Starting /dee2/code/volunteer_pipeline.sh ERR6133385 current disk space = 1547741552640 free memory = 1411439700 ERR6133385 SRAfilesize ec094ff3be8207849890c150d6a397a5 ERR6133385.sra ERR6133385.sra file validated ERR6133385 is single end ERR6133385 is conventional basespace ERR6133385 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133385_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.94875 37.0 37.0 37.0 37.0 37.0 2 36.8175 37.0 37.0 37.0 37.0 37.0 3 36.60975 37.0 37.0 37.0 37.0 37.0 4 36.0765 37.0 37.0 37.0 33.0 37.0 5 36.12525 37.0 37.0 37.0 33.0 37.0 6 36.25575 37.0 37.0 37.0 33.0 37.0 7 38.1945 40.0 37.0 40.0 37.0 40.0 8 38.2395 40.0 37.0 40.0 37.0 40.0 9 38.22425 40.0 37.0 40.0 37.0 40.0 10-11 38.1935 40.0 37.0 40.0 37.0 40.0 12-13 38.192125000000004 40.0 37.0 40.0 37.0 40.0 14-15 38.122249999999994 40.0 37.0 40.0 37.0 40.0 16-17 38.036500000000004 40.0 37.0 40.0 35.0 40.0 18-19 37.98025 40.0 37.0 40.0 33.0 40.0 20-21 37.807 40.0 37.0 40.0 33.0 40.0 22-23 37.765 40.0 37.0 40.0 33.0 40.0 24-25 37.669 40.0 37.0 40.0 33.0 40.0 26-27 37.439875 37.0 37.0 40.0 33.0 40.0 28-29 37.40925 37.0 37.0 40.0 33.0 40.0 30-31 37.3875 37.0 37.0 40.0 33.0 40.0 32-33 37.084875 37.0 37.0 40.0 33.0 40.0 34-35 36.87975 37.0 37.0 40.0 33.0 40.0 36-37 36.88275 37.0 37.0 40.0 33.0 40.0 38-39 37.040625 37.0 37.0 40.0 33.0 40.0 40-41 37.229375000000005 37.0 37.0 40.0 33.0 40.0 42-43 37.154250000000005 37.0 37.0 40.0 33.0 40.0 44-45 37.077625 37.0 37.0 40.0 33.0 40.0 46-47 36.837875 37.0 37.0 40.0 33.0 40.0 48-49 36.770624999999995 37.0 37.0 40.0 33.0 40.0 50-51 36.6665 37.0 37.0 38.5 33.0 40.0 52-53 36.40575 37.0 37.0 37.0 33.0 40.0 54-55 36.283249999999995 37.0 37.0 37.0 33.0 40.0 56-57 36.052125000000004 37.0 37.0 37.0 33.0 40.0 58-59 35.883250000000004 37.0 37.0 37.0 33.0 40.0 60-61 35.748875 37.0 37.0 37.0 33.0 38.5 62-63 35.44775 37.0 33.0 37.0 33.0 37.0 64-65 35.24625 37.0 33.0 37.0 33.0 37.0 66-67 35.24275 37.0 33.0 37.0 33.0 37.0 68-69 34.3475 35.0 33.0 37.0 30.0 37.0 70-71 34.553357572718156 37.0 33.0 37.0 33.0 37.0 72-73 34.88899515882803 37.0 33.0 37.0 33.0 37.0 74-75 34.92076123169488 37.0 33.0 37.0 33.0 37.0 76-77 34.784323912388416 37.0 33.0 37.0 33.0 37.0 78-79 34.94455378760803 37.0 33.0 37.0 33.0 37.0 80-81 34.8759637648392 37.0 33.0 37.0 33.0 37.0 82-83 34.4974388751731 37.0 33.0 37.0 33.0 37.0 84-85 34.425988609719056 37.0 33.0 37.0 33.0 37.0 86-87 34.49064921186214 37.0 33.0 37.0 33.0 37.0 88-89 34.35386053967406 37.0 33.0 37.0 33.0 37.0 90-91 33.983435746727224 37.0 33.0 37.0 27.0 37.0 92-93 33.7675661234304 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 5.0 21 6.0 22 5.0 23 12.0 24 14.0 25 9.0 26 20.0 27 20.0 28 21.0 29 37.0 30 51.0 31 87.0 32 96.0 33 127.0 34 192.0 35 377.0 36 891.0 37 1076.0 38 909.0 39 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 81.875 4.625 4.625 8.875 2 61.475 22.125 10.45 5.949999999999999 3 33.900000000000006 37.625 16.400000000000002 12.075 4 35.225 26.275 18.675 19.825 5 21.95 33.900000000000006 27.200000000000003 16.950000000000003 6 20.549999999999997 37.95 26.8 14.7 7 34.875 29.099999999999998 21.3 14.725 8 29.7 29.099999999999998 24.55 16.650000000000002 9 27.375 25.025 28.7 18.9 10-11 24.2875 28.7375 27.700000000000003 19.275000000000002 12-13 27.3875 24.5375 29.512500000000003 18.5625 14-15 22.112499999999997 26.2875 32.6125 18.987499999999997 16-17 25.8625 30.8125 26.200000000000003 17.125 18-19 23.9875 25.887500000000003 26.737499999999997 23.3875 20-21 26.05 25.937500000000004 27.712500000000002 20.3 22-23 26.637499999999996 23.925 28.262500000000003 21.175 24-25 26.5125 23.6625 29.225 20.599999999999998 26-27 26.1 25.637500000000003 29.312500000000004 18.95 28-29 25.131282820705174 27.206801700425103 28.08202050512628 19.579894973743436 30-31 27.487499999999997 24.7875 27.575 20.150000000000002 32-33 24.7375 28.537499999999998 27.474999999999998 19.25 34-35 24.625 24.962500000000002 29.0875 21.325 36-37 24.712500000000002 24.375 29.475 21.4375 38-39 24.840605075634453 25.203150393799223 31.141392674084262 18.814851856482058 40-41 26.02825353169146 25.21565195649456 27.69096137017127 21.065133141642704 42-43 24.9 28.3375 27.150000000000002 19.6125 44-45 23.2125 26.275 29.599999999999998 20.9125 46-47 23.625 23.6625 29.462500000000002 23.25 48-49 24.4875 25.4375 30.587500000000002 19.4875 50-51 24.5125 25.85 29.275000000000002 20.3625 52-53 24.975 26.575 27.4125 21.0375 54-55 24.05 27.9375 28.4375 19.575 56-57 24.5125 25.6125 29.25 20.625 58-59 23.3625 24.9875 30.325000000000003 21.325 60-61 25.0625 24.462500000000002 29.725 20.75 62-63 22.162499999999998 28.037499999999998 30.8125 18.987499999999997 64-65 24.1125 26.8375 29.4875 19.5625 66-67 24.887500000000003 28.299999999999997 28.1375 18.675 68-69 24.8625 26.85 28.487499999999997 19.8 70-71 25.450676014021035 24.72458688032048 28.818227341011514 21.00650976464697 72-73 25.422659601312137 25.195558920010093 29.182437547312638 20.199343931365128 74-75 23.142493638676847 26.463104325699742 30.419847328244277 19.974554707379134 76-77 21.669238683127574 27.09619341563786 30.465534979423868 20.769032921810698 78-79 23.756476683937823 24.27461139896373 30.544041450777204 21.424870466321245 80-81 24.282359081419624 27.07463465553236 30.114822546972857 18.528183716075155 82-83 23.9752207723738 24.252010017134573 30.93449321207328 20.83827599841835 84-85 24.0064017071219 24.273139503867696 30.554814617231262 21.165644171779142 86-87 22.201442693026983 26.035265829548493 32.01977023777719 19.74352123964734 88-89 21.707186748597383 30.08282126636388 29.695431472081218 18.51456051295752 90-91 24.098316858135185 27.357734437616887 28.85386053967406 19.690088164573872 92-93 22.682340368688216 28.800427464600588 29.201175527651618 19.31605663905958 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 10.0 18 11.5 19 3.0 20 2.0 21 1.0 22 3.0 23 2.5 24 1.0 25 3.0 26 6.5 27 10.0 28 16.0 29 19.5 30 24.0 31 39.0 32 55.0 33 75.5 34 89.5 35 105.5 36 125.0 37 151.5 38 166.5 39 167.5 40 186.5 41 203.0 42 195.5 43 182.0 44 192.5 45 198.5 46 213.5 47 221.5 48 190.5 49 183.5 50 186.0 51 158.5 52 134.0 53 139.5 54 127.5 55 78.5 56 51.5 57 48.5 58 47.5 59 41.0 60 34.0 61 36.5 62 34.5 63 29.0 64 26.0 65 22.5 66 16.0 67 12.5 68 9.5 69 5.0 70 4.0 71 1.5 72 1.5 73 2.5 74 2.5 75 2.0 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.025 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0125 40-41 0.0125 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 12.0 71 16.0 72 18.0 73 18.0 74 12.0 75 29.0 76 14.0 77 16.0 78 10.0 79 11.0 80 24.0 81 17.0 82 19.0 83 29.0 84 12.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3743.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.175 #Duplication Level Percentage of deduplicated Percentage of total 1 94.1088067327923 78.27499999999999 2 3.4565674782085964 5.75 3 0.9017132551848512 2.25 4 0.5410279531109108 1.7999999999999998 5 0.09017132551848511 0.375 6 0.12022843402464682 0.6 7 0.09017132551848511 0.525 8 0.09017132551848511 0.6 9 0.09017132551848511 0.675 >10 0.4809137360985873 7.775 >50 0.030057108506161705 1.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 55 1.375 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 39 0.975 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 30 0.75 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 26 0.65 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 25 0.625 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 24 0.6 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 24 0.6 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 20 0.5 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 20 0.5 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 19 0.475 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 14 0.35000000000000003 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 14 0.35000000000000003 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 14 0.35000000000000003 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 11 0.27499999999999997 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 11 0.27499999999999997 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 10 0.25 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 10 0.25 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 9 0.22499999999999998 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 9 0.22499999999999998 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 9 0.22499999999999998 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 8 0.2 No Hit GCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGA 8 0.2 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 8 0.2 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 7 0.17500000000000002 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 7 0.17500000000000002 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 7 0.17500000000000002 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 6 0.15 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 6 0.15 No Hit GGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAG 6 0.15 No Hit GTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCA 6 0.15 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GAAATCTTTGGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGG 5 0.125 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0125 0.0 0.0 0.0 0.0 12-13 0.037500000000000006 0.0 0.0 0.0 0.0 14-15 0.05 0.0 0.0 0.0 0.0 16-17 0.075 0.0 0.0 0.0 0.0 18-19 0.075 0.0 0.0 0.0 0.0 20-21 0.075 0.0 0.0 0.0 0.0 22-23 0.0875 0.0 0.0 0.0 0.0 24-25 0.1 0.0 0.0 0.0 0.0 26-27 0.1 0.0 0.0 0.0 0.0 28-29 0.1 0.0 0.0 0.0 0.0 30-31 0.1 0.0 0.0 0.0 0.0 32-33 0.1 0.0 0.0 0.0 0.0 34-35 0.1 0.0 0.0 0.0 0.0 36-37 0.1 0.0 0.0 0.0 0.0 38-39 0.1 0.0 0.0 0.0 0.0 40-41 0.1 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119479 READS because READLEN < 1 Read 119479 spots for ERR6133385.sra Written 119479 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra Rejected 119473 READS because READLEN < 1 Read 119473 spots for ERR6133385.sra Written 119473 spots for ERR6133385.sra SRR ids: ['ERR6133385.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_mv88by02 ERR6133385.sra spots: 2389466 blocks: [[1, 119473], [119474, 238946], [238947, 358419], [358420, 477892], [477893, 597365], [597366, 716838], [716839, 836311], [836312, 955784], [955785, 1075257], [1075258, 1194730], [1194731, 1314203], [1314204, 1433676], [1433677, 1553149], [1553150, 1672622], [1672623, 1792095], [1792096, 1911568], [1911569, 2031041], [2031042, 2150514], [2150515, 2269987], [2269988, 2389466]] ERR6133385 file size 524625 ERR6133385 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133385 ERR6133385_1.fastq Input file: ERR6133385_1.fastq trimmed: ERR6133385-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:24:29 2024 >> started Sat Dec 7 02:24:31 2024 >> done (1.823s) 2389466 reads processed; of these: 525 ( 0.02%) short reads filtered out after trimming by size control 7 ( 0.00%) empty reads filtered out after trimming by size control 2388934 (99.98%) reads available; of these: 92809 ( 3.88%) trimmed reads available after processing 2296125 (96.12%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 48 0.00% 19 65 0.00% 20 20 0.00% 21 22 0.00% 22 30 0.00% 23 15 0.00% 24 18 0.00% 25 17 0.00% 26 19 0.00% 27 21 0.00% 28 15 0.00% 29 121 0.01% 30 17 0.00% 31 27 0.00% 32 47 0.00% 33 15 0.00% 34 13 0.00% 35 15 0.00% 36 13 0.00% 37 11 0.00% 38 36 0.00% 39 74 0.00% 40 118 0.00% 41 22 0.00% 42 23 0.00% 43 18 0.00% 44 27 0.00% 45 40 0.00% 46 44 0.00% 47 55 0.00% 48 139 0.01% 49 762 0.03% 50 218 0.01% 51 253 0.01% 52 232 0.01% 53 295 0.01% 54 282 0.01% 55 369 0.02% 56 414 0.02% 57 524 0.02% 58 460 0.02% 59 525 0.02% 60 664 0.03% 61 555 0.02% 62 575 0.02% 63 753 0.03% 64 720 0.03% 65 728 0.03% 66 838 0.04% 67 888 0.04% 68 1163 0.05% 69 576 0.02% 70 11167 0.47% 71 11045 0.46% 72 14281 0.60% 73 11909 0.50% 74 11859 0.50% 75 12954 0.54% 76 10738 0.45% 77 10877 0.46% 78 12778 0.53% 79 13887 0.58% 80 12526 0.52% 81 12104 0.51% 82 13933 0.58% 83 18211 0.76% 84 12930 0.54% 85 3640 0.15% 86 4226 0.18% 87 4897 0.20% 88 6028 0.25% 89 5951 0.25% 90 8294 0.35% 91 9512 0.40% 92 10397 0.44% 93 2131831 89.24% 2388934 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=7.84 fanout-score-rank=24 prefix-density=0.61 prefix-fanout=4.9 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=342.42 fanout-score-rank=1 prefix-density=0.52 prefix-fanout=8.1 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTGCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAATAGATACAGGGTCTCAAACCTTGCTATAGAGTTTTTGCTT Started job on | Dec 07 02:24:48 Started mapping on | Dec 07 02:24:48 Finished on | Dec 07 02:24:54 Mapping speed, Million of reads per hour | 1433.36 Number of input reads | 2388934 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 1737356 Uniquely mapped reads % | 72.73% Average mapped length | 90.96 Number of splices: Total | 80943 Number of splices: Annotated (sjdb) | 66875 Number of splices: GT/AG | 76404 Number of splices: GC/AG | 1895 Number of splices: AT/AC | 30 Number of splices: Non-canonical | 2614 Mismatch rate per base, % | 0.49% Deletion rate per base | 0.04% Deletion average length | 1.77 Insertion rate per base | 0.02% Insertion average length | 1.83 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 554405 % of reads mapped to multiple loci | 23.21% Number of reads mapped to too many loci | 22643 % of reads mapped to too many loci | 0.95% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.00% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 97173 97173 97173 N_multimapping 554405 554405 554405 N_noFeature 90945 106978 1657602 N_ambiguous 69503 5791 225 UnstrandedReadsAssigned:1576908 PositiveStrandReadsAssigned:1624587 NegativeStrandReadsAssigned:79529 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133385 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133385-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,388,934 reads, 2,009,335 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,000 rounds 52973 ERR6133385.ke.tsv 35125 ERR6133385.se.tsv 88098 total ==> ERR6133385.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 60 29.9938 PNS24243 293 194 0 0 KQK14069 1603 1504 51 23.2572 KQK14071 474 375 0 0 ==> ERR6133385.se.tsv <== BRADI_1g14170v3 51 BRADI_1g53295v3 41 BRADI_1g59795v3 21 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 30 BRADI_1g74790v3 17 BRADI_1g09890v3 0 BRADI_1g77505v3 56 BRADI_1g48960v3 0 ERR6133385 completed mapping pipeline successfully