Starting /dee2/code/volunteer_pipeline.sh ERR6133427 current disk space = 1546780848128 free memory = 1601947292 ERR6133427 SRAfilesize 315a57079d8ba9b591cf89adcab925b3 ERR6133427.sra ERR6133427.sra file validated ERR6133427 is single end ERR6133427 is conventional basespace ERR6133427 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133427_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.21925 37.0 33.0 37.0 33.0 37.0 2 36.26475 37.0 37.0 37.0 33.0 37.0 3 35.7065 37.0 37.0 37.0 33.0 37.0 4 35.47 37.0 37.0 37.0 33.0 37.0 5 35.241 37.0 37.0 37.0 33.0 37.0 6 35.53475 37.0 37.0 37.0 33.0 37.0 7 37.2405 37.0 37.0 40.0 33.0 40.0 8 37.232 37.0 37.0 40.0 33.0 40.0 9 37.3515 37.0 37.0 40.0 33.0 40.0 10-11 37.334999999999994 37.0 37.0 40.0 33.0 40.0 12-13 37.346 37.0 37.0 40.0 33.0 40.0 14-15 37.208749999999995 37.0 37.0 40.0 33.0 40.0 16-17 37.0465 37.0 37.0 40.0 33.0 40.0 18-19 37.21525 37.0 37.0 40.0 33.0 40.0 20-21 37.403125 37.0 37.0 40.0 33.0 40.0 22-23 37.2975 37.0 37.0 40.0 33.0 40.0 24-25 37.24675 37.0 37.0 40.0 33.0 40.0 26-27 37.002125 37.0 37.0 40.0 33.0 40.0 28-29 37.14875 37.0 37.0 40.0 33.0 40.0 30-31 37.187124999999995 37.0 37.0 40.0 33.0 40.0 32-33 36.961375000000004 37.0 37.0 40.0 33.0 40.0 34-35 36.821250000000006 37.0 37.0 40.0 33.0 40.0 36-37 36.8335 37.0 37.0 40.0 33.0 40.0 38-39 36.703625 37.0 37.0 40.0 33.0 40.0 40-41 36.53875 37.0 37.0 40.0 33.0 40.0 42-43 36.389375 37.0 37.0 40.0 33.0 40.0 44-45 36.1175 37.0 37.0 38.5 33.0 40.0 46-47 36.137 37.0 37.0 37.0 33.0 40.0 48-49 35.904125 37.0 35.0 37.0 33.0 40.0 50-51 35.734 37.0 35.0 37.0 33.0 40.0 52-53 35.60575 37.0 33.0 37.0 33.0 40.0 54-55 35.43925 37.0 33.0 37.0 33.0 38.5 56-57 35.26349999999999 37.0 33.0 37.0 33.0 37.0 58-59 34.8995 37.0 33.0 37.0 33.0 37.0 60-61 34.78575 37.0 33.0 37.0 33.0 37.0 62-63 34.70675 37.0 33.0 37.0 33.0 37.0 64-65 34.33175 37.0 33.0 37.0 30.0 37.0 66-67 34.2945 37.0 33.0 37.0 27.0 37.0 68-69 33.009625 35.0 33.0 35.0 27.0 37.0 70-71 33.367122029021765 33.0 33.0 37.0 27.0 37.0 72-73 34.09198014502901 37.0 33.0 37.0 27.0 37.0 74-75 34.353908674133976 37.0 33.0 37.0 33.0 37.0 76-77 34.25980257061327 37.0 33.0 37.0 30.0 37.0 78-79 34.29905808671858 37.0 33.0 37.0 30.0 37.0 80-81 34.257258268114114 37.0 33.0 37.0 33.0 37.0 82-83 34.053079565388515 37.0 33.0 37.0 30.0 37.0 84-85 33.92073664210337 37.0 33.0 37.0 30.0 37.0 86-87 33.7606490872211 37.0 33.0 37.0 27.0 37.0 88-89 33.75709939148073 37.0 33.0 37.0 27.0 37.0 90-91 33.592038539553755 35.0 33.0 37.0 27.0 37.0 92-93 33.37068965517241 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 10.0 21 11.0 22 9.0 23 20.0 24 26.0 25 20.0 26 28.0 27 30.0 28 50.0 29 51.0 30 65.0 31 114.0 32 142.0 33 137.0 34 249.0 35 506.0 36 985.0 37 1114.0 38 430.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 88.97500000000001 2.875 1.7500000000000002 6.4 2 72.87074148296593 16.45791583166333 6.287575150300601 4.383767535070141 3 37.425000000000004 37.9 14.025000000000002 10.65 4 37.525 26.650000000000002 17.375 18.45 5 24.45 32.6 23.5 19.45 6 20.1 38.95 23.775 17.175 7 36.25 28.249999999999996 19.55 15.950000000000001 8 32.225 29.025000000000002 21.85 16.900000000000002 9 27.900000000000002 28.075 26.1 17.925 10-11 25.8625 27.1375 26.5 20.5 12-13 29.4875 24.125 26.55 19.8375 14-15 23.825 28.375 29.212500000000002 18.587500000000002 16-17 25.837500000000002 30.2375 25.45 18.475 18-19 25.15 25.85 26.075 22.925 20-21 25.6125 27.962500000000002 25.525 20.9 22-23 27.1625 23.7875 26.8 22.25 24-25 27.700000000000003 23.1375 26.887499999999996 22.275 26-27 25.0125 26.400000000000002 29.312500000000004 19.275000000000002 28-29 25.825 27.400000000000002 26.2625 20.5125 30-31 28.15 26.2875 24.4125 21.15 32-33 25.2 27.025 26.674999999999997 21.099999999999998 34-35 26.7125 24.575 27.1125 21.6 36-37 25.6125 23.4125 28.262500000000003 22.7125 38-39 25.1 24.637500000000003 31.137500000000003 19.125 40-41 26.0125 25.75 26.687499999999996 21.55 42-43 25.637500000000003 27.325 26.275 20.7625 44-45 24.8 24.6625 27.55 22.9875 46-47 24.975 22.7375 27.1125 25.174999999999997 48-49 25.5 24.0 29.599999999999998 20.9 50-51 25.974999999999998 25.3 28.487499999999997 20.2375 52-53 25.1 27.3625 26.0625 21.475 54-55 24.325 27.55 27.0875 21.0375 56-57 25.887500000000003 24.8625 27.5125 21.7375 58-59 24.525 23.175 28.799999999999997 23.5 60-61 25.424999999999997 23.0125 29.275000000000002 22.287499999999998 62-63 23.0375 27.0625 30.7875 19.112499999999997 64-65 25.0 25.112499999999997 28.225 21.6625 66-67 26.8125 26.4625 27.0875 19.6375 68-69 24.25 26.9125 27.212500000000002 21.625 70-71 27.160185069401027 22.933600100037513 27.285231961985744 22.620982868575716 72-73 26.529588766298893 22.617853560682047 27.657973921765294 23.19458375125376 74-75 24.343345481965564 26.504964182480833 28.113610657282894 21.038079678270705 76-77 23.464249748237663 25.85599194360524 29.607250755287005 21.07250755287009 78-79 24.041855774079675 24.117498739283914 30.395864851235505 21.44478063540091 80-81 24.21105781368341 26.63468821004797 29.954556930068165 19.199697046200452 82-83 24.687144482366325 24.383769434963977 29.288332701301982 21.640753381367716 84-85 24.857396374698947 23.71656737229053 30.269996197236658 21.15604005577386 86-87 23.63083164300203 25.747971602434077 31.50354969574036 19.11764705882353 88-89 23.364604462474645 28.182048681541584 29.107505070993916 19.345841784989858 90-91 27.281947261663287 24.543610547667345 28.16937119675456 20.00507099391481 92-93 23.047667342799187 27.903144016227184 28.93002028397566 20.11916835699797 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 6.5 18 7.0 19 1.5 20 1.5 21 2.0 22 2.0 23 2.5 24 5.5 25 5.5 26 6.5 27 8.5 28 13.5 29 20.0 30 23.0 31 27.0 32 35.5 33 46.0 34 56.5 35 65.5 36 81.5 37 106.5 38 130.5 39 145.0 40 147.0 41 168.5 42 192.0 43 194.5 44 192.5 45 188.0 46 216.0 47 205.0 48 182.5 49 198.5 50 183.0 51 158.5 52 154.0 53 177.0 54 153.0 55 101.0 56 77.5 57 66.0 58 69.0 59 66.0 60 59.0 61 54.0 62 48.5 63 45.5 64 45.0 65 39.0 66 31.0 67 24.0 68 19.5 69 14.0 70 8.5 71 10.0 72 8.0 73 6.0 74 3.0 75 0.0 76 1.0 77 1.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.2 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.06333924499619964 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 3.0 71 5.0 72 8.0 73 5.0 74 1.0 75 4.0 76 4.0 77 3.0 78 2.0 79 4.0 80 0.0 81 3.0 82 5.0 83 3.0 84 6.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3944.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.27499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 92.86373423561982 75.47500000000001 2 4.398646570286066 7.1499999999999995 3 1.1996308828052906 2.9250000000000003 4 0.3383574284835435 1.0999999999999999 5 0.3383574284835435 1.375 6 0.12303906490310675 0.6 7 0.06151953245155337 0.35000000000000003 8 0.09227929867733005 0.6 9 0.06151953245155337 0.44999999999999996 >10 0.492156259612427 8.025 >50 0.030759766225776686 1.95 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 78 1.95 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 49 1.225 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 41 1.0250000000000001 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 29 0.7250000000000001 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 26 0.65 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 22 0.5499999999999999 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 22 0.5499999999999999 No Hit GGAGTCCCATATATATATGTATAAGATGCCAGCCCGGTTTCGTCAACCAT 17 0.42500000000000004 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 17 0.42500000000000004 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 14 0.35000000000000003 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 14 0.35000000000000003 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 13 0.325 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 13 0.325 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 12 0.3 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 11 0.27499999999999997 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 11 0.27499999999999997 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 10 0.25 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 9 0.22499999999999998 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 9 0.22499999999999998 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 8 0.2 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 8 0.2 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 8 0.2 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 7 0.17500000000000002 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 7 0.17500000000000002 No Hit GGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTT 6 0.15 No Hit GGATATCGTGTGTGTACATTTGAATGTACCGACATGGGCTCGAGGAGCAT 6 0.15 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 6 0.15 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 6 0.15 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 5 0.125 No Hit GGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTAGATGCTGCCA 5 0.125 No Hit GAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGG 5 0.125 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 5 0.125 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 5 0.125 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 5 0.125 No Hit GAAAAAGGATCGGTCGATCATCGGAGAAGAACACTTCCTCCGTGCATATG 5 0.125 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 5 0.125 No Hit GGAGTAAACTAGCATAAATAATTTGTTTGATGCCAGTCATCAAGGTTGAG 5 0.125 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 5 0.125 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0125 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGAAG 20 0.0027298967 65.25 1 >>END_MODULE Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180295 READS because READLEN < 1 Read 180295 spots for ERR6133427.sra Written 180295 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra Rejected 180277 READS because READLEN < 1 Read 180277 spots for ERR6133427.sra Written 180277 spots for ERR6133427.sra SRR ids: ['ERR6133427.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_m8u3cj7z ERR6133427.sra spots: 3605558 blocks: [[1, 180277], [180278, 360554], [360555, 540831], [540832, 721108], [721109, 901385], [901386, 1081662], [1081663, 1261939], [1261940, 1442216], [1442217, 1622493], [1622494, 1802770], [1802771, 1983047], [1983048, 2163324], [2163325, 2343601], [2343602, 2523878], [2523879, 2704155], [2704156, 2884432], [2884433, 3064709], [3064710, 3244986], [3244987, 3425263], [3425264, 3605558]] ERR6133427 file size 798759 ERR6133427 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133427 ERR6133427_1.fastq Input file: ERR6133427_1.fastq trimmed: ERR6133427-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 05:07:39 2024 >> started Sat Dec 7 05:07:41 2024 >> done (2.025s) 3605558 reads processed; of these: 2027 ( 0.06%) short reads filtered out after trimming by size control 37 ( 0.00%) empty reads filtered out after trimming by size control 3603494 (99.94%) reads available; of these: 50721 ( 1.41%) trimmed reads available after processing 3552773 (98.59%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 46 0.00% 19 40 0.00% 20 34 0.00% 21 23 0.00% 22 21 0.00% 23 21 0.00% 24 14 0.00% 25 11 0.00% 26 9 0.00% 27 6 0.00% 28 12 0.00% 29 49 0.00% 30 11 0.00% 31 8 0.00% 32 9 0.00% 33 19 0.00% 34 2 0.00% 35 8 0.00% 36 6 0.00% 37 20 0.00% 38 15 0.00% 39 16 0.00% 40 32 0.00% 41 18 0.00% 42 20 0.00% 43 26 0.00% 44 24 0.00% 45 24 0.00% 46 22 0.00% 47 30 0.00% 48 22 0.00% 49 32 0.00% 50 26 0.00% 51 35 0.00% 52 26 0.00% 53 37 0.00% 54 25 0.00% 55 24 0.00% 56 17 0.00% 57 18 0.00% 58 22 0.00% 59 25 0.00% 60 16 0.00% 61 18 0.00% 62 0 0.00% 63 2 0.00% 64 3 0.00% 65 1 0.00% 66 5 0.00% 67 11 0.00% 68 21 0.00% 69 41 0.00% 70 4288 0.12% 71 3476 0.10% 72 3619 0.10% 73 3414 0.09% 74 3856 0.11% 75 3596 0.10% 76 3203 0.09% 77 3341 0.09% 78 3995 0.11% 79 4826 0.13% 80 3998 0.11% 81 4139 0.11% 82 4793 0.13% 83 5438 0.15% 84 4060 0.11% 85 89 0.00% 86 191 0.01% 87 346 0.01% 88 629 0.02% 89 1278 0.04% 90 2838 0.08% 91 8656 0.24% 92 34758 0.96% 93 3493644 96.95% 3603494 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.47 fanout-score-rank=32 prefix-density=0.59 prefix-fanout=2.2 sequence=TGTACATTTGAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=111.56 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=7.2 sequence=AGGAAGAGGAGGATGCAGTCAGGGCTCACAAACAACCTGCCACTGCCGCCATTGGCCTTCTAAAACAGGAGAGGAGGGGTTAGATAGTTTCGATCTGCAAGGGGGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGCAGAAGCTGTATGCTTATGAGCAGCTATGGTACTTATCGAGGACAGTAGCGTACTTGAGGTGTTGTATTTTTATTTATTT Started job on | Dec 07 05:07:58 Started mapping on | Dec 07 05:07:59 Finished on | Dec 07 05:08:04 Mapping speed, Million of reads per hour | 2594.52 Number of input reads | 3603494 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 2662793 Uniquely mapped reads % | 73.89% Average mapped length | 92.40 Number of splices: Total | 162790 Number of splices: Annotated (sjdb) | 137549 Number of splices: GT/AG | 157711 Number of splices: GC/AG | 2975 Number of splices: AT/AC | 64 Number of splices: Non-canonical | 2040 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 1.77 Insertion rate per base | 0.01% Insertion average length | 1.95 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 884374 % of reads mapped to multiple loci | 24.54% Number of reads mapped to too many loci | 10147 % of reads mapped to too many loci | 0.28% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.26% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 56327 56327 56327 N_multimapping 884374 884374 884374 N_noFeature 128679 154732 2548764 N_ambiguous 97667 9675 276 UnstrandedReadsAssigned:2436447 PositiveStrandReadsAssigned:2498386 NegativeStrandReadsAssigned:113753 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133427 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133427-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,603,494 reads, 3,187,823 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,054 rounds 52973 ERR6133427.ke.tsv 35125 ERR6133427.se.tsv 88098 total ==> ERR6133427.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 62 18.6075 PNS24243 293 194 0 0 KQK14069 1603 1504 14 3.83294 KQK14071 474 375 0 0 ==> ERR6133427.se.tsv <== BRADI_1g14170v3 14 BRADI_1g53295v3 59 BRADI_1g59795v3 12 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 54 BRADI_1g74790v3 38 BRADI_1g09890v3 0 BRADI_1g77505v3 74 BRADI_1g48960v3 0 ERR6133427 completed mapping pipeline successfully