Starting /dee2/code/volunteer_pipeline.sh ERR6133381 current disk space = 1547631722496 free memory = 1600346200 ERR6133381 SRAfilesize a426b28b075835e73a38557317596b3b ERR6133381.sra ERR6133381.sra file validated ERR6133381 is single end ERR6133381 is conventional basespace ERR6133381 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133381_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.87 37.0 37.0 37.0 37.0 37.0 2 36.82975 37.0 37.0 37.0 37.0 37.0 3 36.5985 37.0 37.0 37.0 37.0 37.0 4 36.14725 37.0 37.0 37.0 33.0 37.0 5 36.12875 37.0 37.0 37.0 33.0 37.0 6 36.2955 37.0 37.0 37.0 33.0 37.0 7 38.2925 40.0 37.0 40.0 37.0 40.0 8 38.31175 40.0 37.0 40.0 37.0 40.0 9 38.293 40.0 37.0 40.0 37.0 40.0 10-11 38.252750000000006 40.0 37.0 40.0 37.0 40.0 12-13 38.266875 40.0 37.0 40.0 37.0 40.0 14-15 38.189625 40.0 37.0 40.0 37.0 40.0 16-17 38.113749999999996 40.0 37.0 40.0 35.0 40.0 18-19 38.001125 40.0 37.0 40.0 33.0 40.0 20-21 37.94475 40.0 37.0 40.0 33.0 40.0 22-23 37.87675 40.0 37.0 40.0 33.0 40.0 24-25 37.7265 40.0 37.0 40.0 33.0 40.0 26-27 37.60675 40.0 37.0 40.0 33.0 40.0 28-29 37.457375 40.0 37.0 40.0 33.0 40.0 30-31 37.373125 37.0 37.0 40.0 33.0 40.0 32-33 37.127250000000004 37.0 37.0 40.0 33.0 40.0 34-35 36.9095 37.0 37.0 40.0 33.0 40.0 36-37 36.920500000000004 37.0 37.0 40.0 33.0 40.0 38-39 36.984125 37.0 37.0 40.0 33.0 40.0 40-41 37.253875 37.0 37.0 40.0 33.0 40.0 42-43 37.123125 37.0 37.0 40.0 33.0 40.0 44-45 37.070375 37.0 37.0 40.0 33.0 40.0 46-47 36.804375 37.0 37.0 40.0 33.0 40.0 48-49 36.722875 37.0 37.0 40.0 33.0 40.0 50-51 36.632875 37.0 37.0 40.0 33.0 40.0 52-53 36.4675 37.0 37.0 38.5 33.0 40.0 54-55 36.248999999999995 37.0 37.0 37.0 33.0 40.0 56-57 36.025375 37.0 37.0 37.0 33.0 40.0 58-59 35.92725 37.0 37.0 37.0 33.0 40.0 60-61 35.761875 37.0 37.0 37.0 33.0 37.0 62-63 35.405249999999995 37.0 33.0 37.0 33.0 37.0 64-65 35.258875 37.0 33.0 37.0 33.0 37.0 66-67 35.206375 37.0 33.0 37.0 33.0 37.0 68-69 34.280375 35.0 33.0 37.0 30.0 37.0 70-71 34.51699996867953 37.0 33.0 37.0 33.0 37.0 72-73 34.860045827372275 37.0 33.0 37.0 33.0 37.0 74-75 34.77319050484184 37.0 33.0 37.0 33.0 37.0 76-77 34.64603204746267 37.0 33.0 37.0 33.0 37.0 78-79 34.894926757530314 37.0 33.0 37.0 33.0 37.0 80-81 34.67998177557928 37.0 33.0 37.0 33.0 37.0 82-83 34.40019091026956 37.0 33.0 37.0 33.0 37.0 84-85 34.421353311209 37.0 33.0 37.0 33.0 37.0 86-87 34.31646746347941 37.0 33.0 37.0 33.0 37.0 88-89 34.29641434262948 37.0 33.0 37.0 33.0 37.0 90-91 33.97768924302789 37.0 33.0 37.0 27.0 37.0 92-93 33.75803452855246 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 5.0 21 5.0 22 1.0 23 8.0 24 21.0 25 11.0 26 26.0 27 28.0 28 32.0 29 42.0 30 45.0 31 85.0 32 75.0 33 117.0 34 214.0 35 378.0 36 820.0 37 1085.0 38 976.0 39 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 77.97500000000001 5.6000000000000005 5.3 11.125 2 56.25 24.65 12.6 6.5 3 31.374999999999996 36.3 17.925 14.399999999999999 4 33.900000000000006 24.575 20.25 21.275 5 22.45 32.675 25.8 19.075 6 19.400000000000002 37.05 26.35 17.2 7 31.674999999999997 29.549999999999997 21.5 17.275 8 28.825 29.4 25.15 16.625 9 26.25 25.85 27.825 20.075000000000003 10-11 24.975 28.225 27.037499999999998 19.7625 12-13 27.1 25.224999999999998 28.875 18.8 14-15 22.725 25.674999999999997 31.5625 20.0375 16-17 24.9125 31.5125 26.1125 17.4625 18-19 23.8125 26.5375 26.474999999999998 23.175 20-21 24.825 26.400000000000002 27.212500000000002 21.5625 22-23 26.35 24.5625 26.825 22.2625 24-25 26.687499999999996 24.7375 26.5375 22.037499999999998 26-27 25.9625 25.874999999999996 28.462500000000002 19.7 28-29 25.412499999999998 26.2125 27.6125 20.7625 30-31 27.5125 25.3 26.3 20.8875 32-33 23.625 28.425 27.962500000000002 19.9875 34-35 25.3125 24.962500000000002 27.8875 21.837500000000002 36-37 26.0375 24.462500000000002 28.025 21.475 38-39 25.25 25.900000000000002 29.875 18.975 40-41 25.2625 25.2 28.212500000000002 21.325 42-43 24.6625 28.262500000000003 27.224999999999998 19.85 44-45 23.925 26.3125 28.9125 20.849999999999998 46-47 25.0 24.25 27.275 23.474999999999998 48-49 24.65 25.3 29.2375 20.8125 50-51 24.325 26.525 28.8625 20.2875 52-53 25.3125 26.1625 28.487499999999997 20.0375 54-55 24.65 27.6875 27.375 20.2875 56-57 24.762500000000003 25.4875 28.675 21.075 58-59 23.575 25.5 29.4125 21.512500000000003 60-61 24.8625 24.5125 28.925 21.7 62-63 21.587500000000002 28.425 30.725 19.2625 64-65 23.75 27.3125 28.875 20.0625 66-67 24.7375 27.575 28.075 19.6125 68-69 23.8125 26.575 28.925 20.6875 70-71 26.254536353397572 24.65273432611688 28.26930296583657 20.82342635464898 72-73 25.941317214456618 25.38723082735172 28.22062712504722 20.45082483314444 74-75 22.76887871853547 27.32011187388762 29.087210780574623 20.823798627002287 76-77 22.34872713317129 26.59588077267494 29.93475757963413 21.120634514519637 78-79 24.83550509611663 25.106437878983357 29.312346793962067 20.745710230937945 80-81 24.600182030945263 27.05759979196463 28.994929137953452 19.347289039136655 82-83 23.53712936237208 25.583836263447914 30.556284439779585 20.32274993440042 84-85 24.3475957080408 24.188634256192874 29.83176579679428 21.63200423897205 86-87 23.187250996015937 25.710491367861888 30.650730411686588 20.45152722443559 88-89 22.297476759628154 29.60159362549801 30.199203187250994 17.90172642762284 90-91 25.776892430278885 26.46746347941567 28.100929614873838 19.654714475431607 92-93 22.204515272244358 29.58831341301461 28.419654714475435 19.787516600265604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 4.5 18 6.0 19 2.0 20 0.5 21 1.0 22 2.0 23 2.5 24 5.5 25 7.0 26 6.0 27 11.5 28 22.0 29 25.0 30 26.5 31 40.0 32 54.5 33 66.0 34 74.5 35 96.0 36 126.0 37 142.0 38 166.5 39 171.0 40 180.0 41 199.5 42 201.5 43 204.5 44 190.0 45 175.0 46 197.5 47 224.0 48 199.5 49 170.5 50 170.5 51 163.0 52 139.0 53 155.0 54 148.5 55 88.0 56 59.0 57 55.0 58 53.5 59 45.5 60 39.5 61 42.0 62 37.0 63 31.5 64 25.0 65 21.0 66 23.5 67 20.0 68 15.0 69 7.5 70 4.5 71 5.5 72 3.0 73 1.0 74 0.5 75 1.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 9.0 71 13.0 72 15.0 73 20.0 74 20.0 75 9.0 76 11.0 77 19.0 78 17.0 79 17.0 80 9.0 81 18.0 82 24.0 83 15.0 84 19.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3765.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.32499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 94.69674772927044 80.80000000000001 2 3.2522707295634343 5.55 3 0.8203926164664519 2.1 4 0.20509815411661297 0.7000000000000001 5 0.17579841781423966 0.75 6 0.11719894520949312 0.6 7 0.08789920890711983 0.525 8 0.05859947260474656 0.4 9 0.05859947260474656 0.44999999999999996 >10 0.4980955171403457 6.5 >50 0.02929973630237328 1.625 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 65 1.625 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 26 0.65 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 24 0.6 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 21 0.525 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 20 0.5 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 17 0.42500000000000004 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 16 0.4 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 15 0.375 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 15 0.375 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 15 0.375 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 14 0.35000000000000003 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 13 0.325 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 13 0.325 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 11 0.27499999999999997 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 10 0.25 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 10 0.25 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 10 0.25 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 10 0.25 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 9 0.22499999999999998 No Hit GGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTC 9 0.22499999999999998 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 8 0.2 No Hit GGGACGCATGCAACGACCATCTACATATAGCTACTCGATCTACCGCTACC 8 0.2 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 7 0.17500000000000002 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 7 0.17500000000000002 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 7 0.17500000000000002 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 6 0.15 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 6 0.15 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 6 0.15 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 6 0.15 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 5 0.125 No Hit GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC 5 0.125 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 5 0.125 No Hit GTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCA 5 0.125 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 5 0.125 No Hit GACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACAAT 30 0.0060631684 28.662498 14-15 AAAAAAA 45 0.0010815427 25.142542 86-87 >>END_MODULE Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125487 READS because READLEN < 1 Read 125487 spots for ERR6133381.sra Written 125487 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra Rejected 125482 READS because READLEN < 1 Read 125482 spots for ERR6133381.sra Written 125482 spots for ERR6133381.sra SRR ids: ['ERR6133381.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0s30jr9n ERR6133381.sra spots: 2509645 blocks: [[1, 125482], [125483, 250964], [250965, 376446], [376447, 501928], [501929, 627410], [627411, 752892], [752893, 878374], [878375, 1003856], [1003857, 1129338], [1129339, 1254820], [1254821, 1380302], [1380303, 1505784], [1505785, 1631266], [1631267, 1756748], [1756749, 1882230], [1882231, 2007712], [2007713, 2133194], [2133195, 2258676], [2258677, 2384158], [2384159, 2509645]] ERR6133381 file size 551597 ERR6133381 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133381 ERR6133381_1.fastq Input file: ERR6133381_1.fastq trimmed: ERR6133381-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:20:47 2024 >> started Sat Dec 7 02:20:48 2024 >> done (1.856s) 2509645 reads processed; of these: 362 ( 0.01%) short reads filtered out after trimming by size control 5 ( 0.00%) empty reads filtered out after trimming by size control 2509278 (99.99%) reads available; of these: 104110 ( 4.15%) trimmed reads available after processing 2405168 (95.85%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 33 0.00% 19 33 0.00% 20 21 0.00% 21 23 0.00% 22 18 0.00% 23 8 0.00% 24 15 0.00% 25 15 0.00% 26 16 0.00% 27 19 0.00% 28 22 0.00% 29 34 0.00% 30 13 0.00% 31 10 0.00% 32 21 0.00% 33 6 0.00% 34 9 0.00% 35 16 0.00% 36 11 0.00% 37 23 0.00% 38 33 0.00% 39 71 0.00% 40 68 0.00% 41 28 0.00% 42 18 0.00% 43 18 0.00% 44 24 0.00% 45 32 0.00% 46 53 0.00% 47 74 0.00% 48 105 0.00% 49 857 0.03% 50 245 0.01% 51 264 0.01% 52 234 0.01% 53 336 0.01% 54 337 0.01% 55 419 0.02% 56 478 0.02% 57 587 0.02% 58 529 0.02% 59 593 0.02% 60 781 0.03% 61 629 0.03% 62 643 0.03% 63 891 0.04% 64 756 0.03% 65 813 0.03% 66 899 0.04% 67 977 0.04% 68 1265 0.05% 69 706 0.03% 70 10713 0.43% 71 10899 0.43% 72 12844 0.51% 73 11758 0.47% 74 11752 0.47% 75 12532 0.50% 76 10607 0.42% 77 11152 0.44% 78 13117 0.52% 79 13332 0.53% 80 12280 0.49% 81 12172 0.49% 82 14227 0.57% 83 17306 0.69% 84 13548 0.54% 85 4083 0.16% 86 4845 0.19% 87 5483 0.22% 88 6887 0.27% 89 6936 0.28% 90 9445 0.38% 91 10812 0.43% 92 11296 0.45% 93 2247123 89.55% 2509278 reads passed initial QC criterion=sequence-density sequence-density=0.70 sequence-density-rank=1 fanout-score=4.92 fanout-score-rank=27 prefix-density=0.94 prefix-fanout=3.6 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=24 fanout-score=59.89 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=7.7 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTATAGGGAAAACATAATTTATGAGACAATCATACTGTGCAACTCTTGGCTCCATCGATTAATTAATACTCCTTGTTATTGCTTGCATGGTGG Started job on | Dec 07 02:21:01 Started mapping on | Dec 07 02:21:01 Finished on | Dec 07 02:21:07 Mapping speed, Million of reads per hour | 1505.57 Number of input reads | 2509278 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 1843640 Uniquely mapped reads % | 73.47% Average mapped length | 91.06 Number of splices: Total | 114588 Number of splices: Annotated (sjdb) | 97783 Number of splices: GT/AG | 109621 Number of splices: GC/AG | 2280 Number of splices: AT/AC | 22 Number of splices: Non-canonical | 2665 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.03% Deletion average length | 1.77 Insertion rate per base | 0.01% Insertion average length | 1.83 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 545445 % of reads mapped to multiple loci | 21.74% Number of reads mapped to too many loci | 22542 % of reads mapped to too many loci | 0.90% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.77% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 120193 120193 120193 N_multimapping 545445 545445 545445 N_noFeature 84863 102528 1760417 N_ambiguous 71835 6278 216 UnstrandedReadsAssigned:1686942 PositiveStrandReadsAssigned:1734834 NegativeStrandReadsAssigned:83007 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133381 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133381-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,509,278 reads, 2,094,102 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,047 rounds 52973 ERR6133381.ke.tsv 35125 ERR6133381.se.tsv 88098 total ==> ERR6133381.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 60 28.8956 PNS24243 293 194 0 0 KQK14069 1603 1504 63 27.6775 KQK14071 474 375 0 0 ==> ERR6133381.se.tsv <== BRADI_1g14170v3 63 BRADI_1g53295v3 31 BRADI_1g59795v3 16 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 22 BRADI_1g74790v3 7 BRADI_1g09890v3 0 BRADI_1g77505v3 46 BRADI_1g48960v3 0 ERR6133381 completed mapping pipeline successfully