Starting /dee2/code/volunteer_pipeline.sh ERR6133383 current disk space = 1547620118528 free memory = 1491182488 ERR6133383 SRAfilesize 851e7b263f6bbd85a39c0e3dd514558e ERR6133383.sra ERR6133383.sra file validated ERR6133383 is single end ERR6133383 is conventional basespace ERR6133383 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133383_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.88125 37.0 37.0 37.0 37.0 37.0 2 36.7485 37.0 37.0 37.0 37.0 37.0 3 36.61075 37.0 37.0 37.0 37.0 37.0 4 36.14225 37.0 37.0 37.0 33.0 37.0 5 36.1405 37.0 37.0 37.0 33.0 37.0 6 36.249 37.0 37.0 37.0 37.0 37.0 7 38.2025 40.0 37.0 40.0 37.0 40.0 8 38.22175 40.0 37.0 40.0 37.0 40.0 9 38.2355 40.0 37.0 40.0 37.0 40.0 10-11 38.185249999999996 40.0 37.0 40.0 37.0 40.0 12-13 38.189499999999995 40.0 37.0 40.0 37.0 40.0 14-15 38.145624999999995 40.0 37.0 40.0 37.0 40.0 16-17 38.05625 40.0 37.0 40.0 35.0 40.0 18-19 38.001999999999995 40.0 37.0 40.0 33.0 40.0 20-21 37.877875 40.0 37.0 40.0 33.0 40.0 22-23 37.797375 40.0 37.0 40.0 33.0 40.0 24-25 37.6445 40.0 37.0 40.0 33.0 40.0 26-27 37.541624999999996 40.0 37.0 40.0 33.0 40.0 28-29 37.40025 37.0 37.0 40.0 33.0 40.0 30-31 37.29175 37.0 37.0 40.0 33.0 40.0 32-33 37.17275 37.0 37.0 40.0 33.0 40.0 34-35 36.931375 37.0 37.0 40.0 33.0 40.0 36-37 36.967749999999995 37.0 37.0 40.0 33.0 40.0 38-39 37.013 37.0 37.0 40.0 33.0 40.0 40-41 37.246375 37.0 37.0 40.0 33.0 40.0 42-43 37.274125 37.0 37.0 40.0 33.0 40.0 44-45 37.153875 37.0 37.0 40.0 33.0 40.0 46-47 36.855999999999995 37.0 37.0 40.0 33.0 40.0 48-49 36.82425 37.0 37.0 40.0 33.0 40.0 50-51 36.727125 37.0 37.0 40.0 33.0 40.0 52-53 36.473625 37.0 37.0 38.5 33.0 40.0 54-55 36.364625000000004 37.0 37.0 37.0 33.0 40.0 56-57 36.16875 37.0 37.0 37.0 33.0 40.0 58-59 36.025375 37.0 37.0 37.0 33.0 40.0 60-61 35.902249999999995 37.0 37.0 37.0 33.0 40.0 62-63 35.602000000000004 37.0 35.0 37.0 33.0 37.0 64-65 35.376125 37.0 33.0 37.0 33.0 37.0 66-67 35.287125 37.0 33.0 37.0 33.0 37.0 68-69 34.470625 35.0 33.0 37.0 30.0 37.0 70-71 34.61594477911647 37.0 33.0 37.0 33.0 37.0 72-73 35.024113754063634 37.0 33.0 37.0 33.0 37.0 74-75 34.96958898399451 37.0 33.0 37.0 33.0 37.0 76-77 34.852332506491344 37.0 33.0 37.0 33.0 37.0 78-79 34.98833316135792 37.0 33.0 37.0 33.0 37.0 80-81 34.830030285319594 37.0 33.0 37.0 33.0 37.0 82-83 34.50268780687546 37.0 33.0 37.0 33.0 37.0 84-85 34.52600263477714 37.0 33.0 37.0 33.0 37.0 86-87 34.5725914064585 37.0 33.0 37.0 33.0 37.0 88-89 34.47304510274887 37.0 33.0 37.0 33.0 37.0 90-91 33.985988791032824 37.0 33.0 37.0 30.0 37.0 92-93 33.816920202828925 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 5.0 22 6.0 23 14.0 24 13.0 25 11.0 26 23.0 27 25.0 28 31.0 29 53.0 30 49.0 31 56.0 32 76.0 33 121.0 34 198.0 35 362.0 36 834.0 37 1045.0 38 1026.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 81.25 4.6 4.7 9.45 2 56.95 24.349999999999998 12.25 6.45 3 33.375 35.949999999999996 16.575 14.099999999999998 4 33.525 25.025 20.075000000000003 21.375 5 22.0 31.424999999999997 27.275 19.3 6 20.549999999999997 36.775000000000006 27.275 15.4 7 34.075 29.725 21.05 15.15 8 29.475 29.925 24.15 16.45 9 26.25 26.200000000000003 28.425 19.125 10-11 24.775 27.712500000000002 28.6875 18.825 12-13 26.9125 25.7125 29.9375 17.4375 14-15 21.75 26.174999999999997 32.2 19.875 16-17 24.8625 30.525000000000002 27.150000000000002 17.4625 18-19 23.65 25.825 27.8375 22.6875 20-21 25.4375 26.55 27.85 20.1625 22-23 25.8625 24.275 29.45 20.4125 24-25 25.662499999999998 24.962500000000002 28.787499999999998 20.5875 26-27 25.7875 24.575 30.012499999999996 19.625 28-29 25.3 26.7125 27.5625 20.424999999999997 30-31 26.2125 25.025 28.725 20.0375 32-33 23.0 27.175 29.049999999999997 20.775 34-35 25.6125 24.5 29.3375 20.549999999999997 36-37 25.0375 24.1125 28.799999999999997 22.05 38-39 25.25 24.5125 30.95 19.287499999999998 40-41 24.9875 25.637500000000003 28.199999999999996 21.175 42-43 24.55 28.1375 28.125 19.1875 44-45 22.85 26.3125 29.7875 21.05 46-47 24.212500000000002 23.7 29.862499999999997 22.225 48-49 24.8625 25.587500000000002 29.9 19.650000000000002 50-51 24.099999999999998 25.35 29.75 20.8 52-53 24.337500000000002 27.3625 28.849999999999998 19.45 54-55 23.674999999999997 26.9625 29.599999999999998 19.7625 56-57 24.1625 26.1625 29.925 19.75 58-59 23.799999999999997 25.0625 30.225 20.9125 60-61 24.75 25.174999999999997 29.9 20.175 62-63 22.5875 28.0625 30.349999999999998 19.0 64-65 23.6625 26.35 29.849999999999998 20.1375 66-67 23.525 27.900000000000002 30.1375 18.4375 68-69 24.3 26.625 29.325000000000003 19.75 70-71 24.511523046092183 25.062625250501004 29.283567134268534 21.142284569138276 72-73 26.04469132685267 24.857972478222447 29.88259058199722 19.21474561292766 74-75 23.03478150082813 25.595617276086124 31.532679322206654 19.836921900879094 76-77 22.477535301668805 25.89216944801027 31.964056482670088 19.666238767650835 78-79 24.754013464526153 25.479026411185913 29.609010875194198 20.157949249093736 80-81 23.176301709513243 27.665405193788335 30.471094871460263 18.68719822523816 82-83 23.6540739765697 25.102013952876135 31.104383309201 20.139528761353166 84-85 22.9765708200213 24.48083067092652 31.922257720979765 20.620340788072415 86-87 23.2986389111289 26.114224713103816 30.491059514278092 20.09607686148919 88-89 22.177742193755005 28.863090472377902 30.544435548438752 18.414731785428344 90-91 23.832399252735524 27.768881772084335 29.063250600480384 19.33546837469976 92-93 22.431278356018147 29.39685081398452 29.370162796904193 18.801708033093142 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 5.5 18 6.0 19 1.5 20 1.0 21 2.0 22 2.0 23 2.5 24 4.5 25 6.5 26 11.0 27 15.0 28 23.0 29 28.5 30 28.5 31 37.5 32 60.0 33 72.0 34 88.0 35 104.0 36 132.0 37 177.0 38 185.0 39 193.0 40 215.0 41 216.5 42 202.5 43 192.5 44 198.0 45 191.5 46 193.0 47 202.0 48 173.0 49 163.5 50 168.0 51 154.5 52 136.5 53 128.5 54 102.5 55 59.5 56 50.0 57 53.5 58 59.5 59 49.5 60 39.0 61 35.0 62 24.5 63 27.5 64 25.5 65 20.5 66 22.0 67 15.5 68 7.5 69 6.5 70 6.0 71 5.0 72 3.5 73 1.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 16.0 71 15.0 72 17.0 73 17.0 74 21.0 75 12.0 76 14.0 77 15.0 78 22.0 79 16.0 80 7.0 81 19.0 82 21.0 83 23.0 84 18.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3747.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.6 #Duplication Level Percentage of deduplicated Percentage of total 1 95.54794520547945 83.7 2 2.711187214611872 4.75 3 0.5707762557077625 1.5 4 0.228310502283105 0.8 5 0.2568493150684931 1.125 6 0.05707762557077625 0.3 7 0.1141552511415525 0.7000000000000001 8 0.028538812785388126 0.2 9 0.08561643835616438 0.675 >10 0.3995433789954338 6.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 37 0.9249999999999999 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 30 0.75 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 26 0.65 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 22 0.5499999999999999 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 20 0.5 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 17 0.42500000000000004 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 16 0.4 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 16 0.4 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 14 0.35000000000000003 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 12 0.3 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 10 0.25 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 10 0.25 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 10 0.25 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 10 0.25 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 9 0.22499999999999998 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 9 0.22499999999999998 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 9 0.22499999999999998 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 8 0.2 No Hit GGGCCTGTTATCTCTATCAATATGATTCTAATTCGTCAGATATTATTTAT 7 0.17500000000000002 No Hit GGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAG 7 0.17500000000000002 No Hit GTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCA 7 0.17500000000000002 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 7 0.17500000000000002 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 6 0.15 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 6 0.15 No Hit GGGCCAGGCCGTGGGTATACGAGGTCTCTGTACATGTGTTTAATCTGCAG 5 0.125 No Hit GGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTC 5 0.125 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 5 0.125 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 5 0.125 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 5 0.125 No Hit GCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGA 5 0.125 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 5 0.125 No Hit GGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAGGCAAA 5 0.125 No Hit GGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGCG 20 0.0028448023 64.575 1 >>END_MODULE Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra Rejected 154912 READS because READLEN < 1 Read 154912 spots for ERR6133383.sra Written 154912 spots for ERR6133383.sra SRR ids: ['ERR6133383.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zzaoodgw ERR6133383.sra spots: 3098240 blocks: [[1, 154912], [154913, 309824], [309825, 464736], [464737, 619648], [619649, 774560], [774561, 929472], [929473, 1084384], [1084385, 1239296], [1239297, 1394208], [1394209, 1549120], [1549121, 1704032], [1704033, 1858944], [1858945, 2013856], [2013857, 2168768], [2168769, 2323680], [2323681, 2478592], [2478593, 2633504], [2633505, 2788416], [2788417, 2943328], [2943329, 3098240]] ERR6133383 file size 680734 ERR6133383 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133383 ERR6133383_1.fastq Input file: ERR6133383_1.fastq trimmed: ERR6133383-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:31:10 2024 >> started Sat Dec 7 02:31:15 2024 >> done (5.000s) 3098240 reads processed; of these: 365 ( 0.01%) short reads filtered out after trimming by size control 12 ( 0.00%) empty reads filtered out after trimming by size control 3097863 (99.99%) reads available; of these: 117496 ( 3.79%) trimmed reads available after processing 2980367 (96.21%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 31 0.00% 19 49 0.00% 20 17 0.00% 21 17 0.00% 22 18 0.00% 23 11 0.00% 24 11 0.00% 25 12 0.00% 26 16 0.00% 27 17 0.00% 28 19 0.00% 29 94 0.00% 30 10 0.00% 31 21 0.00% 32 26 0.00% 33 11 0.00% 34 16 0.00% 35 9 0.00% 36 7 0.00% 37 18 0.00% 38 36 0.00% 39 53 0.00% 40 127 0.00% 41 21 0.00% 42 20 0.00% 43 14 0.00% 44 28 0.00% 45 38 0.00% 46 54 0.00% 47 102 0.00% 48 159 0.01% 49 1035 0.03% 50 264 0.01% 51 310 0.01% 52 337 0.01% 53 323 0.01% 54 377 0.01% 55 441 0.01% 56 494 0.02% 57 633 0.02% 58 564 0.02% 59 669 0.02% 60 878 0.03% 61 697 0.02% 62 728 0.02% 63 899 0.03% 64 916 0.03% 65 876 0.03% 66 1021 0.03% 67 1165 0.04% 68 1428 0.05% 69 778 0.03% 70 14548 0.47% 71 14938 0.48% 72 17871 0.58% 73 15327 0.49% 74 15679 0.51% 75 16913 0.55% 76 14836 0.48% 77 14841 0.48% 78 17431 0.56% 79 18191 0.59% 80 16344 0.53% 81 16361 0.53% 82 18759 0.61% 83 22789 0.74% 84 18318 0.59% 85 4549 0.15% 86 5376 0.17% 87 6398 0.21% 88 7878 0.25% 89 7776 0.25% 90 10626 0.34% 91 11907 0.38% 92 12950 0.42% 93 2761342 89.14% 3097863 reads passed initial QC criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=5.74 fanout-score-rank=28 prefix-density=0.29 prefix-fanout=4.3 sequence=GTGTTGTGTTGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=83.85 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=7.6 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTATAGGGAAAACATAATTTATGAGACAATCATACTGTGCAACTCTTGGCTCCATCGATTAATTAATACTCCTTGTTATTGCTTGCATGGTGG Started job on | Dec 07 02:31:36 Started mapping on | Dec 07 02:31:37 Finished on | Dec 07 02:32:07 Mapping speed, Million of reads per hour | 371.74 Number of input reads | 3097863 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 2418398 Uniquely mapped reads % | 78.07% Average mapped length | 91.09 Number of splices: Total | 101182 Number of splices: Annotated (sjdb) | 85913 Number of splices: GT/AG | 97194 Number of splices: GC/AG | 2458 Number of splices: AT/AC | 33 Number of splices: Non-canonical | 1497 Mismatch rate per base, % | 0.32% Deletion rate per base | 0.02% Deletion average length | 1.43 Insertion rate per base | 0.00% Insertion average length | 1.27 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 570398 % of reads mapped to multiple loci | 18.41% Number of reads mapped to too many loci | 33668 % of reads mapped to too many loci | 1.09% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.29% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 109067 109067 109067 N_multimapping 570398 570398 570398 N_noFeature 118347 141425 2303816 N_ambiguous 99932 8459 276 UnstrandedReadsAssigned:2200119 PositiveStrandReadsAssigned:2268514 NegativeStrandReadsAssigned:114306 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133383 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133383-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,097,863 reads, 2,633,709 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,024 rounds 52973 ERR6133383.ke.tsv 35125 ERR6133383.se.tsv 88098 total ==> ERR6133383.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 4.00219 1.1714 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 49.9978 19.5082 PNS24243 293 194 0 0 KQK14069 1603 1504 203 72.2551 KQK14071 474 375 0 0 ==> ERR6133383.se.tsv <== BRADI_1g14170v3 204 BRADI_1g53295v3 87 BRADI_1g59795v3 27 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 25 BRADI_1g74790v3 32 BRADI_1g09890v3 0 BRADI_1g77505v3 70 BRADI_1g48960v3 0 ERR6133383 completed mapping pipeline successfully