Starting /dee2/code/volunteer_pipeline.sh ERR6133379 current disk space = 1547797409792 free memory = 1407027932 ERR6133379 SRAfilesize d74e37638dd0ded50003d22e1dabef26 ERR6133379.sra ERR6133379.sra file validated ERR6133379 is single end ERR6133379 is conventional basespace ERR6133379 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133379_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.9015 37.0 37.0 37.0 37.0 37.0 2 36.819 37.0 37.0 37.0 37.0 37.0 3 36.548 37.0 37.0 37.0 37.0 37.0 4 35.939 37.0 37.0 37.0 33.0 37.0 5 36.06125 37.0 37.0 37.0 33.0 37.0 6 36.23675 37.0 37.0 37.0 33.0 37.0 7 37.9915 40.0 37.0 40.0 33.0 40.0 8 38.00525 40.0 37.0 40.0 33.0 40.0 9 38.03825 40.0 37.0 40.0 37.0 40.0 10-11 37.95725 40.0 37.0 40.0 35.0 40.0 12-13 37.999625 40.0 37.0 40.0 37.0 40.0 14-15 37.969375 40.0 37.0 40.0 35.0 40.0 16-17 37.905 40.0 37.0 40.0 35.0 40.0 18-19 37.745000000000005 40.0 37.0 40.0 33.0 40.0 20-21 37.630375 38.5 37.0 40.0 33.0 40.0 22-23 37.584375 37.0 37.0 40.0 33.0 40.0 24-25 37.431124999999994 37.0 37.0 40.0 33.0 40.0 26-27 37.3005 37.0 37.0 40.0 33.0 40.0 28-29 37.198499999999996 37.0 37.0 40.0 33.0 40.0 30-31 37.1255 37.0 37.0 40.0 33.0 40.0 32-33 36.823750000000004 37.0 37.0 40.0 33.0 40.0 34-35 36.648624999999996 37.0 37.0 40.0 33.0 40.0 36-37 36.6165 37.0 37.0 40.0 33.0 40.0 38-39 36.79075 37.0 37.0 40.0 33.0 40.0 40-41 36.935375 37.0 37.0 40.0 33.0 40.0 42-43 36.87475 37.0 37.0 40.0 33.0 40.0 44-45 36.862125 37.0 37.0 40.0 33.0 40.0 46-47 36.626625000000004 37.0 37.0 40.0 33.0 40.0 48-49 36.541 37.0 37.0 40.0 33.0 40.0 50-51 36.462374999999994 37.0 37.0 37.0 33.0 40.0 52-53 36.248875 37.0 37.0 37.0 33.0 40.0 54-55 36.093 37.0 37.0 37.0 33.0 40.0 56-57 35.8995 37.0 37.0 37.0 33.0 40.0 58-59 35.782624999999996 37.0 37.0 37.0 33.0 38.5 60-61 35.684875000000005 37.0 35.0 37.0 33.0 37.0 62-63 35.420375 37.0 33.0 37.0 33.0 37.0 64-65 35.190375 37.0 33.0 37.0 33.0 37.0 66-67 35.112125 37.0 33.0 37.0 33.0 37.0 68-69 34.16975 35.0 33.0 37.0 30.0 37.0 70-71 34.432420767593285 37.0 33.0 37.0 33.0 37.0 72-73 34.824275089830365 37.0 33.0 37.0 33.0 37.0 74-75 34.821518768065765 37.0 33.0 37.0 33.0 37.0 76-77 34.75101973545277 37.0 33.0 37.0 33.0 37.0 78-79 34.78269056283382 37.0 33.0 37.0 33.0 37.0 80-81 34.73240586215866 37.0 33.0 37.0 33.0 37.0 82-83 34.413036327190355 37.0 33.0 37.0 33.0 37.0 84-85 34.450326307434516 37.0 33.0 37.0 33.0 37.0 86-87 34.47071611253197 37.0 33.0 37.0 33.0 37.0 88-89 34.41828644501279 37.0 33.0 37.0 33.0 37.0 90-91 34.0888746803069 37.0 33.0 37.0 30.0 37.0 92-93 33.95907928388746 37.0 33.0 37.0 30.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 8.0 21 7.0 22 4.0 23 9.0 24 18.0 25 19.0 26 18.0 27 30.0 28 29.0 29 43.0 30 57.0 31 86.0 32 100.0 33 122.0 34 219.0 35 394.0 36 933.0 37 1022.0 38 865.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 85.8 4.05 3.5999999999999996 6.550000000000001 2 66.7 19.175 9.700000000000001 4.425 3 36.25 36.1 15.15 12.5 4 35.325 26.450000000000003 18.95 19.275000000000002 5 23.674999999999997 33.025 24.25 19.05 6 21.95 36.775000000000006 25.650000000000002 15.625 7 37.35 29.625 19.075 13.950000000000001 8 33.45 30.85 22.425 13.275 9 26.8 28.175 26.474999999999998 18.55 10-11 26.237500000000004 28.499999999999996 27.474999999999998 17.7875 12-13 29.7 24.775 28.462500000000002 17.0625 14-15 22.8375 28.1375 29.799999999999997 19.225 16-17 25.025 32.05 26.05 16.875 18-19 24.1625 26.7625 28.1125 20.962500000000002 20-21 25.1875 25.8125 27.787499999999998 21.212500000000002 22-23 26.474999999999998 23.95 28.537499999999998 21.0375 24-25 25.874999999999996 24.4125 29.2875 20.424999999999997 26-27 26.1 24.9375 29.2 19.7625 28-29 25.45 26.05 28.1 20.4 30-31 26.6125 25.074999999999996 27.900000000000002 20.4125 32-33 24.4375 27.487499999999997 28.225 19.85 34-35 25.2 24.5625 29.6375 20.599999999999998 36-37 25.0125 24.825 29.225 20.9375 38-39 25.55 24.587500000000002 30.099999999999998 19.7625 40-41 26.137500000000003 24.9 28.6125 20.349999999999998 42-43 25.275 26.737499999999997 28.225 19.7625 44-45 23.8375 25.2125 29.849999999999998 21.099999999999998 46-47 24.1625 23.974999999999998 29.125 22.7375 48-49 24.762500000000003 25.587500000000002 29.9 19.75 50-51 24.925 25.412499999999998 28.9875 20.674999999999997 52-53 25.2 25.3 29.062500000000004 20.4375 54-55 23.65 26.5625 29.362500000000004 20.424999999999997 56-57 24.725 25.825 30.175 19.275000000000002 58-59 23.9125 25.2375 29.0875 21.762500000000003 60-61 25.112499999999997 23.1625 30.837500000000002 20.8875 62-63 22.55 25.474999999999998 32.1625 19.8125 64-65 25.337500000000002 24.95 29.475 20.2375 66-67 25.374999999999996 25.4375 29.4875 19.7 68-69 23.7 25.4875 29.775000000000002 21.0375 70-71 25.497310146378084 24.54647816839735 28.53747028650069 21.418741398723885 72-73 25.855370347161298 24.714876550946233 29.38964782554205 20.04010527635042 74-75 23.957286432160803 25.690954773869347 30.452261306532662 19.899497487437188 76-77 22.87046370967742 24.92439516129032 31.791834677419356 20.413306451612904 78-79 24.737706990266716 24.636581974465933 30.47655163696119 20.149159398306157 80-81 24.130931235726973 26.820603907637658 30.499873128647554 18.54859172798782 82-83 24.064647493000763 25.23542886230593 30.38941206413846 20.31051158055485 84-85 23.987220447284344 24.421725239616613 31.194888178913736 20.396166134185304 86-87 23.77237851662404 26.035805626598464 31.278772378516624 18.913043478260867 88-89 22.468030690537084 27.48081841432225 31.41943734015345 18.63171355498721 90-91 24.948849104859335 25.179028132992325 30.71611253196931 19.156010230179028 92-93 22.48081841432225 27.35294117647059 30.498721227621484 19.66751918158568 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 3.5 18 3.5 19 0.5 20 2.0 21 2.5 22 1.0 23 2.0 24 2.5 25 4.0 26 8.0 27 12.0 28 16.0 29 19.0 30 21.5 31 32.0 32 42.5 33 52.0 34 75.5 35 99.0 36 110.5 37 133.0 38 158.5 39 174.5 40 195.5 41 208.0 42 210.0 43 212.0 44 194.0 45 189.0 46 214.5 47 215.5 48 210.0 49 190.0 50 167.5 51 159.0 52 137.5 53 134.5 54 128.5 55 96.0 56 65.0 57 53.0 58 55.0 59 50.5 60 41.0 61 35.5 62 34.5 63 27.5 64 19.0 65 20.5 66 18.5 67 14.0 68 10.0 69 6.5 70 6.0 71 6.0 72 4.5 73 1.5 74 1.0 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 7.0 71 3.0 72 1.0 73 6.0 74 6.0 75 6.0 76 6.0 77 6.0 78 7.0 79 10.0 80 2.0 81 8.0 82 6.0 83 11.0 84 5.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3910.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.175 #Duplication Level Percentage of deduplicated Percentage of total 1 94.47122200170116 83.3 2 3.515735752764389 6.2 3 0.8789339381910972 2.325 4 0.396937907570173 1.4000000000000001 5 0.1984689537850865 0.8750000000000001 6 0.08505812305075135 0.44999999999999996 7 0.05670541536716756 0.35000000000000003 8 0.11341083073433512 0.8 9 0.02835270768358378 0.22499999999999998 >10 0.25517436915225405 4.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 27 0.675 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 25 0.625 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 24 0.6 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 18 0.44999999999999996 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 17 0.42500000000000004 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 17 0.42500000000000004 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 12 0.3 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 12 0.3 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 11 0.27499999999999997 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 9 0.22499999999999998 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 8 0.2 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 8 0.2 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 8 0.2 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 8 0.2 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 7 0.17500000000000002 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 7 0.17500000000000002 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 6 0.15 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 6 0.15 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 6 0.15 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 5 0.125 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 5 0.125 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 5 0.125 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 5 0.125 No Hit GGAGTAAACTAGCATAAATAATTTGTTTGATGCCAGTCATCAAGGTTGAG 5 0.125 No Hit GGGTGTGAGCTGGAGAGACGATCGGGTCTCTCAGCCGGCGTCTTCATCAG 5 0.125 No Hit GGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGTGG 20 0.0027691256 65.015625 1 >>END_MODULE Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150839 READS because READLEN < 1 Read 150839 spots for ERR6133379.sra Written 150839 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra Rejected 150830 READS because READLEN < 1 Read 150830 spots for ERR6133379.sra Written 150830 spots for ERR6133379.sra SRR ids: ['ERR6133379.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_sj6n0_bs ERR6133379.sra spots: 3016609 blocks: [[1, 150830], [150831, 301660], [301661, 452490], [452491, 603320], [603321, 754150], [754151, 904980], [904981, 1055810], [1055811, 1206640], [1206641, 1357470], [1357471, 1508300], [1508301, 1659130], [1659131, 1809960], [1809961, 1960790], [1960791, 2111620], [2111621, 2262450], [2262451, 2413280], [2413281, 2564110], [2564111, 2714940], [2714941, 2865770], [2865771, 3016609]] ERR6133379 file size 666884 ERR6133379 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133379 ERR6133379_1.fastq Input file: ERR6133379_1.fastq trimmed: ERR6133379-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:15:16 2024 >> started Sat Dec 7 02:15:18 2024 >> done (1.950s) 3016609 reads processed; of these: 147 ( 0.00%) short reads filtered out after trimming by size control 8 ( 0.00%) empty reads filtered out after trimming by size control 3016454 (99.99%) reads available; of these: 119716 ( 3.97%) trimmed reads available after processing 2896738 (96.03%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 26 0.00% 20 11 0.00% 21 7 0.00% 22 11 0.00% 23 4 0.00% 24 2 0.00% 25 4 0.00% 26 9 0.00% 27 4 0.00% 28 6 0.00% 29 12 0.00% 30 7 0.00% 31 4 0.00% 32 4 0.00% 33 7 0.00% 34 2 0.00% 35 5 0.00% 36 3 0.00% 37 7 0.00% 38 12 0.00% 39 17 0.00% 40 37 0.00% 41 14 0.00% 42 19 0.00% 43 11 0.00% 44 18 0.00% 45 20 0.00% 46 54 0.00% 47 68 0.00% 48 146 0.00% 49 867 0.03% 50 246 0.01% 51 267 0.01% 52 294 0.01% 53 356 0.01% 54 382 0.01% 55 423 0.01% 56 535 0.02% 57 658 0.02% 58 492 0.02% 59 673 0.02% 60 857 0.03% 61 704 0.02% 62 750 0.02% 63 1026 0.03% 64 916 0.03% 65 913 0.03% 66 1096 0.04% 67 1159 0.04% 68 1506 0.05% 69 616 0.02% 70 5617 0.19% 71 6081 0.20% 72 6640 0.22% 73 6274 0.21% 74 6501 0.22% 75 7198 0.24% 76 6634 0.22% 77 7087 0.23% 78 7904 0.26% 79 8552 0.28% 80 8133 0.27% 81 8566 0.28% 82 9491 0.31% 83 12067 0.40% 84 10437 0.35% 85 4821 0.16% 86 5728 0.19% 87 6429 0.21% 88 8349 0.28% 89 8381 0.28% 90 11048 0.37% 91 12214 0.40% 92 13791 0.46% 93 2813216 93.26% 3016454 reads passed initial QC criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=5.24 fanout-score-rank=25 prefix-density=0.72 prefix-fanout=3.8 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=485.46 fanout-score-rank=1 prefix-density=0.69 prefix-fanout=9.5 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTTCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAA Started job on | Dec 07 02:15:36 Started mapping on | Dec 07 02:15:36 Finished on | Dec 07 02:15:42 Mapping speed, Million of reads per hour | 1809.87 Number of input reads | 3016454 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 2487942 Uniquely mapped reads % | 82.48% Average mapped length | 91.80 Number of splices: Total | 124611 Number of splices: Annotated (sjdb) | 105192 Number of splices: GT/AG | 119587 Number of splices: GC/AG | 2762 Number of splices: AT/AC | 32 Number of splices: Non-canonical | 2230 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.03% Deletion average length | 1.72 Insertion rate per base | 0.01% Insertion average length | 1.74 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 446607 % of reads mapped to multiple loci | 14.81% Number of reads mapped to too many loci | 15292 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.15% % of reads unmapped: other | 0.06% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 81905 81905 81905 N_multimapping 446607 446607 446607 N_noFeature 113094 137994 2378232 N_ambiguous 92961 8190 193 UnstrandedReadsAssigned:2281887 PositiveStrandReadsAssigned:2341758 NegativeStrandReadsAssigned:109517 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133379 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133379-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,016,454 reads, 2,621,596 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,044 rounds 52973 ERR6133379.ke.tsv 35125 ERR6133379.se.tsv 88098 total ==> ERR6133379.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 75 28.2196 PNS24243 293 194 0 0 KQK14069 1603 1504 42.0787 14.4431 KQK14071 474 375 0 0 ==> ERR6133379.se.tsv <== BRADI_1g14170v3 49 BRADI_1g53295v3 38 BRADI_1g59795v3 15 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 33 BRADI_1g74790v3 34 BRADI_1g09890v3 0 BRADI_1g77505v3 98 BRADI_1g48960v3 0 ERR6133379 completed mapping pipeline successfully