Starting /dee2/code/volunteer_pipeline.sh ERR6133387 current disk space = 1547620118528 free memory = 1600195876 ERR6133387 SRAfilesize 0db53d586667737dcc95d5830fab807c ERR6133387.sra ERR6133387.sra file validated ERR6133387 is single end ERR6133387 is conventional basespace ERR6133387 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133387_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.88225 37.0 37.0 37.0 37.0 37.0 2 36.789 37.0 37.0 37.0 37.0 37.0 3 36.6395 37.0 37.0 37.0 37.0 37.0 4 36.1635 37.0 37.0 37.0 33.0 37.0 5 36.16725 37.0 37.0 37.0 33.0 37.0 6 36.3325 37.0 37.0 37.0 37.0 37.0 7 38.42225 40.0 37.0 40.0 37.0 40.0 8 38.319 40.0 37.0 40.0 37.0 40.0 9 38.39525 40.0 37.0 40.0 37.0 40.0 10-11 38.324625 40.0 37.0 40.0 37.0 40.0 12-13 38.4105 40.0 37.0 40.0 37.0 40.0 14-15 38.362875 40.0 37.0 40.0 37.0 40.0 16-17 38.260125 40.0 37.0 40.0 37.0 40.0 18-19 38.1505 40.0 37.0 40.0 37.0 40.0 20-21 38.01275 40.0 37.0 40.0 35.0 40.0 22-23 38.000375 40.0 37.0 40.0 33.0 40.0 24-25 37.8575 40.0 37.0 40.0 33.0 40.0 26-27 37.78475 40.0 37.0 40.0 33.0 40.0 28-29 37.599374999999995 40.0 37.0 40.0 33.0 40.0 30-31 37.53725 40.0 37.0 40.0 33.0 40.0 32-33 37.30675 37.0 37.0 40.0 33.0 40.0 34-35 37.093875 37.0 37.0 40.0 33.0 40.0 36-37 37.15175 37.0 37.0 40.0 33.0 40.0 38-39 37.213625 37.0 37.0 40.0 33.0 40.0 40-41 37.410125 37.0 37.0 40.0 33.0 40.0 42-43 37.368 37.0 37.0 40.0 33.0 40.0 44-45 37.27475 37.0 37.0 40.0 33.0 40.0 46-47 36.955749999999995 37.0 37.0 40.0 33.0 40.0 48-49 36.841875 37.0 37.0 40.0 33.0 40.0 50-51 36.737625 37.0 37.0 40.0 33.0 40.0 52-53 36.617375 37.0 37.0 40.0 33.0 40.0 54-55 36.390249999999995 37.0 37.0 37.0 33.0 40.0 56-57 36.2245 37.0 37.0 37.0 33.0 40.0 58-59 36.133625 37.0 37.0 37.0 33.0 40.0 60-61 35.877875 37.0 37.0 37.0 33.0 40.0 62-63 35.617875 37.0 35.0 37.0 33.0 38.5 64-65 35.3055 37.0 33.0 37.0 33.0 37.0 66-67 35.208 37.0 33.0 37.0 33.0 37.0 68-69 34.368 35.0 33.0 37.0 30.0 37.0 70-71 34.51362688064192 37.0 33.0 37.0 33.0 37.0 72-73 34.8827819027977 37.0 33.0 37.0 33.0 37.0 74-75 34.95125888348906 37.0 33.0 37.0 33.0 37.0 76-77 34.88704582907438 37.0 33.0 37.0 33.0 37.0 78-79 34.90074737457121 37.0 33.0 37.0 33.0 37.0 80-81 34.828515347985544 37.0 33.0 37.0 33.0 37.0 82-83 34.480432142316374 37.0 33.0 37.0 33.0 37.0 84-85 34.470935968137255 37.0 33.0 37.0 33.0 37.0 86-87 34.56705882352941 37.0 33.0 37.0 33.0 37.0 88-89 34.60156862745098 37.0 33.0 37.0 33.0 37.0 90-91 34.06692810457516 37.0 33.0 37.0 27.0 37.0 92-93 33.79019607843137 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 5.0 21 7.0 22 5.0 23 9.0 24 9.0 25 17.0 26 24.0 27 14.0 28 30.0 29 36.0 30 53.0 31 73.0 32 105.0 33 119.0 34 189.0 35 328.0 36 745.0 37 1084.0 38 1094.0 39 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 74.725 5.25 5.775 14.249999999999998 2 51.7 26.525 13.725000000000001 8.05 3 31.924999999999997 36.85 17.375 13.850000000000001 4 34.0 25.224999999999998 20.5 20.275000000000002 5 20.95 32.375 27.025 19.650000000000002 6 18.925 37.65 26.05 17.375 7 31.7 29.375 23.025000000000002 15.9 8 29.049999999999997 28.125 24.275 18.55 9 24.55 27.474999999999998 28.4 19.575 10-11 24.075 28.487499999999997 28.075 19.3625 12-13 26.875 24.45 28.8625 19.8125 14-15 23.375 24.275 31.525 20.825 16-17 25.825 29.4875 26.974999999999998 17.712500000000002 18-19 23.575 26.150000000000002 27.2625 23.0125 20-21 25.2875 26.35 27.800000000000004 20.5625 22-23 26.174999999999997 25.35 26.7125 21.762500000000003 24-25 25.575 23.974999999999998 28.812500000000004 21.637500000000003 26-27 24.2375 26.487500000000004 29.725 19.55 28-29 25.4625 25.412499999999998 27.900000000000002 21.224999999999998 30-31 26.3125 25.7125 27.3 20.674999999999997 32-33 23.3 26.674999999999997 29.125 20.9 34-35 26.275 24.275 28.125 21.325 36-37 25.1 24.625 28.7 21.575 38-39 23.75 25.7625 31.075000000000003 19.412499999999998 40-41 25.837500000000002 25.4375 28.012500000000003 20.7125 42-43 24.1375 27.8875 27.9125 20.0625 44-45 24.0125 25.7625 29.1875 21.0375 46-47 24.675 25.0125 28.037499999999998 22.275 48-49 24.7875 25.6125 30.2125 19.3875 50-51 25.224999999999998 25.25 28.9375 20.5875 52-53 24.075 27.037499999999998 27.287499999999998 21.6 54-55 23.9125 28.025 27.5125 20.549999999999997 56-57 24.625 25.525 29.5875 20.2625 58-59 24.0125 24.375 29.5875 22.025 60-61 24.0625 24.6625 29.95 21.325 62-63 22.4375 27.6125 30.2125 19.7375 64-65 23.275000000000002 26.700000000000003 28.487499999999997 21.5375 66-67 24.8125 27.3 28.512500000000003 19.375 68-69 24.1375 26.487500000000004 28.575 20.8 70-71 25.776164246369554 24.44917376064096 28.605408112168252 21.16925388082123 72-73 25.490689481630596 25.26421741318571 28.799698037242074 20.44539506794162 74-75 22.524345516630834 26.710509674971544 29.809029973441252 20.95611483495637 76-77 22.462477741032817 26.812515899262273 30.488425337064363 20.23658102264055 78-79 24.338150658652 25.335720680393912 30.681672848190306 19.64445581276378 80-81 23.753213367609256 25.89974293059126 30.77120822622108 19.57583547557841 82-83 24.067357512953368 24.689119170984455 30.20725388601036 21.036269430051814 84-85 24.05740378343118 24.35746901500326 31.167645140247878 20.417482061317678 86-87 22.732026143790847 25.816993464052292 31.4640522875817 19.986928104575163 88-89 22.37908496732026 27.88235294117647 30.627450980392158 19.11111111111111 90-91 24.261437908496735 26.849673202614376 29.712418300653592 19.176470588235293 92-93 22.83660130718954 29.176470588235293 28.954248366013076 19.03267973856209 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 2.5 18 3.0 19 2.0 20 2.0 21 2.0 22 2.5 23 2.0 24 4.0 25 6.5 26 5.5 27 10.0 28 23.0 29 30.5 30 28.5 31 39.5 32 59.0 33 68.0 34 91.0 35 108.0 36 131.0 37 150.0 38 154.5 39 177.5 40 189.5 41 184.5 42 197.0 43 213.0 44 205.0 45 181.0 46 192.5 47 208.0 48 188.5 49 181.0 50 164.0 51 146.5 52 148.5 53 141.5 54 111.0 55 90.0 56 69.0 57 50.0 58 52.0 59 53.5 60 43.0 61 38.5 62 41.0 63 33.5 64 28.0 65 23.5 66 17.5 67 13.5 68 9.5 69 5.0 70 2.0 71 1.0 72 1.5 73 1.0 74 1.5 75 2.0 76 0.5 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 12.0 71 9.0 72 10.0 73 9.0 74 13.0 75 12.0 76 8.0 77 13.0 78 9.0 79 8.0 80 14.0 81 15.0 82 16.0 83 12.0 84 15.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3825.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.6 #Duplication Level Percentage of deduplicated Percentage of total 1 95.49086757990868 83.65 2 2.6255707762557075 4.6 3 0.5136986301369862 1.35 4 0.3995433789954338 1.4000000000000001 5 0.1997716894977169 0.8750000000000001 6 0.14269406392694062 0.75 7 0.14269406392694062 0.8750000000000001 8 0.08561643835616438 0.6 9 0.028538812785388126 0.22499999999999998 >10 0.3710045662100456 5.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 46 1.15 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 29 0.7250000000000001 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 19 0.475 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 16 0.4 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 16 0.4 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 16 0.4 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 16 0.4 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 13 0.325 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 12 0.3 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 12 0.3 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 12 0.3 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 10 0.25 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 10 0.25 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 9 0.22499999999999998 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 8 0.2 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 8 0.2 No Hit TAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTTGACGGCGGACT 8 0.2 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 7 0.17500000000000002 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 7 0.17500000000000002 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 7 0.17500000000000002 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 7 0.17500000000000002 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 7 0.17500000000000002 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 6 0.15 No Hit GAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGG 6 0.15 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 6 0.15 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 6 0.15 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 6 0.15 No Hit AGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATGAGCCT 5 0.125 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 5 0.125 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 5 0.125 No Hit GCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGA 5 0.125 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 5 0.125 No Hit TCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATCT 5 0.125 No Hit AAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260186 READS because READLEN < 1 Read 260186 spots for ERR6133387.sra Written 260186 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra Rejected 260184 READS because READLEN < 1 Read 260184 spots for ERR6133387.sra Written 260184 spots for ERR6133387.sra SRR ids: ['ERR6133387.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_pd9orlmu ERR6133387.sra spots: 5203682 blocks: [[1, 260184], [260185, 520368], [520369, 780552], [780553, 1040736], [1040737, 1300920], [1300921, 1561104], [1561105, 1821288], [1821289, 2081472], [2081473, 2341656], [2341657, 2601840], [2601841, 2862024], [2862025, 3122208], [3122209, 3382392], [3382393, 3642576], [3642577, 3902760], [3902761, 4162944], [4162945, 4423128], [4423129, 4683312], [4683313, 4943496], [4943497, 5203682]] ERR6133387 file size 1149257 ERR6133387 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133387 ERR6133387_1.fastq Input file: ERR6133387_1.fastq trimmed: ERR6133387-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:31:10 2024 >> started Sat Dec 7 02:31:14 2024 >> done (4.363s) 5203682 reads processed; of these: 254 ( 0.00%) short reads filtered out after trimming by size control 6 ( 0.00%) empty reads filtered out after trimming by size control 5203422 (100.00%) reads available; of these: 205889 ( 3.96%) trimmed reads available after processing 4997533 (96.04%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 28 0.00% 20 13 0.00% 21 15 0.00% 22 16 0.00% 23 19 0.00% 24 12 0.00% 25 18 0.00% 26 14 0.00% 27 13 0.00% 28 12 0.00% 29 16 0.00% 30 7 0.00% 31 17 0.00% 32 17 0.00% 33 8 0.00% 34 4 0.00% 35 11 0.00% 36 10 0.00% 37 8 0.00% 38 20 0.00% 39 54 0.00% 40 90 0.00% 41 37 0.00% 42 24 0.00% 43 31 0.00% 44 23 0.00% 45 45 0.00% 46 93 0.00% 47 149 0.00% 48 282 0.01% 49 1685 0.03% 50 470 0.01% 51 509 0.01% 52 515 0.01% 53 642 0.01% 54 678 0.01% 55 794 0.02% 56 889 0.02% 57 1136 0.02% 58 937 0.02% 59 1215 0.02% 60 1413 0.03% 61 1190 0.02% 62 1303 0.03% 63 1804 0.03% 64 1600 0.03% 65 1622 0.03% 66 1827 0.04% 67 2127 0.04% 68 2482 0.05% 69 1254 0.02% 70 15274 0.29% 71 15865 0.30% 72 19738 0.38% 73 17562 0.34% 74 17647 0.34% 75 18681 0.36% 76 17079 0.33% 77 16982 0.33% 78 18813 0.36% 79 19597 0.38% 80 19190 0.37% 81 19208 0.37% 82 22133 0.43% 83 25493 0.49% 84 22308 0.43% 85 8533 0.16% 86 9420 0.18% 87 10824 0.21% 88 13789 0.26% 89 13945 0.27% 90 18689 0.36% 91 21944 0.42% 92 22594 0.43% 93 4770902 91.69% 5203422 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=8.66 fanout-score-rank=17 prefix-density=0.57 prefix-fanout=5.0 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.06 sequence-density-rank=14 fanout-score=71.67 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=19.1 sequence=TTTTTTTTTTAAGAATCCTCCATTTTTGTTCTTCCACCCATGCAATAGAGAGCAAATGGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGAGTCATGGAATAATGGTGAATTCAAATGTTTAGTTCTTTAGTATAAGAAGTATAAGAA Started job on | Dec 07 02:31:30 Started mapping on | Dec 07 02:31:30 Finished on | Dec 07 02:31:38 Mapping speed, Million of reads per hour | 2341.54 Number of input reads | 5203422 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 3820822 Uniquely mapped reads % | 73.43% Average mapped length | 91.39 Number of splices: Total | 201014 Number of splices: Annotated (sjdb) | 163803 Number of splices: GT/AG | 192010 Number of splices: GC/AG | 4731 Number of splices: AT/AC | 48 Number of splices: Non-canonical | 4225 Mismatch rate per base, % | 0.57% Deletion rate per base | 0.05% Deletion average length | 1.79 Insertion rate per base | 0.03% Insertion average length | 1.90 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1183113 % of reads mapped to multiple loci | 22.74% Number of reads mapped to too many loci | 61541 % of reads mapped to too many loci | 1.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.50% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 199487 199487 199487 N_multimapping 1183113 1183113 1183113 N_noFeature 216526 250308 3647383 N_ambiguous 154665 14887 650 UnstrandedReadsAssigned:3449631 PositiveStrandReadsAssigned:3555627 NegativeStrandReadsAssigned:172789 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133387 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133387-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 5,203,422 reads, 4,329,457 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,046 rounds 52973 ERR6133387.ke.tsv 35125 ERR6133387.se.tsv 88098 total ==> ERR6133387.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 99 22.7543 PNS24243 293 194 0 0 KQK14069 1603 1504 179 37.5307 KQK14071 474 375 1 0.840911 ==> ERR6133387.se.tsv <== BRADI_1g14170v3 181 BRADI_1g53295v3 73 BRADI_1g59795v3 44 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 78 BRADI_1g74790v3 45 BRADI_1g09890v3 0 BRADI_1g77505v3 94 BRADI_1g48960v3 0 ERR6133387 completed mapping pipeline successfully