Starting /dee2/code/volunteer_pipeline.sh ERR6133382 current disk space = 1547654000640 free memory = 1363791940 ERR6133382 SRAfilesize 7d7b560a34e5834021e4265cd3bf7bcd ERR6133382.sra ERR6133382.sra file validated ERR6133382 is single end ERR6133382 is conventional basespace ERR6133382 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133382_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.91975 37.0 37.0 37.0 37.0 37.0 2 36.81875 37.0 37.0 37.0 37.0 37.0 3 36.64625 37.0 37.0 37.0 37.0 37.0 4 36.114 37.0 37.0 37.0 33.0 37.0 5 36.096 37.0 37.0 37.0 33.0 37.0 6 36.30125 37.0 37.0 37.0 33.0 37.0 7 38.234 40.0 37.0 40.0 37.0 40.0 8 38.20775 40.0 37.0 40.0 37.0 40.0 9 38.28875 40.0 37.0 40.0 37.0 40.0 10-11 38.189 40.0 37.0 40.0 37.0 40.0 12-13 38.2295 40.0 37.0 40.0 37.0 40.0 14-15 38.191500000000005 40.0 37.0 40.0 37.0 40.0 16-17 38.084875 40.0 37.0 40.0 37.0 40.0 18-19 37.992875 40.0 37.0 40.0 33.0 40.0 20-21 37.872249999999994 40.0 37.0 40.0 33.0 40.0 22-23 37.78175 40.0 37.0 40.0 33.0 40.0 24-25 37.733125 40.0 37.0 40.0 33.0 40.0 26-27 37.591499999999996 40.0 37.0 40.0 33.0 40.0 28-29 37.48475 38.5 37.0 40.0 33.0 40.0 30-31 37.35425 37.0 37.0 40.0 33.0 40.0 32-33 37.158125 37.0 37.0 40.0 33.0 40.0 34-35 36.951 37.0 37.0 40.0 33.0 40.0 36-37 36.933499999999995 37.0 37.0 40.0 33.0 40.0 38-39 37.116 37.0 37.0 40.0 33.0 40.0 40-41 37.3635 37.0 37.0 40.0 33.0 40.0 42-43 37.30875 37.0 37.0 40.0 33.0 40.0 44-45 37.268625 37.0 37.0 40.0 33.0 40.0 46-47 36.998625000000004 37.0 37.0 40.0 33.0 40.0 48-49 36.865375 37.0 37.0 40.0 33.0 40.0 50-51 36.720625 37.0 37.0 40.0 33.0 40.0 52-53 36.514125 37.0 37.0 38.5 33.0 40.0 54-55 36.309875000000005 37.0 37.0 37.0 33.0 40.0 56-57 36.189625 37.0 37.0 37.0 33.0 40.0 58-59 36.03975 37.0 37.0 37.0 33.0 40.0 60-61 35.933625 37.0 37.0 37.0 33.0 38.5 62-63 35.675375 37.0 35.0 37.0 33.0 37.0 64-65 35.338499999999996 37.0 33.0 37.0 33.0 37.0 66-67 35.3375 37.0 33.0 37.0 33.0 37.0 68-69 34.422375 35.0 33.0 37.0 30.0 37.0 70-71 34.709250565042694 37.0 33.0 37.0 33.0 37.0 72-73 35.113006770451705 37.0 33.0 37.0 33.0 37.0 74-75 35.09154786004031 37.0 33.0 37.0 33.0 37.0 76-77 34.98024796937928 37.0 33.0 37.0 33.0 37.0 78-79 35.143146045130045 37.0 33.0 37.0 33.0 37.0 80-81 34.94757256521295 37.0 33.0 37.0 33.0 37.0 82-83 34.62020770449914 37.0 33.0 37.0 33.0 37.0 84-85 34.641843531629576 37.0 33.0 37.0 33.0 37.0 86-87 34.62253178252637 37.0 33.0 37.0 33.0 37.0 88-89 34.649175006762235 37.0 33.0 37.0 33.0 37.0 90-91 34.10630240735732 37.0 33.0 37.0 30.0 37.0 92-93 33.99594265620773 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 6.0 22 3.0 23 8.0 24 9.0 25 18.0 26 21.0 27 21.0 28 32.0 29 36.0 30 39.0 31 87.0 32 81.0 33 115.0 34 170.0 35 354.0 36 873.0 37 1053.0 38 1022.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 82.92500000000001 4.075 4.55 8.450000000000001 2 60.575 22.975 11.0 5.45 3 33.5 38.125 16.2 12.174999999999999 4 33.725 26.474999999999998 21.25 18.55 5 21.525 32.05 27.6 18.825 6 20.424999999999997 37.3 27.224999999999998 15.049999999999999 7 35.099999999999994 30.025000000000002 20.8 14.075 8 31.0 29.15 24.3 15.55 9 26.35 27.35 27.375 18.925 10-11 24.25 28.3875 28.6875 18.675 12-13 27.5125 25.362499999999997 29.225 17.9 14-15 22.400000000000002 26.1625 31.3125 20.125 16-17 24.9125 30.349999999999998 27.5875 17.150000000000002 18-19 23.5125 26.3625 27.700000000000003 22.425 20-21 25.6125 25.374999999999996 28.4125 20.599999999999998 22-23 26.150000000000002 24.212500000000002 28.4125 21.224999999999998 24-25 25.650000000000002 24.575 29.4125 20.3625 26-27 24.9 25.074999999999996 29.525000000000002 20.5 28-29 25.525 26.174999999999997 28.425 19.875 30-31 26.700000000000003 25.6125 28.5875 19.1 32-33 23.6875 27.5875 28.8875 19.8375 34-35 24.5625 25.275 29.275000000000002 20.8875 36-37 24.05 25.5625 29.325000000000003 21.0625 38-39 24.8 25.45 30.2375 19.5125 40-41 25.84073009126141 24.478059757469683 29.828728591073883 19.852481560195024 42-43 23.6625 27.35 29.099999999999998 19.8875 44-45 22.375 26.575 30.2125 20.837500000000002 46-47 24.3 25.337500000000002 29.2375 21.125 48-49 24.2375 25.0625 31.474999999999998 19.225 50-51 24.85 25.6 29.2375 20.3125 52-53 24.85 26.3 28.1375 20.7125 54-55 23.474999999999998 27.925 29.099999999999998 19.5 56-57 23.45 26.275 29.9 20.375 58-59 23.6875 26.6 28.475 21.2375 60-61 24.775 25.1 30.175 19.950000000000003 62-63 22.625 27.55 30.9375 18.8875 64-65 23.6625 26.7125 29.625 20.0 66-67 23.799999999999997 27.9375 29.725 18.5375 68-69 23.825 26.275 29.562500000000004 20.3375 70-71 23.477825106489604 26.033575544976195 30.31821598596843 20.170383362565772 72-73 25.145165362282253 25.0189346124716 29.89144155516284 19.944458470083312 74-75 23.206535613990297 25.619096247127903 30.776104161347973 20.398263977533826 76-77 21.725692208628463 26.722472633612366 31.963940759819703 19.587894397939472 78-79 23.75439052946533 25.70573695850137 30.727201769220763 19.81267074281254 80-81 23.43811653294752 27.44969091148231 30.922004471918978 18.19018808365119 82-83 23.794340374651256 25.734024179620036 30.476949647934106 19.994685797794606 84-85 23.26051779935275 25.01348435814455 31.58036677454153 20.145631067961165 86-87 23.032188260751962 26.548552880714094 31.07925344874222 19.340005409791722 88-89 22.88341898836895 28.320259670002706 30.619421152285636 18.17690018934271 90-91 23.721936705436843 27.035434135785774 30.29483364890452 18.94779550987287 92-93 22.044901271301054 29.632134162834735 29.80795239383284 18.51501217203138 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 2.0 18 2.5 19 1.0 20 1.0 21 2.5 22 3.5 23 4.5 24 6.5 25 8.0 26 11.5 27 12.5 28 17.5 29 24.5 30 26.5 31 41.5 32 62.5 33 75.5 34 96.5 35 135.0 36 153.5 37 174.0 38 205.0 39 189.5 40 183.0 41 202.5 42 198.5 43 191.0 44 202.5 45 203.0 46 208.0 47 207.5 48 178.0 49 160.0 50 144.0 51 138.0 52 142.5 53 129.0 54 104.5 55 80.0 56 52.0 57 36.0 58 39.5 59 43.5 60 40.5 61 37.0 62 33.5 63 26.0 64 18.5 65 11.5 66 16.5 67 15.5 68 6.0 69 6.0 70 4.0 71 2.0 72 1.0 73 0.5 74 1.5 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0125 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 18.0 71 8.0 72 26.0 73 21.0 74 20.0 75 15.0 76 19.0 77 21.0 78 17.0 79 26.0 80 15.0 81 18.0 82 25.0 83 32.0 84 22.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3697.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.125 #Duplication Level Percentage of deduplicated Percentage of total 1 94.97872340425532 83.7 2 3.0921985815602837 5.45 3 0.7943262411347518 2.1 4 0.3971631205673759 1.4000000000000001 5 0.19858156028368795 0.8750000000000001 6 0.05673758865248227 0.3 7 0.0851063829787234 0.525 8 0.028368794326241134 0.2 9 0.028368794326241134 0.22499999999999998 >10 0.3404255319148936 5.225 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 29 0.7250000000000001 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 28 0.7000000000000001 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 23 0.575 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 19 0.475 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 19 0.475 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 17 0.42500000000000004 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 16 0.4 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 14 0.35000000000000003 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 13 0.325 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 11 0.27499999999999997 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 10 0.25 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 10 0.25 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 9 0.22499999999999998 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 8 0.2 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 7 0.17500000000000002 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 7 0.17500000000000002 No Hit TCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATCT 7 0.17500000000000002 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 6 0.15 No Hit GGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTAATAG 6 0.15 No Hit GGGCCTGTTATCTCTATCAATATGATTCTAATTCGTCAGATATTATTTAT 5 0.125 No Hit GGGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGA 5 0.125 No Hit GAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTAATAGA 5 0.125 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 5 0.125 No Hit GGAGGAGATCGAGTTGTTACTTGAGAGTTTGTAACCCTTTATCATGCCAT 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.037500000000000006 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.07500000000000001 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209034 READS because READLEN < 1 Read 209034 spots for ERR6133382.sra Written 209034 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra Rejected 209033 READS because READLEN < 1 Read 209033 spots for ERR6133382.sra Written 209033 spots for ERR6133382.sra SRR ids: ['ERR6133382.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7rh7r6qk ERR6133382.sra spots: 4180661 blocks: [[1, 209033], [209034, 418066], [418067, 627099], [627100, 836132], [836133, 1045165], [1045166, 1254198], [1254199, 1463231], [1463232, 1672264], [1672265, 1881297], [1881298, 2090330], [2090331, 2299363], [2299364, 2508396], [2508397, 2717429], [2717430, 2926462], [2926463, 3135495], [3135496, 3344528], [3344529, 3553561], [3553562, 3762594], [3762595, 3971627], [3971628, 4180661]] ERR6133382 file size 918215 ERR6133382 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133382 ERR6133382_1.fastq Input file: ERR6133382_1.fastq trimmed: ERR6133382-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:20:05 2024 >> started Sat Dec 7 02:20:07 2024 >> done (2.445s) 4180661 reads processed; of these: 619 ( 0.01%) short reads filtered out after trimming by size control 19 ( 0.00%) empty reads filtered out after trimming by size control 4180023 (99.98%) reads available; of these: 155746 ( 3.73%) trimmed reads available after processing 4024277 (96.27%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 74 0.00% 19 126 0.00% 20 37 0.00% 21 41 0.00% 22 45 0.00% 23 18 0.00% 24 34 0.00% 25 17 0.00% 26 37 0.00% 27 13 0.00% 28 29 0.00% 29 45 0.00% 30 24 0.00% 31 34 0.00% 32 83 0.00% 33 23 0.00% 34 19 0.00% 35 27 0.00% 36 24 0.00% 37 30 0.00% 38 78 0.00% 39 159 0.00% 40 371 0.01% 41 57 0.00% 42 36 0.00% 43 36 0.00% 44 49 0.00% 45 36 0.00% 46 84 0.00% 47 151 0.00% 48 243 0.01% 49 1428 0.03% 50 386 0.01% 51 516 0.01% 52 384 0.01% 53 507 0.01% 54 583 0.01% 55 609 0.01% 56 665 0.02% 57 884 0.02% 58 800 0.02% 59 953 0.02% 60 1093 0.03% 61 866 0.02% 62 987 0.02% 63 1217 0.03% 64 1174 0.03% 65 1112 0.03% 66 1307 0.03% 67 1422 0.03% 68 1743 0.04% 69 1081 0.03% 70 22272 0.53% 71 22004 0.53% 72 26465 0.63% 73 23225 0.56% 74 24010 0.57% 75 25989 0.62% 76 22455 0.54% 77 22639 0.54% 78 25395 0.61% 79 26590 0.64% 80 24019 0.57% 81 23838 0.57% 82 27167 0.65% 83 32574 0.78% 84 25724 0.62% 85 6120 0.15% 86 7028 0.17% 87 8070 0.19% 88 10479 0.25% 89 10278 0.25% 90 13518 0.32% 91 15107 0.36% 92 17490 0.42% 93 3695770 88.42% 4180023 reads passed initial QC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=6.62 fanout-score-rank=23 prefix-density=0.62 prefix-fanout=4.4 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=104.88 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=6.2 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTATAGGGAAAACATAATTTATGAGACAATCATACTGTGCAACTCTTGGCTCCATCGATTAATTAATACTCCTTGTTATTGCTTGCATGGTGG Started job on | Dec 07 02:20:24 Started mapping on | Dec 07 02:20:24 Finished on | Dec 07 02:20:33 Mapping speed, Million of reads per hour | 1672.01 Number of input reads | 4180023 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 3260157 Uniquely mapped reads % | 77.99% Average mapped length | 90.71 Number of splices: Total | 123727 Number of splices: Annotated (sjdb) | 100780 Number of splices: GT/AG | 115780 Number of splices: GC/AG | 3996 Number of splices: AT/AC | 49 Number of splices: Non-canonical | 3902 Mismatch rate per base, % | 0.59% Deletion rate per base | 0.05% Deletion average length | 1.84 Insertion rate per base | 0.03% Insertion average length | 1.73 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 747644 % of reads mapped to multiple loci | 17.89% Number of reads mapped to too many loci | 42217 % of reads mapped to too many loci | 1.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.99% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 172222 172222 172222 N_multimapping 747644 747644 747644 N_noFeature 172155 201348 3102669 N_ambiguous 139874 11746 389 UnstrandedReadsAssigned:2948128 PositiveStrandReadsAssigned:3047063 NegativeStrandReadsAssigned:157099 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133382 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133382-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 4,180,023 reads, 3,480,905 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,083 rounds 52973 ERR6133382.ke.tsv 35125 ERR6133382.se.tsv 88098 total ==> ERR6133382.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 83 24.396 PNS24243 293 194 0 0 KQK14069 1603 1504 38 10.189 KQK14071 474 375 0 0 ==> ERR6133382.se.tsv <== BRADI_1g14170v3 38 BRADI_1g53295v3 106 BRADI_1g59795v3 58 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 55 BRADI_1g74790v3 19 BRADI_1g09890v3 0 BRADI_1g77505v3 79 BRADI_1g48960v3 0 ERR6133382 completed mapping pipeline successfully