Starting /dee2/code/volunteer_pipeline.sh ERR6133391 current disk space = 1547242577920 free memory = 1599395212 ERR6133391 SRAfilesize 284be7974384fd56e834655f8807f97e ERR6133391.sra ERR6133391.sra file validated ERR6133391 is single end ERR6133391 is conventional basespace ERR6133391 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133391_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.90125 37.0 37.0 37.0 37.0 37.0 2 36.7965 37.0 37.0 37.0 37.0 37.0 3 36.5655 37.0 37.0 37.0 37.0 37.0 4 36.10225 37.0 37.0 37.0 33.0 37.0 5 35.9755 37.0 37.0 37.0 33.0 37.0 6 36.24125 37.0 37.0 37.0 33.0 37.0 7 38.20475 40.0 37.0 40.0 37.0 40.0 8 38.11975 40.0 37.0 40.0 33.0 40.0 9 38.19725 40.0 37.0 40.0 37.0 40.0 10-11 38.18575 40.0 37.0 40.0 37.0 40.0 12-13 38.221125 40.0 37.0 40.0 37.0 40.0 14-15 38.081 40.0 37.0 40.0 37.0 40.0 16-17 38.02575 40.0 37.0 40.0 35.0 40.0 18-19 37.98975 40.0 37.0 40.0 33.0 40.0 20-21 37.86 40.0 37.0 40.0 33.0 40.0 22-23 37.718875 40.0 37.0 40.0 33.0 40.0 24-25 37.56125 40.0 37.0 40.0 33.0 40.0 26-27 37.5005 38.5 37.0 40.0 33.0 40.0 28-29 37.29275 37.0 37.0 40.0 33.0 40.0 30-31 37.21925 37.0 37.0 40.0 33.0 40.0 32-33 36.992375 37.0 37.0 40.0 33.0 40.0 34-35 36.913375 37.0 37.0 40.0 33.0 40.0 36-37 36.807 37.0 37.0 40.0 33.0 40.0 38-39 36.89575 37.0 37.0 40.0 33.0 40.0 40-41 37.191874999999996 37.0 37.0 40.0 33.0 40.0 42-43 37.13175 37.0 37.0 40.0 33.0 40.0 44-45 36.953374999999994 37.0 37.0 40.0 33.0 40.0 46-47 36.765125 37.0 37.0 40.0 33.0 40.0 48-49 36.668875 37.0 37.0 40.0 33.0 40.0 50-51 36.52575 37.0 37.0 40.0 33.0 40.0 52-53 36.36075 37.0 37.0 37.0 33.0 40.0 54-55 36.053125 37.0 37.0 37.0 33.0 40.0 56-57 35.888000000000005 37.0 37.0 37.0 33.0 40.0 58-59 35.781625000000005 37.0 37.0 37.0 33.0 40.0 60-61 35.642125 37.0 35.0 37.0 33.0 37.0 62-63 35.353375 37.0 33.0 37.0 33.0 37.0 64-65 35.084125 37.0 33.0 37.0 33.0 37.0 66-67 35.00125 37.0 33.0 37.0 33.0 37.0 68-69 34.15275 35.0 33.0 37.0 30.0 37.0 70-71 34.288412593984965 37.0 33.0 37.0 33.0 37.0 72-73 34.49474011399835 37.0 33.0 37.0 30.0 37.0 74-75 34.6403258319979 37.0 33.0 37.0 33.0 37.0 76-77 34.61093219996769 37.0 33.0 37.0 33.0 37.0 78-79 34.79624173921059 37.0 33.0 37.0 33.0 37.0 80-81 34.51382983841464 37.0 33.0 37.0 30.0 37.0 82-83 34.04914244040627 37.0 33.0 37.0 27.0 37.0 84-85 34.20760142309372 37.0 33.0 37.0 30.0 37.0 86-87 34.28509916492693 37.0 33.0 37.0 30.0 37.0 88-89 34.26748434237996 37.0 33.0 37.0 33.0 37.0 90-91 33.64418058455115 37.0 33.0 37.0 27.0 37.0 92-93 33.66453549060543 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 9.0 22 9.0 23 8.0 24 17.0 25 17.0 26 27.0 27 33.0 28 37.0 29 47.0 30 62.0 31 71.0 32 102.0 33 139.0 34 184.0 35 361.0 36 849.0 37 1037.0 38 941.0 39 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 80.625 5.525 4.9 8.95 2 58.025000000000006 24.349999999999998 11.275 6.35 3 33.25 36.575 17.025000000000002 13.15 4 33.575 26.974999999999998 19.225 20.225 5 23.425 31.724999999999998 25.75 19.1 6 19.675 37.25 26.400000000000002 16.675 7 34.425 30.225 19.825 15.525 8 30.55 28.65 23.849999999999998 16.950000000000003 9 26.174999999999997 24.95 28.749999999999996 20.125 10-11 25.0 27.725 27.275 20.0 12-13 28.0625 24.9375 28.812500000000004 18.1875 14-15 21.8875 26.525 31.087500000000002 20.5 16-17 24.224999999999998 30.5375 26.974999999999998 18.2625 18-19 23.6375 26.387500000000003 26.8625 23.1125 20-21 25.887500000000003 26.05 27.8875 20.175 22-23 27.55 23.125 28.475 20.849999999999998 24-25 26.650000000000002 23.65 27.987499999999997 21.712500000000002 26-27 25.637500000000003 25.087500000000002 29.6375 19.6375 28-29 25.7125 26.724999999999998 27.4125 20.150000000000002 30-31 26.2625 25.087500000000002 27.2625 21.3875 32-33 24.175 26.687499999999996 28.712500000000002 20.424999999999997 34-35 25.724999999999998 24.525 28.5875 21.1625 36-37 25.0 24.4875 29.1125 21.4 38-39 25.474999999999998 25.674999999999997 28.962500000000002 19.8875 40-41 26.4125 24.675 28.1 20.8125 42-43 24.1125 27.8625 28.249999999999996 19.775000000000002 44-45 23.6625 25.224999999999998 29.4375 21.675 46-47 24.4875 24.3875 27.987499999999997 23.1375 48-49 24.2 25.8 29.512500000000003 20.4875 50-51 25.374999999999996 25.662499999999998 28.0875 20.875 52-53 26.174999999999997 25.912499999999998 26.9625 20.95 54-55 23.8125 27.8875 27.4125 20.8875 56-57 24.0 26.387500000000003 29.599999999999998 20.0125 58-59 23.400000000000002 24.875 29.212500000000002 22.5125 60-61 24.474999999999998 24.6875 29.612500000000004 21.224999999999998 62-63 22.787499999999998 27.575 30.3875 19.25 64-65 23.974999999999998 26.2125 29.862499999999997 19.950000000000003 66-67 25.7125 26.875 28.425 18.987499999999997 68-69 23.674999999999997 26.087500000000002 29.525000000000002 20.7125 70-71 24.60575719649562 24.96871088861076 28.86107634543179 21.564455569461828 72-73 26.040487866214008 24.971708789136173 28.32893247831007 20.658870866339747 74-75 22.943558592761327 26.208554796254113 30.017717033662368 20.830169577322195 76-77 23.009412363266343 27.194098193843807 28.669549732892396 21.126939709997455 78-79 24.55893633341856 25.70953720276144 29.21247762720532 20.519048836614676 80-81 23.740359897172237 27.76349614395887 29.13881748071979 19.3573264781491 82-83 25.36888428682371 24.566399171628266 30.49443437742687 19.57028216412115 84-85 24.18198409594577 24.53395906661452 30.36110024768609 20.922956589753618 86-87 21.88152400835073 26.52661795407098 32.2286012526096 19.363256784968684 88-89 21.933716075156575 29.318893528183715 30.532359081419624 18.215031315240083 90-91 24.112734864300624 27.38778705636743 28.97964509394572 19.51983298538622 92-93 23.434237995824635 27.805323590814197 28.927453027139876 19.832985386221296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 7.5 18 7.5 19 2.5 20 4.0 21 3.5 22 2.0 23 3.0 24 3.5 25 1.5 26 4.0 27 11.5 28 12.5 29 10.5 30 21.0 31 45.0 32 58.0 33 73.0 34 93.0 35 90.0 36 108.5 37 140.5 38 149.0 39 153.0 40 179.0 41 200.5 42 200.5 43 200.0 44 192.0 45 181.0 46 210.0 47 214.5 48 174.0 49 170.5 50 176.0 51 171.0 52 149.0 53 130.0 54 119.5 55 96.0 56 71.5 57 60.0 58 61.5 59 54.5 60 42.0 61 34.5 62 31.0 63 32.0 64 31.0 65 31.0 66 24.5 67 11.5 68 9.0 69 9.5 70 7.5 71 7.0 72 3.5 73 1.5 74 2.5 75 1.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 10.0 71 7.0 72 13.0 73 14.0 74 10.0 75 10.0 76 10.0 77 11.0 78 8.0 79 11.0 80 12.0 81 17.0 82 8.0 83 20.0 84 7.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3832.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.975 #Duplication Level Percentage of deduplicated Percentage of total 1 94.10538850848467 79.025 2 3.632033343256922 6.1 3 0.6549568323905924 1.6500000000000001 4 0.4763322417386127 1.6 5 0.3274784161952962 1.375 6 0.029770765108663295 0.15 7 0.17862459065197975 1.05 8 0.029770765108663295 0.2 9 0.029770765108663295 0.22499999999999998 >10 0.5358737719559392 8.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 36 0.8999999999999999 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 36 0.8999999999999999 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 29 0.7250000000000001 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 27 0.675 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 25 0.625 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 22 0.5499999999999999 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 21 0.525 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 20 0.5 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 17 0.42500000000000004 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 16 0.4 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 14 0.35000000000000003 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 14 0.35000000000000003 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 14 0.35000000000000003 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 12 0.3 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 12 0.3 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 10 0.25 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 10 0.25 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 10 0.25 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 9 0.22499999999999998 No Hit TAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTTGACGGCGGACT 8 0.2 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 7 0.17500000000000002 No Hit GAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGA 7 0.17500000000000002 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 7 0.17500000000000002 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 7 0.17500000000000002 No Hit AACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGC 7 0.17500000000000002 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 7 0.17500000000000002 No Hit GTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCA 6 0.15 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 5 0.125 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 5 0.125 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 5 0.125 No Hit GGGCCTGTTATCTCTATCAATATGATTCTAATTCGTCAGATATTATTTAT 5 0.125 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 5 0.125 No Hit GGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTC 5 0.125 No Hit GATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGGG 5 0.125 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTA 5 0.125 No Hit GAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACTAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0125 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440606 READS because READLEN < 1 Read 440606 spots for ERR6133391.sra Written 440606 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra Rejected 440603 READS because READLEN < 1 Read 440603 spots for ERR6133391.sra Written 440603 spots for ERR6133391.sra SRR ids: ['ERR6133391.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0di1o6p0 ERR6133391.sra spots: 8812063 blocks: [[1, 440603], [440604, 881206], [881207, 1321809], [1321810, 1762412], [1762413, 2203015], [2203016, 2643618], [2643619, 3084221], [3084222, 3524824], [3524825, 3965427], [3965428, 4406030], [4406031, 4846633], [4846634, 5287236], [5287237, 5727839], [5727840, 6168442], [6168443, 6609045], [6609046, 7049648], [7049649, 7490251], [7490252, 7930854], [7930855, 8371457], [8371458, 8812063]] ERR6133391 file size 1945661 ERR6133391 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133391 ERR6133391_1.fastq Input file: ERR6133391_1.fastq trimmed: ERR6133391-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 02:50:24 2024 >> started Sat Dec 7 02:50:29 2024 >> done (4.450s) 8812063 reads processed; of these: 680 ( 0.01%) short reads filtered out after trimming by size control 17 ( 0.00%) empty reads filtered out after trimming by size control 8811366 (99.99%) reads available; of these: 361546 ( 4.10%) trimmed reads available after processing 8449820 (95.90%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 65 0.00% 19 75 0.00% 20 46 0.00% 21 19 0.00% 22 42 0.00% 23 19 0.00% 24 34 0.00% 25 25 0.00% 26 26 0.00% 27 30 0.00% 28 33 0.00% 29 38 0.00% 30 27 0.00% 31 29 0.00% 32 67 0.00% 33 25 0.00% 34 20 0.00% 35 24 0.00% 36 19 0.00% 37 40 0.00% 38 97 0.00% 39 136 0.00% 40 255 0.00% 41 53 0.00% 42 51 0.00% 43 55 0.00% 44 64 0.00% 45 80 0.00% 46 155 0.00% 47 259 0.00% 48 417 0.00% 49 2714 0.03% 50 804 0.01% 51 939 0.01% 52 878 0.01% 53 1045 0.01% 54 1169 0.01% 55 1412 0.02% 56 1520 0.02% 57 1800 0.02% 58 1772 0.02% 59 2034 0.02% 60 2548 0.03% 61 2075 0.02% 62 2304 0.03% 63 3117 0.04% 64 2811 0.03% 65 2890 0.03% 66 3324 0.04% 67 3623 0.04% 68 4508 0.05% 69 2178 0.02% 70 29385 0.33% 71 30629 0.35% 72 38335 0.44% 73 32505 0.37% 74 33513 0.38% 75 36405 0.41% 76 30996 0.35% 77 32344 0.37% 78 38531 0.44% 79 40070 0.45% 80 36281 0.41% 81 36157 0.41% 82 42990 0.49% 83 55484 0.63% 84 41395 0.47% 85 14610 0.17% 86 16545 0.19% 87 19745 0.22% 88 24231 0.27% 89 24158 0.27% 90 32454 0.37% 91 37125 0.42% 92 40620 0.46% 93 7999068 90.78% 8811366 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=7.17 fanout-score-rank=23 prefix-density=0.60 prefix-fanout=2.8 sequence=GCCGCCGCCGCCAAGGAAGGCATGTTCGTCAAGAACTACAGCTACTGATCCTAATCGCATCAAGCTTCAACGCCTGTGAGTGAAAACCAGTGATGAGAGTGCTGCTGCTAGCTAGCGCCGGCATTGATGAGCTTGAGAGGGCACTGTAGCCAGTGTGTCAGTCGTTGTTAAATTACAGGTTGAGATCATCAGCGTACTCCGATGGGAGGTGGACATCAGAAAGTATACTGTGTTTTACCACCCTAATTAAGTAAACAACTTTTGGAATGGTGATCATCTTACTGTTTTAAATAAATCTGTTTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=298.18 fanout-score-rank=1 prefix-density=0.53 prefix-fanout=7.1 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTGCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAATAGATACAGGGTCTCAAACCTTGCTATAGAGTTTTTGCTT Started job on | Dec 07 02:50:46 Started mapping on | Dec 07 02:50:47 Finished on | Dec 07 02:50:59 Mapping speed, Million of reads per hour | 2643.41 Number of input reads | 8811366 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 6509419 Uniquely mapped reads % | 73.88% Average mapped length | 91.29 Number of splices: Total | 314146 Number of splices: Annotated (sjdb) | 254232 Number of splices: GT/AG | 295742 Number of splices: GC/AG | 6971 Number of splices: AT/AC | 86 Number of splices: Non-canonical | 11347 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.03% Deletion average length | 1.84 Insertion rate per base | 0.02% Insertion average length | 1.96 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1978423 % of reads mapped to multiple loci | 22.45% Number of reads mapped to too many loci | 88585 % of reads mapped to too many loci | 1.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.54% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 323524 323524 323524 N_multimapping 1978423 1978423 1978423 N_noFeature 335655 396919 6209556 N_ambiguous 259717 20983 848 UnstrandedReadsAssigned:5914047 PositiveStrandReadsAssigned:6091517 NegativeStrandReadsAssigned:299015 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133391 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133391-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 8,811,366 reads, 7,361,923 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,116 rounds 52973 ERR6133391.ke.tsv 35125 ERR6133391.se.tsv 88098 total ==> ERR6133391.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 185 24.9114 PNS24243 293 194 0 0 KQK14069 1603 1504 130 15.9689 KQK14071 474 375 0 0 ==> ERR6133391.se.tsv <== BRADI_1g14170v3 130 BRADI_1g53295v3 108 BRADI_1g59795v3 62 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 89 BRADI_1g74790v3 57 BRADI_1g09890v3 0 BRADI_1g77505v3 199 BRADI_1g48960v3 0 ERR6133391 completed mapping pipeline successfully