40.0 33.0 40.0 8 38.0845 40.0 37.0 40.0 33.0 40.0 9 38.115 40.0 37.0 40.0 37.0 40.0 10-11 38.080875 40.0 37.0 40.0 37.0 40.0 12-13 38.101 40.0 37.0 40.0 37.0 40.0 14-15 38.07275 40.0 37.0 40.0 37.0 40.0 16-17 38.005125 40.0 37.0 40.0 33.0 40.0 18-19 37.8515 40.0 37.0 40.0 33.0 40.0 20-21 37.793625000000006 40.0 37.0 40.0 33.0 40.0 22-23 37.737125 40.0 37.0 40.0 33.0 40.0 24-25 37.564375 40.0 37.0 40.0 33.0 40.0 26-27 37.407375 37.0 37.0 40.0 33.0 40.0 28-29 37.322 37.0 37.0 40.0 33.0 40.0 30-31 37.160125 37.0 37.0 40.0 33.0 40.0 32-33 36.915499999999994 37.0 37.0 40.0 33.0 40.0 34-35 36.826499999999996 37.0 37.0 40.0 33.0 40.0 36-37 36.82575 37.0 37.0 40.0 33.0 40.0 38-39 36.913124999999994 37.0 37.0 40.0 33.0 40.0 40-41 37.117374999999996 37.0 37.0 40.0 33.0 40.0 42-43 36.987875 37.0 37.0 40.0 33.0 40.0 44-45 36.896375 37.0 37.0 40.0 33.0 40.0 46-47 36.697625 37.0 37.0 40.0 33.0 40.0 48-49 36.5715 37.0 37.0 38.5 33.0 40.0 50-51 36.434 37.0 37.0 37.0 33.0 40.0 52-53 36.22925 37.0 37.0 37.0 33.0 40.0 54-55 36.1105 37.0 37.0 37.0 33.0 40.0 56-57 35.927625 37.0 37.0 37.0 33.0 40.0 58-59 35.798375 37.0 37.0 37.0 33.0 38.5 60-61 35.679249999999996 37.0 37.0 37.0 33.0 37.0 62-63 35.306125 37.0 33.0 37.0 33.0 37.0 64-65 35.14125 37.0 33.0 37.0 33.0 37.0 66-67 35.1815 37.0 33.0 37.0 33.0 37.0 68-69 34.158125 35.0 33.0 37.0 30.0 37.0 70-71 34.467332310966455 37.0 33.0 37.0 33.0 37.0 72-73 34.93013764529428 37.0 33.0 37.0 33.0 37.0 74-75 34.93678234065699 37.0 33.0 37.0 33.0 37.0 76-77 34.87653981143762 37.0 33.0 37.0 33.0 37.0 78-79 34.92772978764387 37.0 33.0 37.0 33.0 37.0 80-81 34.875995552106176 37.0 33.0 37.0 33.0 37.0 82-83 34.45157412047865 37.0 33.0 37.0 33.0 37.0 84-85 34.565613282092855 37.0 33.0 37.0 33.0 37.0 86-87 34.51986249045073 37.0 33.0 37.0 33.0 37.0 88-89 34.419786096256686 37.0 33.0 37.0 33.0 37.0 90-91 34.02699261522791 37.0 33.0 37.0 30.0 37.0 92-93 33.89075630252101 37.0 33.0 37.0 30.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 7.0 21 2.0 22 5.0 23 8.0 24 18.0 25 16.0 26 22.0 27 25.0 28 29.0 29 43.0 30 52.0 31 58.0 32 104.0 33 119.0 34 221.0 35 385.0 36 956.0 37 1087.0 38 818.0 39 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 84.65 4.475 3.55 7.324999999999999 2 65.525 19.75 9.45 5.2749999999999995 3 35.6 37.925 13.750000000000002 12.725 4 34.050000000000004 27.425 18.275 20.25 5 23.599999999999998 32.875 24.4 19.125 6 19.925 38.2 26.625 15.25 7 37.05 29.5 18.5 14.95 8 32.725 30.0 22.075 15.2 9 26.5 27.575 27.224999999999998 18.7 10-11 27.025 27.6 26.187500000000004 19.1875 12-13 30.599999999999998 24.9125 26.900000000000002 17.5875 14-15 23.45 27.375 29.062500000000004 20.1125 16-17 25.662499999999998 32.4 24.775 17.1625 18-19 24.587500000000002 27.125 27.175 21.1125 20-21 25.75 26.487500000000004 26.2875 21.475 22-23 26.950000000000003 24.3 26.3625 22.3875 24-25 26.337500000000002 23.9375 28.425 21.3 26-27 26.375 24.587500000000002 29.462500000000002 19.575 28-29 26.474999999999998 26.0375 26.687499999999996 20.8 30-31 27.875 25.374999999999996 26.450000000000003 20.3 32-33 24.0625 27.35 28.1125 20.474999999999998 34-35 26.1625 24.825 27.85 21.1625 36-37 24.65 24.175 28.575 22.6 38-39 26.0625 24.7 29.612500000000004 19.625 40-41 26.35 24.725 27.437499999999996 21.4875 42-43 24.925 28.3875 27.0 19.6875 44-45 24.0 24.4125 28.675 22.912499999999998 46-47 24.125 24.0625 27.787499999999998 24.025 48-49 25.137500000000003 24.325 30.25 20.2875 50-51 24.95 26.424999999999997 28.625 20.0 52-53 26.337500000000002 26.737499999999997 27.037499999999998 19.8875 54-55 25.0625 27.975 27.3375 19.625 56-57 25.162499999999998 25.0375 29.012500000000003 20.7875 58-59 23.962500000000002 24.6625 28.625 22.75 60-61 25.874999999999996 24.175 29.299999999999997 20.65 62-63 23.375 27.3875 29.549999999999997 19.6875 64-65 24.962500000000002 25.0125 28.8875 21.1375 66-67 25.775 26.625 27.3 20.3 68-69 23.8375 25.525 28.6875 21.95 70-71 26.182136602451838 24.39329497122842 27.970978233675257 21.453590192644484 72-73 27.28526645768025 24.163009404388713 27.699059561128525 20.852664576802507 74-75 23.08756919979869 26.91243080020131 28.459989934574736 21.540010065425264 76-77 22.996339770289033 26.984728007068032 28.764356935504225 21.25457528713871 78-79 25.625473843821077 24.538792014152136 29.163507707859488 20.6722264341673 80-81 24.41742654508612 26.595744680851062 29.305977710233027 19.680851063829788 82-83 24.638233054074636 24.333587204874334 30.33764914953034 20.69053059152069 84-85 24.309532900598192 23.80043273514064 30.16418480336006 21.725849560901107 86-87 23.52941176470588 25.07002801120448 31.117901706137 20.282658517952637 88-89 23.1729055258467 28.50776674306086 29.526355996944233 18.792971734148207 90-91 25.744843391902215 25.18461930226636 29.411764705882355 19.65877259994907 92-93 23.9877769289534 26.177743824802647 29.67914438502674 20.155334861217213 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 6.5 18 6.5 19 1.5 20 1.0 21 0.5 22 0.5 23 2.0 24 5.5 25 6.0 26 6.5 27 8.5 28 13.5 29 17.5 30 14.5 31 15.0 32 30.0 33 52.0 34 70.5 35 94.5 36 105.0 37 108.5 38 128.5 39 161.0 40 191.0 41 180.5 42 175.5 43 190.0 44 183.0 45 185.5 46 214.5 47 201.0 48 180.0 49 199.0 50 194.0 51 183.0 52 173.0 53 167.5 54 149.0 55 101.5 56 69.0 57 60.5 58 64.0 59 64.0 60 52.5 61 49.0 62 47.0 63 38.0 64 29.5 65 25.0 66 21.0 67 15.5 68 14.0 69 8.0 70 3.5 71 3.0 72 2.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 6.0 71 3.0 72 7.0 73 6.0 74 8.0 75 7.0 76 3.0 77 2.0 78 2.0 79 7.0 80 2.0 81 5.0 82 6.0 83 6.0 84 3.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3927.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.6 #Duplication Level Percentage of deduplicated Percentage of total 1 94.23758865248227 79.725 2 3.57565011820331 6.05 3 0.9160756501182032 2.325 4 0.38416075650118203 1.3 5 0.14775413711583923 0.625 6 0.14775413711583923 0.75 7 0.08865248226950355 0.525 8 0.0591016548463357 0.4 9 0.0 0.0 >10 0.44326241134751776 8.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 45 1.125 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 44 1.0999999999999999 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 35 0.8750000000000001 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 27 0.675 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 26 0.65 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 21 0.525 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 19 0.475 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 19 0.475 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 17 0.42500000000000004 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 16 0.4 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 16 0.4 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 13 0.325 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 12 0.3 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 12 0.3 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 10 0.25 No Hit GGATCGGTTGAAGCGTGCGGCGTCTCTGTCTATGTATTACTGTTTTATGC 8 0.2 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 8 0.2 No Hit GGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTC 7 0.17500000000000002 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 7 0.17500000000000002 No Hit GCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGA 7 0.17500000000000002 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 6 0.15 No Hit AATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCC 6 0.15 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 6 0.15 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 6 0.15 No Hit GGGGAACGCGAAATCACTTTAGGTTTTGTTGATTTATTGCGCGACGATTT 6 0.15 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 5 0.125 No Hit GGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTC 5 0.125 No Hit GTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCA 5 0.125 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 5 0.125 No Hit GGGACGCATGCAACGACCATCTACATATAGCTACTCGATCTACCGCTACC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0125 74-75 0.05 0.0 0.0 0.0 0.025 76-77 0.05 0.0 0.0 0.0 0.025 78-79 0.05 0.0 0.0 0.0 0.025 80-81 0.0625 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147213 READS because READLEN < 1 Read 147213 spots for ERR6133378.sra Written 147213 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra Rejected 147198 READS because READLEN < 1 Read 147198 spots for ERR6133378.sra Written 147198 spots for ERR6133378.sra SRR ids: ['ERR6133378.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_mp6kp252 ERR6133378.sra spots: 2943975 blocks: [[1, 147198], [147199, 294396], [294397, 441594], [441595, 588792], [588793, 735990], [735991, 883188], [883189, 1030386], [1030387, 1177584], [1177585, 1324782], [1324783, 1471980], [1471981, 1619178], [1619179, 1766376], [1766377, 1913574], [1913575, 2060772], [2060773, 2207970], [2207971, 2355168], [2355169, 2502366], [2502367, 2649564], [2649565, 2796762], [2796763, 2943975]] ERR6133378 file size 651542 ERR6133378 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133378 ERR6133378_1.fastq Input file: ERR6133378_1.fastq trimmed: ERR6133378-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:09:52 2024 >> started Sat Dec 7 02:09:54 2024 >> done (2.394s) 2943975 reads processed; of these: 171 ( 0.01%) short reads filtered out after trimming by size control 8 ( 0.00%) empty reads filtered out after trimming by size control 2943796 (99.99%) reads available; of these: 121310 ( 4.12%) trimmed reads available after processing 2822486 (95.88%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 15 0.00% 19 18 0.00% 20 9 0.00% 21 9 0.00% 22 13 0.00% 23 8 0.00% 24 2 0.00% 25 3 0.00% 26 10 0.00% 27 15 0.00% 28 5 0.00% 29 21 0.00% 30 5 0.00% 31 8 0.00% 32 7 0.00% 33 8 0.00% 34 6 0.00% 35 1 0.00% 36 3 0.00% 37 7 0.00% 38 16 0.00% 39 17 0.00% 40 29 0.00% 41 21 0.00% 42 12 0.00% 43 8 0.00% 44 17 0.00% 45 28 0.00% 46 56 0.00% 47 71 0.00% 48 159 0.01% 49 976 0.03% 50 275 0.01% 51 262 0.01% 52 282 0.01% 53 363 0.01% 54 410 0.01% 55 472 0.02% 56 500 0.02% 57 659 0.02% 58 539 0.02% 59 665 0.02% 60 868 0.03% 61 670 0.02% 62 730 0.02% 63 955 0.03% 64 944 0.03% 65 925 0.03% 66 1003 0.03% 67 1158 0.04% 68 1340 0.05% 69 653 0.02% 70 4023 0.14% 71 4380 0.15% 72 4951 0.17% 73 4673 0.16% 74 5052 0.17% 75 5460 0.19% 76 4995 0.17% 77 5265 0.18% 78 6218 0.21% 79 6784 0.23% 80 6149 0.21% 81 6464 0.22% 82 7571 0.26% 83 9806 0.33% 84 8522 0.29% 85 4919 0.17% 86 5707 0.19% 87 6544 0.22% 88 8513 0.29% 89 8469 0.29% 90 11295 0.38% 91 13273 0.45% 92 13982 0.47% 93 2765525 93.94% 2943796 reads passed initial QC criterion=sequence-density sequence-density=0.72 sequence-density-rank=1 fanout-score=3.95 fanout-score-rank=26 prefix-density=0.90 prefix-fanout=3.2 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=29 fanout-score=367.64 fanout-score-rank=1 prefix-density=0.68 prefix-fanout=8.3 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTTCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAATAGATACAGGGTCTCAAACCTTGCTATAGAGTTTTTGCTT Started job on | Dec 07 02:10:08 Started mapping on | Dec 07 02:10:08 Finished on | Dec 07 02:10:14 Mapping speed, Million of reads per hour | 1766.28 Number of input reads | 2943796 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 2305025 Uniquely mapped reads % | 78.30% Average mapped length | 91.96 Number of splices: Total | 169410 Number of splices: Annotated (sjdb) | 148743 Number of splices: GT/AG | 163509 Number of splices: GC/AG | 3409 Number of splices: AT/AC | 39 Number of splices: Non-canonical | 2453 Mismatch rate per base, % | 0.48% Deletion rate per base | 0.03% Deletion average length | 1.87 Insertion rate per base | 0.01% Insertion average length | 1.86 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 557201 % of reads mapped to multiple loci | 18.93% Number of reads mapped to too many loci | 13453 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.26% % of reads unmapped: other | 0.05% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 81570 81570 81570 N_multimapping 557201 557201 557201 N_noFeature 97967 118926 2206281 N_ambiguous 84583 6798 198 UnstrandedReadsAssigned:2122475 PositiveStrandReadsAssigned:2179301 NegativeStrandReadsAssigned:98546 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133378 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133378-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,943,796 reads, 2,554,322 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,027 rounds 52973 ERR6133378.ke.tsv 35125 ERR6133378.se.tsv 88098 total ==> ERR6133378.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 83 32.2464 PNS24243 293 194 1 2.74761 KQK14069 1603 1504 30 10.6324 KQK14071 474 375 0 0 ==> ERR6133378.se.tsv <== BRADI_1g14170v3 30 BRADI_1g53295v3 68 BRADI_1g59795v3 33 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 32 BRADI_1g74790v3 18 BRADI_1g09890v3 0 BRADI_1g77505v3 64 BRADI_1g48960v3 0 ERR6133378 completed mapping pipeline successfully