Starting /dee2/code/volunteer_pipeline.sh ERR6133384 current disk space = 1547620118528 free memory = 1600324096 ERR6133384 SRAfilesize 7550de00fc58ddc1ec57397223b78da3 ERR6133384.sra ERR6133384.sra file validated ERR6133384 is single end ERR6133384 is conventional basespace ERR6133384 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133384_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.883 37.0 37.0 37.0 37.0 37.0 2 36.7605 37.0 37.0 37.0 37.0 37.0 3 36.60825 37.0 37.0 37.0 37.0 37.0 4 36.06625 37.0 37.0 37.0 33.0 37.0 5 36.0605 37.0 37.0 37.0 33.0 37.0 6 36.27375 37.0 37.0 37.0 33.0 37.0 7 38.21375 40.0 37.0 40.0 37.0 40.0 8 38.286 40.0 37.0 40.0 37.0 40.0 9 38.2125 40.0 37.0 40.0 37.0 40.0 10-11 38.201750000000004 40.0 37.0 40.0 37.0 40.0 12-13 38.227625 40.0 37.0 40.0 37.0 40.0 14-15 38.15625 40.0 37.0 40.0 37.0 40.0 16-17 38.049125000000004 40.0 37.0 40.0 35.0 40.0 18-19 38.0105 40.0 37.0 40.0 37.0 40.0 20-21 37.8995 40.0 37.0 40.0 33.0 40.0 22-23 37.889875 40.0 37.0 40.0 33.0 40.0 24-25 37.7595 40.0 37.0 40.0 33.0 40.0 26-27 37.6605 40.0 37.0 40.0 33.0 40.0 28-29 37.502750000000006 40.0 37.0 40.0 33.0 40.0 30-31 37.438625 37.0 37.0 40.0 33.0 40.0 32-33 37.197500000000005 37.0 37.0 40.0 33.0 40.0 34-35 37.016125 37.0 37.0 40.0 33.0 40.0 36-37 37.002250000000004 37.0 37.0 40.0 33.0 40.0 38-39 36.99975 37.0 37.0 40.0 33.0 40.0 40-41 37.23675 37.0 37.0 40.0 33.0 40.0 42-43 37.283375 37.0 37.0 40.0 33.0 40.0 44-45 37.195375 37.0 37.0 40.0 33.0 40.0 46-47 36.965 37.0 37.0 40.0 33.0 40.0 48-49 36.836625 37.0 37.0 40.0 33.0 40.0 50-51 36.717 37.0 37.0 40.0 33.0 40.0 52-53 36.53575 37.0 37.0 38.5 33.0 40.0 54-55 36.394125 37.0 37.0 37.0 33.0 40.0 56-57 36.118625 37.0 37.0 37.0 33.0 40.0 58-59 36.035375 37.0 37.0 37.0 33.0 40.0 60-61 35.806875000000005 37.0 37.0 37.0 33.0 40.0 62-63 35.573499999999996 37.0 35.0 37.0 33.0 37.0 64-65 35.346000000000004 37.0 33.0 37.0 33.0 37.0 66-67 35.327749999999995 37.0 33.0 37.0 33.0 37.0 68-69 34.443375 35.0 33.0 37.0 30.0 37.0 70-71 34.61647078736209 37.0 33.0 37.0 33.0 37.0 72-73 35.02378456315832 37.0 33.0 37.0 33.0 37.0 74-75 35.06108340054013 37.0 33.0 37.0 33.0 37.0 76-77 34.90404154268967 37.0 33.0 37.0 33.0 37.0 78-79 34.96412453993141 37.0 33.0 37.0 33.0 37.0 80-81 34.91029802416716 37.0 33.0 37.0 33.0 37.0 82-83 34.64170208844071 37.0 33.0 37.0 33.0 37.0 84-85 34.67747539619188 37.0 33.0 37.0 33.0 37.0 86-87 34.64917421417155 37.0 33.0 37.0 33.0 37.0 88-89 34.51172083111348 37.0 33.0 37.0 33.0 37.0 90-91 34.108950452850294 37.0 33.0 37.0 27.0 37.0 92-93 33.93939797549281 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 7.0 21 6.0 22 6.0 23 11.0 24 12.0 25 13.0 26 12.0 27 21.0 28 25.0 29 42.0 30 41.0 31 77.0 32 95.0 33 126.0 34 189.0 35 376.0 36 822.0 37 1046.0 38 1020.0 39 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 78.375 5.525 5.55 10.549999999999999 2 56.75 25.424999999999997 11.675 6.15 3 33.575 35.075 17.325 14.025000000000002 4 32.15 25.674999999999997 21.125 21.05 5 21.975 32.025 27.025 18.975 6 21.65 37.25 26.275 14.825 7 32.6 28.749999999999996 22.55 16.1 8 31.900000000000002 27.450000000000003 23.400000000000002 17.25 9 24.675 26.650000000000002 29.099999999999998 19.575 10-11 24.2375 28.000000000000004 28.575 19.1875 12-13 27.224999999999998 24.349999999999998 30.0875 18.337500000000002 14-15 22.35 25.55 32.25 19.85 16-17 25.324999999999996 30.825000000000003 26.9625 16.8875 18-19 24.075 25.5625 28.0625 22.3 20-21 25.074999999999996 26.3625 28.375 20.1875 22-23 26.0125 23.9375 28.512500000000003 21.5375 24-25 24.1125 25.275 29.812499999999996 20.8 26-27 25.650000000000002 24.7375 30.2875 19.325 28-29 24.2875 25.7625 28.575 21.375 30-31 25.7125 24.55 29.5875 20.150000000000002 32-33 23.6125 26.950000000000003 29.7875 19.650000000000002 34-35 24.2625 24.9375 29.2375 21.5625 36-37 23.9875 24.625 29.9 21.4875 38-39 24.8625 25.4 30.099999999999998 19.6375 40-41 25.5125 25.4 28.199999999999996 20.8875 42-43 23.6375 28.0625 27.775 20.525 44-45 22.675 25.7 31.05 20.575 46-47 24.0625 25.75 28.512500000000003 21.675 48-49 23.674999999999997 26.174999999999997 30.099999999999998 20.05 50-51 23.9 25.2 30.337500000000002 20.5625 52-53 24.95 26.387500000000003 29.212500000000002 19.45 54-55 24.55 26.575 28.8625 20.0125 56-57 24.875 26.3 29.912499999999998 18.912499999999998 58-59 23.4625 25.55 30.599999999999998 20.3875 60-61 23.7875 25.9875 30.575000000000003 19.650000000000002 62-63 22.375 27.6875 30.6375 19.3 64-65 23.225 26.275 30.775000000000002 19.725 66-67 24.525 26.900000000000002 29.1625 19.412499999999998 68-69 23.2375 26.087500000000002 29.1125 21.5625 70-71 23.635453179769655 25.0 30.908863294942414 20.45568352528793 72-73 24.940138626339003 25.444234404536864 28.8468809073724 20.768746061751735 74-75 22.66717518433766 26.44291889143148 30.638189677091276 20.251716247139587 76-77 23.4328932188181 24.804512242020255 31.0985771054993 20.66401743366235 78-79 24.81582008530438 25.552539744086854 30.48985394855887 19.141786222049888 80-81 23.850462420216232 26.338413442751076 30.272241761104596 19.538882375928097 82-83 23.459061637534496 25.824681298462348 31.39702983309239 19.319227230910762 84-85 22.756878904692275 24.325402100225972 31.70277814701582 21.21494084806593 86-87 22.948854555141182 26.704848161960577 30.2477357485349 20.098561534363345 88-89 23.028769312733086 28.223228556206713 29.768247202983485 18.979754928076716 90-91 22.802344166222696 27.690463505594032 29.39531166755461 20.11188066062866 92-93 22.762386787426745 29.062333510921682 29.63505594033031 18.540223761321258 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 4.0 18 4.0 19 0.5 20 0.0 21 1.0 22 2.5 23 5.0 24 6.5 25 8.5 26 10.0 27 16.5 28 25.5 29 29.0 30 35.5 31 43.5 32 60.5 33 80.5 34 91.5 35 112.5 36 132.0 37 159.5 38 179.5 39 179.5 40 193.0 41 205.0 42 215.5 43 224.5 44 210.0 45 193.0 46 205.0 47 199.0 48 172.0 49 167.0 50 175.5 51 161.0 52 127.5 53 110.5 54 86.0 55 62.5 56 54.0 57 49.0 58 56.0 59 55.5 60 41.5 61 30.0 62 32.0 63 28.0 64 22.5 65 24.5 66 19.0 67 17.5 68 14.0 69 5.5 70 2.5 71 3.0 72 2.5 73 2.0 74 1.5 75 1.0 76 0.5 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 12.0 71 11.0 72 19.0 73 17.0 74 16.0 75 17.0 76 15.0 77 13.0 78 23.0 79 12.0 80 13.0 81 19.0 82 17.0 83 27.0 84 15.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3754.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.575 #Duplication Level Percentage of deduplicated Percentage of total 1 95.28648038385549 84.39999999999999 2 3.132938187976291 5.55 3 0.5644933672029353 1.5 4 0.1693480101608806 0.6 5 0.1693480101608806 0.75 6 0.11289867344058707 0.6 7 0.11289867344058707 0.7000000000000001 8 0.0846740050804403 0.6 9 0.028224668360146768 0.22499999999999998 >10 0.3386960203217612 5.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 40 1.0 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 21 0.525 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 18 0.44999999999999996 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 17 0.42500000000000004 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 17 0.42500000000000004 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 16 0.4 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 14 0.35000000000000003 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 14 0.35000000000000003 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 14 0.35000000000000003 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 11 0.27499999999999997 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 11 0.27499999999999997 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 10 0.25 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 9 0.22499999999999998 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 8 0.2 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 8 0.2 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 8 0.2 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 7 0.17500000000000002 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 7 0.17500000000000002 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 7 0.17500000000000002 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 7 0.17500000000000002 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 6 0.15 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 6 0.15 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 6 0.15 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 6 0.15 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 5 0.125 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 5 0.125 No Hit AGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATGAGCCT 5 0.125 No Hit GGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTC 5 0.125 No Hit GGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAG 5 0.125 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.037500000000000006 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129794 READS because READLEN < 1 Read 129794 spots for ERR6133384.sra Written 129794 spots for ERR6133384.sra Rejected 129803 READS because READLEN < 1 Read 129803 spots for ERR6133384.sra Written 129803 spots for ERR6133384.sra SRR ids: ['ERR6133384.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_e16n2sn0 ERR6133384.sra spots: 2595889 blocks: [[1, 129794], [129795, 259588], [259589, 389382], [389383, 519176], [519177, 648970], [648971, 778764], [778765, 908558], [908559, 1038352], [1038353, 1168146], [1168147, 1297940], [1297941, 1427734], [1427735, 1557528], [1557529, 1687322], [1687323, 1817116], [1817117, 1946910], [1946911, 2076704], [2076705, 2206498], [2206499, 2336292], [2336293, 2466086], [2466087, 2595889]] ERR6133384 file size 570311 ERR6133384 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133384 ERR6133384_1.fastq Input file: ERR6133384_1.fastq trimmed: ERR6133384-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:31:03 2024 >> started Sat Dec 7 02:31:05 2024 >> done (1.893s) 2595889 reads processed; of these: 410 ( 0.02%) short reads filtered out after trimming by size control 12 ( 0.00%) empty reads filtered out after trimming by size control 2595467 (99.98%) reads available; of these: 100006 ( 3.85%) trimmed reads available after processing 2495461 (96.15%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 39 0.00% 19 65 0.00% 20 23 0.00% 21 15 0.00% 22 19 0.00% 23 19 0.00% 24 13 0.00% 25 11 0.00% 26 16 0.00% 27 20 0.00% 28 33 0.00% 29 158 0.01% 30 19 0.00% 31 21 0.00% 32 49 0.00% 33 8 0.00% 34 11 0.00% 35 14 0.00% 36 14 0.00% 37 30 0.00% 38 40 0.00% 39 69 0.00% 40 103 0.00% 41 26 0.00% 42 29 0.00% 43 24 0.00% 44 23 0.00% 45 36 0.00% 46 41 0.00% 47 94 0.00% 48 138 0.01% 49 796 0.03% 50 250 0.01% 51 294 0.01% 52 225 0.01% 53 314 0.01% 54 338 0.01% 55 376 0.01% 56 447 0.02% 57 562 0.02% 58 511 0.02% 59 566 0.02% 60 740 0.03% 61 594 0.02% 62 656 0.03% 63 786 0.03% 64 769 0.03% 65 767 0.03% 66 854 0.03% 67 987 0.04% 68 1074 0.04% 69 647 0.02% 70 11938 0.46% 71 11858 0.46% 72 13935 0.54% 73 12382 0.48% 74 12409 0.48% 75 13641 0.53% 76 12345 0.48% 77 12446 0.48% 78 13849 0.53% 79 13897 0.54% 80 13586 0.52% 81 13206 0.51% 82 14591 0.56% 83 17185 0.66% 84 14921 0.57% 85 3915 0.15% 86 4706 0.18% 87 5358 0.21% 88 6706 0.26% 89 6620 0.26% 90 8776 0.34% 91 9826 0.38% 92 11006 0.42% 93 2322592 89.49% 2595467 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=14.08 fanout-score-rank=17 prefix-density=0.51 prefix-fanout=6.3 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=40.40 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=5.1 sequence=TGTATATTATTAAGTAGAAATGACCTTAAGAAAAAACGAACAACAGAATGCAACTGTTGTTTAGTGGTTTGTTGCCTTGTATGTTTTGTTCAGCTCAGCTCAGTGTGTGTGTAGGGAAGATTCCTAAAAAGAAAGGGGGGAGAAGAATGTAGTAGAGTGCACCCTTGTTAATGTGTGTGATGTTCCATGGCAAGCAGCTTAATGTTAATTATTTAGCAGTATGATTGCTGT Started job on | Dec 07 02:31:18 Started mapping on | Dec 07 02:31:19 Finished on | Dec 07 02:31:25 Mapping speed, Million of reads per hour | 1557.28 Number of input reads | 2595467 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 2018583 Uniquely mapped reads % | 77.77% Average mapped length | 90.89 Number of splices: Total | 84877 Number of splices: Annotated (sjdb) | 68698 Number of splices: GT/AG | 78244 Number of splices: GC/AG | 2530 Number of splices: AT/AC | 22 Number of splices: Non-canonical | 4081 Mismatch rate per base, % | 0.59% Deletion rate per base | 0.05% Deletion average length | 1.83 Insertion rate per base | 0.03% Insertion average length | 1.71 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 448807 % of reads mapped to multiple loci | 17.29% Number of reads mapped to too many loci | 27664 % of reads mapped to too many loci | 1.07% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.74% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 128077 128077 128077 N_multimapping 448807 448807 448807 N_noFeature 108213 126826 1924498 N_ambiguous 82122 6723 247 UnstrandedReadsAssigned:1828248 PositiveStrandReadsAssigned:1885034 NegativeStrandReadsAssigned:93838 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133384 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133384-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,595,467 reads, 2,141,553 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 978 rounds 52973 ERR6133384.ke.tsv 35125 ERR6133384.se.tsv 88098 total ==> ERR6133384.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 56 26.7648 PNS24243 293 194 0 0 KQK14069 1603 1504 88 38.3677 KQK14071 474 375 0 0 ==> ERR6133384.se.tsv <== BRADI_1g14170v3 88 BRADI_1g53295v3 70 BRADI_1g59795v3 25 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 41 BRADI_1g74790v3 11 BRADI_1g09890v3 0 BRADI_1g77505v3 47 BRADI_1g48960v3 0 ERR6133384 completed mapping pipeline successfully