Starting /dee2/code/volunteer_pipeline.sh ERR6133434 current disk space = 1545769570304 free memory = 1447023912 ERR6133434 SRAfilesize afec549b0347031653d8f11e97b813ee ERR6133434.sra ERR6133434.sra file validated ERR6133434 is single end ERR6133434 is conventional basespace ERR6133434 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133434_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.1525 37.0 33.0 37.0 33.0 37.0 2 36.3195 37.0 37.0 37.0 33.0 37.0 3 35.6545 37.0 37.0 37.0 33.0 37.0 4 35.40925 37.0 37.0 37.0 33.0 37.0 5 35.23175 37.0 37.0 37.0 33.0 37.0 6 35.48825 37.0 37.0 37.0 33.0 37.0 7 37.227 37.0 37.0 40.0 33.0 40.0 8 37.24325 37.0 37.0 40.0 33.0 40.0 9 37.188 37.0 37.0 40.0 33.0 40.0 10-11 37.184125 37.0 37.0 40.0 33.0 40.0 12-13 37.064 37.0 37.0 40.0 33.0 40.0 14-15 37.078375 37.0 37.0 40.0 33.0 40.0 16-17 37.050375 37.0 37.0 40.0 33.0 40.0 18-19 37.27875 37.0 37.0 40.0 33.0 40.0 20-21 37.377750000000006 37.0 37.0 40.0 33.0 40.0 22-23 37.294375 37.0 37.0 40.0 33.0 40.0 24-25 37.223124999999996 37.0 37.0 40.0 33.0 40.0 26-27 36.982124999999996 37.0 37.0 40.0 33.0 40.0 28-29 37.09075 37.0 37.0 40.0 33.0 40.0 30-31 37.022375 37.0 37.0 40.0 33.0 40.0 32-33 36.9565 37.0 37.0 40.0 33.0 40.0 34-35 36.692499999999995 37.0 37.0 40.0 33.0 40.0 36-37 36.643249999999995 37.0 37.0 40.0 33.0 40.0 38-39 36.553875 37.0 37.0 40.0 33.0 40.0 40-41 36.372375 37.0 37.0 40.0 33.0 40.0 42-43 36.30475 37.0 37.0 40.0 33.0 40.0 44-45 36.025625000000005 37.0 35.0 40.0 33.0 40.0 46-47 35.958 37.0 35.0 37.0 33.0 40.0 48-49 35.8395 37.0 33.0 37.0 33.0 40.0 50-51 35.641625 37.0 33.0 37.0 33.0 40.0 52-53 35.476 37.0 33.0 37.0 33.0 40.0 54-55 35.259 37.0 33.0 37.0 33.0 38.5 56-57 35.170500000000004 37.0 33.0 37.0 33.0 37.0 58-59 34.7695 37.0 33.0 37.0 30.0 37.0 60-61 34.670500000000004 37.0 33.0 37.0 33.0 37.0 62-63 34.686499999999995 37.0 33.0 37.0 33.0 37.0 64-65 34.150125 37.0 33.0 37.0 30.0 37.0 66-67 34.004875 37.0 33.0 37.0 27.0 37.0 68-69 32.829375 35.0 33.0 35.0 27.0 37.0 70-71 33.186435890222555 33.0 33.0 37.0 27.0 37.0 72-73 33.99250076174712 37.0 33.0 37.0 27.0 37.0 74-75 34.22339662172738 37.0 33.0 37.0 27.0 37.0 76-77 34.06375622547385 37.0 33.0 37.0 27.0 37.0 78-79 34.161836591792444 37.0 33.0 37.0 30.0 37.0 80-81 33.9452092438323 37.0 33.0 37.0 27.0 37.0 82-83 33.63829555768338 37.0 33.0 37.0 27.0 37.0 84-85 33.601586246176296 35.0 33.0 37.0 27.0 37.0 86-87 33.7373417721519 35.0 33.0 37.0 27.0 37.0 88-89 33.61151898734177 33.0 33.0 37.0 27.0 37.0 90-91 33.4426582278481 33.0 33.0 37.0 27.0 37.0 92-93 33.42620253164557 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 6.0 21 9.0 22 5.0 23 15.0 24 24.0 25 17.0 26 39.0 27 43.0 28 44.0 29 56.0 30 96.0 31 112.0 32 154.0 33 192.0 34 273.0 35 555.0 36 889.0 37 1063.0 38 402.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 89.225 3.025 1.95 5.800000000000001 2 74.04904904904906 16.59159159159159 5.63063063063063 3.7287287287287287 3 37.724999999999994 39.324999999999996 12.950000000000001 10.0 4 35.275 27.650000000000002 17.224999999999998 19.85 5 24.099999999999998 33.300000000000004 23.7 18.9 6 21.099999999999998 37.475 24.375 17.05 7 38.725 28.749999999999996 18.2 14.325 8 33.074999999999996 29.475 21.325 16.125 9 27.425 27.975 25.825 18.775 10-11 27.224999999999998 27.5125 26.3125 18.95 12-13 30.337500000000002 25.362499999999997 26.5 17.8 14-15 22.6875 28.249999999999996 28.625 20.4375 16-17 25.525 30.15 25.75 18.575 18-19 23.0 25.9875 26.9625 24.05 20-21 24.2 26.625 26.687499999999996 22.4875 22-23 27.675 23.3625 27.0875 21.875 24-25 27.237499999999997 24.1875 27.1625 21.4125 26-27 25.662499999999998 25.662499999999998 28.287499999999998 20.3875 28-29 27.800000000000004 25.424999999999997 26.200000000000003 20.575 30-31 27.487499999999997 25.0125 26.087500000000002 21.4125 32-33 25.2875 28.025 26.825 19.8625 34-35 26.1 24.5 27.9125 21.4875 36-37 25.2375 24.4875 28.6625 21.6125 38-39 26.8 25.3 28.525 19.375 40-41 27.200000000000003 25.1875 25.074999999999996 22.537499999999998 42-43 25.474999999999998 26.275 26.575 21.675 44-45 25.025 23.5375 28.275 23.1625 46-47 25.7375 23.2125 28.000000000000004 23.05 48-49 25.887500000000003 23.95 29.225 20.9375 50-51 26.087500000000002 25.5625 28.199999999999996 20.150000000000002 52-53 26.5125 26.0 26.424999999999997 21.0625 54-55 24.3875 26.825 28.037499999999998 20.75 56-57 26.150000000000002 25.45 27.400000000000002 21.0 58-59 25.5125 24.4125 28.025 22.05 60-61 24.5125 25.2125 28.275 22.0 62-63 24.325 26.5625 29.362500000000004 19.75 64-65 24.975 24.637500000000003 29.9 20.4875 66-67 24.7 25.674999999999997 28.487499999999997 21.1375 68-69 24.3125 25.95 28.1875 21.55 70-71 25.54069258657332 24.79059882485311 28.56607075884486 21.102637829728714 72-73 25.69461827284105 24.63078848560701 27.934918648310386 21.73967459324155 74-75 23.17914002757929 26.99009652751661 29.53491287451423 20.29585057038987 76-77 24.475963348813856 23.973892305761265 29.597087987950292 21.953056357474583 78-79 23.874779984913253 24.339954739753583 29.7208951470958 22.064370128237364 80-81 24.58747953142713 25.960448419196375 29.386572616198514 20.065499433177983 82-83 24.61208527816324 24.37239813296329 29.733821117699005 21.281695471174466 84-85 24.731182795698924 23.52941176470588 29.968374446552815 21.771030993042377 86-87 23.949367088607595 25.417721518987342 31.075949367088608 19.556962025316455 88-89 23.87341772151899 26.68354430379747 30.063291139240505 19.379746835443036 90-91 25.68354430379747 25.0126582278481 29.860759493670884 19.443037974683545 92-93 23.455696202531644 26.772151898734176 29.417721518987346 20.354430379746834 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 5.0 18 6.0 19 1.0 20 0.0 21 1.0 22 2.5 23 4.5 24 3.5 25 2.5 26 4.5 27 5.0 28 11.0 29 16.5 30 20.0 31 22.5 32 35.0 33 48.0 34 56.0 35 69.0 36 86.0 37 117.5 38 140.0 39 145.5 40 163.0 41 191.5 42 207.0 43 209.5 44 199.0 45 187.0 46 219.0 47 217.0 48 164.5 49 165.5 50 174.5 51 170.0 52 153.5 53 136.5 54 121.0 55 97.0 56 86.0 57 80.0 58 78.0 59 66.5 60 61.0 61 59.5 62 46.5 63 48.0 64 51.5 65 38.5 66 30.5 67 27.0 68 19.0 69 17.0 70 14.5 71 9.0 72 7.0 73 4.5 74 2.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.012648621300278268 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 1.0 71 2.0 72 4.0 73 3.0 74 3.0 75 2.0 76 3.0 77 3.0 78 4.0 79 4.0 80 3.0 81 2.0 82 5.0 83 5.0 84 6.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3950.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.9 #Duplication Level Percentage of deduplicated Percentage of total 1 94.53924914675767 83.1 2 3.668941979522184 6.45 3 0.7679180887372014 2.025 4 0.25597269624573377 0.8999999999999999 5 0.08532423208191127 0.375 6 0.08532423208191127 0.44999999999999996 7 0.08532423208191127 0.525 8 0.11376564277588168 0.8 9 0.11376564277588168 0.8999999999999999 >10 0.2844141069397042 4.475 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 45 1.125 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 20 0.5 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 20 0.5 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 17 0.42500000000000004 No Hit GGGTGAGCATATATATTTATACGACGAATAAAAGGCTGCCACGTGGCGGG 15 0.375 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 14 0.35000000000000003 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 13 0.325 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 12 0.3 No Hit GGAGAGGGTGAGCATATATATTTATACGACGAATAAAAGGCTGCCACGTG 12 0.3 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 11 0.27499999999999997 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 9 0.22499999999999998 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 9 0.22499999999999998 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 9 0.22499999999999998 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 9 0.22499999999999998 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 8 0.2 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 8 0.2 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 8 0.2 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 8 0.2 No Hit GGATCGGTTGAAGCGTGCGGCGTCTCTGTCTATGTATTACTGTTTTATGC 7 0.17500000000000002 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 7 0.17500000000000002 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 7 0.17500000000000002 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 5 0.125 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACTAG 15 9.0286706E-4 86.225006 7 CAGAACT 15 9.0286706E-4 86.225006 5 >>END_MODULE Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286583 READS because READLEN < 1 Read 286583 spots for ERR6133434.sra Written 286583 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra Rejected 286580 READS because READLEN < 1 Read 286580 spots for ERR6133434.sra Written 286580 spots for ERR6133434.sra SRR ids: ['ERR6133434.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wf9lezd8 ERR6133434.sra spots: 5731603 blocks: [[1, 286580], [286581, 573160], [573161, 859740], [859741, 1146320], [1146321, 1432900], [1432901, 1719480], [1719481, 2006060], [2006061, 2292640], [2292641, 2579220], [2579221, 2865800], [2865801, 3152380], [3152381, 3438960], [3438961, 3725540], [3725541, 4012120], [4012121, 4298700], [4298701, 4585280], [4585281, 4871860], [4871861, 5158440], [5158441, 5445020], [5445021, 5731603]] ERR6133434 file size 1271455 ERR6133434 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133434 ERR6133434_1.fastq Input file: ERR6133434_1.fastq trimmed: ERR6133434-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 05:28:13 2024 >> started Sat Dec 7 05:28:29 2024 >> done (15.996s) 5731603 reads processed; of these: 1685 ( 0.03%) short reads filtered out after trimming by size control 22 ( 0.00%) empty reads filtered out after trimming by size control 5729896 (99.97%) reads available; of these: 85762 ( 1.50%) trimmed reads available after processing 5644134 (98.50%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 32 0.00% 19 22 0.00% 20 24 0.00% 21 14 0.00% 22 14 0.00% 23 8 0.00% 24 14 0.00% 25 11 0.00% 26 1 0.00% 27 7 0.00% 28 4 0.00% 29 44 0.00% 30 9 0.00% 31 11 0.00% 32 12 0.00% 33 3 0.00% 34 4 0.00% 35 5 0.00% 36 5 0.00% 37 9 0.00% 38 7 0.00% 39 27 0.00% 40 31 0.00% 41 14 0.00% 42 14 0.00% 43 37 0.00% 44 15 0.00% 45 12 0.00% 46 22 0.00% 47 19 0.00% 48 20 0.00% 49 19 0.00% 50 17 0.00% 51 31 0.00% 52 20 0.00% 53 20 0.00% 54 25 0.00% 55 27 0.00% 56 30 0.00% 57 14 0.00% 58 25 0.00% 59 23 0.00% 60 17 0.00% 61 18 0.00% 62 2 0.00% 63 5 0.00% 64 2 0.00% 65 5 0.00% 66 8 0.00% 67 11 0.00% 68 29 0.00% 69 50 0.00% 70 4923 0.09% 71 5218 0.09% 72 5397 0.09% 73 5052 0.09% 74 5218 0.09% 75 5217 0.09% 76 4945 0.09% 77 5278 0.09% 78 5365 0.09% 79 5986 0.10% 80 5484 0.10% 81 5586 0.10% 82 6216 0.11% 83 6647 0.12% 84 5794 0.10% 85 200 0.00% 86 353 0.01% 87 565 0.01% 88 1077 0.02% 89 2049 0.04% 90 4761 0.08% 91 14230 0.25% 92 60294 1.05% 93 5563172 97.09% 5729896 reads passed initial QC criterion=sequence-density sequence-density=1.42 sequence-density-rank=1 fanout-score=3.23 fanout-score-rank=37 prefix-density=1.67 prefix-fanout=2.7 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=74.28 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=7.2 sequence=AGGAAGAGGAGGATGCAGTCAGGGCTCACAAACAACCTGCCACTGCCGCCATTGGCCTTCTAAAACAGGAGAGGAGGGGTTAGATAGTTTCGATCTGCAAGGGGGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGCAGAAGCTGTATGCTTATGAGCAGCTATGGTACTTATCGAGGACAGTAGCGTACTTGAGGTGTTGTATTTTTATTTATTT Started job on | Dec 07 05:30:02 Started mapping on | Dec 07 05:30:03 Finished on | Dec 07 05:30:52 Mapping speed, Million of reads per hour | 420.97 Number of input reads | 5729896 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 4751884 Uniquely mapped reads % | 82.93% Average mapped length | 92.47 Number of splices: Total | 389434 Number of splices: Annotated (sjdb) | 338317 Number of splices: GT/AG | 381372 Number of splices: GC/AG | 6192 Number of splices: AT/AC | 94 Number of splices: Non-canonical | 1776 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 1.74 Insertion rate per base | 0.01% Insertion average length | 2.00 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 890824 % of reads mapped to multiple loci | 15.55% Number of reads mapped to too many loci | 14843 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.24% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 87188 87188 87188 N_multimapping 890824 890824 890824 N_noFeature 216738 256872 4558373 N_ambiguous 170303 17171 404 UnstrandedReadsAssigned:4364843 PositiveStrandReadsAssigned:4477841 NegativeStrandReadsAssigned:193107 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133434 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133434-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 5,729,896 reads, 5,151,816 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,102 rounds 52973 ERR6133434.ke.tsv 35125 ERR6133434.se.tsv 88098 total ==> ERR6133434.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 153 28.5139 PNS24243 293 194 0 0 KQK14069 1603 1504 98 16.6608 KQK14071 474 375 0 0 ==> ERR6133434.se.tsv <== BRADI_1g14170v3 99 BRADI_1g53295v3 78 BRADI_1g59795v3 46 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 83 BRADI_1g74790v3 41 BRADI_1g09890v3 0 BRADI_1g77505v3 136 BRADI_1g48960v3 0 ERR6133434 completed mapping pipeline successfully