Starting /dee2/code/volunteer_pipeline.sh ERR6133390 current disk space = 1547242577920 free memory = 1602158688 ERR6133390 SRAfilesize 3acfb72a857a199e841480a615692f84 ERR6133390.sra ERR6133390.sra file validated ERR6133390 is single end ERR6133390 is conventional basespace ERR6133390 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133390_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.9015 37.0 37.0 37.0 37.0 37.0 2 36.79775 37.0 37.0 37.0 37.0 37.0 3 36.555 37.0 37.0 37.0 37.0 37.0 4 36.0995 37.0 37.0 37.0 33.0 37.0 5 36.04475 37.0 37.0 37.0 33.0 37.0 6 36.22 37.0 37.0 37.0 33.0 37.0 7 38.15775 40.0 37.0 40.0 37.0 40.0 8 38.1155 40.0 37.0 40.0 37.0 40.0 9 38.2115 40.0 37.0 40.0 37.0 40.0 10-11 38.117625000000004 40.0 37.0 40.0 37.0 40.0 12-13 38.170875 40.0 37.0 40.0 37.0 40.0 14-15 38.08975 40.0 37.0 40.0 37.0 40.0 16-17 38.040499999999994 40.0 37.0 40.0 35.0 40.0 18-19 37.985375000000005 40.0 37.0 40.0 33.0 40.0 20-21 37.873 40.0 37.0 40.0 33.0 40.0 22-23 37.861000000000004 40.0 37.0 40.0 33.0 40.0 24-25 37.65325 40.0 37.0 40.0 33.0 40.0 26-27 37.492625000000004 38.5 37.0 40.0 33.0 40.0 28-29 37.315375 37.0 37.0 40.0 33.0 40.0 30-31 37.229124999999996 37.0 37.0 40.0 33.0 40.0 32-33 37.092625 37.0 37.0 40.0 33.0 40.0 34-35 36.877375 37.0 37.0 40.0 33.0 40.0 36-37 36.87425 37.0 37.0 40.0 33.0 40.0 38-39 37.040875 37.0 37.0 40.0 33.0 40.0 40-41 37.212999999999994 37.0 37.0 40.0 33.0 40.0 42-43 37.049125000000004 37.0 37.0 40.0 33.0 40.0 44-45 37.037499999999994 37.0 37.0 40.0 33.0 40.0 46-47 36.779125 37.0 37.0 40.0 33.0 40.0 48-49 36.6505 37.0 37.0 40.0 33.0 40.0 50-51 36.524875 37.0 37.0 40.0 33.0 40.0 52-53 36.3545 37.0 37.0 37.0 33.0 40.0 54-55 36.152125 37.0 37.0 37.0 33.0 40.0 56-57 36.032875000000004 37.0 37.0 37.0 33.0 40.0 58-59 35.8845 37.0 37.0 37.0 33.0 40.0 60-61 35.836625 37.0 37.0 37.0 33.0 37.0 62-63 35.589124999999996 37.0 35.0 37.0 33.0 37.0 64-65 35.399249999999995 37.0 33.0 37.0 33.0 37.0 66-67 35.237625 37.0 33.0 37.0 33.0 37.0 68-69 34.384625 35.0 33.0 37.0 30.0 37.0 70-71 34.51118953634085 37.0 33.0 37.0 33.0 37.0 72-73 34.941549423468416 37.0 33.0 37.0 33.0 37.0 74-75 35.06642013755729 37.0 33.0 37.0 33.0 37.0 76-77 34.953088546348965 37.0 33.0 37.0 33.0 37.0 78-79 35.04421318504383 37.0 33.0 37.0 33.0 37.0 80-81 34.933097211614054 37.0 33.0 37.0 33.0 37.0 82-83 34.625333718855615 37.0 33.0 37.0 33.0 37.0 84-85 34.67732268116451 37.0 33.0 37.0 33.0 37.0 86-87 34.625525210084035 37.0 33.0 37.0 33.0 37.0 88-89 34.62001050420169 37.0 33.0 37.0 33.0 37.0 90-91 34.269695378151255 37.0 33.0 37.0 33.0 37.0 92-93 34.055015756302524 37.0 33.0 37.0 30.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 6.0 21 6.0 22 8.0 23 6.0 24 9.0 25 18.0 26 14.0 27 30.0 28 41.0 29 48.0 30 44.0 31 63.0 32 81.0 33 130.0 34 184.0 35 339.0 36 900.0 37 1069.0 38 964.0 39 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 84.925 3.9 3.1 8.075000000000001 2 63.7 21.675 9.45 5.175 3 33.475 38.425 15.975 12.125 4 35.125 26.450000000000003 19.75 18.675 5 21.675 34.125 27.0 17.2 6 20.775 36.7 26.125 16.400000000000002 7 36.65 28.95 19.425 14.975 8 30.225 30.099999999999998 23.0 16.675 9 27.200000000000003 27.200000000000003 26.325 19.275000000000002 10-11 24.775 28.812500000000004 27.737499999999997 18.675 12-13 28.3875 24.8625 28.625 18.125 14-15 22.55 26.25 31.45 19.75 16-17 24.875 31.0375 26.437500000000004 17.65 18-19 24.2 25.837500000000002 27.35 22.6125 20-21 25.837500000000002 25.2625 27.762500000000003 21.1375 22-23 26.4625 24.4125 28.3125 20.8125 24-25 24.675 24.025 29.3375 21.9625 26-27 25.0125 24.7 30.175 20.1125 28-29 25.025 26.424999999999997 27.8375 20.7125 30-31 26.85 25.687500000000004 26.950000000000003 20.5125 32-33 24.4 27.0875 28.65 19.8625 34-35 25.137500000000003 24.525 29.7125 20.625 36-37 24.725 24.099999999999998 28.749999999999996 22.425 38-39 25.575 24.1875 30.725 19.5125 40-41 26.200000000000003 25.337500000000002 28.3125 20.150000000000002 42-43 24.1375 27.1 28.425 20.3375 44-45 23.724999999999998 24.8 29.825000000000003 21.65 46-47 25.0 24.4375 28.962500000000002 21.6 48-49 24.525 24.95 30.0375 20.4875 50-51 24.9125 25.924999999999997 28.825 20.3375 52-53 24.5 26.137500000000003 28.7 20.6625 54-55 23.962500000000002 27.712500000000002 28.212500000000002 20.1125 56-57 24.1875 26.687499999999996 29.3375 19.787499999999998 58-59 22.3375 26.0625 30.837500000000002 20.7625 60-61 24.212500000000002 24.85 30.325000000000003 20.6125 62-63 22.025 28.1125 31.0 18.862499999999997 64-65 24.1125 26.75 28.8875 20.25 66-67 24.962500000000002 26.200000000000003 28.749999999999996 20.0875 68-69 23.4125 26.3 29.5375 20.75 70-71 24.518147684605758 24.893617021276597 29.662077596996244 20.926157697121404 72-73 25.330479667631877 25.091275336774522 29.686516429560623 19.891728566032985 74-75 23.5085497150095 26.29512349588347 29.778340721975933 20.417986067131096 76-77 23.365200764818354 25.55768005098789 30.681963033779475 20.395156150414277 78-79 24.240481989488526 24.855787719523136 30.59864119984617 20.30508909114216 80-81 22.691463257135478 27.250419733953247 30.207929742993674 19.850187265917604 82-83 24.154086413326393 25.546590317542943 30.12753774076002 20.171785528370638 84-85 23.79454926624738 24.43658280922432 31.22379454926625 20.545073375262053 86-87 23.266806722689076 25.603991596638654 30.81670168067227 20.3125 88-89 22.137605042016805 27.862394957983195 30.88235294117647 19.11764705882353 90-91 25.275735294117645 26.339285714285715 29.16228991596639 19.222689075630253 92-93 23.76575630252101 26.93014705882353 30.252100840336134 19.051995798319325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.5 18 4.0 19 1.0 20 2.0 21 2.5 22 2.5 23 3.0 24 3.5 25 4.5 26 8.0 27 12.0 28 17.5 29 23.0 30 23.0 31 31.5 32 50.5 33 74.5 34 97.0 35 110.5 36 133.0 37 154.0 38 167.5 39 173.5 40 189.0 41 190.5 42 189.5 43 203.0 44 197.5 45 185.5 46 205.0 47 221.0 48 189.5 49 159.5 50 163.0 51 153.5 52 135.0 53 137.5 54 121.5 55 86.5 56 60.5 57 57.0 58 57.5 59 60.0 60 48.5 61 35.5 62 28.5 63 26.0 64 28.0 65 22.0 66 17.0 67 15.0 68 12.5 69 9.5 70 6.5 71 4.0 72 4.0 73 3.0 74 1.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 10.0 71 12.0 72 13.0 73 14.0 74 7.0 75 16.0 76 11.0 77 9.0 78 15.0 79 14.0 80 15.0 81 16.0 82 12.0 83 12.0 84 16.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3808.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.45 #Duplication Level Percentage of deduplicated Percentage of total 1 94.33962264150944 82.5 2 3.516295025728988 6.15 3 1.0291595197255576 2.7 4 0.37164093767867357 1.3 5 0.11435105774728416 0.5 6 0.02858776443682104 0.15 7 0.11435105774728416 0.7000000000000001 8 0.11435105774728416 0.8 9 0.08576329331046312 0.675 >10 0.2858776443682104 4.5249999999999995 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 35 0.8750000000000001 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 27 0.675 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 26 0.65 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 21 0.525 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 15 0.375 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 14 0.35000000000000003 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 12 0.3 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 11 0.27499999999999997 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 10 0.25 No Hit AGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATGAGCCT 10 0.25 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 9 0.22499999999999998 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 9 0.22499999999999998 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 9 0.22499999999999998 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 8 0.2 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 8 0.2 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 8 0.2 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 8 0.2 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 7 0.17500000000000002 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 7 0.17500000000000002 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 7 0.17500000000000002 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 7 0.17500000000000002 No Hit TCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATCT 6 0.15 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 5 0.125 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0125 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245668 READS because READLEN < 1 Read 245668 spots for ERR6133390.sra Written 245668 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra Rejected 245660 READS because READLEN < 1 Read 245660 spots for ERR6133390.sra Written 245660 spots for ERR6133390.sra SRR ids: ['ERR6133390.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_awhlzq00 ERR6133390.sra spots: 4913208 blocks: [[1, 245660], [245661, 491320], [491321, 736980], [736981, 982640], [982641, 1228300], [1228301, 1473960], [1473961, 1719620], [1719621, 1965280], [1965281, 2210940], [2210941, 2456600], [2456601, 2702260], [2702261, 2947920], [2947921, 3193580], [3193581, 3439240], [3439241, 3684900], [3684901, 3930560], [3930561, 4176220], [4176221, 4421880], [4421881, 4667540], [4667541, 4913208]] ERR6133390 file size 1084474 ERR6133390 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133390 ERR6133390_1.fastq Input file: ERR6133390_1.fastq trimmed: ERR6133390-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:50:16 2024 >> started Sat Dec 7 02:50:19 2024 >> done (2.610s) 4913208 reads processed; of these: 260 ( 0.01%) short reads filtered out after trimming by size control 11 ( 0.00%) empty reads filtered out after trimming by size control 4912937 (99.99%) reads available; of these: 189706 ( 3.86%) trimmed reads available after processing 4723231 (96.14%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 55 0.00% 20 20 0.00% 21 11 0.00% 22 17 0.00% 23 9 0.00% 24 17 0.00% 25 9 0.00% 26 18 0.00% 27 24 0.00% 28 11 0.00% 29 98 0.00% 30 8 0.00% 31 21 0.00% 32 37 0.00% 33 3 0.00% 34 13 0.00% 35 13 0.00% 36 8 0.00% 37 18 0.00% 38 27 0.00% 39 73 0.00% 40 160 0.00% 41 31 0.00% 42 18 0.00% 43 34 0.00% 44 34 0.00% 45 50 0.00% 46 91 0.00% 47 117 0.00% 48 260 0.01% 49 1551 0.03% 50 414 0.01% 51 492 0.01% 52 493 0.01% 53 578 0.01% 54 572 0.01% 55 699 0.01% 56 807 0.02% 57 820 0.02% 58 956 0.02% 59 970 0.02% 60 1401 0.03% 61 1111 0.02% 62 1195 0.02% 63 1521 0.03% 64 1351 0.03% 65 1491 0.03% 66 1762 0.04% 67 1876 0.04% 68 2245 0.05% 69 1107 0.02% 70 15884 0.32% 71 16675 0.34% 72 19418 0.40% 73 17395 0.35% 74 17639 0.36% 75 18828 0.38% 76 17064 0.35% 77 16999 0.35% 78 18738 0.38% 79 19813 0.40% 80 18627 0.38% 81 18958 0.39% 82 21362 0.43% 83 24669 0.50% 84 20758 0.42% 85 7841 0.16% 86 8760 0.18% 87 10069 0.20% 88 13046 0.27% 89 13079 0.27% 90 17530 0.36% 91 19243 0.39% 92 21330 0.43% 93 4494479 91.48% 4912937 reads passed initial QC criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=5.57 fanout-score-rank=22 prefix-density=0.62 prefix-fanout=3.9 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=90.42 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=7.3 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTATAGGGAAAACATAATTTATGAGACAATCATACTGTGCAACTCTTGGCTCCATCGATTAATTAATACTCCTTGTTATTGCTTGCATGGTGG Started job on | Dec 07 02:50:33 Started mapping on | Dec 07 02:50:34 Finished on | Dec 07 02:50:43 Mapping speed, Million of reads per hour | 1965.17 Number of input reads | 4912937 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 3921566 Uniquely mapped reads % | 79.82% Average mapped length | 91.40 Number of splices: Total | 184067 Number of splices: Annotated (sjdb) | 152507 Number of splices: GT/AG | 174318 Number of splices: GC/AG | 5109 Number of splices: AT/AC | 34 Number of splices: Non-canonical | 4606 Mismatch rate per base, % | 0.52% Deletion rate per base | 0.04% Deletion average length | 1.82 Insertion rate per base | 0.03% Insertion average length | 1.76 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 815821 % of reads mapped to multiple loci | 16.61% Number of reads mapped to too many loci | 32139 % of reads mapped to too many loci | 0.65% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.83% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 175550 175550 175550 N_multimapping 815821 815821 815821 N_noFeature 183660 220656 3730014 N_ambiguous 167569 13110 379 UnstrandedReadsAssigned:3570337 PositiveStrandReadsAssigned:3687800 NegativeStrandReadsAssigned:191173 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133390 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133390-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 4,912,937 reads, 4,159,849 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,053 rounds 52973 ERR6133390.ke.tsv 35125 ERR6133390.se.tsv 88098 total ==> ERR6133390.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 150 36.2185 PNS24243 293 194 0 0 KQK14069 1603 1504 221 48.6785 KQK14071 474 375 0 0 ==> ERR6133390.se.tsv <== BRADI_1g14170v3 220 BRADI_1g53295v3 128 BRADI_1g59795v3 30 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 40 BRADI_1g74790v3 56 BRADI_1g09890v3 0 BRADI_1g77505v3 147 BRADI_1g48960v3 0 ERR6133390 completed mapping pipeline successfully